sad-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	sad-1
Entrez Gene ID	181471
Full Name	Serine/threonine kinase SAD-1
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001083292.3	NP_001076761.1	Serine/threonine kinase SAD-1
NM_001083292.1	NP_001076761.1	Serine/threonine kinase SAD-1
NM_001083291.3	NP_001076760.1	Serine/threonine kinase SAD-1
NM_001083291.2	NP_001076760.1	Serine/threonine kinase SAD-1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	BRSK2	XP_005631383.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012244	XP_320298.4
Homo sapiens (human)	BRSK2	NP_001243556.1
	BRSK2	XP_002799482.1
Caenorhabditis elegans (roundworm)	sad-1	NP_001076761.1
Mus musculus (house mouse)	Brsk2	NP_001009930.1
Rattus norvegicus (Norway rat)	Brsk2	XP_006230671.1
Gallus gallus (chicken)	BRSK2	NP_001186525.1
Danio rerio (zebrafish)	LOC100334759	XP_002666904.2
Drosophila melanogaster (fruit fly)	sff	NP_001261901.1
Danio rerio (zebrafish)	LOC100149571	XP_005166543.1
Bos taurus (cattle)	BRSK2	XP_002699458.3
Xenopus tropicalis (tropical clawed frog)	brsk2	XP_004913517.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data reveal a new and unprecedented role for 2 synapse assembly proteins, SAD-1 and SYD-2, in mediating UNC-16's organelle transport regulatory function.
Title: UNC-16 (JIP3) Acts Through Synapse-Assembly Proteins to Inhibit the Active Transport of Cell Soma Organelles to Caenorhabditis elegans Motor Neuron Axons.

crystals belonged to the monoclinic space group C2, with unit-cell parameters a = 205.4, b = 57.1, c = 71.7 A, beta = 106.1 degrees
Title: Preliminary crystallographic analysis of the kinase domain of SAD-1, a protein essential for presynaptic differentiation in Caenorhabditis elegans.

STRD-1 establishes neuronal polarity and organizes synaptic proteins in a complex with the SAD-1 kinase
Title: C. elegans STRADalpha and SAD cooperatively regulate neuronal polarity and synaptic organization.

neuronal polarity and synaptic organization have distinct temporal requirements for SAD-1
Title: A chemical-genetic strategy reveals distinct temporal requirements for SAD-1 kinase in neuronal polarization and synapse formation.

Neurabin functions as a scaffold to facilitate SAD-1-mediated phosphorylation for substrates specific for restricting axonal fate during neuronal polarization.
Title: Neuronal polarity is regulated by a direct interaction between a scaffolding protein, Neurabin, and a presynaptic SAD-1 kinase in Caenorhabditis elegans.

The following sad-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sad-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa348633	NM_001083291.3 Latest version!	Caenorhabditis elegans Serine/threonine kinase SAD-1 (sad-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OCa348938	NM_001083292.3 Latest version!	Caenorhabditis elegans Serine/threonine kinase SAD-1 (sad-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OCa348938	NM_001083292.1	Caenorhabditis elegans Serine/threonine kinase SAD-1 (sad-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OCa348633	NM_001083291.2	Caenorhabditis elegans Serine/threonine kinase SAD-1 (sad-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sad-1 ( NM_001083291.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001076760.1 Caenorhabditis elegans Serine/threonine kinase SAD-1 (sad-1), mRNA.

Price & Availability↑

CloneID	OCa348633
Clone ID Related Accession (Same CDS sequence)	NM_001083291.3 , NM_001083291.2
Accession Version	NM_001083291.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Serine/threonine kinase SAD-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Sep 4, 2019 this sequence version replaced NM_001083291.2.

ATGTTTGAAG CTCTCAAGGA AGTCCTCGGA GAGATAAATA GCAAACTGGC GGCCGTGAAT 
AATGAGCTGA GCTCGAAAAT CATGTCGGAA AACATTGTAT CGACGCGTCC AGTGGCGCAG 
GCTCAATATT GCGGTCCATA CAAACTTGAG AAGACGCTTG GAAAAGGTCA AACTGGGTTG 
GTAAAAACGG GAACTCATTG CATTACGGGC CGTAAAGTGG CAATCAAGAT AGTCAACAAA 
GAAAAACTCA GTGAATCTGT TCTACAAAAG GTCGAAAGAG AAATTGCAAT TATGAAGTTG 
ATAGAGCATC CACATGTGCT TCATTTGTAC GATGTCTACG AAAATAAGAA ATATTTATAT 
TTGCTACTAG AACATGTTAG CGGTGGCGAG CTGTTCGACT ACCTTGTGCG GAAAGGACGG 
CTGATGTCGA AAGAAGCGCG AAAGTTTTTT CGTCAAATCA TCTCTGCGCT CGATTTTTGC 
CATGCTCATA ACATTTGTCA TCGAGATTTA AAACCGGAAA ACTTATTACT AGACGAGAGG 
AATAACATAA AAGTAGCAGA TTTTGGAATG GCTAGTCTGC AAGTGGAAGG GTCGATGTTG 
GAGACGAGTT GTGGATCTCC ACATTATGCA TGTCCAGAAG TTATTCGGGG TGAAAAGTAC 
GACGGTCGAA AAGCAGACGT TTGGAGTTGT GGCGTTATCC TCTACGCATT ATTAGTTGGT 
GCGCTTCCAT TTGATGATGA CAATCTCCGA AATCTTCTCG AAAAAGTGAA ACGTGGAGTT 
TTTCATATCC CACATTTCGT ACCTGCTGAT GTGCAGAGTT TACTGCGGGC AATGATAGAA 
GTAGACCCGG GGAAACGATA TTCCCTAGCC GACGTGTTCA AACATCCATG GGTATCCGGA 
ACAACAAAGG CAGATCCTGA ATTGGAGCTT CCAATGTCAC AAGTTGTGCA GACGCACGTA 
ATACCTGGTG AAGATTCGAT TGATCCGGAT GTCTTGAGGC ATATGAACTG CTTAGGATGT 
TTCAAAGACA AACAGAAGTT GATTAATGAA TTATTGTCGC CAAAACACAA CACTGAAAAG 
ATGGTATACT TTCTCCTTCT TGATCGAAAA CGACGGCGCC CTGCACAGGA AGACGACACA 
GAAATCGTTC TGCGCGGTGC CGCCCAGAAC AACGATCCGC CTAAAAAACG GACGGATTCT 
TCGAGAACAT CAAGATATCC CATGGGAAGC ATTGCTGATG GAAGTCCGAT CAATCCACGA 
AAAACGTATG GAAGGAATCA AAAGTCTGGA CGGCATTCAA GTTTGGGAGG TTCTCCTACA 
GAGTCACCGA GAAGTTCAAC TAGAGATCTG TTCGGTTCAT CGTCTAGCGG ATCTTACTCT 
GCAAGGGCTG GAGAAGATCG GGATAGAGGG CGATCTGCTT CAAGGTCCAC CAACAGCTAT 
CATTACTACA CTCAACCAGT CGATCCACAG ACTCTTGCGG AAGCAGCACG TCACGTACGT 
GACGCCCAGG AACGGAGGGA GAGCAGAGAC AGCGGTAGAG GGTCGTCTAG AAAAGAAAGC 
AAGGACAGAA GTGACAAAAG TGCCAGTAGT AGTAGTTGCA AAAATGATGC CTCGTCAACG 
TCTTCGGTGC CCCATAAGTA CAGCCCACCA TCAGTCATGT CGGAGAGTGT GGTAGTGTCT 
AGTAGTACGA TGAATTCTAC GAACTCGTCA ACAAATAGTT TGATAGCTGG TAACAGTCAG 
ACAAGCATTG GGTCGACTAG CGGTCCATGG CGATCAAAAC TCAACAACAT CAAAAACTCA 
TTCTTGGGAA CTCCGAGATT CCATCGGCGG AAAATGTCAA ATGGGACGGC AGAAAGTGAC 
AGTGAAGACA GCCAGATGAT TGACACAACT GACCTTGTCA AAAAGTCCTG GTTCGGATCT 
CTTGCTTCCA GTATGAGCGT CGAACGCGAT GACACCCACT GTGTGCCTGT TCAAGGGAAA 
ACATTGAACA GCATCAAAGC GGAGCTCATA CGGGCATTCC TTCAAATTCA TGAACTTAGT 
CACTCAGTAG TTGGTCAAAA TTGCTTTAGA GTAGAATATA AACGAGGTCC GACAGTTGGT 
GGAAGCGTTT TCAGCCGTGG AATCAAAATG AATGTTGATA TTATCCCATC TCCTCAACAA 
GTAGTGATAG CTGGAGAAAC TCCGACATAT GTAGTACAGT TTGTTCTGCT GGCGGGACCA 
GTTCGGAGGT TTAAACGACT TGTGGAACAC TTGTCAGCAA TTCTGCAAAA TTCTACTCAA 
CAAAGAGCTG ATCGACAACA ACAAGCAGCC CTAATGGTTC GACCAAGGAG GTTAAGTGAC 
TCCAGCGTTG GTAGTGCTTG TTCGGACAGT GAAAGCAATG CGAGCAGTAT TAATATGATA 
GCGCGGCATT CGGATAAAAC TGAAACAACT TCTGCCACGT CATCTGACCC TTACGGTCCA 
TCCCCGTCAA TGCGATCTGT TGGAAGTGGA ACAGCTAACT CATACAAATC GCCAACCCCT 
CATCGCCGTA ACACGACTGC TGTCACTGCT TCATCGTCAT CCGCATCAAA CCGATATGGT 
CCAAGCAGTA GCTCGTCTGG TAGTTACAGT AACAATGCTG ACTATTCCTA TCATCCCGAG 
TATTCACAGA GAAGCAATGG CTCAAGTGCT CCGAAGAATC AATACTCGCC CGGCTCACAA 
CGCAGCTTTG CGTTTTCCAT GTTCAACAAG GCTGATAAAG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001076760.1
CDS	111..2855
Translation	MFEALKEVLGEINSKLAAVNNELSSKIMSENIVSTRPVAQAQYCGPYKLEKTLGKGQTGLVKTGTHCITGRKVAIKIVNKEKLSESVLQKVEREIAIMKLIEHPHVLHLYDVYENKKYLYLLLEHVSGGELFDYLVRKGRLMSKEARKFFRQIISALDFCHAHNICHRDLKPENLLLDERNNIKVADFGMASLQVEGSMLETSCGSPHYACPEVIRGEKYDGRKADVWSCGVILYALLVGALPFDDDNLRNLLEKVKRGVFHIPHFVPADVQSLLRAMIEVDPGKRYSLADVFKHPWVSGTTKADPELELPMSQVVQTHVIPGEDSIDPDVLRHMNCLGCFKDKQKLINELLSPKHNTEKMVYFLLLDRKRRRPAQEDDTEIVLRGAAQNNDPPKKRTDSSRTSRYPMGSIADGSPINPRKTYGRNQKSGRHSSLGGSPTESPRSSTRDLFGSSSSGSYSARAGEDRDRGRSASRSTNSYHYYTQPVDPQTLAEAARHVRDAQERRESRDSGRGSSRKESKDRSDKSASSSSCKNDASSTSSVPHKYSPPSVMSESVVVSSSTMNSTNSSTNSLIAGNSQTSIGSTSGPWRSKLNNIKNSFLGTPRFHRRKMSNGTAESDSEDSQMIDTTDLVKKSWFGSLASSMSVERDDTHCVPVQGKTLNSIKAELIRAFLQIHELSHSVVGQNCFRVEYKRGPTVGGSVFSRGIKMNVDIIPSPQQVVIAGETPTYVVQFVLLAGPVRRFKRLVEHLSAILQNSTQQRADRQQQAALMVRPRRLSDSSVGSACSDSESNASSINMIARHSDKTETTSATSSDPYGPSPSMRSVGSGTANSYKSPTPHRRNTTAVTASSSSASNRYGPSSSSSGSYSNNADYSYHPEYSQRSNGSSAPKNQYSPGSQRSFAFSMFNKADKV

Target ORF information:

RefSeq Version	NM_001083291.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Serine/threonine kinase SAD-1 (sad-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083291.3

ORF Insert Sequence:

ATGTTTGAAG CTCTCAAGGA AGTCCTCGGA GAGATAAATA GCAAACTGGC GGCCGTGAAT 
AATGAGCTGA GCTCGAAAAT CATGTCGGAA AACATTGTAT CGACGCGTCC AGTGGCGCAG 
GCTCAATATT GCGGTCCATA CAAACTTGAG AAGACGCTTG GAAAAGGTCA AACTGGGTTG 
GTAAAAACGG GAACTCATTG CATTACGGGC CGTAAAGTGG CAATCAAGAT AGTCAACAAA 
GAAAAACTCA GTGAATCTGT TCTACAAAAG GTCGAAAGAG AAATTGCAAT TATGAAGTTG 
ATAGAGCATC CACATGTGCT TCATTTGTAC GATGTCTACG AAAATAAGAA ATATTTATAT 
TTGCTACTAG AACATGTTAG CGGTGGCGAG CTGTTCGACT ACCTTGTGCG GAAAGGACGG 
CTGATGTCGA AAGAAGCGCG AAAGTTTTTT CGTCAAATCA TCTCTGCGCT CGATTTTTGC 
CATGCTCATA ACATTTGTCA TCGAGATTTA AAACCGGAAA ACTTATTACT AGACGAGAGG 
AATAACATAA AAGTAGCAGA TTTTGGAATG GCTAGTCTGC AAGTGGAAGG GTCGATGTTG 
GAGACGAGTT GTGGATCTCC ACATTATGCA TGTCCAGAAG TTATTCGGGG TGAAAAGTAC 
GACGGTCGAA AAGCAGACGT TTGGAGTTGT GGCGTTATCC TCTACGCATT ATTAGTTGGT 
GCGCTTCCAT TTGATGATGA CAATCTCCGA AATCTTCTCG AAAAAGTGAA ACGTGGAGTT 
TTTCATATCC CACATTTCGT ACCTGCTGAT GTGCAGAGTT TACTGCGGGC AATGATAGAA 
GTAGACCCGG GGAAACGATA TTCCCTAGCC GACGTGTTCA AACATCCATG GGTATCCGGA 
ACAACAAAGG CAGATCCTGA ATTGGAGCTT CCAATGTCAC AAGTTGTGCA GACGCACGTA 
ATACCTGGTG AAGATTCGAT TGATCCGGAT GTCTTGAGGC ATATGAACTG CTTAGGATGT 
TTCAAAGACA AACAGAAGTT GATTAATGAA TTATTGTCGC CAAAACACAA CACTGAAAAG 
ATGGTATACT TTCTCCTTCT TGATCGAAAA CGACGGCGCC CTGCACAGGA AGACGACACA 
GAAATCGTTC TGCGCGGTGC CGCCCAGAAC AACGATCCGC CTAAAAAACG GACGGATTCT 
TCGAGAACAT CAAGATATCC CATGGGAAGC ATTGCTGATG GAAGTCCGAT CAATCCACGA 
AAAACGTATG GAAGGAATCA AAAGTCTGGA CGGCATTCAA GTTTGGGAGG TTCTCCTACA 
GAGTCACCGA GAAGTTCAAC TAGAGATCTG TTCGGTTCAT CGTCTAGCGG ATCTTACTCT 
GCAAGGGCTG GAGAAGATCG GGATAGAGGG CGATCTGCTT CAAGGTCCAC CAACAGCTAT 
CATTACTACA CTCAACCAGT CGATCCACAG ACTCTTGCGG AAGCAGCACG TCACGTACGT 
GACGCCCAGG AACGGAGGGA GAGCAGAGAC AGCGGTAGAG GGTCGTCTAG AAAAGAAAGC 
AAGGACAGAA GTGACAAAAG TGCCAGTAGT AGTAGTTGCA AAAATGATGC CTCGTCAACG 
TCTTCGGTGC CCCATAAGTA CAGCCCACCA TCAGTCATGT CGGAGAGTGT GGTAGTGTCT 
AGTAGTACGA TGAATTCTAC GAACTCGTCA ACAAATAGTT TGATAGCTGG TAACAGTCAG 
ACAAGCATTG GGTCGACTAG CGGTCCATGG CGATCAAAAC TCAACAACAT CAAAAACTCA 
TTCTTGGGAA CTCCGAGATT CCATCGGCGG AAAATGTCAA ATGGGACGGC AGAAAGTGAC 
AGTGAAGACA GCCAGATGAT TGACACAACT GACCTTGTCA AAAAGTCCTG GTTCGGATCT 
CTTGCTTCCA GTATGAGCGT CGAACGCGAT GACACCCACT GTGTGCCTGT TCAAGGGAAA 
ACATTGAACA GCATCAAAGC GGAGCTCATA CGGGCATTCC TTCAAATTCA TGAACTTAGT 
CACTCAGTAG TTGGTCAAAA TTGCTTTAGA GTAGAATATA AACGAGGTCC GACAGTTGGT 
GGAAGCGTTT TCAGCCGTGG AATCAAAATG AATGTTGATA TTATCCCATC TCCTCAACAA 
GTAGTGATAG CTGGAGAAAC TCCGACATAT GTAGTACAGT TTGTTCTGCT GGCGGGACCA 
GTTCGGAGGT TTAAACGACT TGTGGAACAC TTGTCAGCAA TTCTGCAAAA TTCTACTCAA 
CAAAGAGCTG ATCGACAACA ACAAGCAGCC CTAATGGTTC GACCAAGGAG GTTAAGTGAC 
TCCAGCGTTG GTAGTGCTTG TTCGGACAGT GAAAGCAATG CGAGCAGTAT TAATATGATA 
GCGCGGCATT CGGATAAAAC TGAAACAACT TCTGCCACGT CATCTGACCC TTACGGTCCA 
TCCCCGTCAA TGCGATCTGT TGGAAGTGGA ACAGCTAACT CATACAAATC GCCAACCCCT 
CATCGCCGTA ACACGACTGC TGTCACTGCT TCATCGTCAT CCGCATCAAA CCGATATGGT 
CCAAGCAGTA GCTCGTCTGG TAGTTACAGT AACAATGCTG ACTATTCCTA TCATCCCGAG 
TATTCACAGA GAAGCAATGG CTCAAGTGCT CCGAAGAATC AATACTCGCC CGGCTCACAA 
CGCAGCTTTG CGTTTTCCAT GTTCAACAAG GCTGATAAAG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sad-1 ( NM_001083291.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001076760.1 Caenorhabditis elegans Serine/threonine kinase SAD-1 (sad-1), partial mRNA.

Price & Availability↑

CloneID	OCa348633
Clone ID Related Accession (Same CDS sequence)	NM_001083291.3 , NM_001083291.2
Accession Version	NM_001083291.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Serine/threonine kinase SAD-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Jun 26, 2012 this sequence version replaced NM_001083291.1. COMPLETENESS: incomplete on both ends.

ATGTTTGAAG CTCTCAAGGA AGTCCTCGGA GAGATAAATA GCAAACTGGC GGCCGTGAAT 
AATGAGCTGA GCTCGAAAAT CATGTCGGAA AACATTGTAT CGACGCGTCC AGTGGCGCAG 
GCTCAATATT GCGGTCCATA CAAACTTGAG AAGACGCTTG GAAAAGGTCA AACTGGGTTG 
GTAAAAACGG GAACTCATTG CATTACGGGC CGTAAAGTGG CAATCAAGAT AGTCAACAAA 
GAAAAACTCA GTGAATCTGT TCTACAAAAG GTCGAAAGAG AAATTGCAAT TATGAAGTTG 
ATAGAGCATC CACATGTGCT TCATTTGTAC GATGTCTACG AAAATAAGAA ATATTTATAT 
TTGCTACTAG AACATGTTAG CGGTGGCGAG CTGTTCGACT ACCTTGTGCG GAAAGGACGG 
CTGATGTCGA AAGAAGCGCG AAAGTTTTTT CGTCAAATCA TCTCTGCGCT CGATTTTTGC 
CATGCTCATA ACATTTGTCA TCGAGATTTA AAACCGGAAA ACTTATTACT AGACGAGAGG 
AATAACATAA AAGTAGCAGA TTTTGGAATG GCTAGTCTGC AAGTGGAAGG GTCGATGTTG 
GAGACGAGTT GTGGATCTCC ACATTATGCA TGTCCAGAAG TTATTCGGGG TGAAAAGTAC 
GACGGTCGAA AAGCAGACGT TTGGAGTTGT GGCGTTATCC TCTACGCATT ATTAGTTGGT 
GCGCTTCCAT TTGATGATGA CAATCTCCGA AATCTTCTCG AAAAAGTGAA ACGTGGAGTT 
TTTCATATCC CACATTTCGT ACCTGCTGAT GTGCAGAGTT TACTGCGGGC AATGATAGAA 
GTAGACCCGG GGAAACGATA TTCCCTAGCC GACGTGTTCA AACATCCATG GGTATCCGGA 
ACAACAAAGG CAGATCCTGA ATTGGAGCTT CCAATGTCAC AAGTTGTGCA GACGCACGTA 
ATACCTGGTG AAGATTCGAT TGATCCGGAT GTCTTGAGGC ATATGAACTG CTTAGGATGT 
TTCAAAGACA AACAGAAGTT GATTAATGAA TTATTGTCGC CAAAACACAA CACTGAAAAG 
ATGGTATACT TTCTCCTTCT TGATCGAAAA CGACGGCGCC CTGCACAGGA AGACGACACA 
GAAATCGTTC TGCGCGGTGC CGCCCAGAAC AACGATCCGC CTAAAAAACG GACGGATTCT 
TCGAGAACAT CAAGATATCC CATGGGAAGC ATTGCTGATG GAAGTCCGAT CAATCCACGA 
AAAACGTATG GAAGGAATCA AAAGTCTGGA CGGCATTCAA GTTTGGGAGG TTCTCCTACA 
GAGTCACCGA GAAGTTCAAC TAGAGATCTG TTCGGTTCAT CGTCTAGCGG ATCTTACTCT 
GCAAGGGCTG GAGAAGATCG GGATAGAGGG CGATCTGCTT CAAGGTCCAC CAACAGCTAT 
CATTACTACA CTCAACCAGT CGATCCACAG ACTCTTGCGG AAGCAGCACG TCACGTACGT 
GACGCCCAGG AACGGAGGGA GAGCAGAGAC AGCGGTAGAG GGTCGTCTAG AAAAGAAAGC 
AAGGACAGAA GTGACAAAAG TGCCAGTAGT AGTAGTTGCA AAAATGATGC CTCGTCAACG 
TCTTCGGTGC CCCATAAGTA CAGCCCACCA TCAGTCATGT CGGAGAGTGT GGTAGTGTCT 
AGTAGTACGA TGAATTCTAC GAACTCGTCA ACAAATAGTT TGATAGCTGG TAACAGTCAG 
ACAAGCATTG GGTCGACTAG CGGTCCATGG CGATCAAAAC TCAACAACAT CAAAAACTCA 
TTCTTGGGAA CTCCGAGATT CCATCGGCGG AAAATGTCAA ATGGGACGGC AGAAAGTGAC 
AGTGAAGACA GCCAGATGAT TGACACAACT GACCTTGTCA AAAAGTCCTG GTTCGGATCT 
CTTGCTTCCA GTATGAGCGT CGAACGCGAT GACACCCACT GTGTGCCTGT TCAAGGGAAA 
ACATTGAACA GCATCAAAGC GGAGCTCATA CGGGCATTCC TTCAAATTCA TGAACTTAGT 
CACTCAGTAG TTGGTCAAAA TTGCTTTAGA GTAGAATATA AACGAGGTCC GACAGTTGGT 
GGAAGCGTTT TCAGCCGTGG AATCAAAATG AATGTTGATA TTATCCCATC TCCTCAACAA 
GTAGTGATAG CTGGAGAAAC TCCGACATAT GTAGTACAGT TTGTTCTGCT GGCGGGACCA 
GTTCGGAGGT TTAAACGACT TGTGGAACAC TTGTCAGCAA TTCTGCAAAA TTCTACTCAA 
CAAAGAGCTG ATCGACAACA ACAAGCAGCC CTAATGGTTC GACCAAGGAG GTTAAGTGAC 
TCCAGCGTTG GTAGTGCTTG TTCGGACAGT GAAAGCAATG CGAGCAGTAT TAATATGATA 
GCGCGGCATT CGGATAAAAC TGAAACAACT TCTGCCACGT CATCTGACCC TTACGGTCCA 
TCCCCGTCAA TGCGATCTGT TGGAAGTGGA ACAGCTAACT CATACAAATC GCCAACCCCT 
CATCGCCGTA ACACGACTGC TGTCACTGCT TCATCGTCAT CCGCATCAAA CCGATATGGT 
CCAAGCAGTA GCTCGTCTGG TAGTTACAGT AACAATGCTG ACTATTCCTA TCATCCCGAG 
TATTCACAGA GAAGCAATGG CTCAAGTGCT CCGAAGAATC AATACTCGCC CGGCTCACAA 
CGCAGCTTTG CGTTTTCCAT GTTCAACAAG GCTGATAAAG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001076760.1
CDS	1..2745
Translation	MFEALKEVLGEINSKLAAVNNELSSKIMSENIVSTRPVAQAQYCGPYKLEKTLGKGQTGLVKTGTHCITGRKVAIKIVNKEKLSESVLQKVEREIAIMKLIEHPHVLHLYDVYENKKYLYLLLEHVSGGELFDYLVRKGRLMSKEARKFFRQIISALDFCHAHNICHRDLKPENLLLDERNNIKVADFGMASLQVEGSMLETSCGSPHYACPEVIRGEKYDGRKADVWSCGVILYALLVGALPFDDDNLRNLLEKVKRGVFHIPHFVPADVQSLLRAMIEVDPGKRYSLADVFKHPWVSGTTKADPELELPMSQVVQTHVIPGEDSIDPDVLRHMNCLGCFKDKQKLINELLSPKHNTEKMVYFLLLDRKRRRPAQEDDTEIVLRGAAQNNDPPKKRTDSSRTSRYPMGSIADGSPINPRKTYGRNQKSGRHSSLGGSPTESPRSSTRDLFGSSSSGSYSARAGEDRDRGRSASRSTNSYHYYTQPVDPQTLAEAARHVRDAQERRESRDSGRGSSRKESKDRSDKSASSSSCKNDASSTSSVPHKYSPPSVMSESVVVSSSTMNSTNSSTNSLIAGNSQTSIGSTSGPWRSKLNNIKNSFLGTPRFHRRKMSNGTAESDSEDSQMIDTTDLVKKSWFGSLASSMSVERDDTHCVPVQGKTLNSIKAELIRAFLQIHELSHSVVGQNCFRVEYKRGPTVGGSVFSRGIKMNVDIIPSPQQVVIAGETPTYVVQFVLLAGPVRRFKRLVEHLSAILQNSTQQRADRQQQAALMVRPRRLSDSSVGSACSDSESNASSINMIARHSDKTETTSATSSDPYGPSPSMRSVGSGTANSYKSPTPHRRNTTAVTASSSSASNRYGPSSSSSGSYSNNADYSYHPEYSQRSNGSSAPKNQYSPGSQRSFAFSMFNKADKV

Target ORF information:

RefSeq Version	NM_001083291.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Serine/threonine kinase SAD-1 (sad-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083291.2

ORF Insert Sequence:

ATGTTTGAAG CTCTCAAGGA AGTCCTCGGA GAGATAAATA GCAAACTGGC GGCCGTGAAT 
AATGAGCTGA GCTCGAAAAT CATGTCGGAA AACATTGTAT CGACGCGTCC AGTGGCGCAG 
GCTCAATATT GCGGTCCATA CAAACTTGAG AAGACGCTTG GAAAAGGTCA AACTGGGTTG 
GTAAAAACGG GAACTCATTG CATTACGGGC CGTAAAGTGG CAATCAAGAT AGTCAACAAA 
GAAAAACTCA GTGAATCTGT TCTACAAAAG GTCGAAAGAG AAATTGCAAT TATGAAGTTG 
ATAGAGCATC CACATGTGCT TCATTTGTAC GATGTCTACG AAAATAAGAA ATATTTATAT 
TTGCTACTAG AACATGTTAG CGGTGGCGAG CTGTTCGACT ACCTTGTGCG GAAAGGACGG 
CTGATGTCGA AAGAAGCGCG AAAGTTTTTT CGTCAAATCA TCTCTGCGCT CGATTTTTGC 
CATGCTCATA ACATTTGTCA TCGAGATTTA AAACCGGAAA ACTTATTACT AGACGAGAGG 
AATAACATAA AAGTAGCAGA TTTTGGAATG GCTAGTCTGC AAGTGGAAGG GTCGATGTTG 
GAGACGAGTT GTGGATCTCC ACATTATGCA TGTCCAGAAG TTATTCGGGG TGAAAAGTAC 
GACGGTCGAA AAGCAGACGT TTGGAGTTGT GGCGTTATCC TCTACGCATT ATTAGTTGGT 
GCGCTTCCAT TTGATGATGA CAATCTCCGA AATCTTCTCG AAAAAGTGAA ACGTGGAGTT 
TTTCATATCC CACATTTCGT ACCTGCTGAT GTGCAGAGTT TACTGCGGGC AATGATAGAA 
GTAGACCCGG GGAAACGATA TTCCCTAGCC GACGTGTTCA AACATCCATG GGTATCCGGA 
ACAACAAAGG CAGATCCTGA ATTGGAGCTT CCAATGTCAC AAGTTGTGCA GACGCACGTA 
ATACCTGGTG AAGATTCGAT TGATCCGGAT GTCTTGAGGC ATATGAACTG CTTAGGATGT 
TTCAAAGACA AACAGAAGTT GATTAATGAA TTATTGTCGC CAAAACACAA CACTGAAAAG 
ATGGTATACT TTCTCCTTCT TGATCGAAAA CGACGGCGCC CTGCACAGGA AGACGACACA 
GAAATCGTTC TGCGCGGTGC CGCCCAGAAC AACGATCCGC CTAAAAAACG GACGGATTCT 
TCGAGAACAT CAAGATATCC CATGGGAAGC ATTGCTGATG GAAGTCCGAT CAATCCACGA 
AAAACGTATG GAAGGAATCA AAAGTCTGGA CGGCATTCAA GTTTGGGAGG TTCTCCTACA 
GAGTCACCGA GAAGTTCAAC TAGAGATCTG TTCGGTTCAT CGTCTAGCGG ATCTTACTCT 
GCAAGGGCTG GAGAAGATCG GGATAGAGGG CGATCTGCTT CAAGGTCCAC CAACAGCTAT 
CATTACTACA CTCAACCAGT CGATCCACAG ACTCTTGCGG AAGCAGCACG TCACGTACGT 
GACGCCCAGG AACGGAGGGA GAGCAGAGAC AGCGGTAGAG GGTCGTCTAG AAAAGAAAGC 
AAGGACAGAA GTGACAAAAG TGCCAGTAGT AGTAGTTGCA AAAATGATGC CTCGTCAACG 
TCTTCGGTGC CCCATAAGTA CAGCCCACCA TCAGTCATGT CGGAGAGTGT GGTAGTGTCT 
AGTAGTACGA TGAATTCTAC GAACTCGTCA ACAAATAGTT TGATAGCTGG TAACAGTCAG 
ACAAGCATTG GGTCGACTAG CGGTCCATGG CGATCAAAAC TCAACAACAT CAAAAACTCA 
TTCTTGGGAA CTCCGAGATT CCATCGGCGG AAAATGTCAA ATGGGACGGC AGAAAGTGAC 
AGTGAAGACA GCCAGATGAT TGACACAACT GACCTTGTCA AAAAGTCCTG GTTCGGATCT 
CTTGCTTCCA GTATGAGCGT CGAACGCGAT GACACCCACT GTGTGCCTGT TCAAGGGAAA 
ACATTGAACA GCATCAAAGC GGAGCTCATA CGGGCATTCC TTCAAATTCA TGAACTTAGT 
CACTCAGTAG TTGGTCAAAA TTGCTTTAGA GTAGAATATA AACGAGGTCC GACAGTTGGT 
GGAAGCGTTT TCAGCCGTGG AATCAAAATG AATGTTGATA TTATCCCATC TCCTCAACAA 
GTAGTGATAG CTGGAGAAAC TCCGACATAT GTAGTACAGT TTGTTCTGCT GGCGGGACCA 
GTTCGGAGGT TTAAACGACT TGTGGAACAC TTGTCAGCAA TTCTGCAAAA TTCTACTCAA 
CAAAGAGCTG ATCGACAACA ACAAGCAGCC CTAATGGTTC GACCAAGGAG GTTAAGTGAC 
TCCAGCGTTG GTAGTGCTTG TTCGGACAGT GAAAGCAATG CGAGCAGTAT TAATATGATA 
GCGCGGCATT CGGATAAAAC TGAAACAACT TCTGCCACGT CATCTGACCC TTACGGTCCA 
TCCCCGTCAA TGCGATCTGT TGGAAGTGGA ACAGCTAACT CATACAAATC GCCAACCCCT 
CATCGCCGTA ACACGACTGC TGTCACTGCT TCATCGTCAT CCGCATCAAA CCGATATGGT 
CCAAGCAGTA GCTCGTCTGG TAGTTACAGT AACAATGCTG ACTATTCCTA TCATCCCGAG 
TATTCACAGA GAAGCAATGG CTCAAGTGCT CCGAAGAATC AATACTCGCC CGGCTCACAA 
CGCAGCTTTG CGTTTTCCAT GTTCAACAAG GCTGATAAAG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sad-1 ( NM_001083292.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001076761.1 Caenorhabditis elegans Serine/threonine kinase SAD-1 (sad-1), mRNA.

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CloneID	OCa348938
Clone ID Related Accession (Same CDS sequence)	NM_001083292.3 , NM_001083292.1
Accession Version	NM_001083292.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Serine/threonine kinase SAD-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Oct 18, 2019 this sequence version replaced NM_001083292.2.

ATGTTTGAAG CTCTCAAGGA AGTCCTCGGA GAGATAAATA GCAAACTGGC GGCCGTGAAT 
AATGAGCTGA GCTCGAAAAT CATGTCGGAA AACATTGTAT CGACGCGTCC AGTGGCGCAG 
GCTCAATATT GCGGTCCATA CAAACTTGAG AAGACGCTTG GAAAAGGTCA AACTGGGTTG 
GTAAAAACGG GAACTCATTG CATTACGGGC CGTAAAGTGG CAATCAAGAT AGTCAACAAA 
GAAAAACTCA GTGAATCTGT TCTACAAAAG GTCGAAAGAG AAATTGCAAT TATGAAGTTG 
ATAGAGCATC CACATGTGCT TCATTTGTAC GATGTCTACG AAAATAAGAA ATATTTATAT 
TTGCTACTAG AACATGTTAG CGGTGGCGAG CTGTTCGACT ACCTTGTGCG GAAAGGACGG 
CTGATGTCGA AAGAAGCGCG AAAGTTTTTT CGTCAAATCA TCTCTGCGCT CGATTTTTGC 
CATGCTCATA ACATTTGTCA TCGAGATTTA AAACCGGAAA ACTTATTACT AGACGAGAGG 
AATAACATAA AAGTAGCAGA TTTTGGAATG GCTAGTCTGC AAGTGGAAGG GTCGATGTTG 
GAGACGAGTT GTGGATCTCC ACATTATGCA TGTCCAGAAG TTATTCGGGG TGAAAAGTAC 
GACGGTCGAA AAGCAGACGT TTGGAGTTGT GGCGTTATCC TCTACGCATT ATTAGTTGGT 
GCGCTTCCAT TTGATGATGA CAATCTCCGA AATCTTCTCG AAAAAGTGAA ACGTGGAGTT 
TTTCATATCC CACATTTCGT ACCTGCTGAT GTGCAGAGTT TACTGCGGGC AATGATAGAA 
GTAGACCCGG GGAAACGATA TTCCCTAGCC GACGTGTTCA AACATCCATG GGTATCCGGA 
ACAACAAAGG CAGATCCTGA ATTGGAGCTT CCAATGTCAC AAGTTGTGCA GACGCACGTA 
ATACCTGGTG AAGATTCGAT TGATCCGGAT GTCTTGAGGC ATATGAACTG CTTAGGATGT 
TTCAAAGACA AACAGAAGTT GATTAATGAA TTATTGTCGC CAAAACACAA CACTGAAAAG 
ATGGTATACT TTCTCCTTCT TGATCGAAAA CGACGGCGCC CTGCACAGGA AGACGACACA 
GAAATCGTTC TGCGCGGTGC CGCCCAGAAC AACGATCCGC CTAAAAAACG GACGGATTCT 
TCGAGAACAT CAAGATATCC CATGGGAAGC ATTGCTGATG GAAGTCCGAT CAATCCACGA 
AAAACGTATG GAAGGAATCA AAAGTCTGGA CGGCATTCAA GTTTGGGAGG TTCTCCTACA 
GAGTCACCGA GAAGTTCAAC TAGAGATCTG TTCGGTTCAT CGTCTAGCGG ATCTTACTCT 
GCAAGGGCTG GAGAAGATCG GGATAGAGGG CGATCTGCTT CAAGGTCCAC CAACAGCTAT 
CATTACTACA CTCAACCAGT CGATCCACAG ACTCTTGCGG AAGCAGCACG TCACGTACGT 
GACGCCCAGG AACGGAGGGA GAGCAGAGAC AGCGGTAGAG GGTCGTCTAG AAAAGAAAGC 
AAGGACAGAA GTGACAAAAG TGCCAGTAGT AGTAGTTGCA AAAATGATGC CTCGTCAACG 
TCTTCGGTGC CCCATAAGTA CAGCCCACCA TCAGTCATGT CGGAGAGTGT GGTAGTGTCT 
AGTAGTACGA TGAATTCTAC GAACTCGTCA ACAAATAGTT TGATAGCTGG TAACAGTCAG 
ACAAGCATTG GGTCGACTAG CGGTCCATGG CGATCAAAAC TCAACAACAT CAAAAACTCA 
TTCTTGGGAA CTCCGAGATT CCATCGGCGG AAAATGTCAA ATGGGACGGC AGAAAGTGAC 
AGTGAAGACA GCCAGATGAT TGACACAACT GACCTTGTCA AAAAGTCCTG GTTCGGATCT 
CTTGCTTCCA GTATGAGCGT CGAACGCGAT GACACCCACT GTGTGCCTGT TCAAGGGAAA 
ACATTGAACA GCATCAAAGC GGAGCTCATA CGGGCATTCC TTCAAATTCA TGAACTTAGT 
CACTCAGTAG TTGGTCAAAA TTGCTTTAGA GTAGAATATA AACGAGGTCC GACAGTTGGT 
GGAAGCGTTT TCAGCCGTGG AATCAAAATG AATGTTGATA TTATCCCATC TCCTCAACAA 
GTAGTGATAG CTGGAGAAAC TCCGACATAT GTAGTACAGT TTGTTCTGCT GGCGGGACCA 
GTTCGGAGGT TTAAACGACT TGTGGAACAC TTGTCAGCAA TTCTGCAAAA TTCTACTCAA 
CAAAGAGCTG ATCGACAACA ACAAGCAGCC CTAATGGTTC GACCAAGGAG GTTAAGTGAC 
TCCAGCGTTG GTAGTGCTTG TTCGGACAGT GAAAGCAATG CGAGCAGTAT TAATATGATA 
GCGCGGCATT CGGATAAAAC TGAAACAACT TCTGCCACGT CATCTGACCC TTACGGTCCA 
TCCCCGTCAA TGCGATCTGT TGGAAGTGGA ACAGGAATTA CATCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001076761.1
CDS	111..2618
Translation	MFEALKEVLGEINSKLAAVNNELSSKIMSENIVSTRPVAQAQYCGPYKLEKTLGKGQTGLVKTGTHCITGRKVAIKIVNKEKLSESVLQKVEREIAIMKLIEHPHVLHLYDVYENKKYLYLLLEHVSGGELFDYLVRKGRLMSKEARKFFRQIISALDFCHAHNICHRDLKPENLLLDERNNIKVADFGMASLQVEGSMLETSCGSPHYACPEVIRGEKYDGRKADVWSCGVILYALLVGALPFDDDNLRNLLEKVKRGVFHIPHFVPADVQSLLRAMIEVDPGKRYSLADVFKHPWVSGTTKADPELELPMSQVVQTHVIPGEDSIDPDVLRHMNCLGCFKDKQKLINELLSPKHNTEKMVYFLLLDRKRRRPAQEDDTEIVLRGAAQNNDPPKKRTDSSRTSRYPMGSIADGSPINPRKTYGRNQKSGRHSSLGGSPTESPRSSTRDLFGSSSSGSYSARAGEDRDRGRSASRSTNSYHYYTQPVDPQTLAEAARHVRDAQERRESRDSGRGSSRKESKDRSDKSASSSSCKNDASSTSSVPHKYSPPSVMSESVVVSSSTMNSTNSSTNSLIAGNSQTSIGSTSGPWRSKLNNIKNSFLGTPRFHRRKMSNGTAESDSEDSQMIDTTDLVKKSWFGSLASSMSVERDDTHCVPVQGKTLNSIKAELIRAFLQIHELSHSVVGQNCFRVEYKRGPTVGGSVFSRGIKMNVDIIPSPQQVVIAGETPTYVVQFVLLAGPVRRFKRLVEHLSAILQNSTQQRADRQQQAALMVRPRRLSDSSVGSACSDSESNASSINMIARHSDKTETTSATSSDPYGPSPSMRSVGSGTGITS

Target ORF information:

RefSeq Version	NM_001083292.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Serine/threonine kinase SAD-1 (sad-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083292.3

ORF Insert Sequence:

ATGTTTGAAG CTCTCAAGGA AGTCCTCGGA GAGATAAATA GCAAACTGGC GGCCGTGAAT 
AATGAGCTGA GCTCGAAAAT CATGTCGGAA AACATTGTAT CGACGCGTCC AGTGGCGCAG 
GCTCAATATT GCGGTCCATA CAAACTTGAG AAGACGCTTG GAAAAGGTCA AACTGGGTTG 
GTAAAAACGG GAACTCATTG CATTACGGGC CGTAAAGTGG CAATCAAGAT AGTCAACAAA 
GAAAAACTCA GTGAATCTGT TCTACAAAAG GTCGAAAGAG AAATTGCAAT TATGAAGTTG 
ATAGAGCATC CACATGTGCT TCATTTGTAC GATGTCTACG AAAATAAGAA ATATTTATAT 
TTGCTACTAG AACATGTTAG CGGTGGCGAG CTGTTCGACT ACCTTGTGCG GAAAGGACGG 
CTGATGTCGA AAGAAGCGCG AAAGTTTTTT CGTCAAATCA TCTCTGCGCT CGATTTTTGC 
CATGCTCATA ACATTTGTCA TCGAGATTTA AAACCGGAAA ACTTATTACT AGACGAGAGG 
AATAACATAA AAGTAGCAGA TTTTGGAATG GCTAGTCTGC AAGTGGAAGG GTCGATGTTG 
GAGACGAGTT GTGGATCTCC ACATTATGCA TGTCCAGAAG TTATTCGGGG TGAAAAGTAC 
GACGGTCGAA AAGCAGACGT TTGGAGTTGT GGCGTTATCC TCTACGCATT ATTAGTTGGT 
GCGCTTCCAT TTGATGATGA CAATCTCCGA AATCTTCTCG AAAAAGTGAA ACGTGGAGTT 
TTTCATATCC CACATTTCGT ACCTGCTGAT GTGCAGAGTT TACTGCGGGC AATGATAGAA 
GTAGACCCGG GGAAACGATA TTCCCTAGCC GACGTGTTCA AACATCCATG GGTATCCGGA 
ACAACAAAGG CAGATCCTGA ATTGGAGCTT CCAATGTCAC AAGTTGTGCA GACGCACGTA 
ATACCTGGTG AAGATTCGAT TGATCCGGAT GTCTTGAGGC ATATGAACTG CTTAGGATGT 
TTCAAAGACA AACAGAAGTT GATTAATGAA TTATTGTCGC CAAAACACAA CACTGAAAAG 
ATGGTATACT TTCTCCTTCT TGATCGAAAA CGACGGCGCC CTGCACAGGA AGACGACACA 
GAAATCGTTC TGCGCGGTGC CGCCCAGAAC AACGATCCGC CTAAAAAACG GACGGATTCT 
TCGAGAACAT CAAGATATCC CATGGGAAGC ATTGCTGATG GAAGTCCGAT CAATCCACGA 
AAAACGTATG GAAGGAATCA AAAGTCTGGA CGGCATTCAA GTTTGGGAGG TTCTCCTACA 
GAGTCACCGA GAAGTTCAAC TAGAGATCTG TTCGGTTCAT CGTCTAGCGG ATCTTACTCT 
GCAAGGGCTG GAGAAGATCG GGATAGAGGG CGATCTGCTT CAAGGTCCAC CAACAGCTAT 
CATTACTACA CTCAACCAGT CGATCCACAG ACTCTTGCGG AAGCAGCACG TCACGTACGT 
GACGCCCAGG AACGGAGGGA GAGCAGAGAC AGCGGTAGAG GGTCGTCTAG AAAAGAAAGC 
AAGGACAGAA GTGACAAAAG TGCCAGTAGT AGTAGTTGCA AAAATGATGC CTCGTCAACG 
TCTTCGGTGC CCCATAAGTA CAGCCCACCA TCAGTCATGT CGGAGAGTGT GGTAGTGTCT 
AGTAGTACGA TGAATTCTAC GAACTCGTCA ACAAATAGTT TGATAGCTGG TAACAGTCAG 
ACAAGCATTG GGTCGACTAG CGGTCCATGG CGATCAAAAC TCAACAACAT CAAAAACTCA 
TTCTTGGGAA CTCCGAGATT CCATCGGCGG AAAATGTCAA ATGGGACGGC AGAAAGTGAC 
AGTGAAGACA GCCAGATGAT TGACACAACT GACCTTGTCA AAAAGTCCTG GTTCGGATCT 
CTTGCTTCCA GTATGAGCGT CGAACGCGAT GACACCCACT GTGTGCCTGT TCAAGGGAAA 
ACATTGAACA GCATCAAAGC GGAGCTCATA CGGGCATTCC TTCAAATTCA TGAACTTAGT 
CACTCAGTAG TTGGTCAAAA TTGCTTTAGA GTAGAATATA AACGAGGTCC GACAGTTGGT 
GGAAGCGTTT TCAGCCGTGG AATCAAAATG AATGTTGATA TTATCCCATC TCCTCAACAA 
GTAGTGATAG CTGGAGAAAC TCCGACATAT GTAGTACAGT TTGTTCTGCT GGCGGGACCA 
GTTCGGAGGT TTAAACGACT TGTGGAACAC TTGTCAGCAA TTCTGCAAAA TTCTACTCAA 
CAAAGAGCTG ATCGACAACA ACAAGCAGCC CTAATGGTTC GACCAAGGAG GTTAAGTGAC 
TCCAGCGTTG GTAGTGCTTG TTCGGACAGT GAAAGCAATG CGAGCAGTAT TAATATGATA 
GCGCGGCATT CGGATAAAAC TGAAACAACT TCTGCCACGT CATCTGACCC TTACGGTCCA 
TCCCCGTCAA TGCGATCTGT TGGAAGTGGA ACAGGAATTA CATCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sad-1 ( NM_001083292.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001076761.1 Caenorhabditis elegans Serine/threonine kinase SAD-1 (sad-1), partial mRNA.

Price & Availability↑

CloneID	OCa348938
Clone ID Related Accession (Same CDS sequence)	NM_001083292.3 , NM_001083292.1
Accession Version	NM_001083292.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Serine/threonine kinase SAD-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). COMPLETENESS: incomplete on both ends.

ATGTTTGAAG CTCTCAAGGA AGTCCTCGGA GAGATAAATA GCAAACTGGC GGCCGTGAAT 
AATGAGCTGA GCTCGAAAAT CATGTCGGAA AACATTGTAT CGACGCGTCC AGTGGCGCAG 
GCTCAATATT GCGGTCCATA CAAACTTGAG AAGACGCTTG GAAAAGGTCA AACTGGGTTG 
GTAAAAACGG GAACTCATTG CATTACGGGC CGTAAAGTGG CAATCAAGAT AGTCAACAAA 
GAAAAACTCA GTGAATCTGT TCTACAAAAG GTCGAAAGAG AAATTGCAAT TATGAAGTTG 
ATAGAGCATC CACATGTGCT TCATTTGTAC GATGTCTACG AAAATAAGAA ATATTTATAT 
TTGCTACTAG AACATGTTAG CGGTGGCGAG CTGTTCGACT ACCTTGTGCG GAAAGGACGG 
CTGATGTCGA AAGAAGCGCG AAAGTTTTTT CGTCAAATCA TCTCTGCGCT CGATTTTTGC 
CATGCTCATA ACATTTGTCA TCGAGATTTA AAACCGGAAA ACTTATTACT AGACGAGAGG 
AATAACATAA AAGTAGCAGA TTTTGGAATG GCTAGTCTGC AAGTGGAAGG GTCGATGTTG 
GAGACGAGTT GTGGATCTCC ACATTATGCA TGTCCAGAAG TTATTCGGGG TGAAAAGTAC 
GACGGTCGAA AAGCAGACGT TTGGAGTTGT GGCGTTATCC TCTACGCATT ATTAGTTGGT 
GCGCTTCCAT TTGATGATGA CAATCTCCGA AATCTTCTCG AAAAAGTGAA ACGTGGAGTT 
TTTCATATCC CACATTTCGT ACCTGCTGAT GTGCAGAGTT TACTGCGGGC AATGATAGAA 
GTAGACCCGG GGAAACGATA TTCCCTAGCC GACGTGTTCA AACATCCATG GGTATCCGGA 
ACAACAAAGG CAGATCCTGA ATTGGAGCTT CCAATGTCAC AAGTTGTGCA GACGCACGTA 
ATACCTGGTG AAGATTCGAT TGATCCGGAT GTCTTGAGGC ATATGAACTG CTTAGGATGT 
TTCAAAGACA AACAGAAGTT GATTAATGAA TTATTGTCGC CAAAACACAA CACTGAAAAG 
ATGGTATACT TTCTCCTTCT TGATCGAAAA CGACGGCGCC CTGCACAGGA AGACGACACA 
GAAATCGTTC TGCGCGGTGC CGCCCAGAAC AACGATCCGC CTAAAAAACG GACGGATTCT 
TCGAGAACAT CAAGATATCC CATGGGAAGC ATTGCTGATG GAAGTCCGAT CAATCCACGA 
AAAACGTATG GAAGGAATCA AAAGTCTGGA CGGCATTCAA GTTTGGGAGG TTCTCCTACA 
GAGTCACCGA GAAGTTCAAC TAGAGATCTG TTCGGTTCAT CGTCTAGCGG ATCTTACTCT 
GCAAGGGCTG GAGAAGATCG GGATAGAGGG CGATCTGCTT CAAGGTCCAC CAACAGCTAT 
CATTACTACA CTCAACCAGT CGATCCACAG ACTCTTGCGG AAGCAGCACG TCACGTACGT 
GACGCCCAGG AACGGAGGGA GAGCAGAGAC AGCGGTAGAG GGTCGTCTAG AAAAGAAAGC 
AAGGACAGAA GTGACAAAAG TGCCAGTAGT AGTAGTTGCA AAAATGATGC CTCGTCAACG 
TCTTCGGTGC CCCATAAGTA CAGCCCACCA TCAGTCATGT CGGAGAGTGT GGTAGTGTCT 
AGTAGTACGA TGAATTCTAC GAACTCGTCA ACAAATAGTT TGATAGCTGG TAACAGTCAG 
ACAAGCATTG GGTCGACTAG CGGTCCATGG CGATCAAAAC TCAACAACAT CAAAAACTCA 
TTCTTGGGAA CTCCGAGATT CCATCGGCGG AAAATGTCAA ATGGGACGGC AGAAAGTGAC 
AGTGAAGACA GCCAGATGAT TGACACAACT GACCTTGTCA AAAAGTCCTG GTTCGGATCT 
CTTGCTTCCA GTATGAGCGT CGAACGCGAT GACACCCACT GTGTGCCTGT TCAAGGGAAA 
ACATTGAACA GCATCAAAGC GGAGCTCATA CGGGCATTCC TTCAAATTCA TGAACTTAGT 
CACTCAGTAG TTGGTCAAAA TTGCTTTAGA GTAGAATATA AACGAGGTCC GACAGTTGGT 
GGAAGCGTTT TCAGCCGTGG AATCAAAATG AATGTTGATA TTATCCCATC TCCTCAACAA 
GTAGTGATAG CTGGAGAAAC TCCGACATAT GTAGTACAGT TTGTTCTGCT GGCGGGACCA 
GTTCGGAGGT TTAAACGACT TGTGGAACAC TTGTCAGCAA TTCTGCAAAA TTCTACTCAA 
CAAAGAGCTG ATCGACAACA ACAAGCAGCC CTAATGGTTC GACCAAGGAG GTTAAGTGAC 
TCCAGCGTTG GTAGTGCTTG TTCGGACAGT GAAAGCAATG CGAGCAGTAT TAATATGATA 
GCGCGGCATT CGGATAAAAC TGAAACAACT TCTGCCACGT CATCTGACCC TTACGGTCCA 
TCCCCGTCAA TGCGATCTGT TGGAAGTGGA ACAGGAATTA CATCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001076761.1
CDS	1..2508
Translation	MFEALKEVLGEINSKLAAVNNELSSKIMSENIVSTRPVAQAQYCGPYKLEKTLGKGQTGLVKTGTHCITGRKVAIKIVNKEKLSESVLQKVEREIAIMKLIEHPHVLHLYDVYENKKYLYLLLEHVSGGELFDYLVRKGRLMSKEARKFFRQIISALDFCHAHNICHRDLKPENLLLDERNNIKVADFGMASLQVEGSMLETSCGSPHYACPEVIRGEKYDGRKADVWSCGVILYALLVGALPFDDDNLRNLLEKVKRGVFHIPHFVPADVQSLLRAMIEVDPGKRYSLADVFKHPWVSGTTKADPELELPMSQVVQTHVIPGEDSIDPDVLRHMNCLGCFKDKQKLINELLSPKHNTEKMVYFLLLDRKRRRPAQEDDTEIVLRGAAQNNDPPKKRTDSSRTSRYPMGSIADGSPINPRKTYGRNQKSGRHSSLGGSPTESPRSSTRDLFGSSSSGSYSARAGEDRDRGRSASRSTNSYHYYTQPVDPQTLAEAARHVRDAQERRESRDSGRGSSRKESKDRSDKSASSSSCKNDASSTSSVPHKYSPPSVMSESVVVSSSTMNSTNSSTNSLIAGNSQTSIGSTSGPWRSKLNNIKNSFLGTPRFHRRKMSNGTAESDSEDSQMIDTTDLVKKSWFGSLASSMSVERDDTHCVPVQGKTLNSIKAELIRAFLQIHELSHSVVGQNCFRVEYKRGPTVGGSVFSRGIKMNVDIIPSPQQVVIAGETPTYVVQFVLLAGPVRRFKRLVEHLSAILQNSTQQRADRQQQAALMVRPRRLSDSSVGSACSDSESNASSINMIARHSDKTETTSATSSDPYGPSPSMRSVGSGTGITS

Target ORF information:

RefSeq Version	NM_001083292.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Serine/threonine kinase SAD-1 (sad-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083292.1

ORF Insert Sequence:

ATGTTTGAAG CTCTCAAGGA AGTCCTCGGA GAGATAAATA GCAAACTGGC GGCCGTGAAT 
AATGAGCTGA GCTCGAAAAT CATGTCGGAA AACATTGTAT CGACGCGTCC AGTGGCGCAG 
GCTCAATATT GCGGTCCATA CAAACTTGAG AAGACGCTTG GAAAAGGTCA AACTGGGTTG 
GTAAAAACGG GAACTCATTG CATTACGGGC CGTAAAGTGG CAATCAAGAT AGTCAACAAA 
GAAAAACTCA GTGAATCTGT TCTACAAAAG GTCGAAAGAG AAATTGCAAT TATGAAGTTG 
ATAGAGCATC CACATGTGCT TCATTTGTAC GATGTCTACG AAAATAAGAA ATATTTATAT 
TTGCTACTAG AACATGTTAG CGGTGGCGAG CTGTTCGACT ACCTTGTGCG GAAAGGACGG 
CTGATGTCGA AAGAAGCGCG AAAGTTTTTT CGTCAAATCA TCTCTGCGCT CGATTTTTGC 
CATGCTCATA ACATTTGTCA TCGAGATTTA AAACCGGAAA ACTTATTACT AGACGAGAGG 
AATAACATAA AAGTAGCAGA TTTTGGAATG GCTAGTCTGC AAGTGGAAGG GTCGATGTTG 
GAGACGAGTT GTGGATCTCC ACATTATGCA TGTCCAGAAG TTATTCGGGG TGAAAAGTAC 
GACGGTCGAA AAGCAGACGT TTGGAGTTGT GGCGTTATCC TCTACGCATT ATTAGTTGGT 
GCGCTTCCAT TTGATGATGA CAATCTCCGA AATCTTCTCG AAAAAGTGAA ACGTGGAGTT 
TTTCATATCC CACATTTCGT ACCTGCTGAT GTGCAGAGTT TACTGCGGGC AATGATAGAA 
GTAGACCCGG GGAAACGATA TTCCCTAGCC GACGTGTTCA AACATCCATG GGTATCCGGA 
ACAACAAAGG CAGATCCTGA ATTGGAGCTT CCAATGTCAC AAGTTGTGCA GACGCACGTA 
ATACCTGGTG AAGATTCGAT TGATCCGGAT GTCTTGAGGC ATATGAACTG CTTAGGATGT 
TTCAAAGACA AACAGAAGTT GATTAATGAA TTATTGTCGC CAAAACACAA CACTGAAAAG 
ATGGTATACT TTCTCCTTCT TGATCGAAAA CGACGGCGCC CTGCACAGGA AGACGACACA 
GAAATCGTTC TGCGCGGTGC CGCCCAGAAC AACGATCCGC CTAAAAAACG GACGGATTCT 
TCGAGAACAT CAAGATATCC CATGGGAAGC ATTGCTGATG GAAGTCCGAT CAATCCACGA 
AAAACGTATG GAAGGAATCA AAAGTCTGGA CGGCATTCAA GTTTGGGAGG TTCTCCTACA 
GAGTCACCGA GAAGTTCAAC TAGAGATCTG TTCGGTTCAT CGTCTAGCGG ATCTTACTCT 
GCAAGGGCTG GAGAAGATCG GGATAGAGGG CGATCTGCTT CAAGGTCCAC CAACAGCTAT 
CATTACTACA CTCAACCAGT CGATCCACAG ACTCTTGCGG AAGCAGCACG TCACGTACGT 
GACGCCCAGG AACGGAGGGA GAGCAGAGAC AGCGGTAGAG GGTCGTCTAG AAAAGAAAGC 
AAGGACAGAA GTGACAAAAG TGCCAGTAGT AGTAGTTGCA AAAATGATGC CTCGTCAACG 
TCTTCGGTGC CCCATAAGTA CAGCCCACCA TCAGTCATGT CGGAGAGTGT GGTAGTGTCT 
AGTAGTACGA TGAATTCTAC GAACTCGTCA ACAAATAGTT TGATAGCTGG TAACAGTCAG 
ACAAGCATTG GGTCGACTAG CGGTCCATGG CGATCAAAAC TCAACAACAT CAAAAACTCA 
TTCTTGGGAA CTCCGAGATT CCATCGGCGG AAAATGTCAA ATGGGACGGC AGAAAGTGAC 
AGTGAAGACA GCCAGATGAT TGACACAACT GACCTTGTCA AAAAGTCCTG GTTCGGATCT 
CTTGCTTCCA GTATGAGCGT CGAACGCGAT GACACCCACT GTGTGCCTGT TCAAGGGAAA 
ACATTGAACA GCATCAAAGC GGAGCTCATA CGGGCATTCC TTCAAATTCA TGAACTTAGT 
CACTCAGTAG TTGGTCAAAA TTGCTTTAGA GTAGAATATA AACGAGGTCC GACAGTTGGT 
GGAAGCGTTT TCAGCCGTGG AATCAAAATG AATGTTGATA TTATCCCATC TCCTCAACAA 
GTAGTGATAG CTGGAGAAAC TCCGACATAT GTAGTACAGT TTGTTCTGCT GGCGGGACCA 
GTTCGGAGGT TTAAACGACT TGTGGAACAC TTGTCAGCAA TTCTGCAAAA TTCTACTCAA 
CAAAGAGCTG ATCGACAACA ACAAGCAGCC CTAATGGTTC GACCAAGGAG GTTAAGTGAC 
TCCAGCGTTG GTAGTGCTTG TTCGGACAGT GAAAGCAATG CGAGCAGTAT TAATATGATA 
GCGCGGCATT CGGATAAAAC TGAAACAACT TCTGCCACGT CATCTGACCC TTACGGTCCA 
TCCCCGTCAA TGCGATCTGT TGGAAGTGGA ACAGGAATTA CATCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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sad-1 cDNA ORF clone, Caenorhabditis elegans

Gene

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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