LOC100747183 cDNA ORF clone, Bombus impatiens(common eastern bumble bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100747183
Entrez Gene ID	100747183
Full Name	elongation factor Tu, mitochondrial-like
Gene Type	protein-coding
Organism	Bombus impatiens(common eastern bumble bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003494197.2	XP_003494245.1	elongation factor Tu, mitochondrial-like

Pathways from BioSystems
Gene Ontology

The following LOC100747183 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100747183 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBo111157	XM_003494197.2 Latest version!	Bombus impatiens elongation factor Tu, mitochondrial-like (LOC100747183), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100747183 ( XM_003494197.2 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_003494245.1 Bombus impatiens elongation factor Tu, mitochondrial-like (LOC100747183), mRNA.

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CloneID	OBo111157
Clone ID Related Accession (Same CDS sequence)	XM_003494197.2
Accession Version	XM_003494197.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-27
Organism	Bombus impatiens(common eastern bumble bee)
Product	elongation factor Tu, mitochondrial-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_178210.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2015 this sequence version replaced XM_003494197.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombus impatiens Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAAG AAAAATTTGA ACGTACAAAA CCCCACGTTA ACGTCGGTAC AATCGGCCAC 
GTTGACCATG GTAAAACAAC TTTAACTGCT GCGATCACCA GCGTACTTGC TAAAAAATTC 
GGTGGTCAAG CACGAGCATT CGATCAAATC GATAATGCAC CAGAAGAAAA AGCACGTGGT 
ATCACCATCA ATACATCACA CGTTGAGTAC GATACGCCAA CTCGTCACTA CGCACACGTA 
GACTGCCCGG GCCATGCTGA CTATGTTAAA AACATGATCA CAGGTGCGGC ACAAATGGAC 
GGAGCTATCT TAGTAGTAGC AGCAACAGAT GGTCCTATGC CACAAACACG TGAGCACATC 
CTTTTAGGTC GTCAAGTAGG TGTTCCTTAC ATCATCGTAT TCTTAAACAA ATGTGATATG 
GTTGATGACG AAGAGTTATT AGAATTAGTT GAAATGGAAG TACGTGAACT TCTATCTCAA 
TATGATTTCC CAGGTGATGA TACACCAGTG ATTCGTGGTT CAGCATTACA AGCGTTAAAC 
GGTGTTGCTG AGTGGGAAGA CAAAATTGTT GAATTAGCGG AAGCGTTAGA CACTTATATC 
CCAGAGCCAG AGCGTGACAT TGATAAACCA TTCTTATTAC CAATCGAAGA TGTATTCTCA 
ATCTCAGGAC GTGGTACAGT AGTAACAGGT CGTGTAGAGC GTGGTATCAT CAAAGTTGGT 
GATGAAGTTG AAATCGTAGG TATCAAAGAT ACAGCGAAAA CAACTTGTAC TGGTGTTGAG 
ATGTTCCGTA AACTGTTAGA CGAAGGTCGT GCAGGTGAGA ACGTAGGTGT GTTATTACGT 
GGTACTAAAC GTGAAGAAAT CGAACGTGGT CAAGTATTAG CGAAACCAGG TTCAATTAAA 
CCACATACTG ATTTCGAATC AGAAGTGTAT ATCTTAAGCA AAGATGAAGG TGGTCGTCAT 
ACTCCATTCT TCAAAGGTTA CCGTCCACAG TTCTACTTCC GTACAACTGA CGTAACAGGA 
ACTATCGAGT TACCAGAAGG CGTAGAGATG GTAATGCCAG GTGATAACAT TAAGATGACA 
GTATCATTAA TTCACCCAAT CGCGATGGAT GAAGGTTTAC GTTTCGCTAT CCGTGAAGGT 
GGTCGTACTG TTGGTGCTGG TGTTGTTGCT AAAATTATTA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003494245.1
CDS	16..1200
Translation	MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITSVLAKKFGGQARAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPVIRGSALQALNGVAEWEDKIVELAEALDTYIPEPERDIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGDEVEIVGIKDTAKTTCTGVEMFRKLLDEGRAGENVGVLLRGTKREEIERGQVLAKPGSIKPHTDFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMTVSLIHPIAMDEGLRFAIREGGRTVGAGVVAKIIK

Target ORF information:

RefSeq Version	XM_003494197.2
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens elongation factor Tu, mitochondrial-like (LOC100747183), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003494197.2

ORF Insert Sequence:

ATGTCTAAAG AAAAATTTGA ACGTACAAAA CCCCACGTTA ACGTCGGTAC AATCGGCCAC 
GTTGACCATG GTAAAACAAC TTTAACTGCT GCGATCACCA GCGTACTTGC TAAAAAATTC 
GGTGGTCAAG CACGAGCATT CGATCAAATC GATAATGCAC CAGAAGAAAA AGCACGTGGT 
ATCACCATCA ATACATCACA CGTTGAGTAC GATACGCCAA CTCGTCACTA CGCACACGTA 
GACTGCCCGG GCCATGCTGA CTATGTTAAA AACATGATCA CAGGTGCGGC ACAAATGGAC 
GGAGCTATCT TAGTAGTAGC AGCAACAGAT GGTCCTATGC CACAAACACG TGAGCACATC 
CTTTTAGGTC GTCAAGTAGG TGTTCCTTAC ATCATCGTAT TCTTAAACAA ATGTGATATG 
GTTGATGACG AAGAGTTATT AGAATTAGTT GAAATGGAAG TACGTGAACT TCTATCTCAA 
TATGATTTCC CAGGTGATGA TACACCAGTG ATTCGTGGTT CAGCATTACA AGCGTTAAAC 
GGTGTTGCTG AGTGGGAAGA CAAAATTGTT GAATTAGCGG AAGCGTTAGA CACTTATATC 
CCAGAGCCAG AGCGTGACAT TGATAAACCA TTCTTATTAC CAATCGAAGA TGTATTCTCA 
ATCTCAGGAC GTGGTACAGT AGTAACAGGT CGTGTAGAGC GTGGTATCAT CAAAGTTGGT 
GATGAAGTTG AAATCGTAGG TATCAAAGAT ACAGCGAAAA CAACTTGTAC TGGTGTTGAG 
ATGTTCCGTA AACTGTTAGA CGAAGGTCGT GCAGGTGAGA ACGTAGGTGT GTTATTACGT 
GGTACTAAAC GTGAAGAAAT CGAACGTGGT CAAGTATTAG CGAAACCAGG TTCAATTAAA 
CCACATACTG ATTTCGAATC AGAAGTGTAT ATCTTAAGCA AAGATGAAGG TGGTCGTCAT 
ACTCCATTCT TCAAAGGTTA CCGTCCACAG TTCTACTTCC GTACAACTGA CGTAACAGGA 
ACTATCGAGT TACCAGAAGG CGTAGAGATG GTAATGCCAG GTGATAACAT TAAGATGACA 
GTATCATTAA TTCACCCAAT CGCGATGGAT GAAGGTTTAC GTTTCGCTAT CCGTGAAGGT 
GGTCGTACTG TTGGTGCTGG TGTTGTTGCT AAAATTATTA AATAA