FAM135A gene cDNA ORF clone, Bubalus bubalis(water buffalo)

mRNA	Protein	Name
XM_006051060.1	XP_006051122.1	protein FAM135A isoform X4
XM_025294942.1	XP_025150727.1	protein FAM135A isoform X1
XM_025294945.1	XP_025150730.1	protein FAM135A isoform X4
XM_006051057.1	XP_006051119.1	protein FAM135A isoform X1
XM_006051059.1	XP_006051121.1	protein FAM135A isoform X3
XM_025294946.1	XP_025150731.1	protein FAM135A isoform X5
XM_006051058.1	XP_006051120.1	protein FAM135A isoform X2
XM_025294943.1	XP_025150728.1	protein FAM135A isoform X2
XM_025294944.1	XP_025150729.1	protein FAM135A isoform X3
XM_025294941.1	XP_025150726.1	protein FAM135A isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu09038	XM_006051059.1 Latest version!	Bubalus bubalis family with sequence similarity 135, member A (FAM135A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBu09038	XM_025294944.1 Latest version!	Bubalus bubalis family with sequence similarity 135 member A (FAM135A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBu09039	XM_025294945.1 Latest version!	Bubalus bubalis family with sequence similarity 135 member A (FAM135A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBu09039	XM_006051060.1 Latest version!	Bubalus bubalis family with sequence similarity 135, member A (FAM135A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBu44226	XM_025294946.1 Latest version!	Bubalus bubalis family with sequence similarity 135 member A (FAM135A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu09036	XM_025294941.1 Latest version!	Bubalus bubalis family with sequence similarity 135 member A (FAM135A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBu09036	XM_006051057.1 Latest version!	Bubalus bubalis family with sequence similarity 135, member A (FAM135A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBu09036	XM_025294942.1 Latest version!	Bubalus bubalis family with sequence similarity 135 member A (FAM135A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBu09037	XM_006051058.1 Latest version!	Bubalus bubalis family with sequence similarity 135, member A (FAM135A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBu09037	XM_025294943.1 Latest version!	Bubalus bubalis family with sequence similarity 135 member A (FAM135A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

FAM135A ( XM_006051059.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006051121.1 Bubalus bubalis family with sequence similarity 135, member A (FAM135A), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu09038
Clone ID Related Accession (Same CDS sequence)	XM_006051059.1 , XM_025294944.1
Accession Version	XM_006051059.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4047bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	protein FAM135A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT 
CCGCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT 
TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG 
GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT 
GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA 
GATTATTCGG CAGATGATCT GAACGCCTTG CAGCTAATAA GCAGCCGCAC GCTGAAGCTG 
CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGCTAACG TCATGTTTGA TTATTTCCAC 
CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA 
AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA 
GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA 
TCACCAGATG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC 
CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT 
ACGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGCCAAAGC CAACATGCAG 
GTCCTTTATG AGCGCCTTCT CAGAAGAAAA CAGCCAGCAA CCCAGAAAGA CACCTGTCTC 
GAGGAAATGG ATGTAGAAGC TCGACTTACT GAATTATGTG AAGAAGTTAA GAAAATAGAA 
AATCCTGATG AACTGGCAGA ACTTATAAAT ATGAATCTTG CACAACTTTG CTCACTTTTA 
ATGGCTTTAT GGGGACAATT TCTGGAAGTT ATAACACTAC ATGAGGAACT AAGACTATTA 
CTAGCACAAG AGCACCATAC TCTGAGGGTG CGCAGGTTTT CTGAGGCCTT CTTTTGTTTT 
GAACATCCAA GAGAAGCTGC CATTGCATAC CAGGAACTTC ATGCTCAGAG TCATCTCCAG 
ATGTGCACCG CTATCAAAAA CACTTCCTTC TGCAGCTCTC TCCCACCCCT GCCTATTGAA 
TGTAGTGAAT TAGATGGAGA TCTCAATTCG TTACCTATTA TCTTTGAAGA TAGGTATTTA 
GATTCAGTTA CTGAAGGTAA ACTTGGTATC TCCCAGGATG ATAGTGAAAT TGCACAAGTG 
GAACACAGTG TGGCATCCAG AAGCTCATCA GACGATTGCC ATGATCGTCA GTCTGCCCCC 
ACTTCAGGAG TTAGGACAAT TGAAGTTAAG CCATGTAATA AAGACTGTTT CAGTGGAGAG 
AAAGTAACTG TTAAAATAGG ACCTTGGACA GAGCTTCAAC AAGATGAAAT ATTTGTGGAT 
AATTTACAAC TGCCCAACTT TGAGTCCTTA GACTCTAATG GTAAATCTAA ACCTATGGAA 
ATAACACTTG AAAAGGAAGC TTTGCAGGAA GCTAAGTGTC ATTCTGTCGG GGAATCGTTA 
GCTAAGTTAA GAAATAATCA ACCTGCTTCT ACAAGAGAAT ACCACCTTGT AGTGAGTGGA 
GACACCATTA AGTTACCAGA CATTAGTGCC ACGTGTGCCT CATCTCGGTT CTCAGACTCA 
GGTGTGGAAA GTGAGCCAAG CTCTGTTGCA GCACATCCAA ACCCTGACAT AGTCTTTGAA 
ACTGTACCAG GGCAAGGTCT TTTCATCAGT GAAAGGTTAT TTCCTCAGCT TTTGATGAAG 
CCTGACTGTA ATGTAAAGTT TTCACTAGGC AGTCACTGTA CTGAGAGTAC AAGTGCTTTC 
AGTGAGATCC AGTCGTCCTT AACCTCCATA AATTCTCTGC CTTCTGATGA CGAGCTGTCA 
CCTGATGAAA ATCCTAAGAA ATCTGCTGTA TCTGAATGCC ACCTAAGTGA TAGCAAAACT 
GTGTTAAATC TAGGAACAAC TGATATGCCA AAATGTGACG ATAGGAAAAA GTCAAGTATT 
ATTTTGCAGC AGCAGAGTGT CATATTTTCA GGGCATTCGG ACAATGAAAC TATAGAAATA 
CATTCCTTAA ATTCAAGCAC TAAAGACCCT TTACAATTTG TTTTTTCAGA TGAAGATACT 
TCTAGTGATG TGAAAAGTAG TTGCAGCTCC AAACCTAACT TGGACACTGT GTGTAAAGAC 
TCCCAGAGTC CTGATAAATC CAGTGACTCT GTAGGAACCA CAGTTCCGTT AAGTTCAGAA 
CTGGCTTGTT CAGGCACCCC TTGTGTCGTT TCAGGTTCCG TTTCCACCAG GGCAGATAGC 
AGTGAAGATA GAACTGTGAA AAGAAAAAAC AGTGATGCAT TAAATCCCAA ACAAATGTGT 
TCAGAAGCCC CTGCAGTTAC AGATGAAACT TACCTGGGTA CAGGTGATGC TTTTTCAGCC 
AGTCCTGATA TGGTAAAGCA AGGGCTTGTG GAAAATTATT TTGGCTGTCA AAGCAGTACG 
GATGTTTCTG ACACATGGGC TATTAGCTAC AGCCATTCAG TTAACCCTCA GAAAGAAACT 
TCTGGGAAAG AAATGAGTAG TCCTCAGCGG GATCAGGGTA AAGATGAGGA AGAGGAAGAG 
CAGGATCAAC AAATGGTTCA AAACGGGTAT TATGAGGAGG CAGATTACTC CTCTCTGGAG 
GCCGCGGGAG ATGCTCACTG TGCAGACGGA GCTGACCTGG GAGGAGAAAG ACTTGCCAAG 
TCTGAAAGAC TGGCCGGCGA CTATCTGAGC GATGGCATAA CCATGCCTAC TGTCTGTACT 
TCTGGCTGTC TGTCCTTCCC GTCTGCACCA CGAGAGTCCC CTTGTGGCGT TAAGTACTCT 
TCTAAGAGTA AATGTGACGC TGTTACCAAG CAGCCGAGCA GCGCCTCCCA CAGCTGCACC 
TCATCGCTGT CCTGGTACGA AAACTCGCCA AAGCCTCAGA TCCAAGCCTT CCTTCAGGCA 
AAAGATGAAT TGAAGCAACT TAAACTCCCC GGGTTCATGT ACAGCGATGT TCCTCTGCTG 
GCATCCTCAG TCCCTTATTT TTGCGTGGAG GAGGAGGAGG AGGACCATTA TGAAAATGGA 
GTGCATCTCA TTGTCTGTGT GCACGGCCTA GACGGTAACA GTGCAGATCT CCGATTAGTA 
AAAACTTACA TTGAACTTGG GCTGCCTGGG GAAAGAATTG ATTTTCTTAT GTCTGAAAGA 
AATCAGAATG ATACTTTTGC TGATTTTGAT AGCATGACTG ATCGCCTTTT GGATGAGATA 
ATACAATATA TTCAGATATA TAGTCTAACA GTCTCAAAAA TAAGCTTTAT TGGACATTCT 
TTGGGCAATT TAATAATCCG TTCAGTGCTT ACAAGACCAC GGTTTAAATA TTACCTCAGC 
AGGCTTCATA CCTTTCTTTC ACTTTCTGGA CCTCACCTTG GTACACTCTA CAATAGCAGT 
GCTCTTGTTA ATACAGGTCT GTGGTTTATG CAGAAATGGA AAAAATCAGG TTCTCTTTTA 
CAATTGACAT GTCGTGATCA TTCAGACCCT CGCCAAACTT TCTTATACAA GCTTAGTAAT 
AAAGCAGGGC TTCACTACTT CAGAAATGTT GTCCTAGTGG GATCTCTGCA AGATCGCTAT 
GTTCCTTATC ATTCTGCCCG CATTGAAATG TGCAAGACAG CTTTGAAGGA CAAACAGTCA 
GGACAGATCT ATTCAGAAAT GATCCACAAC TTACTTCGCC CGGTTCTGCA AAGCAAGGAC 
TGTAACTTGG TTCGATATAA TGTCATCAAT GCATTGCCCA ATACAGCTGA TTCACTCATT 
GGCAGAGCTG CACATATAGC TGTTCTGGAT TCAGAAATAT TTTTAGAGAA ATTCTTTCTG 
GTTGCTGCCC TCAAATATTT CCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006051121.1
CDS	225..4271
Translation	MTEVQAMVEFSVELNKFYNVDLFQRGFYQIRASMKIPPRVPHRVEASLLHAAGMTLAFPASVHDSLICSKTFQILYKNEEVVLNDVMIFKVKMLLDEKKIEETLEEMNFLLSLDLHFTDGDYSADDLNALQLISSRTLKLHFSLQRGLHHHANVMFDYFHLSVVSVTVHASLVALHQPLISFPRPVKTTWLNRNAPAQNKDSVIPTLESVVFGINYTKQLSPDGCSFVIADSFLRHAYRFHYTLCAALLLAFKGLHSYFITVTEELPSCQKLELAKANMQVLYERLLRRKQPATQKDTCLEEMDVEARLTELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVITLHEELRLLLAQEHHTLRVRRFSEAFFCFEHPREAAIAYQELHAQSHLQMCTAIKNTSFCSSLPPLPIECSELDGDLNSLPIIFEDRYLDSVTEGKLGISQDDSEIAQVEHSVASRSSSDDCHDRQSAPTSGVRTIEVKPCNKDCFSGEKVTVKIGPWTELQQDEIFVDNLQLPNFESLDSNGKSKPMEITLEKEALQEAKCHSVGESLAKLRNNQPASTREYHLVVSGDTIKLPDISATCASSRFSDSGVESEPSSVAAHPNPDIVFETVPGQGLFISERLFPQLLMKPDCNVKFSLGSHCTESTSAFSEIQSSLTSINSLPSDDELSPDENPKKSAVSECHLSDSKTVLNLGTTDMPKCDDRKKSSIILQQQSVIFSGHSDNETIEIHSLNSSTKDPLQFVFSDEDTSSDVKSSCSSKPNLDTVCKDSQSPDKSSDSVGTTVPLSSELACSGTPCVVSGSVSTRADSSEDRTVKRKNSDALNPKQMCSEAPAVTDETYLGTGDAFSASPDMVKQGLVENYFGCQSSTDVSDTWAISYSHSVNPQKETSGKEMSSPQRDQGKDEEEEEQDQQMVQNGYYEEADYSSLEAAGDAHCADGADLGGERLAKSERLAGDYLSDGITMPTVCTSGCLSFPSAPRESPCGVKYSSKSKCDAVTKQPSSASHSCTSSLSWYENSPKPQIQAFLQAKDELKQLKLPGFMYSDVPLLASSVPYFCVEEEEEDHYENGVHLIVCVHGLDGNSADLRLVKTYIELGLPGERIDFLMSERNQNDTFADFDSMTDRLLDEIIQYIQIYSLTVSKISFIGHSLGNLIIRSVLTRPRFKYYLSRLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSNKAGLHYFRNVVLVGSLQDRYVPYHSARIEMCKTALKDKQSGQIYSEMIHNLLRPVLQSKDCNLVRYNVINALPNTADSLIGRAAHIAVLDSEIFLEKFFLVAALKYFQ

Target ORF information:

RefSeq Version	XM_006051059.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis family with sequence similarity 135, member A (FAM135A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006051059.1

ORF Insert Sequence:

ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT 
CCGCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT 
TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG 
GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT 
GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA 
GATTATTCGG CAGATGATCT GAACGCCTTG CAGCTAATAA GCAGCCGCAC GCTGAAGCTG 
CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGCTAACG TCATGTTTGA TTATTTCCAC 
CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA 
AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA 
GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA 
TCACCAGATG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC 
CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT 
ACGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGCCAAAGC CAACATGCAG 
GTCCTTTATG AGCGCCTTCT CAGAAGAAAA CAGCCAGCAA CCCAGAAAGA CACCTGTCTC 
GAGGAAATGG ATGTAGAAGC TCGACTTACT GAATTATGTG AAGAAGTTAA GAAAATAGAA 
AATCCTGATG AACTGGCAGA ACTTATAAAT ATGAATCTTG CACAACTTTG CTCACTTTTA 
ATGGCTTTAT GGGGACAATT TCTGGAAGTT ATAACACTAC ATGAGGAACT AAGACTATTA 
CTAGCACAAG AGCACCATAC TCTGAGGGTG CGCAGGTTTT CTGAGGCCTT CTTTTGTTTT 
GAACATCCAA GAGAAGCTGC CATTGCATAC CAGGAACTTC ATGCTCAGAG TCATCTCCAG 
ATGTGCACCG CTATCAAAAA CACTTCCTTC TGCAGCTCTC TCCCACCCCT GCCTATTGAA 
TGTAGTGAAT TAGATGGAGA TCTCAATTCG TTACCTATTA TCTTTGAAGA TAGGTATTTA 
GATTCAGTTA CTGAAGGTAA ACTTGGTATC TCCCAGGATG ATAGTGAAAT TGCACAAGTG 
GAACACAGTG TGGCATCCAG AAGCTCATCA GACGATTGCC ATGATCGTCA GTCTGCCCCC 
ACTTCAGGAG TTAGGACAAT TGAAGTTAAG CCATGTAATA AAGACTGTTT CAGTGGAGAG 
AAAGTAACTG TTAAAATAGG ACCTTGGACA GAGCTTCAAC AAGATGAAAT ATTTGTGGAT 
AATTTACAAC TGCCCAACTT TGAGTCCTTA GACTCTAATG GTAAATCTAA ACCTATGGAA 
ATAACACTTG AAAAGGAAGC TTTGCAGGAA GCTAAGTGTC ATTCTGTCGG GGAATCGTTA 
GCTAAGTTAA GAAATAATCA ACCTGCTTCT ACAAGAGAAT ACCACCTTGT AGTGAGTGGA 
GACACCATTA AGTTACCAGA CATTAGTGCC ACGTGTGCCT CATCTCGGTT CTCAGACTCA 
GGTGTGGAAA GTGAGCCAAG CTCTGTTGCA GCACATCCAA ACCCTGACAT AGTCTTTGAA 
ACTGTACCAG GGCAAGGTCT TTTCATCAGT GAAAGGTTAT TTCCTCAGCT TTTGATGAAG 
CCTGACTGTA ATGTAAAGTT TTCACTAGGC AGTCACTGTA CTGAGAGTAC AAGTGCTTTC 
AGTGAGATCC AGTCGTCCTT AACCTCCATA AATTCTCTGC CTTCTGATGA CGAGCTGTCA 
CCTGATGAAA ATCCTAAGAA ATCTGCTGTA TCTGAATGCC ACCTAAGTGA TAGCAAAACT 
GTGTTAAATC TAGGAACAAC TGATATGCCA AAATGTGACG ATAGGAAAAA GTCAAGTATT 
ATTTTGCAGC AGCAGAGTGT CATATTTTCA GGGCATTCGG ACAATGAAAC TATAGAAATA 
CATTCCTTAA ATTCAAGCAC TAAAGACCCT TTACAATTTG TTTTTTCAGA TGAAGATACT 
TCTAGTGATG TGAAAAGTAG TTGCAGCTCC AAACCTAACT TGGACACTGT GTGTAAAGAC 
TCCCAGAGTC CTGATAAATC CAGTGACTCT GTAGGAACCA CAGTTCCGTT AAGTTCAGAA 
CTGGCTTGTT CAGGCACCCC TTGTGTCGTT TCAGGTTCCG TTTCCACCAG GGCAGATAGC 
AGTGAAGATA GAACTGTGAA AAGAAAAAAC AGTGATGCAT TAAATCCCAA ACAAATGTGT 
TCAGAAGCCC CTGCAGTTAC AGATGAAACT TACCTGGGTA CAGGTGATGC TTTTTCAGCC 
AGTCCTGATA TGGTAAAGCA AGGGCTTGTG GAAAATTATT TTGGCTGTCA AAGCAGTACG 
GATGTTTCTG ACACATGGGC TATTAGCTAC AGCCATTCAG TTAACCCTCA GAAAGAAACT 
TCTGGGAAAG AAATGAGTAG TCCTCAGCGG GATCAGGGTA AAGATGAGGA AGAGGAAGAG 
CAGGATCAAC AAATGGTTCA AAACGGGTAT TATGAGGAGG CAGATTACTC CTCTCTGGAG 
GCCGCGGGAG ATGCTCACTG TGCAGACGGA GCTGACCTGG GAGGAGAAAG ACTTGCCAAG 
TCTGAAAGAC TGGCCGGCGA CTATCTGAGC GATGGCATAA CCATGCCTAC TGTCTGTACT 
TCTGGCTGTC TGTCCTTCCC GTCTGCACCA CGAGAGTCCC CTTGTGGCGT TAAGTACTCT 
TCTAAGAGTA AATGTGACGC TGTTACCAAG CAGCCGAGCA GCGCCTCCCA CAGCTGCACC 
TCATCGCTGT CCTGGTACGA AAACTCGCCA AAGCCTCAGA TCCAAGCCTT CCTTCAGGCA 
AAAGATGAAT TGAAGCAACT TAAACTCCCC GGGTTCATGT ACAGCGATGT TCCTCTGCTG 
GCATCCTCAG TCCCTTATTT TTGCGTGGAG GAGGAGGAGG AGGACCATTA TGAAAATGGA 
GTGCATCTCA TTGTCTGTGT GCACGGCCTA GACGGTAACA GTGCAGATCT CCGATTAGTA 
AAAACTTACA TTGAACTTGG GCTGCCTGGG GAAAGAATTG ATTTTCTTAT GTCTGAAAGA 
AATCAGAATG ATACTTTTGC TGATTTTGAT AGCATGACTG ATCGCCTTTT GGATGAGATA 
ATACAATATA TTCAGATATA TAGTCTAACA GTCTCAAAAA TAAGCTTTAT TGGACATTCT 
TTGGGCAATT TAATAATCCG TTCAGTGCTT ACAAGACCAC GGTTTAAATA TTACCTCAGC 
AGGCTTCATA CCTTTCTTTC ACTTTCTGGA CCTCACCTTG GTACACTCTA CAATAGCAGT 
GCTCTTGTTA ATACAGGTCT GTGGTTTATG CAGAAATGGA AAAAATCAGG TTCTCTTTTA 
CAATTGACAT GTCGTGATCA TTCAGACCCT CGCCAAACTT TCTTATACAA GCTTAGTAAT 
AAAGCAGGGC TTCACTACTT CAGAAATGTT GTCCTAGTGG GATCTCTGCA AGATCGCTAT 
GTTCCTTATC ATTCTGCCCG CATTGAAATG TGCAAGACAG CTTTGAAGGA CAAACAGTCA 
GGACAGATCT ATTCAGAAAT GATCCACAAC TTACTTCGCC CGGTTCTGCA AAGCAAGGAC 
TGTAACTTGG TTCGATATAA TGTCATCAAT GCATTGCCCA ATACAGCTGA TTCACTCATT 
GGCAGAGCTG CACATATAGC TGTTCTGGAT TCAGAAATAT TTTTAGAGAA ATTCTTTCTG 
GTTGCTGCCC TCAAATATTT CCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FAM135A ( XM_025294944.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025150729.1 Bubalus bubalis family with sequence similarity 135 member A (FAM135A), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu09038
Clone ID Related Accession (Same CDS sequence)	XM_006051059.1 , XM_025294944.1
Accession Version	XM_025294944.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4047bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	protein FAM135A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037554.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT 
CCGCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT 
TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG 
GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT 
GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA 
GATTATTCGG CAGATGATCT GAACGCCTTG CAGCTAATAA GCAGCCGCAC GCTGAAGCTG 
CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGCTAACG TCATGTTTGA TTATTTCCAC 
CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA 
AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA 
GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA 
TCACCAGATG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC 
CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT 
ACGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGCCAAAGC CAACATGCAG 
GTCCTTTATG AGCGCCTTCT CAGAAGAAAA CAGCCAGCAA CCCAGAAAGA CACCTGTCTC 
GAGGAAATGG ATGTAGAAGC TCGACTTACT GAATTATGTG AAGAAGTTAA GAAAATAGAA 
AATCCTGATG AACTGGCAGA ACTTATAAAT ATGAATCTTG CACAACTTTG CTCACTTTTA 
ATGGCTTTAT GGGGACAATT TCTGGAAGTT ATAACACTAC ATGAGGAACT AAGACTATTA 
CTAGCACAAG AGCACCATAC TCTGAGGGTG CGCAGGTTTT CTGAGGCCTT CTTTTGTTTT 
GAACATCCAA GAGAAGCTGC CATTGCATAC CAGGAACTTC ATGCTCAGAG TCATCTCCAG 
ATGTGCACCG CTATCAAAAA CACTTCCTTC TGCAGCTCTC TCCCACCCCT GCCTATTGAA 
TGTAGTGAAT TAGATGGAGA TCTCAATTCG TTACCTATTA TCTTTGAAGA TAGGTATTTA 
GATTCAGTTA CTGAAGGTAA ACTTGGTATC TCCCAGGATG ATAGTGAAAT TGCACAAGTG 
GAACACAGTG TGGCATCCAG AAGCTCATCA GACGATTGCC ATGATCGTCA GTCTGCCCCC 
ACTTCAGGAG TTAGGACAAT TGAAGTTAAG CCATGTAATA AAGACTGTTT CAGTGGAGAG 
AAAGTAACTG TTAAAATAGG ACCTTGGACA GAGCTTCAAC AAGATGAAAT ATTTGTGGAT 
AATTTACAAC TGCCCAACTT TGAGTCCTTA GACTCTAATG GTAAATCTAA ACCTATGGAA 
ATAACACTTG AAAAGGAAGC TTTGCAGGAA GCTAAGTGTC ATTCTGTCGG GGAATCGTTA 
GCTAAGTTAA GAAATAATCA ACCTGCTTCT ACAAGAGAAT ACCACCTTGT AGTGAGTGGA 
GACACCATTA AGTTACCAGA CATTAGTGCC ACGTGTGCCT CATCTCGGTT CTCAGACTCA 
GGTGTGGAAA GTGAGCCAAG CTCTGTTGCA GCACATCCAA ACCCTGACAT AGTCTTTGAA 
ACTGTACCAG GGCAAGGTCT TTTCATCAGT GAAAGGTTAT TTCCTCAGCT TTTGATGAAG 
CCTGACTGTA ATGTAAAGTT TTCACTAGGC AGTCACTGTA CTGAGAGTAC AAGTGCTTTC 
AGTGAGATCC AGTCGTCCTT AACCTCCATA AATTCTCTGC CTTCTGATGA CGAGCTGTCA 
CCTGATGAAA ATCCTAAGAA ATCTGCTGTA TCTGAATGCC ACCTAAGTGA TAGCAAAACT 
GTGTTAAATC TAGGAACAAC TGATATGCCA AAATGTGACG ATAGGAAAAA GTCAAGTATT 
ATTTTGCAGC AGCAGAGTGT CATATTTTCA GGGCATTCGG ACAATGAAAC TATAGAAATA 
CATTCCTTAA ATTCAAGCAC TAAAGACCCT TTACAATTTG TTTTTTCAGA TGAAGATACT 
TCTAGTGATG TGAAAAGTAG TTGCAGCTCC AAACCTAACT TGGACACTGT GTGTAAAGAC 
TCCCAGAGTC CTGATAAATC CAGTGACTCT GTAGGAACCA CAGTTCCGTT AAGTTCAGAA 
CTGGCTTGTT CAGGCACCCC TTGTGTCGTT TCAGGTTCCG TTTCCACCAG GGCAGATAGC 
AGTGAAGATA GAACTGTGAA AAGAAAAAAC AGTGATGCAT TAAATCCCAA ACAAATGTGT 
TCAGAAGCCC CTGCAGTTAC AGATGAAACT TACCTGGGTA CAGGTGATGC TTTTTCAGCC 
AGTCCTGATA TGGTAAAGCA AGGGCTTGTG GAAAATTATT TTGGCTGTCA AAGCAGTACG 
GATGTTTCTG ACACATGGGC TATTAGCTAC AGCCATTCAG TTAACCCTCA GAAAGAAACT 
TCTGGGAAAG AAATGAGTAG TCCTCAGCGG GATCAGGGTA AAGATGAGGA AGAGGAAGAG 
CAGGATCAAC AAATGGTTCA AAACGGGTAT TATGAGGAGG CAGATTACTC CTCTCTGGAG 
GCCGCGGGAG ATGCTCACTG TGCAGACGGA GCTGACCTGG GAGGAGAAAG ACTTGCCAAG 
TCTGAAAGAC TGGCCGGCGA CTATCTGAGC GATGGCATAA CCATGCCTAC TGTCTGTACT 
TCTGGCTGTC TGTCCTTCCC GTCTGCACCA CGAGAGTCCC CTTGTGGCGT TAAGTACTCT 
TCTAAGAGTA AATGTGACGC TGTTACCAAG CAGCCGAGCA GCGCCTCCCA CAGCTGCACC 
TCATCGCTGT CCTGGTACGA AAACTCGCCA AAGCCTCAGA TCCAAGCCTT CCTTCAGGCA 
AAAGATGAAT TGAAGCAACT TAAACTCCCC GGGTTCATGT ACAGCGATGT TCCTCTGCTG 
GCATCCTCAG TCCCTTATTT TTGCGTGGAG GAGGAGGAGG AGGACCATTA TGAAAATGGA 
GTGCATCTCA TTGTCTGTGT GCACGGCCTA GACGGTAACA GTGCAGATCT CCGATTAGTA 
AAAACTTACA TTGAACTTGG GCTGCCTGGG GAAAGAATTG ATTTTCTTAT GTCTGAAAGA 
AATCAGAATG ATACTTTTGC TGATTTTGAT AGCATGACTG ATCGCCTTTT GGATGAGATA 
ATACAATATA TTCAGATATA TAGTCTAACA GTCTCAAAAA TAAGCTTTAT TGGACATTCT 
TTGGGCAATT TAATAATCCG TTCAGTGCTT ACAAGACCAC GGTTTAAATA TTACCTCAGC 
AGGCTTCATA CCTTTCTTTC ACTTTCTGGA CCTCACCTTG GTACACTCTA CAATAGCAGT 
GCTCTTGTTA ATACAGGTCT GTGGTTTATG CAGAAATGGA AAAAATCAGG TTCTCTTTTA 
CAATTGACAT GTCGTGATCA TTCAGACCCT CGCCAAACTT TCTTATACAA GCTTAGTAAT 
AAAGCAGGGC TTCACTACTT CAGAAATGTT GTCCTAGTGG GATCTCTGCA AGATCGCTAT 
GTTCCTTATC ATTCTGCCCG CATTGAAATG TGCAAGACAG CTTTGAAGGA CAAACAGTCA 
GGACAGATCT ATTCAGAAAT GATCCACAAC TTACTTCGCC CGGTTCTGCA AAGCAAGGAC 
TGTAACTTGG TTCGATATAA TGTCATCAAT GCATTGCCCA ATACAGCTGA TTCACTCATT 
GGCAGAGCTG CACATATAGC TGTTCTGGAT TCAGAAATAT TTTTAGAGAA ATTCTTTCTG 
GTTGCTGCCC TCAAATATTT CCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025150729.1
CDS	375..4421
Translation	MTEVQAMVEFSVELNKFYNVDLFQRGFYQIRASMKIPPRVPHRVEASLLHAAGMTLAFPASVHDSLICSKTFQILYKNEEVVLNDVMIFKVKMLLDEKKIEETLEEMNFLLSLDLHFTDGDYSADDLNALQLISSRTLKLHFSLQRGLHHHANVMFDYFHLSVVSVTVHASLVALHQPLISFPRPVKTTWLNRNAPAQNKDSVIPTLESVVFGINYTKQLSPDGCSFVIADSFLRHAYRFHYTLCAALLLAFKGLHSYFITVTEELPSCQKLELAKANMQVLYERLLRRKQPATQKDTCLEEMDVEARLTELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVITLHEELRLLLAQEHHTLRVRRFSEAFFCFEHPREAAIAYQELHAQSHLQMCTAIKNTSFCSSLPPLPIECSELDGDLNSLPIIFEDRYLDSVTEGKLGISQDDSEIAQVEHSVASRSSSDDCHDRQSAPTSGVRTIEVKPCNKDCFSGEKVTVKIGPWTELQQDEIFVDNLQLPNFESLDSNGKSKPMEITLEKEALQEAKCHSVGESLAKLRNNQPASTREYHLVVSGDTIKLPDISATCASSRFSDSGVESEPSSVAAHPNPDIVFETVPGQGLFISERLFPQLLMKPDCNVKFSLGSHCTESTSAFSEIQSSLTSINSLPSDDELSPDENPKKSAVSECHLSDSKTVLNLGTTDMPKCDDRKKSSIILQQQSVIFSGHSDNETIEIHSLNSSTKDPLQFVFSDEDTSSDVKSSCSSKPNLDTVCKDSQSPDKSSDSVGTTVPLSSELACSGTPCVVSGSVSTRADSSEDRTVKRKNSDALNPKQMCSEAPAVTDETYLGTGDAFSASPDMVKQGLVENYFGCQSSTDVSDTWAISYSHSVNPQKETSGKEMSSPQRDQGKDEEEEEQDQQMVQNGYYEEADYSSLEAAGDAHCADGADLGGERLAKSERLAGDYLSDGITMPTVCTSGCLSFPSAPRESPCGVKYSSKSKCDAVTKQPSSASHSCTSSLSWYENSPKPQIQAFLQAKDELKQLKLPGFMYSDVPLLASSVPYFCVEEEEEDHYENGVHLIVCVHGLDGNSADLRLVKTYIELGLPGERIDFLMSERNQNDTFADFDSMTDRLLDEIIQYIQIYSLTVSKISFIGHSLGNLIIRSVLTRPRFKYYLSRLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSNKAGLHYFRNVVLVGSLQDRYVPYHSARIEMCKTALKDKQSGQIYSEMIHNLLRPVLQSKDCNLVRYNVINALPNTADSLIGRAAHIAVLDSEIFLEKFFLVAALKYFQ

Target ORF information:

RefSeq Version	XM_025294944.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis family with sequence similarity 135 member A (FAM135A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025294944.1

ORF Insert Sequence:

ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT 
CCGCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT 
TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG 
GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT 
GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA 
GATTATTCGG CAGATGATCT GAACGCCTTG CAGCTAATAA GCAGCCGCAC GCTGAAGCTG 
CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGCTAACG TCATGTTTGA TTATTTCCAC 
CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA 
AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA 
GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA 
TCACCAGATG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC 
CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT 
ACGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGCCAAAGC CAACATGCAG 
GTCCTTTATG AGCGCCTTCT CAGAAGAAAA CAGCCAGCAA CCCAGAAAGA CACCTGTCTC 
GAGGAAATGG ATGTAGAAGC TCGACTTACT GAATTATGTG AAGAAGTTAA GAAAATAGAA 
AATCCTGATG AACTGGCAGA ACTTATAAAT ATGAATCTTG CACAACTTTG CTCACTTTTA 
ATGGCTTTAT GGGGACAATT TCTGGAAGTT ATAACACTAC ATGAGGAACT AAGACTATTA 
CTAGCACAAG AGCACCATAC TCTGAGGGTG CGCAGGTTTT CTGAGGCCTT CTTTTGTTTT 
GAACATCCAA GAGAAGCTGC CATTGCATAC CAGGAACTTC ATGCTCAGAG TCATCTCCAG 
ATGTGCACCG CTATCAAAAA CACTTCCTTC TGCAGCTCTC TCCCACCCCT GCCTATTGAA 
TGTAGTGAAT TAGATGGAGA TCTCAATTCG TTACCTATTA TCTTTGAAGA TAGGTATTTA 
GATTCAGTTA CTGAAGGTAA ACTTGGTATC TCCCAGGATG ATAGTGAAAT TGCACAAGTG 
GAACACAGTG TGGCATCCAG AAGCTCATCA GACGATTGCC ATGATCGTCA GTCTGCCCCC 
ACTTCAGGAG TTAGGACAAT TGAAGTTAAG CCATGTAATA AAGACTGTTT CAGTGGAGAG 
AAAGTAACTG TTAAAATAGG ACCTTGGACA GAGCTTCAAC AAGATGAAAT ATTTGTGGAT 
AATTTACAAC TGCCCAACTT TGAGTCCTTA GACTCTAATG GTAAATCTAA ACCTATGGAA 
ATAACACTTG AAAAGGAAGC TTTGCAGGAA GCTAAGTGTC ATTCTGTCGG GGAATCGTTA 
GCTAAGTTAA GAAATAATCA ACCTGCTTCT ACAAGAGAAT ACCACCTTGT AGTGAGTGGA 
GACACCATTA AGTTACCAGA CATTAGTGCC ACGTGTGCCT CATCTCGGTT CTCAGACTCA 
GGTGTGGAAA GTGAGCCAAG CTCTGTTGCA GCACATCCAA ACCCTGACAT AGTCTTTGAA 
ACTGTACCAG GGCAAGGTCT TTTCATCAGT GAAAGGTTAT TTCCTCAGCT TTTGATGAAG 
CCTGACTGTA ATGTAAAGTT TTCACTAGGC AGTCACTGTA CTGAGAGTAC AAGTGCTTTC 
AGTGAGATCC AGTCGTCCTT AACCTCCATA AATTCTCTGC CTTCTGATGA CGAGCTGTCA 
CCTGATGAAA ATCCTAAGAA ATCTGCTGTA TCTGAATGCC ACCTAAGTGA TAGCAAAACT 
GTGTTAAATC TAGGAACAAC TGATATGCCA AAATGTGACG ATAGGAAAAA GTCAAGTATT 
ATTTTGCAGC AGCAGAGTGT CATATTTTCA GGGCATTCGG ACAATGAAAC TATAGAAATA 
CATTCCTTAA ATTCAAGCAC TAAAGACCCT TTACAATTTG TTTTTTCAGA TGAAGATACT 
TCTAGTGATG TGAAAAGTAG TTGCAGCTCC AAACCTAACT TGGACACTGT GTGTAAAGAC 
TCCCAGAGTC CTGATAAATC CAGTGACTCT GTAGGAACCA CAGTTCCGTT AAGTTCAGAA 
CTGGCTTGTT CAGGCACCCC TTGTGTCGTT TCAGGTTCCG TTTCCACCAG GGCAGATAGC 
AGTGAAGATA GAACTGTGAA AAGAAAAAAC AGTGATGCAT TAAATCCCAA ACAAATGTGT 
TCAGAAGCCC CTGCAGTTAC AGATGAAACT TACCTGGGTA CAGGTGATGC TTTTTCAGCC 
AGTCCTGATA TGGTAAAGCA AGGGCTTGTG GAAAATTATT TTGGCTGTCA AAGCAGTACG 
GATGTTTCTG ACACATGGGC TATTAGCTAC AGCCATTCAG TTAACCCTCA GAAAGAAACT 
TCTGGGAAAG AAATGAGTAG TCCTCAGCGG GATCAGGGTA AAGATGAGGA AGAGGAAGAG 
CAGGATCAAC AAATGGTTCA AAACGGGTAT TATGAGGAGG CAGATTACTC CTCTCTGGAG 
GCCGCGGGAG ATGCTCACTG TGCAGACGGA GCTGACCTGG GAGGAGAAAG ACTTGCCAAG 
TCTGAAAGAC TGGCCGGCGA CTATCTGAGC GATGGCATAA CCATGCCTAC TGTCTGTACT 
TCTGGCTGTC TGTCCTTCCC GTCTGCACCA CGAGAGTCCC CTTGTGGCGT TAAGTACTCT 
TCTAAGAGTA AATGTGACGC TGTTACCAAG CAGCCGAGCA GCGCCTCCCA CAGCTGCACC 
TCATCGCTGT CCTGGTACGA AAACTCGCCA AAGCCTCAGA TCCAAGCCTT CCTTCAGGCA 
AAAGATGAAT TGAAGCAACT TAAACTCCCC GGGTTCATGT ACAGCGATGT TCCTCTGCTG 
GCATCCTCAG TCCCTTATTT TTGCGTGGAG GAGGAGGAGG AGGACCATTA TGAAAATGGA 
GTGCATCTCA TTGTCTGTGT GCACGGCCTA GACGGTAACA GTGCAGATCT CCGATTAGTA 
AAAACTTACA TTGAACTTGG GCTGCCTGGG GAAAGAATTG ATTTTCTTAT GTCTGAAAGA 
AATCAGAATG ATACTTTTGC TGATTTTGAT AGCATGACTG ATCGCCTTTT GGATGAGATA 
ATACAATATA TTCAGATATA TAGTCTAACA GTCTCAAAAA TAAGCTTTAT TGGACATTCT 
TTGGGCAATT TAATAATCCG TTCAGTGCTT ACAAGACCAC GGTTTAAATA TTACCTCAGC 
AGGCTTCATA CCTTTCTTTC ACTTTCTGGA CCTCACCTTG GTACACTCTA CAATAGCAGT 
GCTCTTGTTA ATACAGGTCT GTGGTTTATG CAGAAATGGA AAAAATCAGG TTCTCTTTTA 
CAATTGACAT GTCGTGATCA TTCAGACCCT CGCCAAACTT TCTTATACAA GCTTAGTAAT 
AAAGCAGGGC TTCACTACTT CAGAAATGTT GTCCTAGTGG GATCTCTGCA AGATCGCTAT 
GTTCCTTATC ATTCTGCCCG CATTGAAATG TGCAAGACAG CTTTGAAGGA CAAACAGTCA 
GGACAGATCT ATTCAGAAAT GATCCACAAC TTACTTCGCC CGGTTCTGCA AAGCAAGGAC 
TGTAACTTGG TTCGATATAA TGTCATCAAT GCATTGCCCA ATACAGCTGA TTCACTCATT 
GGCAGAGCTG CACATATAGC TGTTCTGGAT TCAGAAATAT TTTTAGAGAA ATTCTTTCTG 
GTTGCTGCCC TCAAATATTT CCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FAM135A ( XM_006051060.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006051122.1 Bubalus bubalis family with sequence similarity 135, member A (FAM135A), transcript variant X4, mRNA.

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CloneID	OBu09039
Clone ID Related Accession (Same CDS sequence)	XM_006051060.1 , XM_025294945.1
Accession Version	XM_006051060.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	protein FAM135A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT 
CCGCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT 
TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG 
GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT 
GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA 
GATTATTCGG CAGATGATCT GAACGCCTTG CAGCTAATAA GCAGCCGCAC GCTGAAGCTG 
CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGCTAACG TCATGTTTGA TTATTTCCAC 
CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA 
AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA 
GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA 
TCACCAGATG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC 
CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT 
ACGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGAGGAAAT GGATGTAGAA 
GCTCGACTTA CTGAATTATG TGAAGAAGTT AAGAAAATAG AAAATCCTGA TGAACTGGCA 
GAACTTATAA ATATGAATCT TGCACAACTT TGCTCACTTT TAATGGCTTT ATGGGGACAA 
TTTCTGGAAG TTATAACACT ACATGAGGAA CTAAGACTAT TACTAGCACA AGAGCACCAT 
ACTCTGAGGG TGCGCAGGTT TTCTGAGGCC TTCTTTTGTT TTGAACATCC AAGAGAAGCT 
GCCATTGCAT ACCAGGAACT TCATGCTCAG AGTCATCTCC AGATGTGCAC CGCTATCAAA 
AACACTTCCT TCTGCAGCTC TCTCCCACCC CTGCCTATTG AATGTAGTGA ATTAGATGGA 
GATCTCAATT CGTTACCTAT TATCTTTGAA GATAGGTATT TAGATTCAGT TACTGAAGGT 
AAACTTGGTA TCTCCCAGGA TGATAGTGAA ATTGCACAAG TGGAACACAG TGTGGCATCC 
AGAAGCTCAT CAGACGATTG CCATGATCGT CAGTCTGCCC CCACTTCAGG AGTTAGGACA 
ATTGAAGTTA AGCCATGTAA TAAAGACTGT TTCAGTGGAG AGAAAGTAAC TGTTAAAATA 
GGACCTTGGA CAGAGCTTCA ACAAGATGAA ATATTTGTGG ATAATTTACA ACTGCCCAAC 
TTTGAGTCCT TAGACTCTAA TGGTAAATCT AAACCTATGG AAATAACACT TGAAAAGGAA 
GCTTTGCAGG AAGCTAAGTG TCATTCTGTC GGGGAATCGT TAGCTAAGTT AAGAAATAAT 
CAACCTGCTT CTACAAGAGA ATACCACCTT GTAGTGAGTG GAGACACCAT TAAGTTACCA 
GACATTAGTG CCACGTGTGC CTCATCTCGG TTCTCAGACT CAGGTGTGGA AAGTGAGCCA 
AGCTCTGTTG CAGCACATCC AAACCCTGAC ATAGTCTTTG AAACTGTACC AGGGCAAGGT 
CTTTTCATCA GTGAAAGGTT ATTTCCTCAG CTTTTGATGA AGCCTGACTG TAATGTAAAG 
TTTTCACTAG GCAGTCACTG TACTGAGAGT ACAAGTGCTT TCAGTGAGAT CCAGTCGTCC 
TTAACCTCCA TAAATTCTCT GCCTTCTGAT GACGAGCTGT CACCTGATGA AAATCCTAAG 
AAATCTGCTG TATCTGAATG CCACCTAAGT GATAGCAAAA CTGTGTTAAA TCTAGGAACA 
ACTGATATGC CAAAATGTGA CGATAGGAAA AAGTCAAGTA TTATTTTGCA GCAGCAGAGT 
GTCATATTTT CAGGGCATTC GGACAATGAA ACTATAGAAA TACATTCCTT AAATTCAAGC 
ACTAAAGACC CTTTACAATT TGTTTTTTCA GATGAAGATA CTTCTAGTGA TGTGAAAAGT 
AGTTGCAGCT CCAAACCTAA CTTGGACACT GTGTGTAAAG ACTCCCAGAG TCCTGATAAA 
TCCAGTGACT CTGTAGGAAC CACAGTTCCG TTAAGTTCAG AACTGGCTTG TTCAGGCACC 
CCTTGTGTCG TTTCAGGTTC CGTTTCCACC AGGGCAGATA GCAGTGAAGA TAGAACTGTG 
AAAAGAAAAA ACAGTGATGC ATTAAATCCC AAACAAATGT GTTCAGAAGC CCCTGCAGTT 
ACAGATGAAA CTTACCTGGG TACAGGTGAT GCTTTTTCAG CCAGTCCTGA TATGGTAAAG 
CAAGGGCTTG TGGAAAATTA TTTTGGCTGT CAAAGCAGTA CGGATGTTTC TGACACATGG 
GCTATTAGCT ACAGCCATTC AGTTAACCCT CAGAAAGAAA CTTCTGGGAA AGAAATGAGT 
AGTCCTCAGC GGGATCAGGG TAAAGATGAG GAAGAGGAAG AGCAGGATCA ACAAATGGTT 
CAAAACGGGT ATTATGAGGA GGCAGATTAC TCCTCTCTGG AGGCCGCGGG AGATGCTCAC 
TGTGCAGACG GAGCTGACCT GGGAGGAGAA AGACTTGCCA AGTCTGAAAG ACTGGCCGGC 
GACTATCTGA GCGATGGCAT AACCATGCCT ACTGTCTGTA CTTCTGGCTG TCTGTCCTTC 
CCGTCTGCAC CACGAGAGTC CCCTTGTGGC GTTAAGTACT CTTCTAAGAG TAAATGTGAC 
GCTGTTACCA AGCAGCCGAG CAGCGCCTCC CACAGCTGCA CCTCATCGCT GTCCTGGTAC 
GAAAACTCGC CAAAGCCTCA GATCCAAGCC TTCCTTCAGG CAAAAGATGA ATTGAAGCAA 
CTTAAACTCC CCGGGTTCAT GTACAGCGAT GTTCCTCTGC TGGCATCCTC AGTCCCTTAT 
TTTTGCGTGG AGGAGGAGGA GGAGGACCAT TATGAAAATG GAGTGCATCT CATTGTCTGT 
GTGCACGGCC TAGACGGTAA CAGTGCAGAT CTCCGATTAG TAAAAACTTA CATTGAACTT 
GGGCTGCCTG GGGAAAGAAT TGATTTTCTT ATGTCTGAAA GAAATCAGAA TGATACTTTT 
GCTGATTTTG ATAGCATGAC TGATCGCCTT TTGGATGAGA TAATACAATA TATTCAGATA 
TATAGTCTAA CAGTCTCAAA AATAAGCTTT ATTGGACATT CTTTGGGCAA TTTAATAATC 
CGTTCAGTGC TTACAAGACC ACGGTTTAAA TATTACCTCA GCAGGCTTCA TACCTTTCTT 
TCACTTTCTG GACCTCACCT TGGTACACTC TACAATAGCA GTGCTCTTGT TAATACAGGT 
CTGTGGTTTA TGCAGAAATG GAAAAAATCA GGTTCTCTTT TACAATTGAC ATGTCGTGAT 
CATTCAGACC CTCGCCAAAC TTTCTTATAC AAGCTTAGTA ATAAAGCAGG GCTTCACTAC 
TTCAGAAATG TTGTCCTAGT GGGATCTCTG CAAGATCGCT ATGTTCCTTA TCATTCTGCC 
CGCATTGAAA TGTGCAAGAC AGCTTTGAAG GACAAACAGT CAGGACAGAT CTATTCAGAA 
ATGATCCACA ACTTACTTCG CCCGGTTCTG CAAAGCAAGG ACTGTAACTT GGTTCGATAT 
AATGTCATCA ATGCATTGCC CAATACAGCT GATTCACTCA TTGGCAGAGC TGCACATATA 
GCTGTTCTGG ATTCAGAAAT ATTTTTAGAG AAATTCTTTC TGGTTGCTGC CCTCAAATAT 
TTCCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006051122.1
CDS	225..4193
Translation	MTEVQAMVEFSVELNKFYNVDLFQRGFYQIRASMKIPPRVPHRVEASLLHAAGMTLAFPASVHDSLICSKTFQILYKNEEVVLNDVMIFKVKMLLDEKKIEETLEEMNFLLSLDLHFTDGDYSADDLNALQLISSRTLKLHFSLQRGLHHHANVMFDYFHLSVVSVTVHASLVALHQPLISFPRPVKTTWLNRNAPAQNKDSVIPTLESVVFGINYTKQLSPDGCSFVIADSFLRHAYRFHYTLCAALLLAFKGLHSYFITVTEELPSCQKLELEEMDVEARLTELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVITLHEELRLLLAQEHHTLRVRRFSEAFFCFEHPREAAIAYQELHAQSHLQMCTAIKNTSFCSSLPPLPIECSELDGDLNSLPIIFEDRYLDSVTEGKLGISQDDSEIAQVEHSVASRSSSDDCHDRQSAPTSGVRTIEVKPCNKDCFSGEKVTVKIGPWTELQQDEIFVDNLQLPNFESLDSNGKSKPMEITLEKEALQEAKCHSVGESLAKLRNNQPASTREYHLVVSGDTIKLPDISATCASSRFSDSGVESEPSSVAAHPNPDIVFETVPGQGLFISERLFPQLLMKPDCNVKFSLGSHCTESTSAFSEIQSSLTSINSLPSDDELSPDENPKKSAVSECHLSDSKTVLNLGTTDMPKCDDRKKSSIILQQQSVIFSGHSDNETIEIHSLNSSTKDPLQFVFSDEDTSSDVKSSCSSKPNLDTVCKDSQSPDKSSDSVGTTVPLSSELACSGTPCVVSGSVSTRADSSEDRTVKRKNSDALNPKQMCSEAPAVTDETYLGTGDAFSASPDMVKQGLVENYFGCQSSTDVSDTWAISYSHSVNPQKETSGKEMSSPQRDQGKDEEEEEQDQQMVQNGYYEEADYSSLEAAGDAHCADGADLGGERLAKSERLAGDYLSDGITMPTVCTSGCLSFPSAPRESPCGVKYSSKSKCDAVTKQPSSASHSCTSSLSWYENSPKPQIQAFLQAKDELKQLKLPGFMYSDVPLLASSVPYFCVEEEEEDHYENGVHLIVCVHGLDGNSADLRLVKTYIELGLPGERIDFLMSERNQNDTFADFDSMTDRLLDEIIQYIQIYSLTVSKISFIGHSLGNLIIRSVLTRPRFKYYLSRLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSNKAGLHYFRNVVLVGSLQDRYVPYHSARIEMCKTALKDKQSGQIYSEMIHNLLRPVLQSKDCNLVRYNVINALPNTADSLIGRAAHIAVLDSEIFLEKFFLVAALKYFQ

Target ORF information:

RefSeq Version	XM_006051060.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis family with sequence similarity 135, member A (FAM135A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006051060.1

ORF Insert Sequence:

ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT 
CCGCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT 
TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG 
GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT 
GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA 
GATTATTCGG CAGATGATCT GAACGCCTTG CAGCTAATAA GCAGCCGCAC GCTGAAGCTG 
CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGCTAACG TCATGTTTGA TTATTTCCAC 
CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA 
AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA 
GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA 
TCACCAGATG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC 
CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT 
ACGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGAGGAAAT GGATGTAGAA 
GCTCGACTTA CTGAATTATG TGAAGAAGTT AAGAAAATAG AAAATCCTGA TGAACTGGCA 
GAACTTATAA ATATGAATCT TGCACAACTT TGCTCACTTT TAATGGCTTT ATGGGGACAA 
TTTCTGGAAG TTATAACACT ACATGAGGAA CTAAGACTAT TACTAGCACA AGAGCACCAT 
ACTCTGAGGG TGCGCAGGTT TTCTGAGGCC TTCTTTTGTT TTGAACATCC AAGAGAAGCT 
GCCATTGCAT ACCAGGAACT TCATGCTCAG AGTCATCTCC AGATGTGCAC CGCTATCAAA 
AACACTTCCT TCTGCAGCTC TCTCCCACCC CTGCCTATTG AATGTAGTGA ATTAGATGGA 
GATCTCAATT CGTTACCTAT TATCTTTGAA GATAGGTATT TAGATTCAGT TACTGAAGGT 
AAACTTGGTA TCTCCCAGGA TGATAGTGAA ATTGCACAAG TGGAACACAG TGTGGCATCC 
AGAAGCTCAT CAGACGATTG CCATGATCGT CAGTCTGCCC CCACTTCAGG AGTTAGGACA 
ATTGAAGTTA AGCCATGTAA TAAAGACTGT TTCAGTGGAG AGAAAGTAAC TGTTAAAATA 
GGACCTTGGA CAGAGCTTCA ACAAGATGAA ATATTTGTGG ATAATTTACA ACTGCCCAAC 
TTTGAGTCCT TAGACTCTAA TGGTAAATCT AAACCTATGG AAATAACACT TGAAAAGGAA 
GCTTTGCAGG AAGCTAAGTG TCATTCTGTC GGGGAATCGT TAGCTAAGTT AAGAAATAAT 
CAACCTGCTT CTACAAGAGA ATACCACCTT GTAGTGAGTG GAGACACCAT TAAGTTACCA 
GACATTAGTG CCACGTGTGC CTCATCTCGG TTCTCAGACT CAGGTGTGGA AAGTGAGCCA 
AGCTCTGTTG CAGCACATCC AAACCCTGAC ATAGTCTTTG AAACTGTACC AGGGCAAGGT 
CTTTTCATCA GTGAAAGGTT ATTTCCTCAG CTTTTGATGA AGCCTGACTG TAATGTAAAG 
TTTTCACTAG GCAGTCACTG TACTGAGAGT ACAAGTGCTT TCAGTGAGAT CCAGTCGTCC 
TTAACCTCCA TAAATTCTCT GCCTTCTGAT GACGAGCTGT CACCTGATGA AAATCCTAAG 
AAATCTGCTG TATCTGAATG CCACCTAAGT GATAGCAAAA CTGTGTTAAA TCTAGGAACA 
ACTGATATGC CAAAATGTGA CGATAGGAAA AAGTCAAGTA TTATTTTGCA GCAGCAGAGT 
GTCATATTTT CAGGGCATTC GGACAATGAA ACTATAGAAA TACATTCCTT AAATTCAAGC 
ACTAAAGACC CTTTACAATT TGTTTTTTCA GATGAAGATA CTTCTAGTGA TGTGAAAAGT 
AGTTGCAGCT CCAAACCTAA CTTGGACACT GTGTGTAAAG ACTCCCAGAG TCCTGATAAA 
TCCAGTGACT CTGTAGGAAC CACAGTTCCG TTAAGTTCAG AACTGGCTTG TTCAGGCACC 
CCTTGTGTCG TTTCAGGTTC CGTTTCCACC AGGGCAGATA GCAGTGAAGA TAGAACTGTG 
AAAAGAAAAA ACAGTGATGC ATTAAATCCC AAACAAATGT GTTCAGAAGC CCCTGCAGTT 
ACAGATGAAA CTTACCTGGG TACAGGTGAT GCTTTTTCAG CCAGTCCTGA TATGGTAAAG 
CAAGGGCTTG TGGAAAATTA TTTTGGCTGT CAAAGCAGTA CGGATGTTTC TGACACATGG 
GCTATTAGCT ACAGCCATTC AGTTAACCCT CAGAAAGAAA CTTCTGGGAA AGAAATGAGT 
AGTCCTCAGC GGGATCAGGG TAAAGATGAG GAAGAGGAAG AGCAGGATCA ACAAATGGTT 
CAAAACGGGT ATTATGAGGA GGCAGATTAC TCCTCTCTGG AGGCCGCGGG AGATGCTCAC 
TGTGCAGACG GAGCTGACCT GGGAGGAGAA AGACTTGCCA AGTCTGAAAG ACTGGCCGGC 
GACTATCTGA GCGATGGCAT AACCATGCCT ACTGTCTGTA CTTCTGGCTG TCTGTCCTTC 
CCGTCTGCAC CACGAGAGTC CCCTTGTGGC GTTAAGTACT CTTCTAAGAG TAAATGTGAC 
GCTGTTACCA AGCAGCCGAG CAGCGCCTCC CACAGCTGCA CCTCATCGCT GTCCTGGTAC 
GAAAACTCGC CAAAGCCTCA GATCCAAGCC TTCCTTCAGG CAAAAGATGA ATTGAAGCAA 
CTTAAACTCC CCGGGTTCAT GTACAGCGAT GTTCCTCTGC TGGCATCCTC AGTCCCTTAT 
TTTTGCGTGG AGGAGGAGGA GGAGGACCAT TATGAAAATG GAGTGCATCT CATTGTCTGT 
GTGCACGGCC TAGACGGTAA CAGTGCAGAT CTCCGATTAG TAAAAACTTA CATTGAACTT 
GGGCTGCCTG GGGAAAGAAT TGATTTTCTT ATGTCTGAAA GAAATCAGAA TGATACTTTT 
GCTGATTTTG ATAGCATGAC TGATCGCCTT TTGGATGAGA TAATACAATA TATTCAGATA 
TATAGTCTAA CAGTCTCAAA AATAAGCTTT ATTGGACATT CTTTGGGCAA TTTAATAATC 
CGTTCAGTGC TTACAAGACC ACGGTTTAAA TATTACCTCA GCAGGCTTCA TACCTTTCTT 
TCACTTTCTG GACCTCACCT TGGTACACTC TACAATAGCA GTGCTCTTGT TAATACAGGT 
CTGTGGTTTA TGCAGAAATG GAAAAAATCA GGTTCTCTTT TACAATTGAC ATGTCGTGAT 
CATTCAGACC CTCGCCAAAC TTTCTTATAC AAGCTTAGTA ATAAAGCAGG GCTTCACTAC 
TTCAGAAATG TTGTCCTAGT GGGATCTCTG CAAGATCGCT ATGTTCCTTA TCATTCTGCC 
CGCATTGAAA TGTGCAAGAC AGCTTTGAAG GACAAACAGT CAGGACAGAT CTATTCAGAA 
ATGATCCACA ACTTACTTCG CCCGGTTCTG CAAAGCAAGG ACTGTAACTT GGTTCGATAT 
AATGTCATCA ATGCATTGCC CAATACAGCT GATTCACTCA TTGGCAGAGC TGCACATATA 
GCTGTTCTGG ATTCAGAAAT ATTTTTAGAG AAATTCTTTC TGGTTGCTGC CCTCAAATAT 
TTCCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FAM135A ( XM_025294945.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025150730.1 Bubalus bubalis family with sequence similarity 135 member A (FAM135A), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBu09039
Clone ID Related Accession (Same CDS sequence)	XM_006051060.1 , XM_025294945.1
Accession Version	XM_025294945.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	protein FAM135A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037554.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT 
CCGCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT 
TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG 
GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT 
GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA 
GATTATTCGG CAGATGATCT GAACGCCTTG CAGCTAATAA GCAGCCGCAC GCTGAAGCTG 
CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGCTAACG TCATGTTTGA TTATTTCCAC 
CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA 
AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA 
GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA 
TCACCAGATG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC 
CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT 
ACGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGAGGAAAT GGATGTAGAA 
GCTCGACTTA CTGAATTATG TGAAGAAGTT AAGAAAATAG AAAATCCTGA TGAACTGGCA 
GAACTTATAA ATATGAATCT TGCACAACTT TGCTCACTTT TAATGGCTTT ATGGGGACAA 
TTTCTGGAAG TTATAACACT ACATGAGGAA CTAAGACTAT TACTAGCACA AGAGCACCAT 
ACTCTGAGGG TGCGCAGGTT TTCTGAGGCC TTCTTTTGTT TTGAACATCC AAGAGAAGCT 
GCCATTGCAT ACCAGGAACT TCATGCTCAG AGTCATCTCC AGATGTGCAC CGCTATCAAA 
AACACTTCCT TCTGCAGCTC TCTCCCACCC CTGCCTATTG AATGTAGTGA ATTAGATGGA 
GATCTCAATT CGTTACCTAT TATCTTTGAA GATAGGTATT TAGATTCAGT TACTGAAGGT 
AAACTTGGTA TCTCCCAGGA TGATAGTGAA ATTGCACAAG TGGAACACAG TGTGGCATCC 
AGAAGCTCAT CAGACGATTG CCATGATCGT CAGTCTGCCC CCACTTCAGG AGTTAGGACA 
ATTGAAGTTA AGCCATGTAA TAAAGACTGT TTCAGTGGAG AGAAAGTAAC TGTTAAAATA 
GGACCTTGGA CAGAGCTTCA ACAAGATGAA ATATTTGTGG ATAATTTACA ACTGCCCAAC 
TTTGAGTCCT TAGACTCTAA TGGTAAATCT AAACCTATGG AAATAACACT TGAAAAGGAA 
GCTTTGCAGG AAGCTAAGTG TCATTCTGTC GGGGAATCGT TAGCTAAGTT AAGAAATAAT 
CAACCTGCTT CTACAAGAGA ATACCACCTT GTAGTGAGTG GAGACACCAT TAAGTTACCA 
GACATTAGTG CCACGTGTGC CTCATCTCGG TTCTCAGACT CAGGTGTGGA AAGTGAGCCA 
AGCTCTGTTG CAGCACATCC AAACCCTGAC ATAGTCTTTG AAACTGTACC AGGGCAAGGT 
CTTTTCATCA GTGAAAGGTT ATTTCCTCAG CTTTTGATGA AGCCTGACTG TAATGTAAAG 
TTTTCACTAG GCAGTCACTG TACTGAGAGT ACAAGTGCTT TCAGTGAGAT CCAGTCGTCC 
TTAACCTCCA TAAATTCTCT GCCTTCTGAT GACGAGCTGT CACCTGATGA AAATCCTAAG 
AAATCTGCTG TATCTGAATG CCACCTAAGT GATAGCAAAA CTGTGTTAAA TCTAGGAACA 
ACTGATATGC CAAAATGTGA CGATAGGAAA AAGTCAAGTA TTATTTTGCA GCAGCAGAGT 
GTCATATTTT CAGGGCATTC GGACAATGAA ACTATAGAAA TACATTCCTT AAATTCAAGC 
ACTAAAGACC CTTTACAATT TGTTTTTTCA GATGAAGATA CTTCTAGTGA TGTGAAAAGT 
AGTTGCAGCT CCAAACCTAA CTTGGACACT GTGTGTAAAG ACTCCCAGAG TCCTGATAAA 
TCCAGTGACT CTGTAGGAAC CACAGTTCCG TTAAGTTCAG AACTGGCTTG TTCAGGCACC 
CCTTGTGTCG TTTCAGGTTC CGTTTCCACC AGGGCAGATA GCAGTGAAGA TAGAACTGTG 
AAAAGAAAAA ACAGTGATGC ATTAAATCCC AAACAAATGT GTTCAGAAGC CCCTGCAGTT 
ACAGATGAAA CTTACCTGGG TACAGGTGAT GCTTTTTCAG CCAGTCCTGA TATGGTAAAG 
CAAGGGCTTG TGGAAAATTA TTTTGGCTGT CAAAGCAGTA CGGATGTTTC TGACACATGG 
GCTATTAGCT ACAGCCATTC AGTTAACCCT CAGAAAGAAA CTTCTGGGAA AGAAATGAGT 
AGTCCTCAGC GGGATCAGGG TAAAGATGAG GAAGAGGAAG AGCAGGATCA ACAAATGGTT 
CAAAACGGGT ATTATGAGGA GGCAGATTAC TCCTCTCTGG AGGCCGCGGG AGATGCTCAC 
TGTGCAGACG GAGCTGACCT GGGAGGAGAA AGACTTGCCA AGTCTGAAAG ACTGGCCGGC 
GACTATCTGA GCGATGGCAT AACCATGCCT ACTGTCTGTA CTTCTGGCTG TCTGTCCTTC 
CCGTCTGCAC CACGAGAGTC CCCTTGTGGC GTTAAGTACT CTTCTAAGAG TAAATGTGAC 
GCTGTTACCA AGCAGCCGAG CAGCGCCTCC CACAGCTGCA CCTCATCGCT GTCCTGGTAC 
GAAAACTCGC CAAAGCCTCA GATCCAAGCC TTCCTTCAGG CAAAAGATGA ATTGAAGCAA 
CTTAAACTCC CCGGGTTCAT GTACAGCGAT GTTCCTCTGC TGGCATCCTC AGTCCCTTAT 
TTTTGCGTGG AGGAGGAGGA GGAGGACCAT TATGAAAATG GAGTGCATCT CATTGTCTGT 
GTGCACGGCC TAGACGGTAA CAGTGCAGAT CTCCGATTAG TAAAAACTTA CATTGAACTT 
GGGCTGCCTG GGGAAAGAAT TGATTTTCTT ATGTCTGAAA GAAATCAGAA TGATACTTTT 
GCTGATTTTG ATAGCATGAC TGATCGCCTT TTGGATGAGA TAATACAATA TATTCAGATA 
TATAGTCTAA CAGTCTCAAA AATAAGCTTT ATTGGACATT CTTTGGGCAA TTTAATAATC 
CGTTCAGTGC TTACAAGACC ACGGTTTAAA TATTACCTCA GCAGGCTTCA TACCTTTCTT 
TCACTTTCTG GACCTCACCT TGGTACACTC TACAATAGCA GTGCTCTTGT TAATACAGGT 
CTGTGGTTTA TGCAGAAATG GAAAAAATCA GGTTCTCTTT TACAATTGAC ATGTCGTGAT 
CATTCAGACC CTCGCCAAAC TTTCTTATAC AAGCTTAGTA ATAAAGCAGG GCTTCACTAC 
TTCAGAAATG TTGTCCTAGT GGGATCTCTG CAAGATCGCT ATGTTCCTTA TCATTCTGCC 
CGCATTGAAA TGTGCAAGAC AGCTTTGAAG GACAAACAGT CAGGACAGAT CTATTCAGAA 
ATGATCCACA ACTTACTTCG CCCGGTTCTG CAAAGCAAGG ACTGTAACTT GGTTCGATAT 
AATGTCATCA ATGCATTGCC CAATACAGCT GATTCACTCA TTGGCAGAGC TGCACATATA 
GCTGTTCTGG ATTCAGAAAT ATTTTTAGAG AAATTCTTTC TGGTTGCTGC CCTCAAATAT 
TTCCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025150730.1
CDS	375..4343
Translation	MTEVQAMVEFSVELNKFYNVDLFQRGFYQIRASMKIPPRVPHRVEASLLHAAGMTLAFPASVHDSLICSKTFQILYKNEEVVLNDVMIFKVKMLLDEKKIEETLEEMNFLLSLDLHFTDGDYSADDLNALQLISSRTLKLHFSLQRGLHHHANVMFDYFHLSVVSVTVHASLVALHQPLISFPRPVKTTWLNRNAPAQNKDSVIPTLESVVFGINYTKQLSPDGCSFVIADSFLRHAYRFHYTLCAALLLAFKGLHSYFITVTEELPSCQKLELEEMDVEARLTELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVITLHEELRLLLAQEHHTLRVRRFSEAFFCFEHPREAAIAYQELHAQSHLQMCTAIKNTSFCSSLPPLPIECSELDGDLNSLPIIFEDRYLDSVTEGKLGISQDDSEIAQVEHSVASRSSSDDCHDRQSAPTSGVRTIEVKPCNKDCFSGEKVTVKIGPWTELQQDEIFVDNLQLPNFESLDSNGKSKPMEITLEKEALQEAKCHSVGESLAKLRNNQPASTREYHLVVSGDTIKLPDISATCASSRFSDSGVESEPSSVAAHPNPDIVFETVPGQGLFISERLFPQLLMKPDCNVKFSLGSHCTESTSAFSEIQSSLTSINSLPSDDELSPDENPKKSAVSECHLSDSKTVLNLGTTDMPKCDDRKKSSIILQQQSVIFSGHSDNETIEIHSLNSSTKDPLQFVFSDEDTSSDVKSSCSSKPNLDTVCKDSQSPDKSSDSVGTTVPLSSELACSGTPCVVSGSVSTRADSSEDRTVKRKNSDALNPKQMCSEAPAVTDETYLGTGDAFSASPDMVKQGLVENYFGCQSSTDVSDTWAISYSHSVNPQKETSGKEMSSPQRDQGKDEEEEEQDQQMVQNGYYEEADYSSLEAAGDAHCADGADLGGERLAKSERLAGDYLSDGITMPTVCTSGCLSFPSAPRESPCGVKYSSKSKCDAVTKQPSSASHSCTSSLSWYENSPKPQIQAFLQAKDELKQLKLPGFMYSDVPLLASSVPYFCVEEEEEDHYENGVHLIVCVHGLDGNSADLRLVKTYIELGLPGERIDFLMSERNQNDTFADFDSMTDRLLDEIIQYIQIYSLTVSKISFIGHSLGNLIIRSVLTRPRFKYYLSRLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSNKAGLHYFRNVVLVGSLQDRYVPYHSARIEMCKTALKDKQSGQIYSEMIHNLLRPVLQSKDCNLVRYNVINALPNTADSLIGRAAHIAVLDSEIFLEKFFLVAALKYFQ

Target ORF information:

RefSeq Version	XM_025294945.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis family with sequence similarity 135 member A (FAM135A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025294945.1

ORF Insert Sequence:

ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT 
CCGCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT 
TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG 
GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT 
GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA 
GATTATTCGG CAGATGATCT GAACGCCTTG CAGCTAATAA GCAGCCGCAC GCTGAAGCTG 
CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGCTAACG TCATGTTTGA TTATTTCCAC 
CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA 
AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA 
GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA 
TCACCAGATG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC 
CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT 
ACGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGAGGAAAT GGATGTAGAA 
GCTCGACTTA CTGAATTATG TGAAGAAGTT AAGAAAATAG AAAATCCTGA TGAACTGGCA 
GAACTTATAA ATATGAATCT TGCACAACTT TGCTCACTTT TAATGGCTTT ATGGGGACAA 
TTTCTGGAAG TTATAACACT ACATGAGGAA CTAAGACTAT TACTAGCACA AGAGCACCAT 
ACTCTGAGGG TGCGCAGGTT TTCTGAGGCC TTCTTTTGTT TTGAACATCC AAGAGAAGCT 
GCCATTGCAT ACCAGGAACT TCATGCTCAG AGTCATCTCC AGATGTGCAC CGCTATCAAA 
AACACTTCCT TCTGCAGCTC TCTCCCACCC CTGCCTATTG AATGTAGTGA ATTAGATGGA 
GATCTCAATT CGTTACCTAT TATCTTTGAA GATAGGTATT TAGATTCAGT TACTGAAGGT 
AAACTTGGTA TCTCCCAGGA TGATAGTGAA ATTGCACAAG TGGAACACAG TGTGGCATCC 
AGAAGCTCAT CAGACGATTG CCATGATCGT CAGTCTGCCC CCACTTCAGG AGTTAGGACA 
ATTGAAGTTA AGCCATGTAA TAAAGACTGT TTCAGTGGAG AGAAAGTAAC TGTTAAAATA 
GGACCTTGGA CAGAGCTTCA ACAAGATGAA ATATTTGTGG ATAATTTACA ACTGCCCAAC 
TTTGAGTCCT TAGACTCTAA TGGTAAATCT AAACCTATGG AAATAACACT TGAAAAGGAA 
GCTTTGCAGG AAGCTAAGTG TCATTCTGTC GGGGAATCGT TAGCTAAGTT AAGAAATAAT 
CAACCTGCTT CTACAAGAGA ATACCACCTT GTAGTGAGTG GAGACACCAT TAAGTTACCA 
GACATTAGTG CCACGTGTGC CTCATCTCGG TTCTCAGACT CAGGTGTGGA AAGTGAGCCA 
AGCTCTGTTG CAGCACATCC AAACCCTGAC ATAGTCTTTG AAACTGTACC AGGGCAAGGT 
CTTTTCATCA GTGAAAGGTT ATTTCCTCAG CTTTTGATGA AGCCTGACTG TAATGTAAAG 
TTTTCACTAG GCAGTCACTG TACTGAGAGT ACAAGTGCTT TCAGTGAGAT CCAGTCGTCC 
TTAACCTCCA TAAATTCTCT GCCTTCTGAT GACGAGCTGT CACCTGATGA AAATCCTAAG 
AAATCTGCTG TATCTGAATG CCACCTAAGT GATAGCAAAA CTGTGTTAAA TCTAGGAACA 
ACTGATATGC CAAAATGTGA CGATAGGAAA AAGTCAAGTA TTATTTTGCA GCAGCAGAGT 
GTCATATTTT CAGGGCATTC GGACAATGAA ACTATAGAAA TACATTCCTT AAATTCAAGC 
ACTAAAGACC CTTTACAATT TGTTTTTTCA GATGAAGATA CTTCTAGTGA TGTGAAAAGT 
AGTTGCAGCT CCAAACCTAA CTTGGACACT GTGTGTAAAG ACTCCCAGAG TCCTGATAAA 
TCCAGTGACT CTGTAGGAAC CACAGTTCCG TTAAGTTCAG AACTGGCTTG TTCAGGCACC 
CCTTGTGTCG TTTCAGGTTC CGTTTCCACC AGGGCAGATA GCAGTGAAGA TAGAACTGTG 
AAAAGAAAAA ACAGTGATGC ATTAAATCCC AAACAAATGT GTTCAGAAGC CCCTGCAGTT 
ACAGATGAAA CTTACCTGGG TACAGGTGAT GCTTTTTCAG CCAGTCCTGA TATGGTAAAG 
CAAGGGCTTG TGGAAAATTA TTTTGGCTGT CAAAGCAGTA CGGATGTTTC TGACACATGG 
GCTATTAGCT ACAGCCATTC AGTTAACCCT CAGAAAGAAA CTTCTGGGAA AGAAATGAGT 
AGTCCTCAGC GGGATCAGGG TAAAGATGAG GAAGAGGAAG AGCAGGATCA ACAAATGGTT 
CAAAACGGGT ATTATGAGGA GGCAGATTAC TCCTCTCTGG AGGCCGCGGG AGATGCTCAC 
TGTGCAGACG GAGCTGACCT GGGAGGAGAA AGACTTGCCA AGTCTGAAAG ACTGGCCGGC 
GACTATCTGA GCGATGGCAT AACCATGCCT ACTGTCTGTA CTTCTGGCTG TCTGTCCTTC 
CCGTCTGCAC CACGAGAGTC CCCTTGTGGC GTTAAGTACT CTTCTAAGAG TAAATGTGAC 
GCTGTTACCA AGCAGCCGAG CAGCGCCTCC CACAGCTGCA CCTCATCGCT GTCCTGGTAC 
GAAAACTCGC CAAAGCCTCA GATCCAAGCC TTCCTTCAGG CAAAAGATGA ATTGAAGCAA 
CTTAAACTCC CCGGGTTCAT GTACAGCGAT GTTCCTCTGC TGGCATCCTC AGTCCCTTAT 
TTTTGCGTGG AGGAGGAGGA GGAGGACCAT TATGAAAATG GAGTGCATCT CATTGTCTGT 
GTGCACGGCC TAGACGGTAA CAGTGCAGAT CTCCGATTAG TAAAAACTTA CATTGAACTT 
GGGCTGCCTG GGGAAAGAAT TGATTTTCTT ATGTCTGAAA GAAATCAGAA TGATACTTTT 
GCTGATTTTG ATAGCATGAC TGATCGCCTT TTGGATGAGA TAATACAATA TATTCAGATA 
TATAGTCTAA CAGTCTCAAA AATAAGCTTT ATTGGACATT CTTTGGGCAA TTTAATAATC 
CGTTCAGTGC TTACAAGACC ACGGTTTAAA TATTACCTCA GCAGGCTTCA TACCTTTCTT 
TCACTTTCTG GACCTCACCT TGGTACACTC TACAATAGCA GTGCTCTTGT TAATACAGGT 
CTGTGGTTTA TGCAGAAATG GAAAAAATCA GGTTCTCTTT TACAATTGAC ATGTCGTGAT 
CATTCAGACC CTCGCCAAAC TTTCTTATAC AAGCTTAGTA ATAAAGCAGG GCTTCACTAC 
TTCAGAAATG TTGTCCTAGT GGGATCTCTG CAAGATCGCT ATGTTCCTTA TCATTCTGCC 
CGCATTGAAA TGTGCAAGAC AGCTTTGAAG GACAAACAGT CAGGACAGAT CTATTCAGAA 
ATGATCCACA ACTTACTTCG CCCGGTTCTG CAAAGCAAGG ACTGTAACTT GGTTCGATAT 
AATGTCATCA ATGCATTGCC CAATACAGCT GATTCACTCA TTGGCAGAGC TGCACATATA 
GCTGTTCTGG ATTCAGAAAT ATTTTTAGAG AAATTCTTTC TGGTTGCTGC CCTCAAATAT 
TTCCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FAM135A ( XM_025294946.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025150731.1 Bubalus bubalis family with sequence similarity 135 member A (FAM135A), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBu44226
Clone ID Related Accession (Same CDS sequence)	XM_025294946.1
Accession Version	XM_025294946.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	protein FAM135A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037554.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGTCC TTTATGAGCG CCTTCTCAGA AGAAAACAGC CAGCAACCCA GAAAGACACC 
TGTCTCGAGG AAATGGATGT AGAAGCTCGA CTTACTGAAT TATGTGAAGA AGTTAAGAAA 
ATAGAAAATC CTGATGAACT GGCAGAACTT ATAAATATGA ATCTTGCACA ACTTTGCTCA 
CTTTTAATGG CTTTATGGGG ACAATTTCTG GAAGTTATAA CACTACATGA GGAACTAAGA 
CTATTACTAG CACAAGAGCA CCATACTCTG AGGGTGCGCA GGTTTTCTGA GGCCTTCTTT 
TGTTTTGAAC ATCCAAGAGA AGCTGCCATT GCATACCAGG AACTTCATGC TCAGAGTCAT 
CTCCAGATGT GCACCGCTAT CAAAAACACT TCCTTCTGCA GCTCTCTCCC ACCCCTGCCT 
ATTGAATGTA GTGAATTAGA TGGAGATCTC AATTCGTTAC CTATTATCTT TGAAGATAGG 
TATTTAGATT CAGTTACTGA AGACTTAGAT GCACCCTGGA TGGGAATTCA GAGTCTTCAG 
AGATCAGAGT CCAGTAGAAT GGATAAATGT GAGACTGAAG AAAGCACTCT AGCTGGACTT 
TCCAGCCCAG AGTTGAAAAT CAGACCTGCT GGGACCTCCA GTTTTTGGTC TACAGAAGGT 
GAAAAGCAGC TAACAAAGTC TCTGAAAGGA AAGAATGAAG AATCAAATAA ATCCAAGGTT 
AAGGTTACTA AGCTCATGAA AACAATGAAA CCTGAAAACA CAAAAAAATT AATAAAACAG 
AACTCTAAGG ATTTGGTGGT TTTGGTAGGC TACAAATGCT TGAAAAGTAC AGCATCAAAT 
GATCTCTTTA AAAGCTTTGA AGGCAACCCT ACACATAGTC AGAAGGAAGG TCTGGACCCC 
ACAATATATG GATATAATTT TGATCCAAAG ACCTACATCA GACAGACAAG TCAAAAGGAA 
GCTAGCTATT TGCCAGCTAA TACAGAAAGA ACTGAACAAA AGTCTCCAGA CATTGAAAAT 
ATGCAACCAG ACCTGTTTGA TCCTTTGAAC TCTGGCAGCC TAAATCTTTG TGCAAATTTG 
TCCATTTCAG GTAAACTTGG TATCTCCCAG GATGATAGTG AAATTGCACA AGTGGAACAC 
AGTGTGGCAT CCAGAAGCTC ATCAGACGAT TGCCATGATC GTCAGTCTGC CCCCACTTCA 
GGAGTTAGGA CAATTGAAGT TAAGCCATGT AATAAAGACT GTTTCAGTGG AGAGAAAGTA 
ACTGTTAAAA TAGGACCTTG GACAGAGCTT CAACAAGATG AAATATTTGT GGATAATTTA 
CAACTGCCCA ACTTTGAGTC CTTAGACTCT AATGGTAAAT CTAAACCTAT GGAAATAACA 
CTTGAAAAGG AAGCTTTGCA GGAAGCTAAG TGTCATTCTG TCGGGGAATC GTTAGCTAAG 
TTAAGAAATA ATCAACCTGC TTCTACAAGA GAATACCACC TTGTAGTGAG TGGAGACACC 
ATTAAGTTAC CAGACATTAG TGCCACGTGT GCCTCATCTC GGTTCTCAGA CTCAGGTGTG 
GAAAGTGAGC CAAGCTCTGT TGCAGCACAT CCAAACCCTG ACATAGTCTT TGAAACTGTA 
CCAGGGCAAG GTCTTTTCAT CAGTGAAAGG TTATTTCCTC AGCTTTTGAT GAAGCCTGAC 
TGTAATGTAA AGTTTTCACT AGGCAGTCAC TGTACTGAGA GTACAAGTGC TTTCAGTGAG 
ATCCAGTCGT CCTTAACCTC CATAAATTCT CTGCCTTCTG ATGACGAGCT GTCACCTGAT 
GAAAATCCTA AGAAATCTGC TGTATCTGAA TGCCACCTAA GTGATAGCAA AACTGTGTTA 
AATCTAGGAA CAACTGATAT GCCAAAATGT GACGATAGGA AAAAGTCAAG TATTATTTTG 
CAGCAGCAGA GTGTCATATT TTCAGGGCAT TCGGACAATG AAACTATAGA AATACATTCC 
TTAAATTCAA GCACTAAAGA CCCTTTACAA TTTGTTTTTT CAGATGAAGA TACTTCTAGT 
GATGTGAAAA GTAGTTGCAG CTCCAAACCT AACTTGGACA CTGTGTGTAA AGACTCCCAG 
AGTCCTGATA AATCCAGTGA CTCTGTAGGA ACCACAGTTC CGTTAAGTTC AGAACTGGCT 
TGTTCAGGCA CCCCTTGTGT CGTTTCAGGT TCCGTTTCCA CCAGGGCAGA TAGCAGTGAA 
GATAGAACTG TGAAAAGAAA AAACAGTGAT GCATTAAATC CCAAACAAAT GTGTTCAGAA 
GCCCCTGCAG TTACAGATGA AACTTACCTG GGTACAGGTG ATGCTTTTTC AGCCAGTCCT 
GATATGGTAA AGCAAGGGCT TGTGGAAAAT TATTTTGGCT GTCAAAGCAG TACGGATGTT 
TCTGACACAT GGGCTATTAG CTACAGCCAT TCAGTTAACC CTCAGAAAGA AACTTCTGGG 
AAAGAAATGA GTAGTCCTCA GCGGGATCAG GGTAAAGATG AGGAAGAGGA AGAGCAGGAT 
CAACAAATGG TTCAAAACGG GTATTATGAG GAGGCAGATT ACTCCTCTCT GGAGGCCGCG 
GGAGATGCTC ACTGTGCAGA CGGAGCTGAC CTGGGAGGAG AAAGACTTGC CAAGTCTGAA 
AGACTGGCCG GCGACTATCT GAGCGATGGC ATAACCATGC CTACTGTCTG TACTTCTGGC 
TGTCTGTCCT TCCCGTCTGC ACCACGAGAG TCCCCTTGTG GCGTTAAGTA CTCTTCTAAG 
AGTAAATGTG ACGCTGTTAC CAAGCAGCCG AGCAGCGCCT CCCACAGCTG CACCTCATCG 
CTGTCCTGGT ACGAAAACTC GCCAAAGCCT CAGATCCAAG CCTTCCTTCA GGCAAAAGAT 
GAATTGAAGC AACTTAAACT CCCCGGGTTC ATGTACAGCG ATGTTCCTCT GCTGGCATCC 
TCAGTCCCTT ATTTTTGCGT GGAGGAGGAG GAGGAGGACC ATTATGAAAA TGGAGTGCAT 
CTCATTGTCT GTGTGCACGG CCTAGACGGT AACAGTGCAG ATCTCCGATT AGTAAAAACT 
TACATTGAAC TTGGGCTGCC TGGGGAAAGA ATTGATTTTC TTATGTCTGA AAGAAATCAG 
AATGATACTT TTGCTGATTT TGATAGCATG ACTGATCGCC TTTTGGATGA GATAATACAA 
TATATTCAGA TATATAGTCT AACAGTCTCA AAAATAAGCT TTATTGGACA TTCTTTGGGC 
AATTTAATAA TCCGTTCAGT GCTTACAAGA CCACGGTTTA AATATTACCT CAGCAGGCTT 
CATACCTTTC TTTCACTTTC TGGACCTCAC CTTGGTACAC TCTACAATAG CAGTGCTCTT 
GTTAATACAG GTCTGTGGTT TATGCAGAAA TGGAAAAAAT CAGGTTCTCT TTTACAATTG 
ACATGTCGTG ATCATTCAGA CCCTCGCCAA ACTTTCTTAT ACAAGCTTAG TAATAAAGCA 
GGGCTTCACT ACTTCAGAAA TGTTGTCCTA GTGGGATCTC TGCAAGATCG CTATGTTCCT 
TATCATTCTG CCCGCATTGA AATGTGCAAG ACAGCTTTGA AGGACAAACA GTCAGGACAG 
ATCTATTCAG AAATGATCCA CAACTTACTT CGCCCGGTTC TGCAAAGCAA GGACTGTAAC 
TTGGTTCGAT ATAATGTCAT CAATGCATTG CCCAATACAG CTGATTCACT CATTGGCAGA 
GCTGCACATA TAGCTGTTCT GGATTCAGAA ATATTTTTAG AGAAATTCTT TCTGGTTGCT 
GCCCTCAAAT ATTTCCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025150731.1
CDS	120..3920
Translation	MQVLYERLLRRKQPATQKDTCLEEMDVEARLTELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVITLHEELRLLLAQEHHTLRVRRFSEAFFCFEHPREAAIAYQELHAQSHLQMCTAIKNTSFCSSLPPLPIECSELDGDLNSLPIIFEDRYLDSVTEDLDAPWMGIQSLQRSESSRMDKCETEESTLAGLSSPELKIRPAGTSSFWSTEGEKQLTKSLKGKNEESNKSKVKVTKLMKTMKPENTKKLIKQNSKDLVVLVGYKCLKSTASNDLFKSFEGNPTHSQKEGLDPTIYGYNFDPKTYIRQTSQKEASYLPANTERTEQKSPDIENMQPDLFDPLNSGSLNLCANLSISGKLGISQDDSEIAQVEHSVASRSSSDDCHDRQSAPTSGVRTIEVKPCNKDCFSGEKVTVKIGPWTELQQDEIFVDNLQLPNFESLDSNGKSKPMEITLEKEALQEAKCHSVGESLAKLRNNQPASTREYHLVVSGDTIKLPDISATCASSRFSDSGVESEPSSVAAHPNPDIVFETVPGQGLFISERLFPQLLMKPDCNVKFSLGSHCTESTSAFSEIQSSLTSINSLPSDDELSPDENPKKSAVSECHLSDSKTVLNLGTTDMPKCDDRKKSSIILQQQSVIFSGHSDNETIEIHSLNSSTKDPLQFVFSDEDTSSDVKSSCSSKPNLDTVCKDSQSPDKSSDSVGTTVPLSSELACSGTPCVVSGSVSTRADSSEDRTVKRKNSDALNPKQMCSEAPAVTDETYLGTGDAFSASPDMVKQGLVENYFGCQSSTDVSDTWAISYSHSVNPQKETSGKEMSSPQRDQGKDEEEEEQDQQMVQNGYYEEADYSSLEAAGDAHCADGADLGGERLAKSERLAGDYLSDGITMPTVCTSGCLSFPSAPRESPCGVKYSSKSKCDAVTKQPSSASHSCTSSLSWYENSPKPQIQAFLQAKDELKQLKLPGFMYSDVPLLASSVPYFCVEEEEEDHYENGVHLIVCVHGLDGNSADLRLVKTYIELGLPGERIDFLMSERNQNDTFADFDSMTDRLLDEIIQYIQIYSLTVSKISFIGHSLGNLIIRSVLTRPRFKYYLSRLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSNKAGLHYFRNVVLVGSLQDRYVPYHSARIEMCKTALKDKQSGQIYSEMIHNLLRPVLQSKDCNLVRYNVINALPNTADSLIGRAAHIAVLDSEIFLEKFFLVAALKYFQ

Target ORF information:

RefSeq Version	XM_025294946.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis family with sequence similarity 135 member A (FAM135A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025294946.1

ORF Insert Sequence:

ATGCAGGTCC TTTATGAGCG CCTTCTCAGA AGAAAACAGC CAGCAACCCA GAAAGACACC 
TGTCTCGAGG AAATGGATGT AGAAGCTCGA CTTACTGAAT TATGTGAAGA AGTTAAGAAA 
ATAGAAAATC CTGATGAACT GGCAGAACTT ATAAATATGA ATCTTGCACA ACTTTGCTCA 
CTTTTAATGG CTTTATGGGG ACAATTTCTG GAAGTTATAA CACTACATGA GGAACTAAGA 
CTATTACTAG CACAAGAGCA CCATACTCTG AGGGTGCGCA GGTTTTCTGA GGCCTTCTTT 
TGTTTTGAAC ATCCAAGAGA AGCTGCCATT GCATACCAGG AACTTCATGC TCAGAGTCAT 
CTCCAGATGT GCACCGCTAT CAAAAACACT TCCTTCTGCA GCTCTCTCCC ACCCCTGCCT 
ATTGAATGTA GTGAATTAGA TGGAGATCTC AATTCGTTAC CTATTATCTT TGAAGATAGG 
TATTTAGATT CAGTTACTGA AGACTTAGAT GCACCCTGGA TGGGAATTCA GAGTCTTCAG 
AGATCAGAGT CCAGTAGAAT GGATAAATGT GAGACTGAAG AAAGCACTCT AGCTGGACTT 
TCCAGCCCAG AGTTGAAAAT CAGACCTGCT GGGACCTCCA GTTTTTGGTC TACAGAAGGT 
GAAAAGCAGC TAACAAAGTC TCTGAAAGGA AAGAATGAAG AATCAAATAA ATCCAAGGTT 
AAGGTTACTA AGCTCATGAA AACAATGAAA CCTGAAAACA CAAAAAAATT AATAAAACAG 
AACTCTAAGG ATTTGGTGGT TTTGGTAGGC TACAAATGCT TGAAAAGTAC AGCATCAAAT 
GATCTCTTTA AAAGCTTTGA AGGCAACCCT ACACATAGTC AGAAGGAAGG TCTGGACCCC 
ACAATATATG GATATAATTT TGATCCAAAG ACCTACATCA GACAGACAAG TCAAAAGGAA 
GCTAGCTATT TGCCAGCTAA TACAGAAAGA ACTGAACAAA AGTCTCCAGA CATTGAAAAT 
ATGCAACCAG ACCTGTTTGA TCCTTTGAAC TCTGGCAGCC TAAATCTTTG TGCAAATTTG 
TCCATTTCAG GTAAACTTGG TATCTCCCAG GATGATAGTG AAATTGCACA AGTGGAACAC 
AGTGTGGCAT CCAGAAGCTC ATCAGACGAT TGCCATGATC GTCAGTCTGC CCCCACTTCA 
GGAGTTAGGA CAATTGAAGT TAAGCCATGT AATAAAGACT GTTTCAGTGG AGAGAAAGTA 
ACTGTTAAAA TAGGACCTTG GACAGAGCTT CAACAAGATG AAATATTTGT GGATAATTTA 
CAACTGCCCA ACTTTGAGTC CTTAGACTCT AATGGTAAAT CTAAACCTAT GGAAATAACA 
CTTGAAAAGG AAGCTTTGCA GGAAGCTAAG TGTCATTCTG TCGGGGAATC GTTAGCTAAG 
TTAAGAAATA ATCAACCTGC TTCTACAAGA GAATACCACC TTGTAGTGAG TGGAGACACC 
ATTAAGTTAC CAGACATTAG TGCCACGTGT GCCTCATCTC GGTTCTCAGA CTCAGGTGTG 
GAAAGTGAGC CAAGCTCTGT TGCAGCACAT CCAAACCCTG ACATAGTCTT TGAAACTGTA 
CCAGGGCAAG GTCTTTTCAT CAGTGAAAGG TTATTTCCTC AGCTTTTGAT GAAGCCTGAC 
TGTAATGTAA AGTTTTCACT AGGCAGTCAC TGTACTGAGA GTACAAGTGC TTTCAGTGAG 
ATCCAGTCGT CCTTAACCTC CATAAATTCT CTGCCTTCTG ATGACGAGCT GTCACCTGAT 
GAAAATCCTA AGAAATCTGC TGTATCTGAA TGCCACCTAA GTGATAGCAA AACTGTGTTA 
AATCTAGGAA CAACTGATAT GCCAAAATGT GACGATAGGA AAAAGTCAAG TATTATTTTG 
CAGCAGCAGA GTGTCATATT TTCAGGGCAT TCGGACAATG AAACTATAGA AATACATTCC 
TTAAATTCAA GCACTAAAGA CCCTTTACAA TTTGTTTTTT CAGATGAAGA TACTTCTAGT 
GATGTGAAAA GTAGTTGCAG CTCCAAACCT AACTTGGACA CTGTGTGTAA AGACTCCCAG 
AGTCCTGATA AATCCAGTGA CTCTGTAGGA ACCACAGTTC CGTTAAGTTC AGAACTGGCT 
TGTTCAGGCA CCCCTTGTGT CGTTTCAGGT TCCGTTTCCA CCAGGGCAGA TAGCAGTGAA 
GATAGAACTG TGAAAAGAAA AAACAGTGAT GCATTAAATC CCAAACAAAT GTGTTCAGAA 
GCCCCTGCAG TTACAGATGA AACTTACCTG GGTACAGGTG ATGCTTTTTC AGCCAGTCCT 
GATATGGTAA AGCAAGGGCT TGTGGAAAAT TATTTTGGCT GTCAAAGCAG TACGGATGTT 
TCTGACACAT GGGCTATTAG CTACAGCCAT TCAGTTAACC CTCAGAAAGA AACTTCTGGG 
AAAGAAATGA GTAGTCCTCA GCGGGATCAG GGTAAAGATG AGGAAGAGGA AGAGCAGGAT 
CAACAAATGG TTCAAAACGG GTATTATGAG GAGGCAGATT ACTCCTCTCT GGAGGCCGCG 
GGAGATGCTC ACTGTGCAGA CGGAGCTGAC CTGGGAGGAG AAAGACTTGC CAAGTCTGAA 
AGACTGGCCG GCGACTATCT GAGCGATGGC ATAACCATGC CTACTGTCTG TACTTCTGGC 
TGTCTGTCCT TCCCGTCTGC ACCACGAGAG TCCCCTTGTG GCGTTAAGTA CTCTTCTAAG 
AGTAAATGTG ACGCTGTTAC CAAGCAGCCG AGCAGCGCCT CCCACAGCTG CACCTCATCG 
CTGTCCTGGT ACGAAAACTC GCCAAAGCCT CAGATCCAAG CCTTCCTTCA GGCAAAAGAT 
GAATTGAAGC AACTTAAACT CCCCGGGTTC ATGTACAGCG ATGTTCCTCT GCTGGCATCC 
TCAGTCCCTT ATTTTTGCGT GGAGGAGGAG GAGGAGGACC ATTATGAAAA TGGAGTGCAT 
CTCATTGTCT GTGTGCACGG CCTAGACGGT AACAGTGCAG ATCTCCGATT AGTAAAAACT 
TACATTGAAC TTGGGCTGCC TGGGGAAAGA ATTGATTTTC TTATGTCTGA AAGAAATCAG 
AATGATACTT TTGCTGATTT TGATAGCATG ACTGATCGCC TTTTGGATGA GATAATACAA 
TATATTCAGA TATATAGTCT AACAGTCTCA AAAATAAGCT TTATTGGACA TTCTTTGGGC 
AATTTAATAA TCCGTTCAGT GCTTACAAGA CCACGGTTTA AATATTACCT CAGCAGGCTT 
CATACCTTTC TTTCACTTTC TGGACCTCAC CTTGGTACAC TCTACAATAG CAGTGCTCTT 
GTTAATACAG GTCTGTGGTT TATGCAGAAA TGGAAAAAAT CAGGTTCTCT TTTACAATTG 
ACATGTCGTG ATCATTCAGA CCCTCGCCAA ACTTTCTTAT ACAAGCTTAG TAATAAAGCA 
GGGCTTCACT ACTTCAGAAA TGTTGTCCTA GTGGGATCTC TGCAAGATCG CTATGTTCCT 
TATCATTCTG CCCGCATTGA AATGTGCAAG ACAGCTTTGA AGGACAAACA GTCAGGACAG 
ATCTATTCAG AAATGATCCA CAACTTACTT CGCCCGGTTC TGCAAAGCAA GGACTGTAAC 
TTGGTTCGAT ATAATGTCAT CAATGCATTG CCCAATACAG CTGATTCACT CATTGGCAGA 
GCTGCACATA TAGCTGTTCT GGATTCAGAA ATATTTTTAG AGAAATTCTT TCTGGTTGCT 
GCCCTCAAAT ATTTCCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FAM135A ( XM_025294942.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025150727.1 Bubalus bubalis family with sequence similarity 135 member A (FAM135A), transcript variant X2, mRNA.

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CloneID	OBu09036
Clone ID Related Accession (Same CDS sequence)	XM_025294942.1 , XM_006051057.1 , XM_025294941.1
Accession Version	XM_025294942.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	protein FAM135A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037554.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT 
CCGCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT 
TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG 
GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT 
GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA 
GATTATTCGG CAGATGATCT GAACGCCTTG CAGCTAATAA GCAGCCGCAC GCTGAAGCTG 
CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGCTAACG TCATGTTTGA TTATTTCCAC 
CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA 
AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA 
GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA 
TCACCAGATG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC 
CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT 
ACGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGCCAAAGC CAACATGCAG 
GTCCTTTATG AGCGCCTTCT CAGAAGAAAA CAGCCAGCAA CCCAGAAAGA CACCTGTCTC 
GAGGAAATGG ATGTAGAAGC TCGACTTACT GAATTATGTG AAGAAGTTAA GAAAATAGAA 
AATCCTGATG AACTGGCAGA ACTTATAAAT ATGAATCTTG CACAACTTTG CTCACTTTTA 
ATGGCTTTAT GGGGACAATT TCTGGAAGTT ATAACACTAC ATGAGGAACT AAGACTATTA 
CTAGCACAAG AGCACCATAC TCTGAGGGTG CGCAGGTTTT CTGAGGCCTT CTTTTGTTTT 
GAACATCCAA GAGAAGCTGC CATTGCATAC CAGGAACTTC ATGCTCAGAG TCATCTCCAG 
ATGTGCACCG CTATCAAAAA CACTTCCTTC TGCAGCTCTC TCCCACCCCT GCCTATTGAA 
TGTAGTGAAT TAGATGGAGA TCTCAATTCG TTACCTATTA TCTTTGAAGA TAGGTATTTA 
GATTCAGTTA CTGAAGACTT AGATGCACCC TGGATGGGAA TTCAGAGTCT TCAGAGATCA 
GAGTCCAGTA GAATGGATAA ATGTGAGACT GAAGAAAGCA CTCTAGCTGG ACTTTCCAGC 
CCAGAGTTGA AAATCAGACC TGCTGGGACC TCCAGTTTTT GGTCTACAGA AGGTGAAAAG 
CAGCTAACAA AGTCTCTGAA AGGAAAGAAT GAAGAATCAA ATAAATCCAA GGTTAAGGTT 
ACTAAGCTCA TGAAAACAAT GAAACCTGAA AACACAAAAA AATTAATAAA ACAGAACTCT 
AAGGATTTGG TGGTTTTGGT AGGCTACAAA TGCTTGAAAA GTACAGCATC AAATGATCTC 
TTTAAAAGCT TTGAAGGCAA CCCTACACAT AGTCAGAAGG AAGGTCTGGA CCCCACAATA 
TATGGATATA ATTTTGATCC AAAGACCTAC ATCAGACAGA CAAGTCAAAA GGAAGCTAGC 
TATTTGCCAG CTAATACAGA AAGAACTGAA CAAAAGTCTC CAGACATTGA AAATATGCAA 
CCAGACCTGT TTGATCCTTT GAACTCTGGC AGCCTAAATC TTTGTGCAAA TTTGTCCATT 
TCAGGTAAAC TTGGTATCTC CCAGGATGAT AGTGAAATTG CACAAGTGGA ACACAGTGTG 
GCATCCAGAA GCTCATCAGA CGATTGCCAT GATCGTCAGT CTGCCCCCAC TTCAGGAGTT 
AGGACAATTG AAGTTAAGCC ATGTAATAAA GACTGTTTCA GTGGAGAGAA AGTAACTGTT 
AAAATAGGAC CTTGGACAGA GCTTCAACAA GATGAAATAT TTGTGGATAA TTTACAACTG 
CCCAACTTTG AGTCCTTAGA CTCTAATGGT AAATCTAAAC CTATGGAAAT AACACTTGAA 
AAGGAAGCTT TGCAGGAAGC TAAGTGTCAT TCTGTCGGGG AATCGTTAGC TAAGTTAAGA 
AATAATCAAC CTGCTTCTAC AAGAGAATAC CACCTTGTAG TGAGTGGAGA CACCATTAAG 
TTACCAGACA TTAGTGCCAC GTGTGCCTCA TCTCGGTTCT CAGACTCAGG TGTGGAAAGT 
GAGCCAAGCT CTGTTGCAGC ACATCCAAAC CCTGACATAG TCTTTGAAAC TGTACCAGGG 
CAAGGTCTTT TCATCAGTGA AAGGTTATTT CCTCAGCTTT TGATGAAGCC TGACTGTAAT 
GTAAAGTTTT CACTAGGCAG TCACTGTACT GAGAGTACAA GTGCTTTCAG TGAGATCCAG 
TCGTCCTTAA CCTCCATAAA TTCTCTGCCT TCTGATGACG AGCTGTCACC TGATGAAAAT 
CCTAAGAAAT CTGCTGTATC TGAATGCCAC CTAAGTGATA GCAAAACTGT GTTAAATCTA 
GGAACAACTG ATATGCCAAA ATGTGACGAT AGGAAAAAGT CAAGTATTAT TTTGCAGCAG 
CAGAGTGTCA TATTTTCAGG GCATTCGGAC AATGAAACTA TAGAAATACA TTCCTTAAAT 
TCAAGCACTA AAGACCCTTT ACAATTTGTT TTTTCAGATG AAGATACTTC TAGTGATGTG 
AAAAGTAGTT GCAGCTCCAA ACCTAACTTG GACACTGTGT GTAAAGACTC CCAGAGTCCT 
GATAAATCCA GTGACTCTGT AGGAACCACA GTTCCGTTAA GTTCAGAACT GGCTTGTTCA 
GGCACCCCTT GTGTCGTTTC AGGTTCCGTT TCCACCAGGG CAGATAGCAG TGAAGATAGA 
ACTGTGAAAA GAAAAAACAG TGATGCATTA AATCCCAAAC AAATGTGTTC AGAAGCCCCT 
GCAGTTACAG ATGAAACTTA CCTGGGTACA GGTGATGCTT TTTCAGCCAG TCCTGATATG 
GTAAAGCAAG GGCTTGTGGA AAATTATTTT GGCTGTCAAA GCAGTACGGA TGTTTCTGAC 
ACATGGGCTA TTAGCTACAG CCATTCAGTT AACCCTCAGA AAGAAACTTC TGGGAAAGAA 
ATGAGTAGTC CTCAGCGGGA TCAGGGTAAA GATGAGGAAG AGGAAGAGCA GGATCAACAA 
ATGGTTCAAA ACGGGTATTA TGAGGAGGCA GATTACTCCT CTCTGGAGGC CGCGGGAGAT 
GCTCACTGTG CAGACGGAGC TGACCTGGGA GGAGAAAGAC TTGCCAAGTC TGAAAGACTG 
GCCGGCGACT ATCTGAGCGA TGGCATAACC ATGCCTACTG TCTGTACTTC TGGCTGTCTG 
TCCTTCCCGT CTGCACCACG AGAGTCCCCT TGTGGCGTTA AGTACTCTTC TAAGAGTAAA 
TGTGACGCTG TTACCAAGCA GCCGAGCAGC GCCTCCCACA GCTGCACCTC ATCGCTGTCC 
TGGTACGAAA ACTCGCCAAA GCCTCAGATC CAAGCCTTCC TTCAGGCAAA AGATGAATTG 
AAGCAACTTA AACTCCCCGG GTTCATGTAC AGCGATGTTC CTCTGCTGGC ATCCTCAGTC 
CCTTATTTTT GCGTGGAGGA GGAGGAGGAG GACCATTATG AAAATGGAGT GCATCTCATT 
GTCTGTGTGC ACGGCCTAGA CGGTAACAGT GCAGATCTCC GATTAGTAAA AACTTACATT 
GAACTTGGGC TGCCTGGGGA AAGAATTGAT TTTCTTATGT CTGAAAGAAA TCAGAATGAT 
ACTTTTGCTG ATTTTGATAG CATGACTGAT CGCCTTTTGG ATGAGATAAT ACAATATATT 
CAGATATATA GTCTAACAGT CTCAAAAATA AGCTTTATTG GACATTCTTT GGGCAATTTA 
ATAATCCGTT CAGTGCTTAC AAGACCACGG TTTAAATATT ACCTCAGCAG GCTTCATACC 
TTTCTTTCAC TTTCTGGACC TCACCTTGGT ACACTCTACA ATAGCAGTGC TCTTGTTAAT 
ACAGGTCTGT GGTTTATGCA GAAATGGAAA AAATCAGGTT CTCTTTTACA ATTGACATGT 
CGTGATCATT CAGACCCTCG CCAAACTTTC TTATACAAGC TTAGTAATAA AGCAGGGCTT 
CACTACTTCA GAAATGTTGT CCTAGTGGGA TCTCTGCAAG ATCGCTATGT TCCTTATCAT 
TCTGCCCGCA TTGAAATGTG CAAGACAGCT TTGAAGGACA AACAGTCAGG ACAGATCTAT 
TCAGAAATGA TCCACAACTT ACTTCGCCCG GTTCTGCAAA GCAAGGACTG TAACTTGGTT 
CGATATAATG TCATCAATGC ATTGCCCAAT ACAGCTGATT CACTCATTGG CAGAGCTGCA 
CATATAGCTG TTCTGGATTC AGAAATATTT TTAGAGAAAT TCTTTCTGGT TGCTGCCCTC 
AAATATTTCC AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025150727.1
CDS	269..4903
Translation	MTEVQAMVEFSVELNKFYNVDLFQRGFYQIRASMKIPPRVPHRVEASLLHAAGMTLAFPASVHDSLICSKTFQILYKNEEVVLNDVMIFKVKMLLDEKKIEETLEEMNFLLSLDLHFTDGDYSADDLNALQLISSRTLKLHFSLQRGLHHHANVMFDYFHLSVVSVTVHASLVALHQPLISFPRPVKTTWLNRNAPAQNKDSVIPTLESVVFGINYTKQLSPDGCSFVIADSFLRHAYRFHYTLCAALLLAFKGLHSYFITVTEELPSCQKLELAKANMQVLYERLLRRKQPATQKDTCLEEMDVEARLTELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVITLHEELRLLLAQEHHTLRVRRFSEAFFCFEHPREAAIAYQELHAQSHLQMCTAIKNTSFCSSLPPLPIECSELDGDLNSLPIIFEDRYLDSVTEDLDAPWMGIQSLQRSESSRMDKCETEESTLAGLSSPELKIRPAGTSSFWSTEGEKQLTKSLKGKNEESNKSKVKVTKLMKTMKPENTKKLIKQNSKDLVVLVGYKCLKSTASNDLFKSFEGNPTHSQKEGLDPTIYGYNFDPKTYIRQTSQKEASYLPANTERTEQKSPDIENMQPDLFDPLNSGSLNLCANLSISGKLGISQDDSEIAQVEHSVASRSSSDDCHDRQSAPTSGVRTIEVKPCNKDCFSGEKVTVKIGPWTELQQDEIFVDNLQLPNFESLDSNGKSKPMEITLEKEALQEAKCHSVGESLAKLRNNQPASTREYHLVVSGDTIKLPDISATCASSRFSDSGVESEPSSVAAHPNPDIVFETVPGQGLFISERLFPQLLMKPDCNVKFSLGSHCTESTSAFSEIQSSLTSINSLPSDDELSPDENPKKSAVSECHLSDSKTVLNLGTTDMPKCDDRKKSSIILQQQSVIFSGHSDNETIEIHSLNSSTKDPLQFVFSDEDTSSDVKSSCSSKPNLDTVCKDSQSPDKSSDSVGTTVPLSSELACSGTPCVVSGSVSTRADSSEDRTVKRKNSDALNPKQMCSEAPAVTDETYLGTGDAFSASPDMVKQGLVENYFGCQSSTDVSDTWAISYSHSVNPQKETSGKEMSSPQRDQGKDEEEEEQDQQMVQNGYYEEADYSSLEAAGDAHCADGADLGGERLAKSERLAGDYLSDGITMPTVCTSGCLSFPSAPRESPCGVKYSSKSKCDAVTKQPSSASHSCTSSLSWYENSPKPQIQAFLQAKDELKQLKLPGFMYSDVPLLASSVPYFCVEEEEEDHYENGVHLIVCVHGLDGNSADLRLVKTYIELGLPGERIDFLMSERNQNDTFADFDSMTDRLLDEIIQYIQIYSLTVSKISFIGHSLGNLIIRSVLTRPRFKYYLSRLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSNKAGLHYFRNVVLVGSLQDRYVPYHSARIEMCKTALKDKQSGQIYSEMIHNLLRPVLQSKDCNLVRYNVINALPNTADSLIGRAAHIAVLDSEIFLEKFFLVAALKYFQ

Target ORF information:

RefSeq Version	XM_025294942.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis family with sequence similarity 135 member A (FAM135A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025294942.1

ORF Insert Sequence:

ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT 
CCGCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT 
TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG 
GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT 
GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA 
GATTATTCGG CAGATGATCT GAACGCCTTG CAGCTAATAA GCAGCCGCAC GCTGAAGCTG 
CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGCTAACG TCATGTTTGA TTATTTCCAC 
CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA 
AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA 
GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA 
TCACCAGATG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC 
CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT 
ACGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGCCAAAGC CAACATGCAG 
GTCCTTTATG AGCGCCTTCT CAGAAGAAAA CAGCCAGCAA CCCAGAAAGA CACCTGTCTC 
GAGGAAATGG ATGTAGAAGC TCGACTTACT GAATTATGTG AAGAAGTTAA GAAAATAGAA 
AATCCTGATG AACTGGCAGA ACTTATAAAT ATGAATCTTG CACAACTTTG CTCACTTTTA 
ATGGCTTTAT GGGGACAATT TCTGGAAGTT ATAACACTAC ATGAGGAACT AAGACTATTA 
CTAGCACAAG AGCACCATAC TCTGAGGGTG CGCAGGTTTT CTGAGGCCTT CTTTTGTTTT 
GAACATCCAA GAGAAGCTGC CATTGCATAC CAGGAACTTC ATGCTCAGAG TCATCTCCAG 
ATGTGCACCG CTATCAAAAA CACTTCCTTC TGCAGCTCTC TCCCACCCCT GCCTATTGAA 
TGTAGTGAAT TAGATGGAGA TCTCAATTCG TTACCTATTA TCTTTGAAGA TAGGTATTTA 
GATTCAGTTA CTGAAGACTT AGATGCACCC TGGATGGGAA TTCAGAGTCT TCAGAGATCA 
GAGTCCAGTA GAATGGATAA ATGTGAGACT GAAGAAAGCA CTCTAGCTGG ACTTTCCAGC 
CCAGAGTTGA AAATCAGACC TGCTGGGACC TCCAGTTTTT GGTCTACAGA AGGTGAAAAG 
CAGCTAACAA AGTCTCTGAA AGGAAAGAAT GAAGAATCAA ATAAATCCAA GGTTAAGGTT 
ACTAAGCTCA TGAAAACAAT GAAACCTGAA AACACAAAAA AATTAATAAA ACAGAACTCT 
AAGGATTTGG TGGTTTTGGT AGGCTACAAA TGCTTGAAAA GTACAGCATC AAATGATCTC 
TTTAAAAGCT TTGAAGGCAA CCCTACACAT AGTCAGAAGG AAGGTCTGGA CCCCACAATA 
TATGGATATA ATTTTGATCC AAAGACCTAC ATCAGACAGA CAAGTCAAAA GGAAGCTAGC 
TATTTGCCAG CTAATACAGA AAGAACTGAA CAAAAGTCTC CAGACATTGA AAATATGCAA 
CCAGACCTGT TTGATCCTTT GAACTCTGGC AGCCTAAATC TTTGTGCAAA TTTGTCCATT 
TCAGGTAAAC TTGGTATCTC CCAGGATGAT AGTGAAATTG CACAAGTGGA ACACAGTGTG 
GCATCCAGAA GCTCATCAGA CGATTGCCAT GATCGTCAGT CTGCCCCCAC TTCAGGAGTT 
AGGACAATTG AAGTTAAGCC ATGTAATAAA GACTGTTTCA GTGGAGAGAA AGTAACTGTT 
AAAATAGGAC CTTGGACAGA GCTTCAACAA GATGAAATAT TTGTGGATAA TTTACAACTG 
CCCAACTTTG AGTCCTTAGA CTCTAATGGT AAATCTAAAC CTATGGAAAT AACACTTGAA 
AAGGAAGCTT TGCAGGAAGC TAAGTGTCAT TCTGTCGGGG AATCGTTAGC TAAGTTAAGA 
AATAATCAAC CTGCTTCTAC AAGAGAATAC CACCTTGTAG TGAGTGGAGA CACCATTAAG 
TTACCAGACA TTAGTGCCAC GTGTGCCTCA TCTCGGTTCT CAGACTCAGG TGTGGAAAGT 
GAGCCAAGCT CTGTTGCAGC ACATCCAAAC CCTGACATAG TCTTTGAAAC TGTACCAGGG 
CAAGGTCTTT TCATCAGTGA AAGGTTATTT CCTCAGCTTT TGATGAAGCC TGACTGTAAT 
GTAAAGTTTT CACTAGGCAG TCACTGTACT GAGAGTACAA GTGCTTTCAG TGAGATCCAG 
TCGTCCTTAA CCTCCATAAA TTCTCTGCCT TCTGATGACG AGCTGTCACC TGATGAAAAT 
CCTAAGAAAT CTGCTGTATC TGAATGCCAC CTAAGTGATA GCAAAACTGT GTTAAATCTA 
GGAACAACTG ATATGCCAAA ATGTGACGAT AGGAAAAAGT CAAGTATTAT TTTGCAGCAG 
CAGAGTGTCA TATTTTCAGG GCATTCGGAC AATGAAACTA TAGAAATACA TTCCTTAAAT 
TCAAGCACTA AAGACCCTTT ACAATTTGTT TTTTCAGATG AAGATACTTC TAGTGATGTG 
AAAAGTAGTT GCAGCTCCAA ACCTAACTTG GACACTGTGT GTAAAGACTC CCAGAGTCCT 
GATAAATCCA GTGACTCTGT AGGAACCACA GTTCCGTTAA GTTCAGAACT GGCTTGTTCA 
GGCACCCCTT GTGTCGTTTC AGGTTCCGTT TCCACCAGGG CAGATAGCAG TGAAGATAGA 
ACTGTGAAAA GAAAAAACAG TGATGCATTA AATCCCAAAC AAATGTGTTC AGAAGCCCCT 
GCAGTTACAG ATGAAACTTA CCTGGGTACA GGTGATGCTT TTTCAGCCAG TCCTGATATG 
GTAAAGCAAG GGCTTGTGGA AAATTATTTT GGCTGTCAAA GCAGTACGGA TGTTTCTGAC 
ACATGGGCTA TTAGCTACAG CCATTCAGTT AACCCTCAGA AAGAAACTTC TGGGAAAGAA 
ATGAGTAGTC CTCAGCGGGA TCAGGGTAAA GATGAGGAAG AGGAAGAGCA GGATCAACAA 
ATGGTTCAAA ACGGGTATTA TGAGGAGGCA GATTACTCCT CTCTGGAGGC CGCGGGAGAT 
GCTCACTGTG CAGACGGAGC TGACCTGGGA GGAGAAAGAC TTGCCAAGTC TGAAAGACTG 
GCCGGCGACT ATCTGAGCGA TGGCATAACC ATGCCTACTG TCTGTACTTC TGGCTGTCTG 
TCCTTCCCGT CTGCACCACG AGAGTCCCCT TGTGGCGTTA AGTACTCTTC TAAGAGTAAA 
TGTGACGCTG TTACCAAGCA GCCGAGCAGC GCCTCCCACA GCTGCACCTC ATCGCTGTCC 
TGGTACGAAA ACTCGCCAAA GCCTCAGATC CAAGCCTTCC TTCAGGCAAA AGATGAATTG 
AAGCAACTTA AACTCCCCGG GTTCATGTAC AGCGATGTTC CTCTGCTGGC ATCCTCAGTC 
CCTTATTTTT GCGTGGAGGA GGAGGAGGAG GACCATTATG AAAATGGAGT GCATCTCATT 
GTCTGTGTGC ACGGCCTAGA CGGTAACAGT GCAGATCTCC GATTAGTAAA AACTTACATT 
GAACTTGGGC TGCCTGGGGA AAGAATTGAT TTTCTTATGT CTGAAAGAAA TCAGAATGAT 
ACTTTTGCTG ATTTTGATAG CATGACTGAT CGCCTTTTGG ATGAGATAAT ACAATATATT 
CAGATATATA GTCTAACAGT CTCAAAAATA AGCTTTATTG GACATTCTTT GGGCAATTTA 
ATAATCCGTT CAGTGCTTAC AAGACCACGG TTTAAATATT ACCTCAGCAG GCTTCATACC 
TTTCTTTCAC TTTCTGGACC TCACCTTGGT ACACTCTACA ATAGCAGTGC TCTTGTTAAT 
ACAGGTCTGT GGTTTATGCA GAAATGGAAA AAATCAGGTT CTCTTTTACA ATTGACATGT 
CGTGATCATT CAGACCCTCG CCAAACTTTC TTATACAAGC TTAGTAATAA AGCAGGGCTT 
CACTACTTCA GAAATGTTGT CCTAGTGGGA TCTCTGCAAG ATCGCTATGT TCCTTATCAT 
TCTGCCCGCA TTGAAATGTG CAAGACAGCT TTGAAGGACA AACAGTCAGG ACAGATCTAT 
TCAGAAATGA TCCACAACTT ACTTCGCCCG GTTCTGCAAA GCAAGGACTG TAACTTGGTT 
CGATATAATG TCATCAATGC ATTGCCCAAT ACAGCTGATT CACTCATTGG CAGAGCTGCA 
CATATAGCTG TTCTGGATTC AGAAATATTT TTAGAGAAAT TCTTTCTGGT TGCTGCCCTC 
AAATATTTCC AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FAM135A ( XM_006051057.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006051119.1 Bubalus bubalis family with sequence similarity 135, member A (FAM135A), transcript variant X1, mRNA.

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CloneID	OBu09036
Clone ID Related Accession (Same CDS sequence)	XM_025294942.1 , XM_006051057.1 , XM_025294941.1
Accession Version	XM_006051057.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	protein FAM135A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT 
CCGCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT 
TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG 
GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT 
GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA 
GATTATTCGG CAGATGATCT GAACGCCTTG CAGCTAATAA GCAGCCGCAC GCTGAAGCTG 
CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGCTAACG TCATGTTTGA TTATTTCCAC 
CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA 
AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA 
GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA 
TCACCAGATG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC 
CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT 
ACGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGCCAAAGC CAACATGCAG 
GTCCTTTATG AGCGCCTTCT CAGAAGAAAA CAGCCAGCAA CCCAGAAAGA CACCTGTCTC 
GAGGAAATGG ATGTAGAAGC TCGACTTACT GAATTATGTG AAGAAGTTAA GAAAATAGAA 
AATCCTGATG AACTGGCAGA ACTTATAAAT ATGAATCTTG CACAACTTTG CTCACTTTTA 
ATGGCTTTAT GGGGACAATT TCTGGAAGTT ATAACACTAC ATGAGGAACT AAGACTATTA 
CTAGCACAAG AGCACCATAC TCTGAGGGTG CGCAGGTTTT CTGAGGCCTT CTTTTGTTTT 
GAACATCCAA GAGAAGCTGC CATTGCATAC CAGGAACTTC ATGCTCAGAG TCATCTCCAG 
ATGTGCACCG CTATCAAAAA CACTTCCTTC TGCAGCTCTC TCCCACCCCT GCCTATTGAA 
TGTAGTGAAT TAGATGGAGA TCTCAATTCG TTACCTATTA TCTTTGAAGA TAGGTATTTA 
GATTCAGTTA CTGAAGACTT AGATGCACCC TGGATGGGAA TTCAGAGTCT TCAGAGATCA 
GAGTCCAGTA GAATGGATAA ATGTGAGACT GAAGAAAGCA CTCTAGCTGG ACTTTCCAGC 
CCAGAGTTGA AAATCAGACC TGCTGGGACC TCCAGTTTTT GGTCTACAGA AGGTGAAAAG 
CAGCTAACAA AGTCTCTGAA AGGAAAGAAT GAAGAATCAA ATAAATCCAA GGTTAAGGTT 
ACTAAGCTCA TGAAAACAAT GAAACCTGAA AACACAAAAA AATTAATAAA ACAGAACTCT 
AAGGATTTGG TGGTTTTGGT AGGCTACAAA TGCTTGAAAA GTACAGCATC AAATGATCTC 
TTTAAAAGCT TTGAAGGCAA CCCTACACAT AGTCAGAAGG AAGGTCTGGA CCCCACAATA 
TATGGATATA ATTTTGATCC AAAGACCTAC ATCAGACAGA CAAGTCAAAA GGAAGCTAGC 
TATTTGCCAG CTAATACAGA AAGAACTGAA CAAAAGTCTC CAGACATTGA AAATATGCAA 
CCAGACCTGT TTGATCCTTT GAACTCTGGC AGCCTAAATC TTTGTGCAAA TTTGTCCATT 
TCAGGTAAAC TTGGTATCTC CCAGGATGAT AGTGAAATTG CACAAGTGGA ACACAGTGTG 
GCATCCAGAA GCTCATCAGA CGATTGCCAT GATCGTCAGT CTGCCCCCAC TTCAGGAGTT 
AGGACAATTG AAGTTAAGCC ATGTAATAAA GACTGTTTCA GTGGAGAGAA AGTAACTGTT 
AAAATAGGAC CTTGGACAGA GCTTCAACAA GATGAAATAT TTGTGGATAA TTTACAACTG 
CCCAACTTTG AGTCCTTAGA CTCTAATGGT AAATCTAAAC CTATGGAAAT AACACTTGAA 
AAGGAAGCTT TGCAGGAAGC TAAGTGTCAT TCTGTCGGGG AATCGTTAGC TAAGTTAAGA 
AATAATCAAC CTGCTTCTAC AAGAGAATAC CACCTTGTAG TGAGTGGAGA CACCATTAAG 
TTACCAGACA TTAGTGCCAC GTGTGCCTCA TCTCGGTTCT CAGACTCAGG TGTGGAAAGT 
GAGCCAAGCT CTGTTGCAGC ACATCCAAAC CCTGACATAG TCTTTGAAAC TGTACCAGGG 
CAAGGTCTTT TCATCAGTGA AAGGTTATTT CCTCAGCTTT TGATGAAGCC TGACTGTAAT 
GTAAAGTTTT CACTAGGCAG TCACTGTACT GAGAGTACAA GTGCTTTCAG TGAGATCCAG 
TCGTCCTTAA CCTCCATAAA TTCTCTGCCT TCTGATGACG AGCTGTCACC TGATGAAAAT 
CCTAAGAAAT CTGCTGTATC TGAATGCCAC CTAAGTGATA GCAAAACTGT GTTAAATCTA 
GGAACAACTG ATATGCCAAA ATGTGACGAT AGGAAAAAGT CAAGTATTAT TTTGCAGCAG 
CAGAGTGTCA TATTTTCAGG GCATTCGGAC AATGAAACTA TAGAAATACA TTCCTTAAAT 
TCAAGCACTA AAGACCCTTT ACAATTTGTT TTTTCAGATG AAGATACTTC TAGTGATGTG 
AAAAGTAGTT GCAGCTCCAA ACCTAACTTG GACACTGTGT GTAAAGACTC CCAGAGTCCT 
GATAAATCCA GTGACTCTGT AGGAACCACA GTTCCGTTAA GTTCAGAACT GGCTTGTTCA 
GGCACCCCTT GTGTCGTTTC AGGTTCCGTT TCCACCAGGG CAGATAGCAG TGAAGATAGA 
ACTGTGAAAA GAAAAAACAG TGATGCATTA AATCCCAAAC AAATGTGTTC AGAAGCCCCT 
GCAGTTACAG ATGAAACTTA CCTGGGTACA GGTGATGCTT TTTCAGCCAG TCCTGATATG 
GTAAAGCAAG GGCTTGTGGA AAATTATTTT GGCTGTCAAA GCAGTACGGA TGTTTCTGAC 
ACATGGGCTA TTAGCTACAG CCATTCAGTT AACCCTCAGA AAGAAACTTC TGGGAAAGAA 
ATGAGTAGTC CTCAGCGGGA TCAGGGTAAA GATGAGGAAG AGGAAGAGCA GGATCAACAA 
ATGGTTCAAA ACGGGTATTA TGAGGAGGCA GATTACTCCT CTCTGGAGGC CGCGGGAGAT 
GCTCACTGTG CAGACGGAGC TGACCTGGGA GGAGAAAGAC TTGCCAAGTC TGAAAGACTG 
GCCGGCGACT ATCTGAGCGA TGGCATAACC ATGCCTACTG TCTGTACTTC TGGCTGTCTG 
TCCTTCCCGT CTGCACCACG AGAGTCCCCT TGTGGCGTTA AGTACTCTTC TAAGAGTAAA 
TGTGACGCTG TTACCAAGCA GCCGAGCAGC GCCTCCCACA GCTGCACCTC ATCGCTGTCC 
TGGTACGAAA ACTCGCCAAA GCCTCAGATC CAAGCCTTCC TTCAGGCAAA AGATGAATTG 
AAGCAACTTA AACTCCCCGG GTTCATGTAC AGCGATGTTC CTCTGCTGGC ATCCTCAGTC 
CCTTATTTTT GCGTGGAGGA GGAGGAGGAG GACCATTATG AAAATGGAGT GCATCTCATT 
GTCTGTGTGC ACGGCCTAGA CGGTAACAGT GCAGATCTCC GATTAGTAAA AACTTACATT 
GAACTTGGGC TGCCTGGGGA AAGAATTGAT TTTCTTATGT CTGAAAGAAA TCAGAATGAT 
ACTTTTGCTG ATTTTGATAG CATGACTGAT CGCCTTTTGG ATGAGATAAT ACAATATATT 
CAGATATATA GTCTAACAGT CTCAAAAATA AGCTTTATTG GACATTCTTT GGGCAATTTA 
ATAATCCGTT CAGTGCTTAC AAGACCACGG TTTAAATATT ACCTCAGCAG GCTTCATACC 
TTTCTTTCAC TTTCTGGACC TCACCTTGGT ACACTCTACA ATAGCAGTGC TCTTGTTAAT 
ACAGGTCTGT GGTTTATGCA GAAATGGAAA AAATCAGGTT CTCTTTTACA ATTGACATGT 
CGTGATCATT CAGACCCTCG CCAAACTTTC TTATACAAGC TTAGTAATAA AGCAGGGCTT 
CACTACTTCA GAAATGTTGT CCTAGTGGGA TCTCTGCAAG ATCGCTATGT TCCTTATCAT 
TCTGCCCGCA TTGAAATGTG CAAGACAGCT TTGAAGGACA AACAGTCAGG ACAGATCTAT 
TCAGAAATGA TCCACAACTT ACTTCGCCCG GTTCTGCAAA GCAAGGACTG TAACTTGGTT 
CGATATAATG TCATCAATGC ATTGCCCAAT ACAGCTGATT CACTCATTGG CAGAGCTGCA 
CATATAGCTG TTCTGGATTC AGAAATATTT TTAGAGAAAT TCTTTCTGGT TGCTGCCCTC 
AAATATTTCC AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006051119.1
CDS	225..4859
Translation	MTEVQAMVEFSVELNKFYNVDLFQRGFYQIRASMKIPPRVPHRVEASLLHAAGMTLAFPASVHDSLICSKTFQILYKNEEVVLNDVMIFKVKMLLDEKKIEETLEEMNFLLSLDLHFTDGDYSADDLNALQLISSRTLKLHFSLQRGLHHHANVMFDYFHLSVVSVTVHASLVALHQPLISFPRPVKTTWLNRNAPAQNKDSVIPTLESVVFGINYTKQLSPDGCSFVIADSFLRHAYRFHYTLCAALLLAFKGLHSYFITVTEELPSCQKLELAKANMQVLYERLLRRKQPATQKDTCLEEMDVEARLTELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVITLHEELRLLLAQEHHTLRVRRFSEAFFCFEHPREAAIAYQELHAQSHLQMCTAIKNTSFCSSLPPLPIECSELDGDLNSLPIIFEDRYLDSVTEDLDAPWMGIQSLQRSESSRMDKCETEESTLAGLSSPELKIRPAGTSSFWSTEGEKQLTKSLKGKNEESNKSKVKVTKLMKTMKPENTKKLIKQNSKDLVVLVGYKCLKSTASNDLFKSFEGNPTHSQKEGLDPTIYGYNFDPKTYIRQTSQKEASYLPANTERTEQKSPDIENMQPDLFDPLNSGSLNLCANLSISGKLGISQDDSEIAQVEHSVASRSSSDDCHDRQSAPTSGVRTIEVKPCNKDCFSGEKVTVKIGPWTELQQDEIFVDNLQLPNFESLDSNGKSKPMEITLEKEALQEAKCHSVGESLAKLRNNQPASTREYHLVVSGDTIKLPDISATCASSRFSDSGVESEPSSVAAHPNPDIVFETVPGQGLFISERLFPQLLMKPDCNVKFSLGSHCTESTSAFSEIQSSLTSINSLPSDDELSPDENPKKSAVSECHLSDSKTVLNLGTTDMPKCDDRKKSSIILQQQSVIFSGHSDNETIEIHSLNSSTKDPLQFVFSDEDTSSDVKSSCSSKPNLDTVCKDSQSPDKSSDSVGTTVPLSSELACSGTPCVVSGSVSTRADSSEDRTVKRKNSDALNPKQMCSEAPAVTDETYLGTGDAFSASPDMVKQGLVENYFGCQSSTDVSDTWAISYSHSVNPQKETSGKEMSSPQRDQGKDEEEEEQDQQMVQNGYYEEADYSSLEAAGDAHCADGADLGGERLAKSERLAGDYLSDGITMPTVCTSGCLSFPSAPRESPCGVKYSSKSKCDAVTKQPSSASHSCTSSLSWYENSPKPQIQAFLQAKDELKQLKLPGFMYSDVPLLASSVPYFCVEEEEEDHYENGVHLIVCVHGLDGNSADLRLVKTYIELGLPGERIDFLMSERNQNDTFADFDSMTDRLLDEIIQYIQIYSLTVSKISFIGHSLGNLIIRSVLTRPRFKYYLSRLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSNKAGLHYFRNVVLVGSLQDRYVPYHSARIEMCKTALKDKQSGQIYSEMIHNLLRPVLQSKDCNLVRYNVINALPNTADSLIGRAAHIAVLDSEIFLEKFFLVAALKYFQ

Target ORF information:

RefSeq Version	XM_006051057.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis family with sequence similarity 135, member A (FAM135A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006051057.1

ORF Insert Sequence:

ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT 
CCGCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT 
TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG 
GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT 
GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA 
GATTATTCGG CAGATGATCT GAACGCCTTG CAGCTAATAA GCAGCCGCAC GCTGAAGCTG 
CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGCTAACG TCATGTTTGA TTATTTCCAC 
CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA 
AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA 
GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA 
TCACCAGATG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC 
CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT 
ACGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGCCAAAGC CAACATGCAG 
GTCCTTTATG AGCGCCTTCT CAGAAGAAAA CAGCCAGCAA CCCAGAAAGA CACCTGTCTC 
GAGGAAATGG ATGTAGAAGC TCGACTTACT GAATTATGTG AAGAAGTTAA GAAAATAGAA 
AATCCTGATG AACTGGCAGA ACTTATAAAT ATGAATCTTG CACAACTTTG CTCACTTTTA 
ATGGCTTTAT GGGGACAATT TCTGGAAGTT ATAACACTAC ATGAGGAACT AAGACTATTA 
CTAGCACAAG AGCACCATAC TCTGAGGGTG CGCAGGTTTT CTGAGGCCTT CTTTTGTTTT 
GAACATCCAA GAGAAGCTGC CATTGCATAC CAGGAACTTC ATGCTCAGAG TCATCTCCAG 
ATGTGCACCG CTATCAAAAA CACTTCCTTC TGCAGCTCTC TCCCACCCCT GCCTATTGAA 
TGTAGTGAAT TAGATGGAGA TCTCAATTCG TTACCTATTA TCTTTGAAGA TAGGTATTTA 
GATTCAGTTA CTGAAGACTT AGATGCACCC TGGATGGGAA TTCAGAGTCT TCAGAGATCA 
GAGTCCAGTA GAATGGATAA ATGTGAGACT GAAGAAAGCA CTCTAGCTGG ACTTTCCAGC 
CCAGAGTTGA AAATCAGACC TGCTGGGACC TCCAGTTTTT GGTCTACAGA AGGTGAAAAG 
CAGCTAACAA AGTCTCTGAA AGGAAAGAAT GAAGAATCAA ATAAATCCAA GGTTAAGGTT 
ACTAAGCTCA TGAAAACAAT GAAACCTGAA AACACAAAAA AATTAATAAA ACAGAACTCT 
AAGGATTTGG TGGTTTTGGT AGGCTACAAA TGCTTGAAAA GTACAGCATC AAATGATCTC 
TTTAAAAGCT TTGAAGGCAA CCCTACACAT AGTCAGAAGG AAGGTCTGGA CCCCACAATA 
TATGGATATA ATTTTGATCC AAAGACCTAC ATCAGACAGA CAAGTCAAAA GGAAGCTAGC 
TATTTGCCAG CTAATACAGA AAGAACTGAA CAAAAGTCTC CAGACATTGA AAATATGCAA 
CCAGACCTGT TTGATCCTTT GAACTCTGGC AGCCTAAATC TTTGTGCAAA TTTGTCCATT 
TCAGGTAAAC TTGGTATCTC CCAGGATGAT AGTGAAATTG CACAAGTGGA ACACAGTGTG 
GCATCCAGAA GCTCATCAGA CGATTGCCAT GATCGTCAGT CTGCCCCCAC TTCAGGAGTT 
AGGACAATTG AAGTTAAGCC ATGTAATAAA GACTGTTTCA GTGGAGAGAA AGTAACTGTT 
AAAATAGGAC CTTGGACAGA GCTTCAACAA GATGAAATAT TTGTGGATAA TTTACAACTG 
CCCAACTTTG AGTCCTTAGA CTCTAATGGT AAATCTAAAC CTATGGAAAT AACACTTGAA 
AAGGAAGCTT TGCAGGAAGC TAAGTGTCAT TCTGTCGGGG AATCGTTAGC TAAGTTAAGA 
AATAATCAAC CTGCTTCTAC AAGAGAATAC CACCTTGTAG TGAGTGGAGA CACCATTAAG 
TTACCAGACA TTAGTGCCAC GTGTGCCTCA TCTCGGTTCT CAGACTCAGG TGTGGAAAGT 
GAGCCAAGCT CTGTTGCAGC ACATCCAAAC CCTGACATAG TCTTTGAAAC TGTACCAGGG 
CAAGGTCTTT TCATCAGTGA AAGGTTATTT CCTCAGCTTT TGATGAAGCC TGACTGTAAT 
GTAAAGTTTT CACTAGGCAG TCACTGTACT GAGAGTACAA GTGCTTTCAG TGAGATCCAG 
TCGTCCTTAA CCTCCATAAA TTCTCTGCCT TCTGATGACG AGCTGTCACC TGATGAAAAT 
CCTAAGAAAT CTGCTGTATC TGAATGCCAC CTAAGTGATA GCAAAACTGT GTTAAATCTA 
GGAACAACTG ATATGCCAAA ATGTGACGAT AGGAAAAAGT CAAGTATTAT TTTGCAGCAG 
CAGAGTGTCA TATTTTCAGG GCATTCGGAC AATGAAACTA TAGAAATACA TTCCTTAAAT 
TCAAGCACTA AAGACCCTTT ACAATTTGTT TTTTCAGATG AAGATACTTC TAGTGATGTG 
AAAAGTAGTT GCAGCTCCAA ACCTAACTTG GACACTGTGT GTAAAGACTC CCAGAGTCCT 
GATAAATCCA GTGACTCTGT AGGAACCACA GTTCCGTTAA GTTCAGAACT GGCTTGTTCA 
GGCACCCCTT GTGTCGTTTC AGGTTCCGTT TCCACCAGGG CAGATAGCAG TGAAGATAGA 
ACTGTGAAAA GAAAAAACAG TGATGCATTA AATCCCAAAC AAATGTGTTC AGAAGCCCCT 
GCAGTTACAG ATGAAACTTA CCTGGGTACA GGTGATGCTT TTTCAGCCAG TCCTGATATG 
GTAAAGCAAG GGCTTGTGGA AAATTATTTT GGCTGTCAAA GCAGTACGGA TGTTTCTGAC 
ACATGGGCTA TTAGCTACAG CCATTCAGTT AACCCTCAGA AAGAAACTTC TGGGAAAGAA 
ATGAGTAGTC CTCAGCGGGA TCAGGGTAAA GATGAGGAAG AGGAAGAGCA GGATCAACAA 
ATGGTTCAAA ACGGGTATTA TGAGGAGGCA GATTACTCCT CTCTGGAGGC CGCGGGAGAT 
GCTCACTGTG CAGACGGAGC TGACCTGGGA GGAGAAAGAC TTGCCAAGTC TGAAAGACTG 
GCCGGCGACT ATCTGAGCGA TGGCATAACC ATGCCTACTG TCTGTACTTC TGGCTGTCTG 
TCCTTCCCGT CTGCACCACG AGAGTCCCCT TGTGGCGTTA AGTACTCTTC TAAGAGTAAA 
TGTGACGCTG TTACCAAGCA GCCGAGCAGC GCCTCCCACA GCTGCACCTC ATCGCTGTCC 
TGGTACGAAA ACTCGCCAAA GCCTCAGATC CAAGCCTTCC TTCAGGCAAA AGATGAATTG 
AAGCAACTTA AACTCCCCGG GTTCATGTAC AGCGATGTTC CTCTGCTGGC ATCCTCAGTC 
CCTTATTTTT GCGTGGAGGA GGAGGAGGAG GACCATTATG AAAATGGAGT GCATCTCATT 
GTCTGTGTGC ACGGCCTAGA CGGTAACAGT GCAGATCTCC GATTAGTAAA AACTTACATT 
GAACTTGGGC TGCCTGGGGA AAGAATTGAT TTTCTTATGT CTGAAAGAAA TCAGAATGAT 
ACTTTTGCTG ATTTTGATAG CATGACTGAT CGCCTTTTGG ATGAGATAAT ACAATATATT 
CAGATATATA GTCTAACAGT CTCAAAAATA AGCTTTATTG GACATTCTTT GGGCAATTTA 
ATAATCCGTT CAGTGCTTAC AAGACCACGG TTTAAATATT ACCTCAGCAG GCTTCATACC 
TTTCTTTCAC TTTCTGGACC TCACCTTGGT ACACTCTACA ATAGCAGTGC TCTTGTTAAT 
ACAGGTCTGT GGTTTATGCA GAAATGGAAA AAATCAGGTT CTCTTTTACA ATTGACATGT 
CGTGATCATT CAGACCCTCG CCAAACTTTC TTATACAAGC TTAGTAATAA AGCAGGGCTT 
CACTACTTCA GAAATGTTGT CCTAGTGGGA TCTCTGCAAG ATCGCTATGT TCCTTATCAT 
TCTGCCCGCA TTGAAATGTG CAAGACAGCT TTGAAGGACA AACAGTCAGG ACAGATCTAT 
TCAGAAATGA TCCACAACTT ACTTCGCCCG GTTCTGCAAA GCAAGGACTG TAACTTGGTT 
CGATATAATG TCATCAATGC ATTGCCCAAT ACAGCTGATT CACTCATTGG CAGAGCTGCA 
CATATAGCTG TTCTGGATTC AGAAATATTT TTAGAGAAAT TCTTTCTGGT TGCTGCCCTC 
AAATATTTCC AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FAM135A ( XM_025294941.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025150726.1 Bubalus bubalis family with sequence similarity 135 member A (FAM135A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu09036
Clone ID Related Accession (Same CDS sequence)	XM_025294942.1 , XM_006051057.1 , XM_025294941.1
Accession Version	XM_025294941.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	protein FAM135A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037554.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT 
CCGCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT 
TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG 
GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT 
GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA 
GATTATTCGG CAGATGATCT GAACGCCTTG CAGCTAATAA GCAGCCGCAC GCTGAAGCTG 
CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGCTAACG TCATGTTTGA TTATTTCCAC 
CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA 
AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA 
GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA 
TCACCAGATG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC 
CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT 
ACGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGCCAAAGC CAACATGCAG 
GTCCTTTATG AGCGCCTTCT CAGAAGAAAA CAGCCAGCAA CCCAGAAAGA CACCTGTCTC 
GAGGAAATGG ATGTAGAAGC TCGACTTACT GAATTATGTG AAGAAGTTAA GAAAATAGAA 
AATCCTGATG AACTGGCAGA ACTTATAAAT ATGAATCTTG CACAACTTTG CTCACTTTTA 
ATGGCTTTAT GGGGACAATT TCTGGAAGTT ATAACACTAC ATGAGGAACT AAGACTATTA 
CTAGCACAAG AGCACCATAC TCTGAGGGTG CGCAGGTTTT CTGAGGCCTT CTTTTGTTTT 
GAACATCCAA GAGAAGCTGC CATTGCATAC CAGGAACTTC ATGCTCAGAG TCATCTCCAG 
ATGTGCACCG CTATCAAAAA CACTTCCTTC TGCAGCTCTC TCCCACCCCT GCCTATTGAA 
TGTAGTGAAT TAGATGGAGA TCTCAATTCG TTACCTATTA TCTTTGAAGA TAGGTATTTA 
GATTCAGTTA CTGAAGACTT AGATGCACCC TGGATGGGAA TTCAGAGTCT TCAGAGATCA 
GAGTCCAGTA GAATGGATAA ATGTGAGACT GAAGAAAGCA CTCTAGCTGG ACTTTCCAGC 
CCAGAGTTGA AAATCAGACC TGCTGGGACC TCCAGTTTTT GGTCTACAGA AGGTGAAAAG 
CAGCTAACAA AGTCTCTGAA AGGAAAGAAT GAAGAATCAA ATAAATCCAA GGTTAAGGTT 
ACTAAGCTCA TGAAAACAAT GAAACCTGAA AACACAAAAA AATTAATAAA ACAGAACTCT 
AAGGATTTGG TGGTTTTGGT AGGCTACAAA TGCTTGAAAA GTACAGCATC AAATGATCTC 
TTTAAAAGCT TTGAAGGCAA CCCTACACAT AGTCAGAAGG AAGGTCTGGA CCCCACAATA 
TATGGATATA ATTTTGATCC AAAGACCTAC ATCAGACAGA CAAGTCAAAA GGAAGCTAGC 
TATTTGCCAG CTAATACAGA AAGAACTGAA CAAAAGTCTC CAGACATTGA AAATATGCAA 
CCAGACCTGT TTGATCCTTT GAACTCTGGC AGCCTAAATC TTTGTGCAAA TTTGTCCATT 
TCAGGTAAAC TTGGTATCTC CCAGGATGAT AGTGAAATTG CACAAGTGGA ACACAGTGTG 
GCATCCAGAA GCTCATCAGA CGATTGCCAT GATCGTCAGT CTGCCCCCAC TTCAGGAGTT 
AGGACAATTG AAGTTAAGCC ATGTAATAAA GACTGTTTCA GTGGAGAGAA AGTAACTGTT 
AAAATAGGAC CTTGGACAGA GCTTCAACAA GATGAAATAT TTGTGGATAA TTTACAACTG 
CCCAACTTTG AGTCCTTAGA CTCTAATGGT AAATCTAAAC CTATGGAAAT AACACTTGAA 
AAGGAAGCTT TGCAGGAAGC TAAGTGTCAT TCTGTCGGGG AATCGTTAGC TAAGTTAAGA 
AATAATCAAC CTGCTTCTAC AAGAGAATAC CACCTTGTAG TGAGTGGAGA CACCATTAAG 
TTACCAGACA TTAGTGCCAC GTGTGCCTCA TCTCGGTTCT CAGACTCAGG TGTGGAAAGT 
GAGCCAAGCT CTGTTGCAGC ACATCCAAAC CCTGACATAG TCTTTGAAAC TGTACCAGGG 
CAAGGTCTTT TCATCAGTGA AAGGTTATTT CCTCAGCTTT TGATGAAGCC TGACTGTAAT 
GTAAAGTTTT CACTAGGCAG TCACTGTACT GAGAGTACAA GTGCTTTCAG TGAGATCCAG 
TCGTCCTTAA CCTCCATAAA TTCTCTGCCT TCTGATGACG AGCTGTCACC TGATGAAAAT 
CCTAAGAAAT CTGCTGTATC TGAATGCCAC CTAAGTGATA GCAAAACTGT GTTAAATCTA 
GGAACAACTG ATATGCCAAA ATGTGACGAT AGGAAAAAGT CAAGTATTAT TTTGCAGCAG 
CAGAGTGTCA TATTTTCAGG GCATTCGGAC AATGAAACTA TAGAAATACA TTCCTTAAAT 
TCAAGCACTA AAGACCCTTT ACAATTTGTT TTTTCAGATG AAGATACTTC TAGTGATGTG 
AAAAGTAGTT GCAGCTCCAA ACCTAACTTG GACACTGTGT GTAAAGACTC CCAGAGTCCT 
GATAAATCCA GTGACTCTGT AGGAACCACA GTTCCGTTAA GTTCAGAACT GGCTTGTTCA 
GGCACCCCTT GTGTCGTTTC AGGTTCCGTT TCCACCAGGG CAGATAGCAG TGAAGATAGA 
ACTGTGAAAA GAAAAAACAG TGATGCATTA AATCCCAAAC AAATGTGTTC AGAAGCCCCT 
GCAGTTACAG ATGAAACTTA CCTGGGTACA GGTGATGCTT TTTCAGCCAG TCCTGATATG 
GTAAAGCAAG GGCTTGTGGA AAATTATTTT GGCTGTCAAA GCAGTACGGA TGTTTCTGAC 
ACATGGGCTA TTAGCTACAG CCATTCAGTT AACCCTCAGA AAGAAACTTC TGGGAAAGAA 
ATGAGTAGTC CTCAGCGGGA TCAGGGTAAA GATGAGGAAG AGGAAGAGCA GGATCAACAA 
ATGGTTCAAA ACGGGTATTA TGAGGAGGCA GATTACTCCT CTCTGGAGGC CGCGGGAGAT 
GCTCACTGTG CAGACGGAGC TGACCTGGGA GGAGAAAGAC TTGCCAAGTC TGAAAGACTG 
GCCGGCGACT ATCTGAGCGA TGGCATAACC ATGCCTACTG TCTGTACTTC TGGCTGTCTG 
TCCTTCCCGT CTGCACCACG AGAGTCCCCT TGTGGCGTTA AGTACTCTTC TAAGAGTAAA 
TGTGACGCTG TTACCAAGCA GCCGAGCAGC GCCTCCCACA GCTGCACCTC ATCGCTGTCC 
TGGTACGAAA ACTCGCCAAA GCCTCAGATC CAAGCCTTCC TTCAGGCAAA AGATGAATTG 
AAGCAACTTA AACTCCCCGG GTTCATGTAC AGCGATGTTC CTCTGCTGGC ATCCTCAGTC 
CCTTATTTTT GCGTGGAGGA GGAGGAGGAG GACCATTATG AAAATGGAGT GCATCTCATT 
GTCTGTGTGC ACGGCCTAGA CGGTAACAGT GCAGATCTCC GATTAGTAAA AACTTACATT 
GAACTTGGGC TGCCTGGGGA AAGAATTGAT TTTCTTATGT CTGAAAGAAA TCAGAATGAT 
ACTTTTGCTG ATTTTGATAG CATGACTGAT CGCCTTTTGG ATGAGATAAT ACAATATATT 
CAGATATATA GTCTAACAGT CTCAAAAATA AGCTTTATTG GACATTCTTT GGGCAATTTA 
ATAATCCGTT CAGTGCTTAC AAGACCACGG TTTAAATATT ACCTCAGCAG GCTTCATACC 
TTTCTTTCAC TTTCTGGACC TCACCTTGGT ACACTCTACA ATAGCAGTGC TCTTGTTAAT 
ACAGGTCTGT GGTTTATGCA GAAATGGAAA AAATCAGGTT CTCTTTTACA ATTGACATGT 
CGTGATCATT CAGACCCTCG CCAAACTTTC TTATACAAGC TTAGTAATAA AGCAGGGCTT 
CACTACTTCA GAAATGTTGT CCTAGTGGGA TCTCTGCAAG ATCGCTATGT TCCTTATCAT 
TCTGCCCGCA TTGAAATGTG CAAGACAGCT TTGAAGGACA AACAGTCAGG ACAGATCTAT 
TCAGAAATGA TCCACAACTT ACTTCGCCCG GTTCTGCAAA GCAAGGACTG TAACTTGGTT 
CGATATAATG TCATCAATGC ATTGCCCAAT ACAGCTGATT CACTCATTGG CAGAGCTGCA 
CATATAGCTG TTCTGGATTC AGAAATATTT TTAGAGAAAT TCTTTCTGGT TGCTGCCCTC 
AAATATTTCC AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025150726.1
CDS	377..5011
Translation	MTEVQAMVEFSVELNKFYNVDLFQRGFYQIRASMKIPPRVPHRVEASLLHAAGMTLAFPASVHDSLICSKTFQILYKNEEVVLNDVMIFKVKMLLDEKKIEETLEEMNFLLSLDLHFTDGDYSADDLNALQLISSRTLKLHFSLQRGLHHHANVMFDYFHLSVVSVTVHASLVALHQPLISFPRPVKTTWLNRNAPAQNKDSVIPTLESVVFGINYTKQLSPDGCSFVIADSFLRHAYRFHYTLCAALLLAFKGLHSYFITVTEELPSCQKLELAKANMQVLYERLLRRKQPATQKDTCLEEMDVEARLTELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVITLHEELRLLLAQEHHTLRVRRFSEAFFCFEHPREAAIAYQELHAQSHLQMCTAIKNTSFCSSLPPLPIECSELDGDLNSLPIIFEDRYLDSVTEDLDAPWMGIQSLQRSESSRMDKCETEESTLAGLSSPELKIRPAGTSSFWSTEGEKQLTKSLKGKNEESNKSKVKVTKLMKTMKPENTKKLIKQNSKDLVVLVGYKCLKSTASNDLFKSFEGNPTHSQKEGLDPTIYGYNFDPKTYIRQTSQKEASYLPANTERTEQKSPDIENMQPDLFDPLNSGSLNLCANLSISGKLGISQDDSEIAQVEHSVASRSSSDDCHDRQSAPTSGVRTIEVKPCNKDCFSGEKVTVKIGPWTELQQDEIFVDNLQLPNFESLDSNGKSKPMEITLEKEALQEAKCHSVGESLAKLRNNQPASTREYHLVVSGDTIKLPDISATCASSRFSDSGVESEPSSVAAHPNPDIVFETVPGQGLFISERLFPQLLMKPDCNVKFSLGSHCTESTSAFSEIQSSLTSINSLPSDDELSPDENPKKSAVSECHLSDSKTVLNLGTTDMPKCDDRKKSSIILQQQSVIFSGHSDNETIEIHSLNSSTKDPLQFVFSDEDTSSDVKSSCSSKPNLDTVCKDSQSPDKSSDSVGTTVPLSSELACSGTPCVVSGSVSTRADSSEDRTVKRKNSDALNPKQMCSEAPAVTDETYLGTGDAFSASPDMVKQGLVENYFGCQSSTDVSDTWAISYSHSVNPQKETSGKEMSSPQRDQGKDEEEEEQDQQMVQNGYYEEADYSSLEAAGDAHCADGADLGGERLAKSERLAGDYLSDGITMPTVCTSGCLSFPSAPRESPCGVKYSSKSKCDAVTKQPSSASHSCTSSLSWYENSPKPQIQAFLQAKDELKQLKLPGFMYSDVPLLASSVPYFCVEEEEEDHYENGVHLIVCVHGLDGNSADLRLVKTYIELGLPGERIDFLMSERNQNDTFADFDSMTDRLLDEIIQYIQIYSLTVSKISFIGHSLGNLIIRSVLTRPRFKYYLSRLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSNKAGLHYFRNVVLVGSLQDRYVPYHSARIEMCKTALKDKQSGQIYSEMIHNLLRPVLQSKDCNLVRYNVINALPNTADSLIGRAAHIAVLDSEIFLEKFFLVAALKYFQ

Target ORF information:

RefSeq Version	XM_025294941.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis family with sequence similarity 135 member A (FAM135A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025294941.1

ORF Insert Sequence:

ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT 
CCGCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT 
TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG 
GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT 
GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA 
GATTATTCGG CAGATGATCT GAACGCCTTG CAGCTAATAA GCAGCCGCAC GCTGAAGCTG 
CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGCTAACG TCATGTTTGA TTATTTCCAC 
CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA 
AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA 
GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA 
TCACCAGATG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC 
CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT 
ACGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGCCAAAGC CAACATGCAG 
GTCCTTTATG AGCGCCTTCT CAGAAGAAAA CAGCCAGCAA CCCAGAAAGA CACCTGTCTC 
GAGGAAATGG ATGTAGAAGC TCGACTTACT GAATTATGTG AAGAAGTTAA GAAAATAGAA 
AATCCTGATG AACTGGCAGA ACTTATAAAT ATGAATCTTG CACAACTTTG CTCACTTTTA 
ATGGCTTTAT GGGGACAATT TCTGGAAGTT ATAACACTAC ATGAGGAACT AAGACTATTA 
CTAGCACAAG AGCACCATAC TCTGAGGGTG CGCAGGTTTT CTGAGGCCTT CTTTTGTTTT 
GAACATCCAA GAGAAGCTGC CATTGCATAC CAGGAACTTC ATGCTCAGAG TCATCTCCAG 
ATGTGCACCG CTATCAAAAA CACTTCCTTC TGCAGCTCTC TCCCACCCCT GCCTATTGAA 
TGTAGTGAAT TAGATGGAGA TCTCAATTCG TTACCTATTA TCTTTGAAGA TAGGTATTTA 
GATTCAGTTA CTGAAGACTT AGATGCACCC TGGATGGGAA TTCAGAGTCT TCAGAGATCA 
GAGTCCAGTA GAATGGATAA ATGTGAGACT GAAGAAAGCA CTCTAGCTGG ACTTTCCAGC 
CCAGAGTTGA AAATCAGACC TGCTGGGACC TCCAGTTTTT GGTCTACAGA AGGTGAAAAG 
CAGCTAACAA AGTCTCTGAA AGGAAAGAAT GAAGAATCAA ATAAATCCAA GGTTAAGGTT 
ACTAAGCTCA TGAAAACAAT GAAACCTGAA AACACAAAAA AATTAATAAA ACAGAACTCT 
AAGGATTTGG TGGTTTTGGT AGGCTACAAA TGCTTGAAAA GTACAGCATC AAATGATCTC 
TTTAAAAGCT TTGAAGGCAA CCCTACACAT AGTCAGAAGG AAGGTCTGGA CCCCACAATA 
TATGGATATA ATTTTGATCC AAAGACCTAC ATCAGACAGA CAAGTCAAAA GGAAGCTAGC 
TATTTGCCAG CTAATACAGA AAGAACTGAA CAAAAGTCTC CAGACATTGA AAATATGCAA 
CCAGACCTGT TTGATCCTTT GAACTCTGGC AGCCTAAATC TTTGTGCAAA TTTGTCCATT 
TCAGGTAAAC TTGGTATCTC CCAGGATGAT AGTGAAATTG CACAAGTGGA ACACAGTGTG 
GCATCCAGAA GCTCATCAGA CGATTGCCAT GATCGTCAGT CTGCCCCCAC TTCAGGAGTT 
AGGACAATTG AAGTTAAGCC ATGTAATAAA GACTGTTTCA GTGGAGAGAA AGTAACTGTT 
AAAATAGGAC CTTGGACAGA GCTTCAACAA GATGAAATAT TTGTGGATAA TTTACAACTG 
CCCAACTTTG AGTCCTTAGA CTCTAATGGT AAATCTAAAC CTATGGAAAT AACACTTGAA 
AAGGAAGCTT TGCAGGAAGC TAAGTGTCAT TCTGTCGGGG AATCGTTAGC TAAGTTAAGA 
AATAATCAAC CTGCTTCTAC AAGAGAATAC CACCTTGTAG TGAGTGGAGA CACCATTAAG 
TTACCAGACA TTAGTGCCAC GTGTGCCTCA TCTCGGTTCT CAGACTCAGG TGTGGAAAGT 
GAGCCAAGCT CTGTTGCAGC ACATCCAAAC CCTGACATAG TCTTTGAAAC TGTACCAGGG 
CAAGGTCTTT TCATCAGTGA AAGGTTATTT CCTCAGCTTT TGATGAAGCC TGACTGTAAT 
GTAAAGTTTT CACTAGGCAG TCACTGTACT GAGAGTACAA GTGCTTTCAG TGAGATCCAG 
TCGTCCTTAA CCTCCATAAA TTCTCTGCCT TCTGATGACG AGCTGTCACC TGATGAAAAT 
CCTAAGAAAT CTGCTGTATC TGAATGCCAC CTAAGTGATA GCAAAACTGT GTTAAATCTA 
GGAACAACTG ATATGCCAAA ATGTGACGAT AGGAAAAAGT CAAGTATTAT TTTGCAGCAG 
CAGAGTGTCA TATTTTCAGG GCATTCGGAC AATGAAACTA TAGAAATACA TTCCTTAAAT 
TCAAGCACTA AAGACCCTTT ACAATTTGTT TTTTCAGATG AAGATACTTC TAGTGATGTG 
AAAAGTAGTT GCAGCTCCAA ACCTAACTTG GACACTGTGT GTAAAGACTC CCAGAGTCCT 
GATAAATCCA GTGACTCTGT AGGAACCACA GTTCCGTTAA GTTCAGAACT GGCTTGTTCA 
GGCACCCCTT GTGTCGTTTC AGGTTCCGTT TCCACCAGGG CAGATAGCAG TGAAGATAGA 
ACTGTGAAAA GAAAAAACAG TGATGCATTA AATCCCAAAC AAATGTGTTC AGAAGCCCCT 
GCAGTTACAG ATGAAACTTA CCTGGGTACA GGTGATGCTT TTTCAGCCAG TCCTGATATG 
GTAAAGCAAG GGCTTGTGGA AAATTATTTT GGCTGTCAAA GCAGTACGGA TGTTTCTGAC 
ACATGGGCTA TTAGCTACAG CCATTCAGTT AACCCTCAGA AAGAAACTTC TGGGAAAGAA 
ATGAGTAGTC CTCAGCGGGA TCAGGGTAAA GATGAGGAAG AGGAAGAGCA GGATCAACAA 
ATGGTTCAAA ACGGGTATTA TGAGGAGGCA GATTACTCCT CTCTGGAGGC CGCGGGAGAT 
GCTCACTGTG CAGACGGAGC TGACCTGGGA GGAGAAAGAC TTGCCAAGTC TGAAAGACTG 
GCCGGCGACT ATCTGAGCGA TGGCATAACC ATGCCTACTG TCTGTACTTC TGGCTGTCTG 
TCCTTCCCGT CTGCACCACG AGAGTCCCCT TGTGGCGTTA AGTACTCTTC TAAGAGTAAA 
TGTGACGCTG TTACCAAGCA GCCGAGCAGC GCCTCCCACA GCTGCACCTC ATCGCTGTCC 
TGGTACGAAA ACTCGCCAAA GCCTCAGATC CAAGCCTTCC TTCAGGCAAA AGATGAATTG 
AAGCAACTTA AACTCCCCGG GTTCATGTAC AGCGATGTTC CTCTGCTGGC ATCCTCAGTC 
CCTTATTTTT GCGTGGAGGA GGAGGAGGAG GACCATTATG AAAATGGAGT GCATCTCATT 
GTCTGTGTGC ACGGCCTAGA CGGTAACAGT GCAGATCTCC GATTAGTAAA AACTTACATT 
GAACTTGGGC TGCCTGGGGA AAGAATTGAT TTTCTTATGT CTGAAAGAAA TCAGAATGAT 
ACTTTTGCTG ATTTTGATAG CATGACTGAT CGCCTTTTGG ATGAGATAAT ACAATATATT 
CAGATATATA GTCTAACAGT CTCAAAAATA AGCTTTATTG GACATTCTTT GGGCAATTTA 
ATAATCCGTT CAGTGCTTAC AAGACCACGG TTTAAATATT ACCTCAGCAG GCTTCATACC 
TTTCTTTCAC TTTCTGGACC TCACCTTGGT ACACTCTACA ATAGCAGTGC TCTTGTTAAT 
ACAGGTCTGT GGTTTATGCA GAAATGGAAA AAATCAGGTT CTCTTTTACA ATTGACATGT 
CGTGATCATT CAGACCCTCG CCAAACTTTC TTATACAAGC TTAGTAATAA AGCAGGGCTT 
CACTACTTCA GAAATGTTGT CCTAGTGGGA TCTCTGCAAG ATCGCTATGT TCCTTATCAT 
TCTGCCCGCA TTGAAATGTG CAAGACAGCT TTGAAGGACA AACAGTCAGG ACAGATCTAT 
TCAGAAATGA TCCACAACTT ACTTCGCCCG GTTCTGCAAA GCAAGGACTG TAACTTGGTT 
CGATATAATG TCATCAATGC ATTGCCCAAT ACAGCTGATT CACTCATTGG CAGAGCTGCA 
CATATAGCTG TTCTGGATTC AGAAATATTT TTAGAGAAAT TCTTTCTGGT TGCTGCCCTC 
AAATATTTCC AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FAM135A ( XM_006051058.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006051120.1 Bubalus bubalis family with sequence similarity 135, member A (FAM135A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu09037
Clone ID Related Accession (Same CDS sequence)	XM_006051058.1 , XM_025294943.1
Accession Version	XM_006051058.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	protein FAM135A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT 
CCGCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT 
TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG 
GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT 
GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA 
GATTATTCGG CAGATGATCT GAACGCCTTG CAGCTAATAA GCAGCCGCAC GCTGAAGCTG 
CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGCTAACG TCATGTTTGA TTATTTCCAC 
CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA 
AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA 
GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA 
TCACCAGATG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC 
CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT 
ACGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGAGGAAAT GGATGTAGAA 
GCTCGACTTA CTGAATTATG TGAAGAAGTT AAGAAAATAG AAAATCCTGA TGAACTGGCA 
GAACTTATAA ATATGAATCT TGCACAACTT TGCTCACTTT TAATGGCTTT ATGGGGACAA 
TTTCTGGAAG TTATAACACT ACATGAGGAA CTAAGACTAT TACTAGCACA AGAGCACCAT 
ACTCTGAGGG TGCGCAGGTT TTCTGAGGCC TTCTTTTGTT TTGAACATCC AAGAGAAGCT 
GCCATTGCAT ACCAGGAACT TCATGCTCAG AGTCATCTCC AGATGTGCAC CGCTATCAAA 
AACACTTCCT TCTGCAGCTC TCTCCCACCC CTGCCTATTG AATGTAGTGA ATTAGATGGA 
GATCTCAATT CGTTACCTAT TATCTTTGAA GATAGGTATT TAGATTCAGT TACTGAAGAC 
TTAGATGCAC CCTGGATGGG AATTCAGAGT CTTCAGAGAT CAGAGTCCAG TAGAATGGAT 
AAATGTGAGA CTGAAGAAAG CACTCTAGCT GGACTTTCCA GCCCAGAGTT GAAAATCAGA 
CCTGCTGGGA CCTCCAGTTT TTGGTCTACA GAAGGTGAAA AGCAGCTAAC AAAGTCTCTG 
AAAGGAAAGA ATGAAGAATC AAATAAATCC AAGGTTAAGG TTACTAAGCT CATGAAAACA 
ATGAAACCTG AAAACACAAA AAAATTAATA AAACAGAACT CTAAGGATTT GGTGGTTTTG 
GTAGGCTACA AATGCTTGAA AAGTACAGCA TCAAATGATC TCTTTAAAAG CTTTGAAGGC 
AACCCTACAC ATAGTCAGAA GGAAGGTCTG GACCCCACAA TATATGGATA TAATTTTGAT 
CCAAAGACCT ACATCAGACA GACAAGTCAA AAGGAAGCTA GCTATTTGCC AGCTAATACA 
GAAAGAACTG AACAAAAGTC TCCAGACATT GAAAATATGC AACCAGACCT GTTTGATCCT 
TTGAACTCTG GCAGCCTAAA TCTTTGTGCA AATTTGTCCA TTTCAGGTAA ACTTGGTATC 
TCCCAGGATG ATAGTGAAAT TGCACAAGTG GAACACAGTG TGGCATCCAG AAGCTCATCA 
GACGATTGCC ATGATCGTCA GTCTGCCCCC ACTTCAGGAG TTAGGACAAT TGAAGTTAAG 
CCATGTAATA AAGACTGTTT CAGTGGAGAG AAAGTAACTG TTAAAATAGG ACCTTGGACA 
GAGCTTCAAC AAGATGAAAT ATTTGTGGAT AATTTACAAC TGCCCAACTT TGAGTCCTTA 
GACTCTAATG GTAAATCTAA ACCTATGGAA ATAACACTTG AAAAGGAAGC TTTGCAGGAA 
GCTAAGTGTC ATTCTGTCGG GGAATCGTTA GCTAAGTTAA GAAATAATCA ACCTGCTTCT 
ACAAGAGAAT ACCACCTTGT AGTGAGTGGA GACACCATTA AGTTACCAGA CATTAGTGCC 
ACGTGTGCCT CATCTCGGTT CTCAGACTCA GGTGTGGAAA GTGAGCCAAG CTCTGTTGCA 
GCACATCCAA ACCCTGACAT AGTCTTTGAA ACTGTACCAG GGCAAGGTCT TTTCATCAGT 
GAAAGGTTAT TTCCTCAGCT TTTGATGAAG CCTGACTGTA ATGTAAAGTT TTCACTAGGC 
AGTCACTGTA CTGAGAGTAC AAGTGCTTTC AGTGAGATCC AGTCGTCCTT AACCTCCATA 
AATTCTCTGC CTTCTGATGA CGAGCTGTCA CCTGATGAAA ATCCTAAGAA ATCTGCTGTA 
TCTGAATGCC ACCTAAGTGA TAGCAAAACT GTGTTAAATC TAGGAACAAC TGATATGCCA 
AAATGTGACG ATAGGAAAAA GTCAAGTATT ATTTTGCAGC AGCAGAGTGT CATATTTTCA 
GGGCATTCGG ACAATGAAAC TATAGAAATA CATTCCTTAA ATTCAAGCAC TAAAGACCCT 
TTACAATTTG TTTTTTCAGA TGAAGATACT TCTAGTGATG TGAAAAGTAG TTGCAGCTCC 
AAACCTAACT TGGACACTGT GTGTAAAGAC TCCCAGAGTC CTGATAAATC CAGTGACTCT 
GTAGGAACCA CAGTTCCGTT AAGTTCAGAA CTGGCTTGTT CAGGCACCCC TTGTGTCGTT 
TCAGGTTCCG TTTCCACCAG GGCAGATAGC AGTGAAGATA GAACTGTGAA AAGAAAAAAC 
AGTGATGCAT TAAATCCCAA ACAAATGTGT TCAGAAGCCC CTGCAGTTAC AGATGAAACT 
TACCTGGGTA CAGGTGATGC TTTTTCAGCC AGTCCTGATA TGGTAAAGCA AGGGCTTGTG 
GAAAATTATT TTGGCTGTCA AAGCAGTACG GATGTTTCTG ACACATGGGC TATTAGCTAC 
AGCCATTCAG TTAACCCTCA GAAAGAAACT TCTGGGAAAG AAATGAGTAG TCCTCAGCGG 
GATCAGGGTA AAGATGAGGA AGAGGAAGAG CAGGATCAAC AAATGGTTCA AAACGGGTAT 
TATGAGGAGG CAGATTACTC CTCTCTGGAG GCCGCGGGAG ATGCTCACTG TGCAGACGGA 
GCTGACCTGG GAGGAGAAAG ACTTGCCAAG TCTGAAAGAC TGGCCGGCGA CTATCTGAGC 
GATGGCATAA CCATGCCTAC TGTCTGTACT TCTGGCTGTC TGTCCTTCCC GTCTGCACCA 
CGAGAGTCCC CTTGTGGCGT TAAGTACTCT TCTAAGAGTA AATGTGACGC TGTTACCAAG 
CAGCCGAGCA GCGCCTCCCA CAGCTGCACC TCATCGCTGT CCTGGTACGA AAACTCGCCA 
AAGCCTCAGA TCCAAGCCTT CCTTCAGGCA AAAGATGAAT TGAAGCAACT TAAACTCCCC 
GGGTTCATGT ACAGCGATGT TCCTCTGCTG GCATCCTCAG TCCCTTATTT TTGCGTGGAG 
GAGGAGGAGG AGGACCATTA TGAAAATGGA GTGCATCTCA TTGTCTGTGT GCACGGCCTA 
GACGGTAACA GTGCAGATCT CCGATTAGTA AAAACTTACA TTGAACTTGG GCTGCCTGGG 
GAAAGAATTG ATTTTCTTAT GTCTGAAAGA AATCAGAATG ATACTTTTGC TGATTTTGAT 
AGCATGACTG ATCGCCTTTT GGATGAGATA ATACAATATA TTCAGATATA TAGTCTAACA 
GTCTCAAAAA TAAGCTTTAT TGGACATTCT TTGGGCAATT TAATAATCCG TTCAGTGCTT 
ACAAGACCAC GGTTTAAATA TTACCTCAGC AGGCTTCATA CCTTTCTTTC ACTTTCTGGA 
CCTCACCTTG GTACACTCTA CAATAGCAGT GCTCTTGTTA ATACAGGTCT GTGGTTTATG 
CAGAAATGGA AAAAATCAGG TTCTCTTTTA CAATTGACAT GTCGTGATCA TTCAGACCCT 
CGCCAAACTT TCTTATACAA GCTTAGTAAT AAAGCAGGGC TTCACTACTT CAGAAATGTT 
GTCCTAGTGG GATCTCTGCA AGATCGCTAT GTTCCTTATC ATTCTGCCCG CATTGAAATG 
TGCAAGACAG CTTTGAAGGA CAAACAGTCA GGACAGATCT ATTCAGAAAT GATCCACAAC 
TTACTTCGCC CGGTTCTGCA AAGCAAGGAC TGTAACTTGG TTCGATATAA TGTCATCAAT 
GCATTGCCCA ATACAGCTGA TTCACTCATT GGCAGAGCTG CACATATAGC TGTTCTGGAT 
TCAGAAATAT TTTTAGAGAA ATTCTTTCTG GTTGCTGCCC TCAAATATTT CCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006051120.1
CDS	225..4781
Translation	MTEVQAMVEFSVELNKFYNVDLFQRGFYQIRASMKIPPRVPHRVEASLLHAAGMTLAFPASVHDSLICSKTFQILYKNEEVVLNDVMIFKVKMLLDEKKIEETLEEMNFLLSLDLHFTDGDYSADDLNALQLISSRTLKLHFSLQRGLHHHANVMFDYFHLSVVSVTVHASLVALHQPLISFPRPVKTTWLNRNAPAQNKDSVIPTLESVVFGINYTKQLSPDGCSFVIADSFLRHAYRFHYTLCAALLLAFKGLHSYFITVTEELPSCQKLELEEMDVEARLTELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVITLHEELRLLLAQEHHTLRVRRFSEAFFCFEHPREAAIAYQELHAQSHLQMCTAIKNTSFCSSLPPLPIECSELDGDLNSLPIIFEDRYLDSVTEDLDAPWMGIQSLQRSESSRMDKCETEESTLAGLSSPELKIRPAGTSSFWSTEGEKQLTKSLKGKNEESNKSKVKVTKLMKTMKPENTKKLIKQNSKDLVVLVGYKCLKSTASNDLFKSFEGNPTHSQKEGLDPTIYGYNFDPKTYIRQTSQKEASYLPANTERTEQKSPDIENMQPDLFDPLNSGSLNLCANLSISGKLGISQDDSEIAQVEHSVASRSSSDDCHDRQSAPTSGVRTIEVKPCNKDCFSGEKVTVKIGPWTELQQDEIFVDNLQLPNFESLDSNGKSKPMEITLEKEALQEAKCHSVGESLAKLRNNQPASTREYHLVVSGDTIKLPDISATCASSRFSDSGVESEPSSVAAHPNPDIVFETVPGQGLFISERLFPQLLMKPDCNVKFSLGSHCTESTSAFSEIQSSLTSINSLPSDDELSPDENPKKSAVSECHLSDSKTVLNLGTTDMPKCDDRKKSSIILQQQSVIFSGHSDNETIEIHSLNSSTKDPLQFVFSDEDTSSDVKSSCSSKPNLDTVCKDSQSPDKSSDSVGTTVPLSSELACSGTPCVVSGSVSTRADSSEDRTVKRKNSDALNPKQMCSEAPAVTDETYLGTGDAFSASPDMVKQGLVENYFGCQSSTDVSDTWAISYSHSVNPQKETSGKEMSSPQRDQGKDEEEEEQDQQMVQNGYYEEADYSSLEAAGDAHCADGADLGGERLAKSERLAGDYLSDGITMPTVCTSGCLSFPSAPRESPCGVKYSSKSKCDAVTKQPSSASHSCTSSLSWYENSPKPQIQAFLQAKDELKQLKLPGFMYSDVPLLASSVPYFCVEEEEEDHYENGVHLIVCVHGLDGNSADLRLVKTYIELGLPGERIDFLMSERNQNDTFADFDSMTDRLLDEIIQYIQIYSLTVSKISFIGHSLGNLIIRSVLTRPRFKYYLSRLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSNKAGLHYFRNVVLVGSLQDRYVPYHSARIEMCKTALKDKQSGQIYSEMIHNLLRPVLQSKDCNLVRYNVINALPNTADSLIGRAAHIAVLDSEIFLEKFFLVAALKYFQ

Target ORF information:

RefSeq Version	XM_006051058.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis family with sequence similarity 135, member A (FAM135A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006051058.1

ORF Insert Sequence:

ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT 
CCGCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT 
TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG 
GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT 
GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA 
GATTATTCGG CAGATGATCT GAACGCCTTG CAGCTAATAA GCAGCCGCAC GCTGAAGCTG 
CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGCTAACG TCATGTTTGA TTATTTCCAC 
CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA 
AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA 
GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA 
TCACCAGATG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC 
CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT 
ACGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGAGGAAAT GGATGTAGAA 
GCTCGACTTA CTGAATTATG TGAAGAAGTT AAGAAAATAG AAAATCCTGA TGAACTGGCA 
GAACTTATAA ATATGAATCT TGCACAACTT TGCTCACTTT TAATGGCTTT ATGGGGACAA 
TTTCTGGAAG TTATAACACT ACATGAGGAA CTAAGACTAT TACTAGCACA AGAGCACCAT 
ACTCTGAGGG TGCGCAGGTT TTCTGAGGCC TTCTTTTGTT TTGAACATCC AAGAGAAGCT 
GCCATTGCAT ACCAGGAACT TCATGCTCAG AGTCATCTCC AGATGTGCAC CGCTATCAAA 
AACACTTCCT TCTGCAGCTC TCTCCCACCC CTGCCTATTG AATGTAGTGA ATTAGATGGA 
GATCTCAATT CGTTACCTAT TATCTTTGAA GATAGGTATT TAGATTCAGT TACTGAAGAC 
TTAGATGCAC CCTGGATGGG AATTCAGAGT CTTCAGAGAT CAGAGTCCAG TAGAATGGAT 
AAATGTGAGA CTGAAGAAAG CACTCTAGCT GGACTTTCCA GCCCAGAGTT GAAAATCAGA 
CCTGCTGGGA CCTCCAGTTT TTGGTCTACA GAAGGTGAAA AGCAGCTAAC AAAGTCTCTG 
AAAGGAAAGA ATGAAGAATC AAATAAATCC AAGGTTAAGG TTACTAAGCT CATGAAAACA 
ATGAAACCTG AAAACACAAA AAAATTAATA AAACAGAACT CTAAGGATTT GGTGGTTTTG 
GTAGGCTACA AATGCTTGAA AAGTACAGCA TCAAATGATC TCTTTAAAAG CTTTGAAGGC 
AACCCTACAC ATAGTCAGAA GGAAGGTCTG GACCCCACAA TATATGGATA TAATTTTGAT 
CCAAAGACCT ACATCAGACA GACAAGTCAA AAGGAAGCTA GCTATTTGCC AGCTAATACA 
GAAAGAACTG AACAAAAGTC TCCAGACATT GAAAATATGC AACCAGACCT GTTTGATCCT 
TTGAACTCTG GCAGCCTAAA TCTTTGTGCA AATTTGTCCA TTTCAGGTAA ACTTGGTATC 
TCCCAGGATG ATAGTGAAAT TGCACAAGTG GAACACAGTG TGGCATCCAG AAGCTCATCA 
GACGATTGCC ATGATCGTCA GTCTGCCCCC ACTTCAGGAG TTAGGACAAT TGAAGTTAAG 
CCATGTAATA AAGACTGTTT CAGTGGAGAG AAAGTAACTG TTAAAATAGG ACCTTGGACA 
GAGCTTCAAC AAGATGAAAT ATTTGTGGAT AATTTACAAC TGCCCAACTT TGAGTCCTTA 
GACTCTAATG GTAAATCTAA ACCTATGGAA ATAACACTTG AAAAGGAAGC TTTGCAGGAA 
GCTAAGTGTC ATTCTGTCGG GGAATCGTTA GCTAAGTTAA GAAATAATCA ACCTGCTTCT 
ACAAGAGAAT ACCACCTTGT AGTGAGTGGA GACACCATTA AGTTACCAGA CATTAGTGCC 
ACGTGTGCCT CATCTCGGTT CTCAGACTCA GGTGTGGAAA GTGAGCCAAG CTCTGTTGCA 
GCACATCCAA ACCCTGACAT AGTCTTTGAA ACTGTACCAG GGCAAGGTCT TTTCATCAGT 
GAAAGGTTAT TTCCTCAGCT TTTGATGAAG CCTGACTGTA ATGTAAAGTT TTCACTAGGC 
AGTCACTGTA CTGAGAGTAC AAGTGCTTTC AGTGAGATCC AGTCGTCCTT AACCTCCATA 
AATTCTCTGC CTTCTGATGA CGAGCTGTCA CCTGATGAAA ATCCTAAGAA ATCTGCTGTA 
TCTGAATGCC ACCTAAGTGA TAGCAAAACT GTGTTAAATC TAGGAACAAC TGATATGCCA 
AAATGTGACG ATAGGAAAAA GTCAAGTATT ATTTTGCAGC AGCAGAGTGT CATATTTTCA 
GGGCATTCGG ACAATGAAAC TATAGAAATA CATTCCTTAA ATTCAAGCAC TAAAGACCCT 
TTACAATTTG TTTTTTCAGA TGAAGATACT TCTAGTGATG TGAAAAGTAG TTGCAGCTCC 
AAACCTAACT TGGACACTGT GTGTAAAGAC TCCCAGAGTC CTGATAAATC CAGTGACTCT 
GTAGGAACCA CAGTTCCGTT AAGTTCAGAA CTGGCTTGTT CAGGCACCCC TTGTGTCGTT 
TCAGGTTCCG TTTCCACCAG GGCAGATAGC AGTGAAGATA GAACTGTGAA AAGAAAAAAC 
AGTGATGCAT TAAATCCCAA ACAAATGTGT TCAGAAGCCC CTGCAGTTAC AGATGAAACT 
TACCTGGGTA CAGGTGATGC TTTTTCAGCC AGTCCTGATA TGGTAAAGCA AGGGCTTGTG 
GAAAATTATT TTGGCTGTCA AAGCAGTACG GATGTTTCTG ACACATGGGC TATTAGCTAC 
AGCCATTCAG TTAACCCTCA GAAAGAAACT TCTGGGAAAG AAATGAGTAG TCCTCAGCGG 
GATCAGGGTA AAGATGAGGA AGAGGAAGAG CAGGATCAAC AAATGGTTCA AAACGGGTAT 
TATGAGGAGG CAGATTACTC CTCTCTGGAG GCCGCGGGAG ATGCTCACTG TGCAGACGGA 
GCTGACCTGG GAGGAGAAAG ACTTGCCAAG TCTGAAAGAC TGGCCGGCGA CTATCTGAGC 
GATGGCATAA CCATGCCTAC TGTCTGTACT TCTGGCTGTC TGTCCTTCCC GTCTGCACCA 
CGAGAGTCCC CTTGTGGCGT TAAGTACTCT TCTAAGAGTA AATGTGACGC TGTTACCAAG 
CAGCCGAGCA GCGCCTCCCA CAGCTGCACC TCATCGCTGT CCTGGTACGA AAACTCGCCA 
AAGCCTCAGA TCCAAGCCTT CCTTCAGGCA AAAGATGAAT TGAAGCAACT TAAACTCCCC 
GGGTTCATGT ACAGCGATGT TCCTCTGCTG GCATCCTCAG TCCCTTATTT TTGCGTGGAG 
GAGGAGGAGG AGGACCATTA TGAAAATGGA GTGCATCTCA TTGTCTGTGT GCACGGCCTA 
GACGGTAACA GTGCAGATCT CCGATTAGTA AAAACTTACA TTGAACTTGG GCTGCCTGGG 
GAAAGAATTG ATTTTCTTAT GTCTGAAAGA AATCAGAATG ATACTTTTGC TGATTTTGAT 
AGCATGACTG ATCGCCTTTT GGATGAGATA ATACAATATA TTCAGATATA TAGTCTAACA 
GTCTCAAAAA TAAGCTTTAT TGGACATTCT TTGGGCAATT TAATAATCCG TTCAGTGCTT 
ACAAGACCAC GGTTTAAATA TTACCTCAGC AGGCTTCATA CCTTTCTTTC ACTTTCTGGA 
CCTCACCTTG GTACACTCTA CAATAGCAGT GCTCTTGTTA ATACAGGTCT GTGGTTTATG 
CAGAAATGGA AAAAATCAGG TTCTCTTTTA CAATTGACAT GTCGTGATCA TTCAGACCCT 
CGCCAAACTT TCTTATACAA GCTTAGTAAT AAAGCAGGGC TTCACTACTT CAGAAATGTT 
GTCCTAGTGG GATCTCTGCA AGATCGCTAT GTTCCTTATC ATTCTGCCCG CATTGAAATG 
TGCAAGACAG CTTTGAAGGA CAAACAGTCA GGACAGATCT ATTCAGAAAT GATCCACAAC 
TTACTTCGCC CGGTTCTGCA AAGCAAGGAC TGTAACTTGG TTCGATATAA TGTCATCAAT 
GCATTGCCCA ATACAGCTGA TTCACTCATT GGCAGAGCTG CACATATAGC TGTTCTGGAT 
TCAGAAATAT TTTTAGAGAA ATTCTTTCTG GTTGCTGCCC TCAAATATTT CCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FAM135A ( XM_025294943.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025150728.1 Bubalus bubalis family with sequence similarity 135 member A (FAM135A), transcript variant X3, mRNA.

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CloneID	OBu09037
Clone ID Related Accession (Same CDS sequence)	XM_006051058.1 , XM_025294943.1
Accession Version	XM_025294943.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	protein FAM135A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037554.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT 
CCGCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT 
TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG 
GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT 
GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA 
GATTATTCGG CAGATGATCT GAACGCCTTG CAGCTAATAA GCAGCCGCAC GCTGAAGCTG 
CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGCTAACG TCATGTTTGA TTATTTCCAC 
CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA 
AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA 
GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA 
TCACCAGATG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC 
CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT 
ACGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGAGGAAAT GGATGTAGAA 
GCTCGACTTA CTGAATTATG TGAAGAAGTT AAGAAAATAG AAAATCCTGA TGAACTGGCA 
GAACTTATAA ATATGAATCT TGCACAACTT TGCTCACTTT TAATGGCTTT ATGGGGACAA 
TTTCTGGAAG TTATAACACT ACATGAGGAA CTAAGACTAT TACTAGCACA AGAGCACCAT 
ACTCTGAGGG TGCGCAGGTT TTCTGAGGCC TTCTTTTGTT TTGAACATCC AAGAGAAGCT 
GCCATTGCAT ACCAGGAACT TCATGCTCAG AGTCATCTCC AGATGTGCAC CGCTATCAAA 
AACACTTCCT TCTGCAGCTC TCTCCCACCC CTGCCTATTG AATGTAGTGA ATTAGATGGA 
GATCTCAATT CGTTACCTAT TATCTTTGAA GATAGGTATT TAGATTCAGT TACTGAAGAC 
TTAGATGCAC CCTGGATGGG AATTCAGAGT CTTCAGAGAT CAGAGTCCAG TAGAATGGAT 
AAATGTGAGA CTGAAGAAAG CACTCTAGCT GGACTTTCCA GCCCAGAGTT GAAAATCAGA 
CCTGCTGGGA CCTCCAGTTT TTGGTCTACA GAAGGTGAAA AGCAGCTAAC AAAGTCTCTG 
AAAGGAAAGA ATGAAGAATC AAATAAATCC AAGGTTAAGG TTACTAAGCT CATGAAAACA 
ATGAAACCTG AAAACACAAA AAAATTAATA AAACAGAACT CTAAGGATTT GGTGGTTTTG 
GTAGGCTACA AATGCTTGAA AAGTACAGCA TCAAATGATC TCTTTAAAAG CTTTGAAGGC 
AACCCTACAC ATAGTCAGAA GGAAGGTCTG GACCCCACAA TATATGGATA TAATTTTGAT 
CCAAAGACCT ACATCAGACA GACAAGTCAA AAGGAAGCTA GCTATTTGCC AGCTAATACA 
GAAAGAACTG AACAAAAGTC TCCAGACATT GAAAATATGC AACCAGACCT GTTTGATCCT 
TTGAACTCTG GCAGCCTAAA TCTTTGTGCA AATTTGTCCA TTTCAGGTAA ACTTGGTATC 
TCCCAGGATG ATAGTGAAAT TGCACAAGTG GAACACAGTG TGGCATCCAG AAGCTCATCA 
GACGATTGCC ATGATCGTCA GTCTGCCCCC ACTTCAGGAG TTAGGACAAT TGAAGTTAAG 
CCATGTAATA AAGACTGTTT CAGTGGAGAG AAAGTAACTG TTAAAATAGG ACCTTGGACA 
GAGCTTCAAC AAGATGAAAT ATTTGTGGAT AATTTACAAC TGCCCAACTT TGAGTCCTTA 
GACTCTAATG GTAAATCTAA ACCTATGGAA ATAACACTTG AAAAGGAAGC TTTGCAGGAA 
GCTAAGTGTC ATTCTGTCGG GGAATCGTTA GCTAAGTTAA GAAATAATCA ACCTGCTTCT 
ACAAGAGAAT ACCACCTTGT AGTGAGTGGA GACACCATTA AGTTACCAGA CATTAGTGCC 
ACGTGTGCCT CATCTCGGTT CTCAGACTCA GGTGTGGAAA GTGAGCCAAG CTCTGTTGCA 
GCACATCCAA ACCCTGACAT AGTCTTTGAA ACTGTACCAG GGCAAGGTCT TTTCATCAGT 
GAAAGGTTAT TTCCTCAGCT TTTGATGAAG CCTGACTGTA ATGTAAAGTT TTCACTAGGC 
AGTCACTGTA CTGAGAGTAC AAGTGCTTTC AGTGAGATCC AGTCGTCCTT AACCTCCATA 
AATTCTCTGC CTTCTGATGA CGAGCTGTCA CCTGATGAAA ATCCTAAGAA ATCTGCTGTA 
TCTGAATGCC ACCTAAGTGA TAGCAAAACT GTGTTAAATC TAGGAACAAC TGATATGCCA 
AAATGTGACG ATAGGAAAAA GTCAAGTATT ATTTTGCAGC AGCAGAGTGT CATATTTTCA 
GGGCATTCGG ACAATGAAAC TATAGAAATA CATTCCTTAA ATTCAAGCAC TAAAGACCCT 
TTACAATTTG TTTTTTCAGA TGAAGATACT TCTAGTGATG TGAAAAGTAG TTGCAGCTCC 
AAACCTAACT TGGACACTGT GTGTAAAGAC TCCCAGAGTC CTGATAAATC CAGTGACTCT 
GTAGGAACCA CAGTTCCGTT AAGTTCAGAA CTGGCTTGTT CAGGCACCCC TTGTGTCGTT 
TCAGGTTCCG TTTCCACCAG GGCAGATAGC AGTGAAGATA GAACTGTGAA AAGAAAAAAC 
AGTGATGCAT TAAATCCCAA ACAAATGTGT TCAGAAGCCC CTGCAGTTAC AGATGAAACT 
TACCTGGGTA CAGGTGATGC TTTTTCAGCC AGTCCTGATA TGGTAAAGCA AGGGCTTGTG 
GAAAATTATT TTGGCTGTCA AAGCAGTACG GATGTTTCTG ACACATGGGC TATTAGCTAC 
AGCCATTCAG TTAACCCTCA GAAAGAAACT TCTGGGAAAG AAATGAGTAG TCCTCAGCGG 
GATCAGGGTA AAGATGAGGA AGAGGAAGAG CAGGATCAAC AAATGGTTCA AAACGGGTAT 
TATGAGGAGG CAGATTACTC CTCTCTGGAG GCCGCGGGAG ATGCTCACTG TGCAGACGGA 
GCTGACCTGG GAGGAGAAAG ACTTGCCAAG TCTGAAAGAC TGGCCGGCGA CTATCTGAGC 
GATGGCATAA CCATGCCTAC TGTCTGTACT TCTGGCTGTC TGTCCTTCCC GTCTGCACCA 
CGAGAGTCCC CTTGTGGCGT TAAGTACTCT TCTAAGAGTA AATGTGACGC TGTTACCAAG 
CAGCCGAGCA GCGCCTCCCA CAGCTGCACC TCATCGCTGT CCTGGTACGA AAACTCGCCA 
AAGCCTCAGA TCCAAGCCTT CCTTCAGGCA AAAGATGAAT TGAAGCAACT TAAACTCCCC 
GGGTTCATGT ACAGCGATGT TCCTCTGCTG GCATCCTCAG TCCCTTATTT TTGCGTGGAG 
GAGGAGGAGG AGGACCATTA TGAAAATGGA GTGCATCTCA TTGTCTGTGT GCACGGCCTA 
GACGGTAACA GTGCAGATCT CCGATTAGTA AAAACTTACA TTGAACTTGG GCTGCCTGGG 
GAAAGAATTG ATTTTCTTAT GTCTGAAAGA AATCAGAATG ATACTTTTGC TGATTTTGAT 
AGCATGACTG ATCGCCTTTT GGATGAGATA ATACAATATA TTCAGATATA TAGTCTAACA 
GTCTCAAAAA TAAGCTTTAT TGGACATTCT TTGGGCAATT TAATAATCCG TTCAGTGCTT 
ACAAGACCAC GGTTTAAATA TTACCTCAGC AGGCTTCATA CCTTTCTTTC ACTTTCTGGA 
CCTCACCTTG GTACACTCTA CAATAGCAGT GCTCTTGTTA ATACAGGTCT GTGGTTTATG 
CAGAAATGGA AAAAATCAGG TTCTCTTTTA CAATTGACAT GTCGTGATCA TTCAGACCCT 
CGCCAAACTT TCTTATACAA GCTTAGTAAT AAAGCAGGGC TTCACTACTT CAGAAATGTT 
GTCCTAGTGG GATCTCTGCA AGATCGCTAT GTTCCTTATC ATTCTGCCCG CATTGAAATG 
TGCAAGACAG CTTTGAAGGA CAAACAGTCA GGACAGATCT ATTCAGAAAT GATCCACAAC 
TTACTTCGCC CGGTTCTGCA AAGCAAGGAC TGTAACTTGG TTCGATATAA TGTCATCAAT 
GCATTGCCCA ATACAGCTGA TTCACTCATT GGCAGAGCTG CACATATAGC TGTTCTGGAT 
TCAGAAATAT TTTTAGAGAA ATTCTTTCTG GTTGCTGCCC TCAAATATTT CCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025150728.1
CDS	378..4934
Translation	MTEVQAMVEFSVELNKFYNVDLFQRGFYQIRASMKIPPRVPHRVEASLLHAAGMTLAFPASVHDSLICSKTFQILYKNEEVVLNDVMIFKVKMLLDEKKIEETLEEMNFLLSLDLHFTDGDYSADDLNALQLISSRTLKLHFSLQRGLHHHANVMFDYFHLSVVSVTVHASLVALHQPLISFPRPVKTTWLNRNAPAQNKDSVIPTLESVVFGINYTKQLSPDGCSFVIADSFLRHAYRFHYTLCAALLLAFKGLHSYFITVTEELPSCQKLELEEMDVEARLTELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVITLHEELRLLLAQEHHTLRVRRFSEAFFCFEHPREAAIAYQELHAQSHLQMCTAIKNTSFCSSLPPLPIECSELDGDLNSLPIIFEDRYLDSVTEDLDAPWMGIQSLQRSESSRMDKCETEESTLAGLSSPELKIRPAGTSSFWSTEGEKQLTKSLKGKNEESNKSKVKVTKLMKTMKPENTKKLIKQNSKDLVVLVGYKCLKSTASNDLFKSFEGNPTHSQKEGLDPTIYGYNFDPKTYIRQTSQKEASYLPANTERTEQKSPDIENMQPDLFDPLNSGSLNLCANLSISGKLGISQDDSEIAQVEHSVASRSSSDDCHDRQSAPTSGVRTIEVKPCNKDCFSGEKVTVKIGPWTELQQDEIFVDNLQLPNFESLDSNGKSKPMEITLEKEALQEAKCHSVGESLAKLRNNQPASTREYHLVVSGDTIKLPDISATCASSRFSDSGVESEPSSVAAHPNPDIVFETVPGQGLFISERLFPQLLMKPDCNVKFSLGSHCTESTSAFSEIQSSLTSINSLPSDDELSPDENPKKSAVSECHLSDSKTVLNLGTTDMPKCDDRKKSSIILQQQSVIFSGHSDNETIEIHSLNSSTKDPLQFVFSDEDTSSDVKSSCSSKPNLDTVCKDSQSPDKSSDSVGTTVPLSSELACSGTPCVVSGSVSTRADSSEDRTVKRKNSDALNPKQMCSEAPAVTDETYLGTGDAFSASPDMVKQGLVENYFGCQSSTDVSDTWAISYSHSVNPQKETSGKEMSSPQRDQGKDEEEEEQDQQMVQNGYYEEADYSSLEAAGDAHCADGADLGGERLAKSERLAGDYLSDGITMPTVCTSGCLSFPSAPRESPCGVKYSSKSKCDAVTKQPSSASHSCTSSLSWYENSPKPQIQAFLQAKDELKQLKLPGFMYSDVPLLASSVPYFCVEEEEEDHYENGVHLIVCVHGLDGNSADLRLVKTYIELGLPGERIDFLMSERNQNDTFADFDSMTDRLLDEIIQYIQIYSLTVSKISFIGHSLGNLIIRSVLTRPRFKYYLSRLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSNKAGLHYFRNVVLVGSLQDRYVPYHSARIEMCKTALKDKQSGQIYSEMIHNLLRPVLQSKDCNLVRYNVINALPNTADSLIGRAAHIAVLDSEIFLEKFFLVAALKYFQ

Target ORF information:

RefSeq Version	XM_025294943.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis family with sequence similarity 135 member A (FAM135A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025294943.1

ORF Insert Sequence:

ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT 
CCGCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT 
TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG 
GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT 
GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA 
GATTATTCGG CAGATGATCT GAACGCCTTG CAGCTAATAA GCAGCCGCAC GCTGAAGCTG 
CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGCTAACG TCATGTTTGA TTATTTCCAC 
CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA 
AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA 
GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA 
TCACCAGATG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC 
CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT 
ACGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGAGGAAAT GGATGTAGAA 
GCTCGACTTA CTGAATTATG TGAAGAAGTT AAGAAAATAG AAAATCCTGA TGAACTGGCA 
GAACTTATAA ATATGAATCT TGCACAACTT TGCTCACTTT TAATGGCTTT ATGGGGACAA 
TTTCTGGAAG TTATAACACT ACATGAGGAA CTAAGACTAT TACTAGCACA AGAGCACCAT 
ACTCTGAGGG TGCGCAGGTT TTCTGAGGCC TTCTTTTGTT TTGAACATCC AAGAGAAGCT 
GCCATTGCAT ACCAGGAACT TCATGCTCAG AGTCATCTCC AGATGTGCAC CGCTATCAAA 
AACACTTCCT TCTGCAGCTC TCTCCCACCC CTGCCTATTG AATGTAGTGA ATTAGATGGA 
GATCTCAATT CGTTACCTAT TATCTTTGAA GATAGGTATT TAGATTCAGT TACTGAAGAC 
TTAGATGCAC CCTGGATGGG AATTCAGAGT CTTCAGAGAT CAGAGTCCAG TAGAATGGAT 
AAATGTGAGA CTGAAGAAAG CACTCTAGCT GGACTTTCCA GCCCAGAGTT GAAAATCAGA 
CCTGCTGGGA CCTCCAGTTT TTGGTCTACA GAAGGTGAAA AGCAGCTAAC AAAGTCTCTG 
AAAGGAAAGA ATGAAGAATC AAATAAATCC AAGGTTAAGG TTACTAAGCT CATGAAAACA 
ATGAAACCTG AAAACACAAA AAAATTAATA AAACAGAACT CTAAGGATTT GGTGGTTTTG 
GTAGGCTACA AATGCTTGAA AAGTACAGCA TCAAATGATC TCTTTAAAAG CTTTGAAGGC 
AACCCTACAC ATAGTCAGAA GGAAGGTCTG GACCCCACAA TATATGGATA TAATTTTGAT 
CCAAAGACCT ACATCAGACA GACAAGTCAA AAGGAAGCTA GCTATTTGCC AGCTAATACA 
GAAAGAACTG AACAAAAGTC TCCAGACATT GAAAATATGC AACCAGACCT GTTTGATCCT 
TTGAACTCTG GCAGCCTAAA TCTTTGTGCA AATTTGTCCA TTTCAGGTAA ACTTGGTATC 
TCCCAGGATG ATAGTGAAAT TGCACAAGTG GAACACAGTG TGGCATCCAG AAGCTCATCA 
GACGATTGCC ATGATCGTCA GTCTGCCCCC ACTTCAGGAG TTAGGACAAT TGAAGTTAAG 
CCATGTAATA AAGACTGTTT CAGTGGAGAG AAAGTAACTG TTAAAATAGG ACCTTGGACA 
GAGCTTCAAC AAGATGAAAT ATTTGTGGAT AATTTACAAC TGCCCAACTT TGAGTCCTTA 
GACTCTAATG GTAAATCTAA ACCTATGGAA ATAACACTTG AAAAGGAAGC TTTGCAGGAA 
GCTAAGTGTC ATTCTGTCGG GGAATCGTTA GCTAAGTTAA GAAATAATCA ACCTGCTTCT 
ACAAGAGAAT ACCACCTTGT AGTGAGTGGA GACACCATTA AGTTACCAGA CATTAGTGCC 
ACGTGTGCCT CATCTCGGTT CTCAGACTCA GGTGTGGAAA GTGAGCCAAG CTCTGTTGCA 
GCACATCCAA ACCCTGACAT AGTCTTTGAA ACTGTACCAG GGCAAGGTCT TTTCATCAGT 
GAAAGGTTAT TTCCTCAGCT TTTGATGAAG CCTGACTGTA ATGTAAAGTT TTCACTAGGC 
AGTCACTGTA CTGAGAGTAC AAGTGCTTTC AGTGAGATCC AGTCGTCCTT AACCTCCATA 
AATTCTCTGC CTTCTGATGA CGAGCTGTCA CCTGATGAAA ATCCTAAGAA ATCTGCTGTA 
TCTGAATGCC ACCTAAGTGA TAGCAAAACT GTGTTAAATC TAGGAACAAC TGATATGCCA 
AAATGTGACG ATAGGAAAAA GTCAAGTATT ATTTTGCAGC AGCAGAGTGT CATATTTTCA 
GGGCATTCGG ACAATGAAAC TATAGAAATA CATTCCTTAA ATTCAAGCAC TAAAGACCCT 
TTACAATTTG TTTTTTCAGA TGAAGATACT TCTAGTGATG TGAAAAGTAG TTGCAGCTCC 
AAACCTAACT TGGACACTGT GTGTAAAGAC TCCCAGAGTC CTGATAAATC CAGTGACTCT 
GTAGGAACCA CAGTTCCGTT AAGTTCAGAA CTGGCTTGTT CAGGCACCCC TTGTGTCGTT 
TCAGGTTCCG TTTCCACCAG GGCAGATAGC AGTGAAGATA GAACTGTGAA AAGAAAAAAC 
AGTGATGCAT TAAATCCCAA ACAAATGTGT TCAGAAGCCC CTGCAGTTAC AGATGAAACT 
TACCTGGGTA CAGGTGATGC TTTTTCAGCC AGTCCTGATA TGGTAAAGCA AGGGCTTGTG 
GAAAATTATT TTGGCTGTCA AAGCAGTACG GATGTTTCTG ACACATGGGC TATTAGCTAC 
AGCCATTCAG TTAACCCTCA GAAAGAAACT TCTGGGAAAG AAATGAGTAG TCCTCAGCGG 
GATCAGGGTA AAGATGAGGA AGAGGAAGAG CAGGATCAAC AAATGGTTCA AAACGGGTAT 
TATGAGGAGG CAGATTACTC CTCTCTGGAG GCCGCGGGAG ATGCTCACTG TGCAGACGGA 
GCTGACCTGG GAGGAGAAAG ACTTGCCAAG TCTGAAAGAC TGGCCGGCGA CTATCTGAGC 
GATGGCATAA CCATGCCTAC TGTCTGTACT TCTGGCTGTC TGTCCTTCCC GTCTGCACCA 
CGAGAGTCCC CTTGTGGCGT TAAGTACTCT TCTAAGAGTA AATGTGACGC TGTTACCAAG 
CAGCCGAGCA GCGCCTCCCA CAGCTGCACC TCATCGCTGT CCTGGTACGA AAACTCGCCA 
AAGCCTCAGA TCCAAGCCTT CCTTCAGGCA AAAGATGAAT TGAAGCAACT TAAACTCCCC 
GGGTTCATGT ACAGCGATGT TCCTCTGCTG GCATCCTCAG TCCCTTATTT TTGCGTGGAG 
GAGGAGGAGG AGGACCATTA TGAAAATGGA GTGCATCTCA TTGTCTGTGT GCACGGCCTA 
GACGGTAACA GTGCAGATCT CCGATTAGTA AAAACTTACA TTGAACTTGG GCTGCCTGGG 
GAAAGAATTG ATTTTCTTAT GTCTGAAAGA AATCAGAATG ATACTTTTGC TGATTTTGAT 
AGCATGACTG ATCGCCTTTT GGATGAGATA ATACAATATA TTCAGATATA TAGTCTAACA 
GTCTCAAAAA TAAGCTTTAT TGGACATTCT TTGGGCAATT TAATAATCCG TTCAGTGCTT 
ACAAGACCAC GGTTTAAATA TTACCTCAGC AGGCTTCATA CCTTTCTTTC ACTTTCTGGA 
CCTCACCTTG GTACACTCTA CAATAGCAGT GCTCTTGTTA ATACAGGTCT GTGGTTTATG 
CAGAAATGGA AAAAATCAGG TTCTCTTTTA CAATTGACAT GTCGTGATCA TTCAGACCCT 
CGCCAAACTT TCTTATACAA GCTTAGTAAT AAAGCAGGGC TTCACTACTT CAGAAATGTT 
GTCCTAGTGG GATCTCTGCA AGATCGCTAT GTTCCTTATC ATTCTGCCCG CATTGAAATG 
TGCAAGACAG CTTTGAAGGA CAAACAGTCA GGACAGATCT ATTCAGAAAT GATCCACAAC 
TTACTTCGCC CGGTTCTGCA AAGCAAGGAC TGTAACTTGG TTCGATATAA TGTCATCAAT 
GCATTGCCCA ATACAGCTGA TTCACTCATT GGCAGAGCTG CACATATAGC TGTTCTGGAT 
TCAGAAATAT TTTTAGAGAA ATTCTTTCTG GTTGCTGCCC TCAAATATTT CCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	FAM135A
Entrez Gene ID	102392058
Full Name	family with sequence similarity 135 member A
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

FAM135A cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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