CRYBB2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	CRYBB2
Entrez Gene ID	287011
Full Name	crystallin beta B2
General protein information	Preferred Names crystallin beta B2 Names beta-crystallin B2 beta-B2 crystallin beta-crystallin Bp
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024977034.1	XP_024832802.1	beta-crystallin B2 isoform X1
NM_174807.1	NP_777232.1	beta-crystallin B2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CRYBB2	NP_777232.1
Danio rerio (zebrafish)	crybb2	NP_001018138.1
Homo sapiens (human)	CRYBB2	NP_000487.1
Canis lupus familiaris (dog)	CRYBB2	NP_001041578.1
Mus musculus (house mouse)	Crybb2	NP_031799.1
Rattus norvegicus (Norway rat)	Crybb2	NP_037069.1
Gallus gallus (chicken)	CRYBB2	NP_990506.2
Pan troglodytes (chimpanzee)	CRYBB2	XP_003317196.1
	CRYBB2	NP_001116366.1
Xenopus tropicalis (tropical clawed frog)	crybb2	NP_001238879.1

Gene Ontology
Bibliography

Related articles in PubMed

Detection and structural features of the betaB2-B3-crystallin heterodimer by radical probe mass spectrometry (RP-MS).
Diemer H, Atmanene C, Sanglier S, Morrissey B, Van Dorsselaer A, Downard KM
Journal of mass spectrometry : JMS44(5)803-12(2009 May)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Identification of the primary targets of carbamylation in bovine lens proteins by mass spectrometry.
Zhang J, Yan H, Harding JJ, Liu ZX, Wang X, Ruan YS
Current eye research33(11)963-76(2008 Nov)

Association of partially folded lens betaB2-crystallins with the alpha-crystallin molecular chaperone.
Evans P, Slingsby C, Wallace BA
The Biochemical journal409(3)691-9(2008 Feb)

Calcium-binding to lens betaB2- and betaA3-crystallins suggests that all beta-crystallins are calcium-binding proteins.
Jobby MK, Sharma Y
The FEBS journal274(16)4135-47(2007 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

detection & sructure of the betaB2-B3-crystallin heterodimer; results suggest the betaB2 subunit adopts a more compacted form than in its monomeric form in order for much of its structure to be enveloped by the betaB3 subunit within the heterodimer
Title: Detection and structural features of the betaB2-B3-crystallin heterodimer by radical probe mass spectrometry (RP-MS).

Mass spectrometry analysis and a database search identified carbamylated proteins originating from alphaA-crystallin, betaB2- and gammaS-(betaS)-crystallins.
Title: Identification of the primary targets of carbamylation in bovine lens proteins by mass spectrometry.

combined with previously reported observations of alpha-crystallin quaternary structure have led us to propose a structural model of how activated alpha-crystallin chaperones unfolded betaB2-crystallin
Title: Association of partially folded lens betaB2-crystallins with the alpha-crystallin molecular chaperone.

Results show that both betaB2- and betaA3-crystallin bind calcium with moderate affinity.
Title: Calcium-binding to lens betaB2- and betaA3-crystallins suggests that all beta-crystallins are calcium-binding proteins.

The following CRYBB2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CRYBB2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa42328	NM_174807.1 Latest version!	Bos taurus crystallin beta B2 (CRYBB2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa224160	XM_024977034.1 Latest version!	Bos taurus crystallin beta B2 (CRYBB2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CRYBB2 ( NM_174807.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_777232.1 Bos taurus crystallin beta B2 (CRYBB2), mRNA.

Price & Availability↑

CloneID	OBa42328
Clone ID Related Accession (Same CDS sequence)	NM_174807.1
Accession Version	NM_174807.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Bos taurus(cattle)
Product	beta-crystallin B2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M22466.1. ##Evidence-Data-START## Transcript exon combination :: M22466.1, EG707480.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145425, SAMN03145430 [ECO:0000348] ##Evidence-Data-END##

ATGGCCTCAG ATCACCAGAC CCAAGCGGGC AAGCCGCAGC CCCTCAACCC CAAGATCATC 
ATCTTTGAGC AGGAGAACTT CCAGGGTCAC TCGCATGAGC TCAACGGGCC CTGCCCCAAC 
CTGAAGGAGA CTGGCGTGGA GAAGGCGGGC TCCGTCCTGG TGCAGGCTGG ACCTTGGGTG 
GGCTATGAGC AGGCCAACTG CAAAGGGGAG CAGTTCGTGT TTGAGAAGGG TGAGTACCCC 
CGCTGGGACT CGTGGACCAG CAGTCGGAGG ACGGACTCGC TCAGCTCCCT GAGGCCCATC 
AAAGTGGACA GCCAGGAGCA TAAGATCACC CTGTATGAGA ACCCCAACTT CACGGGGAAG 
AAGATGGAGG TGATAGACGA TGACGTGCCC AGCTTCCACG CCCACGGCTA CCAGGAGAAG 
GTGTCTTCCG TGCGTGTGCA GAGCGGCACG TGGGTTGGCT ACCAGTACCC CGGCTACCGC 
GGGCTGCAGT ACCTGCTGGA GAAGGGCGAT TACAAGGACA GCGGTGACTT TGGGGCCCCC 
CAGCCCCAGG TGCAGTCCGT GCGCCGCATC CGGGACATGC AGTGGCACCA GCGGGGCGCC 
TTCCACCCCT CCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777232.1
CDS	56..673
Translation	MASDHQTQAGKPQPLNPKIIIFEQENFQGHSHELNGPCPNLKETGVEKAGSVLVQAGPWVGYEQANCKGEQFVFEKGEYPRWDSWTSSRRTDSLSSLRPIKVDSQEHKITLYENPNFTGKKMEVIDDDVPSFHAHGYQEKVSSVRVQSGTWVGYQYPGYRGLQYLLEKGDYKDSGDFGAPQPQVQSVRRIRDMQWHQRGAFHPSS

Target ORF information:

RefSeq Version	NM_174807.1
Organism	Bos taurus(cattle)
Definition	Bos taurus crystallin beta B2 (CRYBB2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174807.1

ORF Insert Sequence:

ATGGCCTCAG ATCACCAGAC CCAAGCGGGC AAGCCGCAGC CCCTCAACCC CAAGATCATC 
ATCTTTGAGC AGGAGAACTT CCAGGGTCAC TCGCATGAGC TCAACGGGCC CTGCCCCAAC 
CTGAAGGAGA CTGGCGTGGA GAAGGCGGGC TCCGTCCTGG TGCAGGCTGG ACCTTGGGTG 
GGCTATGAGC AGGCCAACTG CAAAGGGGAG CAGTTCGTGT TTGAGAAGGG TGAGTACCCC 
CGCTGGGACT CGTGGACCAG CAGTCGGAGG ACGGACTCGC TCAGCTCCCT GAGGCCCATC 
AAAGTGGACA GCCAGGAGCA TAAGATCACC CTGTATGAGA ACCCCAACTT CACGGGGAAG 
AAGATGGAGG TGATAGACGA TGACGTGCCC AGCTTCCACG CCCACGGCTA CCAGGAGAAG 
GTGTCTTCCG TGCGTGTGCA GAGCGGCACG TGGGTTGGCT ACCAGTACCC CGGCTACCGC 
GGGCTGCAGT ACCTGCTGGA GAAGGGCGAT TACAAGGACA GCGGTGACTT TGGGGCCCCC 
CAGCCCCAGG TGCAGTCCGT GCGCCGCATC CGGGACATGC AGTGGCACCA GCGGGGCGCC 
TTCCACCCCT CCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRYBB2 ( XM_024977034.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832802.1 Bos taurus crystallin beta B2 (CRYBB2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa224160
Clone ID Related Accession (Same CDS sequence)	XM_024977034.1
Accession Version	XM_024977034.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	beta-crystallin B2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAG ATCACCAGAC CCAAGCGGGC AAGCCGCAGC CCCTCAACCC CAAGATCATC 
ATCTTTGAGC AGGAGAACTT CCAGGGTCAC TCGCATGAGC TCAACGGGCC CTGCCCCAAC 
CTGAAGGAGA CTGGCGTGGA GAAGGCGGGC TCCGTCCTGG TGCAGGCTGG ACCCTGGGTG 
GGCTATGAGC AGGCCAACTG CAAAGGGGAG CAGTTCGTGT TTGAGAAGGG TGAGTACCCC 
CGCTGGGACT CGTGGACCAG CAGTCGGAGG ACGGACTCGC TCAGCTCCCT GAGGCCCATC 
AAAGTGGACA GCCAGGAGCA TAAGATCACC CTGTATGAGA ACCCCAACTT CACGGGGAAG 
AAGATGGAGG TGATAGACGA TGACGTGCCC AGCTTCCACG CCCACGGCTA CCAGGAGAAG 
GTGTCTTCCG TGCGTGTGCA GAGCGGCACG TGGGTTGGCT ACCAGTACCC CGGCTACCGC 
GGGCTGCAGT ACCTGCTGGA GAAGGGCGAT TACAAGGACA GCGGTGACTT TGGGGCCCCC 
CAGCCCCAGG TGCAGTCCGT GCGCCGCATC CGGGACATGC AGTGGCACCA GCGGGGCGCC 
TTCCACCCCT CCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832802.1
CDS	1068..1685
Translation	MASDHQTQAGKPQPLNPKIIIFEQENFQGHSHELNGPCPNLKETGVEKAGSVLVQAGPWVGYEQANCKGEQFVFEKGEYPRWDSWTSSRRTDSLSSLRPIKVDSQEHKITLYENPNFTGKKMEVIDDDVPSFHAHGYQEKVSSVRVQSGTWVGYQYPGYRGLQYLLEKGDYKDSGDFGAPQPQVQSVRRIRDMQWHQRGAFHPSS

Target ORF information:

RefSeq Version	XM_024977034.1
Organism	Bos taurus(cattle)
Definition	Bos taurus crystallin beta B2 (CRYBB2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024977034.1

ORF Insert Sequence:

ATGGCCTCAG ATCACCAGAC CCAAGCGGGC AAGCCGCAGC CCCTCAACCC CAAGATCATC 
ATCTTTGAGC AGGAGAACTT CCAGGGTCAC TCGCATGAGC TCAACGGGCC CTGCCCCAAC 
CTGAAGGAGA CTGGCGTGGA GAAGGCGGGC TCCGTCCTGG TGCAGGCTGG ACCCTGGGTG 
GGCTATGAGC AGGCCAACTG CAAAGGGGAG CAGTTCGTGT TTGAGAAGGG TGAGTACCCC 
CGCTGGGACT CGTGGACCAG CAGTCGGAGG ACGGACTCGC TCAGCTCCCT GAGGCCCATC 
AAAGTGGACA GCCAGGAGCA TAAGATCACC CTGTATGAGA ACCCCAACTT CACGGGGAAG 
AAGATGGAGG TGATAGACGA TGACGTGCCC AGCTTCCACG CCCACGGCTA CCAGGAGAAG 
GTGTCTTCCG TGCGTGTGCA GAGCGGCACG TGGGTTGGCT ACCAGTACCC CGGCTACCGC 
GGGCTGCAGT ACCTGCTGGA GAAGGGCGAT TACAAGGACA GCGGTGACTT TGGGGCCCCC 
CAGCCCCAGG TGCAGTCCGT GCGCCGCATC CGGGACATGC AGTGGCACCA GCGGGGCGCC 
TTCCACCCCT CCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Detection and structural features of the betaB2-B3-crystallin heterodimer by radical probe mass spectrometry (RP-MS).
Journal of mass spectrometry : JMS44(5)803-12(2009 May)
Diemer H,Atmanene C,Sanglier S,Morrissey B,Van Dorsselaer A,Downard KM

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Identification of the primary targets of carbamylation in bovine lens proteins by mass spectrometry.
Current eye research33(11)963-76(2008 Nov)
Zhang J,Yan H,Harding JJ,Liu ZX,Wang X,Ruan YS

Association of partially folded lens betaB2-crystallins with the alpha-crystallin molecular chaperone.
The Biochemical journal409(3)691-9(2008 Feb)
Evans P,Slingsby C,Wallace BA

Calcium-binding to lens betaB2- and betaA3-crystallins suggests that all beta-crystallins are calcium-binding proteins.
The FEBS journal274(16)4135-47(2007 Aug)
Jobby MK,Sharma Y