ITSN1 cDNA ORF clone, Bison bison bison

Gene
Clones
gRNAs

Summary

Gene Symbol	ITSN1
Entrez Gene ID	105004611
Full Name	intersectin 1
Gene Type	protein-coding
Organism	Bison bison bison

mRNA and Protein(s)

mRNA	Protein	Name
XM_010862262.1	XP_010860564.1	intersectin-1 isoform X2
XM_010862263.1	XP_010860565.1	intersectin-1 isoform X3
XM_010862264.1	XP_010860566.1	intersectin-1 isoform X4
XM_010862259.1	XP_010860561.1	intersectin-1 isoform X1
XM_010862260.1	XP_010860562.1	intersectin-1 isoform X1
XM_010862261.1	XP_010860563.1	intersectin-1 isoform X1
XM_010862266.1	XP_010860568.1	intersectin-1 isoform X5
XM_010862265.1	XP_010860567.1	intersectin-1 isoform X5

Pathways from BioSystems
Gene Ontology

The following ITSN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ITSN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBi81221	XM_010862264.1 Latest version!	Bison bison bison intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBi81222	XM_010862266.1 Latest version!	Bison bison bison intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBi81222	XM_010862265.1 Latest version!	Bison bison bison intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBi81218	XM_010862261.1 Latest version!	Bison bison bison intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBi81218	XM_010862259.1 Latest version!	Bison bison bison intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBi81218	XM_010862260.1 Latest version!	Bison bison bison intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBi81219	XM_010862262.1 Latest version!	Bison bison bison intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBi81220	XM_010862263.1 Latest version!	Bison bison bison intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ITSN1 ( XM_010862264.1 ) cDNA ORF clone, Bison bison bison -> XP_010860566.1 Bison bison bison intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBi81221
Clone ID Related Accession (Same CDS sequence)	XM_010862264.1
Accession Version	XM_010862264.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	intersectin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011495041.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCCACACC TTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGGTATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCCGTTGTT 
GGGATGTCTC CCCCCCTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT 
GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCA TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACG 
ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT 
GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA 
GAAAACGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC 
AAAGTGGCTG TGCGTGAGAC CCCCGCCCCT TCACCCGCAA CCCCTGCAGA GCCCTCCGAG 
ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCATGAA TGAGAAACCA 
GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG 
CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT 
GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCGC AAGCCCTGTA TCCGTGGAGA 
GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGATG TCATCACCGT ACTGGAGCAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TCCCTCAGAA 
AGTCCTGCAA GTCTAAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCCGT GTCGGGAGAA 
GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GCGATTTAAC CTTTCAGCAA 
GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC 
AAGTCCGGCG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCGGAGGG CTCTGGAACT 
GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATCG CGCAGGTCAT TGCCTCCTAC 
ACAGCTACGG GTCCTGAACA GCTTACCCTG GCCCCCGGTC AGCTGATCCT GATCCGGAAA 
AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTACAGGCAC GTGGGAAAAA GCGCCAGATA 
GGCTGGTTTC CTGCTAATTA CGTCAAACTT TTAAGCCCTG GGACAAGCAA AATCACTCCA 
ACAGACCCAC CGAAGCCAAC AGCTTTCCCA ACAGTGTGCC AGGTGATCGG GATGTACGAC 
TACACCGCGC AGAATGACGA CGAGCTGGCC TTCAGCAAGG GCCAGGTCAT CAATGTCCTC 
AACAAGGAGG ACCCCGACTG GTGGAAGGGT GAAGTCCACG GGCAGGTGGG GCTCTTCCCG 
TCCAACTACG TGAAGTTGAC CACGGACACG GACCCCAGCC AGCAATGGTG CTCAGACTTA 
CACCTCTTGG ATATGCTGAC CCCAACTGAA AGAAAGCGAC AGGGGTACAT CCACGAGCTC 
ATCGTCACGG AGGAGAACTA CGTGAATGAC CTGCAGCTGG TCACAGAGAG CGCTGAGGGT 
CCGCAAGAAG ATGTCTGGGG AGAAGATGCC CGTGAAGATG ATCGGAGACA TCCTGACGGC 
CCAGCTGCCA CACATGCAGC CCTACATCCG CTTCTGCAGC TGCCAGCTCA ACGGGGCCGC 
CTTGATCCAG CAGAAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010860566.1
CDS	304..4224
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPPLVSSVPAAGVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPTAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVITSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLLTLKRALEAKELARQQLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSIEAERLKQKEQERKILELEKQKEEAQRRAQERDQQWLERMHQEDEQQRPRKPHEEEKLKREESVKKKDSEEKGKPEMQDKLSRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENIKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTEAASTPTPKVAVRETPAPSPATPAEPSETPNNWADFSSTWPTSMNEKPETDSWDAWATQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGPSESPASLKRVASPAAKPAVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKSGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTDPPKPTAFPTVCQVIGMYDYTAQNDDELAFSKGQVINVLNKEDPDWWKGEVHGQVGLFPSNYVKLTTDTDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTESAEGPQEDVWGEDAREDDRRHPDGPAATHAALHPLLQLPAQRGRLDPAED

Target ORF information:

RefSeq Version	XM_010862264.1
Organism	Bison bison bison
Definition	Bison bison bison intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010862264.1

ORF Insert Sequence:

ATGGCTCAGT TTCCCACACC TTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGGTATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCCGTTGTT 
GGGATGTCTC CCCCCCTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT 
GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCA TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACG 
ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT 
GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA 
GAAAACGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC 
AAAGTGGCTG TGCGTGAGAC CCCCGCCCCT TCACCCGCAA CCCCTGCAGA GCCCTCCGAG 
ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCATGAA TGAGAAACCA 
GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG 
CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT 
GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCGC AAGCCCTGTA TCCGTGGAGA 
GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGATG TCATCACCGT ACTGGAGCAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TCCCTCAGAA 
AGTCCTGCAA GTCTAAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCCGT GTCGGGAGAA 
GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GCGATTTAAC CTTTCAGCAA 
GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC 
AAGTCCGGCG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCGGAGGG CTCTGGAACT 
GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATCG CGCAGGTCAT TGCCTCCTAC 
ACAGCTACGG GTCCTGAACA GCTTACCCTG GCCCCCGGTC AGCTGATCCT GATCCGGAAA 
AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTACAGGCAC GTGGGAAAAA GCGCCAGATA 
GGCTGGTTTC CTGCTAATTA CGTCAAACTT TTAAGCCCTG GGACAAGCAA AATCACTCCA 
ACAGACCCAC CGAAGCCAAC AGCTTTCCCA ACAGTGTGCC AGGTGATCGG GATGTACGAC 
TACACCGCGC AGAATGACGA CGAGCTGGCC TTCAGCAAGG GCCAGGTCAT CAATGTCCTC 
AACAAGGAGG ACCCCGACTG GTGGAAGGGT GAAGTCCACG GGCAGGTGGG GCTCTTCCCG 
TCCAACTACG TGAAGTTGAC CACGGACACG GACCCCAGCC AGCAATGGTG CTCAGACTTA 
CACCTCTTGG ATATGCTGAC CCCAACTGAA AGAAAGCGAC AGGGGTACAT CCACGAGCTC 
ATCGTCACGG AGGAGAACTA CGTGAATGAC CTGCAGCTGG TCACAGAGAG CGCTGAGGGT 
CCGCAAGAAG ATGTCTGGGG AGAAGATGCC CGTGAAGATG ATCGGAGACA TCCTGACGGC 
CCAGCTGCCA CACATGCAGC CCTACATCCG CTTCTGCAGC TGCCAGCTCA ACGGGGCCGC 
CTTGATCCAG CAGAAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_010862266.1 ) cDNA ORF clone, Bison bison bison -> XP_010860568.1 Bison bison bison intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBi81222
Clone ID Related Accession (Same CDS sequence)	XM_010862266.1 , XM_010862265.1
Accession Version	XM_010862266.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	intersectin-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011495041.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCCACACC TTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGGTATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCCGTTGTT 
GGGATGTCTC CCCCCCTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT 
GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCA TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACG 
ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT 
GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA 
GAAAACGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC 
AAAGTGGCTG TGCGTGAGAC CCCCGCCCCT TCACCCGCAA CCCCTGCAGA GCCCTCCGAG 
ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCATGAA TGAGAAACCA 
GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG 
CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT 
GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCGC AAGCCCTGTA TCCGTGGAGA 
GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGATG TCATCACCGT ACTGGAGCAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TCCCTCAGAA 
AGTCCTGCAA GTCTAAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCCGT GTCGGGAGAA 
GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GCGATTTAAC CTTTCAGCAA 
GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC 
AAGTCCGGCG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCGGAGGG CTCTGGAACT 
GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATCG CGCAGGTCAT TGCCTCCTAC 
ACAGCTACGG GTCCTGAACA GCTTACCCTG GCCCCCGGTC AGCTGATCCT GATCCGGAAA 
AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTACAGGCAC GTGGGAAAAA GCGCCAGATA 
GGCTGGTTTC CTGCTAATTA CGTCAAACTT TTAAGCCCTG GGACAAGCAA AATCACTCCA 
ACAGACCCAC CGAAGCCAAC AGCTTTCCCA ACAGTGTGCC AGGTGATCGG GATGTACGAC 
TACACCGCGC AGAATGACGA CGAGCTGGCC TTCAGCAAGG GCCAGGTCAT CAATGTCCTC 
AACAAGGAGG ACCCCGACTG GTGGAAGGGT GAAGTCCACG GGCAGGTGGG GCTCTTCCCG 
TCCAACTACG TGAAGTTGAC CACGGACACG GACCCCAGCC AGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010860568.1
CDS	304..3951
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPPLVSSVPAAGVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPTAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVITSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLLTLKRALEAKELARQQLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSIEAERLKQKEQERKILELEKQKEEAQRRAQERDQQWLERMHQEDEQQRPRKPHEEEKLKREESVKKKDSEEKGKPEMQDKLSRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENIKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTEAASTPTPKVAVRETPAPSPATPAEPSETPNNWADFSSTWPTSMNEKPETDSWDAWATQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGPSESPASLKRVASPAAKPAVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKSGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTDPPKPTAFPTVCQVIGMYDYTAQNDDELAFSKGQVINVLNKEDPDWWKGEVHGQVGLFPSNYVKLTTDTDPSQQ

Target ORF information:

RefSeq Version	XM_010862266.1
Organism	Bison bison bison
Definition	Bison bison bison intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010862266.1

ORF Insert Sequence:

ATGGCTCAGT TTCCCACACC TTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGGTATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCCGTTGTT 
GGGATGTCTC CCCCCCTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT 
GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCA TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACG 
ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT 
GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA 
GAAAACGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC 
AAAGTGGCTG TGCGTGAGAC CCCCGCCCCT TCACCCGCAA CCCCTGCAGA GCCCTCCGAG 
ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCATGAA TGAGAAACCA 
GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG 
CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT 
GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCGC AAGCCCTGTA TCCGTGGAGA 
GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGATG TCATCACCGT ACTGGAGCAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TCCCTCAGAA 
AGTCCTGCAA GTCTAAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCCGT GTCGGGAGAA 
GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GCGATTTAAC CTTTCAGCAA 
GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC 
AAGTCCGGCG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCGGAGGG CTCTGGAACT 
GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATCG CGCAGGTCAT TGCCTCCTAC 
ACAGCTACGG GTCCTGAACA GCTTACCCTG GCCCCCGGTC AGCTGATCCT GATCCGGAAA 
AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTACAGGCAC GTGGGAAAAA GCGCCAGATA 
GGCTGGTTTC CTGCTAATTA CGTCAAACTT TTAAGCCCTG GGACAAGCAA AATCACTCCA 
ACAGACCCAC CGAAGCCAAC AGCTTTCCCA ACAGTGTGCC AGGTGATCGG GATGTACGAC 
TACACCGCGC AGAATGACGA CGAGCTGGCC TTCAGCAAGG GCCAGGTCAT CAATGTCCTC 
AACAAGGAGG ACCCCGACTG GTGGAAGGGT GAAGTCCACG GGCAGGTGGG GCTCTTCCCG 
TCCAACTACG TGAAGTTGAC CACGGACACG GACCCCAGCC AGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_010862265.1 ) cDNA ORF clone, Bison bison bison -> XP_010860567.1 Bison bison bison intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X7, mRNA.

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CloneID	OBi81222
Clone ID Related Accession (Same CDS sequence)	XM_010862266.1 , XM_010862265.1
Accession Version	XM_010862265.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	intersectin-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011495041.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCCACACC TTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGGTATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCCGTTGTT 
GGGATGTCTC CCCCCCTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT 
GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCA TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACG 
ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT 
GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA 
GAAAACGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC 
AAAGTGGCTG TGCGTGAGAC CCCCGCCCCT TCACCCGCAA CCCCTGCAGA GCCCTCCGAG 
ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCATGAA TGAGAAACCA 
GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG 
CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT 
GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCGC AAGCCCTGTA TCCGTGGAGA 
GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGATG TCATCACCGT ACTGGAGCAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TCCCTCAGAA 
AGTCCTGCAA GTCTAAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCCGT GTCGGGAGAA 
GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GCGATTTAAC CTTTCAGCAA 
GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC 
AAGTCCGGCG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCGGAGGG CTCTGGAACT 
GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATCG CGCAGGTCAT TGCCTCCTAC 
ACAGCTACGG GTCCTGAACA GCTTACCCTG GCCCCCGGTC AGCTGATCCT GATCCGGAAA 
AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTACAGGCAC GTGGGAAAAA GCGCCAGATA 
GGCTGGTTTC CTGCTAATTA CGTCAAACTT TTAAGCCCTG GGACAAGCAA AATCACTCCA 
ACAGACCCAC CGAAGCCAAC AGCTTTCCCA ACAGTGTGCC AGGTGATCGG GATGTACGAC 
TACACCGCGC AGAATGACGA CGAGCTGGCC TTCAGCAAGG GCCAGGTCAT CAATGTCCTC 
AACAAGGAGG ACCCCGACTG GTGGAAGGGT GAAGTCCACG GGCAGGTGGG GCTCTTCCCG 
TCCAACTACG TGAAGTTGAC CACGGACACG GACCCCAGCC AGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010860567.1
CDS	304..3951
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPPLVSSVPAAGVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPTAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVITSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLLTLKRALEAKELARQQLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSIEAERLKQKEQERKILELEKQKEEAQRRAQERDQQWLERMHQEDEQQRPRKPHEEEKLKREESVKKKDSEEKGKPEMQDKLSRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENIKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTEAASTPTPKVAVRETPAPSPATPAEPSETPNNWADFSSTWPTSMNEKPETDSWDAWATQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGPSESPASLKRVASPAAKPAVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKSGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTDPPKPTAFPTVCQVIGMYDYTAQNDDELAFSKGQVINVLNKEDPDWWKGEVHGQVGLFPSNYVKLTTDTDPSQQ

Target ORF information:

RefSeq Version	XM_010862265.1
Organism	Bison bison bison
Definition	Bison bison bison intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010862265.1

ORF Insert Sequence:

ATGGCTCAGT TTCCCACACC TTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGGTATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCCGTTGTT 
GGGATGTCTC CCCCCCTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT 
GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCA TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACG 
ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT 
GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA 
GAAAACGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC 
AAAGTGGCTG TGCGTGAGAC CCCCGCCCCT TCACCCGCAA CCCCTGCAGA GCCCTCCGAG 
ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCATGAA TGAGAAACCA 
GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG 
CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT 
GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCGC AAGCCCTGTA TCCGTGGAGA 
GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGATG TCATCACCGT ACTGGAGCAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TCCCTCAGAA 
AGTCCTGCAA GTCTAAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCCGT GTCGGGAGAA 
GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GCGATTTAAC CTTTCAGCAA 
GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC 
AAGTCCGGCG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCGGAGGG CTCTGGAACT 
GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATCG CGCAGGTCAT TGCCTCCTAC 
ACAGCTACGG GTCCTGAACA GCTTACCCTG GCCCCCGGTC AGCTGATCCT GATCCGGAAA 
AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTACAGGCAC GTGGGAAAAA GCGCCAGATA 
GGCTGGTTTC CTGCTAATTA CGTCAAACTT TTAAGCCCTG GGACAAGCAA AATCACTCCA 
ACAGACCCAC CGAAGCCAAC AGCTTTCCCA ACAGTGTGCC AGGTGATCGG GATGTACGAC 
TACACCGCGC AGAATGACGA CGAGCTGGCC TTCAGCAAGG GCCAGGTCAT CAATGTCCTC 
AACAAGGAGG ACCCCGACTG GTGGAAGGGT GAAGTCCACG GGCAGGTGGG GCTCTTCCCG 
TCCAACTACG TGAAGTTGAC CACGGACACG GACCCCAGCC AGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_010862259.1 ) cDNA ORF clone, Bison bison bison -> XP_010860561.1 Bison bison bison intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBi81218
Clone ID Related Accession (Same CDS sequence)	XM_010862259.1 , XM_010862260.1 , XM_010862261.1
Accession Version	XM_010862259.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	intersectin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011495041.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCCACACC TTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGGTATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCCGTTGTT 
GGGATGTCTC CCCCCCTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT 
GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCA TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACG 
ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT 
GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA 
GAAAACGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC 
AAAGTGGCTG TGCGTGAGAC CCCCGCCCCT TCACCCGCAA CCCCTGCAGA GCCCTCCGAG 
ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCATGAA TGAGAAACCA 
GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG 
CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT 
GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCGC AAGCCCTGTA TCCGTGGAGA 
GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGATG TCATCACCGT ACTGGAGCAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TCCCTCAGAA 
AGTCCTGCAA GTCTAAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCCGT GTCGGGAGAA 
GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GCGATTTAAC CTTTCAGCAA 
GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC 
AAGTCCGGCG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCGGAGGG CTCTGGAACT 
GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATCG CGCAGGTCAT TGCCTCCTAC 
ACAGCTACGG GTCCTGAACA GCTTACCCTG GCCCCCGGTC AGCTGATCCT GATCCGGAAA 
AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTACAGGCAC GTGGGAAAAA GCGCCAGATA 
GGCTGGTTTC CTGCTAATTA CGTCAAACTT TTAAGCCCTG GGACAAGCAA AATCACTCCA 
ACAGACCCAC CGAAGCCAAC AGCTTTCCCA ACAGTGTGCC AGGTGATCGG GATGTACGAC 
TACACCGCGC AGAATGACGA CGAGCTGGCC TTCAGCAAGG GCCAGGTCAT CAATGTCCTC 
AACAAGGAGG ACCCCGACTG GTGGAAGGGT GAAGTCCACG GGCAGGTGGG GCTCTTCCCG 
TCCAACTACG TGAAGTTGAC CACGGACACG GACCCCAGCC AGCAATGGTG CTCAGACTTA 
CACCTCTTGG ATATGCTGAC CCCAACTGAA AGAAAGCGAC AGGGGTACAT CCACGAGCTC 
ATCGTCACGG AGGAGAACTA CGTGAATGAC CTGCAGCTGG TCACAGAGAT CTTTCAGAAA 
CCCCTGATGG AGTCTGAGCT GCTGACAGAA AAAGAGGGTG CTATGATTTT TGTTAACTGG 
AAGGAGCTGA TTATGTGTAA TATCAAACTA CTGAAAGCGC TGAGGGTCCG CAAGAAGATG 
TCTGGGGAGA AGATGCCCGT GAAGATGATC GGAGACATCC TGACGGCCCA GCTGCCACAC 
ATGCAGCCCT ACATCCGCTT CTGCAGCTGC CAGCTCAACG GGGCCGCCTT GATCCAGCAG 
AAGACTGACG AGGCCCCAGA CTTCAAGGAG TTTGTCAAAA GACTGGCCAT GGACCCACGC 
TGTAAGGGAA TGCCCCTGTC TAGTTTTATA CTGAAGCCTA TGCAACGAGT AACGAGATAC 
CCACTGATCA TTAAAAATAT CCTGGAAAAC ACTCCTGAAA ACCACCCAGA CCACAGCCAC 
CTGAAGCACG CCCTGGAGAA GGCGGAGGAG CTGTGCTCCC AGGTGAACGA AGGGGTGCGG 
GAGAAGGAGA ACTCGGACCG ACTGGAGTGG ATCCAGGCCC ACGTGCTGTG TGAAGGCCTG 
TCGGAGCAAC TTGTGTTCAA TTCCGTGACC AACTGCCTGG GGCCGCGCAA GTTCCTACAC 
AGTGGGAAGC TCTACAAGGC CAAGAGCAAC AAGGAGCTGT ACGGCTTCCT CTTCAACGAC 
TTCCTGCTGC TGACCCAAAT CATCAAGCCC CTGGGCTCCT CGGGCACCGA CAAAGTCTTC 
AGCCCCAAAT CAAACTTGCA GTATAAAATG TACAAAACAC CTATTTTCCT AAATGAGGTT 
CTAGTGAAGT TGCCCACTGA CCCTTCTGGG GATGAACCCA TCTTCCACAT TTCCCACATT 
GACCGAGTCT ACACCCTCCG AGCAGAGAGC ATAAACGAAA GGACTGCCTG GGTGCAGAAA 
ATCAAAGCAG CTTCTGAACT CTACATAGAG ACAGAGAAGA AGAAGCGGGA GAAGGCGTAC 
CTGGTCCGCT CCCAAAGAGC CACAGGCATT GGAAGGTTGA TGGTGAACGT GGTTGAAGGC 
ATTGAGCTGA AACCCTGTCG CTCCCATGGA AAGAGTAACC CGTACTGCGA GGTGACCATG 
GGCTCCCAGT GTCACATCAC CAAGACGATC CAGGACACTC TGAATCCCAA GTGGAATTCC 
AACTGCCAGT TCTTCATCAG AGACCTGGAG CAGGAGGTGC TGTGCATCAC TGTGTTTGAG 
AGGGACCAGT TCTCGCCAGA TGATTTTTTG GGTCGGACGG AGATCCGCGT GGCCGACATC 
AAGAAGGACC AGGGCTCCAA GGGTCCCGTT ACGAAGTGTC TCCTGCTGCA CGAAGTTCCC 
ACGGGAGAGA TCGTGGTCCG CTTGGACCTG CAGTTGTTCG ATGAGCCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010860561.1
CDS	304..5454
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPPLVSSVPAAGVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPTAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVITSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLLTLKRALEAKELARQQLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSIEAERLKQKEQERKILELEKQKEEAQRRAQERDQQWLERMHQEDEQQRPRKPHEEEKLKREESVKKKDSEEKGKPEMQDKLSRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENIKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTEAASTPTPKVAVRETPAPSPATPAEPSETPNNWADFSSTWPTSMNEKPETDSWDAWATQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGPSESPASLKRVASPAAKPAVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKSGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTDPPKPTAFPTVCQVIGMYDYTAQNDDELAFSKGQVINVLNKEDPDWWKGEVHGQVGLFPSNYVKLTTDTDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEGAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILTAQLPHMQPYIRFCSCQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVLCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_010862259.1
Organism	Bison bison bison
Definition	Bison bison bison intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010862259.1

ORF Insert Sequence:

ATGGCTCAGT TTCCCACACC TTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGGTATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCCGTTGTT 
GGGATGTCTC CCCCCCTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT 
GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCA TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACG 
ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT 
GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA 
GAAAACGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC 
AAAGTGGCTG TGCGTGAGAC CCCCGCCCCT TCACCCGCAA CCCCTGCAGA GCCCTCCGAG 
ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCATGAA TGAGAAACCA 
GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG 
CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT 
GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCGC AAGCCCTGTA TCCGTGGAGA 
GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGATG TCATCACCGT ACTGGAGCAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TCCCTCAGAA 
AGTCCTGCAA GTCTAAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCCGT GTCGGGAGAA 
GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GCGATTTAAC CTTTCAGCAA 
GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC 
AAGTCCGGCG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCGGAGGG CTCTGGAACT 
GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATCG CGCAGGTCAT TGCCTCCTAC 
ACAGCTACGG GTCCTGAACA GCTTACCCTG GCCCCCGGTC AGCTGATCCT GATCCGGAAA 
AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTACAGGCAC GTGGGAAAAA GCGCCAGATA 
GGCTGGTTTC CTGCTAATTA CGTCAAACTT TTAAGCCCTG GGACAAGCAA AATCACTCCA 
ACAGACCCAC CGAAGCCAAC AGCTTTCCCA ACAGTGTGCC AGGTGATCGG GATGTACGAC 
TACACCGCGC AGAATGACGA CGAGCTGGCC TTCAGCAAGG GCCAGGTCAT CAATGTCCTC 
AACAAGGAGG ACCCCGACTG GTGGAAGGGT GAAGTCCACG GGCAGGTGGG GCTCTTCCCG 
TCCAACTACG TGAAGTTGAC CACGGACACG GACCCCAGCC AGCAATGGTG CTCAGACTTA 
CACCTCTTGG ATATGCTGAC CCCAACTGAA AGAAAGCGAC AGGGGTACAT CCACGAGCTC 
ATCGTCACGG AGGAGAACTA CGTGAATGAC CTGCAGCTGG TCACAGAGAT CTTTCAGAAA 
CCCCTGATGG AGTCTGAGCT GCTGACAGAA AAAGAGGGTG CTATGATTTT TGTTAACTGG 
AAGGAGCTGA TTATGTGTAA TATCAAACTA CTGAAAGCGC TGAGGGTCCG CAAGAAGATG 
TCTGGGGAGA AGATGCCCGT GAAGATGATC GGAGACATCC TGACGGCCCA GCTGCCACAC 
ATGCAGCCCT ACATCCGCTT CTGCAGCTGC CAGCTCAACG GGGCCGCCTT GATCCAGCAG 
AAGACTGACG AGGCCCCAGA CTTCAAGGAG TTTGTCAAAA GACTGGCCAT GGACCCACGC 
TGTAAGGGAA TGCCCCTGTC TAGTTTTATA CTGAAGCCTA TGCAACGAGT AACGAGATAC 
CCACTGATCA TTAAAAATAT CCTGGAAAAC ACTCCTGAAA ACCACCCAGA CCACAGCCAC 
CTGAAGCACG CCCTGGAGAA GGCGGAGGAG CTGTGCTCCC AGGTGAACGA AGGGGTGCGG 
GAGAAGGAGA ACTCGGACCG ACTGGAGTGG ATCCAGGCCC ACGTGCTGTG TGAAGGCCTG 
TCGGAGCAAC TTGTGTTCAA TTCCGTGACC AACTGCCTGG GGCCGCGCAA GTTCCTACAC 
AGTGGGAAGC TCTACAAGGC CAAGAGCAAC AAGGAGCTGT ACGGCTTCCT CTTCAACGAC 
TTCCTGCTGC TGACCCAAAT CATCAAGCCC CTGGGCTCCT CGGGCACCGA CAAAGTCTTC 
AGCCCCAAAT CAAACTTGCA GTATAAAATG TACAAAACAC CTATTTTCCT AAATGAGGTT 
CTAGTGAAGT TGCCCACTGA CCCTTCTGGG GATGAACCCA TCTTCCACAT TTCCCACATT 
GACCGAGTCT ACACCCTCCG AGCAGAGAGC ATAAACGAAA GGACTGCCTG GGTGCAGAAA 
ATCAAAGCAG CTTCTGAACT CTACATAGAG ACAGAGAAGA AGAAGCGGGA GAAGGCGTAC 
CTGGTCCGCT CCCAAAGAGC CACAGGCATT GGAAGGTTGA TGGTGAACGT GGTTGAAGGC 
ATTGAGCTGA AACCCTGTCG CTCCCATGGA AAGAGTAACC CGTACTGCGA GGTGACCATG 
GGCTCCCAGT GTCACATCAC CAAGACGATC CAGGACACTC TGAATCCCAA GTGGAATTCC 
AACTGCCAGT TCTTCATCAG AGACCTGGAG CAGGAGGTGC TGTGCATCAC TGTGTTTGAG 
AGGGACCAGT TCTCGCCAGA TGATTTTTTG GGTCGGACGG AGATCCGCGT GGCCGACATC 
AAGAAGGACC AGGGCTCCAA GGGTCCCGTT ACGAAGTGTC TCCTGCTGCA CGAAGTTCCC 
ACGGGAGAGA TCGTGGTCCG CTTGGACCTG CAGTTGTTCG ATGAGCCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_010862260.1 ) cDNA ORF clone, Bison bison bison -> XP_010860562.1 Bison bison bison intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBi81218
Clone ID Related Accession (Same CDS sequence)	XM_010862259.1 , XM_010862260.1 , XM_010862261.1
Accession Version	XM_010862260.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	intersectin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011495041.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCCACACC TTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGGTATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCCGTTGTT 
GGGATGTCTC CCCCCCTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT 
GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCA TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACG 
ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT 
GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA 
GAAAACGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC 
AAAGTGGCTG TGCGTGAGAC CCCCGCCCCT TCACCCGCAA CCCCTGCAGA GCCCTCCGAG 
ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCATGAA TGAGAAACCA 
GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG 
CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT 
GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCGC AAGCCCTGTA TCCGTGGAGA 
GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGATG TCATCACCGT ACTGGAGCAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TCCCTCAGAA 
AGTCCTGCAA GTCTAAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCCGT GTCGGGAGAA 
GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GCGATTTAAC CTTTCAGCAA 
GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC 
AAGTCCGGCG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCGGAGGG CTCTGGAACT 
GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATCG CGCAGGTCAT TGCCTCCTAC 
ACAGCTACGG GTCCTGAACA GCTTACCCTG GCCCCCGGTC AGCTGATCCT GATCCGGAAA 
AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTACAGGCAC GTGGGAAAAA GCGCCAGATA 
GGCTGGTTTC CTGCTAATTA CGTCAAACTT TTAAGCCCTG GGACAAGCAA AATCACTCCA 
ACAGACCCAC CGAAGCCAAC AGCTTTCCCA ACAGTGTGCC AGGTGATCGG GATGTACGAC 
TACACCGCGC AGAATGACGA CGAGCTGGCC TTCAGCAAGG GCCAGGTCAT CAATGTCCTC 
AACAAGGAGG ACCCCGACTG GTGGAAGGGT GAAGTCCACG GGCAGGTGGG GCTCTTCCCG 
TCCAACTACG TGAAGTTGAC CACGGACACG GACCCCAGCC AGCAATGGTG CTCAGACTTA 
CACCTCTTGG ATATGCTGAC CCCAACTGAA AGAAAGCGAC AGGGGTACAT CCACGAGCTC 
ATCGTCACGG AGGAGAACTA CGTGAATGAC CTGCAGCTGG TCACAGAGAT CTTTCAGAAA 
CCCCTGATGG AGTCTGAGCT GCTGACAGAA AAAGAGGGTG CTATGATTTT TGTTAACTGG 
AAGGAGCTGA TTATGTGTAA TATCAAACTA CTGAAAGCGC TGAGGGTCCG CAAGAAGATG 
TCTGGGGAGA AGATGCCCGT GAAGATGATC GGAGACATCC TGACGGCCCA GCTGCCACAC 
ATGCAGCCCT ACATCCGCTT CTGCAGCTGC CAGCTCAACG GGGCCGCCTT GATCCAGCAG 
AAGACTGACG AGGCCCCAGA CTTCAAGGAG TTTGTCAAAA GACTGGCCAT GGACCCACGC 
TGTAAGGGAA TGCCCCTGTC TAGTTTTATA CTGAAGCCTA TGCAACGAGT AACGAGATAC 
CCACTGATCA TTAAAAATAT CCTGGAAAAC ACTCCTGAAA ACCACCCAGA CCACAGCCAC 
CTGAAGCACG CCCTGGAGAA GGCGGAGGAG CTGTGCTCCC AGGTGAACGA AGGGGTGCGG 
GAGAAGGAGA ACTCGGACCG ACTGGAGTGG ATCCAGGCCC ACGTGCTGTG TGAAGGCCTG 
TCGGAGCAAC TTGTGTTCAA TTCCGTGACC AACTGCCTGG GGCCGCGCAA GTTCCTACAC 
AGTGGGAAGC TCTACAAGGC CAAGAGCAAC AAGGAGCTGT ACGGCTTCCT CTTCAACGAC 
TTCCTGCTGC TGACCCAAAT CATCAAGCCC CTGGGCTCCT CGGGCACCGA CAAAGTCTTC 
AGCCCCAAAT CAAACTTGCA GTATAAAATG TACAAAACAC CTATTTTCCT AAATGAGGTT 
CTAGTGAAGT TGCCCACTGA CCCTTCTGGG GATGAACCCA TCTTCCACAT TTCCCACATT 
GACCGAGTCT ACACCCTCCG AGCAGAGAGC ATAAACGAAA GGACTGCCTG GGTGCAGAAA 
ATCAAAGCAG CTTCTGAACT CTACATAGAG ACAGAGAAGA AGAAGCGGGA GAAGGCGTAC 
CTGGTCCGCT CCCAAAGAGC CACAGGCATT GGAAGGTTGA TGGTGAACGT GGTTGAAGGC 
ATTGAGCTGA AACCCTGTCG CTCCCATGGA AAGAGTAACC CGTACTGCGA GGTGACCATG 
GGCTCCCAGT GTCACATCAC CAAGACGATC CAGGACACTC TGAATCCCAA GTGGAATTCC 
AACTGCCAGT TCTTCATCAG AGACCTGGAG CAGGAGGTGC TGTGCATCAC TGTGTTTGAG 
AGGGACCAGT TCTCGCCAGA TGATTTTTTG GGTCGGACGG AGATCCGCGT GGCCGACATC 
AAGAAGGACC AGGGCTCCAA GGGTCCCGTT ACGAAGTGTC TCCTGCTGCA CGAAGTTCCC 
ACGGGAGAGA TCGTGGTCCG CTTGGACCTG CAGTTGTTCG ATGAGCCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010860562.1
CDS	575..5725
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPPLVSSVPAAGVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPTAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVITSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLLTLKRALEAKELARQQLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSIEAERLKQKEQERKILELEKQKEEAQRRAQERDQQWLERMHQEDEQQRPRKPHEEEKLKREESVKKKDSEEKGKPEMQDKLSRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENIKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTEAASTPTPKVAVRETPAPSPATPAEPSETPNNWADFSSTWPTSMNEKPETDSWDAWATQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGPSESPASLKRVASPAAKPAVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKSGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTDPPKPTAFPTVCQVIGMYDYTAQNDDELAFSKGQVINVLNKEDPDWWKGEVHGQVGLFPSNYVKLTTDTDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEGAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILTAQLPHMQPYIRFCSCQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVLCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_010862260.1
Organism	Bison bison bison
Definition	Bison bison bison intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010862260.1

ORF Insert Sequence:

ATGGCTCAGT TTCCCACACC TTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGGTATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCCGTTGTT 
GGGATGTCTC CCCCCCTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT 
GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCA TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACG 
ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT 
GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA 
GAAAACGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC 
AAAGTGGCTG TGCGTGAGAC CCCCGCCCCT TCACCCGCAA CCCCTGCAGA GCCCTCCGAG 
ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCATGAA TGAGAAACCA 
GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG 
CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT 
GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCGC AAGCCCTGTA TCCGTGGAGA 
GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGATG TCATCACCGT ACTGGAGCAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TCCCTCAGAA 
AGTCCTGCAA GTCTAAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCCGT GTCGGGAGAA 
GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GCGATTTAAC CTTTCAGCAA 
GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC 
AAGTCCGGCG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCGGAGGG CTCTGGAACT 
GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATCG CGCAGGTCAT TGCCTCCTAC 
ACAGCTACGG GTCCTGAACA GCTTACCCTG GCCCCCGGTC AGCTGATCCT GATCCGGAAA 
AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTACAGGCAC GTGGGAAAAA GCGCCAGATA 
GGCTGGTTTC CTGCTAATTA CGTCAAACTT TTAAGCCCTG GGACAAGCAA AATCACTCCA 
ACAGACCCAC CGAAGCCAAC AGCTTTCCCA ACAGTGTGCC AGGTGATCGG GATGTACGAC 
TACACCGCGC AGAATGACGA CGAGCTGGCC TTCAGCAAGG GCCAGGTCAT CAATGTCCTC 
AACAAGGAGG ACCCCGACTG GTGGAAGGGT GAAGTCCACG GGCAGGTGGG GCTCTTCCCG 
TCCAACTACG TGAAGTTGAC CACGGACACG GACCCCAGCC AGCAATGGTG CTCAGACTTA 
CACCTCTTGG ATATGCTGAC CCCAACTGAA AGAAAGCGAC AGGGGTACAT CCACGAGCTC 
ATCGTCACGG AGGAGAACTA CGTGAATGAC CTGCAGCTGG TCACAGAGAT CTTTCAGAAA 
CCCCTGATGG AGTCTGAGCT GCTGACAGAA AAAGAGGGTG CTATGATTTT TGTTAACTGG 
AAGGAGCTGA TTATGTGTAA TATCAAACTA CTGAAAGCGC TGAGGGTCCG CAAGAAGATG 
TCTGGGGAGA AGATGCCCGT GAAGATGATC GGAGACATCC TGACGGCCCA GCTGCCACAC 
ATGCAGCCCT ACATCCGCTT CTGCAGCTGC CAGCTCAACG GGGCCGCCTT GATCCAGCAG 
AAGACTGACG AGGCCCCAGA CTTCAAGGAG TTTGTCAAAA GACTGGCCAT GGACCCACGC 
TGTAAGGGAA TGCCCCTGTC TAGTTTTATA CTGAAGCCTA TGCAACGAGT AACGAGATAC 
CCACTGATCA TTAAAAATAT CCTGGAAAAC ACTCCTGAAA ACCACCCAGA CCACAGCCAC 
CTGAAGCACG CCCTGGAGAA GGCGGAGGAG CTGTGCTCCC AGGTGAACGA AGGGGTGCGG 
GAGAAGGAGA ACTCGGACCG ACTGGAGTGG ATCCAGGCCC ACGTGCTGTG TGAAGGCCTG 
TCGGAGCAAC TTGTGTTCAA TTCCGTGACC AACTGCCTGG GGCCGCGCAA GTTCCTACAC 
AGTGGGAAGC TCTACAAGGC CAAGAGCAAC AAGGAGCTGT ACGGCTTCCT CTTCAACGAC 
TTCCTGCTGC TGACCCAAAT CATCAAGCCC CTGGGCTCCT CGGGCACCGA CAAAGTCTTC 
AGCCCCAAAT CAAACTTGCA GTATAAAATG TACAAAACAC CTATTTTCCT AAATGAGGTT 
CTAGTGAAGT TGCCCACTGA CCCTTCTGGG GATGAACCCA TCTTCCACAT TTCCCACATT 
GACCGAGTCT ACACCCTCCG AGCAGAGAGC ATAAACGAAA GGACTGCCTG GGTGCAGAAA 
ATCAAAGCAG CTTCTGAACT CTACATAGAG ACAGAGAAGA AGAAGCGGGA GAAGGCGTAC 
CTGGTCCGCT CCCAAAGAGC CACAGGCATT GGAAGGTTGA TGGTGAACGT GGTTGAAGGC 
ATTGAGCTGA AACCCTGTCG CTCCCATGGA AAGAGTAACC CGTACTGCGA GGTGACCATG 
GGCTCCCAGT GTCACATCAC CAAGACGATC CAGGACACTC TGAATCCCAA GTGGAATTCC 
AACTGCCAGT TCTTCATCAG AGACCTGGAG CAGGAGGTGC TGTGCATCAC TGTGTTTGAG 
AGGGACCAGT TCTCGCCAGA TGATTTTTTG GGTCGGACGG AGATCCGCGT GGCCGACATC 
AAGAAGGACC AGGGCTCCAA GGGTCCCGTT ACGAAGTGTC TCCTGCTGCA CGAAGTTCCC 
ACGGGAGAGA TCGTGGTCCG CTTGGACCTG CAGTTGTTCG ATGAGCCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_010862261.1 ) cDNA ORF clone, Bison bison bison -> XP_010860563.1 Bison bison bison intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X3, mRNA.

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CloneID	OBi81218
Clone ID Related Accession (Same CDS sequence)	XM_010862259.1 , XM_010862260.1 , XM_010862261.1
Accession Version	XM_010862261.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	intersectin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011495041.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCCACACC TTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGGTATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCCGTTGTT 
GGGATGTCTC CCCCCCTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT 
GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCA TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACG 
ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT 
GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA 
GAAAACGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC 
AAAGTGGCTG TGCGTGAGAC CCCCGCCCCT TCACCCGCAA CCCCTGCAGA GCCCTCCGAG 
ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCATGAA TGAGAAACCA 
GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG 
CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT 
GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCGC AAGCCCTGTA TCCGTGGAGA 
GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGATG TCATCACCGT ACTGGAGCAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TCCCTCAGAA 
AGTCCTGCAA GTCTAAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCCGT GTCGGGAGAA 
GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GCGATTTAAC CTTTCAGCAA 
GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC 
AAGTCCGGCG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCGGAGGG CTCTGGAACT 
GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATCG CGCAGGTCAT TGCCTCCTAC 
ACAGCTACGG GTCCTGAACA GCTTACCCTG GCCCCCGGTC AGCTGATCCT GATCCGGAAA 
AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTACAGGCAC GTGGGAAAAA GCGCCAGATA 
GGCTGGTTTC CTGCTAATTA CGTCAAACTT TTAAGCCCTG GGACAAGCAA AATCACTCCA 
ACAGACCCAC CGAAGCCAAC AGCTTTCCCA ACAGTGTGCC AGGTGATCGG GATGTACGAC 
TACACCGCGC AGAATGACGA CGAGCTGGCC TTCAGCAAGG GCCAGGTCAT CAATGTCCTC 
AACAAGGAGG ACCCCGACTG GTGGAAGGGT GAAGTCCACG GGCAGGTGGG GCTCTTCCCG 
TCCAACTACG TGAAGTTGAC CACGGACACG GACCCCAGCC AGCAATGGTG CTCAGACTTA 
CACCTCTTGG ATATGCTGAC CCCAACTGAA AGAAAGCGAC AGGGGTACAT CCACGAGCTC 
ATCGTCACGG AGGAGAACTA CGTGAATGAC CTGCAGCTGG TCACAGAGAT CTTTCAGAAA 
CCCCTGATGG AGTCTGAGCT GCTGACAGAA AAAGAGGGTG CTATGATTTT TGTTAACTGG 
AAGGAGCTGA TTATGTGTAA TATCAAACTA CTGAAAGCGC TGAGGGTCCG CAAGAAGATG 
TCTGGGGAGA AGATGCCCGT GAAGATGATC GGAGACATCC TGACGGCCCA GCTGCCACAC 
ATGCAGCCCT ACATCCGCTT CTGCAGCTGC CAGCTCAACG GGGCCGCCTT GATCCAGCAG 
AAGACTGACG AGGCCCCAGA CTTCAAGGAG TTTGTCAAAA GACTGGCCAT GGACCCACGC 
TGTAAGGGAA TGCCCCTGTC TAGTTTTATA CTGAAGCCTA TGCAACGAGT AACGAGATAC 
CCACTGATCA TTAAAAATAT CCTGGAAAAC ACTCCTGAAA ACCACCCAGA CCACAGCCAC 
CTGAAGCACG CCCTGGAGAA GGCGGAGGAG CTGTGCTCCC AGGTGAACGA AGGGGTGCGG 
GAGAAGGAGA ACTCGGACCG ACTGGAGTGG ATCCAGGCCC ACGTGCTGTG TGAAGGCCTG 
TCGGAGCAAC TTGTGTTCAA TTCCGTGACC AACTGCCTGG GGCCGCGCAA GTTCCTACAC 
AGTGGGAAGC TCTACAAGGC CAAGAGCAAC AAGGAGCTGT ACGGCTTCCT CTTCAACGAC 
TTCCTGCTGC TGACCCAAAT CATCAAGCCC CTGGGCTCCT CGGGCACCGA CAAAGTCTTC 
AGCCCCAAAT CAAACTTGCA GTATAAAATG TACAAAACAC CTATTTTCCT AAATGAGGTT 
CTAGTGAAGT TGCCCACTGA CCCTTCTGGG GATGAACCCA TCTTCCACAT TTCCCACATT 
GACCGAGTCT ACACCCTCCG AGCAGAGAGC ATAAACGAAA GGACTGCCTG GGTGCAGAAA 
ATCAAAGCAG CTTCTGAACT CTACATAGAG ACAGAGAAGA AGAAGCGGGA GAAGGCGTAC 
CTGGTCCGCT CCCAAAGAGC CACAGGCATT GGAAGGTTGA TGGTGAACGT GGTTGAAGGC 
ATTGAGCTGA AACCCTGTCG CTCCCATGGA AAGAGTAACC CGTACTGCGA GGTGACCATG 
GGCTCCCAGT GTCACATCAC CAAGACGATC CAGGACACTC TGAATCCCAA GTGGAATTCC 
AACTGCCAGT TCTTCATCAG AGACCTGGAG CAGGAGGTGC TGTGCATCAC TGTGTTTGAG 
AGGGACCAGT TCTCGCCAGA TGATTTTTTG GGTCGGACGG AGATCCGCGT GGCCGACATC 
AAGAAGGACC AGGGCTCCAA GGGTCCCGTT ACGAAGTGTC TCCTGCTGCA CGAAGTTCCC 
ACGGGAGAGA TCGTGGTCCG CTTGGACCTG CAGTTGTTCG ATGAGCCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010860563.1
CDS	2131..7281
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPPLVSSVPAAGVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPTAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVITSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLLTLKRALEAKELARQQLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSIEAERLKQKEQERKILELEKQKEEAQRRAQERDQQWLERMHQEDEQQRPRKPHEEEKLKREESVKKKDSEEKGKPEMQDKLSRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENIKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTEAASTPTPKVAVRETPAPSPATPAEPSETPNNWADFSSTWPTSMNEKPETDSWDAWATQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGPSESPASLKRVASPAAKPAVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKSGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTDPPKPTAFPTVCQVIGMYDYTAQNDDELAFSKGQVINVLNKEDPDWWKGEVHGQVGLFPSNYVKLTTDTDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEGAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILTAQLPHMQPYIRFCSCQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVLCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_010862261.1
Organism	Bison bison bison
Definition	Bison bison bison intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010862261.1

ORF Insert Sequence:

ATGGCTCAGT TTCCCACACC TTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGGTATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCCGTTGTT 
GGGATGTCTC CCCCCCTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT 
GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCA TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACG 
ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT 
GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA 
GAAAACGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC 
AAAGTGGCTG TGCGTGAGAC CCCCGCCCCT TCACCCGCAA CCCCTGCAGA GCCCTCCGAG 
ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCATGAA TGAGAAACCA 
GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG 
CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT 
GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCGC AAGCCCTGTA TCCGTGGAGA 
GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGATG TCATCACCGT ACTGGAGCAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TCCCTCAGAA 
AGTCCTGCAA GTCTAAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCCGT GTCGGGAGAA 
GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GCGATTTAAC CTTTCAGCAA 
GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC 
AAGTCCGGCG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCGGAGGG CTCTGGAACT 
GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATCG CGCAGGTCAT TGCCTCCTAC 
ACAGCTACGG GTCCTGAACA GCTTACCCTG GCCCCCGGTC AGCTGATCCT GATCCGGAAA 
AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTACAGGCAC GTGGGAAAAA GCGCCAGATA 
GGCTGGTTTC CTGCTAATTA CGTCAAACTT TTAAGCCCTG GGACAAGCAA AATCACTCCA 
ACAGACCCAC CGAAGCCAAC AGCTTTCCCA ACAGTGTGCC AGGTGATCGG GATGTACGAC 
TACACCGCGC AGAATGACGA CGAGCTGGCC TTCAGCAAGG GCCAGGTCAT CAATGTCCTC 
AACAAGGAGG ACCCCGACTG GTGGAAGGGT GAAGTCCACG GGCAGGTGGG GCTCTTCCCG 
TCCAACTACG TGAAGTTGAC CACGGACACG GACCCCAGCC AGCAATGGTG CTCAGACTTA 
CACCTCTTGG ATATGCTGAC CCCAACTGAA AGAAAGCGAC AGGGGTACAT CCACGAGCTC 
ATCGTCACGG AGGAGAACTA CGTGAATGAC CTGCAGCTGG TCACAGAGAT CTTTCAGAAA 
CCCCTGATGG AGTCTGAGCT GCTGACAGAA AAAGAGGGTG CTATGATTTT TGTTAACTGG 
AAGGAGCTGA TTATGTGTAA TATCAAACTA CTGAAAGCGC TGAGGGTCCG CAAGAAGATG 
TCTGGGGAGA AGATGCCCGT GAAGATGATC GGAGACATCC TGACGGCCCA GCTGCCACAC 
ATGCAGCCCT ACATCCGCTT CTGCAGCTGC CAGCTCAACG GGGCCGCCTT GATCCAGCAG 
AAGACTGACG AGGCCCCAGA CTTCAAGGAG TTTGTCAAAA GACTGGCCAT GGACCCACGC 
TGTAAGGGAA TGCCCCTGTC TAGTTTTATA CTGAAGCCTA TGCAACGAGT AACGAGATAC 
CCACTGATCA TTAAAAATAT CCTGGAAAAC ACTCCTGAAA ACCACCCAGA CCACAGCCAC 
CTGAAGCACG CCCTGGAGAA GGCGGAGGAG CTGTGCTCCC AGGTGAACGA AGGGGTGCGG 
GAGAAGGAGA ACTCGGACCG ACTGGAGTGG ATCCAGGCCC ACGTGCTGTG TGAAGGCCTG 
TCGGAGCAAC TTGTGTTCAA TTCCGTGACC AACTGCCTGG GGCCGCGCAA GTTCCTACAC 
AGTGGGAAGC TCTACAAGGC CAAGAGCAAC AAGGAGCTGT ACGGCTTCCT CTTCAACGAC 
TTCCTGCTGC TGACCCAAAT CATCAAGCCC CTGGGCTCCT CGGGCACCGA CAAAGTCTTC 
AGCCCCAAAT CAAACTTGCA GTATAAAATG TACAAAACAC CTATTTTCCT AAATGAGGTT 
CTAGTGAAGT TGCCCACTGA CCCTTCTGGG GATGAACCCA TCTTCCACAT TTCCCACATT 
GACCGAGTCT ACACCCTCCG AGCAGAGAGC ATAAACGAAA GGACTGCCTG GGTGCAGAAA 
ATCAAAGCAG CTTCTGAACT CTACATAGAG ACAGAGAAGA AGAAGCGGGA GAAGGCGTAC 
CTGGTCCGCT CCCAAAGAGC CACAGGCATT GGAAGGTTGA TGGTGAACGT GGTTGAAGGC 
ATTGAGCTGA AACCCTGTCG CTCCCATGGA AAGAGTAACC CGTACTGCGA GGTGACCATG 
GGCTCCCAGT GTCACATCAC CAAGACGATC CAGGACACTC TGAATCCCAA GTGGAATTCC 
AACTGCCAGT TCTTCATCAG AGACCTGGAG CAGGAGGTGC TGTGCATCAC TGTGTTTGAG 
AGGGACCAGT TCTCGCCAGA TGATTTTTTG GGTCGGACGG AGATCCGCGT GGCCGACATC 
AAGAAGGACC AGGGCTCCAA GGGTCCCGTT ACGAAGTGTC TCCTGCTGCA CGAAGTTCCC 
ACGGGAGAGA TCGTGGTCCG CTTGGACCTG CAGTTGTTCG ATGAGCCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_010862262.1 ) cDNA ORF clone, Bison bison bison -> XP_010860564.1 Bison bison bison intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X4, mRNA.

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CloneID	OBi81219
Clone ID Related Accession (Same CDS sequence)	XM_010862262.1
Accession Version	XM_010862262.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	intersectin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011495041.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCCACACC TTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGGTATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCCGTTGTT 
GGGATGTCTC CCCCCCTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT 
GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCA TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACG 
ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT 
GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA 
GAAAACGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC 
AAAGTGGCTG TGCGTGAGAC CCCCGCCCCT TCACCCGCAA CCCCTGCAGA GCCCTCCGAG 
ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCATGAA TGAGAAACCA 
GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG 
CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT 
GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCGC AAGCCCTGTA TCCGTGGAGA 
GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGATG TCATCACCGT ACTGGAGCAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TCCCTCAGAA 
AGTCCTGCAA GTCTAAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCCGT GTCGGGAGAA 
GAAATCGCGC AGGTCATTGC CTCCTACACA GCTACGGGTC CTGAACAGCT TACCCTGGCC 
CCCGGTCAGC TGATCCTGAT CCGGAAAAAG AACCCAGGTG GATGGTGGGA AGGAGAGCTA 
CAGGCACGTG GGAAAAAGCG CCAGATAGGC TGGTTTCCTG CTAATTACGT CAAACTTTTA 
AGCCCTGGGA CAAGCAAAAT CACTCCAACA GACCCACCGA AGCCAACAGC TTTCCCAACA 
GTGTGCCAGG TGATCGGGAT GTACGACTAC ACCGCGCAGA ATGACGACGA GCTGGCCTTC 
AGCAAGGGCC AGGTCATCAA TGTCCTCAAC AAGGAGGACC CCGACTGGTG GAAGGGTGAA 
GTCCACGGGC AGGTGGGGCT CTTCCCGTCC AACTACGTGA AGTTGACCAC GGACACGGAC 
CCCAGCCAGC AATGGTGCTC AGACTTACAC CTCTTGGATA TGCTGACCCC AACTGAAAGA 
AAGCGACAGG GGTACATCCA CGAGCTCATC GTCACGGAGG AGAACTACGT GAATGACCTG 
CAGCTGGTCA CAGAGATCTT TCAGAAACCC CTGATGGAGT CTGAGCTGCT GACAGAAAAA 
GAGGGTGCTA TGATTTTTGT TAACTGGAAG GAGCTGATTA TGTGTAATAT CAAACTACTG 
AAAGCGCTGA GGGTCCGCAA GAAGATGTCT GGGGAGAAGA TGCCCGTGAA GATGATCGGA 
GACATCCTGA CGGCCCAGCT GCCACACATG CAGCCCTACA TCCGCTTCTG CAGCTGCCAG 
CTCAACGGGG CCGCCTTGAT CCAGCAGAAG ACTGACGAGG CCCCAGACTT CAAGGAGTTT 
GTCAAAAGAC TGGCCATGGA CCCACGCTGT AAGGGAATGC CCCTGTCTAG TTTTATACTG 
AAGCCTATGC AACGAGTAAC GAGATACCCA CTGATCATTA AAAATATCCT GGAAAACACT 
CCTGAAAACC ACCCAGACCA CAGCCACCTG AAGCACGCCC TGGAGAAGGC GGAGGAGCTG 
TGCTCCCAGG TGAACGAAGG GGTGCGGGAG AAGGAGAACT CGGACCGACT GGAGTGGATC 
CAGGCCCACG TGCTGTGTGA AGGCCTGTCG GAGCAACTTG TGTTCAATTC CGTGACCAAC 
TGCCTGGGGC CGCGCAAGTT CCTACACAGT GGGAAGCTCT ACAAGGCCAA GAGCAACAAG 
GAGCTGTACG GCTTCCTCTT CAACGACTTC CTGCTGCTGA CCCAAATCAT CAAGCCCCTG 
GGCTCCTCGG GCACCGACAA AGTCTTCAGC CCCAAATCAA ACTTGCAGTA TAAAATGTAC 
AAAACACCTA TTTTCCTAAA TGAGGTTCTA GTGAAGTTGC CCACTGACCC TTCTGGGGAT 
GAACCCATCT TCCACATTTC CCACATTGAC CGAGTCTACA CCCTCCGAGC AGAGAGCATA 
AACGAAAGGA CTGCCTGGGT GCAGAAAATC AAAGCAGCTT CTGAACTCTA CATAGAGACA 
GAGAAGAAGA AGCGGGAGAA GGCGTACCTG GTCCGCTCCC AAAGAGCCAC AGGCATTGGA 
AGGTTGATGG TGAACGTGGT TGAAGGCATT GAGCTGAAAC CCTGTCGCTC CCATGGAAAG 
AGTAACCCGT ACTGCGAGGT GACCATGGGC TCCCAGTGTC ACATCACCAA GACGATCCAG 
GACACTCTGA ATCCCAAGTG GAATTCCAAC TGCCAGTTCT TCATCAGAGA CCTGGAGCAG 
GAGGTGCTGT GCATCACTGT GTTTGAGAGG GACCAGTTCT CGCCAGATGA TTTTTTGGGT 
CGGACGGAGA TCCGCGTGGC CGACATCAAG AAGGACCAGG GCTCCAAGGG TCCCGTTACG 
AAGTGTCTCC TGCTGCACGA AGTTCCCACG GGAGAGATCG TGGTCCGCTT GGACCTGCAG 
TTGTTCGATG AGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010860564.1
CDS	304..5241
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPPLVSSVPAAGVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPTAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVITSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLLTLKRALEAKELARQQLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSIEAERLKQKEQERKILELEKQKEEAQRRAQERDQQWLERMHQEDEQQRPRKPHEEEKLKREESVKKKDSEEKGKPEMQDKLSRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENIKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTEAASTPTPKVAVRETPAPSPATPAEPSETPNNWADFSSTWPTSMNEKPETDSWDAWATQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGPSESPASLKRVASPAAKPAVSGEEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTDPPKPTAFPTVCQVIGMYDYTAQNDDELAFSKGQVINVLNKEDPDWWKGEVHGQVGLFPSNYVKLTTDTDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEGAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILTAQLPHMQPYIRFCSCQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVLCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_010862262.1
Organism	Bison bison bison
Definition	Bison bison bison intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010862262.1

ORF Insert Sequence:

ATGGCTCAGT TTCCCACACC TTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGGTATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCCGTTGTT 
GGGATGTCTC CCCCCCTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT 
GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCA TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACG 
ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT 
GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA 
GAAAACGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC 
AAAGTGGCTG TGCGTGAGAC CCCCGCCCCT TCACCCGCAA CCCCTGCAGA GCCCTCCGAG 
ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCATGAA TGAGAAACCA 
GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG 
CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT 
GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCGC AAGCCCTGTA TCCGTGGAGA 
GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGATG TCATCACCGT ACTGGAGCAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TCCCTCAGAA 
AGTCCTGCAA GTCTAAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCCGT GTCGGGAGAA 
GAAATCGCGC AGGTCATTGC CTCCTACACA GCTACGGGTC CTGAACAGCT TACCCTGGCC 
CCCGGTCAGC TGATCCTGAT CCGGAAAAAG AACCCAGGTG GATGGTGGGA AGGAGAGCTA 
CAGGCACGTG GGAAAAAGCG CCAGATAGGC TGGTTTCCTG CTAATTACGT CAAACTTTTA 
AGCCCTGGGA CAAGCAAAAT CACTCCAACA GACCCACCGA AGCCAACAGC TTTCCCAACA 
GTGTGCCAGG TGATCGGGAT GTACGACTAC ACCGCGCAGA ATGACGACGA GCTGGCCTTC 
AGCAAGGGCC AGGTCATCAA TGTCCTCAAC AAGGAGGACC CCGACTGGTG GAAGGGTGAA 
GTCCACGGGC AGGTGGGGCT CTTCCCGTCC AACTACGTGA AGTTGACCAC GGACACGGAC 
CCCAGCCAGC AATGGTGCTC AGACTTACAC CTCTTGGATA TGCTGACCCC AACTGAAAGA 
AAGCGACAGG GGTACATCCA CGAGCTCATC GTCACGGAGG AGAACTACGT GAATGACCTG 
CAGCTGGTCA CAGAGATCTT TCAGAAACCC CTGATGGAGT CTGAGCTGCT GACAGAAAAA 
GAGGGTGCTA TGATTTTTGT TAACTGGAAG GAGCTGATTA TGTGTAATAT CAAACTACTG 
AAAGCGCTGA GGGTCCGCAA GAAGATGTCT GGGGAGAAGA TGCCCGTGAA GATGATCGGA 
GACATCCTGA CGGCCCAGCT GCCACACATG CAGCCCTACA TCCGCTTCTG CAGCTGCCAG 
CTCAACGGGG CCGCCTTGAT CCAGCAGAAG ACTGACGAGG CCCCAGACTT CAAGGAGTTT 
GTCAAAAGAC TGGCCATGGA CCCACGCTGT AAGGGAATGC CCCTGTCTAG TTTTATACTG 
AAGCCTATGC AACGAGTAAC GAGATACCCA CTGATCATTA AAAATATCCT GGAAAACACT 
CCTGAAAACC ACCCAGACCA CAGCCACCTG AAGCACGCCC TGGAGAAGGC GGAGGAGCTG 
TGCTCCCAGG TGAACGAAGG GGTGCGGGAG AAGGAGAACT CGGACCGACT GGAGTGGATC 
CAGGCCCACG TGCTGTGTGA AGGCCTGTCG GAGCAACTTG TGTTCAATTC CGTGACCAAC 
TGCCTGGGGC CGCGCAAGTT CCTACACAGT GGGAAGCTCT ACAAGGCCAA GAGCAACAAG 
GAGCTGTACG GCTTCCTCTT CAACGACTTC CTGCTGCTGA CCCAAATCAT CAAGCCCCTG 
GGCTCCTCGG GCACCGACAA AGTCTTCAGC CCCAAATCAA ACTTGCAGTA TAAAATGTAC 
AAAACACCTA TTTTCCTAAA TGAGGTTCTA GTGAAGTTGC CCACTGACCC TTCTGGGGAT 
GAACCCATCT TCCACATTTC CCACATTGAC CGAGTCTACA CCCTCCGAGC AGAGAGCATA 
AACGAAAGGA CTGCCTGGGT GCAGAAAATC AAAGCAGCTT CTGAACTCTA CATAGAGACA 
GAGAAGAAGA AGCGGGAGAA GGCGTACCTG GTCCGCTCCC AAAGAGCCAC AGGCATTGGA 
AGGTTGATGG TGAACGTGGT TGAAGGCATT GAGCTGAAAC CCTGTCGCTC CCATGGAAAG 
AGTAACCCGT ACTGCGAGGT GACCATGGGC TCCCAGTGTC ACATCACCAA GACGATCCAG 
GACACTCTGA ATCCCAAGTG GAATTCCAAC TGCCAGTTCT TCATCAGAGA CCTGGAGCAG 
GAGGTGCTGT GCATCACTGT GTTTGAGAGG GACCAGTTCT CGCCAGATGA TTTTTTGGGT 
CGGACGGAGA TCCGCGTGGC CGACATCAAG AAGGACCAGG GCTCCAAGGG TCCCGTTACG 
AAGTGTCTCC TGCTGCACGA AGTTCCCACG GGAGAGATCG TGGTCCGCTT GGACCTGCAG 
TTGTTCGATG AGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_010862263.1 ) cDNA ORF clone, Bison bison bison -> XP_010860565.1 Bison bison bison intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X5, mRNA.

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CloneID	OBi81220
Clone ID Related Accession (Same CDS sequence)	XM_010862263.1
Accession Version	XM_010862263.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	intersectin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011495041.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCCACACC TTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGGTATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCCGTTGTT 
GGGATGTCTC CCCCCCTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT 
GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCA TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACG 
ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT 
GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA 
GAAAACGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC 
AAAGTGGCTG TGCGTGAGAC CCCCGCCCCT TCACCCGCAA CCCCTGCAGA GCCCTCCGAG 
ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCATGAA TGAGAAACCA 
GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG 
CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT 
GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCGC AAGCCCTGTA TCCGTGGAGA 
GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGATG TCATCACCGT ACTGGAGCAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TCCCTCAGAA 
AGTCCTGCAA GTCTAAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCCGT GTCGGGAGAA 
GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GCGATTTAAC CTTTCAGCAA 
GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC 
AAGTCCGGCG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCGGAGGG CTCTGGAACT 
GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATCG CGCAGGTCAT TGCCTCCTAC 
ACAGCTACGG GTCCTGAACA GCTTACCCTG GCCCCCGGTC AGCTGATCCT GATCCGGAAA 
AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTACAGGCAC GTGGGAAAAA GCGCCAGATA 
GGCTGGTTTC CTGCTAATTA CGTCAAACTT TTAAGCCCTG GGACAAGCAA AATCACTCCA 
ACAGACCCAC CGAAGCCAAC AGCTTTCCCA ACAGTGTGCC AGGTGATCGG GATGTACGAC 
TACACCGCGC AGAATGACGA CGAGCTGGCC TTCAGCAAGG GCCAGGTCAT CAATGTCCTC 
AACAAGGAGG ACCCCGACTG GTGGAAGGGT GAAGTCCACG GGCAGGTGGG GCTCTTCCCG 
TCCAACTACG TGAAGTTGAC CACGGACACG GACCCCAGCC AGCAATGGTG CTCAGACTTA 
CACCTCTTGG ATATGCTGAC CCCAACTGAA AGAAAGCGAC AGGGGTACAT CCACGAGCTC 
ATCGTCACGG AGGAGAACTA CGTGAATGAC CTGCAGCTGG TCACAGAGAG ACTGGCCATG 
GACCCACGCT GTAAGGGAAT GCCCCTGTCT AGTTTTATAC TGAAGCCTAT GCAACGAGTA 
ACGAGATACC CACTGATCAT TAAAAATATC CTGGAAAACA CTCCTGAAAA CCACCCAGAC 
CACAGCCACC TGAAGCACGC CCTGGAGAAG GCGGAGGAGC TGTGCTCCCA GGTGAACGAA 
GGGGTGCGGG AGAAGGAGAA CTCGGACCGA CTGGAGTGGA TCCAGGCCCA CGTGCTGTGT 
GAAGGCCTGT CGGAGCAACT TGTGTTCAAT TCCGTGACCA ACTGCCTGGG GCCGCGCAAG 
TTCCTACACA GTGGGAAGCT CTACAAGGCC AAGAGCAACA AGGAGCTGTA CGGCTTCCTC 
TTCAACGACT TCCTGCTGCT GACCCAAATC ATCAAGCCCC TGGGCTCCTC GGGCACCGAC 
AAAGTCTTCA GCCCCAAATC AAACTTGCAG TATAAAATGT ACAAAACACC TATTTTCCTA 
AATGAGGTTC TAGTGAAGTT GCCCACTGAC CCTTCTGGGG ATGAACCCAT CTTCCACATT 
TCCCACATTG ACCGAGTCTA CACCCTCCGA GCAGAGAGCA TAAACGAAAG GACTGCCTGG 
GTGCAGAAAA TCAAAGCAGC TTCTGAACTC TACATAGAGA CAGAGAAGAA GAAGCGGGAG 
AAGGCGTACC TGGTCCGCTC CCAAAGAGCC ACAGGCATTG GAAGGTTGAT GGTGAACGTG 
GTTGAAGGCA TTGAGCTGAA ACCCTGTCGC TCCCATGGAA AGAGTAACCC GTACTGCGAG 
GTGACCATGG GCTCCCAGTG TCACATCACC AAGACGATCC AGGACACTCT GAATCCCAAG 
TGGAATTCCA ACTGCCAGTT CTTCATCAGA GACCTGGAGC AGGAGGTGCT GTGCATCACT 
GTGTTTGAGA GGGACCAGTT CTCGCCAGAT GATTTTTTGG GTCGGACGGA GATCCGCGTG 
GCCGACATCA AGAAGGACCA GGGCTCCAAG GGTCCCGTTA CGAAGTGTCT CCTGCTGCAC 
GAAGTTCCCA CGGGAGAGAT CGTGGTCCGC TTGGACCTGC AGTTGTTCGA TGAGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010860565.1
CDS	304..5163
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPPLVSSVPAAGVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPTAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVITSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLLTLKRALEAKELARQQLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSIEAERLKQKEQERKILELEKQKEEAQRRAQERDQQWLERMHQEDEQQRPRKPHEEEKLKREESVKKKDSEEKGKPEMQDKLSRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENIKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTEAASTPTPKVAVRETPAPSPATPAEPSETPNNWADFSSTWPTSMNEKPETDSWDAWATQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGPSESPASLKRVASPAAKPAVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKSGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTDPPKPTAFPTVCQVIGMYDYTAQNDDELAFSKGQVINVLNKEDPDWWKGEVHGQVGLFPSNYVKLTTDTDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTERLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVLCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_010862263.1
Organism	Bison bison bison
Definition	Bison bison bison intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010862263.1

ORF Insert Sequence:

ATGGCTCAGT TTCCCACACC TTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG 
ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT 
GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGGTATCAGC TCCCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT 
GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTGCCAA TGGGCTCCAT TCCCGTTGTT 
GGGATGTCTC CCCCCCTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT 
GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG 
GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA 
TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC 
CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC 
CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA 
ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA 
CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG 
GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG 
CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG 
CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG 
CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA 
AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA 
TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG 
CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG 
CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT 
GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG 
AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG 
CTGGAGCGCA TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG 
AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA 
ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA 
AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACG 
ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT 
GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA 
GAAAACGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC 
AAAGTGGCTG TGCGTGAGAC CCCCGCCCCT TCACCCGCAA CCCCTGCAGA GCCCTCCGAG 
ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCATGAA TGAGAAACCA 
GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG 
CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT 
GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCGC AAGCCCTGTA TCCGTGGAGA 
GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGATG TCATCACCGT ACTGGAGCAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TCCCTCAGAA 
AGTCCTGCAA GTCTAAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCCGT GTCGGGAGAA 
GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GCGATTTAAC CTTTCAGCAA 
GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC 
AAGTCCGGCG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCGGAGGG CTCTGGAACT 
GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATCG CGCAGGTCAT TGCCTCCTAC 
ACAGCTACGG GTCCTGAACA GCTTACCCTG GCCCCCGGTC AGCTGATCCT GATCCGGAAA 
AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTACAGGCAC GTGGGAAAAA GCGCCAGATA 
GGCTGGTTTC CTGCTAATTA CGTCAAACTT TTAAGCCCTG GGACAAGCAA AATCACTCCA 
ACAGACCCAC CGAAGCCAAC AGCTTTCCCA ACAGTGTGCC AGGTGATCGG GATGTACGAC 
TACACCGCGC AGAATGACGA CGAGCTGGCC TTCAGCAAGG GCCAGGTCAT CAATGTCCTC 
AACAAGGAGG ACCCCGACTG GTGGAAGGGT GAAGTCCACG GGCAGGTGGG GCTCTTCCCG 
TCCAACTACG TGAAGTTGAC CACGGACACG GACCCCAGCC AGCAATGGTG CTCAGACTTA 
CACCTCTTGG ATATGCTGAC CCCAACTGAA AGAAAGCGAC AGGGGTACAT CCACGAGCTC 
ATCGTCACGG AGGAGAACTA CGTGAATGAC CTGCAGCTGG TCACAGAGAG ACTGGCCATG 
GACCCACGCT GTAAGGGAAT GCCCCTGTCT AGTTTTATAC TGAAGCCTAT GCAACGAGTA 
ACGAGATACC CACTGATCAT TAAAAATATC CTGGAAAACA CTCCTGAAAA CCACCCAGAC 
CACAGCCACC TGAAGCACGC CCTGGAGAAG GCGGAGGAGC TGTGCTCCCA GGTGAACGAA 
GGGGTGCGGG AGAAGGAGAA CTCGGACCGA CTGGAGTGGA TCCAGGCCCA CGTGCTGTGT 
GAAGGCCTGT CGGAGCAACT TGTGTTCAAT TCCGTGACCA ACTGCCTGGG GCCGCGCAAG 
TTCCTACACA GTGGGAAGCT CTACAAGGCC AAGAGCAACA AGGAGCTGTA CGGCTTCCTC 
TTCAACGACT TCCTGCTGCT GACCCAAATC ATCAAGCCCC TGGGCTCCTC GGGCACCGAC 
AAAGTCTTCA GCCCCAAATC AAACTTGCAG TATAAAATGT ACAAAACACC TATTTTCCTA 
AATGAGGTTC TAGTGAAGTT GCCCACTGAC CCTTCTGGGG ATGAACCCAT CTTCCACATT 
TCCCACATTG ACCGAGTCTA CACCCTCCGA GCAGAGAGCA TAAACGAAAG GACTGCCTGG 
GTGCAGAAAA TCAAAGCAGC TTCTGAACTC TACATAGAGA CAGAGAAGAA GAAGCGGGAG 
AAGGCGTACC TGGTCCGCTC CCAAAGAGCC ACAGGCATTG GAAGGTTGAT GGTGAACGTG 
GTTGAAGGCA TTGAGCTGAA ACCCTGTCGC TCCCATGGAA AGAGTAACCC GTACTGCGAG 
GTGACCATGG GCTCCCAGTG TCACATCACC AAGACGATCC AGGACACTCT GAATCCCAAG 
TGGAATTCCA ACTGCCAGTT CTTCATCAGA GACCTGGAGC AGGAGGTGCT GTGCATCACT 
GTGTTTGAGA GGGACCAGTT CTCGCCAGAT GATTTTTTGG GTCGGACGGA GATCCGCGTG 
GCCGACATCA AGAAGGACCA GGGCTCCAAG GGTCCCGTTA CGAAGTGTCT CCTGCTGCAC 
GAAGTTCCCA CGGGAGAGAT CGTGGTCCGC TTGGACCTGC AGTTGTTCGA TGAGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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見積依頼

Eメール

[email protected]

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

ITSN1 cDNA ORF clone, Bison bison bison

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム