GNAI2 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	GNAI2
Entrez Gene ID	106898288
Full Name	G protein subunit alpha i2
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014959445.1	XP_014814931.1	guanine nucleotide-binding protein G(i) subunit alpha-2

Pathways from BioSystems
Gene Ontology

The following GNAI2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GNAI2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa77824	XM_014959445.1 Latest version!	Calidris pugnax guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 (GNAI2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GNAI2 ( XM_014959445.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014814931.1 Calidris pugnax guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 (GNAI2), mRNA.

Price & Availability↑

CloneID	OCa77824
Clone ID Related Accession (Same CDS sequence)	XM_014959445.1
Accession Version	XM_014959445.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	guanine nucleotide-binding protein G(i) subunit alpha-2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091179.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATCA TCCATGAGGA TGGCTACTCA GAGGAGGAGT GCCGGCAGTA CAAAGCTGTG 
GTCTACAGCA ACACCATCCA GTCCATCATG GCCATCATCA AGGCGATGGG GAACCTGCAG 
ATTGACTTTG GAGACTCCTC CAGAGCGGAT GACGCTCGGC AGCTCTTTGC GCTCTCCTGC 
ACTGCTGAGG AGCAGGGCAT CATGCCGGAG GATCTGGCCA ACGTGATCCG GAGGCTGTGG 
GCTGACAACG GGGTCCAGGC TTGTTTTAAC CGCTCCCGAG AGTACCAGCT GAACGACTCT 
GCTGCCTACT ACCTGAACGA CCTGGAGAGG ATAGCCCGTG CCGACTACAT CCCCACCCAG 
CAGGACGTGC TGCGCACCAG GGTGAAGACC ACCGGCATCG TGGAGACCCA CTTCACCTTC 
AAAGACCTGC ACTTCAAGAT GTTTGACGTG GGCGGCCAGC GCTCAGAGCG GAAGAAGTGG 
ATCCACTGCT TCGAGGGGGT GACGGCCATC ATTTTCTGCG TGGCCCTCAG TGCCTACGAC 
CTGGTGCTGG CTGAAGACGA GGAGATGAAC CGCATGCACG AGAGCATGAA GCTGTTCGAC 
AGCATCTGCA ACAACAAGTG GTTCACAGAC ACGTCCATCA TCCTCTTCCT CAACAAGAAG 
GACCTCTTTG AGGAGAAGAT CGTGCACAGC CCCCTGACCA TTTGCTTCCC TGAGTACACA 
GGGGCCAACA AGTATGACGA GGCGGCCAGC TACATCCAGA GCAAGTTCGA GGACCTGAAC 
AAGCGGAAGG ACACCAAGGA GATCTACACC CACTTCACCT GCGCCACTGA CACCAAGAAC 
GTGCAGTTCG TCTTTGACGC CGTCACCGAC GTCATCATCA AAAACAACCT GAAGGACTGC 
GGGCTCTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014814931.1
CDS	138..1049
Translation	MKIIHEDGYSEEECRQYKAVVYSNTIQSIMAIIKAMGNLQIDFGDSSRADDARQLFALSCTAEEQGIMPEDLANVIRRLWADNGVQACFNRSREYQLNDSAAYYLNDLERIARADYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSAYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIVHSPLTICFPEYTGANKYDEAASYIQSKFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF

Target ORF information:

RefSeq Version	XM_014959445.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 (GNAI2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014959445.1

ORF Insert Sequence:

ATGAAGATCA TCCATGAGGA TGGCTACTCA GAGGAGGAGT GCCGGCAGTA CAAAGCTGTG 
GTCTACAGCA ACACCATCCA GTCCATCATG GCCATCATCA AGGCGATGGG GAACCTGCAG 
ATTGACTTTG GAGACTCCTC CAGAGCGGAT GACGCTCGGC AGCTCTTTGC GCTCTCCTGC 
ACTGCTGAGG AGCAGGGCAT CATGCCGGAG GATCTGGCCA ACGTGATCCG GAGGCTGTGG 
GCTGACAACG GGGTCCAGGC TTGTTTTAAC CGCTCCCGAG AGTACCAGCT GAACGACTCT 
GCTGCCTACT ACCTGAACGA CCTGGAGAGG ATAGCCCGTG CCGACTACAT CCCCACCCAG 
CAGGACGTGC TGCGCACCAG GGTGAAGACC ACCGGCATCG TGGAGACCCA CTTCACCTTC 
AAAGACCTGC ACTTCAAGAT GTTTGACGTG GGCGGCCAGC GCTCAGAGCG GAAGAAGTGG 
ATCCACTGCT TCGAGGGGGT GACGGCCATC ATTTTCTGCG TGGCCCTCAG TGCCTACGAC 
CTGGTGCTGG CTGAAGACGA GGAGATGAAC CGCATGCACG AGAGCATGAA GCTGTTCGAC 
AGCATCTGCA ACAACAAGTG GTTCACAGAC ACGTCCATCA TCCTCTTCCT CAACAAGAAG 
GACCTCTTTG AGGAGAAGAT CGTGCACAGC CCCCTGACCA TTTGCTTCCC TGAGTACACA 
GGGGCCAACA AGTATGACGA GGCGGCCAGC TACATCCAGA GCAAGTTCGA GGACCTGAAC 
AAGCGGAAGG ACACCAAGGA GATCTACACC CACTTCACCT GCGCCACTGA CACCAAGAAC 
GTGCAGTTCG TCTTTGACGC CGTCACCGAC GTCATCATCA AAAACAACCT GAAGGACTGC 
GGGCTCTTCT GA