CHGB cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHGB
Entrez Gene ID	281071
Full Name	chromogranin B
General protein information	Preferred Names chromogranin B Names secretogranin-1 cgB chromogranin B (secretogranin 1) secretogranin I sgI
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010811358.2	XP_010809660.1	secretogranin-1 isoform X1
NM_181006.2	NP_851349.1	secretogranin-1 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CHGB	XP_534354.3
Gallus gallus (chicken)	CHGB	XP_419377.1
	CHGB	XP_001112965.2
Bos taurus (cattle)	CHGB	NP_851349.1
Rattus norvegicus (Norway rat)	Chgb	NP_036658.2
Mus musculus (house mouse)	Chgb	NP_031720.1
Homo sapiens (human)	CHGB	NP_001810.2
Pan troglodytes (chimpanzee)	CHGB	XP_525418.3

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Presence of secretogranin II and high-capacity, low-affinity Ca2+ storage role in nucleoplasmic Ca2+ store vesicles.
Yoo SH, Chu SY, Kim KD, Huh YH
Biochemistry46(50)14663-71(2007 Dec)

Subcellular distribution of chromogranins A and B in bovine adrenal chromaffin cells.
Huh YH, Bahk SJ, Ghee JY, Yoo SH
FEBS letters579(23)5145-51(2005 Sep)

Presence of a nucleoplasmic complex composed of the inositol 1,4,5-trisphosphate receptor/Ca2+ channel, chromogranin B, and phospholipids.
Yoo SH, Nam SW, Huh SK, Park SY, Huh YH
Biochemistry44(25)9246-54(2005 Jun)

A functional interaction between chromogranin B and the inositol 1,4,5-trisphosphate receptor/Ca2+ channel.
Thrower EC, Choe CU, So SH, Jeon SH, Ehrlich BE, Yoo SH
The Journal of biological chemistry278(50)49699-706(2003 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Chromogranin B was found to co-localize with secretogranin II and IP3 receptors in the nucleus, more specifically, in the IP3-sensitive nucleoplasmic Ca2+ store vesicles.
Title: Presence of secretogranin II and high-capacity, low-affinity Ca2+ storage role in nucleoplasmic Ca2+ store vesicles.

determination of the subcellular distribution of chromogranins A and B in chromaffin cells; results suggest that chromogranins are at the center of intracellular Ca(2+) homeostasis in secretory cells
Title: Subcellular distribution of chromogranins A and B in bovine adrenal chromaffin cells.

colocalization of all three inositol 1,4,5-trisphosphate receptor (IP(3)R)isoforms with chromogranin B to the extent that the majority of each IP(3)R isoform-labeling gold particles found in the nucleoplasm
Title: Presence of a nucleoplasmic complex composed of the inositol 1,4,5-trisphosphate receptor/Ca2+ channel, chromogranin B, and phospholipids.

CGB may play an important modulatory role in the control of Ca2+ release from the endoplasmic reticulum
Title: A functional interaction between chromogranin B and the inositol 1,4,5-trisphosphate receptor/Ca2+ channel.

chromogranin B has a role in the IP(3)-mediated Ca(2+) release mechanism of secretory granules
Title: Inositol 1,4,5-trisphosphate receptor/Ca(2+) channel modulatory role of chromogranins A and B.

The following CHGB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHGB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa41571	NM_181006.2 Latest version!	Bos taurus chromogranin B (CHGB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa45066	XM_010811358.2 Latest version!	Bos taurus chromogranin B (CHGB), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHGB ( NM_181006.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_851349.1 Bos taurus chromogranin B (CHGB), mRNA.

Price & Availability↑

CloneID	OBa41571
Clone ID Related Accession (Same CDS sequence)	NM_181006.2
Accession Version	NM_181006.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Bos taurus(cattle)
Product	secretogranin-1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U88551.1. On Jun 3, 2003 this sequence version replaced NM_181006.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U88551.1, X55489.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGCAGCCGG CCGCCCTTCT CGGCCTTCTG GGAGCCACGG TGGTTGCAGC CGTCAGCTCT 
ATGCCAGTGG ACATCAGGAA CCACAATGAA GAAGTGGTGA CTCACTGCAT CATCGAGGTC 
CTCTCAAATG CCCTATTGAA GTCCAGCGCT CCACCCATCA CCCCTGAGTG CCGACAAGTC 
CTTAAGAAGA ATGGAAAAGA GCTCAAAGAT GAAGAGAAAA GTGAAAATGA AAACACAAGG 
TTTGAAGTGA GATTGTTGAG AGACCCAGCT GACACCTCAG AAGCCCCCGG GCTCTCCAGT 
AGGGAGGACT CAGGGGAGGG GGATGCCCAA GTCCCAACAG TAGCAGACAC GGAGAGCGGT 
GGGCATAGCC GAGAGCGGGC AGGTGAGCCC CCGGGAAGTC AAGTGGCCAA AGAAGCAAAG 
ACACGCTATT CTAAGAGCGA GGGACAGAAC AGGGAGGAAG AAATGGTGAA ATACCAGAAA 
AGGGAACGTG GGGAAGTTGG CAGTGAGGAG AGACTGTCTG AAGGGCCGGG AAAGGCACAA 
ATGGCTTTTC TCAACCAAAG AAACCAGACT CCGGCTAAGA AAGAGGAGTT AGTGTCCAGA 
TATGATACAC AGTCTGCCAG GGGCCTTGAG AAGTCGCACA GCCGGGAAAG GAGCAGCCAG 
GAGAGTGGAG AGGAGACCAA GAGCCAGGAG AACTGGCCCC AAGAGCTGCA ACGCCATCCG 
GAGGGCCAGG AAGCACCCGG AGAAAGTGAA GAGGATGCCA GCCCCGAGGT GGACAAACGG 
CGCTCGAGGC CAAGACACCA CCACGGGAGG AGCAGGCCCG ACAGGTCCTC CCAGGAGGGG 
AATCCTCCCC TCGAGGAGGA GTCACACGTG GGCACGGGCA ACTCAGACGA AGAGAAAGCC 
CGCCATCCAG CCCACTTTAG GGCTTTGGAG GAGGGAGCCG AATATGGGGA GGAAGTGAGG 
AGACACTCAG CTGCCCAGGC TCCTGGAGAC TTGCAGGGGG CACGATTCGG GGGCAGAGGA 
CGTGGAGAGC ACCAGGCTCT AAGGCGTCCC AGCGAGGAGA GCCTAGAGCA GGAAAACAAG 
AGACATGGCC TCAGCCCGGA TCTAAACATG GCGCAGGGAT ACAGCGAGGA AAGCGAGGAA 
GAGAGGGGTC CGGCCCGGGG ACCCAGCTAC AGAGCCCGGG GAGGGGAGGC GGCGGCCTAC 
TCCACACTAG GCCAAACAGA TGAGAAACGG TTCTTGGGTG AAACGCACCA CCGTGTTCAG 
GAAAGCCAGA GGGACAAGGC GAGGCGCCGC CTACCAGGCG AGCTGAGAAA TTACCTCGAC 
TATGGTGAGG AAAAGGGTGA GGAAGCAGCC AGAGGGAAGT GGCAGCCGCA GGGAGACCCG 
CGAGACGCTG ACGAGAACAG GGAAGAGGCT AGGCTTCGAG GCAAACAGTA TGCTCCCCAT 
CACATCACTG AAAAGAGATT AGGGGAGCTA CTCAATCCAT TCTACGACCC TTCCCAGTGG 
AAGAGCAGCC GTTTTGAGAG AAAAGACCCC ATGGATGACA GTTTTCTTGA GGGTGAAGAG 
GAAAACGGGC TGACCTTGAA TGAGAAAAAT TTCTTCCCAG AATACAACTA TGACTGGTGG 
GAGAAAAAGC CCTTTGAAGA GGATGTAAAC TGGGGGTATG AGAAGAGAAA CCCGGTCCCC 
AAACTGGATC TAAAAAGGCA GTATGACCGA GTGGCCGAAC TGGACCAGCT CCTTCACTAC 
AGGAAGAAGT CAGCTGAGTT CCCAGACTTC TATGACTCCG AGGAGCAGGT GAGCCCACAA 
CACACAGCAG AAAATGAAGA GGAGAAGGCT GGCCAAGGAG TTCTGACGGA GGAAGAGGAA 
AAAGAACTTG AAAACTTGGC TGCGATGGAT TTGGAACTAC AGAAAATAGC TGAGAAGTTC 
AGTGGTACCC GAAGGGGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_851349.1
CDS	119..2059
Translation	MQPAALLGLLGATVVAAVSSMPVDIRNHNEEVVTHCIIEVLSNALLKSSAPPITPECRQVLKKNGKELKDEEKSENENTRFEVRLLRDPADTSEAPGLSSREDSGEGDAQVPTVADTESGGHSRERAGEPPGSQVAKEAKTRYSKSEGQNREEEMVKYQKRERGEVGSEERLSEGPGKAQMAFLNQRNQTPAKKEELVSRYDTQSARGLEKSHSRERSSQESGEETKSQENWPQELQRHPEGQEAPGESEEDASPEVDKRRSRPRHHHGRSRPDRSSQEGNPPLEEESHVGTGNSDEEKARHPAHFRALEEGAEYGEEVRRHSAAQAPGDLQGARFGGRGRGEHQALRRPSEESLEQENKRHGLSPDLNMAQGYSEESEEERGPARGPSYRARGGEAAAYSTLGQTDEKRFLGETHHRVQESQRDKARRRLPGELRNYLDYGEEKGEEAARGKWQPQGDPRDADENREEARLRGKQYAPHHITEKRLGELLNPFYDPSQWKSSRFERKDPMDDSFLEGEEENGLTLNEKNFFPEYNYDWWEKKPFEEDVNWGYEKRNPVPKLDLKRQYDRVAELDQLLHYRKKSAEFPDFYDSEEQVSPQHTAENEEEKAGQGVLTEEEEKELENLAAMDLELQKIAEKFSGTRRG

Target ORF information:

RefSeq Version	NM_181006.2
Organism	Bos taurus(cattle)
Definition	Bos taurus chromogranin B (CHGB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181006.2

ORF Insert Sequence:

ATGCAGCCGG CCGCCCTTCT CGGCCTTCTG GGAGCCACGG TGGTTGCAGC CGTCAGCTCT 
ATGCCAGTGG ACATCAGGAA CCACAATGAA GAAGTGGTGA CTCACTGCAT CATCGAGGTC 
CTCTCAAATG CCCTATTGAA GTCCAGCGCT CCACCCATCA CCCCTGAGTG CCGACAAGTC 
CTTAAGAAGA ATGGAAAAGA GCTCAAAGAT GAAGAGAAAA GTGAAAATGA AAACACAAGG 
TTTGAAGTGA GATTGTTGAG AGACCCAGCT GACACCTCAG AAGCCCCCGG GCTCTCCAGT 
AGGGAGGACT CAGGGGAGGG GGATGCCCAA GTCCCAACAG TAGCAGACAC GGAGAGCGGT 
GGGCATAGCC GAGAGCGGGC AGGTGAGCCC CCGGGAAGTC AAGTGGCCAA AGAAGCAAAG 
ACACGCTATT CTAAGAGCGA GGGACAGAAC AGGGAGGAAG AAATGGTGAA ATACCAGAAA 
AGGGAACGTG GGGAAGTTGG CAGTGAGGAG AGACTGTCTG AAGGGCCGGG AAAGGCACAA 
ATGGCTTTTC TCAACCAAAG AAACCAGACT CCGGCTAAGA AAGAGGAGTT AGTGTCCAGA 
TATGATACAC AGTCTGCCAG GGGCCTTGAG AAGTCGCACA GCCGGGAAAG GAGCAGCCAG 
GAGAGTGGAG AGGAGACCAA GAGCCAGGAG AACTGGCCCC AAGAGCTGCA ACGCCATCCG 
GAGGGCCAGG AAGCACCCGG AGAAAGTGAA GAGGATGCCA GCCCCGAGGT GGACAAACGG 
CGCTCGAGGC CAAGACACCA CCACGGGAGG AGCAGGCCCG ACAGGTCCTC CCAGGAGGGG 
AATCCTCCCC TCGAGGAGGA GTCACACGTG GGCACGGGCA ACTCAGACGA AGAGAAAGCC 
CGCCATCCAG CCCACTTTAG GGCTTTGGAG GAGGGAGCCG AATATGGGGA GGAAGTGAGG 
AGACACTCAG CTGCCCAGGC TCCTGGAGAC TTGCAGGGGG CACGATTCGG GGGCAGAGGA 
CGTGGAGAGC ACCAGGCTCT AAGGCGTCCC AGCGAGGAGA GCCTAGAGCA GGAAAACAAG 
AGACATGGCC TCAGCCCGGA TCTAAACATG GCGCAGGGAT ACAGCGAGGA AAGCGAGGAA 
GAGAGGGGTC CGGCCCGGGG ACCCAGCTAC AGAGCCCGGG GAGGGGAGGC GGCGGCCTAC 
TCCACACTAG GCCAAACAGA TGAGAAACGG TTCTTGGGTG AAACGCACCA CCGTGTTCAG 
GAAAGCCAGA GGGACAAGGC GAGGCGCCGC CTACCAGGCG AGCTGAGAAA TTACCTCGAC 
TATGGTGAGG AAAAGGGTGA GGAAGCAGCC AGAGGGAAGT GGCAGCCGCA GGGAGACCCG 
CGAGACGCTG ACGAGAACAG GGAAGAGGCT AGGCTTCGAG GCAAACAGTA TGCTCCCCAT 
CACATCACTG AAAAGAGATT AGGGGAGCTA CTCAATCCAT TCTACGACCC TTCCCAGTGG 
AAGAGCAGCC GTTTTGAGAG AAAAGACCCC ATGGATGACA GTTTTCTTGA GGGTGAAGAG 
GAAAACGGGC TGACCTTGAA TGAGAAAAAT TTCTTCCCAG AATACAACTA TGACTGGTGG 
GAGAAAAAGC CCTTTGAAGA GGATGTAAAC TGGGGGTATG AGAAGAGAAA CCCGGTCCCC 
AAACTGGATC TAAAAAGGCA GTATGACCGA GTGGCCGAAC TGGACCAGCT CCTTCACTAC 
AGGAAGAAGT CAGCTGAGTT CCCAGACTTC TATGACTCCG AGGAGCAGGT GAGCCCACAA 
CACACAGCAG AAAATGAAGA GGAGAAGGCT GGCCAAGGAG TTCTGACGGA GGAAGAGGAA 
AAAGAACTTG AAAACTTGGC TGCGATGGAT TTGGAACTAC AGAAAATAGC TGAGAAGTTC 
AGTGGTACCC GAAGGGGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHGB ( XM_010811358.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010809660.1 Bos taurus chromogranin B (CHGB), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa45066
Clone ID Related Accession (Same CDS sequence)	XM_010811358.2
Accession Version	XM_010811358.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	secretogranin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104370.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010811358.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCGG CCGCCCTTCT CGGCCTTCTG GGAGCCACGG TGGTTGCAGC CGTCAGCTCT 
ATGCCAGTGG ACATCAGGAA CCACAATGAA GAAGTGGTGA CTCACTGCAT CATCGAGGTC 
CTCTCAAATG CCCTATTGAA GTCCAGCGCT CCACCCATCA CCCCTGAGTG CCGACAAGTC 
CTGAAGAAGA ATGGAAAAGA GCTCAAAGAT GAAGAGAAAA GTGAAAATGA AAACACAAGG 
TTTGAAGTGA GATTGTTGAG AGACCCAGCT GACACCTCAG AAGCCCCCGG GCTCTCCAGT 
AGGGAGGACT CAGGGGAGGG GGATGCCCAA GTCCCAACAG TAGCAGACAC GGAGAGCGGT 
GGGCATAGCC GAGAGCGGGC AGGTGAGCCC CCGGGAAGTC AAGTGGCCAA AGAAGCAAAG 
ACACGCTATT CTAAGAGCGA GGGACAGAAC AGGGAGGAAG AAATGGTGAA ATACCAGAAA 
AGGGAACGTG GGGAAGTTGG CAGTGAGGAG AGACTGTCTG AAGGGCCGGG AAAGGCACAA 
ATGGCTTTTC TCAACCAAAG AAACCAGACT CCGGCTAAGA AAGAGGAGTT AGTGTCCAGA 
TATGATACAC AGTCTGCCAG GGGCCTTGAG AAGTCGCACA GCCGGGAAAG GAGCAGCCAG 
GAGAGTGGAG AGGAGACCAA GAGCCAGGAG AACTGGCCCC AAGAGCTGCA ACGCCATCCG 
GAGGGCCAGG AAGCACCCGG AGAAAGTGAA GAGGATGCCA GCCCCGAGGT GGACAAACGG 
CGCTCGAGGC CAAGACACCA CCACGGGAGG AGCAGGCCCG ACAGGTCCTC CCAGGAGGGG 
AATCCTCCCC TCGAGGAGGA GTCACACGTG GGCACGGGCA ACTCAGACGA AGAGAAAGCC 
CGCCATCCAG CCCACTTTAG GGCTTTGGAG GAGGGAGCCG AATATGGGGA GGAAGTGAGG 
AGACACTCAG CTGCCCAGGC TCCTGGAGAC TTGCAGGGGG CACGATTCGG GGGCAGAGGA 
CGTGGAGAGC ACCAGGCTCT AAGGCGTCCC AGCGAGGAGA GCCTAGAGCA GGAAAACAAG 
AGACATGGCC TCAGCCCGGA TCTAAACATG GCGCAGGGAT ACAGCGAGGA AAGCGAGGAA 
GAGAGGGGTC CGGCCCGGGG ACCCAGCTAC AGAGCCCGGG GAGGGGAGGC GGCGGCCTAC 
TCCACACTAG GCCAAACAGA TGAGAAACGG TTCTTGGGTG AAACGCACCA CCGTGTTCAG 
GAAAGCCAGA GGGACAAGGC GAGGCGCCGC CTACCAGGCG AGCTGAGAAA TTACCTCGAC 
TATGGTGAGG AAAAGGGTGA GGAAGCAGCC AGAGGGAAGT GGCAGCCGCA GGGAGACCCG 
CGAGACGCTG ACGAGAACAG GGAAGAGGCT AGGCTTCGAG GCAAACAGTA TGCTCCCCAT 
CACATCACTG AAAAGAGATT AGGGGAGCTA CTCAATCCAT TCTACGACCC TTCCCAGTGG 
AAGAGCAGCC GTTTTGAGAG AAAAGACCCC ATGGATGACA GTTTTCTTGA GGGTGAAGAG 
GAAAACGGGC TGACCTTGAA TGAGAAAAAT TTCTTCCCAG AATACAACTA TGACTGGTGG 
GAGAAAAAGC CCTTTGAAGA GGATGTAAAC TGGGGGTATG AGAAGAGAAA CCCGGTCCCC 
AAACTGGATC TAAAAAGGCA GTATGACCGA GTGGCCGAAC TGGACCAGCT CCTTCACTAC 
AGGAAGAAGT CAGCTGAGTT CCCAGACTTC TATGACTCCG AGGAGCAGGA AAAAGAACTT 
GAAAACTTGG CTGCGATGGA TTTGGAACTA CAGAAAATAG CTGAGAAGTT CAGTGGTACC 
CGAAGGGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010809660.1
CDS	220..2091
Translation	MQPAALLGLLGATVVAAVSSMPVDIRNHNEEVVTHCIIEVLSNALLKSSAPPITPECRQVLKKNGKELKDEEKSENENTRFEVRLLRDPADTSEAPGLSSREDSGEGDAQVPTVADTESGGHSRERAGEPPGSQVAKEAKTRYSKSEGQNREEEMVKYQKRERGEVGSEERLSEGPGKAQMAFLNQRNQTPAKKEELVSRYDTQSARGLEKSHSRERSSQESGEETKSQENWPQELQRHPEGQEAPGESEEDASPEVDKRRSRPRHHHGRSRPDRSSQEGNPPLEEESHVGTGNSDEEKARHPAHFRALEEGAEYGEEVRRHSAAQAPGDLQGARFGGRGRGEHQALRRPSEESLEQENKRHGLSPDLNMAQGYSEESEEERGPARGPSYRARGGEAAAYSTLGQTDEKRFLGETHHRVQESQRDKARRRLPGELRNYLDYGEEKGEEAARGKWQPQGDPRDADENREEARLRGKQYAPHHITEKRLGELLNPFYDPSQWKSSRFERKDPMDDSFLEGEEENGLTLNEKNFFPEYNYDWWEKKPFEEDVNWGYEKRNPVPKLDLKRQYDRVAELDQLLHYRKKSAEFPDFYDSEEQEKELENLAAMDLELQKIAEKFSGTRRG

Target ORF information:

RefSeq Version	XM_010811358.2
Organism	Bos taurus(cattle)
Definition	Bos taurus chromogranin B (CHGB), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010811358.2

ORF Insert Sequence:

ATGCAGCCGG CCGCCCTTCT CGGCCTTCTG GGAGCCACGG TGGTTGCAGC CGTCAGCTCT 
ATGCCAGTGG ACATCAGGAA CCACAATGAA GAAGTGGTGA CTCACTGCAT CATCGAGGTC 
CTCTCAAATG CCCTATTGAA GTCCAGCGCT CCACCCATCA CCCCTGAGTG CCGACAAGTC 
CTGAAGAAGA ATGGAAAAGA GCTCAAAGAT GAAGAGAAAA GTGAAAATGA AAACACAAGG 
TTTGAAGTGA GATTGTTGAG AGACCCAGCT GACACCTCAG AAGCCCCCGG GCTCTCCAGT 
AGGGAGGACT CAGGGGAGGG GGATGCCCAA GTCCCAACAG TAGCAGACAC GGAGAGCGGT 
GGGCATAGCC GAGAGCGGGC AGGTGAGCCC CCGGGAAGTC AAGTGGCCAA AGAAGCAAAG 
ACACGCTATT CTAAGAGCGA GGGACAGAAC AGGGAGGAAG AAATGGTGAA ATACCAGAAA 
AGGGAACGTG GGGAAGTTGG CAGTGAGGAG AGACTGTCTG AAGGGCCGGG AAAGGCACAA 
ATGGCTTTTC TCAACCAAAG AAACCAGACT CCGGCTAAGA AAGAGGAGTT AGTGTCCAGA 
TATGATACAC AGTCTGCCAG GGGCCTTGAG AAGTCGCACA GCCGGGAAAG GAGCAGCCAG 
GAGAGTGGAG AGGAGACCAA GAGCCAGGAG AACTGGCCCC AAGAGCTGCA ACGCCATCCG 
GAGGGCCAGG AAGCACCCGG AGAAAGTGAA GAGGATGCCA GCCCCGAGGT GGACAAACGG 
CGCTCGAGGC CAAGACACCA CCACGGGAGG AGCAGGCCCG ACAGGTCCTC CCAGGAGGGG 
AATCCTCCCC TCGAGGAGGA GTCACACGTG GGCACGGGCA ACTCAGACGA AGAGAAAGCC 
CGCCATCCAG CCCACTTTAG GGCTTTGGAG GAGGGAGCCG AATATGGGGA GGAAGTGAGG 
AGACACTCAG CTGCCCAGGC TCCTGGAGAC TTGCAGGGGG CACGATTCGG GGGCAGAGGA 
CGTGGAGAGC ACCAGGCTCT AAGGCGTCCC AGCGAGGAGA GCCTAGAGCA GGAAAACAAG 
AGACATGGCC TCAGCCCGGA TCTAAACATG GCGCAGGGAT ACAGCGAGGA AAGCGAGGAA 
GAGAGGGGTC CGGCCCGGGG ACCCAGCTAC AGAGCCCGGG GAGGGGAGGC GGCGGCCTAC 
TCCACACTAG GCCAAACAGA TGAGAAACGG TTCTTGGGTG AAACGCACCA CCGTGTTCAG 
GAAAGCCAGA GGGACAAGGC GAGGCGCCGC CTACCAGGCG AGCTGAGAAA TTACCTCGAC 
TATGGTGAGG AAAAGGGTGA GGAAGCAGCC AGAGGGAAGT GGCAGCCGCA GGGAGACCCG 
CGAGACGCTG ACGAGAACAG GGAAGAGGCT AGGCTTCGAG GCAAACAGTA TGCTCCCCAT 
CACATCACTG AAAAGAGATT AGGGGAGCTA CTCAATCCAT TCTACGACCC TTCCCAGTGG 
AAGAGCAGCC GTTTTGAGAG AAAAGACCCC ATGGATGACA GTTTTCTTGA GGGTGAAGAG 
GAAAACGGGC TGACCTTGAA TGAGAAAAAT TTCTTCCCAG AATACAACTA TGACTGGTGG 
GAGAAAAAGC CCTTTGAAGA GGATGTAAAC TGGGGGTATG AGAAGAGAAA CCCGGTCCCC 
AAACTGGATC TAAAAAGGCA GTATGACCGA GTGGCCGAAC TGGACCAGCT CCTTCACTAC 
AGGAAGAAGT CAGCTGAGTT CCCAGACTTC TATGACTCCG AGGAGCAGGA AAAAGAACTT 
GAAAACTTGG CTGCGATGGA TTTGGAACTA CAGAAAATAG CTGAGAAGTT CAGTGGTACC 
CGAAGGGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Presence of secretogranin II and high-capacity, low-affinity Ca2+ storage role in nucleoplasmic Ca2+ store vesicles.
Biochemistry46(50)14663-71(2007 Dec)
Yoo SH,Chu SY,Kim KD,Huh YH

Subcellular distribution of chromogranins A and B in bovine adrenal chromaffin cells.
FEBS letters579(23)5145-51(2005 Sep)
Huh YH,Bahk SJ,Ghee JY,Yoo SH

Presence of a nucleoplasmic complex composed of the inositol 1,4,5-trisphosphate receptor/Ca2+ channel, chromogranin B, and phospholipids.
Biochemistry44(25)9246-54(2005 Jun)
Yoo SH,Nam SW,Huh SK,Park SY,Huh YH

A functional interaction between chromogranin B and the inositol 1,4,5-trisphosphate receptor/Ca2+ channel.
The Journal of biological chemistry278(50)49699-706(2003 Dec)
Thrower EC,Choe CU,So SH,Jeon SH,Ehrlich BE,Yoo SH