LOC100745898 cDNA ORF clone, Bombus impatiens(common eastern bumble bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100745898
Entrez Gene ID	100745898
Full Name	uncharacterized LOC100745898
Gene Type	protein-coding
Organism	Bombus impatiens(common eastern bumble bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012385926.1	XP_012241349.1	uncharacterized protein LOC100745898 isoform X2
XM_024367821.1	XP_024223589.1	uncharacterized protein LOC100745898 isoform X2
XM_012385926.2	XP_012241349.1	uncharacterized protein LOC100745898 isoform X2
XM_012385927.1	XP_012241350.1	uncharacterized protein LOC100745898 isoform X3
XM_003489654.3	XP_003489702.1	uncharacterized protein LOC100745898 isoform X1
XM_003489654.2	XP_003489702.1	uncharacterized protein LOC100745898 isoform X1
XM_024367822.1	XP_024223590.1	uncharacterized protein LOC100745898 isoform X2
XM_012385927.2	XP_012241350.1	uncharacterized protein LOC100745898 isoform X3

Pathways from BioSystems
Gene Ontology

The following LOC100745898 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100745898 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBo104096	XM_024367821.1 Latest version!	Bombus impatiens uncharacterized LOC100745898 (LOC100745898), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBo104096	XM_012385926.2 Latest version!	Bombus impatiens uncharacterized LOC100745898 (LOC100745898), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBo104096	XM_024367822.1 Latest version!	Bombus impatiens uncharacterized LOC100745898 (LOC100745898), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBo104097	XM_012385927.2 Latest version!	Bombus impatiens uncharacterized LOC100745898 (LOC100745898), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBo104095	XM_003489654.3 Latest version!	Bombus impatiens uncharacterized LOC100745898 (LOC100745898), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
View Old Accession Versions >>
OBo104096	XM_012385926.1	Bombus impatiens uncharacterized LOC100745898 (LOC100745898), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBo104095	XM_003489654.2	Bombus impatiens uncharacterized LOC100745898 (LOC100745898), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBo104097	XM_012385927.1	Bombus impatiens uncharacterized LOC100745898 (LOC100745898), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100745898 ( XM_012385926.1 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_012241349.1 Bombus impatiens uncharacterized LOC100745898 (LOC100745898), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBo104096
Clone ID Related Accession (Same CDS sequence)	XM_012385926.1 , XM_024367821.1 , XM_012385926.2 , XM_024367822.1
Accession Version	XM_012385926.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-27
Organism	Bombus impatiens(common eastern bumble bee)
Product	uncharacterized protein LOC100745898 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176780.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombus impatiens Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAACTC CTCCAACATC ATTTTTTAGT CCTGAATGCC CCTTGTCTAA TACAACCAAT 
CTACGGGGTC AAACTACCCA AAGATCTAAA TCGCGGGTGG AGGCAATGTT GGAGCGTGCT 
CTGATACAGC CACAACAGTG TTCCGTGGAT CCACTCTACA ACGCTCAAAA TTGGGGAATT 
CCTATTTGGG CAATTGATAA ACTTCAGTCT TGGCTTGACA AAATCTACTC ATCTGTTAAA 
GACACGAATC ATTTGAAACA ATTAAGACAC TCACATCAAC AGAGTTCAAC TAAAGATATA 
AAGGTCAGAC ACCTCAAGGG TCCTTATTTG AAATTTGAAT CCTTTCAGAG GAATACTAGA 
CCTGTATTTG TTGAACTACC TGTGTGGCCA ACATTGAATT TTCACGGTGA TCCAGGCTCT 
TGTCCTTTCA ATACGAAGAG ACGAGAAAAA CTGGAAAAAT TAGGGAAAGA AGTAAAAGAA 
AACAGGGAAG GTATTCCGGC TATCAACCAA GAGGAGAAAA AAGAAATGAC TCGGCGACCA 
CGAGCAACTG TCCGCACTCG AAGAACAGAA CAATTAGCTT CCGGTTATTG TGAAATTTGC 
CGTATTAGAT ATAGAGATCT GGCCAAACAT GTGCAAAGCG ATCAGCATCT TAGCTTTATT 
CAAAATGATG ATAATTTCTT GTCTTTGGAT AAATTGATCA ATGCAGGAGC CAATGTAGAA 
GCGTTTTTAA AACTAAACAG AGGAAAAAAT ATAGAAAAAG ATTGCAATTT GTTTTCCAAT 
GGGGATCGTA ATCTTCATGA CACAGTATTG CCAGAAGGGA AGGTTAATAG AAATGCAAAA 
AGTTTAGGGG ATTTTGACGT TGGAGACATA AAGATGGTAC AACGTAATGG CGCACGTCGG 
AATCTCAGTT TAAGATTAAA CTCCTCTCAT AATTTACGTA CACGTACAAG ACACGAAAGC 
GGCCATTTAT TAAGATCAAA AGGTAGTCCC TGGCATGAAG CCGATAAAAC GGAGAAGTTC 
TACGACGAAT TCGAAGGTTT TACTATCAAA AAGCGAGCGA AAGGGACAAT TTGGATCGAA 
GAAGATGCAC CAGGAGACAA ATATATAGAA GAACATGAAT TAAAAGAATC TAAGCAAAAT 
GAATACAAAA TTAAAACATT TTCGGCGGAA TTAGAAGAAA AATGTTGTAA TGAGAAAAAT 
TGTGAAACAT ATAACAAACT TAAAGATACT AACAATCAGG ACATACAAAG GACAATAAAT 
TGTAATAACG AAGAAAATAG TATTGGAAAT GACCCTGAAC GCATCAAACA AGAACAAACT 
CATCCAAAGA GTAAAAATCA TGATCAAATT AATGGAAACA TAAAAAATGG TTGTAATGAA 
AATCTTTTAA GTACAAATGA TAACAGTCGT ATTAAACCTT GTAAAGTTTC GCCTGGTAAC 
GAAACTTTAA AAGAATTGAC TTGCAAATTA CAGTTAGAAA ATACTCCATC AAGTGCAAAA 
AGGTTATCAG AGCACAATGA TATCAATACA GGCACAATCT GTAATGGTCA TTCTGTAAGG 
GATGAACAGA AAATTAATAG AACACTGAAT AATACAGAAA AGAACGAAGT AGCAAAAGAA 
GAGAAATCAA AATGTTGCAA GTCAAATAGA AGAGGTAGTC GGAGCGTCAG AGGACGGCAC 
AGGTTGTCCG TTGAAGAACG TTTGATTGAA GATAATCGTG CATATTATAA AGTGGAAGTG 
TTAGGAAGTA AGTTAAGATC AAGTGTATTG TCAAACAATA ATTCTCAATG TACAGTTCAA 
AAGGATGGGG ATAGTGAAGA AAAGAAGGAA GTGCCATCTA GTGAGAAACC AGTAGTTGTA 
CGATTTAAAC GTGTAAGAAA GTCGGAACTA TCATTACTAA GCGATGAAGC GGAGTCTTTT 
ATGTTTAGGG AACTTAAACG AGATGATTCA AGTGAAATAA GCGATGACGA ACAGAGTAGC 
GTATTACCAA GGGATACTGA AAGTGAGTGC AACGATAGCG GCAATTCTAT CTGTCCTAGT 
TCATTCTTAA CTTCTAGTTC ACCTGCAAAA TCGGAAACTA CCGAAGACGA TTCTCAAGAC 
TCTTTAAGTT TGGGACGAGC TAGAAAAAAA AGACGGACAC AAGCAGAAGC GCTGATCAAA 
GATAATGTTG ATTATTACAA ATTCGAAACT CCTGGGAGCA GATTAAGGTA TCAGGCGCCT 
CTAACTGGTA TTAAAGAAAA TCAGGAAGAA ATAGAAGGTA GTATTGTCGA GAAAGGTATA 
GAAATAGCAA AGGTGTATCC GTCCAAACCC TCACCAGAAG TTGAAAAAAT GCAATTTTCT 
TTTGAAGCCG TACCAAAGTC TGAACCATGG TATCAAACGT ATCAGCGGCA AGATGAAGGA 
GCAGAATTTT GGCACTATTT TAGCGAAGGG GACTCACAAA AGCCATTTCT TTTACCATAT 
GAAATTGAAA ATTTTCACGA AATTTTGATA AAAAACCAAA ATAGAGCGGA AAATAAAAGA 
AAAGGTCGCG GACGGAGTAT AGGTTGTATT GGACGATCTC CTAGGAAAAG TCCTCGTTGT 
CATGCTTCCA CATTAGCTAT TATGTCAACA ATTATAAGAA AAAGAGAACA ACAGCAACAA 
CAATCTTCGA ATTTGTACAC AATAGATGAA TGCCAATCCA TCAGGTCACA TGCAAATACT 
CCCAGACCTG ATCAGAAAAC TGAGTTGAAA TCTGATGTGG ATGAAGATCT AAAAGAAATG 
GTAAAAAGTA TCGACGAGAT GCTTAATAAC GCAAACGATA GTTTCGAGTT GATTGAATCG 
TTCGAGCCTG ATACAACACA GATGGAATTG AATTTTTATG AACCAAATAT CCCAAGAGGA 
CCACCTCCAA ACTTGCTTGA ATTATTGGAC AGTTGTCACG ATATCGTAAA CTGTTTAGAA 
AATTCATCAT GTGCAAGTTC CGAGTGTGGT GAGGGAAGTG TCGAGTCACC TTTAAAACGA 
AGGAAGAAGA GGAAAAATAG AACCGGATGG CCTGGAATTA AAATGAAGAG AAGACTCCAA 
AGCAAACCTC TGGCAGACAT AAACTGTGAC AGGGAAAACC TAGTGGAGAA AAATATAGGG 
CAAAGCGAGA AGGATTGTAA TGTGCAGGTC ACGAATATGT CTAACAGATT AACGGAAGAA 
GGTAATATTA AAATAGCTAC ATCCGTTACG AGTTCCATCA CACAAGATGA ACAGTCATTA 
GGCTTCGGCC AGAGGAAGGA CGACGGACGT TTATTCGAAG TGTATACCTC GAATATTTCT 
TCTAACACGC GTCACGACGA AAACGAGAAG AAAGACGTAA GTATAGCGGT TCCTGATAAT 
TCCATGTTAC ATACAACAAA TGCTGAATCC TGCACAGGTT CACTTACATC TGATATATGC 
AACGAGACTG ACAGGAATTT CATATTGAAC AAAGACTATG GATTCAGAAA AAATTTGTCG 
AAGATCGAAG AAATGTCAGA AAATGATCAC GGAAACGATG AAAATCACGA GAACGTGTTT 
CGGAAAGATG TTCATTCTAT ATCGGAACGT CGTTTTATCT CGAACGCAAC CATCAAGAAA 
CGACAACATG ACAACACATC TTCTGAAACT TGCGAATCTC GTGTAGAACT CGAAAATCAT 
GAGATATTGT GTAGAAAGGA CGCCGAGACC AAAATTGTTT CCAGGAAACA TCATAATACT 
AACGGATTAC CGAGAAAGAG GCAACAGAAG ACTCATTCTG AAAACTCTGA CTCTGAAATT 
GAACAGCATC ATCGCCTCCA TCATCATCAC CATCACCACC TTCACCATAG TAATGTATAT 
AAAAGGAACA GAGTAACTTC GCGAAAACGA ATTTGCGCGA AGAAACGGAC GAGAAAAACT 
GACATATCTT CAGATACAGT GTTCGAAGAT GAGAATTGTA AAAAGGATTC CTATTTAAGT 
ATTACATGTA GAAAGCAACA AACTATAAAA GGTACAAAAC GACGAGCCGA TACCATGTCA 
TCGGAAACAC AAAATTCTGA AGTCGATTGT GCATTATCGG GACATGTCAG TCCTACGATT 
ATAACTACTT CAGAACTTGA ACAAAGACGT TCGAGTATTG AGTTTCAACC AGTTGTTAGA 
ATGATGAAGA TTGACGATCA AGTTGAAATG GACCACAGTA TTCTCTCGGT AACGATTGCA 
AGCAACAGAC GGTTAAGAAG TTCCAGTTCT CCGAGATCGA ATACTCAACC GCCGAAAAAA 
CGTTTAAAGA CCAATCGTGG TCAATTCGGA AGGTGGTTAA AAAGTAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012241349.1
CDS	1049..5359
Translation	MPTPPTSFFSPECPLSNTTNLRGQTTQRSKSRVEAMLERALIQPQQCSVDPLYNAQNWGIPIWAIDKLQSWLDKIYSSVKDTNHLKQLRHSHQQSSTKDIKVRHLKGPYLKFESFQRNTRPVFVELPVWPTLNFHGDPGSCPFNTKRREKLEKLGKEVKENREGIPAINQEEKKEMTRRPRATVRTRRTEQLASGYCEICRIRYRDLAKHVQSDQHLSFIQNDDNFLSLDKLINAGANVEAFLKLNRGKNIEKDCNLFSNGDRNLHDTVLPEGKVNRNAKSLGDFDVGDIKMVQRNGARRNLSLRLNSSHNLRTRTRHESGHLLRSKGSPWHEADKTEKFYDEFEGFTIKKRAKGTIWIEEDAPGDKYIEEHELKESKQNEYKIKTFSAELEEKCCNEKNCETYNKLKDTNNQDIQRTINCNNEENSIGNDPERIKQEQTHPKSKNHDQINGNIKNGCNENLLSTNDNSRIKPCKVSPGNETLKELTCKLQLENTPSSAKRLSEHNDINTGTICNGHSVRDEQKINRTLNNTEKNEVAKEEKSKCCKSNRRGSRSVRGRHRLSVEERLIEDNRAYYKVEVLGSKLRSSVLSNNNSQCTVQKDGDSEEKKEVPSSEKPVVVRFKRVRKSELSLLSDEAESFMFRELKRDDSSEISDDEQSSVLPRDTESECNDSGNSICPSSFLTSSSPAKSETTEDDSQDSLSLGRARKKRRTQAEALIKDNVDYYKFETPGSRLRYQAPLTGIKENQEEIEGSIVEKGIEIAKVYPSKPSPEVEKMQFSFEAVPKSEPWYQTYQRQDEGAEFWHYFSEGDSQKPFLLPYEIENFHEILIKNQNRAENKRKGRGRSIGCIGRSPRKSPRCHASTLAIMSTIIRKREQQQQQSSNLYTIDECQSIRSHANTPRPDQKTELKSDVDEDLKEMVKSIDEMLNNANDSFELIESFEPDTTQMELNFYEPNIPRGPPPNLLELLDSCHDIVNCLENSSCASSECGEGSVESPLKRRKKRKNRTGWPGIKMKRRLQSKPLADINCDRENLVEKNIGQSEKDCNVQVTNMSNRLTEEGNIKIATSVTSSITQDEQSLGFGQRKDDGRLFEVYTSNISSNTRHDENEKKDVSIAVPDNSMLHTTNAESCTGSLTSDICNETDRNFILNKDYGFRKNLSKIEEMSENDHGNDENHENVFRKDVHSISERRFISNATIKKRQHDNTSSETCESRVELENHEILCRKDAETKIVSRKHHNTNGLPRKRQQKTHSENSDSEIEQHHRLHHHHHHHLHHSNVYKRNRVTSRKRICAKKRTRKTDISSDTVFEDENCKKDSYLSITCRKQQTIKGTKRRADTMSSETQNSEVDCALSGHVSPTIITTSELEQRRSSIEFQPVVRMMKIDDQVEMDHSILSVTIASNRRLRSSSSPRSNTQPPKKRLKTNRGQFGRWLKSS

Target ORF information:

RefSeq Version	XM_012385926.1
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens uncharacterized LOC100745898 (LOC100745898), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012385926.1

ORF Insert Sequence:

ATGCCAACTC CTCCAACATC ATTTTTTAGT CCTGAATGCC CCTTGTCTAA TACAACCAAT 
CTACGGGGTC AAACTACCCA AAGATCTAAA TCGCGGGTGG AGGCAATGTT GGAGCGTGCT 
CTGATACAGC CACAACAGTG TTCCGTGGAT CCACTCTACA ACGCTCAAAA TTGGGGAATT 
CCTATTTGGG CAATTGATAA ACTTCAGTCT TGGCTTGACA AAATCTACTC ATCTGTTAAA 
GACACGAATC ATTTGAAACA ATTAAGACAC TCACATCAAC AGAGTTCAAC TAAAGATATA 
AAGGTCAGAC ACCTCAAGGG TCCTTATTTG AAATTTGAAT CCTTTCAGAG GAATACTAGA 
CCTGTATTTG TTGAACTACC TGTGTGGCCA ACATTGAATT TTCACGGTGA TCCAGGCTCT 
TGTCCTTTCA ATACGAAGAG ACGAGAAAAA CTGGAAAAAT TAGGGAAAGA AGTAAAAGAA 
AACAGGGAAG GTATTCCGGC TATCAACCAA GAGGAGAAAA AAGAAATGAC TCGGCGACCA 
CGAGCAACTG TCCGCACTCG AAGAACAGAA CAATTAGCTT CCGGTTATTG TGAAATTTGC 
CGTATTAGAT ATAGAGATCT GGCCAAACAT GTGCAAAGCG ATCAGCATCT TAGCTTTATT 
CAAAATGATG ATAATTTCTT GTCTTTGGAT AAATTGATCA ATGCAGGAGC CAATGTAGAA 
GCGTTTTTAA AACTAAACAG AGGAAAAAAT ATAGAAAAAG ATTGCAATTT GTTTTCCAAT 
GGGGATCGTA ATCTTCATGA CACAGTATTG CCAGAAGGGA AGGTTAATAG AAATGCAAAA 
AGTTTAGGGG ATTTTGACGT TGGAGACATA AAGATGGTAC AACGTAATGG CGCACGTCGG 
AATCTCAGTT TAAGATTAAA CTCCTCTCAT AATTTACGTA CACGTACAAG ACACGAAAGC 
GGCCATTTAT TAAGATCAAA AGGTAGTCCC TGGCATGAAG CCGATAAAAC GGAGAAGTTC 
TACGACGAAT TCGAAGGTTT TACTATCAAA AAGCGAGCGA AAGGGACAAT TTGGATCGAA 
GAAGATGCAC CAGGAGACAA ATATATAGAA GAACATGAAT TAAAAGAATC TAAGCAAAAT 
GAATACAAAA TTAAAACATT TTCGGCGGAA TTAGAAGAAA AATGTTGTAA TGAGAAAAAT 
TGTGAAACAT ATAACAAACT TAAAGATACT AACAATCAGG ACATACAAAG GACAATAAAT 
TGTAATAACG AAGAAAATAG TATTGGAAAT GACCCTGAAC GCATCAAACA AGAACAAACT 
CATCCAAAGA GTAAAAATCA TGATCAAATT AATGGAAACA TAAAAAATGG TTGTAATGAA 
AATCTTTTAA GTACAAATGA TAACAGTCGT ATTAAACCTT GTAAAGTTTC GCCTGGTAAC 
GAAACTTTAA AAGAATTGAC TTGCAAATTA CAGTTAGAAA ATACTCCATC AAGTGCAAAA 
AGGTTATCAG AGCACAATGA TATCAATACA GGCACAATCT GTAATGGTCA TTCTGTAAGG 
GATGAACAGA AAATTAATAG AACACTGAAT AATACAGAAA AGAACGAAGT AGCAAAAGAA 
GAGAAATCAA AATGTTGCAA GTCAAATAGA AGAGGTAGTC GGAGCGTCAG AGGACGGCAC 
AGGTTGTCCG TTGAAGAACG TTTGATTGAA GATAATCGTG CATATTATAA AGTGGAAGTG 
TTAGGAAGTA AGTTAAGATC AAGTGTATTG TCAAACAATA ATTCTCAATG TACAGTTCAA 
AAGGATGGGG ATAGTGAAGA AAAGAAGGAA GTGCCATCTA GTGAGAAACC AGTAGTTGTA 
CGATTTAAAC GTGTAAGAAA GTCGGAACTA TCATTACTAA GCGATGAAGC GGAGTCTTTT 
ATGTTTAGGG AACTTAAACG AGATGATTCA AGTGAAATAA GCGATGACGA ACAGAGTAGC 
GTATTACCAA GGGATACTGA AAGTGAGTGC AACGATAGCG GCAATTCTAT CTGTCCTAGT 
TCATTCTTAA CTTCTAGTTC ACCTGCAAAA TCGGAAACTA CCGAAGACGA TTCTCAAGAC 
TCTTTAAGTT TGGGACGAGC TAGAAAAAAA AGACGGACAC AAGCAGAAGC GCTGATCAAA 
GATAATGTTG ATTATTACAA ATTCGAAACT CCTGGGAGCA GATTAAGGTA TCAGGCGCCT 
CTAACTGGTA TTAAAGAAAA TCAGGAAGAA ATAGAAGGTA GTATTGTCGA GAAAGGTATA 
GAAATAGCAA AGGTGTATCC GTCCAAACCC TCACCAGAAG TTGAAAAAAT GCAATTTTCT 
TTTGAAGCCG TACCAAAGTC TGAACCATGG TATCAAACGT ATCAGCGGCA AGATGAAGGA 
GCAGAATTTT GGCACTATTT TAGCGAAGGG GACTCACAAA AGCCATTTCT TTTACCATAT 
GAAATTGAAA ATTTTCACGA AATTTTGATA AAAAACCAAA ATAGAGCGGA AAATAAAAGA 
AAAGGTCGCG GACGGAGTAT AGGTTGTATT GGACGATCTC CTAGGAAAAG TCCTCGTTGT 
CATGCTTCCA CATTAGCTAT TATGTCAACA ATTATAAGAA AAAGAGAACA ACAGCAACAA 
CAATCTTCGA ATTTGTACAC AATAGATGAA TGCCAATCCA TCAGGTCACA TGCAAATACT 
CCCAGACCTG ATCAGAAAAC TGAGTTGAAA TCTGATGTGG ATGAAGATCT AAAAGAAATG 
GTAAAAAGTA TCGACGAGAT GCTTAATAAC GCAAACGATA GTTTCGAGTT GATTGAATCG 
TTCGAGCCTG ATACAACACA GATGGAATTG AATTTTTATG AACCAAATAT CCCAAGAGGA 
CCACCTCCAA ACTTGCTTGA ATTATTGGAC AGTTGTCACG ATATCGTAAA CTGTTTAGAA 
AATTCATCAT GTGCAAGTTC CGAGTGTGGT GAGGGAAGTG TCGAGTCACC TTTAAAACGA 
AGGAAGAAGA GGAAAAATAG AACCGGATGG CCTGGAATTA AAATGAAGAG AAGACTCCAA 
AGCAAACCTC TGGCAGACAT AAACTGTGAC AGGGAAAACC TAGTGGAGAA AAATATAGGG 
CAAAGCGAGA AGGATTGTAA TGTGCAGGTC ACGAATATGT CTAACAGATT AACGGAAGAA 
GGTAATATTA AAATAGCTAC ATCCGTTACG AGTTCCATCA CACAAGATGA ACAGTCATTA 
GGCTTCGGCC AGAGGAAGGA CGACGGACGT TTATTCGAAG TGTATACCTC GAATATTTCT 
TCTAACACGC GTCACGACGA AAACGAGAAG AAAGACGTAA GTATAGCGGT TCCTGATAAT 
TCCATGTTAC ATACAACAAA TGCTGAATCC TGCACAGGTT CACTTACATC TGATATATGC 
AACGAGACTG ACAGGAATTT CATATTGAAC AAAGACTATG GATTCAGAAA AAATTTGTCG 
AAGATCGAAG AAATGTCAGA AAATGATCAC GGAAACGATG AAAATCACGA GAACGTGTTT 
CGGAAAGATG TTCATTCTAT ATCGGAACGT CGTTTTATCT CGAACGCAAC CATCAAGAAA 
CGACAACATG ACAACACATC TTCTGAAACT TGCGAATCTC GTGTAGAACT CGAAAATCAT 
GAGATATTGT GTAGAAAGGA CGCCGAGACC AAAATTGTTT CCAGGAAACA TCATAATACT 
AACGGATTAC CGAGAAAGAG GCAACAGAAG ACTCATTCTG AAAACTCTGA CTCTGAAATT 
GAACAGCATC ATCGCCTCCA TCATCATCAC CATCACCACC TTCACCATAG TAATGTATAT 
AAAAGGAACA GAGTAACTTC GCGAAAACGA ATTTGCGCGA AGAAACGGAC GAGAAAAACT 
GACATATCTT CAGATACAGT GTTCGAAGAT GAGAATTGTA AAAAGGATTC CTATTTAAGT 
ATTACATGTA GAAAGCAACA AACTATAAAA GGTACAAAAC GACGAGCCGA TACCATGTCA 
TCGGAAACAC AAAATTCTGA AGTCGATTGT GCATTATCGG GACATGTCAG TCCTACGATT 
ATAACTACTT CAGAACTTGA ACAAAGACGT TCGAGTATTG AGTTTCAACC AGTTGTTAGA 
ATGATGAAGA TTGACGATCA AGTTGAAATG GACCACAGTA TTCTCTCGGT AACGATTGCA 
AGCAACAGAC GGTTAAGAAG TTCCAGTTCT CCGAGATCGA ATACTCAACC GCCGAAAAAA 
CGTTTAAAGA CCAATCGTGG TCAATTCGGA AGGTGGTTAA AAAGTAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100745898 ( XM_024367821.1 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_024223589.1 Bombus impatiens uncharacterized LOC100745898 (LOC100745898), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBo104096
Clone ID Related Accession (Same CDS sequence)	XM_012385926.1 , XM_024367821.1 , XM_012385926.2 , XM_024367822.1
Accession Version	XM_024367821.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-18
Organism	Bombus impatiens(common eastern bumble bee)
Product	uncharacterized protein LOC100745898 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176780.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bombus impatiens Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAACTC CTCCAACATC ATTTTTTAGT CCTGAATGCC CCTTGTCTAA TACAACCAAT 
CTACGGGGTC AAACTACCCA AAGATCTAAA TCGCGGGTGG AGGCAATGTT GGAGCGTGCT 
CTGATACAGC CACAACAGTG TTCCGTGGAT CCACTCTACA ACGCTCAAAA TTGGGGAATT 
CCTATTTGGG CAATTGATAA ACTTCAGTCT TGGCTTGACA AAATCTACTC ATCTGTTAAA 
GACACGAATC ATTTGAAACA ATTAAGACAC TCACATCAAC AGAGTTCAAC TAAAGATATA 
AAGGTCAGAC ACCTCAAGGG TCCTTATTTG AAATTTGAAT CCTTTCAGAG GAATACTAGA 
CCTGTATTTG TTGAACTACC TGTGTGGCCA ACATTGAATT TTCACGGTGA TCCAGGCTCT 
TGTCCTTTCA ATACGAAGAG ACGAGAAAAA CTGGAAAAAT TAGGGAAAGA AGTAAAAGAA 
AACAGGGAAG GTATTCCGGC TATCAACCAA GAGGAGAAAA AAGAAATGAC TCGGCGACCA 
CGAGCAACTG TCCGCACTCG AAGAACAGAA CAATTAGCTT CCGGTTATTG TGAAATTTGC 
CGTATTAGAT ATAGAGATCT GGCCAAACAT GTGCAAAGCG ATCAGCATCT TAGCTTTATT 
CAAAATGATG ATAATTTCTT GTCTTTGGAT AAATTGATCA ATGCAGGAGC CAATGTAGAA 
GCGTTTTTAA AACTAAACAG AGGAAAAAAT ATAGAAAAAG ATTGCAATTT GTTTTCCAAT 
GGGGATCGTA ATCTTCATGA CACAGTATTG CCAGAAGGGA AGGTTAATAG AAATGCAAAA 
AGTTTAGGGG ATTTTGACGT TGGAGACATA AAGATGGTAC AACGTAATGG CGCACGTCGG 
AATCTCAGTT TAAGATTAAA CTCCTCTCAT AATTTACGTA CACGTACAAG ACACGAAAGC 
GGCCATTTAT TAAGATCAAA AGGTAGTCCC TGGCATGAAG CCGATAAAAC GGAGAAGTTC 
TACGACGAAT TCGAAGGTTT TACTATCAAA AAGCGAGCGA AAGGGACAAT TTGGATCGAA 
GAAGATGCAC CAGGAGACAA ATATATAGAA GAACATGAAT TAAAAGAATC TAAGCAAAAT 
GAATACAAAA TTAAAACATT TTCGGCGGAA TTAGAAGAAA AATGTTGTAA TGAGAAAAAT 
TGTGAAACAT ATAACAAACT TAAAGATACT AACAATCAGG ACATACAAAG GACAATAAAT 
TGTAATAACG AAGAAAATAG TATTGGAAAT GACCCTGAAC GCATCAAACA AGAACAAACT 
CATCCAAAGA GTAAAAATCA TGATCAAATT AATGGAAACA TAAAAAATGG TTGTAATGAA 
AATCTTTTAA GTACAAATGA TAACAGTCGT ATTAAACCTT GTAAAGTTTC GCCTGGTAAC 
GAAACTTTAA AAGAATTGAC TTGCAAATTA CAGTTAGAAA ATACTCCATC AAGTGCAAAA 
AGGTTATCAG AGCACAATGA TATCAATACA GGCACAATCT GTAATGGTCA TTCTGTAAGG 
GATGAACAGA AAATTAATAG AACACTGAAT AATACAGAAA AGAACGAAGT AGCAAAAGAA 
GAGAAATCAA AATGTTGCAA GTCAAATAGA AGAGGTAGTC GGAGCGTCAG AGGACGGCAC 
AGGTTGTCCG TTGAAGAACG TTTGATTGAA GATAATCGTG CATATTATAA AGTGGAAGTG 
TTAGGAAGTA AGTTAAGATC AAGTGTATTG TCAAACAATA ATTCTCAATG TACAGTTCAA 
AAGGATGGGG ATAGTGAAGA AAAGAAGGAA GTGCCATCTA GTGAGAAACC AGTAGTTGTA 
CGATTTAAAC GTGTAAGAAA GTCGGAACTA TCATTACTAA GCGATGAAGC GGAGTCTTTT 
ATGTTTAGGG AACTTAAACG AGATGATTCA AGTGAAATAA GCGATGACGA ACAGAGTAGC 
GTATTACCAA GGGATACTGA AAGTGAGTGC AACGATAGCG GCAATTCTAT CTGTCCTAGT 
TCATTCTTAA CTTCTAGTTC ACCTGCAAAA TCGGAAACTA CCGAAGACGA TTCTCAAGAC 
TCTTTAAGTT TGGGACGAGC TAGAAAAAAA AGACGGACAC AAGCAGAAGC GCTGATCAAA 
GATAATGTTG ATTATTACAA ATTCGAAACT CCTGGGAGCA GATTAAGGTA TCAGGCGCCT 
CTAACTGGTA TTAAAGAAAA TCAGGAAGAA ATAGAAGGTA GTATTGTCGA GAAAGGTATA 
GAAATAGCAA AGGTGTATCC GTCCAAACCC TCACCAGAAG TTGAAAAAAT GCAATTTTCT 
TTTGAAGCCG TACCAAAGTC TGAACCATGG TATCAAACGT ATCAGCGGCA AGATGAAGGA 
GCAGAATTTT GGCACTATTT TAGCGAAGGG GACTCACAAA AGCCATTTCT TTTACCATAT 
GAAATTGAAA ATTTTCACGA AATTTTGATA AAAAACCAAA ATAGAGCGGA AAATAAAAGA 
AAAGGTCGCG GACGGAGTAT AGGTTGTATT GGACGATCTC CTAGGAAAAG TCCTCGTTGT 
CATGCTTCCA CATTAGCTAT TATGTCAACA ATTATAAGAA AAAGAGAACA ACAGCAACAA 
CAATCTTCGA ATTTGTACAC AATAGATGAA TGCCAATCCA TCAGGTCACA TGCAAATACT 
CCCAGACCTG ATCAGAAAAC TGAGTTGAAA TCTGATGTGG ATGAAGATCT AAAAGAAATG 
GTAAAAAGTA TCGACGAGAT GCTTAATAAC GCAAACGATA GTTTCGAGTT GATTGAATCG 
TTCGAGCCTG ATACAACACA GATGGAATTG AATTTTTATG AACCAAATAT CCCAAGAGGA 
CCACCTCCAA ACTTGCTTGA ATTATTGGAC AGTTGTCACG ATATCGTAAA CTGTTTAGAA 
AATTCATCAT GTGCAAGTTC CGAGTGTGGT GAGGGAAGTG TCGAGTCACC TTTAAAACGA 
AGGAAGAAGA GGAAAAATAG AACCGGATGG CCTGGAATTA AAATGAAGAG AAGACTCCAA 
AGCAAACCTC TGGCAGACAT AAACTGTGAC AGGGAAAACC TAGTGGAGAA AAATATAGGG 
CAAAGCGAGA AGGATTGTAA TGTGCAGGTC ACGAATATGT CTAACAGATT AACGGAAGAA 
GGTAATATTA AAATAGCTAC ATCCGTTACG AGTTCCATCA CACAAGATGA ACAGTCATTA 
GGCTTCGGCC AGAGGAAGGA CGACGGACGT TTATTCGAAG TGTATACCTC GAATATTTCT 
TCTAACACGC GTCACGACGA AAACGAGAAG AAAGACGTAA GTATAGCGGT TCCTGATAAT 
TCCATGTTAC ATACAACAAA TGCTGAATCC TGCACAGGTT CACTTACATC TGATATATGC 
AACGAGACTG ACAGGAATTT CATATTGAAC AAAGACTATG GATTCAGAAA AAATTTGTCG 
AAGATCGAAG AAATGTCAGA AAATGATCAC GGAAACGATG AAAATCACGA GAACGTGTTT 
CGGAAAGATG TTCATTCTAT ATCGGAACGT CGTTTTATCT CGAACGCAAC CATCAAGAAA 
CGACAACATG ACAACACATC TTCTGAAACT TGCGAATCTC GTGTAGAACT CGAAAATCAT 
GAGATATTGT GTAGAAAGGA CGCCGAGACC AAAATTGTTT CCAGGAAACA TCATAATACT 
AACGGATTAC CGAGAAAGAG GCAACAGAAG ACTCATTCTG AAAACTCTGA CTCTGAAATT 
GAACAGCATC ATCGCCTCCA TCATCATCAC CATCACCACC TTCACCATAG TAATGTATAT 
AAAAGGAACA GAGTAACTTC GCGAAAACGA ATTTGCGCGA AGAAACGGAC GAGAAAAACT 
GACATATCTT CAGATACAGT GTTCGAAGAT GAGAATTGTA AAAAGGATTC CTATTTAAGT 
ATTACATGTA GAAAGCAACA AACTATAAAA GGTACAAAAC GACGAGCCGA TACCATGTCA 
TCGGAAACAC AAAATTCTGA AGTCGATTGT GCATTATCGG GACATGTCAG TCCTACGATT 
ATAACTACTT CAGAACTTGA ACAAAGACGT TCGAGTATTG AGTTTCAACC AGTTGTTAGA 
ATGATGAAGA TTGACGATCA AGTTGAAATG GACCACAGTA TTCTCTCGGT AACGATTGCA 
AGCAACAGAC GGTTAAGAAG TTCCAGTTCT CCGAGATCGA ATACTCAACC GCCGAAAAAA 
CGTTTAAAGA CCAATCGTGG TCAATTCGGA AGGTGGTTAA AAAGTAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024223589.1
CDS	646..4956
Translation	MPTPPTSFFSPECPLSNTTNLRGQTTQRSKSRVEAMLERALIQPQQCSVDPLYNAQNWGIPIWAIDKLQSWLDKIYSSVKDTNHLKQLRHSHQQSSTKDIKVRHLKGPYLKFESFQRNTRPVFVELPVWPTLNFHGDPGSCPFNTKRREKLEKLGKEVKENREGIPAINQEEKKEMTRRPRATVRTRRTEQLASGYCEICRIRYRDLAKHVQSDQHLSFIQNDDNFLSLDKLINAGANVEAFLKLNRGKNIEKDCNLFSNGDRNLHDTVLPEGKVNRNAKSLGDFDVGDIKMVQRNGARRNLSLRLNSSHNLRTRTRHESGHLLRSKGSPWHEADKTEKFYDEFEGFTIKKRAKGTIWIEEDAPGDKYIEEHELKESKQNEYKIKTFSAELEEKCCNEKNCETYNKLKDTNNQDIQRTINCNNEENSIGNDPERIKQEQTHPKSKNHDQINGNIKNGCNENLLSTNDNSRIKPCKVSPGNETLKELTCKLQLENTPSSAKRLSEHNDINTGTICNGHSVRDEQKINRTLNNTEKNEVAKEEKSKCCKSNRRGSRSVRGRHRLSVEERLIEDNRAYYKVEVLGSKLRSSVLSNNNSQCTVQKDGDSEEKKEVPSSEKPVVVRFKRVRKSELSLLSDEAESFMFRELKRDDSSEISDDEQSSVLPRDTESECNDSGNSICPSSFLTSSSPAKSETTEDDSQDSLSLGRARKKRRTQAEALIKDNVDYYKFETPGSRLRYQAPLTGIKENQEEIEGSIVEKGIEIAKVYPSKPSPEVEKMQFSFEAVPKSEPWYQTYQRQDEGAEFWHYFSEGDSQKPFLLPYEIENFHEILIKNQNRAENKRKGRGRSIGCIGRSPRKSPRCHASTLAIMSTIIRKREQQQQQSSNLYTIDECQSIRSHANTPRPDQKTELKSDVDEDLKEMVKSIDEMLNNANDSFELIESFEPDTTQMELNFYEPNIPRGPPPNLLELLDSCHDIVNCLENSSCASSECGEGSVESPLKRRKKRKNRTGWPGIKMKRRLQSKPLADINCDRENLVEKNIGQSEKDCNVQVTNMSNRLTEEGNIKIATSVTSSITQDEQSLGFGQRKDDGRLFEVYTSNISSNTRHDENEKKDVSIAVPDNSMLHTTNAESCTGSLTSDICNETDRNFILNKDYGFRKNLSKIEEMSENDHGNDENHENVFRKDVHSISERRFISNATIKKRQHDNTSSETCESRVELENHEILCRKDAETKIVSRKHHNTNGLPRKRQQKTHSENSDSEIEQHHRLHHHHHHHLHHSNVYKRNRVTSRKRICAKKRTRKTDISSDTVFEDENCKKDSYLSITCRKQQTIKGTKRRADTMSSETQNSEVDCALSGHVSPTIITTSELEQRRSSIEFQPVVRMMKIDDQVEMDHSILSVTIASNRRLRSSSSPRSNTQPPKKRLKTNRGQFGRWLKSS

Target ORF information:

RefSeq Version	XM_024367821.1
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens uncharacterized LOC100745898 (LOC100745898), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024367821.1

ORF Insert Sequence:

ATGCCAACTC CTCCAACATC ATTTTTTAGT CCTGAATGCC CCTTGTCTAA TACAACCAAT 
CTACGGGGTC AAACTACCCA AAGATCTAAA TCGCGGGTGG AGGCAATGTT GGAGCGTGCT 
CTGATACAGC CACAACAGTG TTCCGTGGAT CCACTCTACA ACGCTCAAAA TTGGGGAATT 
CCTATTTGGG CAATTGATAA ACTTCAGTCT TGGCTTGACA AAATCTACTC ATCTGTTAAA 
GACACGAATC ATTTGAAACA ATTAAGACAC TCACATCAAC AGAGTTCAAC TAAAGATATA 
AAGGTCAGAC ACCTCAAGGG TCCTTATTTG AAATTTGAAT CCTTTCAGAG GAATACTAGA 
CCTGTATTTG TTGAACTACC TGTGTGGCCA ACATTGAATT TTCACGGTGA TCCAGGCTCT 
TGTCCTTTCA ATACGAAGAG ACGAGAAAAA CTGGAAAAAT TAGGGAAAGA AGTAAAAGAA 
AACAGGGAAG GTATTCCGGC TATCAACCAA GAGGAGAAAA AAGAAATGAC TCGGCGACCA 
CGAGCAACTG TCCGCACTCG AAGAACAGAA CAATTAGCTT CCGGTTATTG TGAAATTTGC 
CGTATTAGAT ATAGAGATCT GGCCAAACAT GTGCAAAGCG ATCAGCATCT TAGCTTTATT 
CAAAATGATG ATAATTTCTT GTCTTTGGAT AAATTGATCA ATGCAGGAGC CAATGTAGAA 
GCGTTTTTAA AACTAAACAG AGGAAAAAAT ATAGAAAAAG ATTGCAATTT GTTTTCCAAT 
GGGGATCGTA ATCTTCATGA CACAGTATTG CCAGAAGGGA AGGTTAATAG AAATGCAAAA 
AGTTTAGGGG ATTTTGACGT TGGAGACATA AAGATGGTAC AACGTAATGG CGCACGTCGG 
AATCTCAGTT TAAGATTAAA CTCCTCTCAT AATTTACGTA CACGTACAAG ACACGAAAGC 
GGCCATTTAT TAAGATCAAA AGGTAGTCCC TGGCATGAAG CCGATAAAAC GGAGAAGTTC 
TACGACGAAT TCGAAGGTTT TACTATCAAA AAGCGAGCGA AAGGGACAAT TTGGATCGAA 
GAAGATGCAC CAGGAGACAA ATATATAGAA GAACATGAAT TAAAAGAATC TAAGCAAAAT 
GAATACAAAA TTAAAACATT TTCGGCGGAA TTAGAAGAAA AATGTTGTAA TGAGAAAAAT 
TGTGAAACAT ATAACAAACT TAAAGATACT AACAATCAGG ACATACAAAG GACAATAAAT 
TGTAATAACG AAGAAAATAG TATTGGAAAT GACCCTGAAC GCATCAAACA AGAACAAACT 
CATCCAAAGA GTAAAAATCA TGATCAAATT AATGGAAACA TAAAAAATGG TTGTAATGAA 
AATCTTTTAA GTACAAATGA TAACAGTCGT ATTAAACCTT GTAAAGTTTC GCCTGGTAAC 
GAAACTTTAA AAGAATTGAC TTGCAAATTA CAGTTAGAAA ATACTCCATC AAGTGCAAAA 
AGGTTATCAG AGCACAATGA TATCAATACA GGCACAATCT GTAATGGTCA TTCTGTAAGG 
GATGAACAGA AAATTAATAG AACACTGAAT AATACAGAAA AGAACGAAGT AGCAAAAGAA 
GAGAAATCAA AATGTTGCAA GTCAAATAGA AGAGGTAGTC GGAGCGTCAG AGGACGGCAC 
AGGTTGTCCG TTGAAGAACG TTTGATTGAA GATAATCGTG CATATTATAA AGTGGAAGTG 
TTAGGAAGTA AGTTAAGATC AAGTGTATTG TCAAACAATA ATTCTCAATG TACAGTTCAA 
AAGGATGGGG ATAGTGAAGA AAAGAAGGAA GTGCCATCTA GTGAGAAACC AGTAGTTGTA 
CGATTTAAAC GTGTAAGAAA GTCGGAACTA TCATTACTAA GCGATGAAGC GGAGTCTTTT 
ATGTTTAGGG AACTTAAACG AGATGATTCA AGTGAAATAA GCGATGACGA ACAGAGTAGC 
GTATTACCAA GGGATACTGA AAGTGAGTGC AACGATAGCG GCAATTCTAT CTGTCCTAGT 
TCATTCTTAA CTTCTAGTTC ACCTGCAAAA TCGGAAACTA CCGAAGACGA TTCTCAAGAC 
TCTTTAAGTT TGGGACGAGC TAGAAAAAAA AGACGGACAC AAGCAGAAGC GCTGATCAAA 
GATAATGTTG ATTATTACAA ATTCGAAACT CCTGGGAGCA GATTAAGGTA TCAGGCGCCT 
CTAACTGGTA TTAAAGAAAA TCAGGAAGAA ATAGAAGGTA GTATTGTCGA GAAAGGTATA 
GAAATAGCAA AGGTGTATCC GTCCAAACCC TCACCAGAAG TTGAAAAAAT GCAATTTTCT 
TTTGAAGCCG TACCAAAGTC TGAACCATGG TATCAAACGT ATCAGCGGCA AGATGAAGGA 
GCAGAATTTT GGCACTATTT TAGCGAAGGG GACTCACAAA AGCCATTTCT TTTACCATAT 
GAAATTGAAA ATTTTCACGA AATTTTGATA AAAAACCAAA ATAGAGCGGA AAATAAAAGA 
AAAGGTCGCG GACGGAGTAT AGGTTGTATT GGACGATCTC CTAGGAAAAG TCCTCGTTGT 
CATGCTTCCA CATTAGCTAT TATGTCAACA ATTATAAGAA AAAGAGAACA ACAGCAACAA 
CAATCTTCGA ATTTGTACAC AATAGATGAA TGCCAATCCA TCAGGTCACA TGCAAATACT 
CCCAGACCTG ATCAGAAAAC TGAGTTGAAA TCTGATGTGG ATGAAGATCT AAAAGAAATG 
GTAAAAAGTA TCGACGAGAT GCTTAATAAC GCAAACGATA GTTTCGAGTT GATTGAATCG 
TTCGAGCCTG ATACAACACA GATGGAATTG AATTTTTATG AACCAAATAT CCCAAGAGGA 
CCACCTCCAA ACTTGCTTGA ATTATTGGAC AGTTGTCACG ATATCGTAAA CTGTTTAGAA 
AATTCATCAT GTGCAAGTTC CGAGTGTGGT GAGGGAAGTG TCGAGTCACC TTTAAAACGA 
AGGAAGAAGA GGAAAAATAG AACCGGATGG CCTGGAATTA AAATGAAGAG AAGACTCCAA 
AGCAAACCTC TGGCAGACAT AAACTGTGAC AGGGAAAACC TAGTGGAGAA AAATATAGGG 
CAAAGCGAGA AGGATTGTAA TGTGCAGGTC ACGAATATGT CTAACAGATT AACGGAAGAA 
GGTAATATTA AAATAGCTAC ATCCGTTACG AGTTCCATCA CACAAGATGA ACAGTCATTA 
GGCTTCGGCC AGAGGAAGGA CGACGGACGT TTATTCGAAG TGTATACCTC GAATATTTCT 
TCTAACACGC GTCACGACGA AAACGAGAAG AAAGACGTAA GTATAGCGGT TCCTGATAAT 
TCCATGTTAC ATACAACAAA TGCTGAATCC TGCACAGGTT CACTTACATC TGATATATGC 
AACGAGACTG ACAGGAATTT CATATTGAAC AAAGACTATG GATTCAGAAA AAATTTGTCG 
AAGATCGAAG AAATGTCAGA AAATGATCAC GGAAACGATG AAAATCACGA GAACGTGTTT 
CGGAAAGATG TTCATTCTAT ATCGGAACGT CGTTTTATCT CGAACGCAAC CATCAAGAAA 
CGACAACATG ACAACACATC TTCTGAAACT TGCGAATCTC GTGTAGAACT CGAAAATCAT 
GAGATATTGT GTAGAAAGGA CGCCGAGACC AAAATTGTTT CCAGGAAACA TCATAATACT 
AACGGATTAC CGAGAAAGAG GCAACAGAAG ACTCATTCTG AAAACTCTGA CTCTGAAATT 
GAACAGCATC ATCGCCTCCA TCATCATCAC CATCACCACC TTCACCATAG TAATGTATAT 
AAAAGGAACA GAGTAACTTC GCGAAAACGA ATTTGCGCGA AGAAACGGAC GAGAAAAACT 
GACATATCTT CAGATACAGT GTTCGAAGAT GAGAATTGTA AAAAGGATTC CTATTTAAGT 
ATTACATGTA GAAAGCAACA AACTATAAAA GGTACAAAAC GACGAGCCGA TACCATGTCA 
TCGGAAACAC AAAATTCTGA AGTCGATTGT GCATTATCGG GACATGTCAG TCCTACGATT 
ATAACTACTT CAGAACTTGA ACAAAGACGT TCGAGTATTG AGTTTCAACC AGTTGTTAGA 
ATGATGAAGA TTGACGATCA AGTTGAAATG GACCACAGTA TTCTCTCGGT AACGATTGCA 
AGCAACAGAC GGTTAAGAAG TTCCAGTTCT CCGAGATCGA ATACTCAACC GCCGAAAAAA 
CGTTTAAAGA CCAATCGTGG TCAATTCGGA AGGTGGTTAA AAAGTAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100745898 ( XM_012385926.2 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_012241349.1 Bombus impatiens uncharacterized LOC100745898 (LOC100745898), transcript variant X2, mRNA.

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CloneID	OBo104096
Clone ID Related Accession (Same CDS sequence)	XM_012385926.1 , XM_024367821.1 , XM_012385926.2 , XM_024367822.1
Accession Version	XM_012385926.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-18
Organism	Bombus impatiens(common eastern bumble bee)
Product	uncharacterized protein LOC100745898 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176780.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 19, 2018 this sequence version replaced XM_012385926.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bombus impatiens Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAACTC CTCCAACATC ATTTTTTAGT CCTGAATGCC CCTTGTCTAA TACAACCAAT 
CTACGGGGTC AAACTACCCA AAGATCTAAA TCGCGGGTGG AGGCAATGTT GGAGCGTGCT 
CTGATACAGC CACAACAGTG TTCCGTGGAT CCACTCTACA ACGCTCAAAA TTGGGGAATT 
CCTATTTGGG CAATTGATAA ACTTCAGTCT TGGCTTGACA AAATCTACTC ATCTGTTAAA 
GACACGAATC ATTTGAAACA ATTAAGACAC TCACATCAAC AGAGTTCAAC TAAAGATATA 
AAGGTCAGAC ACCTCAAGGG TCCTTATTTG AAATTTGAAT CCTTTCAGAG GAATACTAGA 
CCTGTATTTG TTGAACTACC TGTGTGGCCA ACATTGAATT TTCACGGTGA TCCAGGCTCT 
TGTCCTTTCA ATACGAAGAG ACGAGAAAAA CTGGAAAAAT TAGGGAAAGA AGTAAAAGAA 
AACAGGGAAG GTATTCCGGC TATCAACCAA GAGGAGAAAA AAGAAATGAC TCGGCGACCA 
CGAGCAACTG TCCGCACTCG AAGAACAGAA CAATTAGCTT CCGGTTATTG TGAAATTTGC 
CGTATTAGAT ATAGAGATCT GGCCAAACAT GTGCAAAGCG ATCAGCATCT TAGCTTTATT 
CAAAATGATG ATAATTTCTT GTCTTTGGAT AAATTGATCA ATGCAGGAGC CAATGTAGAA 
GCGTTTTTAA AACTAAACAG AGGAAAAAAT ATAGAAAAAG ATTGCAATTT GTTTTCCAAT 
GGGGATCGTA ATCTTCATGA CACAGTATTG CCAGAAGGGA AGGTTAATAG AAATGCAAAA 
AGTTTAGGGG ATTTTGACGT TGGAGACATA AAGATGGTAC AACGTAATGG CGCACGTCGG 
AATCTCAGTT TAAGATTAAA CTCCTCTCAT AATTTACGTA CACGTACAAG ACACGAAAGC 
GGCCATTTAT TAAGATCAAA AGGTAGTCCC TGGCATGAAG CCGATAAAAC GGAGAAGTTC 
TACGACGAAT TCGAAGGTTT TACTATCAAA AAGCGAGCGA AAGGGACAAT TTGGATCGAA 
GAAGATGCAC CAGGAGACAA ATATATAGAA GAACATGAAT TAAAAGAATC TAAGCAAAAT 
GAATACAAAA TTAAAACATT TTCGGCGGAA TTAGAAGAAA AATGTTGTAA TGAGAAAAAT 
TGTGAAACAT ATAACAAACT TAAAGATACT AACAATCAGG ACATACAAAG GACAATAAAT 
TGTAATAACG AAGAAAATAG TATTGGAAAT GACCCTGAAC GCATCAAACA AGAACAAACT 
CATCCAAAGA GTAAAAATCA TGATCAAATT AATGGAAACA TAAAAAATGG TTGTAATGAA 
AATCTTTTAA GTACAAATGA TAACAGTCGT ATTAAACCTT GTAAAGTTTC GCCTGGTAAC 
GAAACTTTAA AAGAATTGAC TTGCAAATTA CAGTTAGAAA ATACTCCATC AAGTGCAAAA 
AGGTTATCAG AGCACAATGA TATCAATACA GGCACAATCT GTAATGGTCA TTCTGTAAGG 
GATGAACAGA AAATTAATAG AACACTGAAT AATACAGAAA AGAACGAAGT AGCAAAAGAA 
GAGAAATCAA AATGTTGCAA GTCAAATAGA AGAGGTAGTC GGAGCGTCAG AGGACGGCAC 
AGGTTGTCCG TTGAAGAACG TTTGATTGAA GATAATCGTG CATATTATAA AGTGGAAGTG 
TTAGGAAGTA AGTTAAGATC AAGTGTATTG TCAAACAATA ATTCTCAATG TACAGTTCAA 
AAGGATGGGG ATAGTGAAGA AAAGAAGGAA GTGCCATCTA GTGAGAAACC AGTAGTTGTA 
CGATTTAAAC GTGTAAGAAA GTCGGAACTA TCATTACTAA GCGATGAAGC GGAGTCTTTT 
ATGTTTAGGG AACTTAAACG AGATGATTCA AGTGAAATAA GCGATGACGA ACAGAGTAGC 
GTATTACCAA GGGATACTGA AAGTGAGTGC AACGATAGCG GCAATTCTAT CTGTCCTAGT 
TCATTCTTAA CTTCTAGTTC ACCTGCAAAA TCGGAAACTA CCGAAGACGA TTCTCAAGAC 
TCTTTAAGTT TGGGACGAGC TAGAAAAAAA AGACGGACAC AAGCAGAAGC GCTGATCAAA 
GATAATGTTG ATTATTACAA ATTCGAAACT CCTGGGAGCA GATTAAGGTA TCAGGCGCCT 
CTAACTGGTA TTAAAGAAAA TCAGGAAGAA ATAGAAGGTA GTATTGTCGA GAAAGGTATA 
GAAATAGCAA AGGTGTATCC GTCCAAACCC TCACCAGAAG TTGAAAAAAT GCAATTTTCT 
TTTGAAGCCG TACCAAAGTC TGAACCATGG TATCAAACGT ATCAGCGGCA AGATGAAGGA 
GCAGAATTTT GGCACTATTT TAGCGAAGGG GACTCACAAA AGCCATTTCT TTTACCATAT 
GAAATTGAAA ATTTTCACGA AATTTTGATA AAAAACCAAA ATAGAGCGGA AAATAAAAGA 
AAAGGTCGCG GACGGAGTAT AGGTTGTATT GGACGATCTC CTAGGAAAAG TCCTCGTTGT 
CATGCTTCCA CATTAGCTAT TATGTCAACA ATTATAAGAA AAAGAGAACA ACAGCAACAA 
CAATCTTCGA ATTTGTACAC AATAGATGAA TGCCAATCCA TCAGGTCACA TGCAAATACT 
CCCAGACCTG ATCAGAAAAC TGAGTTGAAA TCTGATGTGG ATGAAGATCT AAAAGAAATG 
GTAAAAAGTA TCGACGAGAT GCTTAATAAC GCAAACGATA GTTTCGAGTT GATTGAATCG 
TTCGAGCCTG ATACAACACA GATGGAATTG AATTTTTATG AACCAAATAT CCCAAGAGGA 
CCACCTCCAA ACTTGCTTGA ATTATTGGAC AGTTGTCACG ATATCGTAAA CTGTTTAGAA 
AATTCATCAT GTGCAAGTTC CGAGTGTGGT GAGGGAAGTG TCGAGTCACC TTTAAAACGA 
AGGAAGAAGA GGAAAAATAG AACCGGATGG CCTGGAATTA AAATGAAGAG AAGACTCCAA 
AGCAAACCTC TGGCAGACAT AAACTGTGAC AGGGAAAACC TAGTGGAGAA AAATATAGGG 
CAAAGCGAGA AGGATTGTAA TGTGCAGGTC ACGAATATGT CTAACAGATT AACGGAAGAA 
GGTAATATTA AAATAGCTAC ATCCGTTACG AGTTCCATCA CACAAGATGA ACAGTCATTA 
GGCTTCGGCC AGAGGAAGGA CGACGGACGT TTATTCGAAG TGTATACCTC GAATATTTCT 
TCTAACACGC GTCACGACGA AAACGAGAAG AAAGACGTAA GTATAGCGGT TCCTGATAAT 
TCCATGTTAC ATACAACAAA TGCTGAATCC TGCACAGGTT CACTTACATC TGATATATGC 
AACGAGACTG ACAGGAATTT CATATTGAAC AAAGACTATG GATTCAGAAA AAATTTGTCG 
AAGATCGAAG AAATGTCAGA AAATGATCAC GGAAACGATG AAAATCACGA GAACGTGTTT 
CGGAAAGATG TTCATTCTAT ATCGGAACGT CGTTTTATCT CGAACGCAAC CATCAAGAAA 
CGACAACATG ACAACACATC TTCTGAAACT TGCGAATCTC GTGTAGAACT CGAAAATCAT 
GAGATATTGT GTAGAAAGGA CGCCGAGACC AAAATTGTTT CCAGGAAACA TCATAATACT 
AACGGATTAC CGAGAAAGAG GCAACAGAAG ACTCATTCTG AAAACTCTGA CTCTGAAATT 
GAACAGCATC ATCGCCTCCA TCATCATCAC CATCACCACC TTCACCATAG TAATGTATAT 
AAAAGGAACA GAGTAACTTC GCGAAAACGA ATTTGCGCGA AGAAACGGAC GAGAAAAACT 
GACATATCTT CAGATACAGT GTTCGAAGAT GAGAATTGTA AAAAGGATTC CTATTTAAGT 
ATTACATGTA GAAAGCAACA AACTATAAAA GGTACAAAAC GACGAGCCGA TACCATGTCA 
TCGGAAACAC AAAATTCTGA AGTCGATTGT GCATTATCGG GACATGTCAG TCCTACGATT 
ATAACTACTT CAGAACTTGA ACAAAGACGT TCGAGTATTG AGTTTCAACC AGTTGTTAGA 
ATGATGAAGA TTGACGATCA AGTTGAAATG GACCACAGTA TTCTCTCGGT AACGATTGCA 
AGCAACAGAC GGTTAAGAAG TTCCAGTTCT CCGAGATCGA ATACTCAACC GCCGAAAAAA 
CGTTTAAAGA CCAATCGTGG TCAATTCGGA AGGTGGTTAA AAAGTAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012241349.1
CDS	736..5046
Translation	MPTPPTSFFSPECPLSNTTNLRGQTTQRSKSRVEAMLERALIQPQQCSVDPLYNAQNWGIPIWAIDKLQSWLDKIYSSVKDTNHLKQLRHSHQQSSTKDIKVRHLKGPYLKFESFQRNTRPVFVELPVWPTLNFHGDPGSCPFNTKRREKLEKLGKEVKENREGIPAINQEEKKEMTRRPRATVRTRRTEQLASGYCEICRIRYRDLAKHVQSDQHLSFIQNDDNFLSLDKLINAGANVEAFLKLNRGKNIEKDCNLFSNGDRNLHDTVLPEGKVNRNAKSLGDFDVGDIKMVQRNGARRNLSLRLNSSHNLRTRTRHESGHLLRSKGSPWHEADKTEKFYDEFEGFTIKKRAKGTIWIEEDAPGDKYIEEHELKESKQNEYKIKTFSAELEEKCCNEKNCETYNKLKDTNNQDIQRTINCNNEENSIGNDPERIKQEQTHPKSKNHDQINGNIKNGCNENLLSTNDNSRIKPCKVSPGNETLKELTCKLQLENTPSSAKRLSEHNDINTGTICNGHSVRDEQKINRTLNNTEKNEVAKEEKSKCCKSNRRGSRSVRGRHRLSVEERLIEDNRAYYKVEVLGSKLRSSVLSNNNSQCTVQKDGDSEEKKEVPSSEKPVVVRFKRVRKSELSLLSDEAESFMFRELKRDDSSEISDDEQSSVLPRDTESECNDSGNSICPSSFLTSSSPAKSETTEDDSQDSLSLGRARKKRRTQAEALIKDNVDYYKFETPGSRLRYQAPLTGIKENQEEIEGSIVEKGIEIAKVYPSKPSPEVEKMQFSFEAVPKSEPWYQTYQRQDEGAEFWHYFSEGDSQKPFLLPYEIENFHEILIKNQNRAENKRKGRGRSIGCIGRSPRKSPRCHASTLAIMSTIIRKREQQQQQSSNLYTIDECQSIRSHANTPRPDQKTELKSDVDEDLKEMVKSIDEMLNNANDSFELIESFEPDTTQMELNFYEPNIPRGPPPNLLELLDSCHDIVNCLENSSCASSECGEGSVESPLKRRKKRKNRTGWPGIKMKRRLQSKPLADINCDRENLVEKNIGQSEKDCNVQVTNMSNRLTEEGNIKIATSVTSSITQDEQSLGFGQRKDDGRLFEVYTSNISSNTRHDENEKKDVSIAVPDNSMLHTTNAESCTGSLTSDICNETDRNFILNKDYGFRKNLSKIEEMSENDHGNDENHENVFRKDVHSISERRFISNATIKKRQHDNTSSETCESRVELENHEILCRKDAETKIVSRKHHNTNGLPRKRQQKTHSENSDSEIEQHHRLHHHHHHHLHHSNVYKRNRVTSRKRICAKKRTRKTDISSDTVFEDENCKKDSYLSITCRKQQTIKGTKRRADTMSSETQNSEVDCALSGHVSPTIITTSELEQRRSSIEFQPVVRMMKIDDQVEMDHSILSVTIASNRRLRSSSSPRSNTQPPKKRLKTNRGQFGRWLKSS

Target ORF information:

RefSeq Version	XM_012385926.2
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens uncharacterized LOC100745898 (LOC100745898), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012385926.2

ORF Insert Sequence:

ATGCCAACTC CTCCAACATC ATTTTTTAGT CCTGAATGCC CCTTGTCTAA TACAACCAAT 
CTACGGGGTC AAACTACCCA AAGATCTAAA TCGCGGGTGG AGGCAATGTT GGAGCGTGCT 
CTGATACAGC CACAACAGTG TTCCGTGGAT CCACTCTACA ACGCTCAAAA TTGGGGAATT 
CCTATTTGGG CAATTGATAA ACTTCAGTCT TGGCTTGACA AAATCTACTC ATCTGTTAAA 
GACACGAATC ATTTGAAACA ATTAAGACAC TCACATCAAC AGAGTTCAAC TAAAGATATA 
AAGGTCAGAC ACCTCAAGGG TCCTTATTTG AAATTTGAAT CCTTTCAGAG GAATACTAGA 
CCTGTATTTG TTGAACTACC TGTGTGGCCA ACATTGAATT TTCACGGTGA TCCAGGCTCT 
TGTCCTTTCA ATACGAAGAG ACGAGAAAAA CTGGAAAAAT TAGGGAAAGA AGTAAAAGAA 
AACAGGGAAG GTATTCCGGC TATCAACCAA GAGGAGAAAA AAGAAATGAC TCGGCGACCA 
CGAGCAACTG TCCGCACTCG AAGAACAGAA CAATTAGCTT CCGGTTATTG TGAAATTTGC 
CGTATTAGAT ATAGAGATCT GGCCAAACAT GTGCAAAGCG ATCAGCATCT TAGCTTTATT 
CAAAATGATG ATAATTTCTT GTCTTTGGAT AAATTGATCA ATGCAGGAGC CAATGTAGAA 
GCGTTTTTAA AACTAAACAG AGGAAAAAAT ATAGAAAAAG ATTGCAATTT GTTTTCCAAT 
GGGGATCGTA ATCTTCATGA CACAGTATTG CCAGAAGGGA AGGTTAATAG AAATGCAAAA 
AGTTTAGGGG ATTTTGACGT TGGAGACATA AAGATGGTAC AACGTAATGG CGCACGTCGG 
AATCTCAGTT TAAGATTAAA CTCCTCTCAT AATTTACGTA CACGTACAAG ACACGAAAGC 
GGCCATTTAT TAAGATCAAA AGGTAGTCCC TGGCATGAAG CCGATAAAAC GGAGAAGTTC 
TACGACGAAT TCGAAGGTTT TACTATCAAA AAGCGAGCGA AAGGGACAAT TTGGATCGAA 
GAAGATGCAC CAGGAGACAA ATATATAGAA GAACATGAAT TAAAAGAATC TAAGCAAAAT 
GAATACAAAA TTAAAACATT TTCGGCGGAA TTAGAAGAAA AATGTTGTAA TGAGAAAAAT 
TGTGAAACAT ATAACAAACT TAAAGATACT AACAATCAGG ACATACAAAG GACAATAAAT 
TGTAATAACG AAGAAAATAG TATTGGAAAT GACCCTGAAC GCATCAAACA AGAACAAACT 
CATCCAAAGA GTAAAAATCA TGATCAAATT AATGGAAACA TAAAAAATGG TTGTAATGAA 
AATCTTTTAA GTACAAATGA TAACAGTCGT ATTAAACCTT GTAAAGTTTC GCCTGGTAAC 
GAAACTTTAA AAGAATTGAC TTGCAAATTA CAGTTAGAAA ATACTCCATC AAGTGCAAAA 
AGGTTATCAG AGCACAATGA TATCAATACA GGCACAATCT GTAATGGTCA TTCTGTAAGG 
GATGAACAGA AAATTAATAG AACACTGAAT AATACAGAAA AGAACGAAGT AGCAAAAGAA 
GAGAAATCAA AATGTTGCAA GTCAAATAGA AGAGGTAGTC GGAGCGTCAG AGGACGGCAC 
AGGTTGTCCG TTGAAGAACG TTTGATTGAA GATAATCGTG CATATTATAA AGTGGAAGTG 
TTAGGAAGTA AGTTAAGATC AAGTGTATTG TCAAACAATA ATTCTCAATG TACAGTTCAA 
AAGGATGGGG ATAGTGAAGA AAAGAAGGAA GTGCCATCTA GTGAGAAACC AGTAGTTGTA 
CGATTTAAAC GTGTAAGAAA GTCGGAACTA TCATTACTAA GCGATGAAGC GGAGTCTTTT 
ATGTTTAGGG AACTTAAACG AGATGATTCA AGTGAAATAA GCGATGACGA ACAGAGTAGC 
GTATTACCAA GGGATACTGA AAGTGAGTGC AACGATAGCG GCAATTCTAT CTGTCCTAGT 
TCATTCTTAA CTTCTAGTTC ACCTGCAAAA TCGGAAACTA CCGAAGACGA TTCTCAAGAC 
TCTTTAAGTT TGGGACGAGC TAGAAAAAAA AGACGGACAC AAGCAGAAGC GCTGATCAAA 
GATAATGTTG ATTATTACAA ATTCGAAACT CCTGGGAGCA GATTAAGGTA TCAGGCGCCT 
CTAACTGGTA TTAAAGAAAA TCAGGAAGAA ATAGAAGGTA GTATTGTCGA GAAAGGTATA 
GAAATAGCAA AGGTGTATCC GTCCAAACCC TCACCAGAAG TTGAAAAAAT GCAATTTTCT 
TTTGAAGCCG TACCAAAGTC TGAACCATGG TATCAAACGT ATCAGCGGCA AGATGAAGGA 
GCAGAATTTT GGCACTATTT TAGCGAAGGG GACTCACAAA AGCCATTTCT TTTACCATAT 
GAAATTGAAA ATTTTCACGA AATTTTGATA AAAAACCAAA ATAGAGCGGA AAATAAAAGA 
AAAGGTCGCG GACGGAGTAT AGGTTGTATT GGACGATCTC CTAGGAAAAG TCCTCGTTGT 
CATGCTTCCA CATTAGCTAT TATGTCAACA ATTATAAGAA AAAGAGAACA ACAGCAACAA 
CAATCTTCGA ATTTGTACAC AATAGATGAA TGCCAATCCA TCAGGTCACA TGCAAATACT 
CCCAGACCTG ATCAGAAAAC TGAGTTGAAA TCTGATGTGG ATGAAGATCT AAAAGAAATG 
GTAAAAAGTA TCGACGAGAT GCTTAATAAC GCAAACGATA GTTTCGAGTT GATTGAATCG 
TTCGAGCCTG ATACAACACA GATGGAATTG AATTTTTATG AACCAAATAT CCCAAGAGGA 
CCACCTCCAA ACTTGCTTGA ATTATTGGAC AGTTGTCACG ATATCGTAAA CTGTTTAGAA 
AATTCATCAT GTGCAAGTTC CGAGTGTGGT GAGGGAAGTG TCGAGTCACC TTTAAAACGA 
AGGAAGAAGA GGAAAAATAG AACCGGATGG CCTGGAATTA AAATGAAGAG AAGACTCCAA 
AGCAAACCTC TGGCAGACAT AAACTGTGAC AGGGAAAACC TAGTGGAGAA AAATATAGGG 
CAAAGCGAGA AGGATTGTAA TGTGCAGGTC ACGAATATGT CTAACAGATT AACGGAAGAA 
GGTAATATTA AAATAGCTAC ATCCGTTACG AGTTCCATCA CACAAGATGA ACAGTCATTA 
GGCTTCGGCC AGAGGAAGGA CGACGGACGT TTATTCGAAG TGTATACCTC GAATATTTCT 
TCTAACACGC GTCACGACGA AAACGAGAAG AAAGACGTAA GTATAGCGGT TCCTGATAAT 
TCCATGTTAC ATACAACAAA TGCTGAATCC TGCACAGGTT CACTTACATC TGATATATGC 
AACGAGACTG ACAGGAATTT CATATTGAAC AAAGACTATG GATTCAGAAA AAATTTGTCG 
AAGATCGAAG AAATGTCAGA AAATGATCAC GGAAACGATG AAAATCACGA GAACGTGTTT 
CGGAAAGATG TTCATTCTAT ATCGGAACGT CGTTTTATCT CGAACGCAAC CATCAAGAAA 
CGACAACATG ACAACACATC TTCTGAAACT TGCGAATCTC GTGTAGAACT CGAAAATCAT 
GAGATATTGT GTAGAAAGGA CGCCGAGACC AAAATTGTTT CCAGGAAACA TCATAATACT 
AACGGATTAC CGAGAAAGAG GCAACAGAAG ACTCATTCTG AAAACTCTGA CTCTGAAATT 
GAACAGCATC ATCGCCTCCA TCATCATCAC CATCACCACC TTCACCATAG TAATGTATAT 
AAAAGGAACA GAGTAACTTC GCGAAAACGA ATTTGCGCGA AGAAACGGAC GAGAAAAACT 
GACATATCTT CAGATACAGT GTTCGAAGAT GAGAATTGTA AAAAGGATTC CTATTTAAGT 
ATTACATGTA GAAAGCAACA AACTATAAAA GGTACAAAAC GACGAGCCGA TACCATGTCA 
TCGGAAACAC AAAATTCTGA AGTCGATTGT GCATTATCGG GACATGTCAG TCCTACGATT 
ATAACTACTT CAGAACTTGA ACAAAGACGT TCGAGTATTG AGTTTCAACC AGTTGTTAGA 
ATGATGAAGA TTGACGATCA AGTTGAAATG GACCACAGTA TTCTCTCGGT AACGATTGCA 
AGCAACAGAC GGTTAAGAAG TTCCAGTTCT CCGAGATCGA ATACTCAACC GCCGAAAAAA 
CGTTTAAAGA CCAATCGTGG TCAATTCGGA AGGTGGTTAA AAAGTAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100745898 ( XM_024367822.1 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_024223590.1 Bombus impatiens uncharacterized LOC100745898 (LOC100745898), transcript variant X4, mRNA.

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CloneID	OBo104096
Clone ID Related Accession (Same CDS sequence)	XM_012385926.1 , XM_024367821.1 , XM_012385926.2 , XM_024367822.1
Accession Version	XM_024367822.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-18
Organism	Bombus impatiens(common eastern bumble bee)
Product	uncharacterized protein LOC100745898 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176780.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bombus impatiens Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAACTC CTCCAACATC ATTTTTTAGT CCTGAATGCC CCTTGTCTAA TACAACCAAT 
CTACGGGGTC AAACTACCCA AAGATCTAAA TCGCGGGTGG AGGCAATGTT GGAGCGTGCT 
CTGATACAGC CACAACAGTG TTCCGTGGAT CCACTCTACA ACGCTCAAAA TTGGGGAATT 
CCTATTTGGG CAATTGATAA ACTTCAGTCT TGGCTTGACA AAATCTACTC ATCTGTTAAA 
GACACGAATC ATTTGAAACA ATTAAGACAC TCACATCAAC AGAGTTCAAC TAAAGATATA 
AAGGTCAGAC ACCTCAAGGG TCCTTATTTG AAATTTGAAT CCTTTCAGAG GAATACTAGA 
CCTGTATTTG TTGAACTACC TGTGTGGCCA ACATTGAATT TTCACGGTGA TCCAGGCTCT 
TGTCCTTTCA ATACGAAGAG ACGAGAAAAA CTGGAAAAAT TAGGGAAAGA AGTAAAAGAA 
AACAGGGAAG GTATTCCGGC TATCAACCAA GAGGAGAAAA AAGAAATGAC TCGGCGACCA 
CGAGCAACTG TCCGCACTCG AAGAACAGAA CAATTAGCTT CCGGTTATTG TGAAATTTGC 
CGTATTAGAT ATAGAGATCT GGCCAAACAT GTGCAAAGCG ATCAGCATCT TAGCTTTATT 
CAAAATGATG ATAATTTCTT GTCTTTGGAT AAATTGATCA ATGCAGGAGC CAATGTAGAA 
GCGTTTTTAA AACTAAACAG AGGAAAAAAT ATAGAAAAAG ATTGCAATTT GTTTTCCAAT 
GGGGATCGTA ATCTTCATGA CACAGTATTG CCAGAAGGGA AGGTTAATAG AAATGCAAAA 
AGTTTAGGGG ATTTTGACGT TGGAGACATA AAGATGGTAC AACGTAATGG CGCACGTCGG 
AATCTCAGTT TAAGATTAAA CTCCTCTCAT AATTTACGTA CACGTACAAG ACACGAAAGC 
GGCCATTTAT TAAGATCAAA AGGTAGTCCC TGGCATGAAG CCGATAAAAC GGAGAAGTTC 
TACGACGAAT TCGAAGGTTT TACTATCAAA AAGCGAGCGA AAGGGACAAT TTGGATCGAA 
GAAGATGCAC CAGGAGACAA ATATATAGAA GAACATGAAT TAAAAGAATC TAAGCAAAAT 
GAATACAAAA TTAAAACATT TTCGGCGGAA TTAGAAGAAA AATGTTGTAA TGAGAAAAAT 
TGTGAAACAT ATAACAAACT TAAAGATACT AACAATCAGG ACATACAAAG GACAATAAAT 
TGTAATAACG AAGAAAATAG TATTGGAAAT GACCCTGAAC GCATCAAACA AGAACAAACT 
CATCCAAAGA GTAAAAATCA TGATCAAATT AATGGAAACA TAAAAAATGG TTGTAATGAA 
AATCTTTTAA GTACAAATGA TAACAGTCGT ATTAAACCTT GTAAAGTTTC GCCTGGTAAC 
GAAACTTTAA AAGAATTGAC TTGCAAATTA CAGTTAGAAA ATACTCCATC AAGTGCAAAA 
AGGTTATCAG AGCACAATGA TATCAATACA GGCACAATCT GTAATGGTCA TTCTGTAAGG 
GATGAACAGA AAATTAATAG AACACTGAAT AATACAGAAA AGAACGAAGT AGCAAAAGAA 
GAGAAATCAA AATGTTGCAA GTCAAATAGA AGAGGTAGTC GGAGCGTCAG AGGACGGCAC 
AGGTTGTCCG TTGAAGAACG TTTGATTGAA GATAATCGTG CATATTATAA AGTGGAAGTG 
TTAGGAAGTA AGTTAAGATC AAGTGTATTG TCAAACAATA ATTCTCAATG TACAGTTCAA 
AAGGATGGGG ATAGTGAAGA AAAGAAGGAA GTGCCATCTA GTGAGAAACC AGTAGTTGTA 
CGATTTAAAC GTGTAAGAAA GTCGGAACTA TCATTACTAA GCGATGAAGC GGAGTCTTTT 
ATGTTTAGGG AACTTAAACG AGATGATTCA AGTGAAATAA GCGATGACGA ACAGAGTAGC 
GTATTACCAA GGGATACTGA AAGTGAGTGC AACGATAGCG GCAATTCTAT CTGTCCTAGT 
TCATTCTTAA CTTCTAGTTC ACCTGCAAAA TCGGAAACTA CCGAAGACGA TTCTCAAGAC 
TCTTTAAGTT TGGGACGAGC TAGAAAAAAA AGACGGACAC AAGCAGAAGC GCTGATCAAA 
GATAATGTTG ATTATTACAA ATTCGAAACT CCTGGGAGCA GATTAAGGTA TCAGGCGCCT 
CTAACTGGTA TTAAAGAAAA TCAGGAAGAA ATAGAAGGTA GTATTGTCGA GAAAGGTATA 
GAAATAGCAA AGGTGTATCC GTCCAAACCC TCACCAGAAG TTGAAAAAAT GCAATTTTCT 
TTTGAAGCCG TACCAAAGTC TGAACCATGG TATCAAACGT ATCAGCGGCA AGATGAAGGA 
GCAGAATTTT GGCACTATTT TAGCGAAGGG GACTCACAAA AGCCATTTCT TTTACCATAT 
GAAATTGAAA ATTTTCACGA AATTTTGATA AAAAACCAAA ATAGAGCGGA AAATAAAAGA 
AAAGGTCGCG GACGGAGTAT AGGTTGTATT GGACGATCTC CTAGGAAAAG TCCTCGTTGT 
CATGCTTCCA CATTAGCTAT TATGTCAACA ATTATAAGAA AAAGAGAACA ACAGCAACAA 
CAATCTTCGA ATTTGTACAC AATAGATGAA TGCCAATCCA TCAGGTCACA TGCAAATACT 
CCCAGACCTG ATCAGAAAAC TGAGTTGAAA TCTGATGTGG ATGAAGATCT AAAAGAAATG 
GTAAAAAGTA TCGACGAGAT GCTTAATAAC GCAAACGATA GTTTCGAGTT GATTGAATCG 
TTCGAGCCTG ATACAACACA GATGGAATTG AATTTTTATG AACCAAATAT CCCAAGAGGA 
CCACCTCCAA ACTTGCTTGA ATTATTGGAC AGTTGTCACG ATATCGTAAA CTGTTTAGAA 
AATTCATCAT GTGCAAGTTC CGAGTGTGGT GAGGGAAGTG TCGAGTCACC TTTAAAACGA 
AGGAAGAAGA GGAAAAATAG AACCGGATGG CCTGGAATTA AAATGAAGAG AAGACTCCAA 
AGCAAACCTC TGGCAGACAT AAACTGTGAC AGGGAAAACC TAGTGGAGAA AAATATAGGG 
CAAAGCGAGA AGGATTGTAA TGTGCAGGTC ACGAATATGT CTAACAGATT AACGGAAGAA 
GGTAATATTA AAATAGCTAC ATCCGTTACG AGTTCCATCA CACAAGATGA ACAGTCATTA 
GGCTTCGGCC AGAGGAAGGA CGACGGACGT TTATTCGAAG TGTATACCTC GAATATTTCT 
TCTAACACGC GTCACGACGA AAACGAGAAG AAAGACGTAA GTATAGCGGT TCCTGATAAT 
TCCATGTTAC ATACAACAAA TGCTGAATCC TGCACAGGTT CACTTACATC TGATATATGC 
AACGAGACTG ACAGGAATTT CATATTGAAC AAAGACTATG GATTCAGAAA AAATTTGTCG 
AAGATCGAAG AAATGTCAGA AAATGATCAC GGAAACGATG AAAATCACGA GAACGTGTTT 
CGGAAAGATG TTCATTCTAT ATCGGAACGT CGTTTTATCT CGAACGCAAC CATCAAGAAA 
CGACAACATG ACAACACATC TTCTGAAACT TGCGAATCTC GTGTAGAACT CGAAAATCAT 
GAGATATTGT GTAGAAAGGA CGCCGAGACC AAAATTGTTT CCAGGAAACA TCATAATACT 
AACGGATTAC CGAGAAAGAG GCAACAGAAG ACTCATTCTG AAAACTCTGA CTCTGAAATT 
GAACAGCATC ATCGCCTCCA TCATCATCAC CATCACCACC TTCACCATAG TAATGTATAT 
AAAAGGAACA GAGTAACTTC GCGAAAACGA ATTTGCGCGA AGAAACGGAC GAGAAAAACT 
GACATATCTT CAGATACAGT GTTCGAAGAT GAGAATTGTA AAAAGGATTC CTATTTAAGT 
ATTACATGTA GAAAGCAACA AACTATAAAA GGTACAAAAC GACGAGCCGA TACCATGTCA 
TCGGAAACAC AAAATTCTGA AGTCGATTGT GCATTATCGG GACATGTCAG TCCTACGATT 
ATAACTACTT CAGAACTTGA ACAAAGACGT TCGAGTATTG AGTTTCAACC AGTTGTTAGA 
ATGATGAAGA TTGACGATCA AGTTGAAATG GACCACAGTA TTCTCTCGGT AACGATTGCA 
AGCAACAGAC GGTTAAGAAG TTCCAGTTCT CCGAGATCGA ATACTCAACC GCCGAAAAAA 
CGTTTAAAGA CCAATCGTGG TCAATTCGGA AGGTGGTTAA AAAGTAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024223590.1
CDS	228..4538
Translation	MPTPPTSFFSPECPLSNTTNLRGQTTQRSKSRVEAMLERALIQPQQCSVDPLYNAQNWGIPIWAIDKLQSWLDKIYSSVKDTNHLKQLRHSHQQSSTKDIKVRHLKGPYLKFESFQRNTRPVFVELPVWPTLNFHGDPGSCPFNTKRREKLEKLGKEVKENREGIPAINQEEKKEMTRRPRATVRTRRTEQLASGYCEICRIRYRDLAKHVQSDQHLSFIQNDDNFLSLDKLINAGANVEAFLKLNRGKNIEKDCNLFSNGDRNLHDTVLPEGKVNRNAKSLGDFDVGDIKMVQRNGARRNLSLRLNSSHNLRTRTRHESGHLLRSKGSPWHEADKTEKFYDEFEGFTIKKRAKGTIWIEEDAPGDKYIEEHELKESKQNEYKIKTFSAELEEKCCNEKNCETYNKLKDTNNQDIQRTINCNNEENSIGNDPERIKQEQTHPKSKNHDQINGNIKNGCNENLLSTNDNSRIKPCKVSPGNETLKELTCKLQLENTPSSAKRLSEHNDINTGTICNGHSVRDEQKINRTLNNTEKNEVAKEEKSKCCKSNRRGSRSVRGRHRLSVEERLIEDNRAYYKVEVLGSKLRSSVLSNNNSQCTVQKDGDSEEKKEVPSSEKPVVVRFKRVRKSELSLLSDEAESFMFRELKRDDSSEISDDEQSSVLPRDTESECNDSGNSICPSSFLTSSSPAKSETTEDDSQDSLSLGRARKKRRTQAEALIKDNVDYYKFETPGSRLRYQAPLTGIKENQEEIEGSIVEKGIEIAKVYPSKPSPEVEKMQFSFEAVPKSEPWYQTYQRQDEGAEFWHYFSEGDSQKPFLLPYEIENFHEILIKNQNRAENKRKGRGRSIGCIGRSPRKSPRCHASTLAIMSTIIRKREQQQQQSSNLYTIDECQSIRSHANTPRPDQKTELKSDVDEDLKEMVKSIDEMLNNANDSFELIESFEPDTTQMELNFYEPNIPRGPPPNLLELLDSCHDIVNCLENSSCASSECGEGSVESPLKRRKKRKNRTGWPGIKMKRRLQSKPLADINCDRENLVEKNIGQSEKDCNVQVTNMSNRLTEEGNIKIATSVTSSITQDEQSLGFGQRKDDGRLFEVYTSNISSNTRHDENEKKDVSIAVPDNSMLHTTNAESCTGSLTSDICNETDRNFILNKDYGFRKNLSKIEEMSENDHGNDENHENVFRKDVHSISERRFISNATIKKRQHDNTSSETCESRVELENHEILCRKDAETKIVSRKHHNTNGLPRKRQQKTHSENSDSEIEQHHRLHHHHHHHLHHSNVYKRNRVTSRKRICAKKRTRKTDISSDTVFEDENCKKDSYLSITCRKQQTIKGTKRRADTMSSETQNSEVDCALSGHVSPTIITTSELEQRRSSIEFQPVVRMMKIDDQVEMDHSILSVTIASNRRLRSSSSPRSNTQPPKKRLKTNRGQFGRWLKSS

Target ORF information:

RefSeq Version	XM_024367822.1
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens uncharacterized LOC100745898 (LOC100745898), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024367822.1

ORF Insert Sequence:

ATGCCAACTC CTCCAACATC ATTTTTTAGT CCTGAATGCC CCTTGTCTAA TACAACCAAT 
CTACGGGGTC AAACTACCCA AAGATCTAAA TCGCGGGTGG AGGCAATGTT GGAGCGTGCT 
CTGATACAGC CACAACAGTG TTCCGTGGAT CCACTCTACA ACGCTCAAAA TTGGGGAATT 
CCTATTTGGG CAATTGATAA ACTTCAGTCT TGGCTTGACA AAATCTACTC ATCTGTTAAA 
GACACGAATC ATTTGAAACA ATTAAGACAC TCACATCAAC AGAGTTCAAC TAAAGATATA 
AAGGTCAGAC ACCTCAAGGG TCCTTATTTG AAATTTGAAT CCTTTCAGAG GAATACTAGA 
CCTGTATTTG TTGAACTACC TGTGTGGCCA ACATTGAATT TTCACGGTGA TCCAGGCTCT 
TGTCCTTTCA ATACGAAGAG ACGAGAAAAA CTGGAAAAAT TAGGGAAAGA AGTAAAAGAA 
AACAGGGAAG GTATTCCGGC TATCAACCAA GAGGAGAAAA AAGAAATGAC TCGGCGACCA 
CGAGCAACTG TCCGCACTCG AAGAACAGAA CAATTAGCTT CCGGTTATTG TGAAATTTGC 
CGTATTAGAT ATAGAGATCT GGCCAAACAT GTGCAAAGCG ATCAGCATCT TAGCTTTATT 
CAAAATGATG ATAATTTCTT GTCTTTGGAT AAATTGATCA ATGCAGGAGC CAATGTAGAA 
GCGTTTTTAA AACTAAACAG AGGAAAAAAT ATAGAAAAAG ATTGCAATTT GTTTTCCAAT 
GGGGATCGTA ATCTTCATGA CACAGTATTG CCAGAAGGGA AGGTTAATAG AAATGCAAAA 
AGTTTAGGGG ATTTTGACGT TGGAGACATA AAGATGGTAC AACGTAATGG CGCACGTCGG 
AATCTCAGTT TAAGATTAAA CTCCTCTCAT AATTTACGTA CACGTACAAG ACACGAAAGC 
GGCCATTTAT TAAGATCAAA AGGTAGTCCC TGGCATGAAG CCGATAAAAC GGAGAAGTTC 
TACGACGAAT TCGAAGGTTT TACTATCAAA AAGCGAGCGA AAGGGACAAT TTGGATCGAA 
GAAGATGCAC CAGGAGACAA ATATATAGAA GAACATGAAT TAAAAGAATC TAAGCAAAAT 
GAATACAAAA TTAAAACATT TTCGGCGGAA TTAGAAGAAA AATGTTGTAA TGAGAAAAAT 
TGTGAAACAT ATAACAAACT TAAAGATACT AACAATCAGG ACATACAAAG GACAATAAAT 
TGTAATAACG AAGAAAATAG TATTGGAAAT GACCCTGAAC GCATCAAACA AGAACAAACT 
CATCCAAAGA GTAAAAATCA TGATCAAATT AATGGAAACA TAAAAAATGG TTGTAATGAA 
AATCTTTTAA GTACAAATGA TAACAGTCGT ATTAAACCTT GTAAAGTTTC GCCTGGTAAC 
GAAACTTTAA AAGAATTGAC TTGCAAATTA CAGTTAGAAA ATACTCCATC AAGTGCAAAA 
AGGTTATCAG AGCACAATGA TATCAATACA GGCACAATCT GTAATGGTCA TTCTGTAAGG 
GATGAACAGA AAATTAATAG AACACTGAAT AATACAGAAA AGAACGAAGT AGCAAAAGAA 
GAGAAATCAA AATGTTGCAA GTCAAATAGA AGAGGTAGTC GGAGCGTCAG AGGACGGCAC 
AGGTTGTCCG TTGAAGAACG TTTGATTGAA GATAATCGTG CATATTATAA AGTGGAAGTG 
TTAGGAAGTA AGTTAAGATC AAGTGTATTG TCAAACAATA ATTCTCAATG TACAGTTCAA 
AAGGATGGGG ATAGTGAAGA AAAGAAGGAA GTGCCATCTA GTGAGAAACC AGTAGTTGTA 
CGATTTAAAC GTGTAAGAAA GTCGGAACTA TCATTACTAA GCGATGAAGC GGAGTCTTTT 
ATGTTTAGGG AACTTAAACG AGATGATTCA AGTGAAATAA GCGATGACGA ACAGAGTAGC 
GTATTACCAA GGGATACTGA AAGTGAGTGC AACGATAGCG GCAATTCTAT CTGTCCTAGT 
TCATTCTTAA CTTCTAGTTC ACCTGCAAAA TCGGAAACTA CCGAAGACGA TTCTCAAGAC 
TCTTTAAGTT TGGGACGAGC TAGAAAAAAA AGACGGACAC AAGCAGAAGC GCTGATCAAA 
GATAATGTTG ATTATTACAA ATTCGAAACT CCTGGGAGCA GATTAAGGTA TCAGGCGCCT 
CTAACTGGTA TTAAAGAAAA TCAGGAAGAA ATAGAAGGTA GTATTGTCGA GAAAGGTATA 
GAAATAGCAA AGGTGTATCC GTCCAAACCC TCACCAGAAG TTGAAAAAAT GCAATTTTCT 
TTTGAAGCCG TACCAAAGTC TGAACCATGG TATCAAACGT ATCAGCGGCA AGATGAAGGA 
GCAGAATTTT GGCACTATTT TAGCGAAGGG GACTCACAAA AGCCATTTCT TTTACCATAT 
GAAATTGAAA ATTTTCACGA AATTTTGATA AAAAACCAAA ATAGAGCGGA AAATAAAAGA 
AAAGGTCGCG GACGGAGTAT AGGTTGTATT GGACGATCTC CTAGGAAAAG TCCTCGTTGT 
CATGCTTCCA CATTAGCTAT TATGTCAACA ATTATAAGAA AAAGAGAACA ACAGCAACAA 
CAATCTTCGA ATTTGTACAC AATAGATGAA TGCCAATCCA TCAGGTCACA TGCAAATACT 
CCCAGACCTG ATCAGAAAAC TGAGTTGAAA TCTGATGTGG ATGAAGATCT AAAAGAAATG 
GTAAAAAGTA TCGACGAGAT GCTTAATAAC GCAAACGATA GTTTCGAGTT GATTGAATCG 
TTCGAGCCTG ATACAACACA GATGGAATTG AATTTTTATG AACCAAATAT CCCAAGAGGA 
CCACCTCCAA ACTTGCTTGA ATTATTGGAC AGTTGTCACG ATATCGTAAA CTGTTTAGAA 
AATTCATCAT GTGCAAGTTC CGAGTGTGGT GAGGGAAGTG TCGAGTCACC TTTAAAACGA 
AGGAAGAAGA GGAAAAATAG AACCGGATGG CCTGGAATTA AAATGAAGAG AAGACTCCAA 
AGCAAACCTC TGGCAGACAT AAACTGTGAC AGGGAAAACC TAGTGGAGAA AAATATAGGG 
CAAAGCGAGA AGGATTGTAA TGTGCAGGTC ACGAATATGT CTAACAGATT AACGGAAGAA 
GGTAATATTA AAATAGCTAC ATCCGTTACG AGTTCCATCA CACAAGATGA ACAGTCATTA 
GGCTTCGGCC AGAGGAAGGA CGACGGACGT TTATTCGAAG TGTATACCTC GAATATTTCT 
TCTAACACGC GTCACGACGA AAACGAGAAG AAAGACGTAA GTATAGCGGT TCCTGATAAT 
TCCATGTTAC ATACAACAAA TGCTGAATCC TGCACAGGTT CACTTACATC TGATATATGC 
AACGAGACTG ACAGGAATTT CATATTGAAC AAAGACTATG GATTCAGAAA AAATTTGTCG 
AAGATCGAAG AAATGTCAGA AAATGATCAC GGAAACGATG AAAATCACGA GAACGTGTTT 
CGGAAAGATG TTCATTCTAT ATCGGAACGT CGTTTTATCT CGAACGCAAC CATCAAGAAA 
CGACAACATG ACAACACATC TTCTGAAACT TGCGAATCTC GTGTAGAACT CGAAAATCAT 
GAGATATTGT GTAGAAAGGA CGCCGAGACC AAAATTGTTT CCAGGAAACA TCATAATACT 
AACGGATTAC CGAGAAAGAG GCAACAGAAG ACTCATTCTG AAAACTCTGA CTCTGAAATT 
GAACAGCATC ATCGCCTCCA TCATCATCAC CATCACCACC TTCACCATAG TAATGTATAT 
AAAAGGAACA GAGTAACTTC GCGAAAACGA ATTTGCGCGA AGAAACGGAC GAGAAAAACT 
GACATATCTT CAGATACAGT GTTCGAAGAT GAGAATTGTA AAAAGGATTC CTATTTAAGT 
ATTACATGTA GAAAGCAACA AACTATAAAA GGTACAAAAC GACGAGCCGA TACCATGTCA 
TCGGAAACAC AAAATTCTGA AGTCGATTGT GCATTATCGG GACATGTCAG TCCTACGATT 
ATAACTACTT CAGAACTTGA ACAAAGACGT TCGAGTATTG AGTTTCAACC AGTTGTTAGA 
ATGATGAAGA TTGACGATCA AGTTGAAATG GACCACAGTA TTCTCTCGGT AACGATTGCA 
AGCAACAGAC GGTTAAGAAG TTCCAGTTCT CCGAGATCGA ATACTCAACC GCCGAAAAAA 
CGTTTAAAGA CCAATCGTGG TCAATTCGGA AGGTGGTTAA AAAGTAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100745898 ( XM_012385927.1 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_012241350.1 Bombus impatiens uncharacterized LOC100745898 (LOC100745898), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBo104097
Clone ID Related Accession (Same CDS sequence)	XM_012385927.1 , XM_012385927.2
Accession Version	XM_012385927.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-27
Organism	Bombus impatiens(common eastern bumble bee)
Product	uncharacterized protein LOC100745898 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176780.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombus impatiens Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATCGC CATCCAAAAT TCAACATCAG GAGCATAAGC AGCCATCAAG AGAAAATAAA 
TTAAACAAAC ACTCACATGT TCATACAAAG AAGGAATTGA AATCATTGGA AGGAAAGTCG 
TTTTACTTGG ATATCAAGAA TCATGCTACT ACAACTAAAA TAGAAGCTAA GATTAAAGAT 
TTGGGAGGAG TAATAGAATT ATTTTTAATA CGGAGTGTAG ACCTGGTGAT TAGTGACAGA 
GTAGACAAGA CTGGTTACAT AAATTTTGAA AAACATAAAT GGGGTTGTGC CAGTGGAGGC 
AGCGGGGGAC CTCCCTCGCT GAGGAGTTTA GAAACTCCAA CCCCCATGCC AACTCCTCCA 
ACATCATTTT TTAGTCCTGA ATGCCCCTTG TCTAATACAA CCAATCTACG GGGTCAAACT 
ACCCAAAGAT CTAAATCGCG GGTGGAGGCA ATGTTGGAGC GTGCTCTGAT ACAGCCACAA 
CAGTGTTCCG TGGATCCACT CTACAACGCT CAAAATTGGG GAATTCCTAT TTGGGCAATT 
GATAAACTTC AGTCTTGGCT TGACAAAATC TACTCATCTG TTAAAGACAC GAATCATTTG 
AAACAATTAA GACACTCACA TCAACAGAGT TCAACTAAAG ATATAAAGGT CAGACACCTC 
AAGGGTCCTT ATTTGAAATT TGAATCCTTT CAGAGGAATA CTAGACCTGT ATTTGTTGAA 
CTACCTGTGT GGCCAACATT GAATTTTCAC GGTGATCCAG GCTCTTGTCC TTTCAATACG 
AAGAGACGAG AAAAACTGGA AAAATTAGGG AAAGAAGTAA AAGAAAACAG GGAAGGTATT 
CCGGCTATCA ACCAAGAGGA GAAAAAAGAA ATGACTCGGC GACCACGAGC AACTGTCCGC 
ACTCGAAGAA CAGAACAATT AGCTTCCGGT TATTGTGAAA TTTGCCGTAT TAGATATAGA 
GATCTGGCCA AACATGTGCA AAGCGATCAG CATCTTAGCT TTATTCAAAA TGATGATAAT 
TTCTTGTCTT TGGATAAATT GATCAATGCA GGAGCCAATG TAGAAGCGTT TTTAAAACTA 
AACAGAGGAA AAAATATAGA AAAAGATTGC AATTTGTTTT CCAATGGGGA TCGTAATCTT 
CATGACACAG TATTGCCAGA AGGGAAGGTT AATAGAAATG CAAAAAGTTT AGGGGATTTT 
GACGTTGGAG ACATAAAGAT GGTACAACGT AATGGCGCAC GTCGGAATCT CAGTTTAAGA 
TTAAACTCCT CTCATAATTT ACGTACACGT ACAAGACACG AAAGCGGCCA TTTATTAAGA 
TCAAAAGGTA GTCCCTGGCA TGAAGCCGAT AAAACGGAGA AGTTCTACGA CGAATTCGAA 
GGTTTTACTA TCAAAAAGCG AGCGAAAGGG ACAATTTGGA TCGAAGAAGA TGCACCAGGA 
GACAAATATA TAGAAGAACA TGAATTAAAA GAATCTAAGC AAAATGAATA CAAAATTAAA 
ACATTTTCGG CGGAATTAGA AGAAAAATGT TGTAATGAGA AAAATTGTGA AACATATAAC 
AAACTTAAAG ATACTAACAA TCAGGACATA CAAAGGACAA TAAATTGTAA TAACGAAGAA 
AATAGTATTG GAAATGACCC TGAACGCATC AAACAAGAAC AAACTCATCC AAAGAGTAAA 
AATCATGATC AAATTAATGG AAACATAAAA AATGGTTGTA ATGAAAATCT TTTAAGTACA 
AATGATAACA GTCGTATTAA ACCTTGTAAA GTTTCGCCTG GTAACGAAAC TTTAAAAGAA 
TTGACTTGCA AATTACAGTT AGAAAATACT CCATCAAGTG CAAAAAGGTT ATCAGAGCAC 
AATGATATCA ATACAGGCAC AATCTGTAAT GGTCATTCTG TAAGGGATGA ACAGAAAATT 
AATAGAACAC TGAATAATAC AGAAAAGAAC GAAGTAGCAA AAGAAGAGAA ATCAAAATGT 
TGCAAGTCAA ATAGAAGAGG TAGTCGGAGC GTCAGAGGAC GGCACAGGTT GTCCGTTGAA 
GAACGTTTGA TTGAAGATAA TCGTGCATAT TATAAAGTGG AAGTGTTAGG AAGTAAGTTA 
AGATCAAGTG TATTGTCAAA CAATAATTCT CAATGTACAG TTCAAAAGGA TGGGGATAGT 
GAAGAAAAGA AGGAAGTGCC ATCTAGTGAG AAACCAGTAG TTGTACGATT TAAACGTGTA 
AGAAAGTCGG AACTATCATT ACTAAGCGAT GAAGCGGAGT CTTTTATGTT TAGGGAACTT 
AAACGAGATG ATTCAAGTGA AATAAGCGAT GACGAACAGA GTAGCGTATT ACCAAGGGAT 
ACTGAAAGTG AGTGCAACGA TAGCGGCAAT TCTATCTGTC CTAGTTCATT CTTAACTTCT 
AGTTCACCTG CAAAATCGGA AACTACCGAA GACGATTCTC AAGACTCTTT AAGTTTGGGA 
CGAGCTAGAA AAAAAAGACG GACACAAGCA GAAGCGCTGA TCAAAGATAA TGTTGATTAT 
TACAAATTCG AAACTCCTGG GAGCAGATTA AGGTATCAGG CGCCTCTAAC TGGTATTAAA 
GAAAATCAGG AAGAAATAGA AGGTAGTATT GTCGAGAAAG GTATAGAAAT AGCAAAGGTG 
TATCCGTCCA AACCCTCACC AGAAGTTGAA AAAATGCAAT TTTCTTTTGA AGCCGTACCA 
AAGTCTGAAC CATGGTATCA AACGTATCAG CGGCAAGATG AAGGAGCAGA ATTTTGGCAC 
TATTTTAGCG AAGGGGACTC ACAAAAGCCA TTTCTTTTAC CATATGAAAT TGAAAATTTT 
CACGAAATTT TGATAAAAAA CCAAAATAGA GCGGAAAATA AAAGAAAAGG TCGCGGACGG 
AGTATAGGTT GTATTGGACG ATCTCCTAGG AAAAGTCCTC GTTGTCATGC TTCCACATTA 
GCTATTATGT CAACAATTAT AAGAAAAAGA GAACAACAGC AACAACAATC TTCGAATTTG 
TACACAATAG ATGAATGCCA ATCCATCAGG TCACATGCAA ATACTCCCAG ACCTGATCAG 
AAAACTGAGT TGAAATCTGA TGTGGATGAA GATCTAAAAG AAATGGTAAA AAGTATCGAC 
GAGATGCTTA ATAACGCAAA CGATAGTTTC GAGTTGATTG AATCGTTCGA GCCTGATACA 
ACACAGATGG AATTGAATTT TTATGAACCA AATATCCCAA GAGGACCACC TCCAAACTTG 
CTTGAATTAT TGGACAGTTG TCACGATATC GTAAACTGTT TAGAAAATTC ATCATGTGCA 
AGTTCCGAGT GTGGTGAGGG AAGTGTCGAG TCACCTTTAA AACGAAGGAA GAAGAGGAAA 
AATAGAACCG GATGGCCTGG AATTAAAATG AAGAGAAGAC TCCAAAGCAA ACCTCTGGCA 
GACATAAACT GTGACAGGGA AAACCTAGTG GAGAAAAATA TAGGGCAAAG CGAGAAGGAT 
TGTAATGTGC AGGTCACGAA TATGTCTAAC AGATTAACGG AAGAAGGTAA TATTAAAATA 
GCTACATCCG TTACGAGTTC CATCACACAA GATGAACAGT CATTAGGCTT CGGCCAGAGG 
AAGGACGACG GACGTTTATT CGAAGTGTAT ACCTCGAATA TTTCTTCTAA CACGCGTCAC 
GACGAAAACG AGAAGAAAGA CGTTCACTTA CATCTGATAT ATGCAACGAG ACTGACAGGA 
ATTTCATATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012241350.1
CDS	242..3973
Translation	MVSPSKIQHQEHKQPSRENKLNKHSHVHTKKELKSLEGKSFYLDIKNHATTTKIEAKIKDLGGVIELFLIRSVDLVISDRVDKTGYINFEKHKWGCASGGSGGPPSLRSLETPTPMPTPPTSFFSPECPLSNTTNLRGQTTQRSKSRVEAMLERALIQPQQCSVDPLYNAQNWGIPIWAIDKLQSWLDKIYSSVKDTNHLKQLRHSHQQSSTKDIKVRHLKGPYLKFESFQRNTRPVFVELPVWPTLNFHGDPGSCPFNTKRREKLEKLGKEVKENREGIPAINQEEKKEMTRRPRATVRTRRTEQLASGYCEICRIRYRDLAKHVQSDQHLSFIQNDDNFLSLDKLINAGANVEAFLKLNRGKNIEKDCNLFSNGDRNLHDTVLPEGKVNRNAKSLGDFDVGDIKMVQRNGARRNLSLRLNSSHNLRTRTRHESGHLLRSKGSPWHEADKTEKFYDEFEGFTIKKRAKGTIWIEEDAPGDKYIEEHELKESKQNEYKIKTFSAELEEKCCNEKNCETYNKLKDTNNQDIQRTINCNNEENSIGNDPERIKQEQTHPKSKNHDQINGNIKNGCNENLLSTNDNSRIKPCKVSPGNETLKELTCKLQLENTPSSAKRLSEHNDINTGTICNGHSVRDEQKINRTLNNTEKNEVAKEEKSKCCKSNRRGSRSVRGRHRLSVEERLIEDNRAYYKVEVLGSKLRSSVLSNNNSQCTVQKDGDSEEKKEVPSSEKPVVVRFKRVRKSELSLLSDEAESFMFRELKRDDSSEISDDEQSSVLPRDTESECNDSGNSICPSSFLTSSSPAKSETTEDDSQDSLSLGRARKKRRTQAEALIKDNVDYYKFETPGSRLRYQAPLTGIKENQEEIEGSIVEKGIEIAKVYPSKPSPEVEKMQFSFEAVPKSEPWYQTYQRQDEGAEFWHYFSEGDSQKPFLLPYEIENFHEILIKNQNRAENKRKGRGRSIGCIGRSPRKSPRCHASTLAIMSTIIRKREQQQQQSSNLYTIDECQSIRSHANTPRPDQKTELKSDVDEDLKEMVKSIDEMLNNANDSFELIESFEPDTTQMELNFYEPNIPRGPPPNLLELLDSCHDIVNCLENSSCASSECGEGSVESPLKRRKKRKNRTGWPGIKMKRRLQSKPLADINCDRENLVEKNIGQSEKDCNVQVTNMSNRLTEEGNIKIATSVTSSITQDEQSLGFGQRKDDGRLFEVYTSNISSNTRHDENEKKDVHLHLIYATRLTGISY

Target ORF information:

RefSeq Version	XM_012385927.1
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens uncharacterized LOC100745898 (LOC100745898), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012385927.1

ORF Insert Sequence:

ATGGTATCGC CATCCAAAAT TCAACATCAG GAGCATAAGC AGCCATCAAG AGAAAATAAA 
TTAAACAAAC ACTCACATGT TCATACAAAG AAGGAATTGA AATCATTGGA AGGAAAGTCG 
TTTTACTTGG ATATCAAGAA TCATGCTACT ACAACTAAAA TAGAAGCTAA GATTAAAGAT 
TTGGGAGGAG TAATAGAATT ATTTTTAATA CGGAGTGTAG ACCTGGTGAT TAGTGACAGA 
GTAGACAAGA CTGGTTACAT AAATTTTGAA AAACATAAAT GGGGTTGTGC CAGTGGAGGC 
AGCGGGGGAC CTCCCTCGCT GAGGAGTTTA GAAACTCCAA CCCCCATGCC AACTCCTCCA 
ACATCATTTT TTAGTCCTGA ATGCCCCTTG TCTAATACAA CCAATCTACG GGGTCAAACT 
ACCCAAAGAT CTAAATCGCG GGTGGAGGCA ATGTTGGAGC GTGCTCTGAT ACAGCCACAA 
CAGTGTTCCG TGGATCCACT CTACAACGCT CAAAATTGGG GAATTCCTAT TTGGGCAATT 
GATAAACTTC AGTCTTGGCT TGACAAAATC TACTCATCTG TTAAAGACAC GAATCATTTG 
AAACAATTAA GACACTCACA TCAACAGAGT TCAACTAAAG ATATAAAGGT CAGACACCTC 
AAGGGTCCTT ATTTGAAATT TGAATCCTTT CAGAGGAATA CTAGACCTGT ATTTGTTGAA 
CTACCTGTGT GGCCAACATT GAATTTTCAC GGTGATCCAG GCTCTTGTCC TTTCAATACG 
AAGAGACGAG AAAAACTGGA AAAATTAGGG AAAGAAGTAA AAGAAAACAG GGAAGGTATT 
CCGGCTATCA ACCAAGAGGA GAAAAAAGAA ATGACTCGGC GACCACGAGC AACTGTCCGC 
ACTCGAAGAA CAGAACAATT AGCTTCCGGT TATTGTGAAA TTTGCCGTAT TAGATATAGA 
GATCTGGCCA AACATGTGCA AAGCGATCAG CATCTTAGCT TTATTCAAAA TGATGATAAT 
TTCTTGTCTT TGGATAAATT GATCAATGCA GGAGCCAATG TAGAAGCGTT TTTAAAACTA 
AACAGAGGAA AAAATATAGA AAAAGATTGC AATTTGTTTT CCAATGGGGA TCGTAATCTT 
CATGACACAG TATTGCCAGA AGGGAAGGTT AATAGAAATG CAAAAAGTTT AGGGGATTTT 
GACGTTGGAG ACATAAAGAT GGTACAACGT AATGGCGCAC GTCGGAATCT CAGTTTAAGA 
TTAAACTCCT CTCATAATTT ACGTACACGT ACAAGACACG AAAGCGGCCA TTTATTAAGA 
TCAAAAGGTA GTCCCTGGCA TGAAGCCGAT AAAACGGAGA AGTTCTACGA CGAATTCGAA 
GGTTTTACTA TCAAAAAGCG AGCGAAAGGG ACAATTTGGA TCGAAGAAGA TGCACCAGGA 
GACAAATATA TAGAAGAACA TGAATTAAAA GAATCTAAGC AAAATGAATA CAAAATTAAA 
ACATTTTCGG CGGAATTAGA AGAAAAATGT TGTAATGAGA AAAATTGTGA AACATATAAC 
AAACTTAAAG ATACTAACAA TCAGGACATA CAAAGGACAA TAAATTGTAA TAACGAAGAA 
AATAGTATTG GAAATGACCC TGAACGCATC AAACAAGAAC AAACTCATCC AAAGAGTAAA 
AATCATGATC AAATTAATGG AAACATAAAA AATGGTTGTA ATGAAAATCT TTTAAGTACA 
AATGATAACA GTCGTATTAA ACCTTGTAAA GTTTCGCCTG GTAACGAAAC TTTAAAAGAA 
TTGACTTGCA AATTACAGTT AGAAAATACT CCATCAAGTG CAAAAAGGTT ATCAGAGCAC 
AATGATATCA ATACAGGCAC AATCTGTAAT GGTCATTCTG TAAGGGATGA ACAGAAAATT 
AATAGAACAC TGAATAATAC AGAAAAGAAC GAAGTAGCAA AAGAAGAGAA ATCAAAATGT 
TGCAAGTCAA ATAGAAGAGG TAGTCGGAGC GTCAGAGGAC GGCACAGGTT GTCCGTTGAA 
GAACGTTTGA TTGAAGATAA TCGTGCATAT TATAAAGTGG AAGTGTTAGG AAGTAAGTTA 
AGATCAAGTG TATTGTCAAA CAATAATTCT CAATGTACAG TTCAAAAGGA TGGGGATAGT 
GAAGAAAAGA AGGAAGTGCC ATCTAGTGAG AAACCAGTAG TTGTACGATT TAAACGTGTA 
AGAAAGTCGG AACTATCATT ACTAAGCGAT GAAGCGGAGT CTTTTATGTT TAGGGAACTT 
AAACGAGATG ATTCAAGTGA AATAAGCGAT GACGAACAGA GTAGCGTATT ACCAAGGGAT 
ACTGAAAGTG AGTGCAACGA TAGCGGCAAT TCTATCTGTC CTAGTTCATT CTTAACTTCT 
AGTTCACCTG CAAAATCGGA AACTACCGAA GACGATTCTC AAGACTCTTT AAGTTTGGGA 
CGAGCTAGAA AAAAAAGACG GACACAAGCA GAAGCGCTGA TCAAAGATAA TGTTGATTAT 
TACAAATTCG AAACTCCTGG GAGCAGATTA AGGTATCAGG CGCCTCTAAC TGGTATTAAA 
GAAAATCAGG AAGAAATAGA AGGTAGTATT GTCGAGAAAG GTATAGAAAT AGCAAAGGTG 
TATCCGTCCA AACCCTCACC AGAAGTTGAA AAAATGCAAT TTTCTTTTGA AGCCGTACCA 
AAGTCTGAAC CATGGTATCA AACGTATCAG CGGCAAGATG AAGGAGCAGA ATTTTGGCAC 
TATTTTAGCG AAGGGGACTC ACAAAAGCCA TTTCTTTTAC CATATGAAAT TGAAAATTTT 
CACGAAATTT TGATAAAAAA CCAAAATAGA GCGGAAAATA AAAGAAAAGG TCGCGGACGG 
AGTATAGGTT GTATTGGACG ATCTCCTAGG AAAAGTCCTC GTTGTCATGC TTCCACATTA 
GCTATTATGT CAACAATTAT AAGAAAAAGA GAACAACAGC AACAACAATC TTCGAATTTG 
TACACAATAG ATGAATGCCA ATCCATCAGG TCACATGCAA ATACTCCCAG ACCTGATCAG 
AAAACTGAGT TGAAATCTGA TGTGGATGAA GATCTAAAAG AAATGGTAAA AAGTATCGAC 
GAGATGCTTA ATAACGCAAA CGATAGTTTC GAGTTGATTG AATCGTTCGA GCCTGATACA 
ACACAGATGG AATTGAATTT TTATGAACCA AATATCCCAA GAGGACCACC TCCAAACTTG 
CTTGAATTAT TGGACAGTTG TCACGATATC GTAAACTGTT TAGAAAATTC ATCATGTGCA 
AGTTCCGAGT GTGGTGAGGG AAGTGTCGAG TCACCTTTAA AACGAAGGAA GAAGAGGAAA 
AATAGAACCG GATGGCCTGG AATTAAAATG AAGAGAAGAC TCCAAAGCAA ACCTCTGGCA 
GACATAAACT GTGACAGGGA AAACCTAGTG GAGAAAAATA TAGGGCAAAG CGAGAAGGAT 
TGTAATGTGC AGGTCACGAA TATGTCTAAC AGATTAACGG AAGAAGGTAA TATTAAAATA 
GCTACATCCG TTACGAGTTC CATCACACAA GATGAACAGT CATTAGGCTT CGGCCAGAGG 
AAGGACGACG GACGTTTATT CGAAGTGTAT ACCTCGAATA TTTCTTCTAA CACGCGTCAC 
GACGAAAACG AGAAGAAAGA CGTTCACTTA CATCTGATAT ATGCAACGAG ACTGACAGGA 
ATTTCATATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100745898 ( XM_012385927.2 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_012241350.1 Bombus impatiens uncharacterized LOC100745898 (LOC100745898), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBo104097
Clone ID Related Accession (Same CDS sequence)	XM_012385927.1 , XM_012385927.2
Accession Version	XM_012385927.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-18
Organism	Bombus impatiens(common eastern bumble bee)
Product	uncharacterized protein LOC100745898 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176780.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 19, 2018 this sequence version replaced XM_012385927.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bombus impatiens Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATCGC CATCCAAAAT TCAACATCAG GAGCATAAGC AGCCATCAAG AGAAAATAAA 
TTAAACAAAC ACTCACATGT TCATACAAAG AAGGAATTGA AATCATTGGA AGGAAAGTCG 
TTTTACTTGG ATATCAAGAA TCATGCTACT ACAACTAAAA TAGAAGCTAA GATTAAAGAT 
TTGGGAGGAG TAATAGAATT ATTTTTAATA CGGAGTGTAG ACCTGGTGAT TAGTGACAGA 
GTAGACAAGA CTGGTTACAT AAATTTTGAA AAACATAAAT GGGGTTGTGC CAGTGGAGGC 
AGCGGGGGAC CTCCCTCGCT GAGGAGTTTA GAAACTCCAA CCCCCATGCC AACTCCTCCA 
ACATCATTTT TTAGTCCTGA ATGCCCCTTG TCTAATACAA CCAATCTACG GGGTCAAACT 
ACCCAAAGAT CTAAATCGCG GGTGGAGGCA ATGTTGGAGC GTGCTCTGAT ACAGCCACAA 
CAGTGTTCCG TGGATCCACT CTACAACGCT CAAAATTGGG GAATTCCTAT TTGGGCAATT 
GATAAACTTC AGTCTTGGCT TGACAAAATC TACTCATCTG TTAAAGACAC GAATCATTTG 
AAACAATTAA GACACTCACA TCAACAGAGT TCAACTAAAG ATATAAAGGT CAGACACCTC 
AAGGGTCCTT ATTTGAAATT TGAATCCTTT CAGAGGAATA CTAGACCTGT ATTTGTTGAA 
CTACCTGTGT GGCCAACATT GAATTTTCAC GGTGATCCAG GCTCTTGTCC TTTCAATACG 
AAGAGACGAG AAAAACTGGA AAAATTAGGG AAAGAAGTAA AAGAAAACAG GGAAGGTATT 
CCGGCTATCA ACCAAGAGGA GAAAAAAGAA ATGACTCGGC GACCACGAGC AACTGTCCGC 
ACTCGAAGAA CAGAACAATT AGCTTCCGGT TATTGTGAAA TTTGCCGTAT TAGATATAGA 
GATCTGGCCA AACATGTGCA AAGCGATCAG CATCTTAGCT TTATTCAAAA TGATGATAAT 
TTCTTGTCTT TGGATAAATT GATCAATGCA GGAGCCAATG TAGAAGCGTT TTTAAAACTA 
AACAGAGGAA AAAATATAGA AAAAGATTGC AATTTGTTTT CCAATGGGGA TCGTAATCTT 
CATGACACAG TATTGCCAGA AGGGAAGGTT AATAGAAATG CAAAAAGTTT AGGGGATTTT 
GACGTTGGAG ACATAAAGAT GGTACAACGT AATGGCGCAC GTCGGAATCT CAGTTTAAGA 
TTAAACTCCT CTCATAATTT ACGTACACGT ACAAGACACG AAAGCGGCCA TTTATTAAGA 
TCAAAAGGTA GTCCCTGGCA TGAAGCCGAT AAAACGGAGA AGTTCTACGA CGAATTCGAA 
GGTTTTACTA TCAAAAAGCG AGCGAAAGGG ACAATTTGGA TCGAAGAAGA TGCACCAGGA 
GACAAATATA TAGAAGAACA TGAATTAAAA GAATCTAAGC AAAATGAATA CAAAATTAAA 
ACATTTTCGG CGGAATTAGA AGAAAAATGT TGTAATGAGA AAAATTGTGA AACATATAAC 
AAACTTAAAG ATACTAACAA TCAGGACATA CAAAGGACAA TAAATTGTAA TAACGAAGAA 
AATAGTATTG GAAATGACCC TGAACGCATC AAACAAGAAC AAACTCATCC AAAGAGTAAA 
AATCATGATC AAATTAATGG AAACATAAAA AATGGTTGTA ATGAAAATCT TTTAAGTACA 
AATGATAACA GTCGTATTAA ACCTTGTAAA GTTTCGCCTG GTAACGAAAC TTTAAAAGAA 
TTGACTTGCA AATTACAGTT AGAAAATACT CCATCAAGTG CAAAAAGGTT ATCAGAGCAC 
AATGATATCA ATACAGGCAC AATCTGTAAT GGTCATTCTG TAAGGGATGA ACAGAAAATT 
AATAGAACAC TGAATAATAC AGAAAAGAAC GAAGTAGCAA AAGAAGAGAA ATCAAAATGT 
TGCAAGTCAA ATAGAAGAGG TAGTCGGAGC GTCAGAGGAC GGCACAGGTT GTCCGTTGAA 
GAACGTTTGA TTGAAGATAA TCGTGCATAT TATAAAGTGG AAGTGTTAGG AAGTAAGTTA 
AGATCAAGTG TATTGTCAAA CAATAATTCT CAATGTACAG TTCAAAAGGA TGGGGATAGT 
GAAGAAAAGA AGGAAGTGCC ATCTAGTGAG AAACCAGTAG TTGTACGATT TAAACGTGTA 
AGAAAGTCGG AACTATCATT ACTAAGCGAT GAAGCGGAGT CTTTTATGTT TAGGGAACTT 
AAACGAGATG ATTCAAGTGA AATAAGCGAT GACGAACAGA GTAGCGTATT ACCAAGGGAT 
ACTGAAAGTG AGTGCAACGA TAGCGGCAAT TCTATCTGTC CTAGTTCATT CTTAACTTCT 
AGTTCACCTG CAAAATCGGA AACTACCGAA GACGATTCTC AAGACTCTTT AAGTTTGGGA 
CGAGCTAGAA AAAAAAGACG GACACAAGCA GAAGCGCTGA TCAAAGATAA TGTTGATTAT 
TACAAATTCG AAACTCCTGG GAGCAGATTA AGGTATCAGG CGCCTCTAAC TGGTATTAAA 
GAAAATCAGG AAGAAATAGA AGGTAGTATT GTCGAGAAAG GTATAGAAAT AGCAAAGGTG 
TATCCGTCCA AACCCTCACC AGAAGTTGAA AAAATGCAAT TTTCTTTTGA AGCCGTACCA 
AAGTCTGAAC CATGGTATCA AACGTATCAG CGGCAAGATG AAGGAGCAGA ATTTTGGCAC 
TATTTTAGCG AAGGGGACTC ACAAAAGCCA TTTCTTTTAC CATATGAAAT TGAAAATTTT 
CACGAAATTT TGATAAAAAA CCAAAATAGA GCGGAAAATA AAAGAAAAGG TCGCGGACGG 
AGTATAGGTT GTATTGGACG ATCTCCTAGG AAAAGTCCTC GTTGTCATGC TTCCACATTA 
GCTATTATGT CAACAATTAT AAGAAAAAGA GAACAACAGC AACAACAATC TTCGAATTTG 
TACACAATAG ATGAATGCCA ATCCATCAGG TCACATGCAA ATACTCCCAG ACCTGATCAG 
AAAACTGAGT TGAAATCTGA TGTGGATGAA GATCTAAAAG AAATGGTAAA AAGTATCGAC 
GAGATGCTTA ATAACGCAAA CGATAGTTTC GAGTTGATTG AATCGTTCGA GCCTGATACA 
ACACAGATGG AATTGAATTT TTATGAACCA AATATCCCAA GAGGACCACC TCCAAACTTG 
CTTGAATTAT TGGACAGTTG TCACGATATC GTAAACTGTT TAGAAAATTC ATCATGTGCA 
AGTTCCGAGT GTGGTGAGGG AAGTGTCGAG TCACCTTTAA AACGAAGGAA GAAGAGGAAA 
AATAGAACCG GATGGCCTGG AATTAAAATG AAGAGAAGAC TCCAAAGCAA ACCTCTGGCA 
GACATAAACT GTGACAGGGA AAACCTAGTG GAGAAAAATA TAGGGCAAAG CGAGAAGGAT 
TGTAATGTGC AGGTCACGAA TATGTCTAAC AGATTAACGG AAGAAGGTAA TATTAAAATA 
GCTACATCCG TTACGAGTTC CATCACACAA GATGAACAGT CATTAGGCTT CGGCCAGAGG 
AAGGACGACG GACGTTTATT CGAAGTGTAT ACCTCGAATA TTTCTTCTAA CACGCGTCAC 
GACGAAAACG AGAAGAAAGA CGTTCACTTA CATCTGATAT ATGCAACGAG ACTGACAGGA 
ATTTCATATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012241350.1
CDS	254..3985
Translation	MVSPSKIQHQEHKQPSRENKLNKHSHVHTKKELKSLEGKSFYLDIKNHATTTKIEAKIKDLGGVIELFLIRSVDLVISDRVDKTGYINFEKHKWGCASGGSGGPPSLRSLETPTPMPTPPTSFFSPECPLSNTTNLRGQTTQRSKSRVEAMLERALIQPQQCSVDPLYNAQNWGIPIWAIDKLQSWLDKIYSSVKDTNHLKQLRHSHQQSSTKDIKVRHLKGPYLKFESFQRNTRPVFVELPVWPTLNFHGDPGSCPFNTKRREKLEKLGKEVKENREGIPAINQEEKKEMTRRPRATVRTRRTEQLASGYCEICRIRYRDLAKHVQSDQHLSFIQNDDNFLSLDKLINAGANVEAFLKLNRGKNIEKDCNLFSNGDRNLHDTVLPEGKVNRNAKSLGDFDVGDIKMVQRNGARRNLSLRLNSSHNLRTRTRHESGHLLRSKGSPWHEADKTEKFYDEFEGFTIKKRAKGTIWIEEDAPGDKYIEEHELKESKQNEYKIKTFSAELEEKCCNEKNCETYNKLKDTNNQDIQRTINCNNEENSIGNDPERIKQEQTHPKSKNHDQINGNIKNGCNENLLSTNDNSRIKPCKVSPGNETLKELTCKLQLENTPSSAKRLSEHNDINTGTICNGHSVRDEQKINRTLNNTEKNEVAKEEKSKCCKSNRRGSRSVRGRHRLSVEERLIEDNRAYYKVEVLGSKLRSSVLSNNNSQCTVQKDGDSEEKKEVPSSEKPVVVRFKRVRKSELSLLSDEAESFMFRELKRDDSSEISDDEQSSVLPRDTESECNDSGNSICPSSFLTSSSPAKSETTEDDSQDSLSLGRARKKRRTQAEALIKDNVDYYKFETPGSRLRYQAPLTGIKENQEEIEGSIVEKGIEIAKVYPSKPSPEVEKMQFSFEAVPKSEPWYQTYQRQDEGAEFWHYFSEGDSQKPFLLPYEIENFHEILIKNQNRAENKRKGRGRSIGCIGRSPRKSPRCHASTLAIMSTIIRKREQQQQQSSNLYTIDECQSIRSHANTPRPDQKTELKSDVDEDLKEMVKSIDEMLNNANDSFELIESFEPDTTQMELNFYEPNIPRGPPPNLLELLDSCHDIVNCLENSSCASSECGEGSVESPLKRRKKRKNRTGWPGIKMKRRLQSKPLADINCDRENLVEKNIGQSEKDCNVQVTNMSNRLTEEGNIKIATSVTSSITQDEQSLGFGQRKDDGRLFEVYTSNISSNTRHDENEKKDVHLHLIYATRLTGISY

Target ORF information:

RefSeq Version	XM_012385927.2
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens uncharacterized LOC100745898 (LOC100745898), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012385927.2

ORF Insert Sequence:

ATGGTATCGC CATCCAAAAT TCAACATCAG GAGCATAAGC AGCCATCAAG AGAAAATAAA 
TTAAACAAAC ACTCACATGT TCATACAAAG AAGGAATTGA AATCATTGGA AGGAAAGTCG 
TTTTACTTGG ATATCAAGAA TCATGCTACT ACAACTAAAA TAGAAGCTAA GATTAAAGAT 
TTGGGAGGAG TAATAGAATT ATTTTTAATA CGGAGTGTAG ACCTGGTGAT TAGTGACAGA 
GTAGACAAGA CTGGTTACAT AAATTTTGAA AAACATAAAT GGGGTTGTGC CAGTGGAGGC 
AGCGGGGGAC CTCCCTCGCT GAGGAGTTTA GAAACTCCAA CCCCCATGCC AACTCCTCCA 
ACATCATTTT TTAGTCCTGA ATGCCCCTTG TCTAATACAA CCAATCTACG GGGTCAAACT 
ACCCAAAGAT CTAAATCGCG GGTGGAGGCA ATGTTGGAGC GTGCTCTGAT ACAGCCACAA 
CAGTGTTCCG TGGATCCACT CTACAACGCT CAAAATTGGG GAATTCCTAT TTGGGCAATT 
GATAAACTTC AGTCTTGGCT TGACAAAATC TACTCATCTG TTAAAGACAC GAATCATTTG 
AAACAATTAA GACACTCACA TCAACAGAGT TCAACTAAAG ATATAAAGGT CAGACACCTC 
AAGGGTCCTT ATTTGAAATT TGAATCCTTT CAGAGGAATA CTAGACCTGT ATTTGTTGAA 
CTACCTGTGT GGCCAACATT GAATTTTCAC GGTGATCCAG GCTCTTGTCC TTTCAATACG 
AAGAGACGAG AAAAACTGGA AAAATTAGGG AAAGAAGTAA AAGAAAACAG GGAAGGTATT 
CCGGCTATCA ACCAAGAGGA GAAAAAAGAA ATGACTCGGC GACCACGAGC AACTGTCCGC 
ACTCGAAGAA CAGAACAATT AGCTTCCGGT TATTGTGAAA TTTGCCGTAT TAGATATAGA 
GATCTGGCCA AACATGTGCA AAGCGATCAG CATCTTAGCT TTATTCAAAA TGATGATAAT 
TTCTTGTCTT TGGATAAATT GATCAATGCA GGAGCCAATG TAGAAGCGTT TTTAAAACTA 
AACAGAGGAA AAAATATAGA AAAAGATTGC AATTTGTTTT CCAATGGGGA TCGTAATCTT 
CATGACACAG TATTGCCAGA AGGGAAGGTT AATAGAAATG CAAAAAGTTT AGGGGATTTT 
GACGTTGGAG ACATAAAGAT GGTACAACGT AATGGCGCAC GTCGGAATCT CAGTTTAAGA 
TTAAACTCCT CTCATAATTT ACGTACACGT ACAAGACACG AAAGCGGCCA TTTATTAAGA 
TCAAAAGGTA GTCCCTGGCA TGAAGCCGAT AAAACGGAGA AGTTCTACGA CGAATTCGAA 
GGTTTTACTA TCAAAAAGCG AGCGAAAGGG ACAATTTGGA TCGAAGAAGA TGCACCAGGA 
GACAAATATA TAGAAGAACA TGAATTAAAA GAATCTAAGC AAAATGAATA CAAAATTAAA 
ACATTTTCGG CGGAATTAGA AGAAAAATGT TGTAATGAGA AAAATTGTGA AACATATAAC 
AAACTTAAAG ATACTAACAA TCAGGACATA CAAAGGACAA TAAATTGTAA TAACGAAGAA 
AATAGTATTG GAAATGACCC TGAACGCATC AAACAAGAAC AAACTCATCC AAAGAGTAAA 
AATCATGATC AAATTAATGG AAACATAAAA AATGGTTGTA ATGAAAATCT TTTAAGTACA 
AATGATAACA GTCGTATTAA ACCTTGTAAA GTTTCGCCTG GTAACGAAAC TTTAAAAGAA 
TTGACTTGCA AATTACAGTT AGAAAATACT CCATCAAGTG CAAAAAGGTT ATCAGAGCAC 
AATGATATCA ATACAGGCAC AATCTGTAAT GGTCATTCTG TAAGGGATGA ACAGAAAATT 
AATAGAACAC TGAATAATAC AGAAAAGAAC GAAGTAGCAA AAGAAGAGAA ATCAAAATGT 
TGCAAGTCAA ATAGAAGAGG TAGTCGGAGC GTCAGAGGAC GGCACAGGTT GTCCGTTGAA 
GAACGTTTGA TTGAAGATAA TCGTGCATAT TATAAAGTGG AAGTGTTAGG AAGTAAGTTA 
AGATCAAGTG TATTGTCAAA CAATAATTCT CAATGTACAG TTCAAAAGGA TGGGGATAGT 
GAAGAAAAGA AGGAAGTGCC ATCTAGTGAG AAACCAGTAG TTGTACGATT TAAACGTGTA 
AGAAAGTCGG AACTATCATT ACTAAGCGAT GAAGCGGAGT CTTTTATGTT TAGGGAACTT 
AAACGAGATG ATTCAAGTGA AATAAGCGAT GACGAACAGA GTAGCGTATT ACCAAGGGAT 
ACTGAAAGTG AGTGCAACGA TAGCGGCAAT TCTATCTGTC CTAGTTCATT CTTAACTTCT 
AGTTCACCTG CAAAATCGGA AACTACCGAA GACGATTCTC AAGACTCTTT AAGTTTGGGA 
CGAGCTAGAA AAAAAAGACG GACACAAGCA GAAGCGCTGA TCAAAGATAA TGTTGATTAT 
TACAAATTCG AAACTCCTGG GAGCAGATTA AGGTATCAGG CGCCTCTAAC TGGTATTAAA 
GAAAATCAGG AAGAAATAGA AGGTAGTATT GTCGAGAAAG GTATAGAAAT AGCAAAGGTG 
TATCCGTCCA AACCCTCACC AGAAGTTGAA AAAATGCAAT TTTCTTTTGA AGCCGTACCA 
AAGTCTGAAC CATGGTATCA AACGTATCAG CGGCAAGATG AAGGAGCAGA ATTTTGGCAC 
TATTTTAGCG AAGGGGACTC ACAAAAGCCA TTTCTTTTAC CATATGAAAT TGAAAATTTT 
CACGAAATTT TGATAAAAAA CCAAAATAGA GCGGAAAATA AAAGAAAAGG TCGCGGACGG 
AGTATAGGTT GTATTGGACG ATCTCCTAGG AAAAGTCCTC GTTGTCATGC TTCCACATTA 
GCTATTATGT CAACAATTAT AAGAAAAAGA GAACAACAGC AACAACAATC TTCGAATTTG 
TACACAATAG ATGAATGCCA ATCCATCAGG TCACATGCAA ATACTCCCAG ACCTGATCAG 
AAAACTGAGT TGAAATCTGA TGTGGATGAA GATCTAAAAG AAATGGTAAA AAGTATCGAC 
GAGATGCTTA ATAACGCAAA CGATAGTTTC GAGTTGATTG AATCGTTCGA GCCTGATACA 
ACACAGATGG AATTGAATTT TTATGAACCA AATATCCCAA GAGGACCACC TCCAAACTTG 
CTTGAATTAT TGGACAGTTG TCACGATATC GTAAACTGTT TAGAAAATTC ATCATGTGCA 
AGTTCCGAGT GTGGTGAGGG AAGTGTCGAG TCACCTTTAA AACGAAGGAA GAAGAGGAAA 
AATAGAACCG GATGGCCTGG AATTAAAATG AAGAGAAGAC TCCAAAGCAA ACCTCTGGCA 
GACATAAACT GTGACAGGGA AAACCTAGTG GAGAAAAATA TAGGGCAAAG CGAGAAGGAT 
TGTAATGTGC AGGTCACGAA TATGTCTAAC AGATTAACGG AAGAAGGTAA TATTAAAATA 
GCTACATCCG TTACGAGTTC CATCACACAA GATGAACAGT CATTAGGCTT CGGCCAGAGG 
AAGGACGACG GACGTTTATT CGAAGTGTAT ACCTCGAATA TTTCTTCTAA CACGCGTCAC 
GACGAAAACG AGAAGAAAGA CGTTCACTTA CATCTGATAT ATGCAACGAG ACTGACAGGA 
ATTTCATATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100745898 ( XM_003489654.3 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_003489702.1 Bombus impatiens uncharacterized LOC100745898 (LOC100745898), transcript variant X1, mRNA.

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CloneID	OBo104095
Clone ID Related Accession (Same CDS sequence)	XM_003489654.3 , XM_003489654.2
Accession Version	XM_003489654.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-18
Organism	Bombus impatiens(common eastern bumble bee)
Product	uncharacterized protein LOC100745898 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176780.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 19, 2018 this sequence version replaced XM_003489654.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bombus impatiens Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATCGC CATCCAAAAT TCAACATCAG GAGCATAAGC AGCCATCAAG AGAAAATAAA 
TTAAACAAAC ACTCACATGT TCATACAAAG AAGGAATTGA AATCATTGGA AGGAAAGTCG 
TTTTACTTGG ATATCAAGAA TCATGCTACT ACAACTAAAA TAGAAGCTAA GATTAAAGAT 
TTGGGAGGAG TAATAGAATT ATTTTTAATA CGGAGTGTAG ACCTGGTGAT TAGTGACAGA 
GTAGACAAGA CTGGTTACAT AAATTTTGAA AAACATAAAT GGGGTTGTGC CAGTGGAGGC 
AGCGGGGGAC CTCCCTCGCT GAGGAGTTTA GAAACTCCAA CCCCCATGCC AACTCCTCCA 
ACATCATTTT TTAGTCCTGA ATGCCCCTTG TCTAATACAA CCAATCTACG GGGTCAAACT 
ACCCAAAGAT CTAAATCGCG GGTGGAGGCA ATGTTGGAGC GTGCTCTGAT ACAGCCACAA 
CAGTGTTCCG TGGATCCACT CTACAACGCT CAAAATTGGG GAATTCCTAT TTGGGCAATT 
GATAAACTTC AGTCTTGGCT TGACAAAATC TACTCATCTG TTAAAGACAC GAATCATTTG 
AAACAATTAA GACACTCACA TCAACAGAGT TCAACTAAAG ATATAAAGGT CAGACACCTC 
AAGGGTCCTT ATTTGAAATT TGAATCCTTT CAGAGGAATA CTAGACCTGT ATTTGTTGAA 
CTACCTGTGT GGCCAACATT GAATTTTCAC GGTGATCCAG GCTCTTGTCC TTTCAATACG 
AAGAGACGAG AAAAACTGGA AAAATTAGGG AAAGAAGTAA AAGAAAACAG GGAAGGTATT 
CCGGCTATCA ACCAAGAGGA GAAAAAAGAA ATGACTCGGC GACCACGAGC AACTGTCCGC 
ACTCGAAGAA CAGAACAATT AGCTTCCGGT TATTGTGAAA TTTGCCGTAT TAGATATAGA 
GATCTGGCCA AACATGTGCA AAGCGATCAG CATCTTAGCT TTATTCAAAA TGATGATAAT 
TTCTTGTCTT TGGATAAATT GATCAATGCA GGAGCCAATG TAGAAGCGTT TTTAAAACTA 
AACAGAGGAA AAAATATAGA AAAAGATTGC AATTTGTTTT CCAATGGGGA TCGTAATCTT 
CATGACACAG TATTGCCAGA AGGGAAGGTT AATAGAAATG CAAAAAGTTT AGGGGATTTT 
GACGTTGGAG ACATAAAGAT GGTACAACGT AATGGCGCAC GTCGGAATCT CAGTTTAAGA 
TTAAACTCCT CTCATAATTT ACGTACACGT ACAAGACACG AAAGCGGCCA TTTATTAAGA 
TCAAAAGGTA GTCCCTGGCA TGAAGCCGAT AAAACGGAGA AGTTCTACGA CGAATTCGAA 
GGTTTTACTA TCAAAAAGCG AGCGAAAGGG ACAATTTGGA TCGAAGAAGA TGCACCAGGA 
GACAAATATA TAGAAGAACA TGAATTAAAA GAATCTAAGC AAAATGAATA CAAAATTAAA 
ACATTTTCGG CGGAATTAGA AGAAAAATGT TGTAATGAGA AAAATTGTGA AACATATAAC 
AAACTTAAAG ATACTAACAA TCAGGACATA CAAAGGACAA TAAATTGTAA TAACGAAGAA 
AATAGTATTG GAAATGACCC TGAACGCATC AAACAAGAAC AAACTCATCC AAAGAGTAAA 
AATCATGATC AAATTAATGG AAACATAAAA AATGGTTGTA ATGAAAATCT TTTAAGTACA 
AATGATAACA GTCGTATTAA ACCTTGTAAA GTTTCGCCTG GTAACGAAAC TTTAAAAGAA 
TTGACTTGCA AATTACAGTT AGAAAATACT CCATCAAGTG CAAAAAGGTT ATCAGAGCAC 
AATGATATCA ATACAGGCAC AATCTGTAAT GGTCATTCTG TAAGGGATGA ACAGAAAATT 
AATAGAACAC TGAATAATAC AGAAAAGAAC GAAGTAGCAA AAGAAGAGAA ATCAAAATGT 
TGCAAGTCAA ATAGAAGAGG TAGTCGGAGC GTCAGAGGAC GGCACAGGTT GTCCGTTGAA 
GAACGTTTGA TTGAAGATAA TCGTGCATAT TATAAAGTGG AAGTGTTAGG AAGTAAGTTA 
AGATCAAGTG TATTGTCAAA CAATAATTCT CAATGTACAG TTCAAAAGGA TGGGGATAGT 
GAAGAAAAGA AGGAAGTGCC ATCTAGTGAG AAACCAGTAG TTGTACGATT TAAACGTGTA 
AGAAAGTCGG AACTATCATT ACTAAGCGAT GAAGCGGAGT CTTTTATGTT TAGGGAACTT 
AAACGAGATG ATTCAAGTGA AATAAGCGAT GACGAACAGA GTAGCGTATT ACCAAGGGAT 
ACTGAAAGTG AGTGCAACGA TAGCGGCAAT TCTATCTGTC CTAGTTCATT CTTAACTTCT 
AGTTCACCTG CAAAATCGGA AACTACCGAA GACGATTCTC AAGACTCTTT AAGTTTGGGA 
CGAGCTAGAA AAAAAAGACG GACACAAGCA GAAGCGCTGA TCAAAGATAA TGTTGATTAT 
TACAAATTCG AAACTCCTGG GAGCAGATTA AGGTATCAGG CGCCTCTAAC TGGTATTAAA 
GAAAATCAGG AAGAAATAGA AGGTAGTATT GTCGAGAAAG GTATAGAAAT AGCAAAGGTG 
TATCCGTCCA AACCCTCACC AGAAGTTGAA AAAATGCAAT TTTCTTTTGA AGCCGTACCA 
AAGTCTGAAC CATGGTATCA AACGTATCAG CGGCAAGATG AAGGAGCAGA ATTTTGGCAC 
TATTTTAGCG AAGGGGACTC ACAAAAGCCA TTTCTTTTAC CATATGAAAT TGAAAATTTT 
CACGAAATTT TGATAAAAAA CCAAAATAGA GCGGAAAATA AAAGAAAAGG TCGCGGACGG 
AGTATAGGTT GTATTGGACG ATCTCCTAGG AAAAGTCCTC GTTGTCATGC TTCCACATTA 
GCTATTATGT CAACAATTAT AAGAAAAAGA GAACAACAGC AACAACAATC TTCGAATTTG 
TACACAATAG ATGAATGCCA ATCCATCAGG TCACATGCAA ATACTCCCAG ACCTGATCAG 
AAAACTGAGT TGAAATCTGA TGTGGATGAA GATCTAAAAG AAATGGTAAA AAGTATCGAC 
GAGATGCTTA ATAACGCAAA CGATAGTTTC GAGTTGATTG AATCGTTCGA GCCTGATACA 
ACACAGATGG AATTGAATTT TTATGAACCA AATATCCCAA GAGGACCACC TCCAAACTTG 
CTTGAATTAT TGGACAGTTG TCACGATATC GTAAACTGTT TAGAAAATTC ATCATGTGCA 
AGTTCCGAGT GTGGTGAGGG AAGTGTCGAG TCACCTTTAA AACGAAGGAA GAAGAGGAAA 
AATAGAACCG GATGGCCTGG AATTAAAATG AAGAGAAGAC TCCAAAGCAA ACCTCTGGCA 
GACATAAACT GTGACAGGGA AAACCTAGTG GAGAAAAATA TAGGGCAAAG CGAGAAGGAT 
TGTAATGTGC AGGTCACGAA TATGTCTAAC AGATTAACGG AAGAAGGTAA TATTAAAATA 
GCTACATCCG TTACGAGTTC CATCACACAA GATGAACAGT CATTAGGCTT CGGCCAGAGG 
AAGGACGACG GACGTTTATT CGAAGTGTAT ACCTCGAATA TTTCTTCTAA CACGCGTCAC 
GACGAAAACG AGAAGAAAGA CGTAAGTATA GCGGTTCCTG ATAATTCCAT GTTACATACA 
ACAAATGCTG AATCCTGCAC AGGTTCACTT ACATCTGATA TATGCAACGA GACTGACAGG 
AATTTCATAT TGAACAAAGA CTATGGATTC AGAAAAAATT TGTCGAAGAT CGAAGAAATG 
TCAGAAAATG ATCACGGAAA CGATGAAAAT CACGAGAACG TGTTTCGGAA AGATGTTCAT 
TCTATATCGG AACGTCGTTT TATCTCGAAC GCAACCATCA AGAAACGACA ACATGACAAC 
ACATCTTCTG AAACTTGCGA ATCTCGTGTA GAACTCGAAA ATCATGAGAT ATTGTGTAGA 
AAGGACGCCG AGACCAAAAT TGTTTCCAGG AAACATCATA ATACTAACGG ATTACCGAGA 
AAGAGGCAAC AGAAGACTCA TTCTGAAAAC TCTGACTCTG AAATTGAACA GCATCATCGC 
CTCCATCATC ATCACCATCA CCACCTTCAC CATAGTAATG TATATAAAAG GAACAGAGTA 
ACTTCGCGAA AACGAATTTG CGCGAAGAAA CGGACGAGAA AAACTGACAT ATCTTCAGAT 
ACAGTGTTCG AAGATGAGAA TTGTAAAAAG GATTCCTATT TAAGTATTAC ATGTAGAAAG 
CAACAAACTA TAAAAGGTAC AAAACGACGA GCCGATACCA TGTCATCGGA AACACAAAAT 
TCTGAAGTCG ATTGTGCATT ATCGGGACAT GTCAGTCCTA CGATTATAAC TACTTCAGAA 
CTTGAACAAA GACGTTCGAG TATTGAGTTT CAACCAGTTG TTAGAATGAT GAAGATTGAC 
GATCAAGTTG AAATGGACCA CAGTATTCTC TCGGTAACGA TTGCAAGCAA CAGACGGTTA 
AGAAGTTCCA GTTCTCCGAG ATCGAATACT CAACCGCCGA AAAAACGTTT AAAGACCAAT 
CGTGGTCAAT TCGGAAGGTG GTTAAAAAGT AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003489702.1
CDS	253..4908
Translation	MVSPSKIQHQEHKQPSRENKLNKHSHVHTKKELKSLEGKSFYLDIKNHATTTKIEAKIKDLGGVIELFLIRSVDLVISDRVDKTGYINFEKHKWGCASGGSGGPPSLRSLETPTPMPTPPTSFFSPECPLSNTTNLRGQTTQRSKSRVEAMLERALIQPQQCSVDPLYNAQNWGIPIWAIDKLQSWLDKIYSSVKDTNHLKQLRHSHQQSSTKDIKVRHLKGPYLKFESFQRNTRPVFVELPVWPTLNFHGDPGSCPFNTKRREKLEKLGKEVKENREGIPAINQEEKKEMTRRPRATVRTRRTEQLASGYCEICRIRYRDLAKHVQSDQHLSFIQNDDNFLSLDKLINAGANVEAFLKLNRGKNIEKDCNLFSNGDRNLHDTVLPEGKVNRNAKSLGDFDVGDIKMVQRNGARRNLSLRLNSSHNLRTRTRHESGHLLRSKGSPWHEADKTEKFYDEFEGFTIKKRAKGTIWIEEDAPGDKYIEEHELKESKQNEYKIKTFSAELEEKCCNEKNCETYNKLKDTNNQDIQRTINCNNEENSIGNDPERIKQEQTHPKSKNHDQINGNIKNGCNENLLSTNDNSRIKPCKVSPGNETLKELTCKLQLENTPSSAKRLSEHNDINTGTICNGHSVRDEQKINRTLNNTEKNEVAKEEKSKCCKSNRRGSRSVRGRHRLSVEERLIEDNRAYYKVEVLGSKLRSSVLSNNNSQCTVQKDGDSEEKKEVPSSEKPVVVRFKRVRKSELSLLSDEAESFMFRELKRDDSSEISDDEQSSVLPRDTESECNDSGNSICPSSFLTSSSPAKSETTEDDSQDSLSLGRARKKRRTQAEALIKDNVDYYKFETPGSRLRYQAPLTGIKENQEEIEGSIVEKGIEIAKVYPSKPSPEVEKMQFSFEAVPKSEPWYQTYQRQDEGAEFWHYFSEGDSQKPFLLPYEIENFHEILIKNQNRAENKRKGRGRSIGCIGRSPRKSPRCHASTLAIMSTIIRKREQQQQQSSNLYTIDECQSIRSHANTPRPDQKTELKSDVDEDLKEMVKSIDEMLNNANDSFELIESFEPDTTQMELNFYEPNIPRGPPPNLLELLDSCHDIVNCLENSSCASSECGEGSVESPLKRRKKRKNRTGWPGIKMKRRLQSKPLADINCDRENLVEKNIGQSEKDCNVQVTNMSNRLTEEGNIKIATSVTSSITQDEQSLGFGQRKDDGRLFEVYTSNISSNTRHDENEKKDVSIAVPDNSMLHTTNAESCTGSLTSDICNETDRNFILNKDYGFRKNLSKIEEMSENDHGNDENHENVFRKDVHSISERRFISNATIKKRQHDNTSSETCESRVELENHEILCRKDAETKIVSRKHHNTNGLPRKRQQKTHSENSDSEIEQHHRLHHHHHHHLHHSNVYKRNRVTSRKRICAKKRTRKTDISSDTVFEDENCKKDSYLSITCRKQQTIKGTKRRADTMSSETQNSEVDCALSGHVSPTIITTSELEQRRSSIEFQPVVRMMKIDDQVEMDHSILSVTIASNRRLRSSSSPRSNTQPPKKRLKTNRGQFGRWLKSS

Target ORF information:

RefSeq Version	XM_003489654.3
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens uncharacterized LOC100745898 (LOC100745898), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003489654.3

ORF Insert Sequence:

ATGGTATCGC CATCCAAAAT TCAACATCAG GAGCATAAGC AGCCATCAAG AGAAAATAAA 
TTAAACAAAC ACTCACATGT TCATACAAAG AAGGAATTGA AATCATTGGA AGGAAAGTCG 
TTTTACTTGG ATATCAAGAA TCATGCTACT ACAACTAAAA TAGAAGCTAA GATTAAAGAT 
TTGGGAGGAG TAATAGAATT ATTTTTAATA CGGAGTGTAG ACCTGGTGAT TAGTGACAGA 
GTAGACAAGA CTGGTTACAT AAATTTTGAA AAACATAAAT GGGGTTGTGC CAGTGGAGGC 
AGCGGGGGAC CTCCCTCGCT GAGGAGTTTA GAAACTCCAA CCCCCATGCC AACTCCTCCA 
ACATCATTTT TTAGTCCTGA ATGCCCCTTG TCTAATACAA CCAATCTACG GGGTCAAACT 
ACCCAAAGAT CTAAATCGCG GGTGGAGGCA ATGTTGGAGC GTGCTCTGAT ACAGCCACAA 
CAGTGTTCCG TGGATCCACT CTACAACGCT CAAAATTGGG GAATTCCTAT TTGGGCAATT 
GATAAACTTC AGTCTTGGCT TGACAAAATC TACTCATCTG TTAAAGACAC GAATCATTTG 
AAACAATTAA GACACTCACA TCAACAGAGT TCAACTAAAG ATATAAAGGT CAGACACCTC 
AAGGGTCCTT ATTTGAAATT TGAATCCTTT CAGAGGAATA CTAGACCTGT ATTTGTTGAA 
CTACCTGTGT GGCCAACATT GAATTTTCAC GGTGATCCAG GCTCTTGTCC TTTCAATACG 
AAGAGACGAG AAAAACTGGA AAAATTAGGG AAAGAAGTAA AAGAAAACAG GGAAGGTATT 
CCGGCTATCA ACCAAGAGGA GAAAAAAGAA ATGACTCGGC GACCACGAGC AACTGTCCGC 
ACTCGAAGAA CAGAACAATT AGCTTCCGGT TATTGTGAAA TTTGCCGTAT TAGATATAGA 
GATCTGGCCA AACATGTGCA AAGCGATCAG CATCTTAGCT TTATTCAAAA TGATGATAAT 
TTCTTGTCTT TGGATAAATT GATCAATGCA GGAGCCAATG TAGAAGCGTT TTTAAAACTA 
AACAGAGGAA AAAATATAGA AAAAGATTGC AATTTGTTTT CCAATGGGGA TCGTAATCTT 
CATGACACAG TATTGCCAGA AGGGAAGGTT AATAGAAATG CAAAAAGTTT AGGGGATTTT 
GACGTTGGAG ACATAAAGAT GGTACAACGT AATGGCGCAC GTCGGAATCT CAGTTTAAGA 
TTAAACTCCT CTCATAATTT ACGTACACGT ACAAGACACG AAAGCGGCCA TTTATTAAGA 
TCAAAAGGTA GTCCCTGGCA TGAAGCCGAT AAAACGGAGA AGTTCTACGA CGAATTCGAA 
GGTTTTACTA TCAAAAAGCG AGCGAAAGGG ACAATTTGGA TCGAAGAAGA TGCACCAGGA 
GACAAATATA TAGAAGAACA TGAATTAAAA GAATCTAAGC AAAATGAATA CAAAATTAAA 
ACATTTTCGG CGGAATTAGA AGAAAAATGT TGTAATGAGA AAAATTGTGA AACATATAAC 
AAACTTAAAG ATACTAACAA TCAGGACATA CAAAGGACAA TAAATTGTAA TAACGAAGAA 
AATAGTATTG GAAATGACCC TGAACGCATC AAACAAGAAC AAACTCATCC AAAGAGTAAA 
AATCATGATC AAATTAATGG AAACATAAAA AATGGTTGTA ATGAAAATCT TTTAAGTACA 
AATGATAACA GTCGTATTAA ACCTTGTAAA GTTTCGCCTG GTAACGAAAC TTTAAAAGAA 
TTGACTTGCA AATTACAGTT AGAAAATACT CCATCAAGTG CAAAAAGGTT ATCAGAGCAC 
AATGATATCA ATACAGGCAC AATCTGTAAT GGTCATTCTG TAAGGGATGA ACAGAAAATT 
AATAGAACAC TGAATAATAC AGAAAAGAAC GAAGTAGCAA AAGAAGAGAA ATCAAAATGT 
TGCAAGTCAA ATAGAAGAGG TAGTCGGAGC GTCAGAGGAC GGCACAGGTT GTCCGTTGAA 
GAACGTTTGA TTGAAGATAA TCGTGCATAT TATAAAGTGG AAGTGTTAGG AAGTAAGTTA 
AGATCAAGTG TATTGTCAAA CAATAATTCT CAATGTACAG TTCAAAAGGA TGGGGATAGT 
GAAGAAAAGA AGGAAGTGCC ATCTAGTGAG AAACCAGTAG TTGTACGATT TAAACGTGTA 
AGAAAGTCGG AACTATCATT ACTAAGCGAT GAAGCGGAGT CTTTTATGTT TAGGGAACTT 
AAACGAGATG ATTCAAGTGA AATAAGCGAT GACGAACAGA GTAGCGTATT ACCAAGGGAT 
ACTGAAAGTG AGTGCAACGA TAGCGGCAAT TCTATCTGTC CTAGTTCATT CTTAACTTCT 
AGTTCACCTG CAAAATCGGA AACTACCGAA GACGATTCTC AAGACTCTTT AAGTTTGGGA 
CGAGCTAGAA AAAAAAGACG GACACAAGCA GAAGCGCTGA TCAAAGATAA TGTTGATTAT 
TACAAATTCG AAACTCCTGG GAGCAGATTA AGGTATCAGG CGCCTCTAAC TGGTATTAAA 
GAAAATCAGG AAGAAATAGA AGGTAGTATT GTCGAGAAAG GTATAGAAAT AGCAAAGGTG 
TATCCGTCCA AACCCTCACC AGAAGTTGAA AAAATGCAAT TTTCTTTTGA AGCCGTACCA 
AAGTCTGAAC CATGGTATCA AACGTATCAG CGGCAAGATG AAGGAGCAGA ATTTTGGCAC 
TATTTTAGCG AAGGGGACTC ACAAAAGCCA TTTCTTTTAC CATATGAAAT TGAAAATTTT 
CACGAAATTT TGATAAAAAA CCAAAATAGA GCGGAAAATA AAAGAAAAGG TCGCGGACGG 
AGTATAGGTT GTATTGGACG ATCTCCTAGG AAAAGTCCTC GTTGTCATGC TTCCACATTA 
GCTATTATGT CAACAATTAT AAGAAAAAGA GAACAACAGC AACAACAATC TTCGAATTTG 
TACACAATAG ATGAATGCCA ATCCATCAGG TCACATGCAA ATACTCCCAG ACCTGATCAG 
AAAACTGAGT TGAAATCTGA TGTGGATGAA GATCTAAAAG AAATGGTAAA AAGTATCGAC 
GAGATGCTTA ATAACGCAAA CGATAGTTTC GAGTTGATTG AATCGTTCGA GCCTGATACA 
ACACAGATGG AATTGAATTT TTATGAACCA AATATCCCAA GAGGACCACC TCCAAACTTG 
CTTGAATTAT TGGACAGTTG TCACGATATC GTAAACTGTT TAGAAAATTC ATCATGTGCA 
AGTTCCGAGT GTGGTGAGGG AAGTGTCGAG TCACCTTTAA AACGAAGGAA GAAGAGGAAA 
AATAGAACCG GATGGCCTGG AATTAAAATG AAGAGAAGAC TCCAAAGCAA ACCTCTGGCA 
GACATAAACT GTGACAGGGA AAACCTAGTG GAGAAAAATA TAGGGCAAAG CGAGAAGGAT 
TGTAATGTGC AGGTCACGAA TATGTCTAAC AGATTAACGG AAGAAGGTAA TATTAAAATA 
GCTACATCCG TTACGAGTTC CATCACACAA GATGAACAGT CATTAGGCTT CGGCCAGAGG 
AAGGACGACG GACGTTTATT CGAAGTGTAT ACCTCGAATA TTTCTTCTAA CACGCGTCAC 
GACGAAAACG AGAAGAAAGA CGTAAGTATA GCGGTTCCTG ATAATTCCAT GTTACATACA 
ACAAATGCTG AATCCTGCAC AGGTTCACTT ACATCTGATA TATGCAACGA GACTGACAGG 
AATTTCATAT TGAACAAAGA CTATGGATTC AGAAAAAATT TGTCGAAGAT CGAAGAAATG 
TCAGAAAATG ATCACGGAAA CGATGAAAAT CACGAGAACG TGTTTCGGAA AGATGTTCAT 
TCTATATCGG AACGTCGTTT TATCTCGAAC GCAACCATCA AGAAACGACA ACATGACAAC 
ACATCTTCTG AAACTTGCGA ATCTCGTGTA GAACTCGAAA ATCATGAGAT ATTGTGTAGA 
AAGGACGCCG AGACCAAAAT TGTTTCCAGG AAACATCATA ATACTAACGG ATTACCGAGA 
AAGAGGCAAC AGAAGACTCA TTCTGAAAAC TCTGACTCTG AAATTGAACA GCATCATCGC 
CTCCATCATC ATCACCATCA CCACCTTCAC CATAGTAATG TATATAAAAG GAACAGAGTA 
ACTTCGCGAA AACGAATTTG CGCGAAGAAA CGGACGAGAA AAACTGACAT ATCTTCAGAT 
ACAGTGTTCG AAGATGAGAA TTGTAAAAAG GATTCCTATT TAAGTATTAC ATGTAGAAAG 
CAACAAACTA TAAAAGGTAC AAAACGACGA GCCGATACCA TGTCATCGGA AACACAAAAT 
TCTGAAGTCG ATTGTGCATT ATCGGGACAT GTCAGTCCTA CGATTATAAC TACTTCAGAA 
CTTGAACAAA GACGTTCGAG TATTGAGTTT CAACCAGTTG TTAGAATGAT GAAGATTGAC 
GATCAAGTTG AAATGGACCA CAGTATTCTC TCGGTAACGA TTGCAAGCAA CAGACGGTTA 
AGAAGTTCCA GTTCTCCGAG ATCGAATACT CAACCGCCGA AAAAACGTTT AAAGACCAAT 
CGTGGTCAAT TCGGAAGGTG GTTAAAAAGT AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100745898 ( XM_003489654.2 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_003489702.1 Bombus impatiens uncharacterized LOC100745898 (LOC100745898), transcript variant X1, mRNA.

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CloneID	OBo104095
Clone ID Related Accession (Same CDS sequence)	XM_003489654.3 , XM_003489654.2
Accession Version	XM_003489654.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-27
Organism	Bombus impatiens(common eastern bumble bee)
Product	uncharacterized protein LOC100745898 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176780.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2015 this sequence version replaced XM_003489654.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombus impatiens Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATCGC CATCCAAAAT TCAACATCAG GAGCATAAGC AGCCATCAAG AGAAAATAAA 
TTAAACAAAC ACTCACATGT TCATACAAAG AAGGAATTGA AATCATTGGA AGGAAAGTCG 
TTTTACTTGG ATATCAAGAA TCATGCTACT ACAACTAAAA TAGAAGCTAA GATTAAAGAT 
TTGGGAGGAG TAATAGAATT ATTTTTAATA CGGAGTGTAG ACCTGGTGAT TAGTGACAGA 
GTAGACAAGA CTGGTTACAT AAATTTTGAA AAACATAAAT GGGGTTGTGC CAGTGGAGGC 
AGCGGGGGAC CTCCCTCGCT GAGGAGTTTA GAAACTCCAA CCCCCATGCC AACTCCTCCA 
ACATCATTTT TTAGTCCTGA ATGCCCCTTG TCTAATACAA CCAATCTACG GGGTCAAACT 
ACCCAAAGAT CTAAATCGCG GGTGGAGGCA ATGTTGGAGC GTGCTCTGAT ACAGCCACAA 
CAGTGTTCCG TGGATCCACT CTACAACGCT CAAAATTGGG GAATTCCTAT TTGGGCAATT 
GATAAACTTC AGTCTTGGCT TGACAAAATC TACTCATCTG TTAAAGACAC GAATCATTTG 
AAACAATTAA GACACTCACA TCAACAGAGT TCAACTAAAG ATATAAAGGT CAGACACCTC 
AAGGGTCCTT ATTTGAAATT TGAATCCTTT CAGAGGAATA CTAGACCTGT ATTTGTTGAA 
CTACCTGTGT GGCCAACATT GAATTTTCAC GGTGATCCAG GCTCTTGTCC TTTCAATACG 
AAGAGACGAG AAAAACTGGA AAAATTAGGG AAAGAAGTAA AAGAAAACAG GGAAGGTATT 
CCGGCTATCA ACCAAGAGGA GAAAAAAGAA ATGACTCGGC GACCACGAGC AACTGTCCGC 
ACTCGAAGAA CAGAACAATT AGCTTCCGGT TATTGTGAAA TTTGCCGTAT TAGATATAGA 
GATCTGGCCA AACATGTGCA AAGCGATCAG CATCTTAGCT TTATTCAAAA TGATGATAAT 
TTCTTGTCTT TGGATAAATT GATCAATGCA GGAGCCAATG TAGAAGCGTT TTTAAAACTA 
AACAGAGGAA AAAATATAGA AAAAGATTGC AATTTGTTTT CCAATGGGGA TCGTAATCTT 
CATGACACAG TATTGCCAGA AGGGAAGGTT AATAGAAATG CAAAAAGTTT AGGGGATTTT 
GACGTTGGAG ACATAAAGAT GGTACAACGT AATGGCGCAC GTCGGAATCT CAGTTTAAGA 
TTAAACTCCT CTCATAATTT ACGTACACGT ACAAGACACG AAAGCGGCCA TTTATTAAGA 
TCAAAAGGTA GTCCCTGGCA TGAAGCCGAT AAAACGGAGA AGTTCTACGA CGAATTCGAA 
GGTTTTACTA TCAAAAAGCG AGCGAAAGGG ACAATTTGGA TCGAAGAAGA TGCACCAGGA 
GACAAATATA TAGAAGAACA TGAATTAAAA GAATCTAAGC AAAATGAATA CAAAATTAAA 
ACATTTTCGG CGGAATTAGA AGAAAAATGT TGTAATGAGA AAAATTGTGA AACATATAAC 
AAACTTAAAG ATACTAACAA TCAGGACATA CAAAGGACAA TAAATTGTAA TAACGAAGAA 
AATAGTATTG GAAATGACCC TGAACGCATC AAACAAGAAC AAACTCATCC AAAGAGTAAA 
AATCATGATC AAATTAATGG AAACATAAAA AATGGTTGTA ATGAAAATCT TTTAAGTACA 
AATGATAACA GTCGTATTAA ACCTTGTAAA GTTTCGCCTG GTAACGAAAC TTTAAAAGAA 
TTGACTTGCA AATTACAGTT AGAAAATACT CCATCAAGTG CAAAAAGGTT ATCAGAGCAC 
AATGATATCA ATACAGGCAC AATCTGTAAT GGTCATTCTG TAAGGGATGA ACAGAAAATT 
AATAGAACAC TGAATAATAC AGAAAAGAAC GAAGTAGCAA AAGAAGAGAA ATCAAAATGT 
TGCAAGTCAA ATAGAAGAGG TAGTCGGAGC GTCAGAGGAC GGCACAGGTT GTCCGTTGAA 
GAACGTTTGA TTGAAGATAA TCGTGCATAT TATAAAGTGG AAGTGTTAGG AAGTAAGTTA 
AGATCAAGTG TATTGTCAAA CAATAATTCT CAATGTACAG TTCAAAAGGA TGGGGATAGT 
GAAGAAAAGA AGGAAGTGCC ATCTAGTGAG AAACCAGTAG TTGTACGATT TAAACGTGTA 
AGAAAGTCGG AACTATCATT ACTAAGCGAT GAAGCGGAGT CTTTTATGTT TAGGGAACTT 
AAACGAGATG ATTCAAGTGA AATAAGCGAT GACGAACAGA GTAGCGTATT ACCAAGGGAT 
ACTGAAAGTG AGTGCAACGA TAGCGGCAAT TCTATCTGTC CTAGTTCATT CTTAACTTCT 
AGTTCACCTG CAAAATCGGA AACTACCGAA GACGATTCTC AAGACTCTTT AAGTTTGGGA 
CGAGCTAGAA AAAAAAGACG GACACAAGCA GAAGCGCTGA TCAAAGATAA TGTTGATTAT 
TACAAATTCG AAACTCCTGG GAGCAGATTA AGGTATCAGG CGCCTCTAAC TGGTATTAAA 
GAAAATCAGG AAGAAATAGA AGGTAGTATT GTCGAGAAAG GTATAGAAAT AGCAAAGGTG 
TATCCGTCCA AACCCTCACC AGAAGTTGAA AAAATGCAAT TTTCTTTTGA AGCCGTACCA 
AAGTCTGAAC CATGGTATCA AACGTATCAG CGGCAAGATG AAGGAGCAGA ATTTTGGCAC 
TATTTTAGCG AAGGGGACTC ACAAAAGCCA TTTCTTTTAC CATATGAAAT TGAAAATTTT 
CACGAAATTT TGATAAAAAA CCAAAATAGA GCGGAAAATA AAAGAAAAGG TCGCGGACGG 
AGTATAGGTT GTATTGGACG ATCTCCTAGG AAAAGTCCTC GTTGTCATGC TTCCACATTA 
GCTATTATGT CAACAATTAT AAGAAAAAGA GAACAACAGC AACAACAATC TTCGAATTTG 
TACACAATAG ATGAATGCCA ATCCATCAGG TCACATGCAA ATACTCCCAG ACCTGATCAG 
AAAACTGAGT TGAAATCTGA TGTGGATGAA GATCTAAAAG AAATGGTAAA AAGTATCGAC 
GAGATGCTTA ATAACGCAAA CGATAGTTTC GAGTTGATTG AATCGTTCGA GCCTGATACA 
ACACAGATGG AATTGAATTT TTATGAACCA AATATCCCAA GAGGACCACC TCCAAACTTG 
CTTGAATTAT TGGACAGTTG TCACGATATC GTAAACTGTT TAGAAAATTC ATCATGTGCA 
AGTTCCGAGT GTGGTGAGGG AAGTGTCGAG TCACCTTTAA AACGAAGGAA GAAGAGGAAA 
AATAGAACCG GATGGCCTGG AATTAAAATG AAGAGAAGAC TCCAAAGCAA ACCTCTGGCA 
GACATAAACT GTGACAGGGA AAACCTAGTG GAGAAAAATA TAGGGCAAAG CGAGAAGGAT 
TGTAATGTGC AGGTCACGAA TATGTCTAAC AGATTAACGG AAGAAGGTAA TATTAAAATA 
GCTACATCCG TTACGAGTTC CATCACACAA GATGAACAGT CATTAGGCTT CGGCCAGAGG 
AAGGACGACG GACGTTTATT CGAAGTGTAT ACCTCGAATA TTTCTTCTAA CACGCGTCAC 
GACGAAAACG AGAAGAAAGA CGTAAGTATA GCGGTTCCTG ATAATTCCAT GTTACATACA 
ACAAATGCTG AATCCTGCAC AGGTTCACTT ACATCTGATA TATGCAACGA GACTGACAGG 
AATTTCATAT TGAACAAAGA CTATGGATTC AGAAAAAATT TGTCGAAGAT CGAAGAAATG 
TCAGAAAATG ATCACGGAAA CGATGAAAAT CACGAGAACG TGTTTCGGAA AGATGTTCAT 
TCTATATCGG AACGTCGTTT TATCTCGAAC GCAACCATCA AGAAACGACA ACATGACAAC 
ACATCTTCTG AAACTTGCGA ATCTCGTGTA GAACTCGAAA ATCATGAGAT ATTGTGTAGA 
AAGGACGCCG AGACCAAAAT TGTTTCCAGG AAACATCATA ATACTAACGG ATTACCGAGA 
AAGAGGCAAC AGAAGACTCA TTCTGAAAAC TCTGACTCTG AAATTGAACA GCATCATCGC 
CTCCATCATC ATCACCATCA CCACCTTCAC CATAGTAATG TATATAAAAG GAACAGAGTA 
ACTTCGCGAA AACGAATTTG CGCGAAGAAA CGGACGAGAA AAACTGACAT ATCTTCAGAT 
ACAGTGTTCG AAGATGAGAA TTGTAAAAAG GATTCCTATT TAAGTATTAC ATGTAGAAAG 
CAACAAACTA TAAAAGGTAC AAAACGACGA GCCGATACCA TGTCATCGGA AACACAAAAT 
TCTGAAGTCG ATTGTGCATT ATCGGGACAT GTCAGTCCTA CGATTATAAC TACTTCAGAA 
CTTGAACAAA GACGTTCGAG TATTGAGTTT CAACCAGTTG TTAGAATGAT GAAGATTGAC 
GATCAAGTTG AAATGGACCA CAGTATTCTC TCGGTAACGA TTGCAAGCAA CAGACGGTTA 
AGAAGTTCCA GTTCTCCGAG ATCGAATACT CAACCGCCGA AAAAACGTTT AAAGACCAAT 
CGTGGTCAAT TCGGAAGGTG GTTAAAAAGT AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003489702.1
CDS	242..4897
Translation	MVSPSKIQHQEHKQPSRENKLNKHSHVHTKKELKSLEGKSFYLDIKNHATTTKIEAKIKDLGGVIELFLIRSVDLVISDRVDKTGYINFEKHKWGCASGGSGGPPSLRSLETPTPMPTPPTSFFSPECPLSNTTNLRGQTTQRSKSRVEAMLERALIQPQQCSVDPLYNAQNWGIPIWAIDKLQSWLDKIYSSVKDTNHLKQLRHSHQQSSTKDIKVRHLKGPYLKFESFQRNTRPVFVELPVWPTLNFHGDPGSCPFNTKRREKLEKLGKEVKENREGIPAINQEEKKEMTRRPRATVRTRRTEQLASGYCEICRIRYRDLAKHVQSDQHLSFIQNDDNFLSLDKLINAGANVEAFLKLNRGKNIEKDCNLFSNGDRNLHDTVLPEGKVNRNAKSLGDFDVGDIKMVQRNGARRNLSLRLNSSHNLRTRTRHESGHLLRSKGSPWHEADKTEKFYDEFEGFTIKKRAKGTIWIEEDAPGDKYIEEHELKESKQNEYKIKTFSAELEEKCCNEKNCETYNKLKDTNNQDIQRTINCNNEENSIGNDPERIKQEQTHPKSKNHDQINGNIKNGCNENLLSTNDNSRIKPCKVSPGNETLKELTCKLQLENTPSSAKRLSEHNDINTGTICNGHSVRDEQKINRTLNNTEKNEVAKEEKSKCCKSNRRGSRSVRGRHRLSVEERLIEDNRAYYKVEVLGSKLRSSVLSNNNSQCTVQKDGDSEEKKEVPSSEKPVVVRFKRVRKSELSLLSDEAESFMFRELKRDDSSEISDDEQSSVLPRDTESECNDSGNSICPSSFLTSSSPAKSETTEDDSQDSLSLGRARKKRRTQAEALIKDNVDYYKFETPGSRLRYQAPLTGIKENQEEIEGSIVEKGIEIAKVYPSKPSPEVEKMQFSFEAVPKSEPWYQTYQRQDEGAEFWHYFSEGDSQKPFLLPYEIENFHEILIKNQNRAENKRKGRGRSIGCIGRSPRKSPRCHASTLAIMSTIIRKREQQQQQSSNLYTIDECQSIRSHANTPRPDQKTELKSDVDEDLKEMVKSIDEMLNNANDSFELIESFEPDTTQMELNFYEPNIPRGPPPNLLELLDSCHDIVNCLENSSCASSECGEGSVESPLKRRKKRKNRTGWPGIKMKRRLQSKPLADINCDRENLVEKNIGQSEKDCNVQVTNMSNRLTEEGNIKIATSVTSSITQDEQSLGFGQRKDDGRLFEVYTSNISSNTRHDENEKKDVSIAVPDNSMLHTTNAESCTGSLTSDICNETDRNFILNKDYGFRKNLSKIEEMSENDHGNDENHENVFRKDVHSISERRFISNATIKKRQHDNTSSETCESRVELENHEILCRKDAETKIVSRKHHNTNGLPRKRQQKTHSENSDSEIEQHHRLHHHHHHHLHHSNVYKRNRVTSRKRICAKKRTRKTDISSDTVFEDENCKKDSYLSITCRKQQTIKGTKRRADTMSSETQNSEVDCALSGHVSPTIITTSELEQRRSSIEFQPVVRMMKIDDQVEMDHSILSVTIASNRRLRSSSSPRSNTQPPKKRLKTNRGQFGRWLKSS

Target ORF information:

RefSeq Version	XM_003489654.2
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens uncharacterized LOC100745898 (LOC100745898), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003489654.2

ORF Insert Sequence:

ATGGTATCGC CATCCAAAAT TCAACATCAG GAGCATAAGC AGCCATCAAG AGAAAATAAA 
TTAAACAAAC ACTCACATGT TCATACAAAG AAGGAATTGA AATCATTGGA AGGAAAGTCG 
TTTTACTTGG ATATCAAGAA TCATGCTACT ACAACTAAAA TAGAAGCTAA GATTAAAGAT 
TTGGGAGGAG TAATAGAATT ATTTTTAATA CGGAGTGTAG ACCTGGTGAT TAGTGACAGA 
GTAGACAAGA CTGGTTACAT AAATTTTGAA AAACATAAAT GGGGTTGTGC CAGTGGAGGC 
AGCGGGGGAC CTCCCTCGCT GAGGAGTTTA GAAACTCCAA CCCCCATGCC AACTCCTCCA 
ACATCATTTT TTAGTCCTGA ATGCCCCTTG TCTAATACAA CCAATCTACG GGGTCAAACT 
ACCCAAAGAT CTAAATCGCG GGTGGAGGCA ATGTTGGAGC GTGCTCTGAT ACAGCCACAA 
CAGTGTTCCG TGGATCCACT CTACAACGCT CAAAATTGGG GAATTCCTAT TTGGGCAATT 
GATAAACTTC AGTCTTGGCT TGACAAAATC TACTCATCTG TTAAAGACAC GAATCATTTG 
AAACAATTAA GACACTCACA TCAACAGAGT TCAACTAAAG ATATAAAGGT CAGACACCTC 
AAGGGTCCTT ATTTGAAATT TGAATCCTTT CAGAGGAATA CTAGACCTGT ATTTGTTGAA 
CTACCTGTGT GGCCAACATT GAATTTTCAC GGTGATCCAG GCTCTTGTCC TTTCAATACG 
AAGAGACGAG AAAAACTGGA AAAATTAGGG AAAGAAGTAA AAGAAAACAG GGAAGGTATT 
CCGGCTATCA ACCAAGAGGA GAAAAAAGAA ATGACTCGGC GACCACGAGC AACTGTCCGC 
ACTCGAAGAA CAGAACAATT AGCTTCCGGT TATTGTGAAA TTTGCCGTAT TAGATATAGA 
GATCTGGCCA AACATGTGCA AAGCGATCAG CATCTTAGCT TTATTCAAAA TGATGATAAT 
TTCTTGTCTT TGGATAAATT GATCAATGCA GGAGCCAATG TAGAAGCGTT TTTAAAACTA 
AACAGAGGAA AAAATATAGA AAAAGATTGC AATTTGTTTT CCAATGGGGA TCGTAATCTT 
CATGACACAG TATTGCCAGA AGGGAAGGTT AATAGAAATG CAAAAAGTTT AGGGGATTTT 
GACGTTGGAG ACATAAAGAT GGTACAACGT AATGGCGCAC GTCGGAATCT CAGTTTAAGA 
TTAAACTCCT CTCATAATTT ACGTACACGT ACAAGACACG AAAGCGGCCA TTTATTAAGA 
TCAAAAGGTA GTCCCTGGCA TGAAGCCGAT AAAACGGAGA AGTTCTACGA CGAATTCGAA 
GGTTTTACTA TCAAAAAGCG AGCGAAAGGG ACAATTTGGA TCGAAGAAGA TGCACCAGGA 
GACAAATATA TAGAAGAACA TGAATTAAAA GAATCTAAGC AAAATGAATA CAAAATTAAA 
ACATTTTCGG CGGAATTAGA AGAAAAATGT TGTAATGAGA AAAATTGTGA AACATATAAC 
AAACTTAAAG ATACTAACAA TCAGGACATA CAAAGGACAA TAAATTGTAA TAACGAAGAA 
AATAGTATTG GAAATGACCC TGAACGCATC AAACAAGAAC AAACTCATCC AAAGAGTAAA 
AATCATGATC AAATTAATGG AAACATAAAA AATGGTTGTA ATGAAAATCT TTTAAGTACA 
AATGATAACA GTCGTATTAA ACCTTGTAAA GTTTCGCCTG GTAACGAAAC TTTAAAAGAA 
TTGACTTGCA AATTACAGTT AGAAAATACT CCATCAAGTG CAAAAAGGTT ATCAGAGCAC 
AATGATATCA ATACAGGCAC AATCTGTAAT GGTCATTCTG TAAGGGATGA ACAGAAAATT 
AATAGAACAC TGAATAATAC AGAAAAGAAC GAAGTAGCAA AAGAAGAGAA ATCAAAATGT 
TGCAAGTCAA ATAGAAGAGG TAGTCGGAGC GTCAGAGGAC GGCACAGGTT GTCCGTTGAA 
GAACGTTTGA TTGAAGATAA TCGTGCATAT TATAAAGTGG AAGTGTTAGG AAGTAAGTTA 
AGATCAAGTG TATTGTCAAA CAATAATTCT CAATGTACAG TTCAAAAGGA TGGGGATAGT 
GAAGAAAAGA AGGAAGTGCC ATCTAGTGAG AAACCAGTAG TTGTACGATT TAAACGTGTA 
AGAAAGTCGG AACTATCATT ACTAAGCGAT GAAGCGGAGT CTTTTATGTT TAGGGAACTT 
AAACGAGATG ATTCAAGTGA AATAAGCGAT GACGAACAGA GTAGCGTATT ACCAAGGGAT 
ACTGAAAGTG AGTGCAACGA TAGCGGCAAT TCTATCTGTC CTAGTTCATT CTTAACTTCT 
AGTTCACCTG CAAAATCGGA AACTACCGAA GACGATTCTC AAGACTCTTT AAGTTTGGGA 
CGAGCTAGAA AAAAAAGACG GACACAAGCA GAAGCGCTGA TCAAAGATAA TGTTGATTAT 
TACAAATTCG AAACTCCTGG GAGCAGATTA AGGTATCAGG CGCCTCTAAC TGGTATTAAA 
GAAAATCAGG AAGAAATAGA AGGTAGTATT GTCGAGAAAG GTATAGAAAT AGCAAAGGTG 
TATCCGTCCA AACCCTCACC AGAAGTTGAA AAAATGCAAT TTTCTTTTGA AGCCGTACCA 
AAGTCTGAAC CATGGTATCA AACGTATCAG CGGCAAGATG AAGGAGCAGA ATTTTGGCAC 
TATTTTAGCG AAGGGGACTC ACAAAAGCCA TTTCTTTTAC CATATGAAAT TGAAAATTTT 
CACGAAATTT TGATAAAAAA CCAAAATAGA GCGGAAAATA AAAGAAAAGG TCGCGGACGG 
AGTATAGGTT GTATTGGACG ATCTCCTAGG AAAAGTCCTC GTTGTCATGC TTCCACATTA 
GCTATTATGT CAACAATTAT AAGAAAAAGA GAACAACAGC AACAACAATC TTCGAATTTG 
TACACAATAG ATGAATGCCA ATCCATCAGG TCACATGCAA ATACTCCCAG ACCTGATCAG 
AAAACTGAGT TGAAATCTGA TGTGGATGAA GATCTAAAAG AAATGGTAAA AAGTATCGAC 
GAGATGCTTA ATAACGCAAA CGATAGTTTC GAGTTGATTG AATCGTTCGA GCCTGATACA 
ACACAGATGG AATTGAATTT TTATGAACCA AATATCCCAA GAGGACCACC TCCAAACTTG 
CTTGAATTAT TGGACAGTTG TCACGATATC GTAAACTGTT TAGAAAATTC ATCATGTGCA 
AGTTCCGAGT GTGGTGAGGG AAGTGTCGAG TCACCTTTAA AACGAAGGAA GAAGAGGAAA 
AATAGAACCG GATGGCCTGG AATTAAAATG AAGAGAAGAC TCCAAAGCAA ACCTCTGGCA 
GACATAAACT GTGACAGGGA AAACCTAGTG GAGAAAAATA TAGGGCAAAG CGAGAAGGAT 
TGTAATGTGC AGGTCACGAA TATGTCTAAC AGATTAACGG AAGAAGGTAA TATTAAAATA 
GCTACATCCG TTACGAGTTC CATCACACAA GATGAACAGT CATTAGGCTT CGGCCAGAGG 
AAGGACGACG GACGTTTATT CGAAGTGTAT ACCTCGAATA TTTCTTCTAA CACGCGTCAC 
GACGAAAACG AGAAGAAAGA CGTAAGTATA GCGGTTCCTG ATAATTCCAT GTTACATACA 
ACAAATGCTG AATCCTGCAC AGGTTCACTT ACATCTGATA TATGCAACGA GACTGACAGG 
AATTTCATAT TGAACAAAGA CTATGGATTC AGAAAAAATT TGTCGAAGAT CGAAGAAATG 
TCAGAAAATG ATCACGGAAA CGATGAAAAT CACGAGAACG TGTTTCGGAA AGATGTTCAT 
TCTATATCGG AACGTCGTTT TATCTCGAAC GCAACCATCA AGAAACGACA ACATGACAAC 
ACATCTTCTG AAACTTGCGA ATCTCGTGTA GAACTCGAAA ATCATGAGAT ATTGTGTAGA 
AAGGACGCCG AGACCAAAAT TGTTTCCAGG AAACATCATA ATACTAACGG ATTACCGAGA 
AAGAGGCAAC AGAAGACTCA TTCTGAAAAC TCTGACTCTG AAATTGAACA GCATCATCGC 
CTCCATCATC ATCACCATCA CCACCTTCAC CATAGTAATG TATATAAAAG GAACAGAGTA 
ACTTCGCGAA AACGAATTTG CGCGAAGAAA CGGACGAGAA AAACTGACAT ATCTTCAGAT 
ACAGTGTTCG AAGATGAGAA TTGTAAAAAG GATTCCTATT TAAGTATTAC ATGTAGAAAG 
CAACAAACTA TAAAAGGTAC AAAACGACGA GCCGATACCA TGTCATCGGA AACACAAAAT 
TCTGAAGTCG ATTGTGCATT ATCGGGACAT GTCAGTCCTA CGATTATAAC TACTTCAGAA 
CTTGAACAAA GACGTTCGAG TATTGAGTTT CAACCAGTTG TTAGAATGAT GAAGATTGAC 
GATCAAGTTG AAATGGACCA CAGTATTCTC TCGGTAACGA TTGCAAGCAA CAGACGGTTA 
AGAAGTTCCA GTTCTCCGAG ATCGAATACT CAACCGCCGA AAAAACGTTT AAAGACCAAT 
CGTGGTCAAT TCGGAAGGTG GTTAAAAAGT AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC100745898 cDNA ORF clone, Bombus impatiens(common eastern bumble bee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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