RHOB cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	RHOB
Entrez Gene ID	403670
Full Name	ras homolog family member B
Synonyms	RHO
General protein information	Preferred Names ras homolog family member B Names rho-related GTP-binding protein RhoB ras homolog gene family, member B ras-related rho protein
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001287052.1	NP_001273981.1	rho-related GTP-binding protein RhoB

Pathways from BioSystems

Homologies

	RHOB	XP_001096298.1
Gallus gallus (chicken)	RHOB	NP_990240.1
Pan troglodytes (chimpanzee)	RHOB	XP_003308954.1
Canis lupus familiaris (dog)	RHOB	XP_540101.3
Bos taurus (cattle)	RHOB	NP_001071390.1
Xenopus tropicalis (tropical clawed frog)	rhob	NP_001001214.1
Homo sapiens (human)	RHOB	NP_004031.1
Mus musculus (house mouse)	Rhob	NP_031509.1
Rattus norvegicus (Norway rat)	Rhob	NP_071987.1

Gene Ontology
Bibliography

Related articles in PubMed

RhoB-dependent modulation of postendocytic traffic in polarized Madin-Darby canine kidney cells.
Rondanino C, Rojas R, Ruiz WG, Wang E, Hughey RP, Dunn KW, Apodaca G
Traffic (Copenhagen, Denmark)8(7)932-49(2007 Jul)

A method for the large-scale cloning of nuclear proteins and nuclear targeting sequences on a functional basis.
Pichon B, Mercan D, Pouillon V, Christophe-Hobertus C, Christophe D
Analytical biochemistry284(2)231-9(2000 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RhoB is localized, in part, to early endosomes where it regulates receptor egress through the early endocytic system
Title: RhoB-dependent modulation of postendocytic traffic in polarized Madin-Darby canine kidney cells.

The following RHOB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RHOB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe00450	NM_001287052.1 Latest version!	Canis lupus familiaris ras homolog family member B (RHOB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RHOB ( NM_001287052.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001273981.1 Canis lupus familiaris ras homolog family member B (RHOB), mRNA.

Price & Availability↑

CloneID	OCe00450
Clone ID Related Accession (Same CDS sequence)	NM_001287052.1
Accession Version	NM_001287052.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Canis lupus familiaris(dog)
Product	rho-related GTP-binding protein RhoB
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAEX03010763.1. On Nov 23, 2013 this sequence version replaced XM_540101.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments and orthologous data.

ATGGCGGCCA TCCGCAAGAA GCTGGTGGTG GTGGGCGACG GCGCGTGCGG CAAGACGTGC 
CTGCTGATCG TGTTCAGTAA GGACGAGTTC CCCGAGGTGT ACGTGCCCAC CGTCTTCGAG 
AACTATGTGG CCGACATCGA GGTGGACGGC AAGCAGGTGG AGCTGGCGCT CTGGGACACG 
GCGGGCCAGG AGGACTACGA CCGCCTGCGG CCACTCTCCT ACCCGGACAC CGACGTGATC 
CTCATGTGCT TCTCCGTGGA CAGCCCCGAC TCGCTGGAGA ACATCCCCGA GAAGTGGGTG 
CCCGAGGTGA AGCACTTCTG CCCCAACGTG CCCATCATCC TGGTGGCCAA CAAGAAAGAC 
CTGCGCAGCG ACGAGCACGT CCGCACGGAG CTGGCGCGCA TGAAGCAGGA ACCGGTGCGC 
ACGGATGACG GCCGCGCCAT GGCCGTGCGC ATCCAAGCCT ACGAATACCT CGAGTGCTCG 
GCCAAGACCA AGGAGGGCGT GCGCGAGGTC TTCGAGACGG CCACGCGCGC CGCGCTGCAG 
AAGCGCTACG GCTCCCAGAA CGGCTGCATC AACTGCTGCA AGGTGCTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273981.1
CDS	400..990
Translation	MAAIRKKLVVVGDGACGKTCLLIVFSKDEFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSVDSPDSLENIPEKWVPEVKHFCPNVPIILVANKKDLRSDEHVRTELARMKQEPVRTDDGRAMAVRIQAYEYLECSAKTKEGVREVFETATRAALQKRYGSQNGCINCCKVL

Target ORF information:

RefSeq Version	NM_001287052.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ras homolog family member B (RHOB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287052.1

ORF Insert Sequence:

ATGGCGGCCA TCCGCAAGAA GCTGGTGGTG GTGGGCGACG GCGCGTGCGG CAAGACGTGC 
CTGCTGATCG TGTTCAGTAA GGACGAGTTC CCCGAGGTGT ACGTGCCCAC CGTCTTCGAG 
AACTATGTGG CCGACATCGA GGTGGACGGC AAGCAGGTGG AGCTGGCGCT CTGGGACACG 
GCGGGCCAGG AGGACTACGA CCGCCTGCGG CCACTCTCCT ACCCGGACAC CGACGTGATC 
CTCATGTGCT TCTCCGTGGA CAGCCCCGAC TCGCTGGAGA ACATCCCCGA GAAGTGGGTG 
CCCGAGGTGA AGCACTTCTG CCCCAACGTG CCCATCATCC TGGTGGCCAA CAAGAAAGAC 
CTGCGCAGCG ACGAGCACGT CCGCACGGAG CTGGCGCGCA TGAAGCAGGA ACCGGTGCGC 
ACGGATGACG GCCGCGCCAT GGCCGTGCGC ATCCAAGCCT ACGAATACCT CGAGTGCTCG 
GCCAAGACCA AGGAGGGCGT GCGCGAGGTC TTCGAGACGG CCACGCGCGC CGCGCTGCAG 
AAGCGCTACG GCTCCCAGAA CGGCTGCATC AACTGCTGCA AGGTGCTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RhoB-dependent modulation of postendocytic traffic in polarized Madin-Darby canine kidney cells.
Traffic (Copenhagen, Denmark)8(7)932-49(2007 Jul)
Rondanino C,Rojas R,Ruiz WG,Wang E,Hughey RP,Dunn KW,Apodaca G

A method for the large-scale cloning of nuclear proteins and nuclear targeting sequences on a functional basis.
Analytical biochemistry284(2)231-9(2000 Sep)
Pichon B,Mercan D,Pouillon V,Christophe-Hobertus C,Christophe D