Chgb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chgb
Entrez Gene ID	24259
Full Name	chromogranin B
General protein information	Preferred Names chromogranin B Names secretogranin-1 Chromogranin B, parathyroid secretory protein cgB chromogranin B (secretogranin 1) glucagonoma peptide secretogranin-I sgI
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q36
Summary	secretory granule protein expressed in neuroendocrine cells [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006235072.3	XP_006235134.1	secretogranin-1 isoform X1
NM_012526.2	NP_036658.2	secretogranin-1 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CHGB	XP_534354.3
Gallus gallus (chicken)	CHGB	XP_419377.1
	CHGB	XP_001112965.2
Bos taurus (cattle)	CHGB	NP_851349.1
Rattus norvegicus (Norway rat)	Chgb	NP_036658.2
Mus musculus (house mouse)	Chgb	NP_031720.1
Homo sapiens (human)	CHGB	NP_001810.2
Pan troglodytes (chimpanzee)	CHGB	XP_525418.3

Gene Ontology
Bibliography

Related articles in PubMed

A systems approach identifies co-signaling molecules of early growth response 1 transcription factor in immobilization stress.
Papanikolaou NA, Tillinger A, Liu X, Papavassiliou AG, Sabban EL
BMC systems biology8100(2014 Sep)

Chromogranin B: intra- and extra-cellular mechanisms to regulate catecholamine storage and release, in catecholaminergic cells and organisms.
Zhang K, Biswas N, Gayen JR, Miramontes-Gonzalez JP, Hightower CM, Mustapic M, Mahata M, Huang CT, Hook VY, Mahata SK, Vaingankar S, O'Connor DT
Journal of neurochemistry129(1)48-59(2014 Apr)

Beta cell chromogranin B is partially segregated in distinct granules and can be released separately from insulin in response to stimulation.
Giordano T, Brigatti C, Podini P, Bonifacio E, Meldolesi J, Malosio ML
Diabetologia51(6)997-1007(2008 Jun)

Chromogranin B regulates calcium signaling, nuclear factor kappaB activity, and brain natriuretic peptide production in cardiomyocytes.
Heidrich FM, Zhang K, Estrada M, Huang Y, Giordano FJ, Ehrlich BE
Circulation research102(10)1230-8(2008 May)

A sulfated, phosphorylated 7 kDa secreted peptide characterized by direct analysis of cell culture media.
Taylor SW, Sun C, Hsieh A, Andon NL, Ghosh SS
Journal of proteome research7(2)795-802(2008 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Possible involvement of Stat3 and Prlh1/Chgb up-regulation in the transition from short to repeated stress activation.
Title: A systems approach identifies co-signaling molecules of early growth response 1 transcription factor in immobilization stress.

When released extra-cellulary, CHGB proteolytic fragments exert a feedback effect to inhibit catecholamine secretion.
Title: Chromogranin B: intra- and extra-cellular mechanisms to regulate catecholamine storage and release, in catecholaminergic cells and organisms.

Stimulation with a secretagogue mixture induced parallel release of insulin and chromogranin B from pancreatic beta cells.
Title: Beta cell chromogranin B is partially segregated in distinct granules and can be released separately from insulin in response to stimulation.

CGB is a novel regulator of cardiomyocyte InsP(3)/Ca(2+)-dependent signaling, nuclear factor kappaB activity, and brain natriuretic peptide production
Title: Chromogranin B regulates calcium signaling, nuclear factor kappaB activity, and brain natriuretic peptide production in cardiomyocytes.

Chrombacin is a 60 residue anti-microbial neuroendocrine peptide that includes an unusual sulfotyrosine-, phosphoserine-containing motif.
Title: A sulfated, phosphorylated 7 kDa secreted peptide characterized by direct analysis of cell culture media.

The following Chgb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chgb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13618	NM_012526.2 Latest version!	Rattus norvegicus chromogranin B (Chgb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa26418	XM_006235072.3 Latest version!	Rattus norvegicus chromogranin B (Chgb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chgb ( NM_012526.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036658.2 Rattus norvegicus chromogranin B (Chgb), mRNA.

Price & Availability↑

CloneID	ORa13618
Clone ID Related Accession (Same CDS sequence)	NM_012526.2
Accession Version	NM_012526.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	secretogranin-1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB705583.1 and BC128743.1. On Jul 24, 2012 this sequence version replaced NM_012526.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC128743.1, AF019974.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END##

ATGCAGCGGG CCATGCTCCT CGGCCTCCTG GGCGCAGCAG CTCTGGCTGC TGTCATCTCA 
GCTCCAGTGG ATAACAGGGA TCACAATGAA GAAATGGTGA CTCGCTGCAT TATTGAAGTC 
CTCTCAAATG CCCTATCCAA GTCCAGTGCT CCAACCATCA CCCCTGAGTG CCGGCAAGTC 
CTGAGGAAGA GTGGAAAAGA GGTCAAAGGT GAAGAGAAAG GTGAAAATGA GAACTCGAAG 
TTTGAAGTAC GGTTGCTAAG AGACCCATCT GATGCCTCAG TAGGCCGCTG GGCCTCCAGT 
AGGGAGGAAA CAGGGGCTCC AGTAGAAGAC TCTCCAGGTC AGGCGAAGGT AGACAATGAG 
GAGTGGACAG GAGGAGGAGG ACACAGTCGA GAGGCAGTAG ATGACCAGGA GAGCCTCCAT 
CCCTCCAACC AACAAGTGTC CAAAGAAGCA AAGATACGCC ATTCGGAGGA GAGGGGCGGA 
AAAGAGGAGG AGGAGGAAGA AGGCAAGATT TATCCAAAGG GAGAGCATAG GGGAGATGCT 
GGTGAGGAGA AGAAACACAC TGAAGAATCT GGAGAGAAAC ACAACGCCTT CTCCAACAAA 
AGAAGCGAAG CTTCAGCTAA GAAAAAAGAG GAGTCAGTGG CCAGAGCAGA GGCACACTTC 
GTGGAGCTGG AGAAGACACA CAGCAGGGAG CAGAGCAGCC AGGAGAGTGG AGAGGAGACG 
AGGAGGCAGG AGAAACCACA GGAGCTTCCC GACCAGGACC AGAGCGAGGA AGAATCCGAG 
GAAGGCGAGG AAGGCGAGGA AGGCGCAACC TCAGAAGTAA CCAAACGCAG GCCCAGGCAC 
CACCACTGGA GAAGCCAGTC CAACAAGCCC TCTTACGAAG GGCGTCGTCC TCTGTCTGAG 
GAAAGGAAAC ATGCTGCTGG GGAGTCCAAG GATGCAAATG TGGCCACAGC CAATTTAGGG 
GAAAAAAGGG GCCACCACCT AGCTCACTAC CGGGCTTCAG AGGAAGAACC TGACTATGGG 
GAAGAATTGA GAAGCTATCC GGGCTTCCAG GCTCCCCAGG GACTGCAGTA CCAGGGCAGA 
GGAAGTGAAG AGGTCAGAGC CCCAAGCCCT CGGAGTGAGG AGAGCCAGGA AAAGGAGTAC 
AAGAGAAACC ACCCCGACTC TGAACTGGAA AGCACAGCAA ACAGACACAG TGAAGAAACT 
GAGGAAGAGA GGAGCTACGA GGGGGCAAAG GGACGACAAC ACAGAGGCAG GGGGCGGGAG 
CCAGGAGCTT ATCCTGCTCT TGACAGCAGA CAAGAAAAAA GGCTCCTGGA TGAAGGGCAT 
GACCCTGTTC ATGAAAGCCC AGTAGATACG GCAAAAAGGT ATCCACAAAG CAAATGGCAG 
GAACAGGAGA AAAACTACCT CAACTATGAC GAGGAAGGGG ACCAAGGAAG ATGGTGGCAG 
CAGGAAGAGC AGCTAGAACC GGAAGAAAGC AGGGAGGAAG TGAGCTTTCC AGACAGACAA 
TACGCGCCCT ACCCCACCAC TGAAAAGAGG AAGAGGCTAG GGGCGCTGTT CAACCCGTAC 
TTTGACCCTC TCCAGTGGAA GAATAGCGAC TTTGAGAAAA AAGGCAACCC AGATGACAGT 
TTCCTTGATG ATGACGGCGA AGACGGAAAT GGGGTGACAA TGACTGAGAA GAATTTCTTC 
CCAGAGTACA ACTATGACTG GTGGGAGAAA AGGCCCTTCT CGGAGGATGT GAATTGGGGA 
TATGAGAAGA GAAGCTTTGC CAGGGCCCCT CACTTGGACT TGAAAAGGCA ATACGACGAT 
GGAGTGGCTG AACTGGACCA GCTCCTTCAC TACAGGAAGA AGGCAGCCGA ATTTCCCGAC 
TTCTACGATT CAGAGGAGCA GATGGGGCCT CACCAGGAGG CAGAAGATGA AAAGGACAGG 
GCTGACCAGA GAGTTCTGAC CGAGGAAGAG AAAAAGGAAC TGGAGAACTT GGCGGCCATG 
GATCTGGAAC TGCAGAAAAT AGCGGAAAAG TTCAGCCAGC GGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036658.2
CDS	264..2291
Translation	MQRAMLLGLLGAAALAAVISAPVDNRDHNEEMVTRCIIEVLSNALSKSSAPTITPECRQVLRKSGKEVKGEEKGENENSKFEVRLLRDPSDASVGRWASSREETGAPVEDSPGQAKVDNEEWTGGGGHSREAVDDQESLHPSNQQVSKEAKIRHSEERGGKEEEEEEGKIYPKGEHRGDAGEEKKHTEESGEKHNAFSNKRSEASAKKKEESVARAEAHFVELEKTHSREQSSQESGEETRRQEKPQELPDQDQSEEESEEGEEGEEGATSEVTKRRPRHHHWRSQSNKPSYEGRRPLSEERKHAAGESKDANVATANLGEKRGHHLAHYRASEEEPDYGEELRSYPGFQAPQGLQYQGRGSEEVRAPSPRSEESQEKEYKRNHPDSELESTANRHSEETEEERSYEGAKGRQHRGRGREPGAYPALDSRQEKRLLDEGHDPVHESPVDTAKRYPQSKWQEQEKNYLNYDEEGDQGRWWQQEEQLEPEESREEVSFPDRQYAPYPTTEKRKRLGALFNPYFDPLQWKNSDFEKKGNPDDSFLDDDGEDGNGVTMTEKNFFPEYNYDWWEKRPFSEDVNWGYEKRSFARAPHLDLKRQYDDGVAELDQLLHYRKKAAEFPDFYDSEEQMGPHQEAEDEKDRADQRVLTEEEKKELENLAAMDLELQKIAEKFSQRG

Target ORF information:

RefSeq Version	NM_012526.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus chromogranin B (Chgb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012526.2

ORF Insert Sequence:

ATGCAGCGGG CCATGCTCCT CGGCCTCCTG GGCGCAGCAG CTCTGGCTGC TGTCATCTCA 
GCTCCAGTGG ATAACAGGGA TCACAATGAA GAAATGGTGA CTCGCTGCAT TATTGAAGTC 
CTCTCAAATG CCCTATCCAA GTCCAGTGCT CCAACCATCA CCCCTGAGTG CCGGCAAGTC 
CTGAGGAAGA GTGGAAAAGA GGTCAAAGGT GAAGAGAAAG GTGAAAATGA GAACTCGAAG 
TTTGAAGTAC GGTTGCTAAG AGACCCATCT GATGCCTCAG TAGGCCGCTG GGCCTCCAGT 
AGGGAGGAAA CAGGGGCTCC AGTAGAAGAC TCTCCAGGTC AGGCGAAGGT AGACAATGAG 
GAGTGGACAG GAGGAGGAGG ACACAGTCGA GAGGCAGTAG ATGACCAGGA GAGCCTCCAT 
CCCTCCAACC AACAAGTGTC CAAAGAAGCA AAGATACGCC ATTCGGAGGA GAGGGGCGGA 
AAAGAGGAGG AGGAGGAAGA AGGCAAGATT TATCCAAAGG GAGAGCATAG GGGAGATGCT 
GGTGAGGAGA AGAAACACAC TGAAGAATCT GGAGAGAAAC ACAACGCCTT CTCCAACAAA 
AGAAGCGAAG CTTCAGCTAA GAAAAAAGAG GAGTCAGTGG CCAGAGCAGA GGCACACTTC 
GTGGAGCTGG AGAAGACACA CAGCAGGGAG CAGAGCAGCC AGGAGAGTGG AGAGGAGACG 
AGGAGGCAGG AGAAACCACA GGAGCTTCCC GACCAGGACC AGAGCGAGGA AGAATCCGAG 
GAAGGCGAGG AAGGCGAGGA AGGCGCAACC TCAGAAGTAA CCAAACGCAG GCCCAGGCAC 
CACCACTGGA GAAGCCAGTC CAACAAGCCC TCTTACGAAG GGCGTCGTCC TCTGTCTGAG 
GAAAGGAAAC ATGCTGCTGG GGAGTCCAAG GATGCAAATG TGGCCACAGC CAATTTAGGG 
GAAAAAAGGG GCCACCACCT AGCTCACTAC CGGGCTTCAG AGGAAGAACC TGACTATGGG 
GAAGAATTGA GAAGCTATCC GGGCTTCCAG GCTCCCCAGG GACTGCAGTA CCAGGGCAGA 
GGAAGTGAAG AGGTCAGAGC CCCAAGCCCT CGGAGTGAGG AGAGCCAGGA AAAGGAGTAC 
AAGAGAAACC ACCCCGACTC TGAACTGGAA AGCACAGCAA ACAGACACAG TGAAGAAACT 
GAGGAAGAGA GGAGCTACGA GGGGGCAAAG GGACGACAAC ACAGAGGCAG GGGGCGGGAG 
CCAGGAGCTT ATCCTGCTCT TGACAGCAGA CAAGAAAAAA GGCTCCTGGA TGAAGGGCAT 
GACCCTGTTC ATGAAAGCCC AGTAGATACG GCAAAAAGGT ATCCACAAAG CAAATGGCAG 
GAACAGGAGA AAAACTACCT CAACTATGAC GAGGAAGGGG ACCAAGGAAG ATGGTGGCAG 
CAGGAAGAGC AGCTAGAACC GGAAGAAAGC AGGGAGGAAG TGAGCTTTCC AGACAGACAA 
TACGCGCCCT ACCCCACCAC TGAAAAGAGG AAGAGGCTAG GGGCGCTGTT CAACCCGTAC 
TTTGACCCTC TCCAGTGGAA GAATAGCGAC TTTGAGAAAA AAGGCAACCC AGATGACAGT 
TTCCTTGATG ATGACGGCGA AGACGGAAAT GGGGTGACAA TGACTGAGAA GAATTTCTTC 
CCAGAGTACA ACTATGACTG GTGGGAGAAA AGGCCCTTCT CGGAGGATGT GAATTGGGGA 
TATGAGAAGA GAAGCTTTGC CAGGGCCCCT CACTTGGACT TGAAAAGGCA ATACGACGAT 
GGAGTGGCTG AACTGGACCA GCTCCTTCAC TACAGGAAGA AGGCAGCCGA ATTTCCCGAC 
TTCTACGATT CAGAGGAGCA GATGGGGCCT CACCAGGAGG CAGAAGATGA AAAGGACAGG 
GCTGACCAGA GAGTTCTGAC CGAGGAAGAG AAAAAGGAAC TGGAGAACTT GGCGGCCATG 
GATCTGGAAC TGCAGAAAAT AGCGGAAAAG TTCAGCCAGC GGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chgb ( XM_006235072.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006235134.1 Rattus norvegicus chromogranin B (Chgb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa26418
Clone ID Related Accession (Same CDS sequence)	XM_006235072.3
Accession Version	XM_006235072.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	secretogranin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006235072.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCTGG CGAGCGGCAC CATGCAGCGG GCCATGCTCC TCGGCCTCCT GGGCGCAGCA 
GCTCTGGCTG AGCTTTTTGA AAGGACATCC ACAACCATCT GTGCTGTCAT CTCAGCTCCA 
GTGGATAACA GGGATCACAA TGAAGAAATG GTGACTCGCT GCATTATTGA AGTCCTCTCA 
AATGCCCTAT CCAAGTCCAG TGCTCCAACC ATCACCCCTG AGTGCCGGCA AGTCCTGAGG 
AAGAGTGGAA AAGAGGTCAA AGGTGAAGAG AAAGGTGAAA ATGAGAACTC GAAGTTTGAA 
GTACGGTTGC TAAGAGACCC ATCTGATGCC TCAGTAGGCC GCTGGGCCTC CAGTAGGGAG 
GAAACAGGGG CTCCAGTAGA AGACTCTCCA GGTCAGGCGA AGGTAGACAA TGAGGAGTGG 
ACAGGAGGAG GAGGACACAG TCGAGAGGCA GTAGATGACC AGGAGAGCCT CCATCCCTCC 
AACCAACAAG TGTCCAAAGA AGCAAAGATA CGCCATTCGG AGGAGAGGGG CGGAAAAGAG 
GAGGAGGAGG AAGAAGGCAA GATTTATCCA AAGGGAGAGC ATAGGGGAGA TGCTGGTGAG 
GAGAAGAAAC ACACTGAAGA ATCTGGAGAG AAACACAACG CCTTCTCCAA CAAAAGAAGC 
GAAGCTTCAG CTAAGAAAAA AGAGGAGTCA GTGGCCAGAG CAGAGGCACA CTTCGTGGAG 
CTGGAGAAGA CACACAGCAG GGAGCAGAGC AGCCAGGAGA GTGGAGAGGA GACGAGGAGG 
CAGGAGAAAC CACAGGAGCT TCCCGACCAG GACCAGAGCG AGGAAGAATC CGAGGAAGGC 
GAGGAAGGCG AGGAAGGCGC AACCTCAGAA GTAACCAAAC GCAGGCCCAG GCACCACCAC 
TGGAGAAGCC AGTCCAACAA GCCCTCTTAC GAAGGGCGTC GTCCTCTGTC TGAGGAAAGG 
AAACATGCTG CTGGGGAGTC CAAGGATGCA AATGTGGCCA CAGCCAATTT AGGGGAAAAA 
AGGGGCCACC ACCTAGCTCA CTACCGGGCT TCAGAGGAAG AACCTGACTA TGGGGAAGAA 
TTGAGAAGCT ATCCGGGCTT CCAGGCTCCC CAGGGACTGC AGTACCAGGG CAGAGGAAGT 
GAAGAGGTCA GAGCCCCAAG CCCTCGGAGT GAGGAGAGCC AGGAAAAGGA GTACAAGAGA 
AACCACCCCG ACTCTGAACT GGAAAGCACA GCAAACAGAC ACAGTGAAGA AACTGAGGAA 
GAGAGGAGCT ACGAGGGGGC AAAGGGACGA CAACACAGAG GCAGGGGGCG GGAGCCAGGA 
GCTTATCCTG CTCTTGACAG CAGACAAGAA AAAAGGCTCC TGGATGAAGG GCATGACCCT 
GTTCATGAAA GCCCAGTAGA TACGGCAAAA AGGTATCCAC AAAGCAAATG GCAGGAACAG 
GAGAAAAACT ACCTCAACTA TGACGAGGAA GGGGACCAAG GAAGATGGTG GCAGCAGGAA 
GAGCAGCTAG AACCGGAAGA AAGCAGGGAG GAAGTGAGCT TTCCAGACAG ACAATACGCG 
CCCTACCCCA CCACTGAAAA GAGGAAGAGG CTAGGGGCGC TGTTCAACCC GTACTTTGAC 
CCTCTCCAGT GGAAGAATAG CGACTTTGAG AAAAAAGGCA ACCCAGATGA CAGTTTCCTT 
GATGATGACG GCGAAGACGG AAATGGGGTG ACAATGACTG AGAAGAATTT CTTCCCAGAG 
TACAACTATG ACTGGTGGGA GAAAAGGCCC TTCTCGGAGG ATGTGAATTG GGGATATGAG 
AAGAGAAGCT TTGCCAGGGC CCCTCACTTG GACTTGAAAA GGCAATACGA CGATGGAGTG 
GCTGAACTGG ACCAGCTCCT TCACTACAGG AAGAAGGCAG CCGAATTTCC CGACTTCTAC 
GATTCAGAGG AGCAGATGGG GCCTCACCAG GAGGCAGAAG ATGAAAAGGA CAGGGCTGAC 
CAGAGAGTTC TGACCGAGGA AGAGAAAAAG GAACTGGAGA ACTTGGCGGC CATGGATCTG 
GAACTGCAGA AAATAGCGGA AAAGTTCAGC CAGCGGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006235134.1
CDS	453..2534
Translation	MQLASGTMQRAMLLGLLGAAALAELFERTSTTICAVISAPVDNRDHNEEMVTRCIIEVLSNALSKSSAPTITPECRQVLRKSGKEVKGEEKGENENSKFEVRLLRDPSDASVGRWASSREETGAPVEDSPGQAKVDNEEWTGGGGHSREAVDDQESLHPSNQQVSKEAKIRHSEERGGKEEEEEEGKIYPKGEHRGDAGEEKKHTEESGEKHNAFSNKRSEASAKKKEESVARAEAHFVELEKTHSREQSSQESGEETRRQEKPQELPDQDQSEEESEEGEEGEEGATSEVTKRRPRHHHWRSQSNKPSYEGRRPLSEERKHAAGESKDANVATANLGEKRGHHLAHYRASEEEPDYGEELRSYPGFQAPQGLQYQGRGSEEVRAPSPRSEESQEKEYKRNHPDSELESTANRHSEETEEERSYEGAKGRQHRGRGREPGAYPALDSRQEKRLLDEGHDPVHESPVDTAKRYPQSKWQEQEKNYLNYDEEGDQGRWWQQEEQLEPEESREEVSFPDRQYAPYPTTEKRKRLGALFNPYFDPLQWKNSDFEKKGNPDDSFLDDDGEDGNGVTMTEKNFFPEYNYDWWEKRPFSEDVNWGYEKRSFARAPHLDLKRQYDDGVAELDQLLHYRKKAAEFPDFYDSEEQMGPHQEAEDEKDRADQRVLTEEEKKELENLAAMDLELQKIAEKFSQRG

Target ORF information:

RefSeq Version	XM_006235072.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus chromogranin B (Chgb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006235072.3

ORF Insert Sequence:

ATGCAGCTGG CGAGCGGCAC CATGCAGCGG GCCATGCTCC TCGGCCTCCT GGGCGCAGCA 
GCTCTGGCTG AGCTTTTTGA AAGGACATCC ACAACCATCT GTGCTGTCAT CTCAGCTCCA 
GTGGATAACA GGGATCACAA TGAAGAAATG GTGACTCGCT GCATTATTGA AGTCCTCTCA 
AATGCCCTAT CCAAGTCCAG TGCTCCAACC ATCACCCCTG AGTGCCGGCA AGTCCTGAGG 
AAGAGTGGAA AAGAGGTCAA AGGTGAAGAG AAAGGTGAAA ATGAGAACTC GAAGTTTGAA 
GTACGGTTGC TAAGAGACCC ATCTGATGCC TCAGTAGGCC GCTGGGCCTC CAGTAGGGAG 
GAAACAGGGG CTCCAGTAGA AGACTCTCCA GGTCAGGCGA AGGTAGACAA TGAGGAGTGG 
ACAGGAGGAG GAGGACACAG TCGAGAGGCA GTAGATGACC AGGAGAGCCT CCATCCCTCC 
AACCAACAAG TGTCCAAAGA AGCAAAGATA CGCCATTCGG AGGAGAGGGG CGGAAAAGAG 
GAGGAGGAGG AAGAAGGCAA GATTTATCCA AAGGGAGAGC ATAGGGGAGA TGCTGGTGAG 
GAGAAGAAAC ACACTGAAGA ATCTGGAGAG AAACACAACG CCTTCTCCAA CAAAAGAAGC 
GAAGCTTCAG CTAAGAAAAA AGAGGAGTCA GTGGCCAGAG CAGAGGCACA CTTCGTGGAG 
CTGGAGAAGA CACACAGCAG GGAGCAGAGC AGCCAGGAGA GTGGAGAGGA GACGAGGAGG 
CAGGAGAAAC CACAGGAGCT TCCCGACCAG GACCAGAGCG AGGAAGAATC CGAGGAAGGC 
GAGGAAGGCG AGGAAGGCGC AACCTCAGAA GTAACCAAAC GCAGGCCCAG GCACCACCAC 
TGGAGAAGCC AGTCCAACAA GCCCTCTTAC GAAGGGCGTC GTCCTCTGTC TGAGGAAAGG 
AAACATGCTG CTGGGGAGTC CAAGGATGCA AATGTGGCCA CAGCCAATTT AGGGGAAAAA 
AGGGGCCACC ACCTAGCTCA CTACCGGGCT TCAGAGGAAG AACCTGACTA TGGGGAAGAA 
TTGAGAAGCT ATCCGGGCTT CCAGGCTCCC CAGGGACTGC AGTACCAGGG CAGAGGAAGT 
GAAGAGGTCA GAGCCCCAAG CCCTCGGAGT GAGGAGAGCC AGGAAAAGGA GTACAAGAGA 
AACCACCCCG ACTCTGAACT GGAAAGCACA GCAAACAGAC ACAGTGAAGA AACTGAGGAA 
GAGAGGAGCT ACGAGGGGGC AAAGGGACGA CAACACAGAG GCAGGGGGCG GGAGCCAGGA 
GCTTATCCTG CTCTTGACAG CAGACAAGAA AAAAGGCTCC TGGATGAAGG GCATGACCCT 
GTTCATGAAA GCCCAGTAGA TACGGCAAAA AGGTATCCAC AAAGCAAATG GCAGGAACAG 
GAGAAAAACT ACCTCAACTA TGACGAGGAA GGGGACCAAG GAAGATGGTG GCAGCAGGAA 
GAGCAGCTAG AACCGGAAGA AAGCAGGGAG GAAGTGAGCT TTCCAGACAG ACAATACGCG 
CCCTACCCCA CCACTGAAAA GAGGAAGAGG CTAGGGGCGC TGTTCAACCC GTACTTTGAC 
CCTCTCCAGT GGAAGAATAG CGACTTTGAG AAAAAAGGCA ACCCAGATGA CAGTTTCCTT 
GATGATGACG GCGAAGACGG AAATGGGGTG ACAATGACTG AGAAGAATTT CTTCCCAGAG 
TACAACTATG ACTGGTGGGA GAAAAGGCCC TTCTCGGAGG ATGTGAATTG GGGATATGAG 
AAGAGAAGCT TTGCCAGGGC CCCTCACTTG GACTTGAAAA GGCAATACGA CGATGGAGTG 
GCTGAACTGG ACCAGCTCCT TCACTACAGG AAGAAGGCAG CCGAATTTCC CGACTTCTAC 
GATTCAGAGG AGCAGATGGG GCCTCACCAG GAGGCAGAAG ATGAAAAGGA CAGGGCTGAC 
CAGAGAGTTC TGACCGAGGA AGAGAAAAAG GAACTGGAGA ACTTGGCGGC CATGGATCTG 
GAACTGCAGA AAATAGCGGA AAAGTTCAGC CAGCGGGGCT GA