DGKQ cDNA ORF clone, Bos indicus(Bos taurus indicus)

Gene
Clones
gRNAs

Summary

Gene Symbol	DGKQ
Entrez Gene ID	109560705
Full Name	diacylglycerol kinase theta
Gene Type	protein-coding
Organism	Bos indicus(Bos taurus indicus)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019963156.1	XP_019818715.1	diacylglycerol kinase theta isoform X1
XM_019963157.1	XP_019818716.1	diacylglycerol kinase theta isoform X2

Pathways from BioSystems
Gene Ontology

The following DGKQ gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DGKQ cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBo51358	XM_019963156.1 Latest version!	Bos indicus diacylglycerol kinase theta (DGKQ), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBo51359	XM_019963157.1 Latest version!	Bos indicus diacylglycerol kinase theta (DGKQ), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DGKQ ( XM_019963156.1 ) cDNA ORF clone, Bos indicus(Bos taurus indicus) -> XP_019818715.1 Bos indicus diacylglycerol kinase theta (DGKQ), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBo51358
Clone ID Related Accession (Same CDS sequence)	XM_019963156.1
Accession Version	XM_019963156.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Bos indicus(Bos taurus indicus)
Product	diacylglycerol kinase theta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_032655.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos indicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCCGAGCC CGGGGCCCGA GGCTGGCTTG GCGGCGGCTC CCCGCGCCCT 
GGCAGCCCGA CCTCTAGCCC GGAGCTGGGC GCCGGAAGCC GCTCGAGATC GGGGCCGGGG 
TCCGGGCCGG GGTCGGGGCC GGAGCGGTCG GGCTCCAGGC CCCCAGGGCC CGCCGCGCCG 
AGTCACAGCT TCAGGAAGGT GACGCTTACC AAGCCCACCT TCTGCCACCT CTGCTCCGAT 
TTCATCTGGG GGCTTGCCGG CATCCTGTGT GACGTTTGCA ACTTCATGTC CCATGAGAAG 
TGCCTGAAGC ACGTGAAGAC CCCCTGCACG AGCGTGGCTC CCAGCCTGGT CCGCGTCCCG 
GTGGCCCACT GCTTCGGCCC CCGGGGGCTC TACAAGCGCA GGTTCTGCTC CGTGTGCCGG 
AAGGGCCTGG AGGCGCCGGG CCTCCGCTGC GAAGTGTGTG AGCTGCACGT TCACCCCGAC 
TGTGTGCCCT TCGCCTGCAG CGACTGCCGC CAGTGCCACC AGGACGGGCA CCGCGATCAC 
GACGCGCACC TCCACCACTG GCGGGAGGGG ACCTTGCCGT CCGGCGCGCG TTGCGAGCTC 
TGTCGGAAGA CTTGCGGCTC CTCGGACGTG CCGGCCGGCG TGCGCTGCGA GTGGTGCGGC 
ATCCAGGCCC ACTCGGTCTG CTCCGCGGCG CTCGCCCCCG AGTGCACTTT CGGGCGCCTG 
CGCACCCTGG TCTTGCCCCC CGCCTGCGTG CGCCTCCTGT CCCGAAACTT TAGCAAGATG 
CACTGCTTTC GGATCCCCGA GAGCGCGGCG CCGGAGCCCG GGGACGGGGA GGACAGCGCC 
GACGGCAGCG TCCCCGCTGG CCCGGGCCGA GAGGTGGGGA CGCCTGAGTC CGGCAAGCAG 
ACTCTGAAGA TCTTTGACGG CAGCGACGCG ATGCAGCGAA ACCACTTCCG TGCAGTCACG 
GTCCCGCGCC TGGCCCGGAG CCAGGAGGTG CTGGAGGCGG CTCTGCGGGC TTACTACATC 
TCCGAGGACC CTCAGGGCTT CCAGCTGCAG GCACTCCCCA CGCCTGTGCA GCTTGGCGAC 
ACCGCGGCCG CGGGAAGGGT CTGGAGCGCT GGGCCTGCTG AGGAGGAGGG CGGCAGAGCG 
GCTCCCGAGG CCTGGGTCCT CCGTGCTCTG CCCCGCACCC AGGAGATCCT GAAGATCTAC 
CCGGCCTGGC TCAAGGTGGG TGTGGCCTAC GTGTCCATCC GTGTGACCCC GCAGAGCACT 
GCCCGCAGCG TGGTGCTGGA GGTCCTCCCG CTGCTCGGAC GGCAGGCCGA GGGGGCCGAG 
GGCTTCCAGC TGGTGGAGGT GCTCATGGGC AGCAGACAAG TCCAGCGCAC GGTGCTGGCG 
GACGAGGAGC CCTTGCTTGA CCGGCTTCGT GAGATCCGGC AGACGTCCCT GCGGCAGATG 
AGCCAGACGC GGTTCTACGT GGCTGAGAGC AGAGTGCCGG CCCCGCGCGT CTCTCTGTTT 
GTGGGCGGTC TGCCGCCAGG CCTGTCCCCC CAGGAGTACC GCAGGTTGCT GGATGAGGCC 
GTTACCAGCA AAGCTGCCCT GGTGACCGTG AGCCACGTGT ATTCCGCCCA AGGTGCCGTG 
GTGCTGGACG TGGCATGCTT TGCAGAGGCC GAGCGGCTGT ACATGCTGAT CAGGGACACA 
GCTGTGCGTG GCCGGCCACT GACTGCCCTG GTGCTCCCGG AGGTGCTGCA CACGAAGCTG 
GCCCCAGACT GCCGCCCCCT GCTCGTGTTT GTGAACCCCA GGAGTGGAGG ACTCAAGGGC 
CGCGACCTGC TCTGCAGTTT CCGGAAGCTG CTCAACCCCC ACCAGGTCTT TGAGCTGACC 
AACGGGGGGC CGCTGCCTGG GTTCCACGTG TTCTCCCGAG TGCCCTGCTT CCGGGTGCTG 
GTGTGCGGCG GGGACGGCAC TGTGGGCTGG GTACTCGCTG CCCTGGAGGA CCTGCGGCAC 
CACCTGGCCT GCCCGGAGCC TGCTGTAGCC ATCCTGCCCC TGGGCACAGG GAACGACCTG 
GGCCGGGTCC TGCGCTGGGG CGCGGGCTAC AGCGGGGAGG ACCCGTTGTC CGTGCTGCTG 
TCAGTGGACG AGGCGGACGN NGTGCTCGTG GACCGCTGGA CCATCCTGCT GGACGCTCAC 
GAGTCTGCCT GTGGGGAGGA CAGCGAGGCG GACGCAGAGC CCCCCAAGAT CGTGCAGATG 
AGTAACTACT GTGGGATCGG CATCGACGCA GAGCTGAGCC TGGACTTCCA CCAGGCGCGG 
GAGGAGGAGC CCGGCAAGTT CACAAGCAGG TTCCACAACA AGGGCGTGTA TGTGCGGGTC 
GGGCTGCAGA AGATCAGCCA CTCCCGCAGC CTGCACAGGG CCCTGCGGCT CCAGGTGGAG 
CAGCAGGAGG TGGAGCTGCC CAGCATCGAG GGGCTCATCT TCATCAACAT CCCCAGCTGG 
GGCTCAGGGG CCGACCTGTG GGGTTCCGAC AGCGACTCGA GGTTCGAGAA GCCGCGCATG 
GACGACGGGC TGCTGGAGGT GGTGGGGGTG ACGGGCGTTG TGCACATGGG CCAGGTCCAG 
AGCGGCCTGC GCTCGGGCAT CCGCATTGCC CAGGGGTCCT ACTTCCGCGT CACTCTCCTC 
AAGGCCACGC CCGTGCAGGT GGATGGTGAA CCCTGGGTCC AGGCTCCCGG GCACCTGATC 
ATCTCAGCTG CAGGCCCTAA GGTCCACATG CTCAGGAAGG CGAAGCAGAA GCCCAGGAAG 
GCGGGGCCCG CCAAGGATGC ACGAGCAGAT GGGGCGCCGG CCCCTGAGGG GGACCCCAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019818715.1
CDS	82..2904
Translation	MAAAAEPGARGWLGGGSPRPGSPTSSPELGAGSRSRSGPGSGPGSGPERSGSRPPGPAAPSHSFRKVTLTKPTFCHLCSDFIWGLAGILCDVCNFMSHEKCLKHVKTPCTSVAPSLVRVPVAHCFGPRGLYKRRFCSVCRKGLEAPGLRCEVCELHVHPDCVPFACSDCRQCHQDGHRDHDAHLHHWREGTLPSGARCELCRKTCGSSDVPAGVRCEWCGIQAHSVCSAALAPECTFGRLRTLVLPPACVRLLSRNFSKMHCFRIPESAAPEPGDGEDSADGSVPAGPGREVGTPESGKQTLKIFDGSDAMQRNHFRAVTVPRLARSQEVLEAALRAYYISEDPQGFQLQALPTPVQLGDTAAAGRVWSAGPAEEEGGRAAPEAWVLRALPRTQEILKIYPAWLKVGVAYVSIRVTPQSTARSVVLEVLPLLGRQAEGAEGFQLVEVLMGSRQVQRTVLADEEPLLDRLREIRQTSLRQMSQTRFYVAESRVPAPRVSLFVGGLPPGLSPQEYRRLLDEAVTSKAALVTVSHVYSAQGAVVLDVACFAEAERLYMLIRDTAVRGRPLTALVLPEVLHTKLAPDCRPLLVFVNPRSGGLKGRDLLCSFRKLLNPHQVFELTNGGPLPGFHVFSRVPCFRVLVCGGDGTVGWVLAALEDLRHHLACPEPAVAILPLGTGNDLGRVLRWGAGYSGEDPLSVLLSVDEADnullVLVDRWTILLDAHESACGEDSEADAEPPKIVQMSNYCGIGIDAELSLDFHQAREEEPGKFTSRFHNKGVYVRVGLQKISHSRSLHRALRLQVEQQEVELPSIEGLIFINIPSWGSGADLWGSDSDSRFEKPRMDDGLLEVVGVTGVVHMGQVQSGLRSGIRIAQGSYFRVTLLKATPVQVDGEPWVQAPGHLIISAAGPKVHMLRKAKQKPRKAGPAKDARADGAPAPEGDPK

Target ORF information:

RefSeq Version	XM_019963156.1
Organism	Bos indicus(Bos taurus indicus)
Definition	Bos indicus diacylglycerol kinase theta (DGKQ), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019963156.1

ORF Insert Sequence:

ATGGCGGCGG CGGCCGAGCC CGGGGCCCGA GGCTGGCTTG GCGGCGGCTC CCCGCGCCCT 
GGCAGCCCGA CCTCTAGCCC GGAGCTGGGC GCCGGAAGCC GCTCGAGATC GGGGCCGGGG 
TCCGGGCCGG GGTCGGGGCC GGAGCGGTCG GGCTCCAGGC CCCCAGGGCC CGCCGCGCCG 
AGTCACAGCT TCAGGAAGGT GACGCTTACC AAGCCCACCT TCTGCCACCT CTGCTCCGAT 
TTCATCTGGG GGCTTGCCGG CATCCTGTGT GACGTTTGCA ACTTCATGTC CCATGAGAAG 
TGCCTGAAGC ACGTGAAGAC CCCCTGCACG AGCGTGGCTC CCAGCCTGGT CCGCGTCCCG 
GTGGCCCACT GCTTCGGCCC CCGGGGGCTC TACAAGCGCA GGTTCTGCTC CGTGTGCCGG 
AAGGGCCTGG AGGCGCCGGG CCTCCGCTGC GAAGTGTGTG AGCTGCACGT TCACCCCGAC 
TGTGTGCCCT TCGCCTGCAG CGACTGCCGC CAGTGCCACC AGGACGGGCA CCGCGATCAC 
GACGCGCACC TCCACCACTG GCGGGAGGGG ACCTTGCCGT CCGGCGCGCG TTGCGAGCTC 
TGTCGGAAGA CTTGCGGCTC CTCGGACGTG CCGGCCGGCG TGCGCTGCGA GTGGTGCGGC 
ATCCAGGCCC ACTCGGTCTG CTCCGCGGCG CTCGCCCCCG AGTGCACTTT CGGGCGCCTG 
CGCACCCTGG TCTTGCCCCC CGCCTGCGTG CGCCTCCTGT CCCGAAACTT TAGCAAGATG 
CACTGCTTTC GGATCCCCGA GAGCGCGGCG CCGGAGCCCG GGGACGGGGA GGACAGCGCC 
GACGGCAGCG TCCCCGCTGG CCCGGGCCGA GAGGTGGGGA CGCCTGAGTC CGGCAAGCAG 
ACTCTGAAGA TCTTTGACGG CAGCGACGCG ATGCAGCGAA ACCACTTCCG TGCAGTCACG 
GTCCCGCGCC TGGCCCGGAG CCAGGAGGTG CTGGAGGCGG CTCTGCGGGC TTACTACATC 
TCCGAGGACC CTCAGGGCTT CCAGCTGCAG GCACTCCCCA CGCCTGTGCA GCTTGGCGAC 
ACCGCGGCCG CGGGAAGGGT CTGGAGCGCT GGGCCTGCTG AGGAGGAGGG CGGCAGAGCG 
GCTCCCGAGG CCTGGGTCCT CCGTGCTCTG CCCCGCACCC AGGAGATCCT GAAGATCTAC 
CCGGCCTGGC TCAAGGTGGG TGTGGCCTAC GTGTCCATCC GTGTGACCCC GCAGAGCACT 
GCCCGCAGCG TGGTGCTGGA GGTCCTCCCG CTGCTCGGAC GGCAGGCCGA GGGGGCCGAG 
GGCTTCCAGC TGGTGGAGGT GCTCATGGGC AGCAGACAAG TCCAGCGCAC GGTGCTGGCG 
GACGAGGAGC CCTTGCTTGA CCGGCTTCGT GAGATCCGGC AGACGTCCCT GCGGCAGATG 
AGCCAGACGC GGTTCTACGT GGCTGAGAGC AGAGTGCCGG CCCCGCGCGT CTCTCTGTTT 
GTGGGCGGTC TGCCGCCAGG CCTGTCCCCC CAGGAGTACC GCAGGTTGCT GGATGAGGCC 
GTTACCAGCA AAGCTGCCCT GGTGACCGTG AGCCACGTGT ATTCCGCCCA AGGTGCCGTG 
GTGCTGGACG TGGCATGCTT TGCAGAGGCC GAGCGGCTGT ACATGCTGAT CAGGGACACA 
GCTGTGCGTG GCCGGCCACT GACTGCCCTG GTGCTCCCGG AGGTGCTGCA CACGAAGCTG 
GCCCCAGACT GCCGCCCCCT GCTCGTGTTT GTGAACCCCA GGAGTGGAGG ACTCAAGGGC 
CGCGACCTGC TCTGCAGTTT CCGGAAGCTG CTCAACCCCC ACCAGGTCTT TGAGCTGACC 
AACGGGGGGC CGCTGCCTGG GTTCCACGTG TTCTCCCGAG TGCCCTGCTT CCGGGTGCTG 
GTGTGCGGCG GGGACGGCAC TGTGGGCTGG GTACTCGCTG CCCTGGAGGA CCTGCGGCAC 
CACCTGGCCT GCCCGGAGCC TGCTGTAGCC ATCCTGCCCC TGGGCACAGG GAACGACCTG 
GGCCGGGTCC TGCGCTGGGG CGCGGGCTAC AGCGGGGAGG ACCCGTTGTC CGTGCTGCTG 
TCAGTGGACG AGGCGGACGN NGTGCTCGTG GACCGCTGGA CCATCCTGCT GGACGCTCAC 
GAGTCTGCCT GTGGGGAGGA CAGCGAGGCG GACGCAGAGC CCCCCAAGAT CGTGCAGATG 
AGTAACTACT GTGGGATCGG CATCGACGCA GAGCTGAGCC TGGACTTCCA CCAGGCGCGG 
GAGGAGGAGC CCGGCAAGTT CACAAGCAGG TTCCACAACA AGGGCGTGTA TGTGCGGGTC 
GGGCTGCAGA AGATCAGCCA CTCCCGCAGC CTGCACAGGG CCCTGCGGCT CCAGGTGGAG 
CAGCAGGAGG TGGAGCTGCC CAGCATCGAG GGGCTCATCT TCATCAACAT CCCCAGCTGG 
GGCTCAGGGG CCGACCTGTG GGGTTCCGAC AGCGACTCGA GGTTCGAGAA GCCGCGCATG 
GACGACGGGC TGCTGGAGGT GGTGGGGGTG ACGGGCGTTG TGCACATGGG CCAGGTCCAG 
AGCGGCCTGC GCTCGGGCAT CCGCATTGCC CAGGGGTCCT ACTTCCGCGT CACTCTCCTC 
AAGGCCACGC CCGTGCAGGT GGATGGTGAA CCCTGGGTCC AGGCTCCCGG GCACCTGATC 
ATCTCAGCTG CAGGCCCTAA GGTCCACATG CTCAGGAAGG CGAAGCAGAA GCCCAGGAAG 
GCGGGGCCCG CCAAGGATGC ACGAGCAGAT GGGGCGCCGG CCCCTGAGGG GGACCCCAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKQ ( XM_019963157.1 ) cDNA ORF clone, Bos indicus(Bos taurus indicus) -> XP_019818716.1 Bos indicus diacylglycerol kinase theta (DGKQ), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBo51359
Clone ID Related Accession (Same CDS sequence)	XM_019963157.1
Accession Version	XM_019963157.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Bos indicus(Bos taurus indicus)
Product	diacylglycerol kinase theta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_032655.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos indicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCCGAGCC CGGGGCCCGA GGCTGGCTTG GCGGCGGCTC CCCGCGCCCT 
GGCAGCCCGA CCTCTAGCCC GGAGCTGGGC GCCGGAAGCC GCTCGAGATC GGGGCCGGGG 
TCCGGGCCGG GGTCGGGGCC GGAGCGGTCG GGCTCCAGGC CCCCAGGGCC CGCCGCGCCG 
AGTCACAGCT TCAGGAAGGT GACGCTTACC AAGCCCACCT TCTGCCACCT CTGCTCCGAT 
TTCATCTGGG GGCTTGCCGG CATCCTGTGT GACGTTTGCA ACTTCATGTC CCATGAGAAG 
TGCCTGAAGC ACGTGAAGAC CCCCTGCACG AGCGTGGCTC CCAGCCTGGT CCGCGTCCCG 
GTGGCCCACT GCTTCGGCCC CCGGGGGCTC TACAAGCGCA GGTTCTGCTC CGTGTGCCGG 
AAGGGCCTGG AGGCGCCGGG CCTCCGCTGC GAAGTGTGTG AGCTGCACGT TCACCCCGAC 
TGTGTGCCCT TCGCCTGCAG CGACTGCCGC CAGTGCCACC AGGACGGGCA CCGCGATCAC 
GACGCGCACC TCCACCACTG GCGGGAGGGG ACCTTGCCGT CCGGCGCGCG TTGCGAGCTC 
TGTCGGAAGA CTTGCGGCTC CTCGGACGTG CCGGCCGGCG TGCGCTGCGA GTGGTGCGGC 
ATCCAGGCCC ACTCGGTCTG CTCCGCGGCG CTCGCCCCCG AGTGCACTTT CGGGCGCCTG 
CGCACCCTGG TCTTGCCCCC CGCCTGCGTG CGCCTCCTGT CCCGAAACTT TAGCAAGATG 
CACTGCTTTC GGATCCCCGA GAGCGCGGCG CCGGAGCCCG GGGACGGGGA GGACAGCGCC 
GACGGCAGCG TCCCCGCTGG CCCGGGCCGA GAGGTGGGGA CGCCTGAGTC CGGCAAGCAG 
ACTCTGAAGA TCTTTGACGG CAGCGACGCG ATGCAGCGAA ACCACTTCCG TGCAGTCACG 
GTCCCGCGCC TGGCCCGGAG CCAGGAGGTG CTGGAGGCGG CTCTGCGGGC TTACTACATC 
TCCGAGGACC CTCAGGGCTT CCAGCTGCAG GCACTCCCCA CGCCTGTGCA GCTTGGCGAC 
ACCGCGGCCG CGGGAAGGGT CTGGAGCGCT GGGCCTGCTG AGGAGGAGGG CGGCAGAGCG 
GCTCCCGAGG CCTGGGTCCT CCGTGCTCTG CCCCGCACCC AGGAGATCCT GAAGATCTAC 
CCGGCCTGGC TCAAGGTGGG TGTGGCCTAC GTGTCCATCC GTGTGACCCC GCAGAGCACT 
GCCCGCAGCG TGGTGCTGGA GGTCCTCCCG CTGCTCGGAC GGCAGGCCGA GGGGGCCGAG 
GGCTTCCAGC TGGTGGAGGT GCTCATGGGC AGCAGACAAG TCCAGCGCAC GGTGCTGGCG 
GACGAGGAGC CCTTGCTTGA CCGGCTTCGT GAGATCCGGC AGACGTCCCT GCGGCAGATG 
AGCCAGACGC GGTTCTACGT GGCTGAGAGC AGAGTGCCGG CCCCGCGCGT CTCTCTGTTT 
GTGGGCGGTC TGCCGCCAGG CCTGTCCCCC CAGGAGTACC GCAGGTTGCT GGATGAGGCC 
GTTACCAGCA AAGCTGCCCT GGTGACCGTG AGCCACGTGT ATTCCGCCCA AGGTGCCGTG 
GTGCTGGACG TGGCATGCTT TGCAGAGGCC GAGCGGCTGT ACATGCTGAT CAGGGACACA 
GCTGTGCGTG GCCGGCCACT GACTGCCCTG GTGCTCCCGG AGGTGCTGCA CACGAAGCTG 
GCCCCAGACT GCCGCCCCCT GCTCGTGTTT GTGAACCCCA GGAGTGGAGG ACTCAAGGGC 
CGCGACCTGC TCTGCAGTTT CCGGAAGCTG CTCAACCCCC ACCAGGTCTT TGAGCTGACC 
AACGGGGGGC CGCTGCCTGG GTTCCACGTG TTCTCCCGAG TGCCCTGCTT CCGGGTGCTG 
GTGTGCGGCG GGGACGGCAC TGTGGGCTGG GTACTCGCTG CCCTGGAGGA CCTGCGGCAC 
CACCTGGCCT GCCCGGAGCC TGCTGTAGCC ATCCTGCCCC TGGGCACAGG GAACGACCTG 
GGCCGGGTCC TGCGCTGGGG CGCGGGCTAC AGCGGGGAGG ACCCGTTGTC CGTGCTGCTG 
TCAGTGGACG AGGCGGACGN NGTGCTCGTG GACCGCTGGA CCATCCTGCT GGACGCTCAC 
GAGTCTGCCT GTGGGGAGGA CAGCGAGGCG GACGCAGAGC CCCCCAAGAT CGTGCAGATG 
AGTAACTACT GTGGGATCGG CATCGACGCA GAGCTGAGCC TGGACTTCCA CCAGGCGCGG 
GAGGAGGAGC CCGGCAAGTT CACAAGCAGC TGGGGCTCAG GGGCCGACCT GTGGGGTTCC 
GACAGCGACT CGAGGTTCGA GAAGCCGCGC ATGGACGACG GGCTGCTGGA GGTGGTGGGG 
GTGACGGGCG TTGTGCACAT GGGCCAGGTC CAGAGCGGCC TGCGCTCGGG CATCCGCATT 
GCCCAGGGGT CCTACTTCCG CGTCACTCTC CTCAAGGCCA CGCCCGTGCA GGTGGATGGT 
GAACCCTGGG TCCAGGCTCC CGGGCACCTG ATCATCTCAG CTGCAGGCCC TAAGGTCCAC 
ATGCTCAGGA AGGCGAAGCA GAAGCCCAGG AAGGCGGGGC CCGCCAAGGA TGCACGAGCA 
GATGGGGCGC CGGCCCCTGA GGGGGACCCC AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019818716.1
CDS	84..2759
Translation	MAAAAEPGARGWLGGGSPRPGSPTSSPELGAGSRSRSGPGSGPGSGPERSGSRPPGPAAPSHSFRKVTLTKPTFCHLCSDFIWGLAGILCDVCNFMSHEKCLKHVKTPCTSVAPSLVRVPVAHCFGPRGLYKRRFCSVCRKGLEAPGLRCEVCELHVHPDCVPFACSDCRQCHQDGHRDHDAHLHHWREGTLPSGARCELCRKTCGSSDVPAGVRCEWCGIQAHSVCSAALAPECTFGRLRTLVLPPACVRLLSRNFSKMHCFRIPESAAPEPGDGEDSADGSVPAGPGREVGTPESGKQTLKIFDGSDAMQRNHFRAVTVPRLARSQEVLEAALRAYYISEDPQGFQLQALPTPVQLGDTAAAGRVWSAGPAEEEGGRAAPEAWVLRALPRTQEILKIYPAWLKVGVAYVSIRVTPQSTARSVVLEVLPLLGRQAEGAEGFQLVEVLMGSRQVQRTVLADEEPLLDRLREIRQTSLRQMSQTRFYVAESRVPAPRVSLFVGGLPPGLSPQEYRRLLDEAVTSKAALVTVSHVYSAQGAVVLDVACFAEAERLYMLIRDTAVRGRPLTALVLPEVLHTKLAPDCRPLLVFVNPRSGGLKGRDLLCSFRKLLNPHQVFELTNGGPLPGFHVFSRVPCFRVLVCGGDGTVGWVLAALEDLRHHLACPEPAVAILPLGTGNDLGRVLRWGAGYSGEDPLSVLLSVDEADnullVLVDRWTILLDAHESACGEDSEADAEPPKIVQMSNYCGIGIDAELSLDFHQAREEEPGKFTSSWGSGADLWGSDSDSRFEKPRMDDGLLEVVGVTGVVHMGQVQSGLRSGIRIAQGSYFRVTLLKATPVQVDGEPWVQAPGHLIISAAGPKVHMLRKAKQKPRKAGPAKDARADGAPAPEGDPK

Target ORF information:

RefSeq Version	XM_019963157.1
Organism	Bos indicus(Bos taurus indicus)
Definition	Bos indicus diacylglycerol kinase theta (DGKQ), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019963157.1

ORF Insert Sequence:

ATGGCGGCGG CGGCCGAGCC CGGGGCCCGA GGCTGGCTTG GCGGCGGCTC CCCGCGCCCT 
GGCAGCCCGA CCTCTAGCCC GGAGCTGGGC GCCGGAAGCC GCTCGAGATC GGGGCCGGGG 
TCCGGGCCGG GGTCGGGGCC GGAGCGGTCG GGCTCCAGGC CCCCAGGGCC CGCCGCGCCG 
AGTCACAGCT TCAGGAAGGT GACGCTTACC AAGCCCACCT TCTGCCACCT CTGCTCCGAT 
TTCATCTGGG GGCTTGCCGG CATCCTGTGT GACGTTTGCA ACTTCATGTC CCATGAGAAG 
TGCCTGAAGC ACGTGAAGAC CCCCTGCACG AGCGTGGCTC CCAGCCTGGT CCGCGTCCCG 
GTGGCCCACT GCTTCGGCCC CCGGGGGCTC TACAAGCGCA GGTTCTGCTC CGTGTGCCGG 
AAGGGCCTGG AGGCGCCGGG CCTCCGCTGC GAAGTGTGTG AGCTGCACGT TCACCCCGAC 
TGTGTGCCCT TCGCCTGCAG CGACTGCCGC CAGTGCCACC AGGACGGGCA CCGCGATCAC 
GACGCGCACC TCCACCACTG GCGGGAGGGG ACCTTGCCGT CCGGCGCGCG TTGCGAGCTC 
TGTCGGAAGA CTTGCGGCTC CTCGGACGTG CCGGCCGGCG TGCGCTGCGA GTGGTGCGGC 
ATCCAGGCCC ACTCGGTCTG CTCCGCGGCG CTCGCCCCCG AGTGCACTTT CGGGCGCCTG 
CGCACCCTGG TCTTGCCCCC CGCCTGCGTG CGCCTCCTGT CCCGAAACTT TAGCAAGATG 
CACTGCTTTC GGATCCCCGA GAGCGCGGCG CCGGAGCCCG GGGACGGGGA GGACAGCGCC 
GACGGCAGCG TCCCCGCTGG CCCGGGCCGA GAGGTGGGGA CGCCTGAGTC CGGCAAGCAG 
ACTCTGAAGA TCTTTGACGG CAGCGACGCG ATGCAGCGAA ACCACTTCCG TGCAGTCACG 
GTCCCGCGCC TGGCCCGGAG CCAGGAGGTG CTGGAGGCGG CTCTGCGGGC TTACTACATC 
TCCGAGGACC CTCAGGGCTT CCAGCTGCAG GCACTCCCCA CGCCTGTGCA GCTTGGCGAC 
ACCGCGGCCG CGGGAAGGGT CTGGAGCGCT GGGCCTGCTG AGGAGGAGGG CGGCAGAGCG 
GCTCCCGAGG CCTGGGTCCT CCGTGCTCTG CCCCGCACCC AGGAGATCCT GAAGATCTAC 
CCGGCCTGGC TCAAGGTGGG TGTGGCCTAC GTGTCCATCC GTGTGACCCC GCAGAGCACT 
GCCCGCAGCG TGGTGCTGGA GGTCCTCCCG CTGCTCGGAC GGCAGGCCGA GGGGGCCGAG 
GGCTTCCAGC TGGTGGAGGT GCTCATGGGC AGCAGACAAG TCCAGCGCAC GGTGCTGGCG 
GACGAGGAGC CCTTGCTTGA CCGGCTTCGT GAGATCCGGC AGACGTCCCT GCGGCAGATG 
AGCCAGACGC GGTTCTACGT GGCTGAGAGC AGAGTGCCGG CCCCGCGCGT CTCTCTGTTT 
GTGGGCGGTC TGCCGCCAGG CCTGTCCCCC CAGGAGTACC GCAGGTTGCT GGATGAGGCC 
GTTACCAGCA AAGCTGCCCT GGTGACCGTG AGCCACGTGT ATTCCGCCCA AGGTGCCGTG 
GTGCTGGACG TGGCATGCTT TGCAGAGGCC GAGCGGCTGT ACATGCTGAT CAGGGACACA 
GCTGTGCGTG GCCGGCCACT GACTGCCCTG GTGCTCCCGG AGGTGCTGCA CACGAAGCTG 
GCCCCAGACT GCCGCCCCCT GCTCGTGTTT GTGAACCCCA GGAGTGGAGG ACTCAAGGGC 
CGCGACCTGC TCTGCAGTTT CCGGAAGCTG CTCAACCCCC ACCAGGTCTT TGAGCTGACC 
AACGGGGGGC CGCTGCCTGG GTTCCACGTG TTCTCCCGAG TGCCCTGCTT CCGGGTGCTG 
GTGTGCGGCG GGGACGGCAC TGTGGGCTGG GTACTCGCTG CCCTGGAGGA CCTGCGGCAC 
CACCTGGCCT GCCCGGAGCC TGCTGTAGCC ATCCTGCCCC TGGGCACAGG GAACGACCTG 
GGCCGGGTCC TGCGCTGGGG CGCGGGCTAC AGCGGGGAGG ACCCGTTGTC CGTGCTGCTG 
TCAGTGGACG AGGCGGACGN NGTGCTCGTG GACCGCTGGA CCATCCTGCT GGACGCTCAC 
GAGTCTGCCT GTGGGGAGGA CAGCGAGGCG GACGCAGAGC CCCCCAAGAT CGTGCAGATG 
AGTAACTACT GTGGGATCGG CATCGACGCA GAGCTGAGCC TGGACTTCCA CCAGGCGCGG 
GAGGAGGAGC CCGGCAAGTT CACAAGCAGC TGGGGCTCAG GGGCCGACCT GTGGGGTTCC 
GACAGCGACT CGAGGTTCGA GAAGCCGCGC ATGGACGACG GGCTGCTGGA GGTGGTGGGG 
GTGACGGGCG TTGTGCACAT GGGCCAGGTC CAGAGCGGCC TGCGCTCGGG CATCCGCATT 
GCCCAGGGGT CCTACTTCCG CGTCACTCTC CTCAAGGCCA CGCCCGTGCA GGTGGATGGT 
GAACCCTGGG TCCAGGCTCC CGGGCACCTG ATCATCTCAG CTGCAGGCCC TAAGGTCCAC 
ATGCTCAGGA AGGCGAAGCA GAAGCCCAGG AAGGCGGGGC CCGCCAAGGA TGCACGAGCA 
GATGGGGCGC CGGCCCCTGA GGGGGACCCC AAGTAG