RAPGEF4 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	RAPGEF4
Entrez Gene ID	106895302
Full Name	Rap guanine nucleotide exchange factor 4
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014954656.1	XP_014810142.1	rap guanine nucleotide exchange factor 4 isoform X2
XM_014954655.1	XP_014810141.1	rap guanine nucleotide exchange factor 4 isoform X1

Pathways from BioSystems
Gene Ontology

The following RAPGEF4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAPGEF4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa74295	XM_014954655.1 Latest version!	Calidris pugnax Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OCa74296	XM_014954656.1 Latest version!	Calidris pugnax Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RAPGEF4 ( XM_014954655.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014810141.1 Calidris pugnax Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCa74295
Clone ID Related Accession (Same CDS sequence)	XM_014954655.1
Accession Version	XM_014954655.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2622bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	rap guanine nucleotide exchange factor 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090990.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAC TTCTTACTCC TCCTTATGGT GTTATGGAAA CTGGCTCCAA CAATGACAGA 
ATGCCAGATA AGGATGGTAT GTCAAGCGGT GCACTTTGCC AAGTTTCTAA AAATTGTAAT 
AAGACACCTC TTATTGAACC TCACATCCCA CATCGTCCTA CTAAAACCAT TACGCAGGTT 
CCTTCAGAAA AGATCCTCAG AGCTGGAAAG ATTTTGCGGA ATACAATTCT GTCCCGAGCC 
CCTCACATGA TAAGAGATCG TAAATACCAT CTGAAGACTT ACAGGCAATG CTGTGTGGGG 
ACAGAACTAG TTGACTGGAT GATGCAGCAA AGTCCTTGTG TCCATTCACG AACCCAGGCT 
GTTGGCATGT GGCAAGTATT ACTGGAAGAA GGTGTTCTTA ACCATGTGGA TCAGGAACAC 
TACTTTCAAG ATAAATACTT ATTTTATCGA TTTTTGGATG ATGAGCGTGA AGATGCCCCT 
TTGCCAACTG AGGAAGAGAA AAAAGAATGT GATGAGGAGC TACAGGACAC TATGCTGCTT 
CTGTCTCAGA TTGGGCCAGA TGCTCATATG AGGATGATTC TCAGAAAACC GCCTGGCCAG 
AGAACTGTAG ATGACTTGGA AATTATATAT GAAGAACTTC TTCACATTAA GGCCTTATCT 
CATCTTTCTA CCACAGTAAA GCGAGAATTA GCAGGTGTCC TCATTTTCGA GTCACATCCC 
AAAGCAGGAA CGGTCTTATT TAATCAGGGA GAAGAAGGTA CTTCTTGGTA CATTATCCTA 
AAGGGATCAG TAAACGTAGT CATTTATGGC AAGGGTGTGG TATGTACTCT ACACGAAGGA 
GATGATTTTG GCAAGTTAGC GCTTGTGAAT GATGCTCCCA GAGCAGCATC CATTGTTCTG 
CGTGAAGACA ACTGCCACTT CTTGAGAGTA GACAAGGAGG ACTTCAACAG GATCCTTAGG 
GATGTGGAAG CAAATACAGT AAGACTCAAA GAACATGACC AAGATGTCTT GGTGTTGGAG 
AAGATCCCAG CAGGAAACAG AGTTTCTAAT CAAGGAAATT CACAGCCTCA GCACAAGTAT 
ATTGTGATGT CAGGAACCCC AGAGAAAATT TTAGAGCACT TTCTAGAAAC TATGCGCCTT 
GAAGCAACTT TAAATGAAGC AACAGATTCT GTTTTAAATG ATTTTATTAT GATGCACTGT 
GTTTTTATGC CAAATAGTCA GCTCTGCCCA GCCTTGATGG CACATTACCA TGCCCAGCCT 
TCCCAGGGTA CAGAGCAGGA GAAAATGGAT TATGCCCTCA ACAATAAAAG GCGAGTCATT 
CGACTGGTTC TGCAGTGGGC TGCTTTATAT GGAGATTTAC TGCAAGAAGA TGAAGCGGCA 
ATGGCCTTTC TGGAGGAATT TTATGTATCT GTATCAGATG ATACTAGAAT GATTGCAGCG 
CTAAAGGAGC AACTTCCAGA GCTAGAGAAA ATTGTAAAGC AAATTTCTGA AGAACCTAAA 
GCAGCACAAA AGAAGCACAA AGTTCTTCTG CAGCTGTTCA ACACTGGTGA TGATAGAGCA 
CAGAAACGCC AGCCTATCAG GGGCAGTGAT GAAGTTTTAT TTAAGGTGTA CTGCATAGAC 
CAAACCTATA CCACTATCCG GGTGCCAGTG TCTTCCTCTG TGAAGGAAGT GATCAGCGCA 
GTAGCAGATA AGCTGGGTTC TGGAGAAGGC CTCATCATAG TAAAGATGAG TTCAGGAGGA 
GAAAAGGTTG TGCTCAAACC TCATGATGTT TCAGTGTTTA CAACTCTCAG TGTCAATGGA 
CGGCTGTTTG CTTGCCCACG TGATCAGTTC GATTCATTGG CACCATTACC AGAACAAGAA 
GGACCATCTA CTGGTACAGT GGGAACATTT GAACTGATGA GCTCCAAAGA CTTAGCACAT 
CAGATGACAA TTTATGACTG GGAGCTTTTC AACTGTGTGC ATGAGCTGGA ACTGATCTAT 
CACACTTTTG GAAGGCACAA TTTTAAAAAG ACCACAGCAA ATCTGGACTT GTTTTTAAGA 
AGATTTAATG AAATTCAGTT CTGGGTGGTC ACTGAGATTT GTCTTTGCTC TCAACTTAGC 
AAGCGTGTTC AGCTGTTAAA GAAGTATATT AAGATAGCAG CCCACTGTAA AGAATACAAA 
AATCTGAATT CCTTTTTCGC TATTATTATG GGACTGAGTA ATGTTGCTGT GAGTCGTCTC 
TCATTAACAT GGGAGAAACT TCCAAGCAAG TTCAAGAAGA TCTATGCAGA GTTTGAAAGC 
TTAATGGATC CATCAAGAAA CCATCGGGCC TACAGACTAA CGGTAGCTAA ATTGGACCCT 
CCAATAATTC CTTTCATGCC ACTACTTATA AAAGACATGA CGTTTACTCA TGAGGGAAAC 
AAGACATTCA CCGACAATCT AGTAAACTTT GAAAAAATGC GCATGATTGC CAACACTGTC 
AGGACGGTGA AATTCTGCCG AAGCCAATCT TTCAATCCCG ACGCAGCCCT AGCTAATAAG 
AACCATCAGG ATGTTCGGAG CTATGTGCGG CAATTAAATG TGATTGACAA CCAGAGAACT 
TTATCACAGA TGTCTCACCG ACTAGAACCG CGTCGAGCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014810141.1
CDS	211..2832
Translation	MSGLLTPPYGVMETGSNNDRMPDKDGMSSGALCQVSKNCNKTPLIEPHIPHRPTKTITQVPSEKILRAGKILRNTILSRAPHMIRDRKYHLKTYRQCCVGTELVDWMMQQSPCVHSRTQAVGMWQVLLEEGVLNHVDQEHYFQDKYLFYRFLDDEREDAPLPTEEEKKECDEELQDTMLLLSQIGPDAHMRMILRKPPGQRTVDDLEIIYEELLHIKALSHLSTTVKRELAGVLIFESHPKAGTVLFNQGEEGTSWYIILKGSVNVVIYGKGVVCTLHEGDDFGKLALVNDAPRAASIVLREDNCHFLRVDKEDFNRILRDVEANTVRLKEHDQDVLVLEKIPAGNRVSNQGNSQPQHKYIVMSGTPEKILEHFLETMRLEATLNEATDSVLNDFIMMHCVFMPNSQLCPALMAHYHAQPSQGTEQEKMDYALNNKRRVIRLVLQWAALYGDLLQEDEAAMAFLEEFYVSVSDDTRMIAALKEQLPELEKIVKQISEEPKAAQKKHKVLLQLFNTGDDRAQKRQPIRGSDEVLFKVYCIDQTYTTIRVPVSSSVKEVISAVADKLGSGEGLIIVKMSSGGEKVVLKPHDVSVFTTLSVNGRLFACPRDQFDSLAPLPEQEGPSTGTVGTFELMSSKDLAHQMTIYDWELFNCVHELELIYHTFGRHNFKKTTANLDLFLRRFNEIQFWVVTEICLCSQLSKRVQLLKKYIKIAAHCKEYKNLNSFFAIIMGLSNVAVSRLSLTWEKLPSKFKKIYAEFESLMDPSRNHRAYRLTVAKLDPPIIPFMPLLIKDMTFTHEGNKTFTDNLVNFEKMRMIANTVRTVKFCRSQSFNPDAALANKNHQDVRSYVRQLNVIDNQRTLSQMSHRLEPRRA

Target ORF information:

RefSeq Version	XM_014954655.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014954655.1

ORF Insert Sequence:

ATGTCTGGAC TTCTTACTCC TCCTTATGGT GTTATGGAAA CTGGCTCCAA CAATGACAGA 
ATGCCAGATA AGGATGGTAT GTCAAGCGGT GCACTTTGCC AAGTTTCTAA AAATTGTAAT 
AAGACACCTC TTATTGAACC TCACATCCCA CATCGTCCTA CTAAAACCAT TACGCAGGTT 
CCTTCAGAAA AGATCCTCAG AGCTGGAAAG ATTTTGCGGA ATACAATTCT GTCCCGAGCC 
CCTCACATGA TAAGAGATCG TAAATACCAT CTGAAGACTT ACAGGCAATG CTGTGTGGGG 
ACAGAACTAG TTGACTGGAT GATGCAGCAA AGTCCTTGTG TCCATTCACG AACCCAGGCT 
GTTGGCATGT GGCAAGTATT ACTGGAAGAA GGTGTTCTTA ACCATGTGGA TCAGGAACAC 
TACTTTCAAG ATAAATACTT ATTTTATCGA TTTTTGGATG ATGAGCGTGA AGATGCCCCT 
TTGCCAACTG AGGAAGAGAA AAAAGAATGT GATGAGGAGC TACAGGACAC TATGCTGCTT 
CTGTCTCAGA TTGGGCCAGA TGCTCATATG AGGATGATTC TCAGAAAACC GCCTGGCCAG 
AGAACTGTAG ATGACTTGGA AATTATATAT GAAGAACTTC TTCACATTAA GGCCTTATCT 
CATCTTTCTA CCACAGTAAA GCGAGAATTA GCAGGTGTCC TCATTTTCGA GTCACATCCC 
AAAGCAGGAA CGGTCTTATT TAATCAGGGA GAAGAAGGTA CTTCTTGGTA CATTATCCTA 
AAGGGATCAG TAAACGTAGT CATTTATGGC AAGGGTGTGG TATGTACTCT ACACGAAGGA 
GATGATTTTG GCAAGTTAGC GCTTGTGAAT GATGCTCCCA GAGCAGCATC CATTGTTCTG 
CGTGAAGACA ACTGCCACTT CTTGAGAGTA GACAAGGAGG ACTTCAACAG GATCCTTAGG 
GATGTGGAAG CAAATACAGT AAGACTCAAA GAACATGACC AAGATGTCTT GGTGTTGGAG 
AAGATCCCAG CAGGAAACAG AGTTTCTAAT CAAGGAAATT CACAGCCTCA GCACAAGTAT 
ATTGTGATGT CAGGAACCCC AGAGAAAATT TTAGAGCACT TTCTAGAAAC TATGCGCCTT 
GAAGCAACTT TAAATGAAGC AACAGATTCT GTTTTAAATG ATTTTATTAT GATGCACTGT 
GTTTTTATGC CAAATAGTCA GCTCTGCCCA GCCTTGATGG CACATTACCA TGCCCAGCCT 
TCCCAGGGTA CAGAGCAGGA GAAAATGGAT TATGCCCTCA ACAATAAAAG GCGAGTCATT 
CGACTGGTTC TGCAGTGGGC TGCTTTATAT GGAGATTTAC TGCAAGAAGA TGAAGCGGCA 
ATGGCCTTTC TGGAGGAATT TTATGTATCT GTATCAGATG ATACTAGAAT GATTGCAGCG 
CTAAAGGAGC AACTTCCAGA GCTAGAGAAA ATTGTAAAGC AAATTTCTGA AGAACCTAAA 
GCAGCACAAA AGAAGCACAA AGTTCTTCTG CAGCTGTTCA ACACTGGTGA TGATAGAGCA 
CAGAAACGCC AGCCTATCAG GGGCAGTGAT GAAGTTTTAT TTAAGGTGTA CTGCATAGAC 
CAAACCTATA CCACTATCCG GGTGCCAGTG TCTTCCTCTG TGAAGGAAGT GATCAGCGCA 
GTAGCAGATA AGCTGGGTTC TGGAGAAGGC CTCATCATAG TAAAGATGAG TTCAGGAGGA 
GAAAAGGTTG TGCTCAAACC TCATGATGTT TCAGTGTTTA CAACTCTCAG TGTCAATGGA 
CGGCTGTTTG CTTGCCCACG TGATCAGTTC GATTCATTGG CACCATTACC AGAACAAGAA 
GGACCATCTA CTGGTACAGT GGGAACATTT GAACTGATGA GCTCCAAAGA CTTAGCACAT 
CAGATGACAA TTTATGACTG GGAGCTTTTC AACTGTGTGC ATGAGCTGGA ACTGATCTAT 
CACACTTTTG GAAGGCACAA TTTTAAAAAG ACCACAGCAA ATCTGGACTT GTTTTTAAGA 
AGATTTAATG AAATTCAGTT CTGGGTGGTC ACTGAGATTT GTCTTTGCTC TCAACTTAGC 
AAGCGTGTTC AGCTGTTAAA GAAGTATATT AAGATAGCAG CCCACTGTAA AGAATACAAA 
AATCTGAATT CCTTTTTCGC TATTATTATG GGACTGAGTA ATGTTGCTGT GAGTCGTCTC 
TCATTAACAT GGGAGAAACT TCCAAGCAAG TTCAAGAAGA TCTATGCAGA GTTTGAAAGC 
TTAATGGATC CATCAAGAAA CCATCGGGCC TACAGACTAA CGGTAGCTAA ATTGGACCCT 
CCAATAATTC CTTTCATGCC ACTACTTATA AAAGACATGA CGTTTACTCA TGAGGGAAAC 
AAGACATTCA CCGACAATCT AGTAAACTTT GAAAAAATGC GCATGATTGC CAACACTGTC 
AGGACGGTGA AATTCTGCCG AAGCCAATCT TTCAATCCCG ACGCAGCCCT AGCTAATAAG 
AACCATCAGG ATGTTCGGAG CTATGTGCGG CAATTAAATG TGATTGACAA CCAGAGAACT 
TTATCACAGA TGTCTCACCG ACTAGAACCG CGTCGAGCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF4 ( XM_014954656.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014810142.1 Calidris pugnax Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCa74296
Clone ID Related Accession (Same CDS sequence)	XM_014954656.1
Accession Version	XM_014954656.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	rap guanine nucleotide exchange factor 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090990.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAC TTCTTACTCC TCCTTATGGT GTTATGGAAA CTGGCTCCAA CAATGACAGA 
ATGCCAGATA AGGATGGTAT GTCAAGCGGT GCACTTTGCC AAGTTTCTAA AAATTGTAAT 
AAGGTTCCTT CAGAAAAGAT CCTCAGAGCT GGAAAGATTT TGCGGAATAC AATTCTGTCC 
CGAGCCCCTC ACATGATAAG AGATCGTAAA TACCATCTGA AGACTTACAG GCAATGCTGT 
GTGGGGACAG AACTAGTTGA CTGGATGATG CAGCAAAGTC CTTGTGTCCA TTCACGAACC 
CAGGCTGTTG GCATGTGGCA AGTATTACTG GAAGAAGGTG TTCTTAACCA TGTGGATCAG 
GAACACTACT TTCAAGATAA ATACTTATTT TATCGATTTT TGGATGATGA GCGTGAAGAT 
GCCCCTTTGC CAACTGAGGA AGAGAAAAAA GAATGTGATG AGGAGCTACA GGACACTATG 
CTGCTTCTGT CTCAGATTGG GCCAGATGCT CATATGAGGA TGATTCTCAG AAAACCGCCT 
GGCCAGAGAA CTGTAGATGA CTTGGAAATT ATATATGAAG AACTTCTTCA CATTAAGGCC 
TTATCTCATC TTTCTACCAC AGTAAAGCGA GAATTAGCAG GTGTCCTCAT TTTCGAGTCA 
CATCCCAAAG CAGGAACGGT CTTATTTAAT CAGGGAGAAG AAGGTACTTC TTGGTACATT 
ATCCTAAAGG GATCAGTAAA CGTAGTCATT TATGGCAAGG GTGTGGTATG TACTCTACAC 
GAAGGAGATG ATTTTGGCAA GTTAGCGCTT GTGAATGATG CTCCCAGAGC AGCATCCATT 
GTTCTGCGTG AAGACAACTG CCACTTCTTG AGAGTAGACA AGGAGGACTT CAACAGGATC 
CTTAGGGATG TGGAAGCAAA TACAGTAAGA CTCAAAGAAC ATGACCAAGA TGTCTTGGTG 
TTGGAGAAGA TCCCAGCAGG AAACAGAGTT TCTAATCAAG GAAATTCACA GCCTCAGCAC 
AAGTATATTG TGATGTCAGG AACCCCAGAG AAAATTTTAG AGCACTTTCT AGAAACTATG 
CGCCTTGAAG CAACTTTAAA TGAAGCAACA GATTCTGTTT TAAATGATTT TATTATGATG 
CACTGTGTTT TTATGCCAAA TAGTCAGCTC TGCCCAGCCT TGATGGCACA TTACCATGCC 
CAGCCTTCCC AGGGTACAGA GCAGGAGAAA ATGGATTATG CCCTCAACAA TAAAAGGCGA 
GTCATTCGAC TGGTTCTGCA GTGGGCTGCT TTATATGGAG ATTTACTGCA AGAAGATGAA 
GCGGCAATGG CCTTTCTGGA GGAATTTTAT GTATCTGTAT CAGATGATAC TAGAATGATT 
GCAGCGCTAA AGGAGCAACT TCCAGAGCTA GAGAAAATTG TAAAGCAAAT TTCTGAAGAA 
CCTAAAGCAG CACAAAAGAA GCACAAAGTT CTTCTGCAGC TGTTCAACAC TGGTGATGAT 
AGAGCACAGA AACGCCAGCC TATCAGGGGC AGTGATGAAG TTTTATTTAA GGTGTACTGC 
ATAGACCAAA CCTATACCAC TATCCGGGTG CCAGTGTCTT CCTCTGTGAA GGAAGTGATC 
AGCGCAGTAG CAGATAAGCT GGGTTCTGGA GAAGGCCTCA TCATAGTAAA GATGAGTTCA 
GGAGGAGAAA AGGTTGTGCT CAAACCTCAT GATGTTTCAG TGTTTACAAC TCTCAGTGTC 
AATGGACGGC TGTTTGCTTG CCCACGTGAT CAGTTCGATT CATTGGCACC ATTACCAGAA 
CAAGAAGGAC CATCTACTGG TACAGTGGGA ACATTTGAAC TGATGAGCTC CAAAGACTTA 
GCACATCAGA TGACAATTTA TGACTGGGAG CTTTTCAACT GTGTGCATGA GCTGGAACTG 
ATCTATCACA CTTTTGGAAG GCACAATTTT AAAAAGACCA CAGCAAATCT GGACTTGTTT 
TTAAGAAGAT TTAATGAAAT TCAGTTCTGG GTGGTCACTG AGATTTGTCT TTGCTCTCAA 
CTTAGCAAGC GTGTTCAGCT GTTAAAGAAG TATATTAAGA TAGCAGCCCA CTGTAAAGAA 
TACAAAAATC TGAATTCCTT TTTCGCTATT ATTATGGGAC TGAGTAATGT TGCTGTGAGT 
CGTCTCTCAT TAACATGGGA GAAACTTCCA AGCAAGTTCA AGAAGATCTA TGCAGAGTTT 
GAAAGCTTAA TGGATCCATC AAGAAACCAT CGGGCCTACA GACTAACGGT AGCTAAATTG 
GACCCTCCAA TAATTCCTTT CATGCCACTA CTTATAAAAG ACATGACGTT TACTCATGAG 
GGAAACAAGA CATTCACCGA CAATCTAGTA AACTTTGAAA AAATGCGCAT GATTGCCAAC 
ACTGTCAGGA CGGTGAAATT CTGCCGAAGC CAATCTTTCA ATCCCGACGC AGCCCTAGCT 
AATAAGAACC ATCAGGATGT TCGGAGCTAT GTGCGGCAAT TAAATGTGAT TGACAACCAG 
AGAACTTTAT CACAGATGTC TCACCGACTA GAACCGCGTC GAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014810142.1
CDS	391..2958
Translation	MSGLLTPPYGVMETGSNNDRMPDKDGMSSGALCQVSKNCNKVPSEKILRAGKILRNTILSRAPHMIRDRKYHLKTYRQCCVGTELVDWMMQQSPCVHSRTQAVGMWQVLLEEGVLNHVDQEHYFQDKYLFYRFLDDEREDAPLPTEEEKKECDEELQDTMLLLSQIGPDAHMRMILRKPPGQRTVDDLEIIYEELLHIKALSHLSTTVKRELAGVLIFESHPKAGTVLFNQGEEGTSWYIILKGSVNVVIYGKGVVCTLHEGDDFGKLALVNDAPRAASIVLREDNCHFLRVDKEDFNRILRDVEANTVRLKEHDQDVLVLEKIPAGNRVSNQGNSQPQHKYIVMSGTPEKILEHFLETMRLEATLNEATDSVLNDFIMMHCVFMPNSQLCPALMAHYHAQPSQGTEQEKMDYALNNKRRVIRLVLQWAALYGDLLQEDEAAMAFLEEFYVSVSDDTRMIAALKEQLPELEKIVKQISEEPKAAQKKHKVLLQLFNTGDDRAQKRQPIRGSDEVLFKVYCIDQTYTTIRVPVSSSVKEVISAVADKLGSGEGLIIVKMSSGGEKVVLKPHDVSVFTTLSVNGRLFACPRDQFDSLAPLPEQEGPSTGTVGTFELMSSKDLAHQMTIYDWELFNCVHELELIYHTFGRHNFKKTTANLDLFLRRFNEIQFWVVTEICLCSQLSKRVQLLKKYIKIAAHCKEYKNLNSFFAIIMGLSNVAVSRLSLTWEKLPSKFKKIYAEFESLMDPSRNHRAYRLTVAKLDPPIIPFMPLLIKDMTFTHEGNKTFTDNLVNFEKMRMIANTVRTVKFCRSQSFNPDAALANKNHQDVRSYVRQLNVIDNQRTLSQMSHRLEPRRA

Target ORF information:

RefSeq Version	XM_014954656.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014954656.1

ORF Insert Sequence:

ATGTCTGGAC TTCTTACTCC TCCTTATGGT GTTATGGAAA CTGGCTCCAA CAATGACAGA 
ATGCCAGATA AGGATGGTAT GTCAAGCGGT GCACTTTGCC AAGTTTCTAA AAATTGTAAT 
AAGGTTCCTT CAGAAAAGAT CCTCAGAGCT GGAAAGATTT TGCGGAATAC AATTCTGTCC 
CGAGCCCCTC ACATGATAAG AGATCGTAAA TACCATCTGA AGACTTACAG GCAATGCTGT 
GTGGGGACAG AACTAGTTGA CTGGATGATG CAGCAAAGTC CTTGTGTCCA TTCACGAACC 
CAGGCTGTTG GCATGTGGCA AGTATTACTG GAAGAAGGTG TTCTTAACCA TGTGGATCAG 
GAACACTACT TTCAAGATAA ATACTTATTT TATCGATTTT TGGATGATGA GCGTGAAGAT 
GCCCCTTTGC CAACTGAGGA AGAGAAAAAA GAATGTGATG AGGAGCTACA GGACACTATG 
CTGCTTCTGT CTCAGATTGG GCCAGATGCT CATATGAGGA TGATTCTCAG AAAACCGCCT 
GGCCAGAGAA CTGTAGATGA CTTGGAAATT ATATATGAAG AACTTCTTCA CATTAAGGCC 
TTATCTCATC TTTCTACCAC AGTAAAGCGA GAATTAGCAG GTGTCCTCAT TTTCGAGTCA 
CATCCCAAAG CAGGAACGGT CTTATTTAAT CAGGGAGAAG AAGGTACTTC TTGGTACATT 
ATCCTAAAGG GATCAGTAAA CGTAGTCATT TATGGCAAGG GTGTGGTATG TACTCTACAC 
GAAGGAGATG ATTTTGGCAA GTTAGCGCTT GTGAATGATG CTCCCAGAGC AGCATCCATT 
GTTCTGCGTG AAGACAACTG CCACTTCTTG AGAGTAGACA AGGAGGACTT CAACAGGATC 
CTTAGGGATG TGGAAGCAAA TACAGTAAGA CTCAAAGAAC ATGACCAAGA TGTCTTGGTG 
TTGGAGAAGA TCCCAGCAGG AAACAGAGTT TCTAATCAAG GAAATTCACA GCCTCAGCAC 
AAGTATATTG TGATGTCAGG AACCCCAGAG AAAATTTTAG AGCACTTTCT AGAAACTATG 
CGCCTTGAAG CAACTTTAAA TGAAGCAACA GATTCTGTTT TAAATGATTT TATTATGATG 
CACTGTGTTT TTATGCCAAA TAGTCAGCTC TGCCCAGCCT TGATGGCACA TTACCATGCC 
CAGCCTTCCC AGGGTACAGA GCAGGAGAAA ATGGATTATG CCCTCAACAA TAAAAGGCGA 
GTCATTCGAC TGGTTCTGCA GTGGGCTGCT TTATATGGAG ATTTACTGCA AGAAGATGAA 
GCGGCAATGG CCTTTCTGGA GGAATTTTAT GTATCTGTAT CAGATGATAC TAGAATGATT 
GCAGCGCTAA AGGAGCAACT TCCAGAGCTA GAGAAAATTG TAAAGCAAAT TTCTGAAGAA 
CCTAAAGCAG CACAAAAGAA GCACAAAGTT CTTCTGCAGC TGTTCAACAC TGGTGATGAT 
AGAGCACAGA AACGCCAGCC TATCAGGGGC AGTGATGAAG TTTTATTTAA GGTGTACTGC 
ATAGACCAAA CCTATACCAC TATCCGGGTG CCAGTGTCTT CCTCTGTGAA GGAAGTGATC 
AGCGCAGTAG CAGATAAGCT GGGTTCTGGA GAAGGCCTCA TCATAGTAAA GATGAGTTCA 
GGAGGAGAAA AGGTTGTGCT CAAACCTCAT GATGTTTCAG TGTTTACAAC TCTCAGTGTC 
AATGGACGGC TGTTTGCTTG CCCACGTGAT CAGTTCGATT CATTGGCACC ATTACCAGAA 
CAAGAAGGAC CATCTACTGG TACAGTGGGA ACATTTGAAC TGATGAGCTC CAAAGACTTA 
GCACATCAGA TGACAATTTA TGACTGGGAG CTTTTCAACT GTGTGCATGA GCTGGAACTG 
ATCTATCACA CTTTTGGAAG GCACAATTTT AAAAAGACCA CAGCAAATCT GGACTTGTTT 
TTAAGAAGAT TTAATGAAAT TCAGTTCTGG GTGGTCACTG AGATTTGTCT TTGCTCTCAA 
CTTAGCAAGC GTGTTCAGCT GTTAAAGAAG TATATTAAGA TAGCAGCCCA CTGTAAAGAA 
TACAAAAATC TGAATTCCTT TTTCGCTATT ATTATGGGAC TGAGTAATGT TGCTGTGAGT 
CGTCTCTCAT TAACATGGGA GAAACTTCCA AGCAAGTTCA AGAAGATCTA TGCAGAGTTT 
GAAAGCTTAA TGGATCCATC AAGAAACCAT CGGGCCTACA GACTAACGGT AGCTAAATTG 
GACCCTCCAA TAATTCCTTT CATGCCACTA CTTATAAAAG ACATGACGTT TACTCATGAG 
GGAAACAAGA CATTCACCGA CAATCTAGTA AACTTTGAAA AAATGCGCAT GATTGCCAAC 
ACTGTCAGGA CGGTGAAATT CTGCCGAAGC CAATCTTTCA ATCCCGACGC AGCCCTAGCT 
AATAAGAACC ATCAGGATGT TCGGAGCTAT GTGCGGCAAT TAAATGTGAT TGACAACCAG 
AGAACTTTAT CACAGATGTC TCACCGACTA GAACCGCGTC GAGCATAA