CCDC77 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	CCDC77
Entrez Gene ID	106885206
Full Name	coiled-coil domain containing 77
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014937198.1	XP_014792684.1	coiled-coil domain-containing protein 77

Pathways from BioSystems
Gene Ontology

The following CCDC77 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CCDC77 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa61530	XM_014937198.1 Latest version!	Calidris pugnax coiled-coil domain containing 77 (CCDC77), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CCDC77 ( XM_014937198.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014792684.1 Calidris pugnax coiled-coil domain containing 77 (CCDC77), mRNA.

Price & Availability↑

CloneID	OCa61530
Clone ID Related Accession (Same CDS sequence)	XM_014937198.1
Accession Version	XM_014937198.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	coiled-coil domain-containing protein 77
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090800.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGCCA CGGACCGCTC TGCGGGGACC CCCGCTCGCT CCAGAAGGCT GGCAACCCCC 
CATGTCTCCT CCAGATATGC TAGTTCTCAG CCTCCAAATC AGGAGGACTT TACTCCTCTC 
CCTTCAATCA ATGAGCGTTT GGCCCTTCTC CGTCCTTCCC GGGAGCTGCT GGAATACTAC 
CGTAAGAAGA TTGCTGACTT TGATGAGGAA TATGAAGATT TGGTAAAAAG GCTGGAGAGA 
TACAAAGAAA CGTATGATGA GCAGCACAAA TTGCAGCAGG AGGTGCATCA GCGGGAAGAG 
GAGATTGCGG AGTTGCAAAA GGCTTTGAGT GATATGCAAG TCTACCTCTT CCAGGAGAGG 
GAACATGTCC TTCGTCTTTA CTCGGAGAAT GACCGGCTGA AAATCAGGGA ATTGGAGGAC 
AGAAAAAAAA TTCAACATCT CCTGGCTTTA GTGGGAACAG ACAAAGGAGA AGTTACATAT 
TTTCACAAGG AGCCTCCACA TAAGGTTACT GTTCTGCAAA GGCCGGCTAA ATCCAGAGAT 
CCACATGAAC GAAATGATAC TTCTAGAACA GGTGCCAAGA GAAGCACTTC AAAAACAGCA 
GCAAAAGGAG AGAAACCAGA AAGTCCTGAG AGATATCAGA GAGACAATCA AACACTCCTA 
CTTCAGGTGG AGGCCCTGCA AGCTCAGATA GAAGAACAGA CACGATTATC CAAGGAACAG 
GTTGAGGCAC TACTAGAGGA CAGGCGGATT CACATGGAGG AAGCCCAGGT CCAGCATCAG 
AGGGACCAGG CCAAGATCAA AACTATAACA GATAAACTCC ATAAGACCCA AAATCTCTTA 
TATGAGAGTA CCCGTGACTT TCTCCAGCTC AAGTTTGATG CCCGAGCCAA TGAGAAAGCA 
TGGATGGCAG AGAAGGACAG TCTGTTGAGG AAACTTGACA AAGATTCGGA TCAGCTTATT 
ATCAGCAAGG AACCAGGAAG AGAGAAGAAG CAGAGAGATA CCAAGAAGAA GCTTCAAGCA 
GATAATGGGG CTTGGAAACT CCACAGCAGA GAAATAAAGT CACTGCAAGA ACAGCTAATG 
CAGGAACAAC GCCTATCAAA CATGTATAGA GAGCAGTGTG TCACCCTGGA AGATGAGCTG 
GCTAGGATTC GTGAGGAGGG AGATGTTGGC AGAGAACTCT TTAAGGAACG CTCAGAAAAG 
ATGGGGAAGC GCCTCAAGCT GGTGACTCAG CGATATGAGG CCTTGGAAAA ACGTCGTAAT 
ATGGAAGTGG AAGGCTTCAA GAATGACATT AAGCAGCTTC GGCAGAAGCT GAGAGATGTA 
GAGGACCAGC TTTTTAAGGT GACTTTGAAT ATTGGACCAG ACCAGGATCT TGCAATTCTA 
CGTGAGGTCC ACCAAGGAAA CAGACTAACC CGTAAAATTC AAGGAGAACT AAAAAATTTA 
AAAGCTAAAA TCTATGGCTT AGAGAATGAA CTACGAGTCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014792684.1
CDS	226..1710
Translation	MSATDRSAGTPARSRRLATPHVSSRYASSQPPNQEDFTPLPSINERLALLRPSRELLEYYRKKIADFDEEYEDLVKRLERYKETYDEQHKLQQEVHQREEEIAELQKALSDMQVYLFQEREHVLRLYSENDRLKIRELEDRKKIQHLLALVGTDKGEVTYFHKEPPHKVTVLQRPAKSRDPHERNDTSRTGAKRSTSKTAAKGEKPESPERYQRDNQTLLLQVEALQAQIEEQTRLSKEQVEALLEDRRIHMEEAQVQHQRDQAKIKTITDKLHKTQNLLYESTRDFLQLKFDARANEKAWMAEKDSLLRKLDKDSDQLIISKEPGREKKQRDTKKKLQADNGAWKLHSREIKSLQEQLMQEQRLSNMYREQCVTLEDELARIREEGDVGRELFKERSEKMGKRLKLVTQRYEALEKRRNMEVEGFKNDIKQLRQKLRDVEDQLFKVTLNIGPDQDLAILREVHQGNRLTRKIQGELKNLKAKIYGLENELRVC

Target ORF information:

RefSeq Version	XM_014937198.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax coiled-coil domain containing 77 (CCDC77), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014937198.1

ORF Insert Sequence:

ATGAGCGCCA CGGACCGCTC TGCGGGGACC CCCGCTCGCT CCAGAAGGCT GGCAACCCCC 
CATGTCTCCT CCAGATATGC TAGTTCTCAG CCTCCAAATC AGGAGGACTT TACTCCTCTC 
CCTTCAATCA ATGAGCGTTT GGCCCTTCTC CGTCCTTCCC GGGAGCTGCT GGAATACTAC 
CGTAAGAAGA TTGCTGACTT TGATGAGGAA TATGAAGATT TGGTAAAAAG GCTGGAGAGA 
TACAAAGAAA CGTATGATGA GCAGCACAAA TTGCAGCAGG AGGTGCATCA GCGGGAAGAG 
GAGATTGCGG AGTTGCAAAA GGCTTTGAGT GATATGCAAG TCTACCTCTT CCAGGAGAGG 
GAACATGTCC TTCGTCTTTA CTCGGAGAAT GACCGGCTGA AAATCAGGGA ATTGGAGGAC 
AGAAAAAAAA TTCAACATCT CCTGGCTTTA GTGGGAACAG ACAAAGGAGA AGTTACATAT 
TTTCACAAGG AGCCTCCACA TAAGGTTACT GTTCTGCAAA GGCCGGCTAA ATCCAGAGAT 
CCACATGAAC GAAATGATAC TTCTAGAACA GGTGCCAAGA GAAGCACTTC AAAAACAGCA 
GCAAAAGGAG AGAAACCAGA AAGTCCTGAG AGATATCAGA GAGACAATCA AACACTCCTA 
CTTCAGGTGG AGGCCCTGCA AGCTCAGATA GAAGAACAGA CACGATTATC CAAGGAACAG 
GTTGAGGCAC TACTAGAGGA CAGGCGGATT CACATGGAGG AAGCCCAGGT CCAGCATCAG 
AGGGACCAGG CCAAGATCAA AACTATAACA GATAAACTCC ATAAGACCCA AAATCTCTTA 
TATGAGAGTA CCCGTGACTT TCTCCAGCTC AAGTTTGATG CCCGAGCCAA TGAGAAAGCA 
TGGATGGCAG AGAAGGACAG TCTGTTGAGG AAACTTGACA AAGATTCGGA TCAGCTTATT 
ATCAGCAAGG AACCAGGAAG AGAGAAGAAG CAGAGAGATA CCAAGAAGAA GCTTCAAGCA 
GATAATGGGG CTTGGAAACT CCACAGCAGA GAAATAAAGT CACTGCAAGA ACAGCTAATG 
CAGGAACAAC GCCTATCAAA CATGTATAGA GAGCAGTGTG TCACCCTGGA AGATGAGCTG 
GCTAGGATTC GTGAGGAGGG AGATGTTGGC AGAGAACTCT TTAAGGAACG CTCAGAAAAG 
ATGGGGAAGC GCCTCAAGCT GGTGACTCAG CGATATGAGG CCTTGGAAAA ACGTCGTAAT 
ATGGAAGTGG AAGGCTTCAA GAATGACATT AAGCAGCTTC GGCAGAAGCT GAGAGATGTA 
GAGGACCAGC TTTTTAAGGT GACTTTGAAT ATTGGACCAG ACCAGGATCT TGCAATTCTA 
CGTGAGGTCC ACCAAGGAAA CAGACTAACC CGTAAAATTC AAGGAGAACT AAAAAATTTA 
AAAGCTAAAA TCTATGGCTT AGAGAATGAA CTACGAGTCT GCTGA