CXCL8 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	CXCL8
Entrez Gene ID	403850
Full Name	C-X-C motif chemokine ligand 8
Synonyms	IL8
General protein information	Preferred Names C-X-C motif chemokine ligand 8 Names interleukin-8 C-X-C motif chemokine 8 IL-8 chemokine (C-X-C motif) ligand 8
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001003200.1	NP_001003200.1	interleukin-8 precursor

Pathways from BioSystems

Homologies

	IL8	NP_001028137.1
Homo sapiens (human)	IL8	NP_000575.1
Pan troglodytes (chimpanzee)	IL8	XP_001156432.1
Canis lupus familiaris (dog)	IL8	NP_001003200.1
Bos taurus (cattle)	IL8	NP_776350.1

Gene Ontology
Bibliography

Related articles in PubMed

MCP-1, KC-like and IL-8 as critical mediators of pathogenesis caused by Babesia canis.
Gal?n A, Mayer I, Rafaj RB, Bendelja K, Su?i? V, Cer?n JJ, Mrljak V
PloS one13(1)e0190474(2018)

ERK2 and JNK1 contribute to TNF-?-induced IL-8 expression in synovial fibroblasts.
Namba S, Nakano R, Kitanaka T, Kitanaka N, Nakayama T, Sugiya H
PloS one12(8)e0182923(2017)

CC chemokine ligand 2 and CXC chemokine ligand 8 as neutrophil chemoattractant factors in canine idiopathic polyarthritis.
Murakami K, Maeda S, Yonezawa T, Matsuki N
Veterinary immunology and immunopathology18252-58(2016 Dec)

Assessment of CCL2 and CXCL8 chemokines in serum, bronchoalveolar lavage fluid and lung tissue samples from dogs affected with canine idiopathic pulmonary fibrosis.
Roels E, Krafft E, Farnir F, Holopainen S, Laurila HP, Rajam?ki MM, Day MJ, Antoine N, Pirottin D, Clercx C
Veterinary journal (London, England : 1997)206(1)75-82(2015 Oct)

Evaluation of chemokines CXCL8 and CCL2, serotonin, and vascular endothelial growth factor serum concentrations in healthy dogs from seven breeds with variable predisposition for canine idiopathic pulmonary fibrosis.
Roels E, Krafft E, Antoine N, Farnir F, Laurila HP, Holopainen S, Rajam?ki MM, Clercx C
Research in veterinary science10157-62(2015 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These observations indicate that the individual activation of ERK2 and JNK1 pathways contributes to TNF-alpha-induced IL-8 expression in synovial fibroblasts.
Title: ERK2 and JNK1 contribute to TNF-?-induced IL-8 expression in synovial fibroblasts.

data suggest that CCL2 acts as a PMNs chemotactic factor as well as CXCL8, and both CCL2 and CXCL8 facilitate the infiltration of PMNs into the joints of idiopathic polyarthritis
Title: CC chemokine ligand 2 and CXC chemokine ligand 8 as neutrophil chemoattractant factors in canine idiopathic polyarthritis.

The study findings suggest that both CCL2 and CXCL8 are involved in the pathogenesis of canine idiopathic pulmonary fibrosis.
Title: Assessment of CCL2 and CXCL8 chemokines in serum, bronchoalveolar lavage fluid and lung tissue samples from dogs affected with canine idiopathic pulmonary fibrosis.

High CXCL8 blood concentrations might be related to the breed predisposition of the West Highland white terrier for canine idiopathic pulmonary fibrosis.
Title: Evaluation of chemokines CXCL8 and CCL2, serotonin, and vascular endothelial growth factor serum concentrations in healthy dogs from seven breeds with variable predisposition for canine idiopathic pulmonary fibrosis.

hemangiosarcoma derived IL-8 may play a role in tumor development by modulating the tumor microenvironment
Title: Interleukin-8 promotes canine hemangiosarcoma growth by regulating the tumor microenvironment.

The following CXCL8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CXCL8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe00476	NM_001003200.1 Latest version!	Canis lupus familiaris C-X-C motif chemokine ligand 8 (CXCL8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CXCL8 ( NM_001003200.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001003200.1 Canis lupus familiaris C-X-C motif chemokine ligand 8 (CXCL8), mRNA.

Price & Availability↑

CloneID	OCe00476
Clone ID Related Accession (Same CDS sequence)	NM_001003200.1
Accession Version	NM_001003200.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Canis lupus familiaris(dog)
Product	interleukin-8 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U10308.1. On Jan 12, 2005 this sequence version replaced XM_532409.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U10308.1, DN382089.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END##

ATGACTTCCA AGCTGGCTGT TGCTCTCTTG GCAGCTTTTG TCCTTTCTGC AGCTCTCTGT 
GAAGCTGCAG TTCTGTCAAG AGTCAGTTCA GAACTTCGAT GCCAGTGTAT AAAAACACAC 
TCCACACCTT TCCATCCCAA ATATATTAAA GAACTGAGAG TGATTGACAG TGGCCCACAT 
TGTGAAAACT CAGAAATCAT TGTAAAGCTT TTCAATGGAA ATGAGGTGTG CCTGGACCCC 
AAGGAAAAAT GGGTACAAAA GGTTGTGCAG ATATTTCTAA AGAAGGCTGA GAAACAAGAT 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003200.1
CDS	7..312
Translation	MTSKLAVALLAAFVLSAALCEAAVLSRVSSELRCQCIKTHSTPFHPKYIKELRVIDSGPHCENSEIIVKLFNGNEVCLDPKEKWVQKVVQIFLKKAEKQDP

Target ORF information:

RefSeq Version	NM_001003200.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris C-X-C motif chemokine ligand 8 (CXCL8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003200.1

ORF Insert Sequence:

ATGACTTCCA AGCTGGCTGT TGCTCTCTTG GCAGCTTTTG TCCTTTCTGC AGCTCTCTGT 
GAAGCTGCAG TTCTGTCAAG AGTCAGTTCA GAACTTCGAT GCCAGTGTAT AAAAACACAC 
TCCACACCTT TCCATCCCAA ATATATTAAA GAACTGAGAG TGATTGACAG TGGCCCACAT 
TGTGAAAACT CAGAAATCAT TGTAAAGCTT TTCAATGGAA ATGAGGTGTG CCTGGACCCC 
AAGGAAAAAT GGGTACAAAA GGTTGTGCAG ATATTTCTAA AGAAGGCTGA GAAACAAGAT 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MCP-1, KC-like and IL-8 as critical mediators of pathogenesis caused by Babesia canis.
PloS one13(1)e0190474(2018)
Gal?n A,Mayer I,Rafaj RB,Bendelja K,Su?i? V,Cer?n JJ,Mrljak V

ERK2 and JNK1 contribute to TNF-?-induced IL-8 expression in synovial fibroblasts.
PloS one12(8)e0182923(2017)
Namba S,Nakano R,Kitanaka T,Kitanaka N,Nakayama T,Sugiya H

CC chemokine ligand 2 and CXC chemokine ligand 8 as neutrophil chemoattractant factors in canine idiopathic polyarthritis.
Veterinary immunology and immunopathology18252-58(2016 Dec)
Murakami K,Maeda S,Yonezawa T,Matsuki N

Assessment of CCL2 and CXCL8 chemokines in serum, bronchoalveolar lavage fluid and lung tissue samples from dogs affected with canine idiopathic pulmonary fibrosis.
Veterinary journal (London, England : 1997)206(1)75-82(2015 Oct)
Roels E,Krafft E,Farnir F,Holopainen S,Laurila HP,Rajam?ki MM,Day MJ,Antoine N,Pirottin D,Clercx C

Evaluation of chemokines CXCL8 and CCL2, serotonin, and vascular endothelial growth factor serum concentrations in healthy dogs from seven breeds with variable predisposition for canine idiopathic pulmonary fibrosis.
Research in veterinary science10157-62(2015 Aug)
Roels E,Krafft E,Antoine N,Farnir F,Laurila HP,Holopainen S,Rajam?ki MM,Clercx C