LOC100647253 cDNA ORF clone, Bombus terrestris(buff-tailed bumblebee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100647253
Entrez Gene ID	100647253
Full Name	adenylate kinase 8
Gene Type	protein-coding
Organism	Bombus terrestris(buff-tailed bumblebee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012320890.2	XP_012176280.1	adenylate kinase 8

Pathways from BioSystems
Gene Ontology

The following LOC100647253 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100647253 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBo66594	XM_012320890.2 Latest version!	Bombus terrestris adenylate kinase 8 (LOC100647253), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100647253 ( XM_012320890.2 ) cDNA ORF clone, Bombus terrestris(buff-tailed bumblebee) -> XP_012176280.1 Bombus terrestris adenylate kinase 8 (LOC100647253), mRNA.

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CloneID	OBo66594
Clone ID Related Accession (Same CDS sequence)	XM_012320890.2
Accession Version	XM_012320890.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-24
Organism	Bombus terrestris(buff-tailed bumblebee)
Product	adenylate kinase 8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_015764.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 25, 2017 this sequence version replaced XM_012320890.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombus terrestris Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGAT TGCAGGAAAT GGAGAAGAAA GCGCGTACCA TTAATGCAAG ATTTATGGCT 
TATATGGAGA AACATCGCTT ATACGAACTG TTTTACGAAA TAGCAACTCA ATTAATGATC 
CAAAAACCCG AAGATCATCT TGTATTTATG AAGCAATATC TTCAACTTGC TGCTAAAAGA 
TTCAATGTTC CTAAAATTAT CCTTATCGCA CCTGCAAACT TTGATAGAAT GGCCTTAGCT 
AATGTTCTTC ACGAAGAATT GGGTGTACAT CCTTTAACCT TGAAAGATCT TCGTAAAGCT 
TGCCCTCAAC ATGACGATGA TTGTCAGTGC GAGGAATCGG AAGATCTTGC ATATGCAATG 
AAGAAAATTT TACAACGCGG AACTTTTCAT GAAAGTGGAT GGCTATTAGT TGACTTACCA 
AGAACGAAAA GAGAAGCACA AATATTCCAG CGTACCGGAA TAATACCAAC GCATGTGATA 
CAACTTATAG TTTCAGATGA CAATGAAAAC TGGGGAAACA TTTGCACAGA TGCGTTCGCT 
TGCCGTGAAA CATGTAATCC TACAGACACA GAGAAAAATA TAAAAAATCG TCGTTACTTG 
AAAAATTGTC GGGGCTTATT GGAAGCGTAC AGGCATTGTT TAATAGCAGT TGAGGTAGGT 
ATCAGGTCCA TCGACGAACT TGGGAAAGAT TGTGCAAAAC TGGCAAAAAC AAGGAAACAT 
TGTGGAGCAC CGTCGCTTTT TCGCATCGCG CTTATTGGAT TTAGGGGATC TGGTTGCACT 
ACATTAGCAA AATATTTGGC CGAACGATTC AATCTTGTTC ATATTGATTA CGATTATATC 
GCGGAACAGG CACGCTTACA ACAGAATCCT TTGGGAGAAA TGCTTCGATT GTTCGAGTAC 
AAATGGGGAG AAAGACCCAA GCCTGAAATT AGGATTCAAA TCGTCGAAAA ACACATATCC 
GGGTATGAAT GTTTGAAAAG GGGTTGGGTT TTAACTGGAT ATCCAAAAAC TGTAGAAGAT 
TTTAAACTAT TAGATTTGAA TCCTACGCCT CCAAATAGAG TAATATTTGT GGAGGCGAGT 
GGTGATATTT GCAGAGAAAG ACTGCTTAAT CGTAGATACA ATATTGTCAC CGGAAGCAAG 
CACATTTTGT CGACAGCAAG TTATGCCGTT AACGACGATT GCAAATTAGG TGTACATTCC 
AGAGATTTCA GACTAATCGT AGAACGAGAT CTTCAAGAGT ACGAAGAGAA TGTGATCGAC 
ATGATGCAGT ATGCAGGTGA ATCCGCGATA AAAATCGATG GAAACGAGGA AGAAAGGATC 
GTCCGAGAAA AGGTAGAAGC AAGTATAATG CGTCCAGTTC CACCCTGGTA TTCGAGAGTA 
CCAAGACCTT CACCTGCGAT CGATCCCATG AGCAACGAAT TTGACCCGGA TGACGAGCCC 
GACTCCAGCG TGTTTGATGA CATACGTGCC CCCGAGCCGA CATATACTTT TATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012176280.1
CDS	63..1559
Translation	MAGLQEMEKKARTINARFMAYMEKHRLYELFYEIATQLMIQKPEDHLVFMKQYLQLAAKRFNVPKIILIAPANFDRMALANVLHEELGVHPLTLKDLRKACPQHDDDCQCEESEDLAYAMKKILQRGTFHESGWLLVDLPRTKREAQIFQRTGIIPTHVIQLIVSDDNENWGNICTDAFACRETCNPTDTEKNIKNRRYLKNCRGLLEAYRHCLIAVEVGIRSIDELGKDCAKLAKTRKHCGAPSLFRIALIGFRGSGCTTLAKYLAERFNLVHIDYDYIAEQARLQQNPLGEMLRLFEYKWGERPKPEIRIQIVEKHISGYECLKRGWVLTGYPKTVEDFKLLDLNPTPPNRVIFVEASGDICRERLLNRRYNIVTGSKHILSTASYAVNDDCKLGVHSRDFRLIVERDLQEYEENVIDMMQYAGESAIKIDGNEEERIVREKVEASIMRPVPPWYSRVPRPSPAIDPMSNEFDPDDEPDSSVFDDIRAPEPTYTFI

Target ORF information:

RefSeq Version	XM_012320890.2
Organism	Bombus terrestris(buff-tailed bumblebee)
Definition	Bombus terrestris adenylate kinase 8 (LOC100647253), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012320890.2

ORF Insert Sequence:

ATGGCGGGAT TGCAGGAAAT GGAGAAGAAA GCGCGTACCA TTAATGCAAG ATTTATGGCT 
TATATGGAGA AACATCGCTT ATACGAACTG TTTTACGAAA TAGCAACTCA ATTAATGATC 
CAAAAACCCG AAGATCATCT TGTATTTATG AAGCAATATC TTCAACTTGC TGCTAAAAGA 
TTCAATGTTC CTAAAATTAT CCTTATCGCA CCTGCAAACT TTGATAGAAT GGCCTTAGCT 
AATGTTCTTC ACGAAGAATT GGGTGTACAT CCTTTAACCT TGAAAGATCT TCGTAAAGCT 
TGCCCTCAAC ATGACGATGA TTGTCAGTGC GAGGAATCGG AAGATCTTGC ATATGCAATG 
AAGAAAATTT TACAACGCGG AACTTTTCAT GAAAGTGGAT GGCTATTAGT TGACTTACCA 
AGAACGAAAA GAGAAGCACA AATATTCCAG CGTACCGGAA TAATACCAAC GCATGTGATA 
CAACTTATAG TTTCAGATGA CAATGAAAAC TGGGGAAACA TTTGCACAGA TGCGTTCGCT 
TGCCGTGAAA CATGTAATCC TACAGACACA GAGAAAAATA TAAAAAATCG TCGTTACTTG 
AAAAATTGTC GGGGCTTATT GGAAGCGTAC AGGCATTGTT TAATAGCAGT TGAGGTAGGT 
ATCAGGTCCA TCGACGAACT TGGGAAAGAT TGTGCAAAAC TGGCAAAAAC AAGGAAACAT 
TGTGGAGCAC CGTCGCTTTT TCGCATCGCG CTTATTGGAT TTAGGGGATC TGGTTGCACT 
ACATTAGCAA AATATTTGGC CGAACGATTC AATCTTGTTC ATATTGATTA CGATTATATC 
GCGGAACAGG CACGCTTACA ACAGAATCCT TTGGGAGAAA TGCTTCGATT GTTCGAGTAC 
AAATGGGGAG AAAGACCCAA GCCTGAAATT AGGATTCAAA TCGTCGAAAA ACACATATCC 
GGGTATGAAT GTTTGAAAAG GGGTTGGGTT TTAACTGGAT ATCCAAAAAC TGTAGAAGAT 
TTTAAACTAT TAGATTTGAA TCCTACGCCT CCAAATAGAG TAATATTTGT GGAGGCGAGT 
GGTGATATTT GCAGAGAAAG ACTGCTTAAT CGTAGATACA ATATTGTCAC CGGAAGCAAG 
CACATTTTGT CGACAGCAAG TTATGCCGTT AACGACGATT GCAAATTAGG TGTACATTCC 
AGAGATTTCA GACTAATCGT AGAACGAGAT CTTCAAGAGT ACGAAGAGAA TGTGATCGAC 
ATGATGCAGT ATGCAGGTGA ATCCGCGATA AAAATCGATG GAAACGAGGA AGAAAGGATC 
GTCCGAGAAA AGGTAGAAGC AAGTATAATG CGTCCAGTTC CACCCTGGTA TTCGAGAGTA 
CCAAGACCTT CACCTGCGAT CGATCCCATG AGCAACGAAT TTGACCCGGA TGACGAGCCC 
GACTCCAGCG TGTTTGATGA CATACGTGCC CCCGAGCCGA CATATACTTT TATCTAA