NEFL cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	NEFL
Entrez Gene ID	281348
Full Name	neurofilament light
General protein information	Preferred Names neurofilament light Names neurofilament light polypeptide 68 kDa neurofilament protein NF-L micro glutamic acid-rich protein neurofilament triplet L protein neurofilament, light polypeptide 68kDa
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174121.1	NP_776546.1	neurofilament light polypeptide

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NEFL	XP_519665.2
Xenopus tropicalis (tropical clawed frog)	nefl	XP_002932628.2
Canis lupus familiaris (dog)	NEFL	XP_003639928.1
Bos taurus (cattle)	NEFL	NP_776546.1
Rattus norvegicus (Norway rat)	Nefl	NP_113971.1
Danio rerio (zebrafish)	nefla	XP_001333768.2
Homo sapiens (human)	NEFL	NP_006149.2
Danio rerio (zebrafish)	neflb	NP_001034927.1
Gallus gallus (chicken)	NEFL	XP_001232615.1
	NEFL	XP_001107100.1
Mus musculus (house mouse)	Nefl	NP_035040.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Identification of endogenous phosphorylation sites of bovine medium and low molecular weight neurofilament proteins by tandem mass spectrometry.
Trimpin S, Mixon AE, Stapels MD, Kim MY, Spencer PS, Deinzer ML
Biochemistry43(7)2091-105(2004 Feb)

Brain micro glutamic acid-rich protein is the C-terminal endpiece of the neurofilament 68-kDa protein as determined by the primary sequence.
Isobe T, Okuyama T
FEBS letters182(2)389-92(1985 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NEFL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEFL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa41695	NM_174121.1 Latest version!	Bos taurus neurofilament light (NEFL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NEFL ( NM_174121.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776546.1 Bos taurus neurofilament light (NEFL), mRNA.

Price & Availability↑

CloneID	OBa41695
Clone ID Related Accession (Same CDS sequence)	NM_174121.1
Accession Version	NM_174121.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Bos taurus(cattle)
Product	neurofilament light polypeptide
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U83919.1. Sequence Note: This sequence has been modified as follows: removed 17 bp suspected to be vector contamination from the 5' end. ##Evidence-Data-START## Transcript exon combination :: U83919.1, EH208158.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145443, SAMN06621977 [ECO:0000348] ##Evidence-Data-END##

ATGAGTTCCT TCAGCTACGA GCCGTACTAC TCGACCTCCT ACAAACGGCG CTACGTGGAG 
ACGCCCCGGG TGCACATCTC CAGCGTGCGC AGCGGCTACA GCACCGCGCG CTCCGCTTAC 
TCCAGCTACT CCGCGCCCGT CTCCTCCTCG CTGTCCGTGC GGCGCAGCTA CTCCTCCAGC 
TCCGGTTCCT TGATGCCCAG CCTCGAGAGC CTCGACCTGA GCCAGGTCGC CGCCATCAGC 
AACGACCTCA AGTCGATCCG CACCCAGGAG AAGGCGCAGC TGCAGGACCT CAACGACCGT 
TTCGCCAGCT TCATCGAGCG CGTGCACGAG CTGGAGCAGC AGAACAAGGT CCTGGAAGCC 
GAGCTGCTGG TGCTGCGCCA GAAGCACTCC GAGCCCTCCC GCTTCCGGGC GCTCTACGAG 
CAGGAGATCC GCGATCTGCG TCTGGCAGCG GAAGACGCCA CCAACGAGAA GCAGGCACTC 
CAGGGCGAGC GCGAGGGGCT GGAGGAGACC CTGCGCAACC TGCAGGCGCG CTACGAAGAG 
GAGGTGCTGA GCCGTGAGGA CGCCGAGGGC CGGCTGATGG AAGCGCGCAA AGGGGCCGAC 
GAGGCGGCTC TCGCCCGCGC CGAGCTCGAA AAGCGCATCG ACAGCCTGAT GGACGAAATC 
GCCTTTCTGA AGAAAGTGCA CGAAGAGGAG ATCGCCGAGC TGCAGGCGCA GATCCAGTAC 
GCACAGATCT CCGTGGAGAT GGACGTGTCC TCCAAGCCCG ACCTCTCCGC CGCGCTCAAG 
GACATCCGCG CCCAGTATGA GAAGCTGGCC GCCAAGAACA TGCAGAATGC CGAAGAATGG 
TTCAAGAGCC GCTTCACGGT GCTGACCGAG AGCGCCGCCA AGAACACCGA TGCTGTGCGC 
GCCGCCAAGG ACGAGGTGTC CGAGAGCCGC CGCCTGCTTA AGGCCAAGAC CCTGGAGATC 
GAAGCATGCC GGGGCATGAA CGAAGCCCTG GAGAAGCAAT TGCAGGAACT AGAGGACAAG 
CAGAACGCCG ACATTAGCGC TATGCAGGAC ACAATCAACA AATTGGAAAA TGAATTGAGA 
ACCACAAAGA GCGAAATGGC AAGATACCTC AAGGAATACC AAGACCTCCT CAATGTYAAG 
ATGGCTTTGG ACATTGAGAT TGCAGCTTAC AGGAAACTCT TGGAAGGTGA GGAGACCCGG 
CTCAGTTTCA CCAGTGTGGG CAGCCTAACC ACCGGCTACA CCCAGAGCTC CCAGGTCTTT 
GGCCGATCTG CCTATGGAGG TTTGCAGACC AGCTCCTATT TGATGTCTGC CCGCTCCTTC 
CCGTCCTACT ACACCAGCCA CGTCCAGGAG GAGCAGATAG AGGTGGAGGA GACCATCGAG 
GCTGCTAAAG CTGAGGAAGC CAAGGACGAG CCGCCCTCTG AAGGAGAAGC AGAGGAAGAG 
GAGAAGGAGA AGGAAGAGGC TGAGGCTGAG GCTGAGGCTG AGGCCGAAGC TGAGGCTGAG 
GAAGAGGAGG GAGCCCAAGA GGAAGAAGCT GCCAAGGAAG ATGCTGAGGA AGCAAAGGAG 
GAAGAAGGAG GTGAAGGCGA AGAAGCAGAA GAAACCAAAG AAGCAGAGGA GGAAGAGAAA 
AAAGATGAAG GTGCTGGGGA GGAGCAAGCC ACTAAGAAGA AAGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776546.1
CDS	86..1753
Translation	MSSFSYEPYYSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLESLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEIAFLKKVHEEEIAELQAQIQYAQISVEMDVSSKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRTTKSEMARYLKEYQDLLNnullKMALDIEIAAYRKLLEGEETRLSFTSVGSLTTGYTQSSQVFGRSAYGGLQTSSYLMSARSFPSYYTSHVQEEQIEVEETIEAAKAEEAKDEPPSEGEAEEEEKEKEEAEAEAEAEAEAEAEEEEGAQEEEAAKEDAEEAKEEEGGEGEEAEETKEAEEEEKKDEGAGEEQATKKKD

Target ORF information:

RefSeq Version	NM_174121.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofilament light (NEFL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174121.1

ORF Insert Sequence:

ATGAGTTCCT TCAGCTACGA GCCGTACTAC TCGACCTCCT ACAAACGGCG CTACGTGGAG 
ACGCCCCGGG TGCACATCTC CAGCGTGCGC AGCGGCTACA GCACCGCGCG CTCCGCTTAC 
TCCAGCTACT CCGCGCCCGT CTCCTCCTCG CTGTCCGTGC GGCGCAGCTA CTCCTCCAGC 
TCCGGTTCCT TGATGCCCAG CCTCGAGAGC CTCGACCTGA GCCAGGTCGC CGCCATCAGC 
AACGACCTCA AGTCGATCCG CACCCAGGAG AAGGCGCAGC TGCAGGACCT CAACGACCGT 
TTCGCCAGCT TCATCGAGCG CGTGCACGAG CTGGAGCAGC AGAACAAGGT CCTGGAAGCC 
GAGCTGCTGG TGCTGCGCCA GAAGCACTCC GAGCCCTCCC GCTTCCGGGC GCTCTACGAG 
CAGGAGATCC GCGATCTGCG TCTGGCAGCG GAAGACGCCA CCAACGAGAA GCAGGCACTC 
CAGGGCGAGC GCGAGGGGCT GGAGGAGACC CTGCGCAACC TGCAGGCGCG CTACGAAGAG 
GAGGTGCTGA GCCGTGAGGA CGCCGAGGGC CGGCTGATGG AAGCGCGCAA AGGGGCCGAC 
GAGGCGGCTC TCGCCCGCGC CGAGCTCGAA AAGCGCATCG ACAGCCTGAT GGACGAAATC 
GCCTTTCTGA AGAAAGTGCA CGAAGAGGAG ATCGCCGAGC TGCAGGCGCA GATCCAGTAC 
GCACAGATCT CCGTGGAGAT GGACGTGTCC TCCAAGCCCG ACCTCTCCGC CGCGCTCAAG 
GACATCCGCG CCCAGTATGA GAAGCTGGCC GCCAAGAACA TGCAGAATGC CGAAGAATGG 
TTCAAGAGCC GCTTCACGGT GCTGACCGAG AGCGCCGCCA AGAACACCGA TGCTGTGCGC 
GCCGCCAAGG ACGAGGTGTC CGAGAGCCGC CGCCTGCTTA AGGCCAAGAC CCTGGAGATC 
GAAGCATGCC GGGGCATGAA CGAAGCCCTG GAGAAGCAAT TGCAGGAACT AGAGGACAAG 
CAGAACGCCG ACATTAGCGC TATGCAGGAC ACAATCAACA AATTGGAAAA TGAATTGAGA 
ACCACAAAGA GCGAAATGGC AAGATACCTC AAGGAATACC AAGACCTCCT CAATGTYAAG 
ATGGCTTTGG ACATTGAGAT TGCAGCTTAC AGGAAACTCT TGGAAGGTGA GGAGACCCGG 
CTCAGTTTCA CCAGTGTGGG CAGCCTAACC ACCGGCTACA CCCAGAGCTC CCAGGTCTTT 
GGCCGATCTG CCTATGGAGG TTTGCAGACC AGCTCCTATT TGATGTCTGC CCGCTCCTTC 
CCGTCCTACT ACACCAGCCA CGTCCAGGAG GAGCAGATAG AGGTGGAGGA GACCATCGAG 
GCTGCTAAAG CTGAGGAAGC CAAGGACGAG CCGCCCTCTG AAGGAGAAGC AGAGGAAGAG 
GAGAAGGAGA AGGAAGAGGC TGAGGCTGAG GCTGAGGCTG AGGCCGAAGC TGAGGCTGAG 
GAAGAGGAGG GAGCCCAAGA GGAAGAAGCT GCCAAGGAAG ATGCTGAGGA AGCAAAGGAG 
GAAGAAGGAG GTGAAGGCGA AGAAGCAGAA GAAACCAAAG AAGCAGAGGA GGAAGAGAAA 
AAAGATGAAG GTGCTGGGGA GGAGCAAGCC ACTAAGAAGA AAGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Identification of endogenous phosphorylation sites of bovine medium and low molecular weight neurofilament proteins by tandem mass spectrometry.
Biochemistry43(7)2091-105(2004 Feb)
Trimpin S,Mixon AE,Stapels MD,Kim MY,Spencer PS,Deinzer ML

Brain micro glutamic acid-rich protein is the C-terminal endpiece of the neurofilament 68-kDa protein as determined by the primary sequence.
FEBS letters182(2)389-92(1985 Mar)
Isobe T,Okuyama T