RNF135 cDNA ORF clone, Bison bison bison

Gene
Clones
gRNAs

Summary

Gene Symbol	RNF135
Entrez Gene ID	105004170
Full Name	ring finger protein 135
Gene Type	protein-coding
Organism	Bison bison bison

mRNA and Protein(s)

mRNA	Protein	Name
XM_010861701.1	XP_010860003.1	E3 ubiquitin-protein ligase RNF135

Pathways from BioSystems
Gene Ontology

The following RNF135 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RNF135 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBi80799	XM_010861701.1 Latest version!	Bison bison bison ring finger protein 135 (RNF135), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RNF135 ( XM_010861701.1 ) cDNA ORF clone, Bison bison bison -> XP_010860003.1 Bison bison bison ring finger protein 135 (RNF135), mRNA.

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CloneID	OBi80799
Clone ID Related Accession (Same CDS sequence)	XM_010861701.1
Accession Version	XM_010861701.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	E3 ubiquitin-protein ligase RNF135
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011495033.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 6% of CDS bases ##RefSeq-Attributes-END##

ATGTCAGACT CTCTGATAGA AGATGAGTTT GATTTGACCA GCTGGTTTAA CTATGAGAAC 
TGGCACAGCC AATTAGATTG TGTGGACGAC CTGGGCTGCA TCATCTGTCA CGATCTGCTG 
GCCTGGCCCG CCACGCTGCC CTGCGGCCAC ACCTTCTGCC GCGACTGCCT GCTGGACCTG 
TGGGCCGCGA GGCGCAGCCG GTTCTGCCCC ACCTGCCGCG AGGGTGCCCC CGGGCCGCCG 
CAGCTGCGGA AGAACACAAT GCTGGAGGAC CTGGCCGACA AGTACAGGCT GGCGGCGCGA 
GAGCTGGAGC CCCTGAGCCC CGGCCCGGAG CCGACTACCG CACCTCGCCC AGCGTCCCGA 
CCCGGGCGCC CCACGGCGCA GCTGCGGGTG GAGGCACCGA AGAGCAGCAC AGAAGTTGAC 
CGGGAGCTGG CAAAGCTGGT GGAACGGCTT ATAAATATTG TCAGGAAACT TCAGAGTCCG 
AGACACCTCC TAGAATCTGA ACCAGATAAA GAAGTGAGCA TTCTGGCTAT GACTTTGTTG 
GATGGGGTGG ACCTTTCCAT GGCTTCTCCC AAAGTGAGTT CCAGCCCACC TGAGCAAAAA 
ATGAAAGATA TTCTGCATGA TCTAGAAGAG ATCCAAGAAA AATTACGAGA AAACTTCACG 
CAGAAAGGGG CCCTTGAAGA ACAAACACAG GTTGAACTCC CAGAAGTTCC ATCTTCTTCC 
TCATGCCCAC TGCCTGACCA GAGCCGCTTT GCACCCAAGA AGGCTTCCTC GTTTGCTCGA 
TGGGCCATCA GTCCAACCTT TGACCTTGAG AGCCTCTCCT GTAATCTGAA GGTCTCCGTG 
GATCGCCGGA CAGTGACTGT GTCTGACTTC CCAATGCTCT ATGCCTGGAG TCCTGAGAGG 
TTTGCCTCCA GCCAGGCTTT ATGTTCCCAG GCCCTCTCTT CTGGGCAGCA TTACTGGGAA 
GTGGACACTC AGCGTTGTAG CCACTGGGCA GTCGGGGTGG CTTCACGGGG GATGAAGCGT 
GACCAGATCC TGGGAAGGAC CACGGACTCC TGGTGTGTAG AGTGGAAAGG GACCAGCCAG 
CTCTCTGCGT GGTGCATGGT TAAGGAAACT CTCCTCGGCT CAGACAGACC CAGTGTGGTG 
GGCATCTGGC TGGACCTTGA GGAGGGAAAA CTTGCCTTCT ATTCCAAGGC AGATCAGGCA 
AAGCCTCTGT TTGAGTGCCC CATCTCTGTC TCTGTTCCTC TACACCCTGC CTTCTGGCTG 
TATGGCTTAT ACCCTGGAAA TTCTCTGACC ATACGCAAAA TGAATGTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010860003.1
CDS	1..1311
Translation	MSDSLIEDEFDLTSWFNYENWHSQLDCVDDLGCIICHDLLAWPATLPCGHTFCRDCLLDLWAARRSRFCPTCREGAPGPPQLRKNTMLEDLADKYRLAARELEPLSPGPEPTTAPRPASRPGRPTAQLRVEAPKSSTEVDRELAKLVERLINIVRKLQSPRHLLESEPDKEVSILAMTLLDGVDLSMASPKVSSSPPEQKMKDILHDLEEIQEKLRENFTQKGALEEQTQVELPEVPSSSSCPLPDQSRFAPKKASSFARWAISPTFDLESLSCNLKVSVDRRTVTVSDFPMLYAWSPERFASSQALCSQALSSGQHYWEVDTQRCSHWAVGVASRGMKRDQILGRTTDSWCVEWKGTSQLSAWCMVKETLLGSDRPSVVGIWLDLEEGKLAFYSKADQAKPLFECPISVSVPLHPAFWLYGLYPGNSLTIRKMNV

Target ORF information:

RefSeq Version	XM_010861701.1
Organism	Bison bison bison
Definition	Bison bison bison ring finger protein 135 (RNF135), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010861701.1

ORF Insert Sequence:

ATGTCAGACT CTCTGATAGA AGATGAGTTT GATTTGACCA GCTGGTTTAA CTATGAGAAC 
TGGCACAGCC AATTAGATTG TGTGGACGAC CTGGGCTGCA TCATCTGTCA CGATCTGCTG 
GCCTGGCCCG CCACGCTGCC CTGCGGCCAC ACCTTCTGCC GCGACTGCCT GCTGGACCTG 
TGGGCCGCGA GGCGCAGCCG GTTCTGCCCC ACCTGCCGCG AGGGTGCCCC CGGGCCGCCG 
CAGCTGCGGA AGAACACAAT GCTGGAGGAC CTGGCCGACA AGTACAGGCT GGCGGCGCGA 
GAGCTGGAGC CCCTGAGCCC CGGCCCGGAG CCGACTACCG CACCTCGCCC AGCGTCCCGA 
CCCGGGCGCC CCACGGCGCA GCTGCGGGTG GAGGCACCGA AGAGCAGCAC AGAAGTTGAC 
CGGGAGCTGG CAAAGCTGGT GGAACGGCTT ATAAATATTG TCAGGAAACT TCAGAGTCCG 
AGACACCTCC TAGAATCTGA ACCAGATAAA GAAGTGAGCA TTCTGGCTAT GACTTTGTTG 
GATGGGGTGG ACCTTTCCAT GGCTTCTCCC AAAGTGAGTT CCAGCCCACC TGAGCAAAAA 
ATGAAAGATA TTCTGCATGA TCTAGAAGAG ATCCAAGAAA AATTACGAGA AAACTTCACG 
CAGAAAGGGG CCCTTGAAGA ACAAACACAG GTTGAACTCC CAGAAGTTCC ATCTTCTTCC 
TCATGCCCAC TGCCTGACCA GAGCCGCTTT GCACCCAAGA AGGCTTCCTC GTTTGCTCGA 
TGGGCCATCA GTCCAACCTT TGACCTTGAG AGCCTCTCCT GTAATCTGAA GGTCTCCGTG 
GATCGCCGGA CAGTGACTGT GTCTGACTTC CCAATGCTCT ATGCCTGGAG TCCTGAGAGG 
TTTGCCTCCA GCCAGGCTTT ATGTTCCCAG GCCCTCTCTT CTGGGCAGCA TTACTGGGAA 
GTGGACACTC AGCGTTGTAG CCACTGGGCA GTCGGGGTGG CTTCACGGGG GATGAAGCGT 
GACCAGATCC TGGGAAGGAC CACGGACTCC TGGTGTGTAG AGTGGAAAGG GACCAGCCAG 
CTCTCTGCGT GGTGCATGGT TAAGGAAACT CTCCTCGGCT CAGACAGACC CAGTGTGGTG 
GGCATCTGGC TGGACCTTGA GGAGGGAAAA CTTGCCTTCT ATTCCAAGGC AGATCAGGCA 
AAGCCTCTGT TTGAGTGCCC CATCTCTGTC TCTGTTCCTC TACACCCTGC CTTCTGGCTG 
TATGGCTTAT ACCCTGGAAA TTCTCTGACC ATACGCAAAA TGAATGTGTA A