GCLC cDNA ORF clone, Camelus ferus(Wild Bactrian camel)

Gene
Clones
gRNAs

Summary

Gene Symbol	GCLC
Entrez Gene ID	102516188
Full Name	glutamate-cysteine ligase catalytic subunit
Gene Type	protein-coding
Organism	Camelus ferus(Wild Bactrian camel)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006182367.2	XP_006182429.1	LOW QUALITY PROTEIN: glutamate--cysteine ligase catalytic subunit

Pathways from BioSystems
Gene Ontology

The following GCLC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GCLC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa42697	XM_006182367.2 Latest version!	Camelus ferus glutamate-cysteine ligase, catalytic subunit (GCLC), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GCLC ( XM_006182367.2 ) cDNA ORF clone, Camelus ferus(Wild Bactrian camel) -> XP_006182429.1 Camelus ferus glutamate-cysteine ligase, catalytic subunit (GCLC), mRNA.

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CloneID	OCa42697
Clone ID Related Accession (Same CDS sequence)	XM_006182367.2
Accession Version	XM_006182367.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-02
Organism	Camelus ferus(Wild Bactrian camel)
Product	LOW QUALITY PROTEIN: glutamate--cysteine ligase catalytic subunit
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006210778.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 3, 2015 this sequence version replaced XM_006182367.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Camelus ferus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## internal stop codons :: corrected 1 genomic stop codon ##RefSeq-Attributes-END##

ATGTTGGTGT CTTTCGATCA TGAAAACAAA AAAGTCCAAT TGGTCCTGTC TGGAGAGGAG 
GTTCTTGAAA CTCTGCAAGA AAAAGGGGAA AGGACAAACC CAAACCACCC TACCCTTTGG 
AGACCAGAGT ACGGGAGCTA CATGATCGAA GGGACACCGG GCCAGCCGTA CGGGGGGACC 
ATGTCTGAGT TCAACACCGT GGAGGACAAC ATGAGGAAGC GCCGCCAGGA GGCGACGTCT 
CTGCTGGGAG ACAACCAGGC TGTCTGCACG ATAACCTCAT TTCCCAGATT AGGCTGCCCT 
GGGTTCACGC TGCCCGAGTT CAGGCCCCGC CCGGTCGAAG GAGGAGCTTC CAAGTCCCTC 
TTCTTTCCCG ACGAAGCAAT CAACAAGCAC CCGCGCTTCA GCACCCTAAC AAGGAACATC 
CGGCACAGGA GGGGAGAGAA AGTCGTCATC AATGTCCCGA TATTTAAGGA CAAGAACACG 
CCGTCTCCGT TTATAGAGAC GTTTCCCGAG GATGACGAGG CAGCGAAGGC GGCTAAGCCG 
GACCACATTT ACATGGATGC CATGGGCTTC GGGATGGGCA ATTGCTGTCT CCAGGTAACG 
TTCCAAGCCT GCAGCATATC TGAGGCCCGC TACCTGTATG ATCAGTTGGC CACTATCTGC 
CCAATTGTTA TGGCTTTGAG CGCTGCGTCT CCTTTTTACC GAGGCTATGT GTCAGACATT 
GATTGTCGCT GGGGAGTGAT TTCTGCGTCT GTAGATGATA GAACTCGGGA GGAGAGAGGA 
CTGGAGCCGC TGAAGAACAA TAGCTATAGG ATTAGTAAAT CCCGGTACGA CTCCATCGAC 
AGTTACTTGT CCGAGTGCGG GGAGAAGTAC AATGACCTCG ACCTGACCAT AGACAGGGAG 
ATCTACGGCC ATCTGCTGCA GGAAGGCATC GATCACCTCC TGGCCCAGCA CGTGGCTCAC 
CTCTTCATCA GAGACCCGCT GACTCTGTTT GAGGAGAAAA TACACCTGGA CGATGCCAAC 
GAGTCTGACC ATTTCGAGAA TATTCAGTCC ACAAATTGGC AGACGATGAG ATTTAAGCCT 
CCTCCCCCAA ACTCCGACAT TGGATGGAGA GTGGAATTCC GGCCTATGGA GGTGCAGTTG 
ACAGACTTCG AGAACTCTGC ATACGTGGTG TTTGTGGTGC TGCTTACCAG GGTGATCCTG 
TCCTACAAAC TGGACTTCCT CATTCCCCTG TCGAAGGTGG ACGAGAACAT GAAAGTGGCA 
CAGAAGCGAG ACGCCGTCCT GCGGGGCAGG TTTTATTTCA GAAAAGACAT CTGCAAAGGT 
ACTGCGCTCT CTTGCATTTC TGGGGGCACC CGGGCCCGCG GCAGCCCCAA CCTCATAGCC 
TGCTTCCCAT AGGAAGGCGT GTTCCCTGGG CTGATCCCCA TCCTGAACTC TTACCTGGAG 
AACATGGAGG TGGATGTGGA CACCAGATGC AGTATTCTCA ACTACCTGAA GCTGATCAAG 
AAGAGAGCGT CCGGAGAATT AATGACAGTT GCCAGGTGGA TGAGGGAGTT CATCGCAAAG 
CATCCTGACT ACAAGCAAGA CAGTGTCATC ACTGACAAAA TAAACTATAG CCTTCTTTTA 
AAGTGTAACC AAATTGCAAA CGAATTATGT GAATGCCCAG AGTTACTTGG ACCAGCATTT 
AGGAAAGTAA AATATAGTGG AAGTAAAACC GACCCTTCCA ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006182429.1
CDS	7..1731
Translation	MLVSFDHENKKVQLVLSGEEVLETLQEKGERTNPNHPTLWRPEYGSYMIEGTPGQPYGGTMSEFNTVEDNMRKRRQEATSLLGDNQAVCTITSFPRLGCPGFTLPEFRPRPVEGGASKSLFFPDEAINKHPRFSTLTRNIRHRRGEKVVINVPIFKDKNTPSPFIETFPEDDEAAKAAKPDHIYMDAMGFGMGNCCLQVTFQACSISEARYLYDQLATICPIVMALSAASPFYRGYVSDIDCRWGVISASVDDRTREERGLEPLKNNSYRISKSRYDSIDSYLSECGEKYNDLDLTIDREIYGHLLQEGIDHLLAQHVAHLFIRDPLTLFEEKIHLDDANESDHFENIQSTNWQTMRFKPPPPNSDIGWRVEFRPMEVQLTDFENSAYVVFVVLLTRVILSYKLDFLIPLSKVDENMKVAQKRDAVLRGRFYFRKDICKGTALSCISGGTRARGSPNLIACFP*EGVFPGLIPILNSYLENMEVDVDTRCSILNYLKLIKKRASGELMTVARWMREFIAKHPDYKQDSVITDKINYSLLLKCNQIANELCECPELLGPAFRKVKYSGSKTDPSN

Target ORF information:

RefSeq Version	XM_006182367.2
Organism	Camelus ferus(Wild Bactrian camel)
Definition	Camelus ferus glutamate-cysteine ligase, catalytic subunit (GCLC), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006182367.2

ORF Insert Sequence:

ATGTTGGTGT CTTTCGATCA TGAAAACAAA AAAGTCCAAT TGGTCCTGTC TGGAGAGGAG 
GTTCTTGAAA CTCTGCAAGA AAAAGGGGAA AGGACAAACC CAAACCACCC TACCCTTTGG 
AGACCAGAGT ACGGGAGCTA CATGATCGAA GGGACACCGG GCCAGCCGTA CGGGGGGACC 
ATGTCTGAGT TCAACACCGT GGAGGACAAC ATGAGGAAGC GCCGCCAGGA GGCGACGTCT 
CTGCTGGGAG ACAACCAGGC TGTCTGCACG ATAACCTCAT TTCCCAGATT AGGCTGCCCT 
GGGTTCACGC TGCCCGAGTT CAGGCCCCGC CCGGTCGAAG GAGGAGCTTC CAAGTCCCTC 
TTCTTTCCCG ACGAAGCAAT CAACAAGCAC CCGCGCTTCA GCACCCTAAC AAGGAACATC 
CGGCACAGGA GGGGAGAGAA AGTCGTCATC AATGTCCCGA TATTTAAGGA CAAGAACACG 
CCGTCTCCGT TTATAGAGAC GTTTCCCGAG GATGACGAGG CAGCGAAGGC GGCTAAGCCG 
GACCACATTT ACATGGATGC CATGGGCTTC GGGATGGGCA ATTGCTGTCT CCAGGTAACG 
TTCCAAGCCT GCAGCATATC TGAGGCCCGC TACCTGTATG ATCAGTTGGC CACTATCTGC 
CCAATTGTTA TGGCTTTGAG CGCTGCGTCT CCTTTTTACC GAGGCTATGT GTCAGACATT 
GATTGTCGCT GGGGAGTGAT TTCTGCGTCT GTAGATGATA GAACTCGGGA GGAGAGAGGA 
CTGGAGCCGC TGAAGAACAA TAGCTATAGG ATTAGTAAAT CCCGGTACGA CTCCATCGAC 
AGTTACTTGT CCGAGTGCGG GGAGAAGTAC AATGACCTCG ACCTGACCAT AGACAGGGAG 
ATCTACGGCC ATCTGCTGCA GGAAGGCATC GATCACCTCC TGGCCCAGCA CGTGGCTCAC 
CTCTTCATCA GAGACCCGCT GACTCTGTTT GAGGAGAAAA TACACCTGGA CGATGCCAAC 
GAGTCTGACC ATTTCGAGAA TATTCAGTCC ACAAATTGGC AGACGATGAG ATTTAAGCCT 
CCTCCCCCAA ACTCCGACAT TGGATGGAGA GTGGAATTCC GGCCTATGGA GGTGCAGTTG 
ACAGACTTCG AGAACTCTGC ATACGTGGTG TTTGTGGTGC TGCTTACCAG GGTGATCCTG 
TCCTACAAAC TGGACTTCCT CATTCCCCTG TCGAAGGTGG ACGAGAACAT GAAAGTGGCA 
CAGAAGCGAG ACGCCGTCCT GCGGGGCAGG TTTTATTTCA GAAAAGACAT CTGCAAAGGT 
ACTGCGCTCT CTTGCATTTC TGGGGGCACC CGGGCCCGCG GCAGCCCCAA CCTCATAGCC 
TGCTTCCCAT AGGAAGGCGT GTTCCCTGGG CTGATCCCCA TCCTGAACTC TTACCTGGAG 
AACATGGAGG TGGATGTGGA CACCAGATGC AGTATTCTCA ACTACCTGAA GCTGATCAAG 
AAGAGAGCGT CCGGAGAATT AATGACAGTT GCCAGGTGGA TGAGGGAGTT CATCGCAAAG 
CATCCTGACT ACAAGCAAGA CAGTGTCATC ACTGACAAAA TAAACTATAG CCTTCTTTTA 
AAGTGTAACC AAATTGCAAA CGAATTATGT GAATGCCCAG AGTTACTTGG ACCAGCATTT 
AGGAAAGTAA AATATAGTGG AAGTAAAACC GACCCTTCCA ACTAG