MRPL4 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MRPL4
Entrez Gene ID	507154
Full Name	mitochondrial ribosomal protein L4
General protein information	Preferred Names mitochondrial ribosomal protein L4 Names 39S ribosomal protein L4, mitochondrial L4mt MRP-L4
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001037450.2	NP_001032527.1	39S ribosomal protein L4, mitochondrial

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	AT2G20060	NP_565463.1
Pan troglodytes (chimpanzee)	MRPL4	XP_001164062.2
Canis lupus familiaris (dog)	MRPL4	XP_854013.1
Rattus norvegicus (Norway rat)	Mrpl4	NP_001102224.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007937	XP_317543.4
Homo sapiens (human)	MRPL4	NP_666499.1
Mus musculus (house mouse)	Mrpl4	NP_075656.2
Drosophila melanogaster (fruit fly)	mRpL4	NP_524939.1
Caenorhabditis elegans (roundworm)	mrpl-4	NP_505181.1
Xenopus tropicalis (tropical clawed frog)	mrpl4	NP_001017222.1
Bos taurus (cattle)	MRPL4	NP_001032527.1
Danio rerio (zebrafish)	zgmrpl4	NP_001018572.1
Oryza sativa (rice)	Os11g0585700	NP_001068165.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

The large subunit of the mammalian mitochondrial ribosome. Analysis of the complement of ribosomal proteins present.
Koc EC, Burkhart W, Blackburn K, Moyer MB, Schlatzer DM, Moseley A, Spremulli LL
The Journal of biological chemistry276(47)43958-69(2001 Nov)

Structural compensation for the deficit of rRNA with proteins in the mammalian mitochondrial ribosome. Systematic analysis of protein components of the large ribosomal subunit from mammalian mitochondria.
Suzuki T, Terasaki M, Takemoto-Hori C, Hanada T, Ueda T, Wada A, Watanabe K
The Journal of biological chemistry276(24)21724-36(2001 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MRPL4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MRPL4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa00996	NM_001037450.2 Latest version!	Bos taurus mitochondrial ribosomal protein L4 (MRPL4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MRPL4 ( NM_001037450.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001032527.1 Bos taurus mitochondrial ribosomal protein L4 (MRPL4), mRNA.

Price & Availability↑

CloneID	OBa00996
Clone ID Related Accession (Same CDS sequence)	NM_001037450.2
Accession Version	NM_001037450.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Bos taurus(cattle)
Product	39S ribosomal protein L4, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC108102.1. On Aug 31, 2012 this sequence version replaced NM_001037450.1. ##Evidence-Data-START## Transcript exon combination :: BC108102.1, DV935170.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGCAGCAGC TAGTCCGGGC CGGGGCCCGG GCCTGGCTTC GGCCCAGGGG CTGCCGGGGC 
CTGAGCGCGC TGACAGAAGA GGCAGTGCAG TCGGCGGAGA AACCAGAACC CTTGGCGAAC 
GCAGGTCCCC AGGCACCCGT ACTGCGCAGG TGCGAGCTCC CGGTACCCCT GCACCGGCGT 
CCGGTGCAGG CCTGGGTTGA ATCTCTGCGG GGCTATGAGC AGGAGCGCGT GGGTCTGACC 
GAACTGCACC CAGACGTTTT CTCTACGGCG CCCAGGCTGG ATATCCTGCA CCAGGTTGCC 
ATCTGGCAGA AGAACTTCAA GAGAATTAGC TACGCCAAGA CCAAGACCAG GGCTGAGGTG 
CGGGGAGGTG GCCGGAAGCC CTGGCAGCAA AAAGGCAGTG GGCGCGCCAG ACACGGCAGC 
ATCCGCTCCC CAATCTGGCG AGGAGGAGGT GTTGCCCACG GCCCCCGGGG CCCCACGAGC 
TACTACTACA TGCTGCCCAT GAAAGTGCGG GTGCAAGGCC TCAAGGTGGC GCTGACCGTC 
AAGCTGGCCC AGGATGACCT GCACATCGTG GATTCCCTGG AGTTGCCCAC CACAGACCCC 
CAGTACCTGA TGGAGCTGGC CCGCTACCGC CGCTGGGGCG ACTCTGTCCT TTTTGTAGAC 
TTAGAACACG AGGACATGCC CCAGAACGTC GTAGCAGCCA CATCTGGGCT CAAGACCTTC 
AACCTGATCC CGGCTATCGG CCTGAACGTG CACAGCATGC TCAAACACCA GACCCTGGTC 
CTGACCCTGC CCACCGTCGC CTTCCTGGAA GAGAAGCTGC TCTGGCATGA CTCACGTTAC 
ACGCCCCTCT ACCCCTTCCG CCTGCCCTAC CGAGACTTCC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001032527.1
CDS	44..928
Translation	MQQLVRAGARAWLRPRGCRGLSALTEEAVQSAEKPEPLANAGPQAPVLRRCELPVPLHRRPVQAWVESLRGYEQERVGLTELHPDVFSTAPRLDILHQVAIWQKNFKRISYAKTKTRAEVRGGGRKPWQQKGSGRARHGSIRSPIWRGGGVAHGPRGPTSYYYMLPMKVRVQGLKVALTVKLAQDDLHIVDSLELPTTDPQYLMELARYRRWGDSVLFVDLEHEDMPQNVVAATSGLKTFNLIPAIGLNVHSMLKHQTLVLTLPTVAFLEEKLLWHDSRYTPLYPFRLPYRDFP

Target ORF information:

RefSeq Version	NM_001037450.2
Organism	Bos taurus(cattle)
Definition	Bos taurus mitochondrial ribosomal protein L4 (MRPL4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001037450.2

ORF Insert Sequence:

ATGCAGCAGC TAGTCCGGGC CGGGGCCCGG GCCTGGCTTC GGCCCAGGGG CTGCCGGGGC 
CTGAGCGCGC TGACAGAAGA GGCAGTGCAG TCGGCGGAGA AACCAGAACC CTTGGCGAAC 
GCAGGTCCCC AGGCACCCGT ACTGCGCAGG TGCGAGCTCC CGGTACCCCT GCACCGGCGT 
CCGGTGCAGG CCTGGGTTGA ATCTCTGCGG GGCTATGAGC AGGAGCGCGT GGGTCTGACC 
GAACTGCACC CAGACGTTTT CTCTACGGCG CCCAGGCTGG ATATCCTGCA CCAGGTTGCC 
ATCTGGCAGA AGAACTTCAA GAGAATTAGC TACGCCAAGA CCAAGACCAG GGCTGAGGTG 
CGGGGAGGTG GCCGGAAGCC CTGGCAGCAA AAAGGCAGTG GGCGCGCCAG ACACGGCAGC 
ATCCGCTCCC CAATCTGGCG AGGAGGAGGT GTTGCCCACG GCCCCCGGGG CCCCACGAGC 
TACTACTACA TGCTGCCCAT GAAAGTGCGG GTGCAAGGCC TCAAGGTGGC GCTGACCGTC 
AAGCTGGCCC AGGATGACCT GCACATCGTG GATTCCCTGG AGTTGCCCAC CACAGACCCC 
CAGTACCTGA TGGAGCTGGC CCGCTACCGC CGCTGGGGCG ACTCTGTCCT TTTTGTAGAC 
TTAGAACACG AGGACATGCC CCAGAACGTC GTAGCAGCCA CATCTGGGCT CAAGACCTTC 
AACCTGATCC CGGCTATCGG CCTGAACGTG CACAGCATGC TCAAACACCA GACCCTGGTC 
CTGACCCTGC CCACCGTCGC CTTCCTGGAA GAGAAGCTGC TCTGGCATGA CTCACGTTAC 
ACGCCCCTCT ACCCCTTCCG CCTGCCCTAC CGAGACTTCC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

The large subunit of the mammalian mitochondrial ribosome. Analysis of the complement of ribosomal proteins present.
The Journal of biological chemistry276(47)43958-69(2001 Nov)
Koc EC,Burkhart W,Blackburn K,Moyer MB,Schlatzer DM,Moseley A,Spremulli LL

Structural compensation for the deficit of rRNA with proteins in the mammalian mitochondrial ribosome. Systematic analysis of protein components of the large ribosomal subunit from mammalian mitochondria.
The Journal of biological chemistry276(24)21724-36(2001 Jun)
Suzuki T,Terasaki M,Takemoto-Hori C,Hanada T,Ueda T,Wada A,Watanabe K