ROCK1 cDNA ORF clone, Bos indicus(Bos taurus indicus)

Gene
Clones
gRNAs

Summary

Gene Symbol	ROCK1
Entrez Gene ID	109577829
Full Name	Rho associated coiled-coil containing protein kinase 1
Gene Type	protein-coding
Organism	Bos indicus(Bos taurus indicus)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019986702.1	XP_019842261.1	rho-associated protein kinase 1 isoform X1
XM_019986704.1	XP_019842263.1	rho-associated protein kinase 1 isoform X2

Pathways from BioSystems
Gene Ontology

The following ROCK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ROCK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBo42590	XM_019986702.1 Latest version!	Bos indicus Rho associated coiled-coil containing protein kinase 1 (ROCK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBo42591	XM_019986704.1 Latest version!	Bos indicus Rho associated coiled-coil containing protein kinase 1 (ROCK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ROCK1 ( XM_019986702.1 ) cDNA ORF clone, Bos indicus(Bos taurus indicus) -> XP_019842261.1 Bos indicus Rho associated coiled-coil containing protein kinase 1 (ROCK1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBo42590
Clone ID Related Accession (Same CDS sequence)	XM_019986702.1
Accession Version	XM_019986702.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Bos indicus(Bos taurus indicus)
Product	rho-associated protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_032673.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos indicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGCTG AAGATTATGA AGTAGTGAAG GTGATTGGTA GAGGTGCATT TGGAGAAGTT 
CAATTGGTAA GGCATAAATC CACCAGGAAG GTGTACGCTA TGAAGCTCCT CAGCAAATTT 
GAAATGATTA AGAGATCTGA TTCTGCTTTT TTCTGGGAAG AAAGGGACAT CATGGCTTTT 
GCTAACAGTC CTTGGGTTGT TCAGCTGTTT TATGCATTCC AAGATGATCG TTATCTTTAC 
ATGGTAATGG AGTACATGCC TGGTGGAGAT CTTGTAAATT TAATGAGCAA CTATGACGTG 
CCTGAAAAAT GGGCCCGATT CTATACTGCA GAAGTAGTTC TTGCATTGGA TGCAATCCAT 
TCCATGGGTT TTATCCATAG AGATGTGAAG CCTGATAACA TGCTGCTGGA TAAATCTGGA 
CATTTGAAGT TAGCAGATTT TGGTACTTGT ATGAAGATGA ATAAGGAAGG CATGGTACGA 
TGTGATACAG CAGTTGGAAC ACCTGATTAT ATTTCCCCTG AAGTATTAAA ATCTCAAGGT 
GGCGATGGTT ATTATGGACG AGAATGTGAC TGGTGGTCAG TTGGGGTATT TTTATATGAA 
ATGCTTGTAG GTGATACACC ATTTTATGCA GATTCTCTGG TTGGAACTTA CAGTAAGATT 
ATGAACCATA AAAATTCTCT TACCTTCCCT GATGATAATG ACATATCAAA AGAAGCAAAA 
AATCTTATTT GTGCCTTCCT TACTGACAGA GAGGTGAGAT TAGGGCGAAA TGGTGTAGAA 
GAAATCAAAC GACATCTCTT CTTCAAAAAT GATCAATGGG CTTGGGAAAC ACTTCGAGAC 
ACTGTAGCAC CAGTTGTACC TGATTTAAGT AGTGACATAG ACACAAGTAA CTTTGATGAC 
TTGGAAGAAG ATAAAGGAGA TGAAGAAACA TTCCCTATAC CTAAAGCTTT TGTTGGCAAT 
CAACTACCTT TTGTAGGATT TACATATTAT AGCAATCGTA GATACTTATC TTCAGCAAAT 
CCTAACGATA ACAGAACTAG CTCTAATGTG GATAAAAGCT TGCAGGAAAA TTTGCAGAAA 
ACAATTTATA AGCTGGAAGA ACAGTTACAT AATGAAATRC AATTAAAAGA TGAAATGGAG 
CAGAAGTGCA GAACGTCAAA CATAAAACTA GACAAGATAA TGAAAGAATT GGACGAAGAG 
GGGAATCAAA GAAGAAATCT AGAATCTACA GTATCTCAGA TTGAGAAGGA GAAAATGTTG 
CTACAGCACA GAATTAATGA ATACCAAAGA AAAGCAGAAC AGGAAAATGA GAAGAGGAGG 
AATGTAGAAA ATGAAGTTTC TACATTAAAG GATCAGTTGG AAGACTTAAA GAAAGTCAGC 
CAGAATTCAC AGCTTGCTAA TGAGAAGCTG GCACAATTAC AAAAGCAGCT AGAAGAAGCC 
AATGACTTAC TTAGGACAGA ATCAGACACA GCGGTTAGAT TAAGGAAGAG TCACACAGAG 
ATGAGCAAAT CCATCACTCA GCTAGAGTCC CTAAACAGAG AACTGCAGGA GAGAAATAGA 
ATTTTAGAGA ATTCCAAGTC ACAAATGGAC AAAGATTATT ACCAACTACA GGCTGTATTA 
GAAGCTGAAC GAAGAGACAG AGGTCATGAT TCTGAGAAGA TTGGAGACCT ACAAGCTCGA 
ATTACATCTT TACAAGAGGA GGTGAAGCAT CTCAAACATA ATCTTGAAAG AGTGGAAGGA 
GAAAGAAAAG AGGCTCAAGA CATGCTTAAT CATTCAGAAA AGGAAAAGAA TAACTTAGAA 
ATAGATTTAA ACTACAAGCT TAAATCATTA CAGCAACGAT TAGAACAAGA GGTAAATGAA 
CATAAAGTAA CCAAAGCTCG TTTAACTGAC AAACATCAAT CTATTGAAGA GGCAAAGTCT 
GTTGCAATGT GTGAGATGGA AAAAAAGCTG AAGGAGGAGA GAGACGCTCG AGAGAAGGCT 
GAAAATAGGG TTGTTCAGAT TGAGAAGCAG TGCTCTATGC TAGATGTTGA TCTGAAGCAA 
TCTCAGCAGA AGCTGGAGCA TTTGATTGAA AATAAAGACA GGATGGAGGA TGAAGTTAAG 
AATCTAACGC TGCAGCTGGA GCAGGAATCA AACAAGCGAC TTTTGTTACA AAATGAATTG 
AAGACTCAAG CATTTGAGGC AGACAATTTA AAAGGTTTAG AAAAGCAGAT GAAACAGGAA 
ATAAATACTT TATTGGAAGC AAAGAGATTA TTGGAATTTG AATTAGCTCA GCTTACAAAA 
CAGTACAGAG GGAATGAAGG GCAGATGCGG GAGCTGCAGG ATCAGCTGGA AGCGGAGCAG 
TATTTCTCGA CACTTTATAA AACCCAGGTA AAGGAACTTA AGGAAGAAAT TGAAGAAAAA 
AACAGAGAAA ACTTAAAGAA AATACAGGAA CTACAAAGTG AAAAAGAAAC TCTTGCTACT 
CAGTTGGATC TAGCGGAAAC AAAGGCTGAA TCTGAGCAGT TGGCTCGAGG GCTTCTAGAA 
GAACAGTATT TTGAACTGAC CCAAGAAAGC AAGAAAGCTG CCTCAAGAAA TAGACAAGAG 
ATTACAGATA AAGATCACAC TCTTAGTCGG CTTGAAGAAA CGAATAGCAT GCTAACCAAA 
GATATTGAAT TATTAAGAAA AGAAAACGAA GAGCTAACTG ATAAAATGAG GAAGGCAGAG 
GAAGAATATA AATTGAAGAA GGAGGAGGAG ATCAATATCC TTAAAGCTGC CTTTGAAAAG 
AATATCAATA CAGAACGAAC CCTTAAAACA CAGGCTGTTA ACAAGTTGGC AGAAATAATG 
AATCGAAAGG ACTTTAAAAT TGATAGAAAG AAAGCAAATA CGCAGGATTT GAGAAAGAAA 
GAAAAAGAAA ATCGAAAGCT CCAACTTGAA CTCAACCAGG AAAGAGAGAA ATTCAACCAA 
ATGGTAGTGA AACATCAGAA GGAGCTGAAT GAAATGCAAG CGCAATTGGT AGAAGAATGT 
ACACATAAGA ATGAGCTTCA GATGCAGCTG GCCAGCAAGG AGAGTGACAT TGAGCAGCTG 
CGTGCCAAAC TGTTGGACCT TTCAGATTCT ACAAGTGTTG CTAGTTTTCC CAGTGCTGAT 
GAAACTGATG GAAACCTTCC AGAATCAAGA ATTGAAGGTT GGCTTTCAAT ACCAAATAGA 
GGAAATATCA AAAGATACGG CTGGAAGAAA CAGTATGTTG TGGTAAGCAG CAAAAAAATT 
TTGTTCTATA ATGATGAACA AGATAAGGAA CAATCCAATC CGTCTATGGT ATTGGACATT 
GATAAATTGT TTCATGTTCG TCCTGTAACA CAAGGAGATG TATACAGAGC TGAAACTGAA 
GAAATTCCTA AAATATTCCA GATACTGTAT GCAAATGAAG GTGAATGTAG AAAAGACTTA 
GAGGTGGAAC CAGTACAACA AGCTGAAAAA ACAAATTTTC AAAATCACAA AGGCCATGAG 
TTCATTCCTA CACTCTACCA CTTTCCCGCC AACTGTGAGG CCTGTGCCAA ACCTCTCTGG 
CATGTTTTTA AGCCACCCCC TGCCCTGGAG TGTCGAAGAT GCCATGTTAA GTGCCACAGA 
GACCATATAG ATAAGAAAGA GGACTTGATT TCTCCATGTA AAGTAAGTTA TGATGTAACA 
TCAGCAAGAG ATATGCTGCT GCTAGCGTGT TCTCAGGATG AACAAAAAAA ATGGGTAACT 
CATTTAGTAA AGAAGATCCC TAAGAATCCA CCATCTGGTT TTGTGCGTGC ATCCCCTCGA 
ACACTTTCTA CAAGGTCCAC TGCAAATCAG TCTTTCCGGA AAGTGGTCAA AAATACATCT 
GGAAAAGCTA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019842261.1
CDS	56..3910
Translation	MKAEDYEVVKVIGRGAFGEVQLVRHKSTRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWARFYTAEVVLALDAIHSMGFIHRDVKPDNMLLDKSGHLKLADFGTCMKMNKEGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNSLTFPDDNDISKEAKNLICAFLTDREVRLGRNGVEEIKRHLFFKNDQWAWETLRDTVAPVVPDLSSDIDTSNFDDLEEDKGDEETFPIPKAFVGNQLPFVGFTYYSNRRYLSSANPNDNRTSSNVDKSLQENLQKTIYKLEEQLHNEnullQLKDEMEQKCRTSNIKLDKIMKELDEEGNQRRNLESTVSQIEKEKMLLQHRINEYQRKAEQENEKRRNVENEVSTLKDQLEDLKKVSQNSQLANEKLAQLQKQLEEANDLLRTESDTAVRLRKSHTEMSKSITQLESLNRELQERNRILENSKSQMDKDYYQLQAVLEAERRDRGHDSEKIGDLQARITSLQEEVKHLKHNLERVEGERKEAQDMLNHSEKEKNNLEIDLNYKLKSLQQRLEQEVNEHKVTKARLTDKHQSIEEAKSVAMCEMEKKLKEERDAREKAENRVVQIEKQCSMLDVDLKQSQQKLEHLIENKDRMEDEVKNLTLQLEQESNKRLLLQNELKTQAFEADNLKGLEKQMKQEINTLLEAKRLLEFELAQLTKQYRGNEGQMRELQDQLEAEQYFSTLYKTQVKELKEEIEEKNRENLKKIQELQSEKETLATQLDLAETKAESEQLARGLLEEQYFELTQESKKAASRNRQEITDKDHTLSRLEETNSMLTKDIELLRKENEELTDKMRKAEEEYKLKKEEEINILKAAFEKNINTERTLKTQAVNKLAEIMNRKDFKIDRKKANTQDLRKKEKENRKLQLELNQEREKFNQMVVKHQKELNEMQAQLVEECTHKNELQMQLASKESDIEQLRAKLLDLSDSTSVASFPSADETDGNLPESRIEGWLSIPNRGNIKRYGWKKQYVVVSSKKILFYNDEQDKEQSNPSMVLDIDKLFHVRPVTQGDVYRAETEEIPKIFQILYANEGECRKDLEVEPVQQAEKTNFQNHKGHEFIPTLYHFPANCEACAKPLWHVFKPPPALECRRCHVKCHRDHIDKKEDLISPCKVSYDVTSARDMLLLACSQDEQKKWVTHLVKKIPKNPPSGFVRASPRTLSTRSTANQSFRKVVKNTSGKAS

Target ORF information:

RefSeq Version	XM_019986702.1
Organism	Bos indicus(Bos taurus indicus)
Definition	Bos indicus Rho associated coiled-coil containing protein kinase 1 (ROCK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019986702.1

ORF Insert Sequence:

ATGAAAGCTG AAGATTATGA AGTAGTGAAG GTGATTGGTA GAGGTGCATT TGGAGAAGTT 
CAATTGGTAA GGCATAAATC CACCAGGAAG GTGTACGCTA TGAAGCTCCT CAGCAAATTT 
GAAATGATTA AGAGATCTGA TTCTGCTTTT TTCTGGGAAG AAAGGGACAT CATGGCTTTT 
GCTAACAGTC CTTGGGTTGT TCAGCTGTTT TATGCATTCC AAGATGATCG TTATCTTTAC 
ATGGTAATGG AGTACATGCC TGGTGGAGAT CTTGTAAATT TAATGAGCAA CTATGACGTG 
CCTGAAAAAT GGGCCCGATT CTATACTGCA GAAGTAGTTC TTGCATTGGA TGCAATCCAT 
TCCATGGGTT TTATCCATAG AGATGTGAAG CCTGATAACA TGCTGCTGGA TAAATCTGGA 
CATTTGAAGT TAGCAGATTT TGGTACTTGT ATGAAGATGA ATAAGGAAGG CATGGTACGA 
TGTGATACAG CAGTTGGAAC ACCTGATTAT ATTTCCCCTG AAGTATTAAA ATCTCAAGGT 
GGCGATGGTT ATTATGGACG AGAATGTGAC TGGTGGTCAG TTGGGGTATT TTTATATGAA 
ATGCTTGTAG GTGATACACC ATTTTATGCA GATTCTCTGG TTGGAACTTA CAGTAAGATT 
ATGAACCATA AAAATTCTCT TACCTTCCCT GATGATAATG ACATATCAAA AGAAGCAAAA 
AATCTTATTT GTGCCTTCCT TACTGACAGA GAGGTGAGAT TAGGGCGAAA TGGTGTAGAA 
GAAATCAAAC GACATCTCTT CTTCAAAAAT GATCAATGGG CTTGGGAAAC ACTTCGAGAC 
ACTGTAGCAC CAGTTGTACC TGATTTAAGT AGTGACATAG ACACAAGTAA CTTTGATGAC 
TTGGAAGAAG ATAAAGGAGA TGAAGAAACA TTCCCTATAC CTAAAGCTTT TGTTGGCAAT 
CAACTACCTT TTGTAGGATT TACATATTAT AGCAATCGTA GATACTTATC TTCAGCAAAT 
CCTAACGATA ACAGAACTAG CTCTAATGTG GATAAAAGCT TGCAGGAAAA TTTGCAGAAA 
ACAATTTATA AGCTGGAAGA ACAGTTACAT AATGAAATRC AATTAAAAGA TGAAATGGAG 
CAGAAGTGCA GAACGTCAAA CATAAAACTA GACAAGATAA TGAAAGAATT GGACGAAGAG 
GGGAATCAAA GAAGAAATCT AGAATCTACA GTATCTCAGA TTGAGAAGGA GAAAATGTTG 
CTACAGCACA GAATTAATGA ATACCAAAGA AAAGCAGAAC AGGAAAATGA GAAGAGGAGG 
AATGTAGAAA ATGAAGTTTC TACATTAAAG GATCAGTTGG AAGACTTAAA GAAAGTCAGC 
CAGAATTCAC AGCTTGCTAA TGAGAAGCTG GCACAATTAC AAAAGCAGCT AGAAGAAGCC 
AATGACTTAC TTAGGACAGA ATCAGACACA GCGGTTAGAT TAAGGAAGAG TCACACAGAG 
ATGAGCAAAT CCATCACTCA GCTAGAGTCC CTAAACAGAG AACTGCAGGA GAGAAATAGA 
ATTTTAGAGA ATTCCAAGTC ACAAATGGAC AAAGATTATT ACCAACTACA GGCTGTATTA 
GAAGCTGAAC GAAGAGACAG AGGTCATGAT TCTGAGAAGA TTGGAGACCT ACAAGCTCGA 
ATTACATCTT TACAAGAGGA GGTGAAGCAT CTCAAACATA ATCTTGAAAG AGTGGAAGGA 
GAAAGAAAAG AGGCTCAAGA CATGCTTAAT CATTCAGAAA AGGAAAAGAA TAACTTAGAA 
ATAGATTTAA ACTACAAGCT TAAATCATTA CAGCAACGAT TAGAACAAGA GGTAAATGAA 
CATAAAGTAA CCAAAGCTCG TTTAACTGAC AAACATCAAT CTATTGAAGA GGCAAAGTCT 
GTTGCAATGT GTGAGATGGA AAAAAAGCTG AAGGAGGAGA GAGACGCTCG AGAGAAGGCT 
GAAAATAGGG TTGTTCAGAT TGAGAAGCAG TGCTCTATGC TAGATGTTGA TCTGAAGCAA 
TCTCAGCAGA AGCTGGAGCA TTTGATTGAA AATAAAGACA GGATGGAGGA TGAAGTTAAG 
AATCTAACGC TGCAGCTGGA GCAGGAATCA AACAAGCGAC TTTTGTTACA AAATGAATTG 
AAGACTCAAG CATTTGAGGC AGACAATTTA AAAGGTTTAG AAAAGCAGAT GAAACAGGAA 
ATAAATACTT TATTGGAAGC AAAGAGATTA TTGGAATTTG AATTAGCTCA GCTTACAAAA 
CAGTACAGAG GGAATGAAGG GCAGATGCGG GAGCTGCAGG ATCAGCTGGA AGCGGAGCAG 
TATTTCTCGA CACTTTATAA AACCCAGGTA AAGGAACTTA AGGAAGAAAT TGAAGAAAAA 
AACAGAGAAA ACTTAAAGAA AATACAGGAA CTACAAAGTG AAAAAGAAAC TCTTGCTACT 
CAGTTGGATC TAGCGGAAAC AAAGGCTGAA TCTGAGCAGT TGGCTCGAGG GCTTCTAGAA 
GAACAGTATT TTGAACTGAC CCAAGAAAGC AAGAAAGCTG CCTCAAGAAA TAGACAAGAG 
ATTACAGATA AAGATCACAC TCTTAGTCGG CTTGAAGAAA CGAATAGCAT GCTAACCAAA 
GATATTGAAT TATTAAGAAA AGAAAACGAA GAGCTAACTG ATAAAATGAG GAAGGCAGAG 
GAAGAATATA AATTGAAGAA GGAGGAGGAG ATCAATATCC TTAAAGCTGC CTTTGAAAAG 
AATATCAATA CAGAACGAAC CCTTAAAACA CAGGCTGTTA ACAAGTTGGC AGAAATAATG 
AATCGAAAGG ACTTTAAAAT TGATAGAAAG AAAGCAAATA CGCAGGATTT GAGAAAGAAA 
GAAAAAGAAA ATCGAAAGCT CCAACTTGAA CTCAACCAGG AAAGAGAGAA ATTCAACCAA 
ATGGTAGTGA AACATCAGAA GGAGCTGAAT GAAATGCAAG CGCAATTGGT AGAAGAATGT 
ACACATAAGA ATGAGCTTCA GATGCAGCTG GCCAGCAAGG AGAGTGACAT TGAGCAGCTG 
CGTGCCAAAC TGTTGGACCT TTCAGATTCT ACAAGTGTTG CTAGTTTTCC CAGTGCTGAT 
GAAACTGATG GAAACCTTCC AGAATCAAGA ATTGAAGGTT GGCTTTCAAT ACCAAATAGA 
GGAAATATCA AAAGATACGG CTGGAAGAAA CAGTATGTTG TGGTAAGCAG CAAAAAAATT 
TTGTTCTATA ATGATGAACA AGATAAGGAA CAATCCAATC CGTCTATGGT ATTGGACATT 
GATAAATTGT TTCATGTTCG TCCTGTAACA CAAGGAGATG TATACAGAGC TGAAACTGAA 
GAAATTCCTA AAATATTCCA GATACTGTAT GCAAATGAAG GTGAATGTAG AAAAGACTTA 
GAGGTGGAAC CAGTACAACA AGCTGAAAAA ACAAATTTTC AAAATCACAA AGGCCATGAG 
TTCATTCCTA CACTCTACCA CTTTCCCGCC AACTGTGAGG CCTGTGCCAA ACCTCTCTGG 
CATGTTTTTA AGCCACCCCC TGCCCTGGAG TGTCGAAGAT GCCATGTTAA GTGCCACAGA 
GACCATATAG ATAAGAAAGA GGACTTGATT TCTCCATGTA AAGTAAGTTA TGATGTAACA 
TCAGCAAGAG ATATGCTGCT GCTAGCGTGT TCTCAGGATG AACAAAAAAA ATGGGTAACT 
CATTTAGTAA AGAAGATCCC TAAGAATCCA CCATCTGGTT TTGTGCGTGC ATCCCCTCGA 
ACACTTTCTA CAAGGTCCAC TGCAAATCAG TCTTTCCGGA AAGTGGTCAA AAATACATCT 
GGAAAAGCTA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROCK1 ( XM_019986704.1 ) cDNA ORF clone, Bos indicus(Bos taurus indicus) -> XP_019842263.1 Bos indicus Rho associated coiled-coil containing protein kinase 1 (ROCK1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBo42591
Clone ID Related Accession (Same CDS sequence)	XM_019986704.1
Accession Version	XM_019986704.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Bos indicus(Bos taurus indicus)
Product	rho-associated protein kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_032673.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos indicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGCTG AAGATTATGA AGTAGTGAAG GTGATTGGTA GAGGTGCATT TGGAGAAGTT 
CAATTGGTAA GGCATAAATC CACCAGGAAG GTGTACGCTA TGAAGCTCCT CAGCAAATTT 
GAAATGATTA AGAGATCTGA TTCTGCTTTT TTCTGGGAAG AAAGGGACAT CATGGCTTTT 
GCTAACAGTC CTTGGGTTGT TCAGCTGTTT TATGCATTCC AAGATGATCG TTATCTTTAC 
ATGGTAATGG AGTACATGCC TGGTGGAGAT CTTGTAAATT TAATGAGCAA CTATGACGTG 
CCTGAAAAAT GGGCCCGATT CTATACTGCA GAAGTAGTTC TTGCATTGGA TGCAATCCAT 
TCCATGGGTT TTATCCATAG AGATGTGAAG CCTGATAACA TGCTGCTGGA TAAATCTGGA 
CATTTGAAGT TAGCAGATTT TGGTACTTGT ATGAAGATGA ATAAGGAAGG CATGGTACGA 
TGTGATACAG CAGTTGGAAC ACCTGATTAT ATTTCCCCTG AAGTATTAAA ATCTCAAGGT 
GGCGATGGTT ATTATGGACG AGAATGTGAC TGGTGGTCAG TTGGGGTATT TTTATATGAA 
ATGCTTGTAG GTGATACACC ATTTTATGCA GATTCTCTGG TTGGAACTTA CAGTAAGATT 
ATGAACCATA AAAATTCTCT TACCTTCCCT GATGATAATG ACATATCAAA AGAAGCAAAA 
AATCTTATTT GTGCCTTCCT TACTGACAGA GAGGTGAGAT TAGGGCGAAA TGGTGTAGAA 
GAAATCAAAC GACATCTCTT CTTCAAAAAT GATCAATGGG CTTGGGAAAC ACTTCGAGAC 
ACTGTAGCAC CAGTTGTACC TGATTTAAGT AGTGACATAG ACACAAGTAA CTTTGATGAC 
TTGGAAGAAG ATAAAGGAGA TGAAGAAACA TTCCCTATAC CTAAAGCTTT TGTTGGCAAT 
CAACTACCTT TTGTAGGATT TACATATTAT AGCAATCGTA GATACTTATC TTCAGCAAAT 
CCTAACGATA ACAGAACTAG CTCTAATGTG GATAAAAGCT TGCAGGAAAA TTTGCAGAAA 
ACAATTTATA AGCTGGAAGA ACAGTTACAT AATGAAATRC AATTAAAAGA TGAAATGGAG 
CAGAAGTGCA GAACGTCAAA CATAAAACTA GACAAGATAA TGAAAGAATT GGACGAAGAG 
GGGAATCAAA GAAGAAATCT AGAATCTACA GTATCTCAGA TTGAGAAGGA GAAAATGTTG 
CTACAGCACA GAATTAATGA ATACCAAAGA AAAGCAGAAC AGGAAAATGA GAAGAGGAGG 
AATGTAGAAA ATGAAGTTTC TACATTAAAG GATCAGTTGG AAGACTTAAA GAAAGTCAGC 
CAGAATTCAC AGCTTGCTAA TGAGAAGCTG GCACAATTAC AAAAGCAGCT AGAAGAAGCC 
AATGACTTAC TTAGGACAGA ATCAGACACA GCGGTTAGAT TAAGGAAGAG TCACACAGAG 
ATGAGCAAAT CCATCACTCA GCTAGAGTCC CTAAACAGAG AACTGCAGGA GAGAAATAGA 
ATTTTAGAGA ATTCCAAGTC ACAAATGGAC AAAGATTATT ACCAACTACA GGCTGTATTA 
GAAGCTGAAC GAAGAGACAG AGGTCATGAT TCTGAGAAGA TTGGAGACCT ACAAGCTCGA 
ATTACATCTT TACAAGAGGA GGTGAAGCAT CTCAAACATA ATCTTGAAAG AGTGGAAGGA 
GAAAGAAAAG AGGCTCAAGA CATGCTTAAT CATTCAGAAA AGGAAAAGAA TAACTTAGAA 
ATAGATTTAA ACTACAAGCT TAAATCATTA CAGCAACGAT TAGAACAAGA GGTAAATGAA 
CATAAAGTAA CCAAAGCTCG TTTAACTGAC AAACATCAAT CTATTGAAGA GGCAAAGTCT 
GTTGCAATGT GTGAGATGGA AAAAAAGCTG AAGGAGGAGA GAGACGCTCG AGAGAAGGCT 
GAAAATAGGG TTGTTCAGAT TGAGAAGCAG TGCTCTATGC TAGATGTTGA TCTGAAGCAA 
TCTCAGCAGA AGCTGGAGCA TTTGATTGAA AATAAAGACA GGATGGAGGA TGAAGTTAAG 
AATCTAACGC TGCAGCTGGA GCAGGAATCA AACAAGCGAC TTTTGTTACA AAATGAATTG 
AAGACTCAAG CATTTGAGGC AGACAATTTA AAAGGTTTAG AAAAGCAGAT GAAACAGGAA 
ATAAATACTT TATTGGAAGC AAAGAGATTA TTGGAATTTG AATTAGCTCA GCTTACAAAA 
CAGTACAGAG GGAATGAAGG GCAGATGCGG GAGCTGCAGG ATCAGCTGGA AGCGGAGCAG 
TATTTCTCGA CACTTTATAA AACCCAGGTA AAGGAACTTA AGGAAGAAAT TGAAGAAAAA 
AACAGAGAAA ACTTAAAGAA AATACAGGAA CTACAAAGTG AAAAAGAAAC TCTTGCTACT 
CAGTTGGATC TAGCGGAAAC AAAGGCTGAA TCTGAGCAGT TGGCTCGAGG GCTTCTAGAA 
GAACAGTATT TTGAACTGAC CCAAGAAAGC AAGAAAGCTG CCTCAAGAAA TAGACAAGAG 
ATTACAGATA AAGATCACAC TCTTAGTCGG CTTGAAGAAA CGAATAGCAT GCTAACCAAA 
GATATTGAAT TATTAAGAAA AGAAAACGAA GAGCTAACTG ATAAAATGAG GAAGGCAGAG 
GAAGAATATA AATTGAAGAA GGAGGAGGAG ATCAATATCC TTAAAGCTGC CTTTGAAAAG 
AATATCAATA CAGAACGAAC CCTTAAAACA CAGGCTGTTA ACAAGTTGGC AGAAATAATG 
AATCGAAAGG ACTTTAAAAT TGATAGAAAG AAAGCAAATA CGCAGGATTT GAGAAAGAAA 
GAAAAAGAAA ATCGAAAGCT CCAACTTGAA CTCAACCAGG AAAGAGAGAA ATTCAACCAA 
ATGGTAGTGA AACATCAGAA GGAGCTGAAT GAAATGCAAG CGCAATTGGT AGAAGAATGT 
ACACATAAGA ATGAGCTTCA GATGCAGCTG GCCAGCAAGG AGAGTGACAT TGAGCAGCTG 
CGTGCCAAAC TGTTGGACCT TTCAGATTCT ACAAGTGTTG CTAGTTTTCC CAGTGCTGAT 
GAAACTGATG GAAACCTTCC AGTTGGTCTG CCTGCATTCC TTATTTATTT ATCTTCTATT 
CTTGTCTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019842263.1
CDS	30..3221
Translation	MKAEDYEVVKVIGRGAFGEVQLVRHKSTRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWARFYTAEVVLALDAIHSMGFIHRDVKPDNMLLDKSGHLKLADFGTCMKMNKEGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNSLTFPDDNDISKEAKNLICAFLTDREVRLGRNGVEEIKRHLFFKNDQWAWETLRDTVAPVVPDLSSDIDTSNFDDLEEDKGDEETFPIPKAFVGNQLPFVGFTYYSNRRYLSSANPNDNRTSSNVDKSLQENLQKTIYKLEEQLHNEnullQLKDEMEQKCRTSNIKLDKIMKELDEEGNQRRNLESTVSQIEKEKMLLQHRINEYQRKAEQENEKRRNVENEVSTLKDQLEDLKKVSQNSQLANEKLAQLQKQLEEANDLLRTESDTAVRLRKSHTEMSKSITQLESLNRELQERNRILENSKSQMDKDYYQLQAVLEAERRDRGHDSEKIGDLQARITSLQEEVKHLKHNLERVEGERKEAQDMLNHSEKEKNNLEIDLNYKLKSLQQRLEQEVNEHKVTKARLTDKHQSIEEAKSVAMCEMEKKLKEERDAREKAENRVVQIEKQCSMLDVDLKQSQQKLEHLIENKDRMEDEVKNLTLQLEQESNKRLLLQNELKTQAFEADNLKGLEKQMKQEINTLLEAKRLLEFELAQLTKQYRGNEGQMRELQDQLEAEQYFSTLYKTQVKELKEEIEEKNRENLKKIQELQSEKETLATQLDLAETKAESEQLARGLLEEQYFELTQESKKAASRNRQEITDKDHTLSRLEETNSMLTKDIELLRKENEELTDKMRKAEEEYKLKKEEEINILKAAFEKNINTERTLKTQAVNKLAEIMNRKDFKIDRKKANTQDLRKKEKENRKLQLELNQEREKFNQMVVKHQKELNEMQAQLVEECTHKNELQMQLASKESDIEQLRAKLLDLSDSTSVASFPSADETDGNLPVGLPAFLIYLSSILVF

Target ORF information:

RefSeq Version	XM_019986704.1
Organism	Bos indicus(Bos taurus indicus)
Definition	Bos indicus Rho associated coiled-coil containing protein kinase 1 (ROCK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019986704.1

ORF Insert Sequence:

ATGAAAGCTG AAGATTATGA AGTAGTGAAG GTGATTGGTA GAGGTGCATT TGGAGAAGTT 
CAATTGGTAA GGCATAAATC CACCAGGAAG GTGTACGCTA TGAAGCTCCT CAGCAAATTT 
GAAATGATTA AGAGATCTGA TTCTGCTTTT TTCTGGGAAG AAAGGGACAT CATGGCTTTT 
GCTAACAGTC CTTGGGTTGT TCAGCTGTTT TATGCATTCC AAGATGATCG TTATCTTTAC 
ATGGTAATGG AGTACATGCC TGGTGGAGAT CTTGTAAATT TAATGAGCAA CTATGACGTG 
CCTGAAAAAT GGGCCCGATT CTATACTGCA GAAGTAGTTC TTGCATTGGA TGCAATCCAT 
TCCATGGGTT TTATCCATAG AGATGTGAAG CCTGATAACA TGCTGCTGGA TAAATCTGGA 
CATTTGAAGT TAGCAGATTT TGGTACTTGT ATGAAGATGA ATAAGGAAGG CATGGTACGA 
TGTGATACAG CAGTTGGAAC ACCTGATTAT ATTTCCCCTG AAGTATTAAA ATCTCAAGGT 
GGCGATGGTT ATTATGGACG AGAATGTGAC TGGTGGTCAG TTGGGGTATT TTTATATGAA 
ATGCTTGTAG GTGATACACC ATTTTATGCA GATTCTCTGG TTGGAACTTA CAGTAAGATT 
ATGAACCATA AAAATTCTCT TACCTTCCCT GATGATAATG ACATATCAAA AGAAGCAAAA 
AATCTTATTT GTGCCTTCCT TACTGACAGA GAGGTGAGAT TAGGGCGAAA TGGTGTAGAA 
GAAATCAAAC GACATCTCTT CTTCAAAAAT GATCAATGGG CTTGGGAAAC ACTTCGAGAC 
ACTGTAGCAC CAGTTGTACC TGATTTAAGT AGTGACATAG ACACAAGTAA CTTTGATGAC 
TTGGAAGAAG ATAAAGGAGA TGAAGAAACA TTCCCTATAC CTAAAGCTTT TGTTGGCAAT 
CAACTACCTT TTGTAGGATT TACATATTAT AGCAATCGTA GATACTTATC TTCAGCAAAT 
CCTAACGATA ACAGAACTAG CTCTAATGTG GATAAAAGCT TGCAGGAAAA TTTGCAGAAA 
ACAATTTATA AGCTGGAAGA ACAGTTACAT AATGAAATRC AATTAAAAGA TGAAATGGAG 
CAGAAGTGCA GAACGTCAAA CATAAAACTA GACAAGATAA TGAAAGAATT GGACGAAGAG 
GGGAATCAAA GAAGAAATCT AGAATCTACA GTATCTCAGA TTGAGAAGGA GAAAATGTTG 
CTACAGCACA GAATTAATGA ATACCAAAGA AAAGCAGAAC AGGAAAATGA GAAGAGGAGG 
AATGTAGAAA ATGAAGTTTC TACATTAAAG GATCAGTTGG AAGACTTAAA GAAAGTCAGC 
CAGAATTCAC AGCTTGCTAA TGAGAAGCTG GCACAATTAC AAAAGCAGCT AGAAGAAGCC 
AATGACTTAC TTAGGACAGA ATCAGACACA GCGGTTAGAT TAAGGAAGAG TCACACAGAG 
ATGAGCAAAT CCATCACTCA GCTAGAGTCC CTAAACAGAG AACTGCAGGA GAGAAATAGA 
ATTTTAGAGA ATTCCAAGTC ACAAATGGAC AAAGATTATT ACCAACTACA GGCTGTATTA 
GAAGCTGAAC GAAGAGACAG AGGTCATGAT TCTGAGAAGA TTGGAGACCT ACAAGCTCGA 
ATTACATCTT TACAAGAGGA GGTGAAGCAT CTCAAACATA ATCTTGAAAG AGTGGAAGGA 
GAAAGAAAAG AGGCTCAAGA CATGCTTAAT CATTCAGAAA AGGAAAAGAA TAACTTAGAA 
ATAGATTTAA ACTACAAGCT TAAATCATTA CAGCAACGAT TAGAACAAGA GGTAAATGAA 
CATAAAGTAA CCAAAGCTCG TTTAACTGAC AAACATCAAT CTATTGAAGA GGCAAAGTCT 
GTTGCAATGT GTGAGATGGA AAAAAAGCTG AAGGAGGAGA GAGACGCTCG AGAGAAGGCT 
GAAAATAGGG TTGTTCAGAT TGAGAAGCAG TGCTCTATGC TAGATGTTGA TCTGAAGCAA 
TCTCAGCAGA AGCTGGAGCA TTTGATTGAA AATAAAGACA GGATGGAGGA TGAAGTTAAG 
AATCTAACGC TGCAGCTGGA GCAGGAATCA AACAAGCGAC TTTTGTTACA AAATGAATTG 
AAGACTCAAG CATTTGAGGC AGACAATTTA AAAGGTTTAG AAAAGCAGAT GAAACAGGAA 
ATAAATACTT TATTGGAAGC AAAGAGATTA TTGGAATTTG AATTAGCTCA GCTTACAAAA 
CAGTACAGAG GGAATGAAGG GCAGATGCGG GAGCTGCAGG ATCAGCTGGA AGCGGAGCAG 
TATTTCTCGA CACTTTATAA AACCCAGGTA AAGGAACTTA AGGAAGAAAT TGAAGAAAAA 
AACAGAGAAA ACTTAAAGAA AATACAGGAA CTACAAAGTG AAAAAGAAAC TCTTGCTACT 
CAGTTGGATC TAGCGGAAAC AAAGGCTGAA TCTGAGCAGT TGGCTCGAGG GCTTCTAGAA 
GAACAGTATT TTGAACTGAC CCAAGAAAGC AAGAAAGCTG CCTCAAGAAA TAGACAAGAG 
ATTACAGATA AAGATCACAC TCTTAGTCGG CTTGAAGAAA CGAATAGCAT GCTAACCAAA 
GATATTGAAT TATTAAGAAA AGAAAACGAA GAGCTAACTG ATAAAATGAG GAAGGCAGAG 
GAAGAATATA AATTGAAGAA GGAGGAGGAG ATCAATATCC TTAAAGCTGC CTTTGAAAAG 
AATATCAATA CAGAACGAAC CCTTAAAACA CAGGCTGTTA ACAAGTTGGC AGAAATAATG 
AATCGAAAGG ACTTTAAAAT TGATAGAAAG AAAGCAAATA CGCAGGATTT GAGAAAGAAA 
GAAAAAGAAA ATCGAAAGCT CCAACTTGAA CTCAACCAGG AAAGAGAGAA ATTCAACCAA 
ATGGTAGTGA AACATCAGAA GGAGCTGAAT GAAATGCAAG CGCAATTGGT AGAAGAATGT 
ACACATAAGA ATGAGCTTCA GATGCAGCTG GCCAGCAAGG AGAGTGACAT TGAGCAGCTG 
CGTGCCAAAC TGTTGGACCT TTCAGATTCT ACAAGTGTTG CTAGTTTTCC CAGTGCTGAT 
GAAACTGATG GAAACCTTCC AGTTGGTCTG CCTGCATTCC TTATTTATTT ATCTTCTATT 
CTTGTCTTCT AA