ZP2 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	ZP2
Entrez Gene ID	106884673
Full Name	zona pellucida glycoprotein 2
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014936197.1	XP_014791683.1	zona pellucida sperm-binding protein 2

Pathways from BioSystems
Gene Ontology

The following ZP2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZP2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa60771	XM_014936197.1 Latest version!	Calidris pugnax zona pellucida glycoprotein 2 (sperm receptor) (ZP2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ZP2 ( XM_014936197.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014791683.1 Calidris pugnax zona pellucida glycoprotein 2 (sperm receptor) (ZP2), mRNA.

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CloneID	OCa60771
Clone ID Related Accession (Same CDS sequence)	XM_014936197.1
Accession Version	XM_014936197.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	zona pellucida sperm-binding protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090794.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGGACTGT TTGTGGAGCA GCAGTTCTTA TGCACCTCAA GGATGTGGTT GCTGCTCCTG 
TTTGGCCTTT TGCTGCCTTT GGCCCCTTGT GCTGATGGCC TGGGGGATCA GGATCTCTTG 
GAGAGTGTGA CCTGTCACAG GGACAGGATG GAAGTTGAGT TTTATGGAGA GCTTGGAAAC 
TACTCCTGGC ACGTGTGTGT TGTGGATGTG AGTGGTGAGG AGATGGTGTC CTGCAACTAC 
ACTGTGGATT ATGAGAGGCT GTTGCTCAGT GTCCTGTTTG TGAACTGCAC AAGCCTGGAG 
CATGATCAGC ACCTGCTAAG ATTGAGGCTG ATGGTAAATG ACACAATGGG AGAGGAGAAG 
AATGTAACCT ACAGCACTCA GTGCAATACT GTTCATGCAG ATGAAATCAT TACTCCTTTC 
TTTGCTGGTG CAACAAACTG TACAAAAGAC TTCATGGCAG TTACTTTCCC AAGACTCATT 
CCAAGCTTCA GTGATGAGCA TATGGTTCCA GCAACTCCAA TGGCCTGGAC TCTGTCAGTT 
GATGATGGAA CCAGAATACA TCAGCTGACC CTTGGACAAG CCATGCAGCA AGGCTACAAC 
TTCCTAGCTG ATGACCGCAA CCTGATCTTT CAAGTGGCCT TTGCTGCCAC TGGAGTTGTT 
TCCTACAAGC AAAATGATCA AGTGCTCTAC ACTGTGGCAC TGAAGCTCAT GTATGGCCCT 
CCTGAACACA GGCTGACTGT GGAGTCAAGA ATGCTTTGTG CACCAGGTCC AGCGATCTGT 
AATGCAACAC ACATGACTGT TGCCATCCCA GCCTTCCCAG GGATCCTTAA GGCTGTGGGT 
GTAGAGAATA AGACCATCCC CATGGACCAG CTTCAGGAAA ATGGGATTGC TCTGGACACA 
AAGAGAGGGG TCAACCTGCA TATTAGCAGG GGAGTCTTGA AGTCCAGGCT ACATGGGGAG 
AGCTGCTCAG GACTTCAGTT TTACATGTCC TCTTTGAAAC TGTCTTTTCA CTTCCATGGG 
GAGACTGTGA CAATGATGAT GCACCCAGAG TGCCCCTGTG ACCAGCACGT ACCAATAGCT 
GCTGTGTGCA CCCAGGATGG GTACATGGAT TTTGAAATCC TTGCTGACAG TACTACACCA 
CCACTAGACT TGGATGCACT TAGGCTCAGA GATCCTGCAT GCCGGCCAGT CTTTAAGTCT 
TCTTTGAATG ACAGGGTTTG GTTTCATGTC CCACTGAATG GATGTGGGAC CAGGTATTGG 
TTTGATGGGG AGAAGATTGT TTATGAGAAT GAGGTGAGGG CATTATGGGC AGACTTTCCA 
CTGCAGAGGA TCTCAAGGGA CAGTGAACTC AGACTAACAG TCCTGTGCTC CTTCAGCAAT 
GGTGATGCCT CTCTCACTAT AAAGGTGGAC AACCTTCCTC CTCTGGCTTC TTCAATGAAC 
CAAGGTCCAC TCTCCTTAGT TCTTCTAAGC TACCCAGAGG GCTCGTATAG GCAACCATAC 
CGTGATGATC AGTACCCAAT AGTAAGGTAC CTACGGCAGC CCATCTTCCT GGAAGTTCAG 
GTCCTGAATC GCAATGATCC CAACCTCCAC CTGGTATTGG ATGACTGTTG GGCAACAGCT 
TCACAAGATC CAAGTTCACT TCCCCAGTGG AATATTGTTG TTGATGGGTG TGAGTACAGC 
CTGGACAGCT ACAGGACTGT GTTTCATCCT GTGGGGCATG GTGTCAGCTA CGCTAACTAT 
CGCCAAAGGC TGGAAGTGAA GACTTTTGCC TTTGTCTCTG GTGACAAAGC CCTTTCTGGC 
CTAGTATATT TCCATTGCAG TGTTCTGATT TGTGACCGTT TTCAACCAGA CTCCCCTTTG 
TGTGTGACAA GATGCCCTAG GCCATCTAGA AGCAAGCGAG AGAGCGGGAT GCCAGGTGTG 
AACTCTGCAG TGGTGAGCTT GCGGGGTCCT GTCCTCCTTG TGCCAGAAAG ATGGTCTGCA 
ACCCAAGGGA GCACTCCCTT GAACAAGGTG TGGGCTGGCA TTACAATGAC TGCTGCTGGT 
GTTTTCTCTC TGGTTGCCAC AATGCTATTA TTTTTTGCTG CTCTTAAATG TCTAAAGAGA 
AGAGCATCCA CGGTAAATGT GGTATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014791683.1
CDS	1..2130
Translation	MGLFVEQQFLCTSRMWLLLLFGLLLPLAPCADGLGDQDLLESVTCHRDRMEVEFYGELGNYSWHVCVVDVSGEEMVSCNYTVDYERLLLSVLFVNCTSLEHDQHLLRLRLMVNDTMGEEKNVTYSTQCNTVHADEIITPFFAGATNCTKDFMAVTFPRLIPSFSDEHMVPATPMAWTLSVDDGTRIHQLTLGQAMQQGYNFLADDRNLIFQVAFAATGVVSYKQNDQVLYTVALKLMYGPPEHRLTVESRMLCAPGPAICNATHMTVAIPAFPGILKAVGVENKTIPMDQLQENGIALDTKRGVNLHISRGVLKSRLHGESCSGLQFYMSSLKLSFHFHGETVTMMMHPECPCDQHVPIAAVCTQDGYMDFEILADSTTPPLDLDALRLRDPACRPVFKSSLNDRVWFHVPLNGCGTRYWFDGEKIVYENEVRALWADFPLQRISRDSELRLTVLCSFSNGDASLTIKVDNLPPLASSMNQGPLSLVLLSYPEGSYRQPYRDDQYPIVRYLRQPIFLEVQVLNRNDPNLHLVLDDCWATASQDPSSLPQWNIVVDGCEYSLDSYRTVFHPVGHGVSYANYRQRLEVKTFAFVSGDKALSGLVYFHCSVLICDRFQPDSPLCVTRCPRPSRSKRESGMPGVNSAVVSLRGPVLLVPERWSATQGSTPLNKVWAGITMTAAGVFSLVATMLLFFAALKCLKRRASTVNVVC

Target ORF information:

RefSeq Version	XM_014936197.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax zona pellucida glycoprotein 2 (sperm receptor) (ZP2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014936197.1

ORF Insert Sequence:

ATGGGACTGT TTGTGGAGCA GCAGTTCTTA TGCACCTCAA GGATGTGGTT GCTGCTCCTG 
TTTGGCCTTT TGCTGCCTTT GGCCCCTTGT GCTGATGGCC TGGGGGATCA GGATCTCTTG 
GAGAGTGTGA CCTGTCACAG GGACAGGATG GAAGTTGAGT TTTATGGAGA GCTTGGAAAC 
TACTCCTGGC ACGTGTGTGT TGTGGATGTG AGTGGTGAGG AGATGGTGTC CTGCAACTAC 
ACTGTGGATT ATGAGAGGCT GTTGCTCAGT GTCCTGTTTG TGAACTGCAC AAGCCTGGAG 
CATGATCAGC ACCTGCTAAG ATTGAGGCTG ATGGTAAATG ACACAATGGG AGAGGAGAAG 
AATGTAACCT ACAGCACTCA GTGCAATACT GTTCATGCAG ATGAAATCAT TACTCCTTTC 
TTTGCTGGTG CAACAAACTG TACAAAAGAC TTCATGGCAG TTACTTTCCC AAGACTCATT 
CCAAGCTTCA GTGATGAGCA TATGGTTCCA GCAACTCCAA TGGCCTGGAC TCTGTCAGTT 
GATGATGGAA CCAGAATACA TCAGCTGACC CTTGGACAAG CCATGCAGCA AGGCTACAAC 
TTCCTAGCTG ATGACCGCAA CCTGATCTTT CAAGTGGCCT TTGCTGCCAC TGGAGTTGTT 
TCCTACAAGC AAAATGATCA AGTGCTCTAC ACTGTGGCAC TGAAGCTCAT GTATGGCCCT 
CCTGAACACA GGCTGACTGT GGAGTCAAGA ATGCTTTGTG CACCAGGTCC AGCGATCTGT 
AATGCAACAC ACATGACTGT TGCCATCCCA GCCTTCCCAG GGATCCTTAA GGCTGTGGGT 
GTAGAGAATA AGACCATCCC CATGGACCAG CTTCAGGAAA ATGGGATTGC TCTGGACACA 
AAGAGAGGGG TCAACCTGCA TATTAGCAGG GGAGTCTTGA AGTCCAGGCT ACATGGGGAG 
AGCTGCTCAG GACTTCAGTT TTACATGTCC TCTTTGAAAC TGTCTTTTCA CTTCCATGGG 
GAGACTGTGA CAATGATGAT GCACCCAGAG TGCCCCTGTG ACCAGCACGT ACCAATAGCT 
GCTGTGTGCA CCCAGGATGG GTACATGGAT TTTGAAATCC TTGCTGACAG TACTACACCA 
CCACTAGACT TGGATGCACT TAGGCTCAGA GATCCTGCAT GCCGGCCAGT CTTTAAGTCT 
TCTTTGAATG ACAGGGTTTG GTTTCATGTC CCACTGAATG GATGTGGGAC CAGGTATTGG 
TTTGATGGGG AGAAGATTGT TTATGAGAAT GAGGTGAGGG CATTATGGGC AGACTTTCCA 
CTGCAGAGGA TCTCAAGGGA CAGTGAACTC AGACTAACAG TCCTGTGCTC CTTCAGCAAT 
GGTGATGCCT CTCTCACTAT AAAGGTGGAC AACCTTCCTC CTCTGGCTTC TTCAATGAAC 
CAAGGTCCAC TCTCCTTAGT TCTTCTAAGC TACCCAGAGG GCTCGTATAG GCAACCATAC 
CGTGATGATC AGTACCCAAT AGTAAGGTAC CTACGGCAGC CCATCTTCCT GGAAGTTCAG 
GTCCTGAATC GCAATGATCC CAACCTCCAC CTGGTATTGG ATGACTGTTG GGCAACAGCT 
TCACAAGATC CAAGTTCACT TCCCCAGTGG AATATTGTTG TTGATGGGTG TGAGTACAGC 
CTGGACAGCT ACAGGACTGT GTTTCATCCT GTGGGGCATG GTGTCAGCTA CGCTAACTAT 
CGCCAAAGGC TGGAAGTGAA GACTTTTGCC TTTGTCTCTG GTGACAAAGC CCTTTCTGGC 
CTAGTATATT TCCATTGCAG TGTTCTGATT TGTGACCGTT TTCAACCAGA CTCCCCTTTG 
TGTGTGACAA GATGCCCTAG GCCATCTAGA AGCAAGCGAG AGAGCGGGAT GCCAGGTGTG 
AACTCTGCAG TGGTGAGCTT GCGGGGTCCT GTCCTCCTTG TGCCAGAAAG ATGGTCTGCA 
ACCCAAGGGA GCACTCCCTT GAACAAGGTG TGGGCTGGCA TTACAATGAC TGCTGCTGGT 
GTTTTCTCTC TGGTTGCCAC AATGCTATTA TTTTTTGCTG CTCTTAAATG TCTAAAGAGA 
AGAGCATCCA CGGTAAATGT GGTATGCTAG