RHOB cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	RHOB
Entrez Gene ID	395734
Full Name	ras homolog family member B
General protein information	Preferred Names ras homolog family member B Names rho-related GTP-binding protein RhoB GTP-binding protein ras homolog gene family, member B
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_204909.1	NP_990240.1	rho-related GTP-binding protein RhoB

Pathways from BioSystems

Homologies

	RHOB	XP_001096298.1
Gallus gallus (chicken)	RHOB	NP_990240.1
Pan troglodytes (chimpanzee)	RHOB	XP_003308954.1
Canis lupus familiaris (dog)	RHOB	XP_540101.3
Bos taurus (cattle)	RHOB	NP_001071390.1
Xenopus tropicalis (tropical clawed frog)	rhob	NP_001001214.1
Homo sapiens (human)	RHOB	NP_004031.1
Mus musculus (house mouse)	Rhob	NP_031509.1
Rattus norvegicus (Norway rat)	Rhob	NP_071987.1

Gene Ontology
Bibliography

Related articles in PubMed

Small GTP-binding protein RhoB is expressed in glial M?ller cells in the vertebrate retina.
Santos-Bredariol AS, Belmonte MA, Kihara AH, Santos MF, Hamassaki DE
The Journal of comparative neurology494(6)976-85(2006 Feb)

LSox5 regulates RhoB expression in the neural tube and promotes generation of the neural crest.
Perez-Alcala S, Nieto MA, Barbas JA
Development (Cambridge, England)131(18)4455-65(2004 Sep)

Relative expression of Slug, RhoB, and HNK-1 in the cranial neural crest of the early chicken embryo.
Del Barrio MG, Nieto MA
Developmental dynamics : an official publication of the American Association of Anatomists229(1)136-9(2004 Jan)

Differential expression of distinct members of Rho family GTP-binding proteins during neuronal development: identification of Rac1B, a new neural-specific member of the family.
Malosio ML, Gilardelli D, Paris S, Albertinazzi C, de Curtis I
The Journal of neuroscience : the official journal of the Society for Neuroscience17(17)6717-28(1997 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data suggest that, although RhoB is expressed by Muller cells in chick and mouse, its role in the maintenance of cellular morphology and regulation may be different.
Title: Small GTP-binding protein RhoB is expressed in glial M?ller cells in the vertebrate retina.

Misexpression of LSox5 in the cephalic neural tube activated RhoB expression throughout the dorsoventral axis
Title: LSox5 regulates RhoB expression in the neural tube and promotes generation of the neural crest.

expression in the cranial neural crest of the early embryo
Title: Relative expression of Slug, RhoB, and HNK-1 in the cranial neural crest of the early chicken embryo.

The following RHOB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RHOB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa01100	NM_204909.1 Latest version!	Gallus gallus ras homolog family member B (RHOB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RHOB ( NM_204909.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_990240.1 Gallus gallus ras homolog family member B (RHOB), mRNA.

Price & Availability↑

CloneID	OGa01100
Clone ID Related Accession (Same CDS sequence)	NM_204909.1
Accession Version	NM_204909.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-05-13
Organism	Gallus gallus(chicken)
Product	rho-related GTP-binding protein RhoB
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF098515.1. ##Evidence-Data-START## Transcript exon combination :: AF098515.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCCGCCA TCCGCAAGAA GCTGGTGGTG GTGGGAGACG GCGCCTGTGG CAAGACCTGC 
CTCCTCATCG TCTTCAGCAA GGACGAGTTC CCCGAGGTTT ACGTGCCCAC CGTCTTTGAG 
AACTACGTGG CCGACATCGA GGTGGACGGC AAGCAGGTGG AGCTGGCGCT GTGGGACACG 
GCCGGCCAGG AGGACTACGA CCGCCTGCGC CCTCTCTCCT ACCCAGACAC GGACGTGATC 
CTCATGTGCT TCTCAGTGGA CAGCCCGGAC TCGCTGGAGA ACATCCCGGA GAAGTGGGTG 
CCCGAAGTCA AGCACTTCTG CCCCAACGTC CCCATCATCC TGGTGGCCAA CAAGAAAGAC 
CTGCGCAACG ACGAGCACGT GCGTAACGAG CTGGCCCGCA TGAAGCAGGA GCCGGTGCGC 
ACTGAGGATG GCCGCGCCAT GGCCATCCGC ATCCAGGCCT ACGACTACCT GGAGTGCTCG 
GCCAAGACCA AGGAGGGTGT GCGGGAGGTC TTTGAGACGG CCACCCGGGC GGCCTTGCAG 
AAGCGCTACG GCACTCAGAA CGGCTGCATC AATTGCTGCA AGGTCCTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_990240.1
CDS	294..884
Translation	MAAIRKKLVVVGDGACGKTCLLIVFSKDEFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSVDSPDSLENIPEKWVPEVKHFCPNVPIILVANKKDLRNDEHVRNELARMKQEPVRTEDGRAMAIRIQAYDYLECSAKTKEGVREVFETATRAALQKRYGTQNGCINCCKVL

Target ORF information:

RefSeq Version	NM_204909.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ras homolog family member B (RHOB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204909.1

ORF Insert Sequence:

ATGGCCGCCA TCCGCAAGAA GCTGGTGGTG GTGGGAGACG GCGCCTGTGG CAAGACCTGC 
CTCCTCATCG TCTTCAGCAA GGACGAGTTC CCCGAGGTTT ACGTGCCCAC CGTCTTTGAG 
AACTACGTGG CCGACATCGA GGTGGACGGC AAGCAGGTGG AGCTGGCGCT GTGGGACACG 
GCCGGCCAGG AGGACTACGA CCGCCTGCGC CCTCTCTCCT ACCCAGACAC GGACGTGATC 
CTCATGTGCT TCTCAGTGGA CAGCCCGGAC TCGCTGGAGA ACATCCCGGA GAAGTGGGTG 
CCCGAAGTCA AGCACTTCTG CCCCAACGTC CCCATCATCC TGGTGGCCAA CAAGAAAGAC 
CTGCGCAACG ACGAGCACGT GCGTAACGAG CTGGCCCGCA TGAAGCAGGA GCCGGTGCGC 
ACTGAGGATG GCCGCGCCAT GGCCATCCGC ATCCAGGCCT ACGACTACCT GGAGTGCTCG 
GCCAAGACCA AGGAGGGTGT GCGGGAGGTC TTTGAGACGG CCACCCGGGC GGCCTTGCAG 
AAGCGCTACG GCACTCAGAA CGGCTGCATC AATTGCTGCA AGGTCCTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Small GTP-binding protein RhoB is expressed in glial M?ller cells in the vertebrate retina.
The Journal of comparative neurology494(6)976-85(2006 Feb)
Santos-Bredariol AS,Belmonte MA,Kihara AH,Santos MF,Hamassaki DE

LSox5 regulates RhoB expression in the neural tube and promotes generation of the neural crest.
Development (Cambridge, England)131(18)4455-65(2004 Sep)
Perez-Alcala S,Nieto MA,Barbas JA

Relative expression of Slug, RhoB, and HNK-1 in the cranial neural crest of the early chicken embryo.
Developmental dynamics : an official publication of the American Association of Anatomists229(1)136-9(2004 Jan)
Del Barrio MG,Nieto MA

Differential expression of distinct members of Rho family GTP-binding proteins during neuronal development: identification of Rac1B, a new neural-specific member of the family.
The Journal of neuroscience : the official journal of the Society for Neuroscience17(17)6717-28(1997 Sep)
Malosio ML,Gilardelli D,Paris S,Albertinazzi C,de Curtis I