VPS50 cDNA ORF clone, Bos indicus(Bos taurus indicus)

Gene
Clones
gRNAs

Summary

Gene Symbol	VPS50
Entrez Gene ID	109558208
Full Name	VPS50, EARP/GARPII complex subunit
Gene Type	protein-coding
Organism	Bos indicus(Bos taurus indicus)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019959684.1	XP_019815243.1	syndetin isoform X1
XM_019959685.1	XP_019815244.1	syndetin isoform X2

Pathways from BioSystems
Gene Ontology

The following VPS50 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS50 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBo48817	XM_019959684.1 Latest version!	Bos indicus VPS50, EARP/GARPII complex subunit (VPS50), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBo48818	XM_019959685.1 Latest version!	Bos indicus VPS50, EARP/GARPII complex subunit (VPS50), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VPS50 ( XM_019959684.1 ) cDNA ORF clone, Bos indicus(Bos taurus indicus) -> XP_019815243.1 Bos indicus VPS50, EARP/GARPII complex subunit (VPS50), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBo48817
Clone ID Related Accession (Same CDS sequence)	XM_019959684.1
Accession Version	XM_019959684.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2895bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Bos indicus(Bos taurus indicus)
Product	syndetin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_032653.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos indicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAAAA TCAAATCTCT TATGACCCGG CAGGGTCTGA AAAGTCCTCC AGAGAGCTTC 
AGTGATCTTG GTGCCATTGA GAGTCTCCGG GTCCCTGGAA AGGAGGAATT CCGAGAACTT 
CGAGAACAGC CTAGTGACCC TCAAGCTGAA CAAGAACTAA TTAATAGTAT TGAACAAGTG 
TATTTTTCTA CAGATTCATT TGATATTGTT AAATATGAAC TGGAGAAGCT CCCTCCTGTT 
CTCAATTTGC AAGAATTAGA GGAGTACAGA GACAAATTGA AACAACAGCA AGCTGCAGTC 
TCAAAAAAAG TAGCAGATTT AATCCTTGAA AAACAGCCTG CTTATGTCAA GGAACTTGAA 
AGAGTTACCT CATTACAAAC AGGTCTTCAG TTAGCTGCTG TAATCTGCAC CAATGGGAGG 
AGACACTTGA ATATTGCAAA GGAAGGTTTT ACTCAAGCTA GTTTAGGCCT CCTTGCAAAT 
CAGAGGAAAC GTCAGTTGCT GATTGGACTT CTGAAATCTC TGAGAACGAT AAAAACATTG 
CAAAGAACAG ATGTCCGCCT AAGTGAAATG CTGGAGGAGG AAGACTATCC AGGAGCTATT 
CAGCTGTGCC TGGAATGTCA GAAAGCTGCC AGCACTTTTA AACATTACAG TTGCATAAGT 
GAACTGAATT CAAAGTTGCA AGATACTTTA GAACAAATTG AGGAACAGTT GGATGTTGCT 
CTGTCCAAAA TATGCAAGAA TTTTGACGTT AACCACTACA CCAAAGTTCA ACAAGCTTAT 
CGACTTCTTG GAAAAACACA GACAGCAATG GATCAACTTC ATATGCACTT CACCCAAGCC 
ATTCATAACA CCGTGTTTCA AGTTGTTCTT GGCTATGTGG AACTATGTGC AGGAAACACA 
GATACAAAAT TCCAAAAGCT GCAATATAAG GATCTCTGTA CGCATGTTAC ACCAGACAGC 
TATATYCCAT GCCTTGCTGA CCTGTGCAAA GCCCTGTGGG AAGTTATGCT CAGCTATTAC 
AGGACTATGG AATGGCATGA AAAGCATGAT AATGAGGATA CTACTTCTGC TTCTGAAGGG 
AGTAATATGA TGGGTGCTGA AGAGACAAGT TTTGATCGTG GCTACATAAA AAAGAAATTA 
GAACATGGAC TTACACGAAT ATGGCAGGAT GTTCAGCTAA AAGTTAAAAC CTACTTGCTT 
GGAACTGATT TGTCTATATT CAAATATGAT GATTTCATCT TTGTTTTGGA TATAATCAGC 
AGGTTGATGC AAGTTGGAGA AGAATTTTGT GGTAGCAAGT CTGAAGTTTT GCAAGAATCT 
ATTAGAAAAC AAAGTGTCAA TTATTTTAAG AACTACCATA GAACAAGGCT TGATGAACTG 
AGAATGTTTC TAGAGAATGA GACCTGGGAG CTTTGTCCTG TGAAGTCAAA TTTCAGCATC 
TTGCAACTTC ATGAATTTAA ATTCATGGAA CAGTCTCGTT CCCCATCGGT TTCACCCAGT 
AAGCAGTCAT CTGCAACTTC CTCAAAAACG GTAACCTTGT TTGAGCAGTA CTGCAGTGGT 
GGGAATCCAT TTGAAATTCA GGCGGACCAC AAAGATGAAG AAACAGAAGA TGTCCTAGCT 
TCTAATGGGT ATGAATCTGA TGAACAGGAA AAAAGTGCCT ATCAGGAATA TGACAGTGAC 
AGTGATGTTC CTGAGGAACT GAAACGAGAT TTCGTTGATG AGCAGACAGG CGATGCTCCT 
GTGAAAAGTG TTTCTCGAGA GACCCTAAAA AGCAGGAAGA AATCAGATTA CAGTCTAAAT 
AAAGTGAATG CACCCATCTT AACAAATACA ACATTGAATG TAATAAGGCT TGTTGGTAAA 
TATATGCAGA TGATGAACAT TCTTAAGCCA ATTGCTTTCG ATGTTATCCA TTTCATGTCT 
CAGCTCTTTG ATTATTACTT GTATGCAATA TATACCTTTT TTGGTCGGAA TGATTCATTG 
GAATCAACAG GACTAGGTCT TAGTAGTAGT AGACTAAGAA CAACTCTAAA CAGGATACAA 
GAAAGCCTCA TTGATCTGGA GGTTTCGGCT GATTCGGCTG CCACGCTCAC AGCAGCAGAA 
GAGAGGAAGG AGAAGGTGCC GAGTCCTCAT CTGAGTCACC TAGTGGTTTT GACCTCTGGG 
GACACACTGT ATGGGTTGGC GGAAAGAGTG GTCGCCACAG AATCCTTGGT ATTCTTGGCT 
GAACAGTTTG AGTTCCTTCA GCCTCATCTG GATGCTGTAA TGCCTGCAGT CAAAAAACCC 
TTTCTTCAGC AGTTTTATTC TCAGACGGTC TCAACGGCCA GTGAACTACG CAAACCAATT 
TACTGGATTG TAGCTGGTAA AGCCATTGAT TATGAACAGA TGCTGCTGCT GATGGCTAAT 
GTGAAATGGG ATGTAAAAGA AATTATGTCA CAGCACAACA TATATGTAGA TACGCTGTTA 
AAGGAATTTG AGCAGTTTAA CATGCGGTTG AATGAAGTTT CTAAGAGAGT TCGGATACCC 
TTGCCTGTGT CTAATATACT TTGGGAACAT TGTATACGCC TCGCTAATAG AACTATTGTG 
GAAGGATACG CCAACGTCAA GAAGTGTAGT AATGAGGGTC GTGCCTTGAT GCAATTGGAT 
TTTCAACAGT TTTTAATGAA ACTTGAAAAG CTAACTGATA TCAGACCCAT TCCGGATAAA 
GAATTTGTGG AAACCTATAT TAAAGCGTAC TACCTAACTG AGAATGACAT GGAACGCTGG 
ATCAAAGAGC ACAGGGAGTA TTCAACAAAG CAGCTGACCA ACCTGGTAAA TGTTTGCCTG 
GGATCCCATA TCAATAAGAA AGCAAGACAA AAACTTCTCT CAGCGATAGA TGATATAGAC 
AGACCCAAAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019815243.1
CDS	97..2991
Translation	MQKIKSLMTRQGLKSPPESFSDLGAIESLRVPGKEEFRELREQPSDPQAEQELINSIEQVYFSTDSFDIVKYELEKLPPVLNLQELEEYRDKLKQQQAAVSKKVADLILEKQPAYVKELERVTSLQTGLQLAAVICTNGRRHLNIAKEGFTQASLGLLANQRKRQLLIGLLKSLRTIKTLQRTDVRLSEMLEEEDYPGAIQLCLECQKAASTFKHYSCISELNSKLQDTLEQIEEQLDVALSKICKNFDVNHYTKVQQAYRLLGKTQTAMDQLHMHFTQAIHNTVFQVVLGYVELCAGNTDTKFQKLQYKDLCTHVTPDSYnullPCLADLCKALWEVMLSYYRTMEWHEKHDNEDTTSASEGSNMMGAEETSFDRGYIKKKLEHGLTRIWQDVQLKVKTYLLGTDLSIFKYDDFIFVLDIISRLMQVGEEFCGSKSEVLQESIRKQSVNYFKNYHRTRLDELRMFLENETWELCPVKSNFSILQLHEFKFMEQSRSPSVSPSKQSSATSSKTVTLFEQYCSGGNPFEIQADHKDEETEDVLASNGYESDEQEKSAYQEYDSDSDVPEELKRDFVDEQTGDAPVKSVSRETLKSRKKSDYSLNKVNAPILTNTTLNVIRLVGKYMQMMNILKPIAFDVIHFMSQLFDYYLYAIYTFFGRNDSLESTGLGLSSSRLRTTLNRIQESLIDLEVSADSAATLTAAEERKEKVPSPHLSHLVVLTSGDTLYGLAERVVATESLVFLAEQFEFLQPHLDAVMPAVKKPFLQQFYSQTVSTASELRKPIYWIVAGKAIDYEQMLLLMANVKWDVKEIMSQHNIYVDTLLKEFEQFNMRLNEVSKRVRIPLPVSNILWEHCIRLANRTIVEGYANVKKCSNEGRALMQLDFQQFLMKLEKLTDIRPIPDKEFVETYIKAYYLTENDMERWIKEHREYSTKQLTNLVNVCLGSHINKKARQKLLSAIDDIDRPKR

Target ORF information:

RefSeq Version	XM_019959684.1
Organism	Bos indicus(Bos taurus indicus)
Definition	Bos indicus VPS50, EARP/GARPII complex subunit (VPS50), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019959684.1

ORF Insert Sequence:

ATGCAAAAAA TCAAATCTCT TATGACCCGG CAGGGTCTGA AAAGTCCTCC AGAGAGCTTC 
AGTGATCTTG GTGCCATTGA GAGTCTCCGG GTCCCTGGAA AGGAGGAATT CCGAGAACTT 
CGAGAACAGC CTAGTGACCC TCAAGCTGAA CAAGAACTAA TTAATAGTAT TGAACAAGTG 
TATTTTTCTA CAGATTCATT TGATATTGTT AAATATGAAC TGGAGAAGCT CCCTCCTGTT 
CTCAATTTGC AAGAATTAGA GGAGTACAGA GACAAATTGA AACAACAGCA AGCTGCAGTC 
TCAAAAAAAG TAGCAGATTT AATCCTTGAA AAACAGCCTG CTTATGTCAA GGAACTTGAA 
AGAGTTACCT CATTACAAAC AGGTCTTCAG TTAGCTGCTG TAATCTGCAC CAATGGGAGG 
AGACACTTGA ATATTGCAAA GGAAGGTTTT ACTCAAGCTA GTTTAGGCCT CCTTGCAAAT 
CAGAGGAAAC GTCAGTTGCT GATTGGACTT CTGAAATCTC TGAGAACGAT AAAAACATTG 
CAAAGAACAG ATGTCCGCCT AAGTGAAATG CTGGAGGAGG AAGACTATCC AGGAGCTATT 
CAGCTGTGCC TGGAATGTCA GAAAGCTGCC AGCACTTTTA AACATTACAG TTGCATAAGT 
GAACTGAATT CAAAGTTGCA AGATACTTTA GAACAAATTG AGGAACAGTT GGATGTTGCT 
CTGTCCAAAA TATGCAAGAA TTTTGACGTT AACCACTACA CCAAAGTTCA ACAAGCTTAT 
CGACTTCTTG GAAAAACACA GACAGCAATG GATCAACTTC ATATGCACTT CACCCAAGCC 
ATTCATAACA CCGTGTTTCA AGTTGTTCTT GGCTATGTGG AACTATGTGC AGGAAACACA 
GATACAAAAT TCCAAAAGCT GCAATATAAG GATCTCTGTA CGCATGTTAC ACCAGACAGC 
TATATYCCAT GCCTTGCTGA CCTGTGCAAA GCCCTGTGGG AAGTTATGCT CAGCTATTAC 
AGGACTATGG AATGGCATGA AAAGCATGAT AATGAGGATA CTACTTCTGC TTCTGAAGGG 
AGTAATATGA TGGGTGCTGA AGAGACAAGT TTTGATCGTG GCTACATAAA AAAGAAATTA 
GAACATGGAC TTACACGAAT ATGGCAGGAT GTTCAGCTAA AAGTTAAAAC CTACTTGCTT 
GGAACTGATT TGTCTATATT CAAATATGAT GATTTCATCT TTGTTTTGGA TATAATCAGC 
AGGTTGATGC AAGTTGGAGA AGAATTTTGT GGTAGCAAGT CTGAAGTTTT GCAAGAATCT 
ATTAGAAAAC AAAGTGTCAA TTATTTTAAG AACTACCATA GAACAAGGCT TGATGAACTG 
AGAATGTTTC TAGAGAATGA GACCTGGGAG CTTTGTCCTG TGAAGTCAAA TTTCAGCATC 
TTGCAACTTC ATGAATTTAA ATTCATGGAA CAGTCTCGTT CCCCATCGGT TTCACCCAGT 
AAGCAGTCAT CTGCAACTTC CTCAAAAACG GTAACCTTGT TTGAGCAGTA CTGCAGTGGT 
GGGAATCCAT TTGAAATTCA GGCGGACCAC AAAGATGAAG AAACAGAAGA TGTCCTAGCT 
TCTAATGGGT ATGAATCTGA TGAACAGGAA AAAAGTGCCT ATCAGGAATA TGACAGTGAC 
AGTGATGTTC CTGAGGAACT GAAACGAGAT TTCGTTGATG AGCAGACAGG CGATGCTCCT 
GTGAAAAGTG TTTCTCGAGA GACCCTAAAA AGCAGGAAGA AATCAGATTA CAGTCTAAAT 
AAAGTGAATG CACCCATCTT AACAAATACA ACATTGAATG TAATAAGGCT TGTTGGTAAA 
TATATGCAGA TGATGAACAT TCTTAAGCCA ATTGCTTTCG ATGTTATCCA TTTCATGTCT 
CAGCTCTTTG ATTATTACTT GTATGCAATA TATACCTTTT TTGGTCGGAA TGATTCATTG 
GAATCAACAG GACTAGGTCT TAGTAGTAGT AGACTAAGAA CAACTCTAAA CAGGATACAA 
GAAAGCCTCA TTGATCTGGA GGTTTCGGCT GATTCGGCTG CCACGCTCAC AGCAGCAGAA 
GAGAGGAAGG AGAAGGTGCC GAGTCCTCAT CTGAGTCACC TAGTGGTTTT GACCTCTGGG 
GACACACTGT ATGGGTTGGC GGAAAGAGTG GTCGCCACAG AATCCTTGGT ATTCTTGGCT 
GAACAGTTTG AGTTCCTTCA GCCTCATCTG GATGCTGTAA TGCCTGCAGT CAAAAAACCC 
TTTCTTCAGC AGTTTTATTC TCAGACGGTC TCAACGGCCA GTGAACTACG CAAACCAATT 
TACTGGATTG TAGCTGGTAA AGCCATTGAT TATGAACAGA TGCTGCTGCT GATGGCTAAT 
GTGAAATGGG ATGTAAAAGA AATTATGTCA CAGCACAACA TATATGTAGA TACGCTGTTA 
AAGGAATTTG AGCAGTTTAA CATGCGGTTG AATGAAGTTT CTAAGAGAGT TCGGATACCC 
TTGCCTGTGT CTAATATACT TTGGGAACAT TGTATACGCC TCGCTAATAG AACTATTGTG 
GAAGGATACG CCAACGTCAA GAAGTGTAGT AATGAGGGTC GTGCCTTGAT GCAATTGGAT 
TTTCAACAGT TTTTAATGAA ACTTGAAAAG CTAACTGATA TCAGACCCAT TCCGGATAAA 
GAATTTGTGG AAACCTATAT TAAAGCGTAC TACCTAACTG AGAATGACAT GGAACGCTGG 
ATCAAAGAGC ACAGGGAGTA TTCAACAAAG CAGCTGACCA ACCTGGTAAA TGTTTGCCTG 
GGATCCCATA TCAATAAGAA AGCAAGACAA AAACTTCTCT CAGCGATAGA TGATATAGAC 
AGACCCAAAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS50 ( XM_019959685.1 ) cDNA ORF clone, Bos indicus(Bos taurus indicus) -> XP_019815244.1 Bos indicus VPS50, EARP/GARPII complex subunit (VPS50), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBo48818
Clone ID Related Accession (Same CDS sequence)	XM_019959685.1
Accession Version	XM_019959685.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Bos indicus(Bos taurus indicus)
Product	syndetin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_032653.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos indicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAAAA TCAAATCTCT TATGACCCGG CAGGGTCTGA AAAGTCCTCC AGAGAGCTTC 
AGTGATCTTG GTGCCATTGA GAGTCTCCGG GTCCCTGGAA AGAAGCTCCC TCCTGTTCTC 
AATTTGCAAG AATTAGAGGA GTACAGAGAC AAATTGAAAC AACAGCAAGC TGCAGTCTCA 
AAAAAAGTAG CAGATTTAAT CCTTGAAAAA CAGCCTGCTT ATGTCAAGGA ACTTGAAAGA 
GTTACCTCAT TACAAACAGG TCTTCAGTTA GCTGCTGTAA TCTGCACCAA TGGGAGGAGA 
CACTTGAATA TTGCAAAGGA AGGTTTTACT CAAGCTAGTT TAGGCCTCCT TGCAAATCAG 
AGGAAACGTC AGTTGCTGAT TGGACTTCTG AAATCTCTGA GAACGATAAA AACATTGCAA 
AGAACAGATG TCCGCCTAAG TGAAATGCTG GAGGAGGAAG ACTATCCAGG AGCTATTCAG 
CTGTGCCTGG AATGTCAGAA AGCTGCCAGC ACTTTTAAAC ATTACAGTTG CATAAGTGAA 
CTGAATTCAA AGTTGCAAGA TACTTTAGAA CAAATTGAGG AACAGTTGGA TGTTGCTCTG 
TCCAAAATAT GCAAGAATTT TGACGTTAAC CACTACACCA AAGTTCAACA AGCTTATCGA 
CTTCTTGGAA AAACACAGAC AGCAATGGAT CAACTTCATA TGCACTTCAC CCAAGCCATT 
CATAACACCG TGTTTCAAGT TGTTCTTGGC TATGTGGAAC TATGTGCAGG AAACACAGAT 
ACAAAATTCC AAAAGCTGCA ATATAAGGAT CTCTGTACGC ATGTTACACC AGACAGCTAT 
ATYCCATGCC TTGCTGACCT GTGCAAAGCC CTGTGGGAAG TTATGCTCAG CTATTACAGG 
ACTATGGAAT GGCATGAAAA GCATGATAAT GAGGATACTA CTTCTGCTTC TGAAGGGAGT 
AATATGATGG GTGCTGAAGA GACAAGTTTT GATCGTGGCT ACATAAAAAA GAAATTAGAA 
CATGGACTTA CACGAATATG GCAGGATGTT CAGCTAAAAG TTAAAACCTA CTTGCTTGGA 
ACTGATTTGT CTATATTCAA ATATGATGAT TTCATCTTTG TTTTGGATAT AATCAGCAGG 
TTGATGCAAG TTGGAGAAGA ATTTTGTGGT AGCAAGTCTG AAGTTTTGCA AGAATCTATT 
AGAAAACAAA GTGTCAATTA TTTTAAGAAC TACCATAGAA CAAGGCTTGA TGAACTGAGA 
ATGTTTCTAG AGAATGAGAC CTGGGAGCTT TGTCCTGTGA AGTCAAATTT CAGCATCTTG 
CAACTTCATG AATTTAAATT CATGGAACAG TCTCGTTCCC CATCGGTTTC ACCCAGTAAG 
CAGTCATCTG CAACTTCCTC AAAAACGGTA ACCTTGTTTG AGCAGTACTG CAGTGGTGGG 
AATCCATTTG AAATTCAGGC GGACCACAAA GATGAAGAAA CAGAAGATGT CCTAGCTTCT 
AATGGGTATG AATCTGATGA ACAGGAAAAA AGTGCCTATC AGGAATATGA CAGTGACAGT 
GATGTTCCTG AGGAACTGAA ACGAGATTTC GTTGATGAGC AGACAGGCGA TGCTCCTGTG 
AAAAGTGTTT CTCGAGAGAC CCTAAAAAGC AGGAAGAAAT CAGATTACAG TCTAAATAAA 
GTGAATGCAC CCATCTTAAC AAATACAACA TTGAATGTAA TAAGGCTTGT TGGTAAATAT 
ATGCAGATGA TGAACATTCT TAAGCCAATT GCTTTCGATG TTATCCATTT CATGTCTCAG 
CTCTTTGATT ATTACTTGTA TGCAATATAT ACCTTTTTTG GTCGGAATGA TTCATTGGAA 
TCAACAGGAC TAGGTCTTAG TAGTAGTAGA CTAAGAACAA CTCTAAACAG GATACAAGAA 
AGCCTCATTG ATCTGGAGGT TTCGGCTGAT TCGGCTGCCA CGCTCACAGC AGCAGAAGAG 
AGGAAGGAGA AGGTGCCGAG TCCTCATCTG AGTCACCTAG TGGTTTTGAC CTCTGGGGAC 
ACACTGTATG GGTTGGCGGA AAGAGTGGTC GCCACAGAAT CCTTGGTATT CTTGGCTGAA 
CAGTTTGAGT TCCTTCAGCC TCATCTGGAT GCTGTAATGC CTGCAGTCAA AAAACCCTTT 
CTTCAGCAGT TTTATTCTCA GACGGTCTCA ACGGCCAGTG AACTACGCAA ACCAATTTAC 
TGGATTGTAG CTGGTAAAGC CATTGATTAT GAACAGATGC TGCTGCTGAT GGCTAATGTG 
AAATGGGATG TAAAAGAAAT TATGTCACAG CACAACATAT ATGTAGATAC GCTGTTAAAG 
GAATTTGAGC AGTTTAACAT GCGGTTGAAT GAAGTTTCTA AGAGAGTTCG GATACCCTTG 
CCTGTGTCTA ATATACTTTG GGAACATTGT ATACGCCTCG CTAATAGAAC TATTGTGGAA 
GGATACGCCA ACGTCAAGAA GTGTAGTAAT GAGGGTCGTG CCTTGATGCA ATTGGATTTT 
CAACAGTTTT TAATGAAACT TGAAAAGCTA ACTGATATCA GACCCATTCC GGATAAAGAA 
TTTGTGGAAA CCTATATTAA AGCGTACTAC CTAACTGAGA ATGACATGGA ACGCTGGATC 
AAAGAGCACA GGGAGTATTC AACAAAGCAG CTGACCAACC TGGTAAATGT TTGCCTGGGA 
TCCCATATCA ATAAGAAAGC AAGACAAAAA CTTCTCTCAG CGATAGATGA TATAGACAGA 
CCCAAAAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019815244.1
CDS	97..2868
Translation	MQKIKSLMTRQGLKSPPESFSDLGAIESLRVPGKKLPPVLNLQELEEYRDKLKQQQAAVSKKVADLILEKQPAYVKELERVTSLQTGLQLAAVICTNGRRHLNIAKEGFTQASLGLLANQRKRQLLIGLLKSLRTIKTLQRTDVRLSEMLEEEDYPGAIQLCLECQKAASTFKHYSCISELNSKLQDTLEQIEEQLDVALSKICKNFDVNHYTKVQQAYRLLGKTQTAMDQLHMHFTQAIHNTVFQVVLGYVELCAGNTDTKFQKLQYKDLCTHVTPDSYnullPCLADLCKALWEVMLSYYRTMEWHEKHDNEDTTSASEGSNMMGAEETSFDRGYIKKKLEHGLTRIWQDVQLKVKTYLLGTDLSIFKYDDFIFVLDIISRLMQVGEEFCGSKSEVLQESIRKQSVNYFKNYHRTRLDELRMFLENETWELCPVKSNFSILQLHEFKFMEQSRSPSVSPSKQSSATSSKTVTLFEQYCSGGNPFEIQADHKDEETEDVLASNGYESDEQEKSAYQEYDSDSDVPEELKRDFVDEQTGDAPVKSVSRETLKSRKKSDYSLNKVNAPILTNTTLNVIRLVGKYMQMMNILKPIAFDVIHFMSQLFDYYLYAIYTFFGRNDSLESTGLGLSSSRLRTTLNRIQESLIDLEVSADSAATLTAAEERKEKVPSPHLSHLVVLTSGDTLYGLAERVVATESLVFLAEQFEFLQPHLDAVMPAVKKPFLQQFYSQTVSTASELRKPIYWIVAGKAIDYEQMLLLMANVKWDVKEIMSQHNIYVDTLLKEFEQFNMRLNEVSKRVRIPLPVSNILWEHCIRLANRTIVEGYANVKKCSNEGRALMQLDFQQFLMKLEKLTDIRPIPDKEFVETYIKAYYLTENDMERWIKEHREYSTKQLTNLVNVCLGSHINKKARQKLLSAIDDIDRPKR

Target ORF information:

RefSeq Version	XM_019959685.1
Organism	Bos indicus(Bos taurus indicus)
Definition	Bos indicus VPS50, EARP/GARPII complex subunit (VPS50), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019959685.1

ORF Insert Sequence:

ATGCAAAAAA TCAAATCTCT TATGACCCGG CAGGGTCTGA AAAGTCCTCC AGAGAGCTTC 
AGTGATCTTG GTGCCATTGA GAGTCTCCGG GTCCCTGGAA AGAAGCTCCC TCCTGTTCTC 
AATTTGCAAG AATTAGAGGA GTACAGAGAC AAATTGAAAC AACAGCAAGC TGCAGTCTCA 
AAAAAAGTAG CAGATTTAAT CCTTGAAAAA CAGCCTGCTT ATGTCAAGGA ACTTGAAAGA 
GTTACCTCAT TACAAACAGG TCTTCAGTTA GCTGCTGTAA TCTGCACCAA TGGGAGGAGA 
CACTTGAATA TTGCAAAGGA AGGTTTTACT CAAGCTAGTT TAGGCCTCCT TGCAAATCAG 
AGGAAACGTC AGTTGCTGAT TGGACTTCTG AAATCTCTGA GAACGATAAA AACATTGCAA 
AGAACAGATG TCCGCCTAAG TGAAATGCTG GAGGAGGAAG ACTATCCAGG AGCTATTCAG 
CTGTGCCTGG AATGTCAGAA AGCTGCCAGC ACTTTTAAAC ATTACAGTTG CATAAGTGAA 
CTGAATTCAA AGTTGCAAGA TACTTTAGAA CAAATTGAGG AACAGTTGGA TGTTGCTCTG 
TCCAAAATAT GCAAGAATTT TGACGTTAAC CACTACACCA AAGTTCAACA AGCTTATCGA 
CTTCTTGGAA AAACACAGAC AGCAATGGAT CAACTTCATA TGCACTTCAC CCAAGCCATT 
CATAACACCG TGTTTCAAGT TGTTCTTGGC TATGTGGAAC TATGTGCAGG AAACACAGAT 
ACAAAATTCC AAAAGCTGCA ATATAAGGAT CTCTGTACGC ATGTTACACC AGACAGCTAT 
ATYCCATGCC TTGCTGACCT GTGCAAAGCC CTGTGGGAAG TTATGCTCAG CTATTACAGG 
ACTATGGAAT GGCATGAAAA GCATGATAAT GAGGATACTA CTTCTGCTTC TGAAGGGAGT 
AATATGATGG GTGCTGAAGA GACAAGTTTT GATCGTGGCT ACATAAAAAA GAAATTAGAA 
CATGGACTTA CACGAATATG GCAGGATGTT CAGCTAAAAG TTAAAACCTA CTTGCTTGGA 
ACTGATTTGT CTATATTCAA ATATGATGAT TTCATCTTTG TTTTGGATAT AATCAGCAGG 
TTGATGCAAG TTGGAGAAGA ATTTTGTGGT AGCAAGTCTG AAGTTTTGCA AGAATCTATT 
AGAAAACAAA GTGTCAATTA TTTTAAGAAC TACCATAGAA CAAGGCTTGA TGAACTGAGA 
ATGTTTCTAG AGAATGAGAC CTGGGAGCTT TGTCCTGTGA AGTCAAATTT CAGCATCTTG 
CAACTTCATG AATTTAAATT CATGGAACAG TCTCGTTCCC CATCGGTTTC ACCCAGTAAG 
CAGTCATCTG CAACTTCCTC AAAAACGGTA ACCTTGTTTG AGCAGTACTG CAGTGGTGGG 
AATCCATTTG AAATTCAGGC GGACCACAAA GATGAAGAAA CAGAAGATGT CCTAGCTTCT 
AATGGGTATG AATCTGATGA ACAGGAAAAA AGTGCCTATC AGGAATATGA CAGTGACAGT 
GATGTTCCTG AGGAACTGAA ACGAGATTTC GTTGATGAGC AGACAGGCGA TGCTCCTGTG 
AAAAGTGTTT CTCGAGAGAC CCTAAAAAGC AGGAAGAAAT CAGATTACAG TCTAAATAAA 
GTGAATGCAC CCATCTTAAC AAATACAACA TTGAATGTAA TAAGGCTTGT TGGTAAATAT 
ATGCAGATGA TGAACATTCT TAAGCCAATT GCTTTCGATG TTATCCATTT CATGTCTCAG 
CTCTTTGATT ATTACTTGTA TGCAATATAT ACCTTTTTTG GTCGGAATGA TTCATTGGAA 
TCAACAGGAC TAGGTCTTAG TAGTAGTAGA CTAAGAACAA CTCTAAACAG GATACAAGAA 
AGCCTCATTG ATCTGGAGGT TTCGGCTGAT TCGGCTGCCA CGCTCACAGC AGCAGAAGAG 
AGGAAGGAGA AGGTGCCGAG TCCTCATCTG AGTCACCTAG TGGTTTTGAC CTCTGGGGAC 
ACACTGTATG GGTTGGCGGA AAGAGTGGTC GCCACAGAAT CCTTGGTATT CTTGGCTGAA 
CAGTTTGAGT TCCTTCAGCC TCATCTGGAT GCTGTAATGC CTGCAGTCAA AAAACCCTTT 
CTTCAGCAGT TTTATTCTCA GACGGTCTCA ACGGCCAGTG AACTACGCAA ACCAATTTAC 
TGGATTGTAG CTGGTAAAGC CATTGATTAT GAACAGATGC TGCTGCTGAT GGCTAATGTG 
AAATGGGATG TAAAAGAAAT TATGTCACAG CACAACATAT ATGTAGATAC GCTGTTAAAG 
GAATTTGAGC AGTTTAACAT GCGGTTGAAT GAAGTTTCTA AGAGAGTTCG GATACCCTTG 
CCTGTGTCTA ATATACTTTG GGAACATTGT ATACGCCTCG CTAATAGAAC TATTGTGGAA 
GGATACGCCA ACGTCAAGAA GTGTAGTAAT GAGGGTCGTG CCTTGATGCA ATTGGATTTT 
CAACAGTTTT TAATGAAACT TGAAAAGCTA ACTGATATCA GACCCATTCC GGATAAAGAA 
TTTGTGGAAA CCTATATTAA AGCGTACTAC CTAACTGAGA ATGACATGGA ACGCTGGATC 
AAAGAGCACA GGGAGTATTC AACAAAGCAG CTGACCAACC TGGTAAATGT TTGCCTGGGA 
TCCCATATCA ATAAGAAAGC AAGACAAAAA CTTCTCTCAG CGATAGATGA TATAGACAGA 
CCCAAAAGAT AA