TWF1 cDNA ORF clone, Bos indicus(Bos taurus indicus)

Gene
Clones
gRNAs

Summary

Gene Symbol	TWF1
Entrez Gene ID	109559199
Full Name	twinfilin actin binding protein 1
Gene Type	protein-coding
Organism	Bos indicus(Bos taurus indicus)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019960754.1	XP_019816313.1	twinfilin-1

Pathways from BioSystems
Gene Ontology

The following TWF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TWF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBo49611	XM_019960754.1 Latest version!	Bos indicus twinfilin actin binding protein 1 (TWF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TWF1 ( XM_019960754.1 ) cDNA ORF clone, Bos indicus(Bos taurus indicus) -> XP_019816313.1 Bos indicus twinfilin actin binding protein 1 (TWF1), mRNA.

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CloneID	OBo49611
Clone ID Related Accession (Same CDS sequence)	XM_019960754.1
Accession Version	XM_019960754.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Bos indicus(Bos taurus indicus)
Product	twinfilin-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_032654.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos indicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGCGCC GGGGCCGGCG GAGGAGCGGC CACTTCCTGG AGCCGCCGGC CGGGGCCGCT 
CGCTGCACTC AGCGCTGGAG CCGGGAGCCC GCGGCCGCCG CCATGTCCCA CCAGACCGGC 
ATCCAAGCAA GTGAAGACGT TAAAGATATC TTTGCCAGAG CAAGAAATGG AAAATACAGA 
CTTCTGAAGA TATCTATTGA AAATGAGAAA CTTGTGATTG GGTCGTGTAG GAAGCCTTCA 
GATTCCTGGG ATCAGGATTA TGATTCCTTT GTTTTACCCC TGTTGGAGGA CAAACAACCG 
TGCTATGTAT TATTCAGGTT AGATTCTCAG AATGCCCAGG GATATGAATG GATATTCATT 
GCGTGGTCTC CTGATCATTC TCATGTTCGT CAGAAAATGT TGTATGCAGC AACAAGAGCA 
ACTCTGAAAA AGGAGTTTGG AGGTGGCCAC ATTAAAGACG AAATGTTTGG AACAGTAAAG 
GAAGATGTAT CATTACATGG GTATAAAAAA TATTTGCTGT CACAGTCTTC TCCTGCCCCA 
CTGACTGCAG CTGAAGAAGA ATTAAGACAG ATTAAAATTA ATGAGGTACA GACTGACGTG 
AGTGTGGACG CTAAGCATCA AACACTACAA GGAGTAGCAT TTCCTATTTC TCAAGAAGCT 
TTTCAAGCTT TGGAAAAATT GAATAACAGA CAGCTGAACT ATGTACAATT GGAAATAGAT 
ATAAAAAATG AAATCATAAT TTTGGCCAAC ACAATAAATA CAGAACTAAA AGATTTGCCA 
AAGAGGATTC CCAAAGATGC AGCACGTTAT CATTTCTTTC TGTATAAACA TTCTCATGAA 
GGAGACTATT TGGAGTCTAT AGTTTTTATT TATTCAATGC CTGGATACAC ATGCAGTATA 
AGAGAACGAA TGCTGTATTC TAGCTGCAAG AGCCCTCTGC TAGAGATTGT AGAAAGACAA 
CTACAAATGG ACATCATTAG AAAGATCGAG ATAGACAATG GGGATGAGTT GACTGCAGAC 
TTCCTTTATG AAGAAGTACA TCCCAAGCAG CATGCACATA AACAAAGTTT TGCAAAACCA 
AAAGGCCCTT CAGGAAAAAG AGGAATTCGA AGAATAATTA GGGGTCCAGC TGAAACTGAA 
GCTACTACTG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019816313.1
CDS	100..1254
Translation	MTRRGRRRSGHFLEPPAGAARCTQRWSREPAAAAMSHQTGIQASEDVKDIFARARNGKYRLLKISIENEKLVIGSCRKPSDSWDQDYDSFVLPLLEDKQPCYVLFRLDSQNAQGYEWIFIAWSPDHSHVRQKMLYAATRATLKKEFGGGHIKDEMFGTVKEDVSLHGYKKYLLSQSSPAPLTAAEEELRQIKINEVQTDVSVDAKHQTLQGVAFPISQEAFQALEKLNNRQLNYVQLEIDIKNEIIILANTINTELKDLPKRIPKDAARYHFFLYKHSHEGDYLESIVFIYSMPGYTCSIRERMLYSSCKSPLLEIVERQLQMDIIRKIEIDNGDELTADFLYEEVHPKQHAHKQSFAKPKGPSGKRGIRRIIRGPAETEATTE

Target ORF information:

RefSeq Version	XM_019960754.1
Organism	Bos indicus(Bos taurus indicus)
Definition	Bos indicus twinfilin actin binding protein 1 (TWF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019960754.1

ORF Insert Sequence:

ATGACGCGCC GGGGCCGGCG GAGGAGCGGC CACTTCCTGG AGCCGCCGGC CGGGGCCGCT 
CGCTGCACTC AGCGCTGGAG CCGGGAGCCC GCGGCCGCCG CCATGTCCCA CCAGACCGGC 
ATCCAAGCAA GTGAAGACGT TAAAGATATC TTTGCCAGAG CAAGAAATGG AAAATACAGA 
CTTCTGAAGA TATCTATTGA AAATGAGAAA CTTGTGATTG GGTCGTGTAG GAAGCCTTCA 
GATTCCTGGG ATCAGGATTA TGATTCCTTT GTTTTACCCC TGTTGGAGGA CAAACAACCG 
TGCTATGTAT TATTCAGGTT AGATTCTCAG AATGCCCAGG GATATGAATG GATATTCATT 
GCGTGGTCTC CTGATCATTC TCATGTTCGT CAGAAAATGT TGTATGCAGC AACAAGAGCA 
ACTCTGAAAA AGGAGTTTGG AGGTGGCCAC ATTAAAGACG AAATGTTTGG AACAGTAAAG 
GAAGATGTAT CATTACATGG GTATAAAAAA TATTTGCTGT CACAGTCTTC TCCTGCCCCA 
CTGACTGCAG CTGAAGAAGA ATTAAGACAG ATTAAAATTA ATGAGGTACA GACTGACGTG 
AGTGTGGACG CTAAGCATCA AACACTACAA GGAGTAGCAT TTCCTATTTC TCAAGAAGCT 
TTTCAAGCTT TGGAAAAATT GAATAACAGA CAGCTGAACT ATGTACAATT GGAAATAGAT 
ATAAAAAATG AAATCATAAT TTTGGCCAAC ACAATAAATA CAGAACTAAA AGATTTGCCA 
AAGAGGATTC CCAAAGATGC AGCACGTTAT CATTTCTTTC TGTATAAACA TTCTCATGAA 
GGAGACTATT TGGAGTCTAT AGTTTTTATT TATTCAATGC CTGGATACAC ATGCAGTATA 
AGAGAACGAA TGCTGTATTC TAGCTGCAAG AGCCCTCTGC TAGAGATTGT AGAAAGACAA 
CTACAAATGG ACATCATTAG AAAGATCGAG ATAGACAATG GGGATGAGTT GACTGCAGAC 
TTCCTTTATG AAGAAGTACA TCCCAAGCAG CATGCACATA AACAAAGTTT TGCAAAACCA 
AAAGGCCCTT CAGGAAAAAG AGGAATTCGA AGAATAATTA GGGGTCCAGC TGAAACTGAA 
GCTACTACTG AATAA