EPAS1 cDNA ORF clone, Bos indicus(Bos taurus indicus)

Gene
Clones
gRNAs

Summary

Gene Symbol	EPAS1
Entrez Gene ID	109566045
Full Name	endothelial PAS domain protein 1
Gene Type	protein-coding
Organism	Bos indicus(Bos taurus indicus)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019970095.1	XP_019825654.1	endothelial PAS domain-containing protein 1 isoform X2
XM_019970094.1	XP_019825653.1	endothelial PAS domain-containing protein 1 isoform X1

Pathways from BioSystems
Gene Ontology

The following EPAS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EPAS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBo28953	XM_019970094.1 Latest version!	Bos indicus endothelial PAS domain protein 1 (EPAS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBo28954	XM_019970095.1 Latest version!	Bos indicus endothelial PAS domain protein 1 (EPAS1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EPAS1 ( XM_019970094.1 ) cDNA ORF clone, Bos indicus(Bos taurus indicus) -> XP_019825653.1 Bos indicus endothelial PAS domain protein 1 (EPAS1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBo28953
Clone ID Related Accession (Same CDS sequence)	XM_019970094.1
Accession Version	XM_019970094.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Bos indicus(Bos taurus indicus)
Product	endothelial PAS domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_032660.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos indicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGCTG ACAAGGAGAA GAAAAGTAGG AGCAGCTCTG AGAGGAGGAA GGAGAAGTCC 
CGAGATGCAG CCAGGTGCCG GCGGAGCAAG GAAACAGAGG TCTTCTATGA GCTGGCGCAC 
GAGCTGCCCC TGCCGCACAG CGTGAGCTCG CACCTGGACA AGGCCTCCAT CATGCGGCTG 
GCCATCAGCT TCCTGCGCAC GCACAAGCTC CTGTCCTCTG TTTGCTCTGA AAATGAATCT 
GAAGCCGACG CTGACCAGCA GATGGACAAC TTGTATCTGA AAGCCTTGGA GGGTTTCATT 
GCCGTGGTGA CCCAAGATGG TGACATGATC TTTCTGTCGG AGAACATCAG CAAGTTCATG 
GGACTCACAC AGGTAGAGTT AACAGGACAC AGTATCTTTG ACTTCACTCA TCCCTGTGAC 
CATGAGGAGA TCCGTGAGAA CTTGAGCCTC AAAAATGGCT CTGGTTTTGG GAAGAAAAGC 
AAAGACATGT CCACAGAGCG GGACTTCTTC ATGAGGATGA AGTGCACGGT CACCAACAGA 
GGCCGCACRG TCAACCTCAA GTCAGCCACC TGGAAGGTCT TGCACTGCAC GGGCCAGGTG 
AAGGTCTACA ACAACTGCCC CCCTCACAGC AGTCTCTGCG GCTGCAAGGA GCCGCTGCTG 
TCCTGCCTCA TCATCATGTG TGAGCCGATC CAGCACCCAT CCCACATGGA CATCCCACTG 
GACAGCAAGA CCTTCCTGAG CCGCCACAGT ATGGACATGA AGTTCACCTA CTGCGACGAC 
AGAATCACAG AACTGGTTGG TTACCACCCT GAAGAGCTGC TTGGCCGCTC AGCCTATGAG 
TTCTACCATG CACTGGACTC AGAGAACATG ACCAAAAGCC ACCAGAACTT GTGCACCAAG 
GGTCAGGTGG TGAGCGGCCA GTACCGGATG CTTGCCAAGC ACGGGGGCTA TGTGTGGCTG 
GAGACACAGG GGACGGTGAT CTACAACCCT CGCAACCTGC AGCCCCAGTG CATCATGTGT 
GTGAACTACG TCCTGAGTGA AATTGAGAAG AACGACGTGG TGTTCTCCAT GGATCAGACA 
GAGTCACCGT TTAAGCCGCA CCTGCTGACC ATGAACAGCA TCTTTGATAA CAGTGGCAAG 
GTGGCTGTGT CTGARAAGAG CAACTTCCTA TTCACCAAGC TGAAGGAGGA GCCCGAGGAG 
CTGGCCCAGC TGGCACCTAC CGCRGGAGAT ACCATCATTT CTCTGGATTT CGGGACCCCG 
AACTTTGAGG AGTCCTCAGC CTATGGCAAG GGCATCCTGC CCCCAGGCCA GCAGTGGACC 
GGAGAGGTGA AGAGCCACGG CACCCACAGT GAGGCCGGGA GCCTGCCCGC CTTCACTGTG 
CCCCAGGCGG CCGCCCTGGG GAACAGCACC CCCAGTGCCA GCAGCAGCAG CAGCTGCTCC 
ACGCCCAGTA GCCCTGGAGA CTATTACACA TCTCTAGATG ACAATCTGAA GATTGAAGCA 
ATCGAGAAGC TCTTTGCCAT GGACACAGAG GCGAAGGACC AGTGTGGCAC CCAGACTGAC 
TTTAACGAGC TGGATTTGGA GACCCTGGCA CCTTACATCC CCATGGACGG AGAGGACTTC 
CAGCTTAGCC CCATCTGCCC TGAGGAGAGC CTCCTGCCGG AGACCCCCCA GTCGGCCCCC 
CAGCACTGCT TCAGCACCAT GTCAAACATC TTCCAGCCAC TGGCTCCGAT GGCCTCTCAC 
AGCACCTTCC TCCTGGACAA GTATCAGCAG CAGCTGGAAA GCAAGAAGAC GGAGCCTGAG 
CAGCGGCGTG TGTCCTTCGC CTTCTTTGAC GGTGGGAGCA GGGTGTCCCT GCTGCAGTGC 
TGTGGTCAGA CCTACACCCC CCTCTCCTCC ATGGGGGGCA TTTCCAACAC CCAGTGGCCC 
CCTGACCCAC CACTACAGCT GGGGCCCACG AAGTGGCCTG GTGAAGACCG GCACGCAGAG 
GCCGTGGGGG CAGCGCCCCT GGGGCTCCCC CCCGCCACAC CCCATCTCGC CATGCTCAAG 
AAGAGGTCTG CCAAGGGCTT CGGGCCTCAG GGTCCAGACG TGATGAGCCC AGCCATGATT 
GCCCTCTCCA ACAAGCTGAA GCTGAAGCGA CAGCTGGAGT ACGAGGAGCA AGCCTTCCAA 
GACATGAGTG GGGGGGATCC GCCAGGCAGC AGCACCTCGC ATTTGATGTG GAARAGAATG 
AAGAGTCTCA GGGGTGGTGG GACCTGCTCT TTGATGCCGG ACAAGCTGCC AAATGCAAAT 
GTCCCTAATG ATGAGTTCAT CCAGAATCCC GTGAGGGGTA GGAGCCAGCC CCTGAGACAC 
TTATCACCRC CACAGCCTCC GTCTGCCACG AGCCCGGGGG AGCCCACCAA GAGTGGGTTC 
CCYGCTCAGT GTTACGCCCC CCAGTACCAG GACTACAGCC TACCCGCGGC TCACAAGATG 
TCAGGCATGG CAAGCCGGCT GCTCGGACCC TCGTTTGAGC CCTACCTGCT GCCGGAACTG 
ACCAGATATG ACTGTGAGGT GAACGTGCCT GTTCCGGGAA CCTCCACGCT CCTCCAAGGA 
GGGGATCTCC TCCGAGCCCT GGACCAGGCC ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019825653.1
CDS	571..3186
Translation	MTADKEKKSRSSSERRKEKSRDAARCRRSKETEVFYELAHELPLPHSVSSHLDKASIMRLAISFLRTHKLLSSVCSENESEADADQQMDNLYLKALEGFIAVVTQDGDMIFLSENISKFMGLTQVELTGHSIFDFTHPCDHEEIRENLSLKNGSGFGKKSKDMSTERDFFMRMKCTVTNRGRnullVNLKSATWKVLHCTGQVKVYNNCPPHSSLCGCKEPLLSCLIIMCEPIQHPSHMDIPLDSKTFLSRHSMDMKFTYCDDRITELVGYHPEELLGRSAYEFYHALDSENMTKSHQNLCTKGQVVSGQYRMLAKHGGYVWLETQGTVIYNPRNLQPQCIMCVNYVLSEIEKNDVVFSMDQTESPFKPHLLTMNSIFDNSGKVAVSnullKSNFLFTKLKEEPEELAQLAPTnullGDTIISLDFGTPNFEESSAYGKGILPPGQQWTGEVKSHGTHSEAGSLPAFTVPQAAALGNSTPSASSSSSCSTPSSPGDYYTSLDDNLKIEAIEKLFAMDTEAKDQCGTQTDFNELDLETLAPYIPMDGEDFQLSPICPEESLLPETPQSAPQHCFSTMSNIFQPLAPMASHSTFLLDKYQQQLESKKTEPEQRRVSFAFFDGGSRVSLLQCCGQTYTPLSSMGGISNTQWPPDPPLQLGPTKWPGEDRHAEAVGAAPLGLPPATPHLAMLKKRSAKGFGPQGPDVMSPAMIALSNKLKLKRQLEYEEQAFQDMSGGDPPGSSTSHLMWnullRMKSLRGGGTCSLMPDKLPNANVPNDEFIQNPVRGRSQPLRHLSnullPQPPSATSPGEPTKSGFnullAQCYAPQYQDYSLPAAHKMSGMASRLLGPSFEPYLLPELTRYDCEVNVPVPGTSTLLQGGDLLRALDQAT

Target ORF information:

RefSeq Version	XM_019970094.1
Organism	Bos indicus(Bos taurus indicus)
Definition	Bos indicus endothelial PAS domain protein 1 (EPAS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019970094.1

ORF Insert Sequence:

ATGACAGCTG ACAAGGAGAA GAAAAGTAGG AGCAGCTCTG AGAGGAGGAA GGAGAAGTCC 
CGAGATGCAG CCAGGTGCCG GCGGAGCAAG GAAACAGAGG TCTTCTATGA GCTGGCGCAC 
GAGCTGCCCC TGCCGCACAG CGTGAGCTCG CACCTGGACA AGGCCTCCAT CATGCGGCTG 
GCCATCAGCT TCCTGCGCAC GCACAAGCTC CTGTCCTCTG TTTGCTCTGA AAATGAATCT 
GAAGCCGACG CTGACCAGCA GATGGACAAC TTGTATCTGA AAGCCTTGGA GGGTTTCATT 
GCCGTGGTGA CCCAAGATGG TGACATGATC TTTCTGTCGG AGAACATCAG CAAGTTCATG 
GGACTCACAC AGGTAGAGTT AACAGGACAC AGTATCTTTG ACTTCACTCA TCCCTGTGAC 
CATGAGGAGA TCCGTGAGAA CTTGAGCCTC AAAAATGGCT CTGGTTTTGG GAAGAAAAGC 
AAAGACATGT CCACAGAGCG GGACTTCTTC ATGAGGATGA AGTGCACGGT CACCAACAGA 
GGCCGCACRG TCAACCTCAA GTCAGCCACC TGGAAGGTCT TGCACTGCAC GGGCCAGGTG 
AAGGTCTACA ACAACTGCCC CCCTCACAGC AGTCTCTGCG GCTGCAAGGA GCCGCTGCTG 
TCCTGCCTCA TCATCATGTG TGAGCCGATC CAGCACCCAT CCCACATGGA CATCCCACTG 
GACAGCAAGA CCTTCCTGAG CCGCCACAGT ATGGACATGA AGTTCACCTA CTGCGACGAC 
AGAATCACAG AACTGGTTGG TTACCACCCT GAAGAGCTGC TTGGCCGCTC AGCCTATGAG 
TTCTACCATG CACTGGACTC AGAGAACATG ACCAAAAGCC ACCAGAACTT GTGCACCAAG 
GGTCAGGTGG TGAGCGGCCA GTACCGGATG CTTGCCAAGC ACGGGGGCTA TGTGTGGCTG 
GAGACACAGG GGACGGTGAT CTACAACCCT CGCAACCTGC AGCCCCAGTG CATCATGTGT 
GTGAACTACG TCCTGAGTGA AATTGAGAAG AACGACGTGG TGTTCTCCAT GGATCAGACA 
GAGTCACCGT TTAAGCCGCA CCTGCTGACC ATGAACAGCA TCTTTGATAA CAGTGGCAAG 
GTGGCTGTGT CTGARAAGAG CAACTTCCTA TTCACCAAGC TGAAGGAGGA GCCCGAGGAG 
CTGGCCCAGC TGGCACCTAC CGCRGGAGAT ACCATCATTT CTCTGGATTT CGGGACCCCG 
AACTTTGAGG AGTCCTCAGC CTATGGCAAG GGCATCCTGC CCCCAGGCCA GCAGTGGACC 
GGAGAGGTGA AGAGCCACGG CACCCACAGT GAGGCCGGGA GCCTGCCCGC CTTCACTGTG 
CCCCAGGCGG CCGCCCTGGG GAACAGCACC CCCAGTGCCA GCAGCAGCAG CAGCTGCTCC 
ACGCCCAGTA GCCCTGGAGA CTATTACACA TCTCTAGATG ACAATCTGAA GATTGAAGCA 
ATCGAGAAGC TCTTTGCCAT GGACACAGAG GCGAAGGACC AGTGTGGCAC CCAGACTGAC 
TTTAACGAGC TGGATTTGGA GACCCTGGCA CCTTACATCC CCATGGACGG AGAGGACTTC 
CAGCTTAGCC CCATCTGCCC TGAGGAGAGC CTCCTGCCGG AGACCCCCCA GTCGGCCCCC 
CAGCACTGCT TCAGCACCAT GTCAAACATC TTCCAGCCAC TGGCTCCGAT GGCCTCTCAC 
AGCACCTTCC TCCTGGACAA GTATCAGCAG CAGCTGGAAA GCAAGAAGAC GGAGCCTGAG 
CAGCGGCGTG TGTCCTTCGC CTTCTTTGAC GGTGGGAGCA GGGTGTCCCT GCTGCAGTGC 
TGTGGTCAGA CCTACACCCC CCTCTCCTCC ATGGGGGGCA TTTCCAACAC CCAGTGGCCC 
CCTGACCCAC CACTACAGCT GGGGCCCACG AAGTGGCCTG GTGAAGACCG GCACGCAGAG 
GCCGTGGGGG CAGCGCCCCT GGGGCTCCCC CCCGCCACAC CCCATCTCGC CATGCTCAAG 
AAGAGGTCTG CCAAGGGCTT CGGGCCTCAG GGTCCAGACG TGATGAGCCC AGCCATGATT 
GCCCTCTCCA ACAAGCTGAA GCTGAAGCGA CAGCTGGAGT ACGAGGAGCA AGCCTTCCAA 
GACATGAGTG GGGGGGATCC GCCAGGCAGC AGCACCTCGC ATTTGATGTG GAARAGAATG 
AAGAGTCTCA GGGGTGGTGG GACCTGCTCT TTGATGCCGG ACAAGCTGCC AAATGCAAAT 
GTCCCTAATG ATGAGTTCAT CCAGAATCCC GTGAGGGGTA GGAGCCAGCC CCTGAGACAC 
TTATCACCRC CACAGCCTCC GTCTGCCACG AGCCCGGGGG AGCCCACCAA GAGTGGGTTC 
CCYGCTCAGT GTTACGCCCC CCAGTACCAG GACTACAGCC TACCCGCGGC TCACAAGATG 
TCAGGCATGG CAAGCCGGCT GCTCGGACCC TCGTTTGAGC CCTACCTGCT GCCGGAACTG 
ACCAGATATG ACTGTGAGGT GAACGTGCCT GTTCCGGGAA CCTCCACGCT CCTCCAAGGA 
GGGGATCTCC TCCGAGCCCT GGACCAGGCC ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EPAS1 ( XM_019970095.1 ) cDNA ORF clone, Bos indicus(Bos taurus indicus) -> XP_019825654.1 Bos indicus endothelial PAS domain protein 1 (EPAS1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBo28954
Clone ID Related Accession (Same CDS sequence)	XM_019970095.1
Accession Version	XM_019970095.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Bos indicus(Bos taurus indicus)
Product	endothelial PAS domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_032660.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos indicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGCTG ACAAGGAGAA GAAAAGGAGC AGCTCTGAGA GGAGGAAGGA GAAGTCCCGA 
GATGCAGCCA GGTGCCGGCG GAGCAAGGAA ACAGAGGTCT TCTATGAGCT GGCGCACGAG 
CTGCCCCTGC CGCACAGCGT GAGCTCGCAC CTGGACAAGG CCTCCATCAT GCGGCTGGCC 
ATCAGCTTCC TGCGCACGCA CAAGCTCCTG TCCTCTGTTT GCTCTGAAAA TGAATCTGAA 
GCCGACGCTG ACCAGCAGAT GGACAACTTG TATCTGAAAG CCTTGGAGGG TTTCATTGCC 
GTGGTGACCC AAGATGGTGA CATGATCTTT CTGTCGGAGA ACATCAGCAA GTTCATGGGA 
CTCACACAGG TAGAGTTAAC AGGACACAGT ATCTTTGACT TCACTCATCC CTGTGACCAT 
GAGGAGATCC GTGAGAACTT GAGCCTCAAA AATGGCTCTG GTTTTGGGAA GAAAAGCAAA 
GACATGTCCA CAGAGCGGGA CTTCTTCATG AGGATGAAGT GCACGGTCAC CAACAGAGGC 
CGCACRGTCA ACCTCAAGTC AGCCACCTGG AAGGTCTTGC ACTGCACGGG CCAGGTGAAG 
GTCTACAACA ACTGCCCCCC TCACAGCAGT CTCTGCGGCT GCAAGGAGCC GCTGCTGTCC 
TGCCTCATCA TCATGTGTGA GCCGATCCAG CACCCATCCC ACATGGACAT CCCACTGGAC 
AGCAAGACCT TCCTGAGCCG CCACAGTATG GACATGAAGT TCACCTACTG CGACGACAGA 
ATCACAGAAC TGGTTGGTTA CCACCCTGAA GAGCTGCTTG GCCGCTCAGC CTATGAGTTC 
TACCATGCAC TGGACTCAGA GAACATGACC AAAAGCCACC AGAACTTGTG CACCAAGGGT 
CAGGTGGTGA GCGGCCAGTA CCGGATGCTT GCCAAGCACG GGGGCTATGT GTGGCTGGAG 
ACACAGGGGA CGGTGATCTA CAACCCTCGC AACCTGCAGC CCCAGTGCAT CATGTGTGTG 
AACTACGTCC TGAGTGAAAT TGAGAAGAAC GACGTGGTGT TCTCCATGGA TCAGACAGAG 
TCACCGTTTA AGCCGCACCT GCTGACCATG AACAGCATCT TTGATAACAG TGGCAAGGTG 
GCTGTGTCTG ARAAGAGCAA CTTCCTATTC ACCAAGCTGA AGGAGGAGCC CGAGGAGCTG 
GCCCAGCTGG CACCTACCGC RGGAGATACC ATCATTTCTC TGGATTTCGG GACCCCGAAC 
TTTGAGGAGT CCTCAGCCTA TGGCAAGGGC ATCCTGCCCC CAGGCCAGCA GTGGACCGGA 
GAGGTGAAGA GCCACGGCAC CCACAGTGAG GCCGGGAGCC TGCCCGCCTT CACTGTGCCC 
CAGGCGGCCG CCCTGGGGAA CAGCACCCCC AGTGCCAGCA GCAGCAGCAG CTGCTCCACG 
CCCAGTAGCC CTGGAGACTA TTACACATCT CTAGATGACA ATCTGAAGAT TGAAGCAATC 
GAGAAGCTCT TTGCCATGGA CACAGAGGCG AAGGACCAGT GTGGCACCCA GACTGACTTT 
AACGAGCTGG ATTTGGAGAC CCTGGCACCT TACATCCCCA TGGACGGAGA GGACTTCCAG 
CTTAGCCCCA TCTGCCCTGA GGAGAGCCTC CTGCCGGAGA CCCCCCAGTC GGCCCCCCAG 
CACTGCTTCA GCACCATGTC AAACATCTTC CAGCCACTGG CTCCGATGGC CTCTCACAGC 
ACCTTCCTCC TGGACAAGTA TCAGCAGCAG CTGGAAAGCA AGAAGACGGA GCCTGAGCAG 
CGGCGTGTGT CCTTCGCCTT CTTTGACGGT GGGAGCAGGG TGTCCCTGCT GCAGTGCTGT 
GGTCAGACCT ACACCCCCCT CTCCTCCATG GGGGGCATTT CCAACACCCA GTGGCCCCCT 
GACCCACCAC TACAGCTGGG GCCCACGAAG TGGCCTGGTG AAGACCGGCA CGCAGAGGCC 
GTGGGGGCAG CGCCCCTGGG GCTCCCCCCC GCCACACCCC ATCTCGCCAT GCTCAAGAAG 
AGGTCTGCCA AGGGCTTCGG GCCTCAGGGT CCAGACGTGA TGAGCCCAGC CATGATTGCC 
CTCTCCAACA AGCTGAAGCT GAAGCGACAG CTGGAGTACG AGGAGCAAGC CTTCCAAGAC 
ATGAGTGGGG GGGATCCGCC AGGCAGCAGC ACCTCGCATT TGATGTGGAA RAGAATGAAG 
AGTCTCAGGG GTGGTGGGAC CTGCTCTTTG ATGCCGGACA AGCTGCCAAA TGCAAATGTC 
CCTAATGATG AGTTCATCCA GAATCCCGTG AGGGGTAGGA GCCAGCCCCT GAGACACTTA 
TCACCRCCAC AGCCTCCGTC TGCCACGAGC CCGGGGGAGC CCACCAAGAG TGGGTTCCCY 
GCTCAGTGTT ACGCCCCCCA GTACCAGGAC TACAGCCTAC CCGCGGCTCA CAAGATGTCA 
GGCATGGCAA GCCGGCTGCT CGGACCCTCG TTTGAGCCCT ACCTGCTGCC GGAACTGACC 
AGATATGACT GTGAGGTGAA CGTGCCTGTT CCGGGAACCT CCACGCTCCT CCAAGGAGGG 
GATCTCCTCC GAGCCCTGGA CCAGGCCACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019825654.1
CDS	274..2886
Translation	MTADKEKKRSSSERRKEKSRDAARCRRSKETEVFYELAHELPLPHSVSSHLDKASIMRLAISFLRTHKLLSSVCSENESEADADQQMDNLYLKALEGFIAVVTQDGDMIFLSENISKFMGLTQVELTGHSIFDFTHPCDHEEIRENLSLKNGSGFGKKSKDMSTERDFFMRMKCTVTNRGRnullVNLKSATWKVLHCTGQVKVYNNCPPHSSLCGCKEPLLSCLIIMCEPIQHPSHMDIPLDSKTFLSRHSMDMKFTYCDDRITELVGYHPEELLGRSAYEFYHALDSENMTKSHQNLCTKGQVVSGQYRMLAKHGGYVWLETQGTVIYNPRNLQPQCIMCVNYVLSEIEKNDVVFSMDQTESPFKPHLLTMNSIFDNSGKVAVSnullKSNFLFTKLKEEPEELAQLAPTnullGDTIISLDFGTPNFEESSAYGKGILPPGQQWTGEVKSHGTHSEAGSLPAFTVPQAAALGNSTPSASSSSSCSTPSSPGDYYTSLDDNLKIEAIEKLFAMDTEAKDQCGTQTDFNELDLETLAPYIPMDGEDFQLSPICPEESLLPETPQSAPQHCFSTMSNIFQPLAPMASHSTFLLDKYQQQLESKKTEPEQRRVSFAFFDGGSRVSLLQCCGQTYTPLSSMGGISNTQWPPDPPLQLGPTKWPGEDRHAEAVGAAPLGLPPATPHLAMLKKRSAKGFGPQGPDVMSPAMIALSNKLKLKRQLEYEEQAFQDMSGGDPPGSSTSHLMWnullRMKSLRGGGTCSLMPDKLPNANVPNDEFIQNPVRGRSQPLRHLSnullPQPPSATSPGEPTKSGFnullAQCYAPQYQDYSLPAAHKMSGMASRLLGPSFEPYLLPELTRYDCEVNVPVPGTSTLLQGGDLLRALDQAT

Target ORF information:

RefSeq Version	XM_019970095.1
Organism	Bos indicus(Bos taurus indicus)
Definition	Bos indicus endothelial PAS domain protein 1 (EPAS1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019970095.1

ORF Insert Sequence:

ATGACAGCTG ACAAGGAGAA GAAAAGGAGC AGCTCTGAGA GGAGGAAGGA GAAGTCCCGA 
GATGCAGCCA GGTGCCGGCG GAGCAAGGAA ACAGAGGTCT TCTATGAGCT GGCGCACGAG 
CTGCCCCTGC CGCACAGCGT GAGCTCGCAC CTGGACAAGG CCTCCATCAT GCGGCTGGCC 
ATCAGCTTCC TGCGCACGCA CAAGCTCCTG TCCTCTGTTT GCTCTGAAAA TGAATCTGAA 
GCCGACGCTG ACCAGCAGAT GGACAACTTG TATCTGAAAG CCTTGGAGGG TTTCATTGCC 
GTGGTGACCC AAGATGGTGA CATGATCTTT CTGTCGGAGA ACATCAGCAA GTTCATGGGA 
CTCACACAGG TAGAGTTAAC AGGACACAGT ATCTTTGACT TCACTCATCC CTGTGACCAT 
GAGGAGATCC GTGAGAACTT GAGCCTCAAA AATGGCTCTG GTTTTGGGAA GAAAAGCAAA 
GACATGTCCA CAGAGCGGGA CTTCTTCATG AGGATGAAGT GCACGGTCAC CAACAGAGGC 
CGCACRGTCA ACCTCAAGTC AGCCACCTGG AAGGTCTTGC ACTGCACGGG CCAGGTGAAG 
GTCTACAACA ACTGCCCCCC TCACAGCAGT CTCTGCGGCT GCAAGGAGCC GCTGCTGTCC 
TGCCTCATCA TCATGTGTGA GCCGATCCAG CACCCATCCC ACATGGACAT CCCACTGGAC 
AGCAAGACCT TCCTGAGCCG CCACAGTATG GACATGAAGT TCACCTACTG CGACGACAGA 
ATCACAGAAC TGGTTGGTTA CCACCCTGAA GAGCTGCTTG GCCGCTCAGC CTATGAGTTC 
TACCATGCAC TGGACTCAGA GAACATGACC AAAAGCCACC AGAACTTGTG CACCAAGGGT 
CAGGTGGTGA GCGGCCAGTA CCGGATGCTT GCCAAGCACG GGGGCTATGT GTGGCTGGAG 
ACACAGGGGA CGGTGATCTA CAACCCTCGC AACCTGCAGC CCCAGTGCAT CATGTGTGTG 
AACTACGTCC TGAGTGAAAT TGAGAAGAAC GACGTGGTGT TCTCCATGGA TCAGACAGAG 
TCACCGTTTA AGCCGCACCT GCTGACCATG AACAGCATCT TTGATAACAG TGGCAAGGTG 
GCTGTGTCTG ARAAGAGCAA CTTCCTATTC ACCAAGCTGA AGGAGGAGCC CGAGGAGCTG 
GCCCAGCTGG CACCTACCGC RGGAGATACC ATCATTTCTC TGGATTTCGG GACCCCGAAC 
TTTGAGGAGT CCTCAGCCTA TGGCAAGGGC ATCCTGCCCC CAGGCCAGCA GTGGACCGGA 
GAGGTGAAGA GCCACGGCAC CCACAGTGAG GCCGGGAGCC TGCCCGCCTT CACTGTGCCC 
CAGGCGGCCG CCCTGGGGAA CAGCACCCCC AGTGCCAGCA GCAGCAGCAG CTGCTCCACG 
CCCAGTAGCC CTGGAGACTA TTACACATCT CTAGATGACA ATCTGAAGAT TGAAGCAATC 
GAGAAGCTCT TTGCCATGGA CACAGAGGCG AAGGACCAGT GTGGCACCCA GACTGACTTT 
AACGAGCTGG ATTTGGAGAC CCTGGCACCT TACATCCCCA TGGACGGAGA GGACTTCCAG 
CTTAGCCCCA TCTGCCCTGA GGAGAGCCTC CTGCCGGAGA CCCCCCAGTC GGCCCCCCAG 
CACTGCTTCA GCACCATGTC AAACATCTTC CAGCCACTGG CTCCGATGGC CTCTCACAGC 
ACCTTCCTCC TGGACAAGTA TCAGCAGCAG CTGGAAAGCA AGAAGACGGA GCCTGAGCAG 
CGGCGTGTGT CCTTCGCCTT CTTTGACGGT GGGAGCAGGG TGTCCCTGCT GCAGTGCTGT 
GGTCAGACCT ACACCCCCCT CTCCTCCATG GGGGGCATTT CCAACACCCA GTGGCCCCCT 
GACCCACCAC TACAGCTGGG GCCCACGAAG TGGCCTGGTG AAGACCGGCA CGCAGAGGCC 
GTGGGGGCAG CGCCCCTGGG GCTCCCCCCC GCCACACCCC ATCTCGCCAT GCTCAAGAAG 
AGGTCTGCCA AGGGCTTCGG GCCTCAGGGT CCAGACGTGA TGAGCCCAGC CATGATTGCC 
CTCTCCAACA AGCTGAAGCT GAAGCGACAG CTGGAGTACG AGGAGCAAGC CTTCCAAGAC 
ATGAGTGGGG GGGATCCGCC AGGCAGCAGC ACCTCGCATT TGATGTGGAA RAGAATGAAG 
AGTCTCAGGG GTGGTGGGAC CTGCTCTTTG ATGCCGGACA AGCTGCCAAA TGCAAATGTC 
CCTAATGATG AGTTCATCCA GAATCCCGTG AGGGGTAGGA GCCAGCCCCT GAGACACTTA 
TCACCRCCAC AGCCTCCGTC TGCCACGAGC CCGGGGGAGC CCACCAAGAG TGGGTTCCCY 
GCTCAGTGTT ACGCCCCCCA GTACCAGGAC TACAGCCTAC CCGCGGCTCA CAAGATGTCA 
GGCATGGCAA GCCGGCTGCT CGGACCCTCG TTTGAGCCCT ACCTGCTGCC GGAACTGACC 
AGATATGACT GTGAGGTGAA CGTGCCTGTT CCGGGAACCT CCACGCTCCT CCAAGGAGGG 
GATCTCCTCC GAGCCCTGGA CCAGGCCACC TGA