polr1b cDNA ORF clone, Callorhinchus milii(elephant shark)

Gene
Clones
gRNAs

Summary

Gene Symbol	polr1b
Entrez Gene ID	103178931
Full Name	RNA polymerase I subunit B
Gene Type	protein-coding
Organism	Callorhinchus milii(elephant shark)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007893921.1	XP_007892112.1	DNA-directed RNA polymerase I subunit RPA2 isoform X1
XM_007893922.1	XP_007892113.1	DNA-directed RNA polymerase I subunit RPA2 isoform X2

Pathways from BioSystems
Gene Ontology

The following polr1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the polr1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa376515	XM_007893921.1 Latest version!	Callorhinchus milii polymerase (RNA) I polypeptide B, 128kDa (polr1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCa376516	XM_007893922.1 Latest version!	Callorhinchus milii polymerase (RNA) I polypeptide B, 128kDa (polr1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

polr1b ( XM_007893921.1 ) cDNA ORF clone, Callorhinchus milii(elephant shark) -> XP_007892112.1 Callorhinchus milii polymerase (RNA) I polypeptide B, 128kDa (polr1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCa376515
Clone ID Related Accession (Same CDS sequence)	XM_007893921.1
Accession Version	XM_007893921.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-05-08
Organism	Callorhinchus milii(elephant shark)
Product	DNA-directed RNA polymerase I subunit RPA2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006890084.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Callorhinchus milii Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACG GCAAGTGGCG CAGTGCGATT CCCCCACTGG AGTTCAACTT CAAAAATGAA 
CGTATCTCTC TGGCGTTTGT GGATGCTGTC ATCTCCCCTC CTATGGTGCC TAAAGGCTGC 
ATCTGCAGTG ACCTGAAAAT CTACCCAGCA GAGTGCAGAG GACGGCGGAG TACTTACAAG 
GGGAAGCTAA CTGCAGACGT TAGTTGGTCA GTGAATGGCT TACCCAAAGG GATTATTAAA 
CAGAGCCTTG GTATGATCCC TATCATGGTT AAATCCAAAC TATGTAACCT GCACGGCTTG 
TCTCCTCGAG GTCTCATTAA ACACCACGAA GAAGCAGAGG AAATGGGGGG ATACTTCATA 
ATAAATGGCA TAGAAAAGAT CATCAGAATG CTCATCATGC CAAGACGCAA TTTCCCAATT 
GCTATGGTGA GGCCCAAGTG GAAGAATAGA GGCCCTGGCT ACACCCAGTA CGGTGTCAGC 
ATGCGCTGTG TGAGAGATGA ACACACTGCC ATTAACATGA ACCTTCACTA TCTGGACAAT 
GGCAGTGTAA TGGTCAACTT CATCTACCAG AAAGAACTCT TCTTCCTCCC TCTATGCTTT 
GCACTCAAGG GGTTGGTTGA TTTCACGGAT TACCAGATTT ACCAGGAGTT AATTAAAGGA 
AGAGAGGACA ACTCCTTCTT CAAGAGCGCT GTGACGCAGA TGTTGAGACT GGTAGTGATG 
GAGGGTTGCA ACACCAAGAA GCAAGCATTA AATTACCTGG GAGAGAGGTT CAGTGTGAAG 
CTCCGCCTGC CAGACTGGTA CACAAATGAG CAAGCTGCAG AGTTCCTGTT TGACCAGTGC 
TTGTGCATAC ACCTGAAGGC CAACGTGGAG AAATTTTATT TGCTTTGTCT GATGACGCGG 
AAGCTGTTTA CGTTCGCCAA GGATGAATGT CAGGAAGAAA ATCCCGACAG CTTGATAAAT 
CATGAAGTGC TGACGCCTGG GCAGCTCATT TTAATGTTTT TAAAGGAGAA AATGACTTGG 
TGGTTACAAT CTATAAAATT TGCAATGGAC AAGAAGAATC AAAAAGGAGC CTTTCCTATG 
AATTCAGAAA ACTTGATGAA AGCCTTAAGT ATGGTGGGAG ATCTAACCCG GCCATTTGAG 
TATCTTTTGG CTACTGGAAA CTTGCACTCA AAAACAGGTC TGGGTATGTT GCAGGATTCT 
GGTTTGTGTG TTGTGGCAGA CAAGCTGAAT TTCGTCCGTT ACCTCTCTCA TTTCCGATGC 
GTGCATAGAG GGGCTGCTTT TGCTAAGATG AGAACCACCT CTGTGCGTAA GTTACTCCCC 
GAGTCCTGGG GCTTCCTGTG CCCTGTGCAC ACTCCAGATG GAGAGCCTTG TGGGCTGATG 
AACCATTTGA CTGCCATTTG CGAGATTGTG ACCCAGTCAT GGAGCACCGT CTCTCTGCCA 
GCTCTGCTTT GTGCCCTGGG TGTTACACCA GTGGATGGCA CGCCCAACAG TCCGTATTCT 
GAGTGCTACT CAGTTCTTCT GGACGGCTCC GTTGTTGGGT GGGTGGAGAG AGAGATGGGA 
CCACACGTCG CGGATACTCT GCGCACCTTC AAGGTTTTAA AAGAGAAGAA AGTCCCTCTA 
TGGATTGAAA TTGTTCTGAT TCCAATGACG GGTAAACCCA GTATTTACCC AGGATTGTAT 
CTCTTCACCA CTCCTTGTCG GATGATACGA CCTGTCAGAA ACTTGGCCTT GGGAAAGGAA 
GAGCTCATTG GCACCCTTGA ACAGCTGTAC ATGAATATTG CCATCACAGA ACAGGATATT 
GTGCCTGGAG GAACCACTCA TCAGGAGCTA TTTCCTCACA GTATGCTGAG CGTAGTGGCG 
AACTTAATTC CTTACTCTGA TCATAACCAG AGTCCTAGGA ACATGTACCA GTGTCAGATG 
GGAAAGCAAA CAATGGGATT CCCATTGCAC AGCTACCTGT ATCGATCCGA CAACAAACTC 
TACCGTCTTC TCACTCCCCA GAGCCCTCTT GTCCGACCTT ACATGTATGA CTACTTTAAC 
ATGGATAACT ATCCCATAGG CACTAATGCT GTTGTAGCTG TCATCTCGTA CACAGGATAT 
GACATGGAAG ATGCTATGAT CGTCAACAAA GCATCTTGGG AGAGAGGATT TGCCTATGGA 
AGCGTTTATA AGACTGAATT GATCAATCTC GATCAAAAAG TGACAGCTGG AGACTCTAAT 
TTGGTCTTCG GTGTCAAACC CGGCGACACA CGAGTGGGAC AGAAACTCGA CGAGGACGGA 
TTGCCAGCGA TAGGAGCCGT GCTGGAGTAC GGTGATCCCT TCTATAGTTA CTTGAATCTC 
AACACAGGGG ATAGCTTTGT GATGTACTAC AAGAGCAAAG AAAGTTGTGT TGTGGATAAC 
ATCAAAGTTT GCAGCAATGA TAATGGGACA GCAACTTTTA AACGTGTGTG CATCACCTTG 
AGAATCCCCC GTAACCCCAC CATTGGAGAC AAGTTTGCTA GTCGCCACGG GCAAAAGGGC 
ATCTTGAGCC GACTGTGGCC AGCAGAGGAC ATGCCGTTCA CAGAGAGTGG TATGATGCCA 
GATATCCTGT TTAATCCCCA CGGATTCCCT TCCCGTATGA CCATAGGAAT GCTGATAGAA 
AGCATGGCAG GTAAATCTGC TGCCATGCAT GGCCTGTGTC ACGATGCCAC ACCATTCACG 
TTCTCTGAGG AAAATTCAGC CTTGGAGTAC TTTGGGAAGA TGCTGCAGGC TTCCGGCTAT 
AACTTCTATG GGACTGAAAG AATGTACAGT GGGATCAGTG GTGTGGAGCT GGAGGCAGAC 
ATCTTCATTG GAGTGGTTTA CTACCAGCGC CTACGTCACA TGGTCTCTGA CAAGTTCCAG 
GTTAGAACTA CTGGAGCGAG AGATAGGCTG ACAAACCAGC CCGTTGGAGG GAGAAACGTC 
CAAGGTGGCA TCCGATTTGG AGAGATGGAG CGCGATGCTC TACTAGCTCA TGGAACGTCC 
TTCTTACTTC AAGACCGCCT CTTCAATTGC TCGGATCGCT CCGTAGCACA CGTCTGTATT 
AAGTGTGGCA GCCTCCTGTC GCCAATACTG GAAAAGCCCC CACCATCGTG GTCTGCCACA 
CGTCACAGGA AACATCACTG CACTCTCTGT AAACAGTCTG ACACTGTTGA GATTATTTCT 
GTGCCTTATG TTTTCCGATA TTTTGTGGCG GAACTTGCAG CGATGAACAT TAAAATTCGA 
CTGGATGTGA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007892112.1
CDS	30..3284
Translation	MEDGKWRSAIPPLEFNFKNERISLAFVDAVISPPMVPKGCICSDLKIYPAECRGRRSTYKGKLTADVSWSVNGLPKGIIKQSLGMIPIMVKSKLCNLHGLSPRGLIKHHEEAEEMGGYFIINGIEKIIRMLIMPRRNFPIAMVRPKWKNRGPGYTQYGVSMRCVRDEHTAINMNLHYLDNGSVMVNFIYQKELFFLPLCFALKGLVDFTDYQIYQELIKGREDNSFFKSAVTQMLRLVVMEGCNTKKQALNYLGERFSVKLRLPDWYTNEQAAEFLFDQCLCIHLKANVEKFYLLCLMTRKLFTFAKDECQEENPDSLINHEVLTPGQLILMFLKEKMTWWLQSIKFAMDKKNQKGAFPMNSENLMKALSMVGDLTRPFEYLLATGNLHSKTGLGMLQDSGLCVVADKLNFVRYLSHFRCVHRGAAFAKMRTTSVRKLLPESWGFLCPVHTPDGEPCGLMNHLTAICEIVTQSWSTVSLPALLCALGVTPVDGTPNSPYSECYSVLLDGSVVGWVEREMGPHVADTLRTFKVLKEKKVPLWIEIVLIPMTGKPSIYPGLYLFTTPCRMIRPVRNLALGKEELIGTLEQLYMNIAITEQDIVPGGTTHQELFPHSMLSVVANLIPYSDHNQSPRNMYQCQMGKQTMGFPLHSYLYRSDNKLYRLLTPQSPLVRPYMYDYFNMDNYPIGTNAVVAVISYTGYDMEDAMIVNKASWERGFAYGSVYKTELINLDQKVTAGDSNLVFGVKPGDTRVGQKLDEDGLPAIGAVLEYGDPFYSYLNLNTGDSFVMYYKSKESCVVDNIKVCSNDNGTATFKRVCITLRIPRNPTIGDKFASRHGQKGILSRLWPAEDMPFTESGMMPDILFNPHGFPSRMTIGMLIESMAGKSAAMHGLCHDATPFTFSEENSALEYFGKMLQASGYNFYGTERMYSGISGVELEADIFIGVVYYQRLRHMVSDKFQVRTTGARDRLTNQPVGGRNVQGGIRFGEMERDALLAHGTSFLLQDRLFNCSDRSVAHVCIKCGSLLSPILEKPPPSWSATRHRKHHCTLCKQSDTVEIISVPYVFRYFVAELAAMNIKIRLDVS

Target ORF information:

RefSeq Version	XM_007893921.1
Organism	Callorhinchus milii(elephant shark)
Definition	Callorhinchus milii polymerase (RNA) I polypeptide B, 128kDa (polr1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007893921.1

ORF Insert Sequence:

ATGGAGGACG GCAAGTGGCG CAGTGCGATT CCCCCACTGG AGTTCAACTT CAAAAATGAA 
CGTATCTCTC TGGCGTTTGT GGATGCTGTC ATCTCCCCTC CTATGGTGCC TAAAGGCTGC 
ATCTGCAGTG ACCTGAAAAT CTACCCAGCA GAGTGCAGAG GACGGCGGAG TACTTACAAG 
GGGAAGCTAA CTGCAGACGT TAGTTGGTCA GTGAATGGCT TACCCAAAGG GATTATTAAA 
CAGAGCCTTG GTATGATCCC TATCATGGTT AAATCCAAAC TATGTAACCT GCACGGCTTG 
TCTCCTCGAG GTCTCATTAA ACACCACGAA GAAGCAGAGG AAATGGGGGG ATACTTCATA 
ATAAATGGCA TAGAAAAGAT CATCAGAATG CTCATCATGC CAAGACGCAA TTTCCCAATT 
GCTATGGTGA GGCCCAAGTG GAAGAATAGA GGCCCTGGCT ACACCCAGTA CGGTGTCAGC 
ATGCGCTGTG TGAGAGATGA ACACACTGCC ATTAACATGA ACCTTCACTA TCTGGACAAT 
GGCAGTGTAA TGGTCAACTT CATCTACCAG AAAGAACTCT TCTTCCTCCC TCTATGCTTT 
GCACTCAAGG GGTTGGTTGA TTTCACGGAT TACCAGATTT ACCAGGAGTT AATTAAAGGA 
AGAGAGGACA ACTCCTTCTT CAAGAGCGCT GTGACGCAGA TGTTGAGACT GGTAGTGATG 
GAGGGTTGCA ACACCAAGAA GCAAGCATTA AATTACCTGG GAGAGAGGTT CAGTGTGAAG 
CTCCGCCTGC CAGACTGGTA CACAAATGAG CAAGCTGCAG AGTTCCTGTT TGACCAGTGC 
TTGTGCATAC ACCTGAAGGC CAACGTGGAG AAATTTTATT TGCTTTGTCT GATGACGCGG 
AAGCTGTTTA CGTTCGCCAA GGATGAATGT CAGGAAGAAA ATCCCGACAG CTTGATAAAT 
CATGAAGTGC TGACGCCTGG GCAGCTCATT TTAATGTTTT TAAAGGAGAA AATGACTTGG 
TGGTTACAAT CTATAAAATT TGCAATGGAC AAGAAGAATC AAAAAGGAGC CTTTCCTATG 
AATTCAGAAA ACTTGATGAA AGCCTTAAGT ATGGTGGGAG ATCTAACCCG GCCATTTGAG 
TATCTTTTGG CTACTGGAAA CTTGCACTCA AAAACAGGTC TGGGTATGTT GCAGGATTCT 
GGTTTGTGTG TTGTGGCAGA CAAGCTGAAT TTCGTCCGTT ACCTCTCTCA TTTCCGATGC 
GTGCATAGAG GGGCTGCTTT TGCTAAGATG AGAACCACCT CTGTGCGTAA GTTACTCCCC 
GAGTCCTGGG GCTTCCTGTG CCCTGTGCAC ACTCCAGATG GAGAGCCTTG TGGGCTGATG 
AACCATTTGA CTGCCATTTG CGAGATTGTG ACCCAGTCAT GGAGCACCGT CTCTCTGCCA 
GCTCTGCTTT GTGCCCTGGG TGTTACACCA GTGGATGGCA CGCCCAACAG TCCGTATTCT 
GAGTGCTACT CAGTTCTTCT GGACGGCTCC GTTGTTGGGT GGGTGGAGAG AGAGATGGGA 
CCACACGTCG CGGATACTCT GCGCACCTTC AAGGTTTTAA AAGAGAAGAA AGTCCCTCTA 
TGGATTGAAA TTGTTCTGAT TCCAATGACG GGTAAACCCA GTATTTACCC AGGATTGTAT 
CTCTTCACCA CTCCTTGTCG GATGATACGA CCTGTCAGAA ACTTGGCCTT GGGAAAGGAA 
GAGCTCATTG GCACCCTTGA ACAGCTGTAC ATGAATATTG CCATCACAGA ACAGGATATT 
GTGCCTGGAG GAACCACTCA TCAGGAGCTA TTTCCTCACA GTATGCTGAG CGTAGTGGCG 
AACTTAATTC CTTACTCTGA TCATAACCAG AGTCCTAGGA ACATGTACCA GTGTCAGATG 
GGAAAGCAAA CAATGGGATT CCCATTGCAC AGCTACCTGT ATCGATCCGA CAACAAACTC 
TACCGTCTTC TCACTCCCCA GAGCCCTCTT GTCCGACCTT ACATGTATGA CTACTTTAAC 
ATGGATAACT ATCCCATAGG CACTAATGCT GTTGTAGCTG TCATCTCGTA CACAGGATAT 
GACATGGAAG ATGCTATGAT CGTCAACAAA GCATCTTGGG AGAGAGGATT TGCCTATGGA 
AGCGTTTATA AGACTGAATT GATCAATCTC GATCAAAAAG TGACAGCTGG AGACTCTAAT 
TTGGTCTTCG GTGTCAAACC CGGCGACACA CGAGTGGGAC AGAAACTCGA CGAGGACGGA 
TTGCCAGCGA TAGGAGCCGT GCTGGAGTAC GGTGATCCCT TCTATAGTTA CTTGAATCTC 
AACACAGGGG ATAGCTTTGT GATGTACTAC AAGAGCAAAG AAAGTTGTGT TGTGGATAAC 
ATCAAAGTTT GCAGCAATGA TAATGGGACA GCAACTTTTA AACGTGTGTG CATCACCTTG 
AGAATCCCCC GTAACCCCAC CATTGGAGAC AAGTTTGCTA GTCGCCACGG GCAAAAGGGC 
ATCTTGAGCC GACTGTGGCC AGCAGAGGAC ATGCCGTTCA CAGAGAGTGG TATGATGCCA 
GATATCCTGT TTAATCCCCA CGGATTCCCT TCCCGTATGA CCATAGGAAT GCTGATAGAA 
AGCATGGCAG GTAAATCTGC TGCCATGCAT GGCCTGTGTC ACGATGCCAC ACCATTCACG 
TTCTCTGAGG AAAATTCAGC CTTGGAGTAC TTTGGGAAGA TGCTGCAGGC TTCCGGCTAT 
AACTTCTATG GGACTGAAAG AATGTACAGT GGGATCAGTG GTGTGGAGCT GGAGGCAGAC 
ATCTTCATTG GAGTGGTTTA CTACCAGCGC CTACGTCACA TGGTCTCTGA CAAGTTCCAG 
GTTAGAACTA CTGGAGCGAG AGATAGGCTG ACAAACCAGC CCGTTGGAGG GAGAAACGTC 
CAAGGTGGCA TCCGATTTGG AGAGATGGAG CGCGATGCTC TACTAGCTCA TGGAACGTCC 
TTCTTACTTC AAGACCGCCT CTTCAATTGC TCGGATCGCT CCGTAGCACA CGTCTGTATT 
AAGTGTGGCA GCCTCCTGTC GCCAATACTG GAAAAGCCCC CACCATCGTG GTCTGCCACA 
CGTCACAGGA AACATCACTG CACTCTCTGT AAACAGTCTG ACACTGTTGA GATTATTTCT 
GTGCCTTATG TTTTCCGATA TTTTGTGGCG GAACTTGCAG CGATGAACAT TAAAATTCGA 
CTGGATGTGA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

polr1b ( XM_007893922.1 ) cDNA ORF clone, Callorhinchus milii(elephant shark) -> XP_007892113.1 Callorhinchus milii polymerase (RNA) I polypeptide B, 128kDa (polr1b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCa376516
Clone ID Related Accession (Same CDS sequence)	XM_007893922.1
Accession Version	XM_007893922.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-05-08
Organism	Callorhinchus milii(elephant shark)
Product	DNA-directed RNA polymerase I subunit RPA2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006890084.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Callorhinchus milii Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACG GCAAGTGGCG CAGTGTGAGT GAGGAGCAGC GGTGGCCGAG CCTGCGGGGC 
CTGACCCAGG CCGGGGGGGG CCGCCCGGGG CCGCGGCAGC ACCAGGCGCT GCAGGAGCTG 
GTCAGGGCCC ACGTGGACTC CTTCGACCAG GCGACCGGCG ACGGTTTCAA TCCCTTCTTG 
CAGGCGATTC CCCCACTGGA GTTCAACTTC AAAAATGAAC GTATCTCTCT GGCGTTTGTG 
GATGCTGTCA TCTCCCCTCC TATGGTGCCT AAAGGCTGCA TCTGCAGTGA CCTGAAAATC 
TACCCAGCAG AGTGCAGAGG ACGGCGGAGT ACTTACAAGG GGAAGCTAAC TGCAGACGTT 
AGTTGGTCAG TGAATGGCTT ACCCAAAGGG ATTATTAAAC AGAGCCTTGG TATGATCCCT 
ATCATGGTTA AATCCAAACT ATGTAACCTG CACGGCTTGT CTCCTCGAGG TCTCATTAAA 
CACCACGAAG AAGCAGAGGA AATGGGGGGA TACTTCATAA TAAATGGCAT AGAAAAGATC 
ATCAGAATGC TCATCATGCC AAGACGCAAT TTCCCAATTG CTATGGTGAG GCCCAAGTGG 
AAGAATAGAG GCCCTGGCTA CACCCAGTAC GGTGTCAGCA TGCGCTGTGT GAGAGATGAA 
CACACTGCCA TTAACATGAA CCTTCACTAT CTGGACAATG GCAGTGTAAT GGTCAACTTC 
ATCTACCAGA AAGAACTCTT CTTCCTCCCT CTATGCTTTG CACTCAAGGG GTTGGTTGAT 
TTCACGGATT ACCAGATTTA CCAGGAGTTA ATTAAAGGAA GAGAGGACAA CTCCTTCTTC 
AAGAGCGCTG TGACGCAGAT GTTGAGACTG GTAGTGATGG AGGGTTGCAA CACCAAGAAG 
CAAGCATTAA ATTACCTGGG AGAGAGGTTC AGTGTGAAGC TCCGCCTGCC AGACTGGTAC 
ACAAATGAGC AAGCTGCAGA GTTCCTGTTT GACCAGTGCT TGTGCATACA CCTGAAGGCC 
AACGTGGAGA AATTTTATTT GCTTTGTCTG ATGACGCGGA AGCTGTTTAC GTTCGCCAAG 
GATGAATGTC AGGAAGAAAA TCCCGACAGC TTGATAAATC ATGAAGTGCT GACGCCTGGG 
CAGCTCATTT TAATGTTTTT AAAGGAGAAA ATGACTTGGT GGTTACAATC TATAAAATTT 
GCAATGGACA AGAAGAATCA AAAAGGAGCC TTTCCTATGA ATTCAGAAAA CTTGATGAAA 
GCCTTAAGTA TGGTGGGAGA TCTAACCCGG CCATTTGAGT ATCTTTTGGC TACTGGAAAC 
TTGCACTCAA AAACAGGTCT GGGTATGTTG CAGGATTCTG GTTTGTGTGT TGTGGCAGAC 
AAGCTGAATT TCGTCCGTTA CCTCTCTCAT TTCCGATGCG TGCATAGAGG GGCTGCTTTT 
GCTAAGATGA GAACCACCTC TGTGCGTAAG TTACTCCCCG AGTCCTGGGG CTTCCTGTGC 
CCTGTGCACA CTCCAGATGG AGAGCCTTGT GGGCTGATGA ACCATTTGAC TGCCATTTGC 
GAGATTGTGA CCCAGTCATG GAGCACCGTC TCTCTGCCAG CTCTGCTTTG TGCCCTGGGT 
GTTACACCAG TGGATGGCAC GCCCAACAGT CCGTATTCTG AGTGCTACTC AGTTCTTCTG 
GACGGCTCCG TTGTTGGGTG GGTGGAGAGA GAGATGGGAC CACACGTCGC GGATACTCTG 
CGCACCTTCA AGGTTTTAAA AGAGAAGAAA GTCCCTCTAT GGATTGAAAT TGTTCTGATT 
CCAATGACGG GTAAACCCAG TATTTACCCA GGATTGTATC TCTTCACCAC TCCTTGTCGG 
ATGATACGAC CTGTCAGAAA CTTGGCCTTG GGAAAGGAAG AGCTCATTGG CACCCTTGAA 
CAGCTGTACA TGAATATTGC CATCACAGAA CAGGATATTG TGCCTGGAGG AACCACTCAT 
CAGGAGCTAT TTCCTCACAG TATGCTGAGC GTAGTGGCGA ACTTAATTCC TTACTCTGAT 
CATAACCAGA GTCCTAGGAA CATGTACCAG TGTCAGATGG GAAAGCAAAC AATGGGATTC 
CCATTGCACA GCTACCTGTA TCGATCCGAC AACAAACTCT ACCGTCTTCT CACTCCCCAG 
AGCCCTCTTG TCCGACCTTA CATGTATGAC TACTTTAACA TGGATAACTA TCCCATAGGC 
ACTAATGCTG TTGTAGCTGT CATCTCGTAC ACAGGATATG ACATGGAAGA TGCTATGATC 
GTCAACAAAG CATCTTGGGA GAGAGGATTT GCCTATGGAA GCGTTTATAA GACTGAATTG 
ATCAATCTCG ATCAAAAAGT GACAGCTGGA GACTCTAATT TGGTCTTCGG TGTCAAACCC 
GGCGACACAC GAGTGGGACA GAAACTCGAC GAGGACGGAT TGCCAGCGAT AGGAGCCGTG 
CTGGAGTACG GTGATCCCTT CTATAGTTAC TTGAATCTCA ACACAGGGGA TAGCTTTGTG 
ATGTACTACA AGAGCAAAGA AAGTTGTGTT GTGGATAACA TCAAAGTTTG CAGCAATGAT 
AATGGGACAG CAACTTTTAA ACGTGTGTGC ATCACCTTGA GAATCCCCCG TAACCCCACC 
ATTGGAGACA AGTTTGCTAG TCGCCACGGG CAAAAGGGCA TCTTGAGCCG ACTGTGGCCA 
GCAGAGGACA TGCCGTTCAC AGAGAGTGGT ATGATGCCAG ATATCCTGTT TAATCCCCAC 
GGATTCCCTT CCCGTATGAC CATAGGAATG CTGATAGAAA GCATGGCAGG TAAATCTGCT 
GCCATGCATG GCCTGTGTCA CGATGCCACA CCATTCACGT TCTCTGAGGA AAATTCAGCC 
TTGGAGTACT TTGGGAAGAT GCTGCAGGCT TCCGGCTATA ACTTCTATGG GACTGAAAGA 
ATGTACAGTG GGATCAGTGG TGTGGAGCTG GAGGCAGACA TCTTCATTGG AGTGGTTTAC 
TACCAGCGCC TACGTCACAT GGTCTCTGAC AAGTTCCAGG TTAGAACTAC TGGAGCGAGA 
GATAGGCTGA CAAACCAGCC CGTTGGAGGG AGAAACGTCC AAGGTGGCAT CCGATTTGGA 
GAGATGGAGC GCGATGCTCT ACTAGCTCAT GGAACGTCCT TCTTACTTCA AGACCGCCTC 
TTCAATTGCT CGGATCGCTC CGTAGCACAC GTCTGTATTA AGTGTGGCAG CCTCCTGTCG 
CCAATACTGG AAAAGCCCCC ACCATCGTGG TCTGCCACAC GTCACAGGAA ACATCACTGC 
ACTCTCTGTA AACAGTCTGA CACTGTTGAG ATTATTTCTG TGCCTTATGT TTTCCGATAT 
TTTGTGGCGG AACTTGCAGC GATGAACATT AAAATTCGAC TGGATGTGAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007892113.1
CDS	41..3454
Translation	MEDGKWRSVSEEQRWPSLRGLTQAGGGRPGPRQHQALQELVRAHVDSFDQATGDGFNPFLQAIPPLEFNFKNERISLAFVDAVISPPMVPKGCICSDLKIYPAECRGRRSTYKGKLTADVSWSVNGLPKGIIKQSLGMIPIMVKSKLCNLHGLSPRGLIKHHEEAEEMGGYFIINGIEKIIRMLIMPRRNFPIAMVRPKWKNRGPGYTQYGVSMRCVRDEHTAINMNLHYLDNGSVMVNFIYQKELFFLPLCFALKGLVDFTDYQIYQELIKGREDNSFFKSAVTQMLRLVVMEGCNTKKQALNYLGERFSVKLRLPDWYTNEQAAEFLFDQCLCIHLKANVEKFYLLCLMTRKLFTFAKDECQEENPDSLINHEVLTPGQLILMFLKEKMTWWLQSIKFAMDKKNQKGAFPMNSENLMKALSMVGDLTRPFEYLLATGNLHSKTGLGMLQDSGLCVVADKLNFVRYLSHFRCVHRGAAFAKMRTTSVRKLLPESWGFLCPVHTPDGEPCGLMNHLTAICEIVTQSWSTVSLPALLCALGVTPVDGTPNSPYSECYSVLLDGSVVGWVEREMGPHVADTLRTFKVLKEKKVPLWIEIVLIPMTGKPSIYPGLYLFTTPCRMIRPVRNLALGKEELIGTLEQLYMNIAITEQDIVPGGTTHQELFPHSMLSVVANLIPYSDHNQSPRNMYQCQMGKQTMGFPLHSYLYRSDNKLYRLLTPQSPLVRPYMYDYFNMDNYPIGTNAVVAVISYTGYDMEDAMIVNKASWERGFAYGSVYKTELINLDQKVTAGDSNLVFGVKPGDTRVGQKLDEDGLPAIGAVLEYGDPFYSYLNLNTGDSFVMYYKSKESCVVDNIKVCSNDNGTATFKRVCITLRIPRNPTIGDKFASRHGQKGILSRLWPAEDMPFTESGMMPDILFNPHGFPSRMTIGMLIESMAGKSAAMHGLCHDATPFTFSEENSALEYFGKMLQASGYNFYGTERMYSGISGVELEADIFIGVVYYQRLRHMVSDKFQVRTTGARDRLTNQPVGGRNVQGGIRFGEMERDALLAHGTSFLLQDRLFNCSDRSVAHVCIKCGSLLSPILEKPPPSWSATRHRKHHCTLCKQSDTVEIISVPYVFRYFVAELAAMNIKIRLDVS

Target ORF information:

RefSeq Version	XM_007893922.1
Organism	Callorhinchus milii(elephant shark)
Definition	Callorhinchus milii polymerase (RNA) I polypeptide B, 128kDa (polr1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007893922.1

ORF Insert Sequence:

ATGGAGGACG GCAAGTGGCG CAGTGTGAGT GAGGAGCAGC GGTGGCCGAG CCTGCGGGGC 
CTGACCCAGG CCGGGGGGGG CCGCCCGGGG CCGCGGCAGC ACCAGGCGCT GCAGGAGCTG 
GTCAGGGCCC ACGTGGACTC CTTCGACCAG GCGACCGGCG ACGGTTTCAA TCCCTTCTTG 
CAGGCGATTC CCCCACTGGA GTTCAACTTC AAAAATGAAC GTATCTCTCT GGCGTTTGTG 
GATGCTGTCA TCTCCCCTCC TATGGTGCCT AAAGGCTGCA TCTGCAGTGA CCTGAAAATC 
TACCCAGCAG AGTGCAGAGG ACGGCGGAGT ACTTACAAGG GGAAGCTAAC TGCAGACGTT 
AGTTGGTCAG TGAATGGCTT ACCCAAAGGG ATTATTAAAC AGAGCCTTGG TATGATCCCT 
ATCATGGTTA AATCCAAACT ATGTAACCTG CACGGCTTGT CTCCTCGAGG TCTCATTAAA 
CACCACGAAG AAGCAGAGGA AATGGGGGGA TACTTCATAA TAAATGGCAT AGAAAAGATC 
ATCAGAATGC TCATCATGCC AAGACGCAAT TTCCCAATTG CTATGGTGAG GCCCAAGTGG 
AAGAATAGAG GCCCTGGCTA CACCCAGTAC GGTGTCAGCA TGCGCTGTGT GAGAGATGAA 
CACACTGCCA TTAACATGAA CCTTCACTAT CTGGACAATG GCAGTGTAAT GGTCAACTTC 
ATCTACCAGA AAGAACTCTT CTTCCTCCCT CTATGCTTTG CACTCAAGGG GTTGGTTGAT 
TTCACGGATT ACCAGATTTA CCAGGAGTTA ATTAAAGGAA GAGAGGACAA CTCCTTCTTC 
AAGAGCGCTG TGACGCAGAT GTTGAGACTG GTAGTGATGG AGGGTTGCAA CACCAAGAAG 
CAAGCATTAA ATTACCTGGG AGAGAGGTTC AGTGTGAAGC TCCGCCTGCC AGACTGGTAC 
ACAAATGAGC AAGCTGCAGA GTTCCTGTTT GACCAGTGCT TGTGCATACA CCTGAAGGCC 
AACGTGGAGA AATTTTATTT GCTTTGTCTG ATGACGCGGA AGCTGTTTAC GTTCGCCAAG 
GATGAATGTC AGGAAGAAAA TCCCGACAGC TTGATAAATC ATGAAGTGCT GACGCCTGGG 
CAGCTCATTT TAATGTTTTT AAAGGAGAAA ATGACTTGGT GGTTACAATC TATAAAATTT 
GCAATGGACA AGAAGAATCA AAAAGGAGCC TTTCCTATGA ATTCAGAAAA CTTGATGAAA 
GCCTTAAGTA TGGTGGGAGA TCTAACCCGG CCATTTGAGT ATCTTTTGGC TACTGGAAAC 
TTGCACTCAA AAACAGGTCT GGGTATGTTG CAGGATTCTG GTTTGTGTGT TGTGGCAGAC 
AAGCTGAATT TCGTCCGTTA CCTCTCTCAT TTCCGATGCG TGCATAGAGG GGCTGCTTTT 
GCTAAGATGA GAACCACCTC TGTGCGTAAG TTACTCCCCG AGTCCTGGGG CTTCCTGTGC 
CCTGTGCACA CTCCAGATGG AGAGCCTTGT GGGCTGATGA ACCATTTGAC TGCCATTTGC 
GAGATTGTGA CCCAGTCATG GAGCACCGTC TCTCTGCCAG CTCTGCTTTG TGCCCTGGGT 
GTTACACCAG TGGATGGCAC GCCCAACAGT CCGTATTCTG AGTGCTACTC AGTTCTTCTG 
GACGGCTCCG TTGTTGGGTG GGTGGAGAGA GAGATGGGAC CACACGTCGC GGATACTCTG 
CGCACCTTCA AGGTTTTAAA AGAGAAGAAA GTCCCTCTAT GGATTGAAAT TGTTCTGATT 
CCAATGACGG GTAAACCCAG TATTTACCCA GGATTGTATC TCTTCACCAC TCCTTGTCGG 
ATGATACGAC CTGTCAGAAA CTTGGCCTTG GGAAAGGAAG AGCTCATTGG CACCCTTGAA 
CAGCTGTACA TGAATATTGC CATCACAGAA CAGGATATTG TGCCTGGAGG AACCACTCAT 
CAGGAGCTAT TTCCTCACAG TATGCTGAGC GTAGTGGCGA ACTTAATTCC TTACTCTGAT 
CATAACCAGA GTCCTAGGAA CATGTACCAG TGTCAGATGG GAAAGCAAAC AATGGGATTC 
CCATTGCACA GCTACCTGTA TCGATCCGAC AACAAACTCT ACCGTCTTCT CACTCCCCAG 
AGCCCTCTTG TCCGACCTTA CATGTATGAC TACTTTAACA TGGATAACTA TCCCATAGGC 
ACTAATGCTG TTGTAGCTGT CATCTCGTAC ACAGGATATG ACATGGAAGA TGCTATGATC 
GTCAACAAAG CATCTTGGGA GAGAGGATTT GCCTATGGAA GCGTTTATAA GACTGAATTG 
ATCAATCTCG ATCAAAAAGT GACAGCTGGA GACTCTAATT TGGTCTTCGG TGTCAAACCC 
GGCGACACAC GAGTGGGACA GAAACTCGAC GAGGACGGAT TGCCAGCGAT AGGAGCCGTG 
CTGGAGTACG GTGATCCCTT CTATAGTTAC TTGAATCTCA ACACAGGGGA TAGCTTTGTG 
ATGTACTACA AGAGCAAAGA AAGTTGTGTT GTGGATAACA TCAAAGTTTG CAGCAATGAT 
AATGGGACAG CAACTTTTAA ACGTGTGTGC ATCACCTTGA GAATCCCCCG TAACCCCACC 
ATTGGAGACA AGTTTGCTAG TCGCCACGGG CAAAAGGGCA TCTTGAGCCG ACTGTGGCCA 
GCAGAGGACA TGCCGTTCAC AGAGAGTGGT ATGATGCCAG ATATCCTGTT TAATCCCCAC 
GGATTCCCTT CCCGTATGAC CATAGGAATG CTGATAGAAA GCATGGCAGG TAAATCTGCT 
GCCATGCATG GCCTGTGTCA CGATGCCACA CCATTCACGT TCTCTGAGGA AAATTCAGCC 
TTGGAGTACT TTGGGAAGAT GCTGCAGGCT TCCGGCTATA ACTTCTATGG GACTGAAAGA 
ATGTACAGTG GGATCAGTGG TGTGGAGCTG GAGGCAGACA TCTTCATTGG AGTGGTTTAC 
TACCAGCGCC TACGTCACAT GGTCTCTGAC AAGTTCCAGG TTAGAACTAC TGGAGCGAGA 
GATAGGCTGA CAAACCAGCC CGTTGGAGGG AGAAACGTCC AAGGTGGCAT CCGATTTGGA 
GAGATGGAGC GCGATGCTCT ACTAGCTCAT GGAACGTCCT TCTTACTTCA AGACCGCCTC 
TTCAATTGCT CGGATCGCTC CGTAGCACAC GTCTGTATTA AGTGTGGCAG CCTCCTGTCG 
CCAATACTGG AAAAGCCCCC ACCATCGTGG TCTGCCACAC GTCACAGGAA ACATCACTGC 
ACTCTCTGTA AACAGTCTGA CACTGTTGAG ATTATTTCTG TGCCTTATGT TTTCCGATAT 
TTTGTGGCGG AACTTGCAGC GATGAACATT AAAATTCGAC TGGATGTGAG CTAA