IL6 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	IL6
Entrez Gene ID	403985
Full Name	interleukin 6
Synonyms	IL-6
General protein information	Preferred Names interleukin 6 Names interleukin-6 interleukin 6 (interferon, beta 2)
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001003301.1	NP_001003301.1	interleukin-6 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	IL6	NP_000591.1
Mus musculus (house mouse)	Il6	NP_112445.1
Gallus gallus (chicken)	IL6	NP_989959.1
Pan troglodytes (chimpanzee)	IL6	XP_518992.2
Canis lupus familiaris (dog)	IL6	NP_001003301.1
	IL6	NP_001036198.1
Rattus norvegicus (Norway rat)	Il6	NP_036721.1

Gene Ontology
Bibliography

Related articles in PubMed

Increased CSF aquaporin-4, and interleukin-6 levels in dogs with idiopathic communicating internal hydrocephalus and a decrease after ventriculo-peritoneal shunting.
Schmidt MJ, Rummel C, Hauer J, Kolecka M, Ondreka N, McClure V, Roth J
Fluids and barriers of the CNS13(1)12(2016 Jun)

Interleukin-6 as a prognostic marker in dogs in an intensive care unit.
Sch?ttler J, Neumann S
Veterinary clinical pathology44(2)223-8(2015 Jun)

Propofol attenuates LPS-induced tumor necrosis factor-?, interleukin-6 and nitric oxide expression in canine peripheral blood mononuclear cells possibly through down-regulation of nuclear factor (NF)-?B activation.
Pei Z, Wang J
The Journal of veterinary medical science77(2)139-45(2015 Feb)

Synoviocyte neotissues towards in vitro meniscal tissue engineering.
Warnock JJ, Duesterdieck-Zellmer KF, Bobe G, Baltzer WI, Ott J
Research in veterinary science95(3)1201-9(2013 Dec)

A potential role for substance P and interleukin-6 in the cerebrospinal fluid of Cavalier King Charles Spaniels with neuropathic pain.
Schmidt MJ, Roth J, Ondreka N, Kramer M, Rummel C
Journal of veterinary internal medicine27(3)530-5(2013 May-Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

An increase in ventricular dimension is accompanied by increases in AQP4 and IL-6 concentrations in the CSF of dogs with idiopathic communicating hydrocephalus. All were greatly reduced or normalised after shunt treatment. Therefore AQP4 and IL-6 in CSF can be indicators of ventriculomegaly, of cellular damage, and of improvement after the treatment of hydrocephalus.
Title: Increased CSF aquaporin-4, and interleukin-6 levels in dogs with idiopathic communicating internal hydrocephalus and a decrease after ventriculo-peritoneal shunting.

The results of this study suggest that IL-6 is a useful prognostic marker for canine critical care patients with acute internal disease.
Title: Interleukin-6 as a prognostic marker in dogs in an intensive care unit.

This diminished TNF-alpha, IL-6 and iNOS expression, and NO production was in parallel to the respective decreased NF-kappaB p65 protein nuclear translocation in the LPS-activated canine PBMCs pretreated with 25 microM and 50 microM propofol.
Title: Propofol attenuates LPS-induced tumor necrosis factor-?, interleukin-6 and nitric oxide expression in canine peripheral blood mononuclear cells possibly through down-regulation of nuclear factor (NF)-?B activation.

Release of interleukin-6 and substance P may initiate proinflammatory effects leading to development of persistent pain in dogs with syringomyelia.
Title: A potential role for substance P and interleukin-6 in the cerebrospinal fluid of Cavalier King Charles Spaniels with neuropathic pain.

The etiology of blindness in dogs with pituitary dependent hypercortisolism, including the role of adiponectin, interleukin 6, insulin, and nitric oxide, is reported.
Title: Interleukin-6 and insulin incrase and nitric oxide and adiponectin decrease in blind dogs with pituitary-dependent hyperadrenocorticism.

The following IL6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IL6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe00344	NM_001003301.1 Latest version!	Canis lupus familiaris interleukin 6 (IL6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IL6 ( NM_001003301.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001003301.1 Canis lupus familiaris interleukin 6 (IL6), mRNA.

Price & Availability↑

CloneID	OCe00344
Clone ID Related Accession (Same CDS sequence)	NM_001003301.1
Accession Version	NM_001003301.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Canis lupus familiaris(dog)
Product	interleukin-6 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U12234.1. On Jan 12, 2005 this sequence version replaced XM_532488.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U12234.1, AF275796.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END##

ATGAACTCCC TCTCCACAAG CGCCTTCTCC CTGGGGCTGC TCCTGGTGAT GGCTACTGCT 
TTCCCTACCC CGGGACCCCT GGCAGGAGAT TCCAAGGATG ATGCCACTTC AAATAGTCTA 
CCACTCACCT CTGCAAACAA AGTGGAAGAA CTGATTAAGT ACATCCTCGG CAAAATCTCT 
GCACTGAGAA AGGAGATGTG TGACAAGTTT AACAAGTGTG AAGACAGCAA AGAGGCACTG 
GCAGAAAATA ACCTACATCT TCCCAAACTG GAGGGAAAAG ATGGATGCTT CCAATCTGGG 
TTCAATCAGG AGACCTGCTT GACAAGAATC ACTACCGGTC TTGTGGAGTT TCAGCTACAC 
CTGAATATCC TCCAGAACAA CTATGAGGGT GATAAGGAAA ATGTCAAGTC TGTGCACATG 
AGTACCAAGA TCCTGGTCCA GATGCTAAAG AGCAAGGTAA AGAATCAGGA TGAAGTGACC 
ACTCCTGACC CAACCACAGA CGCCAGCCTG CAGGCTATCT TGCAGTCGCA GGATGAGTGC 
GTGAAGCACA CAACAATTCA CCTCATCCTG CGGAGTCTGG AGGATTTCCT GCAGTTCAGT 
CTGAGGGCTG TTCGGATAAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003301.1
CDS	58..681
Translation	MNSLSTSAFSLGLLLVMATAFPTPGPLAGDSKDDATSNSLPLTSANKVEELIKYILGKISALRKEMCDKFNKCEDSKEALAENNLHLPKLEGKDGCFQSGFNQETCLTRITTGLVEFQLHLNILQNNYEGDKENVKSVHMSTKILVQMLKSKVKNQDEVTTPDPTTDASLQAILQSQDECVKHTTIHLILRSLEDFLQFSLRAVRIM

Target ORF information:

RefSeq Version	NM_001003301.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris interleukin 6 (IL6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003301.1

ORF Insert Sequence:

ATGAACTCCC TCTCCACAAG CGCCTTCTCC CTGGGGCTGC TCCTGGTGAT GGCTACTGCT 
TTCCCTACCC CGGGACCCCT GGCAGGAGAT TCCAAGGATG ATGCCACTTC AAATAGTCTA 
CCACTCACCT CTGCAAACAA AGTGGAAGAA CTGATTAAGT ACATCCTCGG CAAAATCTCT 
GCACTGAGAA AGGAGATGTG TGACAAGTTT AACAAGTGTG AAGACAGCAA AGAGGCACTG 
GCAGAAAATA ACCTACATCT TCCCAAACTG GAGGGAAAAG ATGGATGCTT CCAATCTGGG 
TTCAATCAGG AGACCTGCTT GACAAGAATC ACTACCGGTC TTGTGGAGTT TCAGCTACAC 
CTGAATATCC TCCAGAACAA CTATGAGGGT GATAAGGAAA ATGTCAAGTC TGTGCACATG 
AGTACCAAGA TCCTGGTCCA GATGCTAAAG AGCAAGGTAA AGAATCAGGA TGAAGTGACC 
ACTCCTGACC CAACCACAGA CGCCAGCCTG CAGGCTATCT TGCAGTCGCA GGATGAGTGC 
GTGAAGCACA CAACAATTCA CCTCATCCTG CGGAGTCTGG AGGATTTCCT GCAGTTCAGT 
CTGAGGGCTG TTCGGATAAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Increased CSF aquaporin-4, and interleukin-6 levels in dogs with idiopathic communicating internal hydrocephalus and a decrease after ventriculo-peritoneal shunting.
Fluids and barriers of the CNS13(1)12(2016 Jun)
Schmidt MJ,Rummel C,Hauer J,Kolecka M,Ondreka N,McClure V,Roth J

Interleukin-6 as a prognostic marker in dogs in an intensive care unit.
Veterinary clinical pathology44(2)223-8(2015 Jun)
Sch?ttler J,Neumann S

Propofol attenuates LPS-induced tumor necrosis factor-?, interleukin-6 and nitric oxide expression in canine peripheral blood mononuclear cells possibly through down-regulation of nuclear factor (NF)-?B activation.
The Journal of veterinary medical science77(2)139-45(2015 Feb)
Pei Z,Wang J

Synoviocyte neotissues towards in vitro meniscal tissue engineering.
Research in veterinary science95(3)1201-9(2013 Dec)
Warnock JJ,Duesterdieck-Zellmer KF,Bobe G,Baltzer WI,Ott J

A potential role for substance P and interleukin-6 in the cerebrospinal fluid of Cavalier King Charles Spaniels with neuropathic pain.
Journal of veterinary internal medicine27(3)530-5(2013 May-Jun)
Schmidt MJ,Roth J,Ondreka N,Kramer M,Rummel C