YBX3 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	YBX3
Entrez Gene ID	403728
Full Name	Y-box binding protein 3
Synonyms	CSDA,ZONAB,dpbA
General protein information	Preferred Names Y-box binding protein 3 Names Y-box-binding protein 3 Y-box protein ZONAB-A cold shock domain protein A
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001003127.1	NP_001003127.1	Y-box-binding protein 3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CSDA	NP_001003127.1
Pan troglodytes (chimpanzee)	CSDA	XP_520744.3
Mus musculus (house mouse)	Ybx3	NP_620817.2
Gallus gallus (chicken)	CSDA	NP_990737.1
Homo sapiens (human)	YBX3	NP_003642.3
Rattus norvegicus (Norway rat)	Ybx3	NP_114185.3
Xenopus tropicalis (tropical clawed frog)	ybx3	NP_001015855.1
	LOC722347	XP_001118505.1
Bos taurus (cattle)	CSDA	NP_001091543.1

Gene Ontology
Bibliography

Related articles in PubMed

ZO proteins redundantly regulate the transcription factor DbpA/ZONAB.
Spadaro D, Tapia R, Jond L, Sudol M, Fanning AS, Citi S
The Journal of biological chemistry289(32)22500-11(2014 Aug)

The Y-box factor ZONAB/DbpA associates with GEF-H1/Lfc and mediates Rho-stimulated transcription.
Nie M, Aijaz S, Leefa Chong San IV, Balda MS, Matter K
EMBO reports10(10)1125-31(2009 Oct)

The heat-shock protein Apg-2 binds to the tight junction protein ZO-1 and regulates transcriptional activity of ZONAB.
Tsapara A, Matter K, Balda MS
Molecular biology of the cell17(3)1322-30(2006 Mar)

RalA interacts with ZONAB in a cell density-dependent manner and regulates its transcriptional activity.
Frankel P, Aronheim A, Kavanagh E, Balda MS, Matter K, Bunney TD, Marshall CJ
The EMBO journal24(1)54-62(2005 Jan)

A method for the large-scale cloning of nuclear proteins and nuclear targeting sequences on a functional basis.
Pichon B, Mercan D, Pouillon V, Christophe-Hobertus C, Christophe D
Analytical biochemistry284(2)231-9(2000 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

examined the localization and activity of DbpA and YAP in Madin-Darby canine kidney cells depleted either of ZO-1, or one of the related proteins ZO-2 and ZO-3 (ZO proteins), or all three together
Title: ZO proteins redundantly regulate the transcription factor DbpA/ZONAB.

data thus indicate that GEF-H1 and ZONAB form a signalling module that mediates Rho-regulated cyclin D1 promoter activation and expression.
Title: The Y-box factor ZONAB/DbpA associates with GEF-H1/Lfc and mediates Rho-stimulated transcription.

Apg-2 regulates ZONAB function by competing for binding to the SH3 domain of ZO-1 and suggest that Apg-2 functions as a regulator of ZO-1-ZONAB signaling in epithelial cells in response to cellular stress.
Title: The heat-shock protein Apg-2 binds to the tight junction protein ZO-1 and regulates transcriptional activity of ZONAB.

data presented here implicate the RalA/ZONAB interaction in the regulation of ZONAB function
Title: RalA interacts with ZONAB in a cell density-dependent manner and regulates its transcriptional activity.

The following YBX3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the YBX3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe33838	NM_001003127.1 Latest version!	Canis lupus familiaris Y-box binding protein 3 (YBX3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

YBX3 ( NM_001003127.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001003127.1 Canis lupus familiaris Y-box binding protein 3 (YBX3), mRNA.

Price & Availability↑

CloneID	OCe33838
Clone ID Related Accession (Same CDS sequence)	NM_001003127.1
Accession Version	NM_001003127.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-21
Organism	Canis lupus familiaris(dog)
Product	Y-box-binding protein 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF171061.1. ##Evidence-Data-START## Transcript exon combination :: AF171061.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END##

ATGAGCGAGG CGGGCGAGGC CACCGGCACC ACCAGCAGCA GCAGCAGCAG CCTCCCGCAG 
GCTCCGGCGG AGGCGGCCCC CGCGGCGCCC CAGGACCCCG CGCCCAAGAG CCCGGGGGGC 
AGCGGCGCGC CCCAGGCCGC GGCCCCGCCG CCCGCCGCGC TCGCCGCGGG AAACCCCGGT 
GGGGACGCGG CCCCCGCAGC CACGGGCACC GCGGCCCCCG CCTCTTCGGC CCCCGCCGGC 
GGTGAGGACG CGGAGAAGAA AGTTCTCGCC ACCAAAGTCC TTGGCACTGT CAAATGGTTC 
AACGTCAGAA ATGGATATGG ATTTATAAAT CGAAATGACA CCAAAGAAGA TGTATTTGTA 
CATCACACTG CCATCAAGAA GAATAACCCA CGGAAATACC TGCGCAGTGT AGGAGATGGA 
GAAACTGTAG AATTTGATGT GGTTGAAGGA GAGAAGGGTG CAGAAGCAGC CAATGTGACT 
GGACCAGACG GAGTTCCTGT AGAAGGGAGC CGTTACGCTG CTGATCGGCG TCGATACAGA 
CGTGGTTACT ATGGCAGACG CCGTGGACCT CCCCGTAATG CCGGTGAGAT TGGAGAGATG 
AAGGAGGGAG TCCCAGAGGG GACACAGCTG CAGGGACCGG TTCATCGAAA TCCAACTTAC 
CGCCCAAGGT ACCGGAGAGG CCCTCCTCGC CCCCGACCTG CCCCAGCTGT TGGTGAGGCT 
GAAGATAAAG AGAACCAACA AGCAGCCAAT GGTCCAAACC AGCCATCTGC TCGCCGTGGA 
TACCGGCGCC CATACAACTA TCGGCGTCGC CCCCGCCCCC CTAATGCTCC TTCACAAGAT 
GGCAAAGAGA CCAAGGCACC TGAAGTTCCA TCTGAGAACC CCGCTCCAGC CACCGAGCAG 
AGCAGTGCTG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003127.1
CDS	247..1161
Translation	MSEAGEATGTTSSSSSSLPQAPAEAAPAAPQDPAPKSPGGSGAPQAAAPPPAALAAGNPGGDAAPAATGTAAPASSAPAGGEDAEKKVLATKVLGTVKWFNVRNGYGFINRNDTKEDVFVHHTAIKKNNPRKYLRSVGDGETVEFDVVEGEKGAEAANVTGPDGVPVEGSRYAADRRRYRRGYYGRRRGPPRNAGEIGEMKEGVPEGTQLQGPVHRNPTYRPRYRRGPPRPRPAPAVGEAEDKENQQAANGPNQPSARRGYRRPYNYRRRPRPPNAPSQDGKETKAPEVPSENPAPATEQSSAE

Target ORF information:

RefSeq Version	NM_001003127.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris Y-box binding protein 3 (YBX3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003127.1

ORF Insert Sequence:

ATGAGCGAGG CGGGCGAGGC CACCGGCACC ACCAGCAGCA GCAGCAGCAG CCTCCCGCAG 
GCTCCGGCGG AGGCGGCCCC CGCGGCGCCC CAGGACCCCG CGCCCAAGAG CCCGGGGGGC 
AGCGGCGCGC CCCAGGCCGC GGCCCCGCCG CCCGCCGCGC TCGCCGCGGG AAACCCCGGT 
GGGGACGCGG CCCCCGCAGC CACGGGCACC GCGGCCCCCG CCTCTTCGGC CCCCGCCGGC 
GGTGAGGACG CGGAGAAGAA AGTTCTCGCC ACCAAAGTCC TTGGCACTGT CAAATGGTTC 
AACGTCAGAA ATGGATATGG ATTTATAAAT CGAAATGACA CCAAAGAAGA TGTATTTGTA 
CATCACACTG CCATCAAGAA GAATAACCCA CGGAAATACC TGCGCAGTGT AGGAGATGGA 
GAAACTGTAG AATTTGATGT GGTTGAAGGA GAGAAGGGTG CAGAAGCAGC CAATGTGACT 
GGACCAGACG GAGTTCCTGT AGAAGGGAGC CGTTACGCTG CTGATCGGCG TCGATACAGA 
CGTGGTTACT ATGGCAGACG CCGTGGACCT CCCCGTAATG CCGGTGAGAT TGGAGAGATG 
AAGGAGGGAG TCCCAGAGGG GACACAGCTG CAGGGACCGG TTCATCGAAA TCCAACTTAC 
CGCCCAAGGT ACCGGAGAGG CCCTCCTCGC CCCCGACCTG CCCCAGCTGT TGGTGAGGCT 
GAAGATAAAG AGAACCAACA AGCAGCCAAT GGTCCAAACC AGCCATCTGC TCGCCGTGGA 
TACCGGCGCC CATACAACTA TCGGCGTCGC CCCCGCCCCC CTAATGCTCC TTCACAAGAT 
GGCAAAGAGA CCAAGGCACC TGAAGTTCCA TCTGAGAACC CCGCTCCAGC CACCGAGCAG 
AGCAGTGCTG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ZO proteins redundantly regulate the transcription factor DbpA/ZONAB.
The Journal of biological chemistry289(32)22500-11(2014 Aug)
Spadaro D,Tapia R,Jond L,Sudol M,Fanning AS,Citi S

The Y-box factor ZONAB/DbpA associates with GEF-H1/Lfc and mediates Rho-stimulated transcription.
EMBO reports10(10)1125-31(2009 Oct)
Nie M,Aijaz S,Leefa Chong San IV,Balda MS,Matter K

The heat-shock protein Apg-2 binds to the tight junction protein ZO-1 and regulates transcriptional activity of ZONAB.
Molecular biology of the cell17(3)1322-30(2006 Mar)
Tsapara A,Matter K,Balda MS

RalA interacts with ZONAB in a cell density-dependent manner and regulates its transcriptional activity.
The EMBO journal24(1)54-62(2005 Jan)
Frankel P,Aronheim A,Kavanagh E,Balda MS,Matter K,Bunney TD,Marshall CJ

A method for the large-scale cloning of nuclear proteins and nuclear targeting sequences on a functional basis.
Analytical biochemistry284(2)231-9(2000 Sep)
Pichon B,Mercan D,Pouillon V,Christophe-Hobertus C,Christophe D