LOC100822255 cDNA ORF clone, Brachypodium distachyon(stiff brome)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100822255
Entrez Gene ID	100822255
Full Name	uncharacterized LOC100822255
Gene Type	protein-coding
Organism	Brachypodium distachyon(stiff brome)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003574121.3	XP_003574169.1	uncharacterized protein LOC100822255
XM_003574121.4	XP_003574169.1	protein GRAVITROPIC IN THE LIGHT 1

Pathways from BioSystems
Gene Ontology

The following LOC100822255 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100822255 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBr110854	XM_003574121.4 Latest version!	Brachypodium distachyon protein GRAVITROPIC IN THE LIGHT 1 (LOC100822255), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
View Old Accession Versions >>
OBr110854	XM_003574121.3	Brachypodium distachyon uncharacterized LOC100822255 (LOC100822255), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100822255 ( XM_003574121.3 ) cDNA ORF clone, Brachypodium distachyon(stiff brome) -> XP_003574169.1 Brachypodium distachyon uncharacterized LOC100822255 (LOC100822255), mRNA.

Price & Availability↑

CloneID	OBr110854
Clone ID Related Accession (Same CDS sequence)	XM_003574121.3 , XM_003574121.4
Accession Version	XM_003574121.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-07
Organism	Brachypodium distachyon(stiff brome)
Product	uncharacterized protein LOC100822255
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014576700.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 8, 2015 this sequence version replaced XM_003574121.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Brachypodium distachyon Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCAGA AGTTCGCTCT CGCGTTCAAG ACCAAGACCA TCGAGTTCTT CGCCGAGGAG 
GAGGAGGACG AGTATGCGGC GGCGGGGGAG GGCGGGGTCC TCGCTGGCCA ACGGGTGGTT 
GTGCTGAAGC CCGACCCGCA GAACCCTAGC CCTGTCGTCG GCGGGGAAGG GGATGGGGAG 
GGCGTGCTTG CGTCCAGCGA GGATGCGGCC GTAGAGGCGG CGCTCGCGAC GGCGTCGTTG 
TTCCAGGCGG CGTACCTGCA CCTGCAAGCG GCGCACTCGC CGTTCCTGCC GGACGCCGCG 
GCGGCGGCGG ACGCCCTTGC TGTTTCGCAC CTCCGGAGGC TGTCCGAGCT AAAGCGGCTC 
GCGTCGGGCG CGCCGGCAGA GGGGGATGGG CCCCTCACGG CCCACCTCGA GGACCAGGTG 
CGCGAGAACC AGGCGCTGCT GCGGTCGTTC GACGCCGTGG TGAATCGCCT CCAGGCCGCG 
CTCGACGCCA AGGACACCGC GGCCGCCGCT CTTCGCTGGG AGCACGCGGC GCTGGCGGAC 
GGCAACGCGC GCCTCGCTGG GCGCCTCGAC CGCGCCCTCG CGCCACAGCC GGGCGCAGGT 
GGCGGCGACG CCTTGGGTGC CATGCTGTCC GCGAGCGTCT TCGACTCCGT CCTACGCGAT 
GCTCTCCGCG TCGCCCACCG CTTCACCCGT GCGCTCGCCG AGCTCCTCAG ATGTGCTGGA 
TGGGACCTGA CTAACGCCGC AGCAGCTGCC TACCCTGGTA TTGCCTACTC CAAGCATGGC 
CATTGCCGCT ACGCCCTTCT GTCCCGTGTC TGCCTGTCCA TGTTTGACGG CTTCGACTCC 
TACCAATTCG GTGCCGCAAG TGATGATGGT GGAGCCCTTG AAGGGATTGA GCTCGCAATT 
CGCAGGAATG AATCATTGCA GCAATTCATT GAGCACTCTG ATACAGACCC CATCGAGCTC 
ATGCATTCAA GCCCTGACTG TGAATTCGCC CAATTTTGCG ATCGGAAGTA CAAACAGCTT 
ATCCATCCAG GCATTGAGTC TTCATTGTTT GGGAATTCAG AATGCGGGGC ATTGCCAGTG 
TTGGCTGCAG CAGGTCCTCT CTATGAGCTG TTCATCACAA TGGCAAGCTC AATATGGACA 
CTCCACAGGT TGGCCTGGGC GTATGACCCA GCAGTCGGCA TATTCCAGGT TAGCCGAGGC 
ACAGAGTACT CGTCGGTTTA CATGGAGAGC ATCGTGCGTC CGAATACATT TTCAGCAAGC 
AAAGAGGTCG GGAAGACGGT GCGTCCAAAG GTCGGGTTCA CCGTGGTGCC GGGCTTCCGG 
CTTGGCGGGA CGGTGCTCCA ATGTCGGGTG TACTTAGAGC CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003574169.1
CDS	301..1665
Translation	MLQKFALAFKTKTIEFFAEEEEDEYAAAGEGGVLAGQRVVVLKPDPQNPSPVVGGEGDGEGVLASSEDAAVEAALATASLFQAAYLHLQAAHSPFLPDAAAAADALAVSHLRRLSELKRLASGAPAEGDGPLTAHLEDQVRENQALLRSFDAVVNRLQAALDAKDTAAAALRWEHAALADGNARLAGRLDRALAPQPGAGGGDALGAMLSASVFDSVLRDALRVAHRFTRALAELLRCAGWDLTNAAAAAYPGIAYSKHGHCRYALLSRVCLSMFDGFDSYQFGAASDDGGALEGIELAIRRNESLQQFIEHSDTDPIELMHSSPDCEFAQFCDRKYKQLIHPGIESSLFGNSECGALPVLAAAGPLYELFITMASSIWTLHRLAWAYDPAVGIFQVSRGTEYSSVYMESIVRPNTFSASKEVGKTVRPKVGFTVVPGFRLGGTVLQCRVYLEP

Target ORF information:

RefSeq Version	XM_003574121.3
Organism	Brachypodium distachyon(stiff brome)
Definition	Brachypodium distachyon uncharacterized LOC100822255 (LOC100822255), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003574121.3

ORF Insert Sequence:

ATGCTCCAGA AGTTCGCTCT CGCGTTCAAG ACCAAGACCA TCGAGTTCTT CGCCGAGGAG 
GAGGAGGACG AGTATGCGGC GGCGGGGGAG GGCGGGGTCC TCGCTGGCCA ACGGGTGGTT 
GTGCTGAAGC CCGACCCGCA GAACCCTAGC CCTGTCGTCG GCGGGGAAGG GGATGGGGAG 
GGCGTGCTTG CGTCCAGCGA GGATGCGGCC GTAGAGGCGG CGCTCGCGAC GGCGTCGTTG 
TTCCAGGCGG CGTACCTGCA CCTGCAAGCG GCGCACTCGC CGTTCCTGCC GGACGCCGCG 
GCGGCGGCGG ACGCCCTTGC TGTTTCGCAC CTCCGGAGGC TGTCCGAGCT AAAGCGGCTC 
GCGTCGGGCG CGCCGGCAGA GGGGGATGGG CCCCTCACGG CCCACCTCGA GGACCAGGTG 
CGCGAGAACC AGGCGCTGCT GCGGTCGTTC GACGCCGTGG TGAATCGCCT CCAGGCCGCG 
CTCGACGCCA AGGACACCGC GGCCGCCGCT CTTCGCTGGG AGCACGCGGC GCTGGCGGAC 
GGCAACGCGC GCCTCGCTGG GCGCCTCGAC CGCGCCCTCG CGCCACAGCC GGGCGCAGGT 
GGCGGCGACG CCTTGGGTGC CATGCTGTCC GCGAGCGTCT TCGACTCCGT CCTACGCGAT 
GCTCTCCGCG TCGCCCACCG CTTCACCCGT GCGCTCGCCG AGCTCCTCAG ATGTGCTGGA 
TGGGACCTGA CTAACGCCGC AGCAGCTGCC TACCCTGGTA TTGCCTACTC CAAGCATGGC 
CATTGCCGCT ACGCCCTTCT GTCCCGTGTC TGCCTGTCCA TGTTTGACGG CTTCGACTCC 
TACCAATTCG GTGCCGCAAG TGATGATGGT GGAGCCCTTG AAGGGATTGA GCTCGCAATT 
CGCAGGAATG AATCATTGCA GCAATTCATT GAGCACTCTG ATACAGACCC CATCGAGCTC 
ATGCATTCAA GCCCTGACTG TGAATTCGCC CAATTTTGCG ATCGGAAGTA CAAACAGCTT 
ATCCATCCAG GCATTGAGTC TTCATTGTTT GGGAATTCAG AATGCGGGGC ATTGCCAGTG 
TTGGCTGCAG CAGGTCCTCT CTATGAGCTG TTCATCACAA TGGCAAGCTC AATATGGACA 
CTCCACAGGT TGGCCTGGGC GTATGACCCA GCAGTCGGCA TATTCCAGGT TAGCCGAGGC 
ACAGAGTACT CGTCGGTTTA CATGGAGAGC ATCGTGCGTC CGAATACATT TTCAGCAAGC 
AAAGAGGTCG GGAAGACGGT GCGTCCAAAG GTCGGGTTCA CCGTGGTGCC GGGCTTCCGG 
CTTGGCGGGA CGGTGCTCCA ATGTCGGGTG TACTTAGAGC CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100822255 ( XM_003574121.4 ) cDNA ORF clone, Brachypodium distachyon(stiff brome) -> XP_003574169.1 Brachypodium distachyon protein GRAVITROPIC IN THE LIGHT 1 (LOC100822255), mRNA.

Price & Availability↑

CloneID	OBr110854
Clone ID Related Accession (Same CDS sequence)	XM_003574121.3 , XM_003574121.4
Accession Version	XM_003574121.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-26
Organism	Brachypodium distachyon(stiff brome)
Product	protein GRAVITROPIC IN THE LIGHT 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016133.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 27, 2018 this sequence version replaced XM_003574121.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Brachypodium distachyon Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCAGA AGTTCGCTCT CGCGTTCAAG ACCAAGACCA TCGAGTTCTT CGCCGAGGAG 
GAGGAGGACG AGTATGCGGC GGCGGGGGAG GGCGGGGTCC TCGCTGGCCA ACGGGTGGTT 
GTGCTGAAGC CCGACCCGCA GAACCCTAGC CCTGTCGTCG GCGGGGAAGG GGATGGGGAG 
GGCGTGCTTG CGTCCAGCGA GGATGCGGCC GTAGAGGCGG CGCTCGCGAC GGCGTCGTTG 
TTCCAGGCGG CGTACCTGCA CCTGCAAGCG GCGCACTCGC CGTTCCTGCC GGACGCCGCG 
GCGGCGGCGG ACGCCCTTGC TGTTTCGCAC CTCCGGAGGC TGTCCGAGCT AAAGCGGCTC 
GCGTCGGGCG CGCCGGCAGA GGGGGATGGG CCCCTCACGG CCCACCTCGA GGACCAGGTG 
CGCGAGAACC AGGCGCTGCT GCGGTCGTTC GACGCCGTGG TGAATCGCCT CCAGGCCGCG 
CTCGACGCCA AGGACACCGC GGCCGCCGCT CTTCGCTGGG AGCACGCGGC GCTGGCGGAC 
GGCAACGCGC GCCTCGCTGG GCGCCTCGAC CGCGCCCTCG CGCCACAGCC GGGCGCAGGT 
GGCGGCGACG CCTTGGGTGC CATGCTGTCC GCGAGCGTCT TCGACTCCGT CCTACGCGAT 
GCTCTCCGCG TCGCCCACCG CTTCACCCGT GCGCTCGCCG AGCTCCTCAG ATGTGCTGGA 
TGGGACCTGA CTAACGCCGC AGCAGCTGCC TACCCTGGTA TTGCCTACTC CAAGCATGGC 
CATTGCCGCT ACGCCCTTCT GTCCCGTGTC TGCCTGTCCA TGTTTGACGG CTTCGACTCC 
TACCAATTCG GTGCCGCAAG TGATGATGGT GGAGCCCTTG AAGGGATTGA GCTCGCAATT 
CGCAGGAATG AATCATTGCA GCAATTCATT GAGCACTCTG ATACAGACCC CATCGAGCTC 
ATGCATTCAA GCCCTGACTG TGAATTCGCC CAATTTTGCG ATCGGAAGTA CAAACAGCTT 
ATCCATCCAG GCATTGAGTC TTCATTGTTT GGGAATTCAG AATGCGGGGC ATTGCCAGTG 
TTGGCTGCAG CAGGTCCTCT CTATGAGCTG TTCATCACAA TGGCAAGCTC AATATGGACA 
CTCCACAGGT TGGCCTGGGC GTATGACCCA GCAGTCGGCA TATTCCAGGT TAGCCGAGGC 
ACAGAGTACT CGTCGGTTTA CATGGAGAGC ATCGTGCGTC CGAATACATT TTCAGCAAGC 
AAAGAGGTCG GGAAGACGGT GCGTCCAAAG GTCGGGTTCA CCGTGGTGCC GGGCTTCCGG 
CTTGGCGGGA CGGTGCTCCA ATGTCGGGTG TACTTAGAGC CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003574169.1
CDS	305..1669
Translation	MLQKFALAFKTKTIEFFAEEEEDEYAAAGEGGVLAGQRVVVLKPDPQNPSPVVGGEGDGEGVLASSEDAAVEAALATASLFQAAYLHLQAAHSPFLPDAAAAADALAVSHLRRLSELKRLASGAPAEGDGPLTAHLEDQVRENQALLRSFDAVVNRLQAALDAKDTAAAALRWEHAALADGNARLAGRLDRALAPQPGAGGGDALGAMLSASVFDSVLRDALRVAHRFTRALAELLRCAGWDLTNAAAAAYPGIAYSKHGHCRYALLSRVCLSMFDGFDSYQFGAASDDGGALEGIELAIRRNESLQQFIEHSDTDPIELMHSSPDCEFAQFCDRKYKQLIHPGIESSLFGNSECGALPVLAAAGPLYELFITMASSIWTLHRLAWAYDPAVGIFQVSRGTEYSSVYMESIVRPNTFSASKEVGKTVRPKVGFTVVPGFRLGGTVLQCRVYLEP

Target ORF information:

RefSeq Version	XM_003574121.4
Organism	Brachypodium distachyon(stiff brome)
Definition	Brachypodium distachyon protein GRAVITROPIC IN THE LIGHT 1 (LOC100822255), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003574121.4

ORF Insert Sequence:

ATGCTCCAGA AGTTCGCTCT CGCGTTCAAG ACCAAGACCA TCGAGTTCTT CGCCGAGGAG 
GAGGAGGACG AGTATGCGGC GGCGGGGGAG GGCGGGGTCC TCGCTGGCCA ACGGGTGGTT 
GTGCTGAAGC CCGACCCGCA GAACCCTAGC CCTGTCGTCG GCGGGGAAGG GGATGGGGAG 
GGCGTGCTTG CGTCCAGCGA GGATGCGGCC GTAGAGGCGG CGCTCGCGAC GGCGTCGTTG 
TTCCAGGCGG CGTACCTGCA CCTGCAAGCG GCGCACTCGC CGTTCCTGCC GGACGCCGCG 
GCGGCGGCGG ACGCCCTTGC TGTTTCGCAC CTCCGGAGGC TGTCCGAGCT AAAGCGGCTC 
GCGTCGGGCG CGCCGGCAGA GGGGGATGGG CCCCTCACGG CCCACCTCGA GGACCAGGTG 
CGCGAGAACC AGGCGCTGCT GCGGTCGTTC GACGCCGTGG TGAATCGCCT CCAGGCCGCG 
CTCGACGCCA AGGACACCGC GGCCGCCGCT CTTCGCTGGG AGCACGCGGC GCTGGCGGAC 
GGCAACGCGC GCCTCGCTGG GCGCCTCGAC CGCGCCCTCG CGCCACAGCC GGGCGCAGGT 
GGCGGCGACG CCTTGGGTGC CATGCTGTCC GCGAGCGTCT TCGACTCCGT CCTACGCGAT 
GCTCTCCGCG TCGCCCACCG CTTCACCCGT GCGCTCGCCG AGCTCCTCAG ATGTGCTGGA 
TGGGACCTGA CTAACGCCGC AGCAGCTGCC TACCCTGGTA TTGCCTACTC CAAGCATGGC 
CATTGCCGCT ACGCCCTTCT GTCCCGTGTC TGCCTGTCCA TGTTTGACGG CTTCGACTCC 
TACCAATTCG GTGCCGCAAG TGATGATGGT GGAGCCCTTG AAGGGATTGA GCTCGCAATT 
CGCAGGAATG AATCATTGCA GCAATTCATT GAGCACTCTG ATACAGACCC CATCGAGCTC 
ATGCATTCAA GCCCTGACTG TGAATTCGCC CAATTTTGCG ATCGGAAGTA CAAACAGCTT 
ATCCATCCAG GCATTGAGTC TTCATTGTTT GGGAATTCAG AATGCGGGGC ATTGCCAGTG 
TTGGCTGCAG CAGGTCCTCT CTATGAGCTG TTCATCACAA TGGCAAGCTC AATATGGACA 
CTCCACAGGT TGGCCTGGGC GTATGACCCA GCAGTCGGCA TATTCCAGGT TAGCCGAGGC 
ACAGAGTACT CGTCGGTTTA CATGGAGAGC ATCGTGCGTC CGAATACATT TTCAGCAAGC 
AAAGAGGTCG GGAAGACGGT GCGTCCAAAG GTCGGGTTCA CCGTGGTGCC GGGCTTCCGG 
CTTGGCGGGA CGGTGCTCCA ATGTCGGGTG TACTTAGAGC CCTAG