CXCL8 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	CXCL8
Entrez Gene ID	280828
Full Name	C-X-C motif chemokine ligand 8
Synonyms	IL-8,IL8
General protein information	Preferred Names C-X-C motif chemokine ligand 8 Names interleukin-8 C-X-C motif chemokine 8 chemokine (C-X-C motif) ligand 8 neutrophil activating peptide 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_173925.2	NP_776350.1	interleukin-8 precursor

Pathways from BioSystems

Homologies

	IL8	NP_001028137.1
Homo sapiens (human)	IL8	NP_000575.1
Pan troglodytes (chimpanzee)	IL8	XP_001156432.1
Canis lupus familiaris (dog)	IL8	NP_001003200.1
Bos taurus (cattle)	IL8	NP_776350.1

Gene Ontology
Bibliography

Related articles in PubMed

Molecular characterization of the bovine IER3 gene: Down-regulation of IL-8 by blocking NF-?B activity mediated by IER3 overexpression in MDBK cells infected with bovine viral diarrhea virus-1.
Villalba M, Fredericksen F, Otth C, Olavarr?a VH
Molecular immunology92169-179(2017 Dec)

Single Nucleotide Polymorphisms in IL8 and TLR4 Genes as Candidates for Digital Dermatitis Resistance/Susceptibility in Holstein Cattle.
El-Shafaey ES, Ateya A, Ramadan H, Saleh R, Elseady Y, Abo El Fadl E, El-Khodery S
Animal biotechnology28(2)131-137(2017 Apr)

In vivo relevance of polymorphic Interleukin 8 promoter haplotype for the systemic immune response to LPS in Holstein-Friesian calves.
Stojkovic B, McLoughlin RM, Meade KG
Veterinary immunology and immunopathology1821-10(2016 Dec)

Signal transducer and activator of transcription-3 licenses Toll-like receptor 4-dependent interleukin (IL)-6 and IL-8 production via IL-6 receptor-positive feedback in endometrial cells.
Cronin JG, Kanamarlapudi V, Thornton CA, Sheldon IM
Mucosal immunology9(5)1125-36(2016 09)

Genetic parameters estimated at receiving for circulating cortisol, immunoglobulin G, interleukin 8, and incidence of bovine respiratory disease in feedlot beef steers.
Cockrum RR, Speidel SE, Salak-Johnson JL, Chase CC, Peel RK, Weaber RL, Loneagan GH, Wagner JJ, Boddhireddy P, Thomas MG, Prayaga K, DeNise S, Enns RM
Journal of animal science94(7)2770-8(2016 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Molecular characterization of the bovine IER3 gene: Down-regulation of IL-8 by blocking NF-kappaB activity mediated by IER3 overexpression in MDBK cells infected with bovine viral diarrhea virus-1.
Title: Molecular characterization of the bovine IER3 gene: Down-regulation of IL-8 by blocking NF-?B activity mediated by IER3 overexpression in MDBK cells infected with bovine viral diarrhea virus-1.

As a pilot study, the present results revealed that identified SNPs in IL8 and TLR4 genes can be used as a genetic marker and predisposing factor for resistance/susceptibility to digital dermatitis in dairy cows. However, TLR4 gene may be a potential candidate for such disease.
Title: Single Nucleotide Polymorphisms in IL8 and TLR4 Genes as Candidates for Digital Dermatitis Resistance/Susceptibility in Holstein Cattle.

Genetic variation at the IL8 locus explains a proportion of the inter-breed and inter-individual variation in immunity between neonatal calves which is likely to influence their resistance to infection.
Title: In vivo relevance of polymorphic Interleukin 8 promoter haplotype for the systemic immune response to LPS in Holstein-Friesian calves.

STA3 facilitates TLR4-dependent IL-6 and IL-8 production via IL-6 receptor-positive feedback in endometrial cells.
Title: Signal transducer and activator of transcription-3 licenses Toll-like receptor 4-dependent interleukin (IL)-6 and IL-8 production via IL-6 receptor-positive feedback in endometrial cells.

Blood levels of cortisol, immunoglobulin G, and IL-8 in relationship to genetics and the incidence of bovine respiratory complex disease are reported.
Title: Genetic parameters estimated at receiving for circulating cortisol, immunoglobulin G, interleukin 8, and incidence of bovine respiratory disease in feedlot beef steers.

The following CXCL8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CXCL8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa01549	NM_173925.2 Latest version!	Bos taurus C-X-C motif chemokine ligand 8 (CXCL8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CXCL8 ( NM_173925.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776350.1 Bos taurus C-X-C motif chemokine ligand 8 (CXCL8), mRNA.

Price & Availability↑

CloneID	OBa01549
Clone ID Related Accession (Same CDS sequence)	NM_173925.2
Accession Version	NM_173925.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	interleukin-8 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF232704.1. On Jun 3, 2003 this sequence version replaced NM_173925.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF232704.1, DT808551.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145419, SAMN03145425 [ECO:0000348] ##Evidence-Data-END##

ATGACTTCCA AGCTGGCTGT TGCTCTCTTG GCAGCTTTCC TGCTCTCTGC AGCTCTGTGT 
GAAGCTGCAG TTCTGTCAAG AATGAGTACA GAACTTCGAT GCCAATGCAT AAAAACACAT 
TCCACACCTT TCCACCCCAA ATTTATCAAA GAATTGAGAG TTATTGAGAG TGGGCCACAC 
TGTGAAAATT CAGAAATCAT TGTTAAGCTT ACCAATGGAA ACGAGGTCTG CCTAAACCCC 
AAGGAAAAGT GGGTGCAGAA GGTTGTGCAG GTATTTGTGA AGAGAGCTGA GAAGCAAGAT 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776350.1
CDS	74..379
Translation	MTSKLAVALLAAFLLSAALCEAAVLSRMSTELRCQCIKTHSTPFHPKFIKELRVIESGPHCENSEIIVKLTNGNEVCLNPKEKWVQKVVQVFVKRAEKQDP

Target ORF information:

RefSeq Version	NM_173925.2
Organism	Bos taurus(cattle)
Definition	Bos taurus C-X-C motif chemokine ligand 8 (CXCL8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173925.2

ORF Insert Sequence:

ATGACTTCCA AGCTGGCTGT TGCTCTCTTG GCAGCTTTCC TGCTCTCTGC AGCTCTGTGT 
GAAGCTGCAG TTCTGTCAAG AATGAGTACA GAACTTCGAT GCCAATGCAT AAAAACACAT 
TCCACACCTT TCCACCCCAA ATTTATCAAA GAATTGAGAG TTATTGAGAG TGGGCCACAC 
TGTGAAAATT CAGAAATCAT TGTTAAGCTT ACCAATGGAA ACGAGGTCTG CCTAAACCCC 
AAGGAAAAGT GGGTGCAGAA GGTTGTGCAG GTATTTGTGA AGAGAGCTGA GAAGCAAGAT 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Molecular characterization of the bovine IER3 gene: Down-regulation of IL-8 by blocking NF-?B activity mediated by IER3 overexpression in MDBK cells infected with bovine viral diarrhea virus-1.
Molecular immunology92169-179(2017 Dec)
Villalba M,Fredericksen F,Otth C,Olavarr?a VH

Single Nucleotide Polymorphisms in IL8 and TLR4 Genes as Candidates for Digital Dermatitis Resistance/Susceptibility in Holstein Cattle.
Animal biotechnology28(2)131-137(2017 Apr)
El-Shafaey ES,Ateya A,Ramadan H,Saleh R,Elseady Y,Abo El Fadl E,El-Khodery S

In vivo relevance of polymorphic Interleukin 8 promoter haplotype for the systemic immune response to LPS in Holstein-Friesian calves.
Veterinary immunology and immunopathology1821-10(2016 Dec)
Stojkovic B,McLoughlin RM,Meade KG

Signal transducer and activator of transcription-3 licenses Toll-like receptor 4-dependent interleukin (IL)-6 and IL-8 production via IL-6 receptor-positive feedback in endometrial cells.
Mucosal immunology9(5)1125-36(2016 09)
Cronin JG,Kanamarlapudi V,Thornton CA,Sheldon IM

Genetic parameters estimated at receiving for circulating cortisol, immunoglobulin G, interleukin 8, and incidence of bovine respiratory disease in feedlot beef steers.
Journal of animal science94(7)2770-8(2016 Jul)
Cockrum RR,Speidel SE,Salak-Johnson JL,Chase CC,Peel RK,Weaber RL,Loneagan GH,Wagner JJ,Boddhireddy P,Thomas MG,Prayaga K,DeNise S,Enns RM