VWA5B1 cDNA ORF clone, Calypte anna(Anna's hummingbird)

Gene
Clones
gRNAs

Summary

Gene Symbol	VWA5B1
Entrez Gene ID	103526211
Full Name	von Willebrand factor A domain containing 5B1
Gene Type	protein-coding
Organism	Calypte anna(Anna's hummingbird)

mRNA and Protein(s)

mRNA	Protein	Name
XM_030463432.1	XP_030319292.1	LOW QUALITY PROTEIN: von Willebrand factor A domain-containing protein 5B1
XM_008491241.1	XP_008489463.1	LOW QUALITY PROTEIN: von Willebrand factor A domain-containing protein 5B1

Pathways from BioSystems
Gene Ontology

The following VWA5B1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VWA5B1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa418444	XM_008491241.1 Latest version!	Calypte anna von Willebrand factor A domain containing 5B1 (VWA5B1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa815300	XM_030463432.1 Latest version!	Calypte anna von Willebrand factor A domain containing 5B1 (VWA5B1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VWA5B1 ( XM_008491241.1 ) cDNA ORF clone, Calypte anna(Anna's hummingbird) -> XP_008489463.1 Calypte anna von Willebrand factor A domain containing 5B1 (VWA5B1), mRNA.

Price & Availability↑

CloneID	OCa418444
Clone ID Related Accession (Same CDS sequence)	XM_008491241.1
Accession Version	XM_008491241.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-07-09
Organism	Calypte anna(Anna's hummingbird)
Product	LOW QUALITY PROTEIN: von Willebrand factor A domain-containing protein 5B1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_007619454.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calypte anna Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 28% of CDS bases internal stop codons :: corrected 1 genomic stop codon ##RefSeq-Attributes-END##

ATGCCAGGGC TTATTAACCA GACCACCCTC TCCTTGGTCC CCCTCACTGC CTCAGAGGTG 
ACGTCTTGCA TCCGTGGCTA TGCCTTTGGG ATGACAGCCT TGCTCACCTA CTGCAACCCA 
GAAGCCAAGC TTTTGAAGGC AAGTCTCTTC ATCTACCCCT TGGATGAGGG CACCACTGTC 
GTGGGGTTCG AGGCGGCCAT GTCCCGACGT GCTATCACGG TGCAGATCAA AGACAAAGCC 
AAGATGGATG ATGAGCATTT CCCACAGTTG TGACACCACA TCTCTCTGGT GTGGTCATCT 
CTCCATACAG GAAGGATCAT GATGGATGAG GACTTGGAGA GGACTGTTTT TGTGGCTAAC 
CTGGGCATGA TTCCTCCCCT GGAAAGCATC TCAATCTTCA TCAGCACCTC TTCTGAGCTG 
CAGACACTGC CAAGTGGGGC TATGAGGGTC ATTCTGCCAG CTGTGTGTGT CCCCAGGATC 
CTCCAGCCCA GCCTGGAGAA CACCAGCAGT TTTTCCAGCC ACCAGCTCCC AATCAACAAA 
CACCACTGGG GATCAGGGAG CCCAGAGAAA GGGAGCAGAT TCTGCCTGGC TCAGCTGCTG 
GAGAACGAGG CTACCAACCC CTTTGAGTAC GAGTTCAGCT TCCACCTGGA GATCCGTGGA 
CCCTGTCTGC TGGCAGGAGT TGAGAGCCCC ACACATGAGC TCCGAGCAGA TGCTGACCCC 
TCTGCTCACT CTGCCAAGAG CATCATCATC ACCCTGGCCA ACAAACACAC CTTTGACAGG 
CCCGTGGAGA TCTTGATCCA CCCAAGTGAA CCCCACATGC CTCACATCTT AATGGAAGAA 
GGTGACATGA CCCCTGCAGA GTATGAGCAC CACCTGAAGG GAAAGAGTGA CTTCATTAAA 
GCCACTAAGA AGGACCCCAG CACTGAGAAA AAGACAGAAA TCATCTGGAA GCGGCTGAAC 
AAGGACATCC CCCACCACCC TGTCATCATG CTTAACTTCT GCCCTGACTT GATGACCTTC 
CAGCCAGACC TCCAGAAAGC CCAAGGAGAA TTTATCTTCC TGGTAGACCG CAGCAGGAGC 
ATGAGTGGCA TGAACATCAA CCGTGTCAAG GATGCTCTGT TGGTCATCCT TAAGAGCCTG 
ATGCCAGCGT GTCTCTTCAA CATCGTTGGG TTTGGCTCTA CCTTCAAGAC CCTCTTTCCT 
TCCAGCCAGG CTTACTGTGA GGAGAGCTTG GCCATCGCTT GCGAGAGCAT CAAGAAGATC 
CGAGCTGACA TGGGTGGCAC CAACGTCTTG TCACCATTGA GGTGGGTTGT TCGACAGCCC 
ATCCACAGGG GCCACGCCCG CCTGCTCTTT CTGCTGATGA ACGGGGCCAT CAGCAACACG 
GGGAAGGTCC TGGAGCTGCT GCAGAACCAC TCCTGCTCCA CCAGGTGCTA CAGCTTTGGC 
ATTGGGCCCA ATGCCTGCAG GAGACTGGTT GGGGGGCTGG CTGGTGTCTC CAGGGGCATC 
ACTGAGTTTC CTGGGGAAGG CGAGAGATTG CAGCCCAAGA TGATCAAATC TCTGAAGAAA 
GCAATGGCAC CAGTCCTGAG TGATGTGTCT GTGGAGTGGA TCTTCCCTGA GAGCACTGAG 
GTCTTGGTTT CTCCCATCAG CACCAGTTGC CTCTTCCCTG GTGACCACCT TGTCAGCTAC 
AGCATCATCT GTGACACCTC CCCATACATC TCCAATCCCC AGTCTGACAA GAGGCGACAT 
TACAGCATGA TGAGGTCCCA GGAGTCGGGC AGCTCTGTTT TCTTTCACTC CCAGGAGGAG 
GGAGCAGACG GGGAGAGCTG GAACCACTCC AGAGATGGGG AAAGCTACTG TCCTGCTGAG 
CATGGCCCCC ATCACCTGGT GGTTGGCAGA GAACCTGGGG GAGAATCAGA CACAGACACT 
CGAATGGAGG TGAAGGCTTT CTCCAGGAGA AGGGCTTACA GCACAACCCA AATCATTGAC 
CACAAGCCTT TCAAGAAGGT GCCCACAGCC AGCGATCCTG GCACTGACCT GGTGAAAAAT 
CCCCTCCGGA AAACCCAGCC ACAGGACCTG CAGCAGCTCA GCCCTGAGCC CTGGCAGGTG 
GATATCCAGC CTGGTGTTGC TATCCCCCTT GATGGGAGGG CTCCCAGCAG CTCTCCCAGC 
GCCCAGGCCA GCCTCCACCC CTCCAGGGCT GTGAGCCCTC AGCTCATCTT CACACTCACT 
GTGGTGGGAT GGAAAGACTC TGCCCAGCAT CCTCCCTTCA CATTTGAAAC AGAGACCTCC 
TCTGACTGGG AACCCCAGGA CTTAGACTTC AGTGCTTCCC ATGGCTCCTC CTGTTCTCCC 
AGGACCTTCT GCAAGGTGAT GGTGAAAGGG CTGAGGAACA ACGAACCTGT GCAGTGGGAG 
GTCACCTTTG ATATCCACCC ACTTTTCCAA GAGCAAGAGA GCCAGGCAGA TGGTGACACA 
GACCTGTGGA ATGAGACCTT CCACCACCTG GCAGCCAAGT CAGTCATCCG GGACTTTGAG 
CAGCTCGCTG AGAAGGAGTG TGACATCGAG CATGGGTCTG GGCGCAGGTA CCAGATCAAC 
ACTGTCCACA CCAGCAAGGC CTGCAACATC ATCAGCAAGT ACACAATGTT CATGCCAGTG 
GACCTGAGCA CCAACACCTA CCTGCCTACT GTTGTTGAGT ACACCCACAC AGGGCCATTA 
CAGAAGCTGC CTACTGCCTC TGCAGAAGTA CCAGAATCCA TTGAGAGCCT CTGTGCTGCC 
AGGCTGACCT TCAATAAAAC CAGGCTGCTG ACTCAAGCTG CCAAAAGGTT CATGAGTAAA 
TATCCAAGCA GAGTGGGTGA AGCCAGCTCT GAGGGCGAGA ATGAGAAGAT GGACTACCTT 
CCCCTGGCAT CTCTACAGCT GGCCTGTGGT GCCTTCCTCA TGAACTCAGC CTTCTCTGCT 
GCTGTCAACA TCCCCATGGA GAAGCTGAAG TGGACCTCTC CCTTCGCCTG CCACCGCTTG 
GCCCTCAGCC CCTCCAGCTC CTACAGCACC AAGAGGACCA ACCCCTCCGT GGAGAAAGAA 
AGTTTCAGCT CCAGACTGCA GTTCCTGGTT CCTGATGACC ACAGGAAGAG TCCCACCACC 
AGAGACATCC CCCTAGGAGC AGTGCCAGAG GGAGCTGGCA GCCATCCTGA GGACACTGCC 
CACCTTCGCC ACTTCTCAGG CAGCACGGTG GAAAGCATCT CCAAAGCCCT GAAAGATGAG 
AAGGAACTGT CCCCACCAAC CATTGCCATC CGCCACTTCT CCAGCCCAGA GAGCACCTTG 
GCCTCAACCC ACCAGCAAAC CAACTGTGGT GAGGGCTTGG AGATGGATGG GTCTGAAGTC 
CAAGAGTTGC TCTTTGACAT TGAGCTGCAG CAGCAGACAG AACCAGAGGG GATGCTCTGG 
GCCAAGGCAG TGGCCCTGGC CTGGCTGGAG CATAGCTCAG CTTCTTATTT CATCGAGTGG 
GAGCTGGTGG CAGCCAAGGC CAACCTGTGG CTGAGCCAAC AGGACTTCCC CGAGGGATAC 
AGCTTGGCCA CTGTGAAAGC TGCAGCCCAG CAGCTCTTTG ACCTGCTCCG GCACTGGGAT 
GAGAACCTGG AGTTCAACCT GCTGTGCTAC AACCCCAACA GCGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008489463.1
CDS	1..3648
Translation	MPGLINQTTLSLVPLTASEVTSCIRGYAFGMTALLTYCNPEAKLLKASLFIYPLDEGTTVVGFEAAMSRRAITVQIKDKAKMDDEHFPQL*HHISLVWSSLHTGRIMMDEDLERTVFVANLGMIPPLESISIFISTSSELQTLPSGAMRVILPAVCVPRILQPSLENTSSFSSHQLPINKHHWGSGSPEKGSRFCLAQLLENEATNPFEYEFSFHLEIRGPCLLAGVESPTHELRADADPSAHSAKSIIITLANKHTFDRPVEILIHPSEPHMPHILMEEGDMTPAEYEHHLKGKSDFIKATKKDPSTEKKTEIIWKRLNKDIPHHPVIMLNFCPDLMTFQPDLQKAQGEFIFLVDRSRSMSGMNINRVKDALLVILKSLMPACLFNIVGFGSTFKTLFPSSQAYCEESLAIACESIKKIRADMGGTNVLSPLRWVVRQPIHRGHARLLFLLMNGAISNTGKVLELLQNHSCSTRCYSFGIGPNACRRLVGGLAGVSRGITEFPGEGERLQPKMIKSLKKAMAPVLSDVSVEWIFPESTEVLVSPISTSCLFPGDHLVSYSIICDTSPYISNPQSDKRRHYSMMRSQESGSSVFFHSQEEGADGESWNHSRDGESYCPAEHGPHHLVVGREPGGESDTDTRMEVKAFSRRRAYSTTQIIDHKPFKKVPTASDPGTDLVKNPLRKTQPQDLQQLSPEPWQVDIQPGVAIPLDGRAPSSSPSAQASLHPSRAVSPQLIFTLTVVGWKDSAQHPPFTFETETSSDWEPQDLDFSASHGSSCSPRTFCKVMVKGLRNNEPVQWEVTFDIHPLFQEQESQADGDTDLWNETFHHLAAKSVIRDFEQLAEKECDIEHGSGRRYQINTVHTSKACNIISKYTMFMPVDLSTNTYLPTVVEYTHTGPLQKLPTASAEVPESIESLCAARLTFNKTRLLTQAAKRFMSKYPSRVGEASSEGENEKMDYLPLASLQLACGAFLMNSAFSAAVNIPMEKLKWTSPFACHRLALSPSSSYSTKRTNPSVEKESFSSRLQFLVPDDHRKSPTTRDIPLGAVPEGAGSHPEDTAHLRHFSGSTVESISKALKDEKELSPPTIAIRHFSSPESTLASTHQQTNCGEGLEMDGSEVQELLFDIELQQQTEPEGMLWAKAVALAWLEHSSASYFIEWELVAAKANLWLSQQDFPEGYSLATVKAAAQQLFDLLRHWDENLEFNLLCYNPNSV

Target ORF information:

RefSeq Version	XM_008491241.1
Organism	Calypte anna(Anna's hummingbird)
Definition	Calypte anna von Willebrand factor A domain containing 5B1 (VWA5B1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008491241.1

ORF Insert Sequence:

ATGCCAGGGC TTATTAACCA GACCACCCTC TCCTTGGTCC CCCTCACTGC CTCAGAGGTG 
ACGTCTTGCA TCCGTGGCTA TGCCTTTGGG ATGACAGCCT TGCTCACCTA CTGCAACCCA 
GAAGCCAAGC TTTTGAAGGC AAGTCTCTTC ATCTACCCCT TGGATGAGGG CACCACTGTC 
GTGGGGTTCG AGGCGGCCAT GTCCCGACGT GCTATCACGG TGCAGATCAA AGACAAAGCC 
AAGATGGATG ATGAGCATTT CCCACAGTTG TGACACCACA TCTCTCTGGT GTGGTCATCT 
CTCCATACAG GAAGGATCAT GATGGATGAG GACTTGGAGA GGACTGTTTT TGTGGCTAAC 
CTGGGCATGA TTCCTCCCCT GGAAAGCATC TCAATCTTCA TCAGCACCTC TTCTGAGCTG 
CAGACACTGC CAAGTGGGGC TATGAGGGTC ATTCTGCCAG CTGTGTGTGT CCCCAGGATC 
CTCCAGCCCA GCCTGGAGAA CACCAGCAGT TTTTCCAGCC ACCAGCTCCC AATCAACAAA 
CACCACTGGG GATCAGGGAG CCCAGAGAAA GGGAGCAGAT TCTGCCTGGC TCAGCTGCTG 
GAGAACGAGG CTACCAACCC CTTTGAGTAC GAGTTCAGCT TCCACCTGGA GATCCGTGGA 
CCCTGTCTGC TGGCAGGAGT TGAGAGCCCC ACACATGAGC TCCGAGCAGA TGCTGACCCC 
TCTGCTCACT CTGCCAAGAG CATCATCATC ACCCTGGCCA ACAAACACAC CTTTGACAGG 
CCCGTGGAGA TCTTGATCCA CCCAAGTGAA CCCCACATGC CTCACATCTT AATGGAAGAA 
GGTGACATGA CCCCTGCAGA GTATGAGCAC CACCTGAAGG GAAAGAGTGA CTTCATTAAA 
GCCACTAAGA AGGACCCCAG CACTGAGAAA AAGACAGAAA TCATCTGGAA GCGGCTGAAC 
AAGGACATCC CCCACCACCC TGTCATCATG CTTAACTTCT GCCCTGACTT GATGACCTTC 
CAGCCAGACC TCCAGAAAGC CCAAGGAGAA TTTATCTTCC TGGTAGACCG CAGCAGGAGC 
ATGAGTGGCA TGAACATCAA CCGTGTCAAG GATGCTCTGT TGGTCATCCT TAAGAGCCTG 
ATGCCAGCGT GTCTCTTCAA CATCGTTGGG TTTGGCTCTA CCTTCAAGAC CCTCTTTCCT 
TCCAGCCAGG CTTACTGTGA GGAGAGCTTG GCCATCGCTT GCGAGAGCAT CAAGAAGATC 
CGAGCTGACA TGGGTGGCAC CAACGTCTTG TCACCATTGA GGTGGGTTGT TCGACAGCCC 
ATCCACAGGG GCCACGCCCG CCTGCTCTTT CTGCTGATGA ACGGGGCCAT CAGCAACACG 
GGGAAGGTCC TGGAGCTGCT GCAGAACCAC TCCTGCTCCA CCAGGTGCTA CAGCTTTGGC 
ATTGGGCCCA ATGCCTGCAG GAGACTGGTT GGGGGGCTGG CTGGTGTCTC CAGGGGCATC 
ACTGAGTTTC CTGGGGAAGG CGAGAGATTG CAGCCCAAGA TGATCAAATC TCTGAAGAAA 
GCAATGGCAC CAGTCCTGAG TGATGTGTCT GTGGAGTGGA TCTTCCCTGA GAGCACTGAG 
GTCTTGGTTT CTCCCATCAG CACCAGTTGC CTCTTCCCTG GTGACCACCT TGTCAGCTAC 
AGCATCATCT GTGACACCTC CCCATACATC TCCAATCCCC AGTCTGACAA GAGGCGACAT 
TACAGCATGA TGAGGTCCCA GGAGTCGGGC AGCTCTGTTT TCTTTCACTC CCAGGAGGAG 
GGAGCAGACG GGGAGAGCTG GAACCACTCC AGAGATGGGG AAAGCTACTG TCCTGCTGAG 
CATGGCCCCC ATCACCTGGT GGTTGGCAGA GAACCTGGGG GAGAATCAGA CACAGACACT 
CGAATGGAGG TGAAGGCTTT CTCCAGGAGA AGGGCTTACA GCACAACCCA AATCATTGAC 
CACAAGCCTT TCAAGAAGGT GCCCACAGCC AGCGATCCTG GCACTGACCT GGTGAAAAAT 
CCCCTCCGGA AAACCCAGCC ACAGGACCTG CAGCAGCTCA GCCCTGAGCC CTGGCAGGTG 
GATATCCAGC CTGGTGTTGC TATCCCCCTT GATGGGAGGG CTCCCAGCAG CTCTCCCAGC 
GCCCAGGCCA GCCTCCACCC CTCCAGGGCT GTGAGCCCTC AGCTCATCTT CACACTCACT 
GTGGTGGGAT GGAAAGACTC TGCCCAGCAT CCTCCCTTCA CATTTGAAAC AGAGACCTCC 
TCTGACTGGG AACCCCAGGA CTTAGACTTC AGTGCTTCCC ATGGCTCCTC CTGTTCTCCC 
AGGACCTTCT GCAAGGTGAT GGTGAAAGGG CTGAGGAACA ACGAACCTGT GCAGTGGGAG 
GTCACCTTTG ATATCCACCC ACTTTTCCAA GAGCAAGAGA GCCAGGCAGA TGGTGACACA 
GACCTGTGGA ATGAGACCTT CCACCACCTG GCAGCCAAGT CAGTCATCCG GGACTTTGAG 
CAGCTCGCTG AGAAGGAGTG TGACATCGAG CATGGGTCTG GGCGCAGGTA CCAGATCAAC 
ACTGTCCACA CCAGCAAGGC CTGCAACATC ATCAGCAAGT ACACAATGTT CATGCCAGTG 
GACCTGAGCA CCAACACCTA CCTGCCTACT GTTGTTGAGT ACACCCACAC AGGGCCATTA 
CAGAAGCTGC CTACTGCCTC TGCAGAAGTA CCAGAATCCA TTGAGAGCCT CTGTGCTGCC 
AGGCTGACCT TCAATAAAAC CAGGCTGCTG ACTCAAGCTG CCAAAAGGTT CATGAGTAAA 
TATCCAAGCA GAGTGGGTGA AGCCAGCTCT GAGGGCGAGA ATGAGAAGAT GGACTACCTT 
CCCCTGGCAT CTCTACAGCT GGCCTGTGGT GCCTTCCTCA TGAACTCAGC CTTCTCTGCT 
GCTGTCAACA TCCCCATGGA GAAGCTGAAG TGGACCTCTC CCTTCGCCTG CCACCGCTTG 
GCCCTCAGCC CCTCCAGCTC CTACAGCACC AAGAGGACCA ACCCCTCCGT GGAGAAAGAA 
AGTTTCAGCT CCAGACTGCA GTTCCTGGTT CCTGATGACC ACAGGAAGAG TCCCACCACC 
AGAGACATCC CCCTAGGAGC AGTGCCAGAG GGAGCTGGCA GCCATCCTGA GGACACTGCC 
CACCTTCGCC ACTTCTCAGG CAGCACGGTG GAAAGCATCT CCAAAGCCCT GAAAGATGAG 
AAGGAACTGT CCCCACCAAC CATTGCCATC CGCCACTTCT CCAGCCCAGA GAGCACCTTG 
GCCTCAACCC ACCAGCAAAC CAACTGTGGT GAGGGCTTGG AGATGGATGG GTCTGAAGTC 
CAAGAGTTGC TCTTTGACAT TGAGCTGCAG CAGCAGACAG AACCAGAGGG GATGCTCTGG 
GCCAAGGCAG TGGCCCTGGC CTGGCTGGAG CATAGCTCAG CTTCTTATTT CATCGAGTGG 
GAGCTGGTGG CAGCCAAGGC CAACCTGTGG CTGAGCCAAC AGGACTTCCC CGAGGGATAC 
AGCTTGGCCA CTGTGAAAGC TGCAGCCCAG CAGCTCTTTG ACCTGCTCCG GCACTGGGAT 
GAGAACCTGG AGTTCAACCT GCTGTGCTAC AACCCCAACA GCGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VWA5B1 ( XM_030463432.1 ) cDNA ORF clone, Calypte anna(Anna's hummingbird) -> XP_030319292.1 Calypte anna von Willebrand factor A domain containing 5B1 (VWA5B1), mRNA.

Price & Availability↑

CloneID	OCa815300
Clone ID Related Accession (Same CDS sequence)	XM_030463432.1
Accession Version	XM_030463432.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-19
Organism	Calypte anna(Anna's hummingbird)
Product	LOW QUALITY PROTEIN: von Willebrand factor A domain-containing protein 5B1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044266.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Calypte anna Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 29% of CDS bases internal stop codons :: corrected 1 genomic stop codon ##RefSeq-Attributes-END##

ATGCCAGGGC TTATTAACCA GACCACCCTC TCCTCGGTCC CCCTCACTGC CTCAGAGGTG 
ACGTCTTGCA TCCGTGGCTA TGCCTTTGGG ATGACAGCCT TGCTCACCTA CTGCAACCCA 
GAAGCCAAGC TTTTGAAGGC AAGTCTCTTC ATCTACCCCT TGGATGAGGG CACCACTGTC 
GTGGGGTTCG AGGCGGCCAT GTCCCGACGT GCTATCACGG TGCAGATCAA AGACAAAGCC 
AAGATGGATG ATGAGCATTT CCCACAGTTG TGACACCACA TCTCTCTGGT GTGGTCATCT 
CTCCATACAG GAAGGATCAT GATGGATGAG GACTTGGAGA GGACTGTTTT TGTGGCTAAC 
CTGGGCATGA TTCCTCCTCT GGAAAGCATC TCAATCTTCA TAAGCACCTC TTCTGAGCTG 
CAGACACTGC CAAGTGGGGC TATGAGGGTC ATTCTGCCAG CTGTGTGTGT CCCCAGGATC 
CTCCAGCCCA GCCTGGAGAA CACCAGCAGT TTTTCCAGCC ACCAGCTCCC AATCAACAAA 
CACCACTGGG GATCAGGGAG CCCAGAGAAA GGGAGCAGAT TCTGCCTGGC TCAGCTGCTG 
GAGAACGAGG CTACCAACCC CTTTGAGTAC GAGTTCAGCT TCCACCTGGA GATCCGTGGA 
CCCTGTCTGC TGGCAGGAGT TGAGAGCCCC ACACATGAGC TCCGAGCAGA TGCTGACCCC 
TCTGCTCACT CTGCCAAGAG CATCATCATC ACCCTGGCCA ACAAACACAC CTTTGACAGG 
CCCGTGGAGA TCTTGATCCA CCCAAGTGAA CCCCACATGC CTCACATCTT AATGGAAGAA 
GGTGACATGA CCCCTGCAGA GTATGAGCAC CACCTGAAGG GAAAGAGTGA CTTCATTAAA 
GCCACTAAGA AGGACCCCAG CACTGAGAAA AAGACAGAAA TCATCTGGAA GCGGCTGAAC 
AAGGACATCC CCCACCACCC TGTCATCATG CTTAACTTCT GCCCTGACTT GATGACCTTC 
CAGCCAGACC TCCAGAAAGC CCAAGGAGAA TTTATCTTCC TGGTAGACCG CAGCAGGAGC 
ATGAGTGGCA TGAACATCAA CCGTGTCAAG GATGCTCTGT TGGTCATCCT TAAGAGCCTG 
ATGCCAGCGT GTCTCTTCAA CATCGTTGGG TTTGGCTCTA CCTTCAAGAC CCTCTTTCCT 
TCCAGCCAGG CTTACTGTGA GGAGAGCTTG GCCATCGCTT GCGAGAGCAT CAAGAAGATC 
CGAGCTGACA TGGGTGGCAC CAACGTCTTG TCACCATTGA GGTGGGTTGT TCGACAGCCC 
ATCCACAGGG GCCACCCCCG CTTGCTCTTT CTGCTGATGA ACGGGGCCAT CAGCAACACG 
GGGAAGGTCC TGGAGCTGCT GCAGAACCAC TCCTGCTCCA CCAGGTGCTA CAGCTTTGGC 
ATTGGGCCCA ATGCCTGCAG GAGACTGGTT GGGGGGCTGG CTGGTGTCTC CAGGGGCATC 
GCTGAGTTCC TGGTGGAAGG CGAGAGATTG CAGCCCAAGA TGATCAAATC TCTGAAGAAA 
GCAATGGCAC CAGTCCTGAG TGATGTGTCT GTGGAGTGGA TCTTCCCTGA GAGCACTGAG 
GTCTTGGTTT CTCCCATCAG CACCAGTTGC CTCTTCCCTG GTGACCACCT TGTCAGCTAC 
AGCATCATCT GTGACACCTC CCCATACATC TCCAATCCCC AGTCTGACAA GAGGCGACAT 
TACAGCATGA TGAGGTCCCA GGAGTCGGGC AGCTCTGTTT TCTTTCACTC CCAGGAGGAG 
GGAGCAGACG GGGAGAGCTG GAACCACTCC AGAGATGGGG AAAGCTACTG TCCTGCTGAG 
CATGGCCCCC ATCACCTGGT GGTTGGCAGA GAACCTGGGG GAGAATCAGA CACAGACACT 
CGAATGGAGG TGAAGGCTTT CTCCAGGAGA AGGGCTTACA GCACAACCCA AATCATTGAC 
CACAAGCCTT TCAAGAAGGT GCCCACAGCC AGCGATCCTG GCACTGACCT GGTGAAAAAT 
CCCCTCCGGA AAACCCAGCC ACAGGACCTG CAGCAGCTCA GCCCTGAGCC CTGGCAGGTG 
GATATCCAGC CCTTGCCAGC CACCTCACTT GGCTCTGCCA CCAAGATCCA TGGTGCTAGT 
GCCTGGAGGC CATCCCTGCT CCACCACAGC TGTGTGTCCT TCTGCCACCA CACCAAGTCC 
CCCGTGGCAC CTCCAAGAAG GGTCTTGGGA CCACTGGATG CTAGCACTGG CCTGAGGTCT 
TCATCGGACA GCGGAAGCCC CAGGGAGCTG GACTCTGCCC AGCATCCTCC CTTCACATTT 
GAAACAGAGA CCTCCTCTGA CTGGGAACCC CAGGACTTAG ACTTCAGTGC TTCCCATGGC 
TCCTCCTGTT CTCCCAGGAC CTTCTGCAAG GTGATGGTGA AAGGGCTGAG GAACAACGAA 
CCTGTGCAGT GGGAGGTCAC CTTTGATATC CACCCACTTT TCCAAGAGCA AGAGAGCCAG 
GCAGATGGTG ACACAGACCT GTGGAATGAG ACCTTCCACC ACCTGGCAGC CAAGTCAGTC 
ATCCGGGACT TTGAGCAGCT CGCTGAGAAG GAGTGTGACA TCGAGCATGG GTCTGGGCGC 
AGGTACCAGA TCAACACTGT CCACACCAGC AAGGCCTGCA ACATCATCAG CAAGTACACA 
ATGTTCATGC CAGTGGACCT GAGCACCAAC ACCTACCTGC CTACTGTTGT TGAGTACACC 
CACACAGGGC CATTACAGAA GCTGCCTACT GCCTCTGCAG AAGTACCAGA ATCCATTGAG 
AGCCTCTGTG CTGCCAGGCT GACCTTCAAT AAAACCAGGC TGCTGACTCA AGCTGCCAAA 
AGGTTCATGA GTAAATATCC AAGCAGAGTG GGTGAAGCCA GCTCTGAGGG CGAGAATGAG 
AAGATGGACT ACCTTCCCCT GGCATCTCTA CAGCTGGCCT GTGGTGCCTT CCTCATGAAC 
TCAGCCTTCT CTGCTGCTGT CAACATCCCC ATGGAGAAGC TGAAGTGGAC CTCTCCCTTC 
GCCTGCCACC GCTTGGCCCT CAGCCCCTCC AGCTCCTACA GCACCAAGAG GACCAACCCC 
TCCGTGGAGA AAGAAAGCCT CAGCTCCAGA CTGCAGTTCC TGGTTCCTGA TGACCACAGG 
AAGAGTCCCA CCACCAGAGA CATCCCCCTA GGAGCAGTGC CAGAGGGAGC TGGCAGCCAT 
CCTGAGGACA CTGCCCACCT TCGCCACTTC TCAGGCAGCA CGGTGGAAAG CATCTCCAAA 
GCCCTGAAAG ATGAGAAGGA ACTGTCCCCA CCAACCATTG CCATCCGCCC AGAGAGCACC 
TTGGCCTCAA CCCACCAGCA AACCAACTGT GGTGAGGGCT TGGAGATGGA TGGGTCTGAA 
GTCCAAGAGT TGCTCTTTGA CATTGAGCTG CAGCAGCAGA CAGAACCAGA GGGGATGCTC 
TGGGCCAAGG CAGTGGCCCT GGCCTGGCTG GAGCATAGCT CAGCTTCTTA TTTCATCGAG 
TGGGAGCTGG TGGCAGCCAA GGCCAACCTG TGGCTGAGCC AACAGGACTT CCCCGAGGGA 
TACAGCTTGG CCACTGTGAA AGCTGCAGCC CAGCAGCTCT TTGACCTGCT CCGGCACTGG 
GATGAGAACC TGGAGTTCAA CCTGCTGTGC TACAACCCCA ACAGCGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030319292.1
CDS	1..3711
Translation	MPGLINQTTLSSVPLTASEVTSCIRGYAFGMTALLTYCNPEAKLLKASLFIYPLDEGTTVVGFEAAMSRRAITVQIKDKAKMDDEHFPQL*HHISLVWSSLHTGRIMMDEDLERTVFVANLGMIPPLESISIFISTSSELQTLPSGAMRVILPAVCVPRILQPSLENTSSFSSHQLPINKHHWGSGSPEKGSRFCLAQLLENEATNPFEYEFSFHLEIRGPCLLAGVESPTHELRADADPSAHSAKSIIITLANKHTFDRPVEILIHPSEPHMPHILMEEGDMTPAEYEHHLKGKSDFIKATKKDPSTEKKTEIIWKRLNKDIPHHPVIMLNFCPDLMTFQPDLQKAQGEFIFLVDRSRSMSGMNINRVKDALLVILKSLMPACLFNIVGFGSTFKTLFPSSQAYCEESLAIACESIKKIRADMGGTNVLSPLRWVVRQPIHRGHPRLLFLLMNGAISNTGKVLELLQNHSCSTRCYSFGIGPNACRRLVGGLAGVSRGIAEFLVEGERLQPKMIKSLKKAMAPVLSDVSVEWIFPESTEVLVSPISTSCLFPGDHLVSYSIICDTSPYISNPQSDKRRHYSMMRSQESGSSVFFHSQEEGADGESWNHSRDGESYCPAEHGPHHLVVGREPGGESDTDTRMEVKAFSRRRAYSTTQIIDHKPFKKVPTASDPGTDLVKNPLRKTQPQDLQQLSPEPWQVDIQPLPATSLGSATKIHGASAWRPSLLHHSCVSFCHHTKSPVAPPRRVLGPLDASTGLRSSSDSGSPRELDSAQHPPFTFETETSSDWEPQDLDFSASHGSSCSPRTFCKVMVKGLRNNEPVQWEVTFDIHPLFQEQESQADGDTDLWNETFHHLAAKSVIRDFEQLAEKECDIEHGSGRRYQINTVHTSKACNIISKYTMFMPVDLSTNTYLPTVVEYTHTGPLQKLPTASAEVPESIESLCAARLTFNKTRLLTQAAKRFMSKYPSRVGEASSEGENEKMDYLPLASLQLACGAFLMNSAFSAAVNIPMEKLKWTSPFACHRLALSPSSSYSTKRTNPSVEKESLSSRLQFLVPDDHRKSPTTRDIPLGAVPEGAGSHPEDTAHLRHFSGSTVESISKALKDEKELSPPTIAIRPESTLASTHQQTNCGEGLEMDGSEVQELLFDIELQQQTEPEGMLWAKAVALAWLEHSSASYFIEWELVAAKANLWLSQQDFPEGYSLATVKAAAQQLFDLLRHWDENLEFNLLCYNPNSV

Target ORF information:

RefSeq Version	XM_030463432.1
Organism	Calypte anna(Anna's hummingbird)
Definition	Calypte anna von Willebrand factor A domain containing 5B1 (VWA5B1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030463432.1

ORF Insert Sequence:

ATGCCAGGGC TTATTAACCA GACCACCCTC TCCTCGGTCC CCCTCACTGC CTCAGAGGTG 
ACGTCTTGCA TCCGTGGCTA TGCCTTTGGG ATGACAGCCT TGCTCACCTA CTGCAACCCA 
GAAGCCAAGC TTTTGAAGGC AAGTCTCTTC ATCTACCCCT TGGATGAGGG CACCACTGTC 
GTGGGGTTCG AGGCGGCCAT GTCCCGACGT GCTATCACGG TGCAGATCAA AGACAAAGCC 
AAGATGGATG ATGAGCATTT CCCACAGTTG TGACACCACA TCTCTCTGGT GTGGTCATCT 
CTCCATACAG GAAGGATCAT GATGGATGAG GACTTGGAGA GGACTGTTTT TGTGGCTAAC 
CTGGGCATGA TTCCTCCTCT GGAAAGCATC TCAATCTTCA TAAGCACCTC TTCTGAGCTG 
CAGACACTGC CAAGTGGGGC TATGAGGGTC ATTCTGCCAG CTGTGTGTGT CCCCAGGATC 
CTCCAGCCCA GCCTGGAGAA CACCAGCAGT TTTTCCAGCC ACCAGCTCCC AATCAACAAA 
CACCACTGGG GATCAGGGAG CCCAGAGAAA GGGAGCAGAT TCTGCCTGGC TCAGCTGCTG 
GAGAACGAGG CTACCAACCC CTTTGAGTAC GAGTTCAGCT TCCACCTGGA GATCCGTGGA 
CCCTGTCTGC TGGCAGGAGT TGAGAGCCCC ACACATGAGC TCCGAGCAGA TGCTGACCCC 
TCTGCTCACT CTGCCAAGAG CATCATCATC ACCCTGGCCA ACAAACACAC CTTTGACAGG 
CCCGTGGAGA TCTTGATCCA CCCAAGTGAA CCCCACATGC CTCACATCTT AATGGAAGAA 
GGTGACATGA CCCCTGCAGA GTATGAGCAC CACCTGAAGG GAAAGAGTGA CTTCATTAAA 
GCCACTAAGA AGGACCCCAG CACTGAGAAA AAGACAGAAA TCATCTGGAA GCGGCTGAAC 
AAGGACATCC CCCACCACCC TGTCATCATG CTTAACTTCT GCCCTGACTT GATGACCTTC 
CAGCCAGACC TCCAGAAAGC CCAAGGAGAA TTTATCTTCC TGGTAGACCG CAGCAGGAGC 
ATGAGTGGCA TGAACATCAA CCGTGTCAAG GATGCTCTGT TGGTCATCCT TAAGAGCCTG 
ATGCCAGCGT GTCTCTTCAA CATCGTTGGG TTTGGCTCTA CCTTCAAGAC CCTCTTTCCT 
TCCAGCCAGG CTTACTGTGA GGAGAGCTTG GCCATCGCTT GCGAGAGCAT CAAGAAGATC 
CGAGCTGACA TGGGTGGCAC CAACGTCTTG TCACCATTGA GGTGGGTTGT TCGACAGCCC 
ATCCACAGGG GCCACCCCCG CTTGCTCTTT CTGCTGATGA ACGGGGCCAT CAGCAACACG 
GGGAAGGTCC TGGAGCTGCT GCAGAACCAC TCCTGCTCCA CCAGGTGCTA CAGCTTTGGC 
ATTGGGCCCA ATGCCTGCAG GAGACTGGTT GGGGGGCTGG CTGGTGTCTC CAGGGGCATC 
GCTGAGTTCC TGGTGGAAGG CGAGAGATTG CAGCCCAAGA TGATCAAATC TCTGAAGAAA 
GCAATGGCAC CAGTCCTGAG TGATGTGTCT GTGGAGTGGA TCTTCCCTGA GAGCACTGAG 
GTCTTGGTTT CTCCCATCAG CACCAGTTGC CTCTTCCCTG GTGACCACCT TGTCAGCTAC 
AGCATCATCT GTGACACCTC CCCATACATC TCCAATCCCC AGTCTGACAA GAGGCGACAT 
TACAGCATGA TGAGGTCCCA GGAGTCGGGC AGCTCTGTTT TCTTTCACTC CCAGGAGGAG 
GGAGCAGACG GGGAGAGCTG GAACCACTCC AGAGATGGGG AAAGCTACTG TCCTGCTGAG 
CATGGCCCCC ATCACCTGGT GGTTGGCAGA GAACCTGGGG GAGAATCAGA CACAGACACT 
CGAATGGAGG TGAAGGCTTT CTCCAGGAGA AGGGCTTACA GCACAACCCA AATCATTGAC 
CACAAGCCTT TCAAGAAGGT GCCCACAGCC AGCGATCCTG GCACTGACCT GGTGAAAAAT 
CCCCTCCGGA AAACCCAGCC ACAGGACCTG CAGCAGCTCA GCCCTGAGCC CTGGCAGGTG 
GATATCCAGC CCTTGCCAGC CACCTCACTT GGCTCTGCCA CCAAGATCCA TGGTGCTAGT 
GCCTGGAGGC CATCCCTGCT CCACCACAGC TGTGTGTCCT TCTGCCACCA CACCAAGTCC 
CCCGTGGCAC CTCCAAGAAG GGTCTTGGGA CCACTGGATG CTAGCACTGG CCTGAGGTCT 
TCATCGGACA GCGGAAGCCC CAGGGAGCTG GACTCTGCCC AGCATCCTCC CTTCACATTT 
GAAACAGAGA CCTCCTCTGA CTGGGAACCC CAGGACTTAG ACTTCAGTGC TTCCCATGGC 
TCCTCCTGTT CTCCCAGGAC CTTCTGCAAG GTGATGGTGA AAGGGCTGAG GAACAACGAA 
CCTGTGCAGT GGGAGGTCAC CTTTGATATC CACCCACTTT TCCAAGAGCA AGAGAGCCAG 
GCAGATGGTG ACACAGACCT GTGGAATGAG ACCTTCCACC ACCTGGCAGC CAAGTCAGTC 
ATCCGGGACT TTGAGCAGCT CGCTGAGAAG GAGTGTGACA TCGAGCATGG GTCTGGGCGC 
AGGTACCAGA TCAACACTGT CCACACCAGC AAGGCCTGCA ACATCATCAG CAAGTACACA 
ATGTTCATGC CAGTGGACCT GAGCACCAAC ACCTACCTGC CTACTGTTGT TGAGTACACC 
CACACAGGGC CATTACAGAA GCTGCCTACT GCCTCTGCAG AAGTACCAGA ATCCATTGAG 
AGCCTCTGTG CTGCCAGGCT GACCTTCAAT AAAACCAGGC TGCTGACTCA AGCTGCCAAA 
AGGTTCATGA GTAAATATCC AAGCAGAGTG GGTGAAGCCA GCTCTGAGGG CGAGAATGAG 
AAGATGGACT ACCTTCCCCT GGCATCTCTA CAGCTGGCCT GTGGTGCCTT CCTCATGAAC 
TCAGCCTTCT CTGCTGCTGT CAACATCCCC ATGGAGAAGC TGAAGTGGAC CTCTCCCTTC 
GCCTGCCACC GCTTGGCCCT CAGCCCCTCC AGCTCCTACA GCACCAAGAG GACCAACCCC 
TCCGTGGAGA AAGAAAGCCT CAGCTCCAGA CTGCAGTTCC TGGTTCCTGA TGACCACAGG 
AAGAGTCCCA CCACCAGAGA CATCCCCCTA GGAGCAGTGC CAGAGGGAGC TGGCAGCCAT 
CCTGAGGACA CTGCCCACCT TCGCCACTTC TCAGGCAGCA CGGTGGAAAG CATCTCCAAA 
GCCCTGAAAG ATGAGAAGGA ACTGTCCCCA CCAACCATTG CCATCCGCCC AGAGAGCACC 
TTGGCCTCAA CCCACCAGCA AACCAACTGT GGTGAGGGCT TGGAGATGGA TGGGTCTGAA 
GTCCAAGAGT TGCTCTTTGA CATTGAGCTG CAGCAGCAGA CAGAACCAGA GGGGATGCTC 
TGGGCCAAGG CAGTGGCCCT GGCCTGGCTG GAGCATAGCT CAGCTTCTTA TTTCATCGAG 
TGGGAGCTGG TGGCAGCCAA GGCCAACCTG TGGCTGAGCC AACAGGACTT CCCCGAGGGA 
TACAGCTTGG CCACTGTGAA AGCTGCAGCC CAGCAGCTCT TTGACCTGCT CCGGCACTGG 
GATGAGAACC TGGAGTTCAA CCTGCTGTGC TACAACCCCA ACAGCGTTTG A