ITK cDNA ORF clone, Bison bison bison

Gene
Clones
gRNAs

Summary

Gene Symbol	ITK
Entrez Gene ID	104999721
Full Name	IL2 inducible T cell kinase
Gene Type	protein-coding
Organism	Bison bison bison

mRNA and Protein(s)

mRNA	Protein	Name
XM_010855281.1	XP_010853583.1	tyrosine-protein kinase ITK/TSK

Pathways from BioSystems
Gene Ontology

The following ITK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ITK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBi76061	XM_010855281.1 Latest version!	Bison bison bison IL2-inducible T-cell kinase (ITK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ITK ( XM_010855281.1 ) cDNA ORF clone, Bison bison bison -> XP_010853583.1 Bison bison bison IL2-inducible T-cell kinase (ITK), mRNA.

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CloneID	OBi76061
Clone ID Related Accession (Same CDS sequence)	XM_010855281.1
Accession Version	XM_010855281.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	tyrosine-protein kinase ITK/TSK
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011494935.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAACT TTATCCTCCT GGAAGAGCAG CTCATCAAGA AATCCCAACA AAAGAGAAGA 
ACTTCTCCTT CAAACTTTAA AGTCCGATTC TTTGTTTTAA CCAAAACCAG CCTGGCATAC 
TTTGAAGATC GTCATGGGAA AAAGCGCACT TTGAAGGGCT CCATCGAACT CTCCCGAATC 
AAATGTGTCG AGATTGTGAA AAGTGACATT ATCATCCCAT GTCACTATAA ATATCCATTT 
CAGGTTGTTC ACGACAACTA CCTCCTGTAT GTGTTTGCTC CAGACCGGGA GAGCCGGCAG 
CGCTGGGTGC TGGCCCTCAA AGAAGAAACC AGGAATAACA ACAGTTTGGT GCCCAAGTAT 
CACCCTAATT TCTGGTTGGA TGGGAGATGG CGGTGCTGTG CTCAGATGGA GAAGCTTGCA 
GTGGGCTGTG CCCAGTACGA TCCCACCAAA AATGCTTCAA AGAAGCCTCT TCCTCCTACT 
CCTGAAGACA ATAGGCGGTC ACTTCGGGAA CTTGAAGAAA CTGTGGTCAT TGCCCTGTAT 
GACTACCAAA CAAATGATCC ACAGGAACTC ATGCTACAGC GCAATGAGGA GTACTACCTG 
CTCGACAGCT CTGAGATTCA CTGGTGGAGG GTTCAGGACA GGAATGGGCA TGAAGGATAT 
GTTCCAAGCA GTTATCTGGT GGAAAAGTCT CCAAATAACC TGGAAACCTA TGAGTGGTAC 
AATAAGAACA TCAGCCGAGA CAAAGCTGAA AAACTTCTTT TGGACACAGG CAAAGAAGGA 
GCTTTTATGG TGCGAGATTC CAGGACTCCA GGGACATACA CCGTGTCCGT TTTCACCAAG 
GCCATCGTAA GTGAGAACAA CCCCTGTATA AAGCATTATC ACATCAAAGA AACAAATGAC 
AACCCTAAGC GATATTACGT GGCTGAAAAG TATGTGTTTG ATTCCATCCC TCTCCTCATC 
AATTATCACC AGCACAACGG AGGAGGCCTG GTCACTAGAC TCCGGTATCC AGTTTGCTCC 
TGGAGGCAGA AAGCCCCCGT CACCGCAGGG CTGAGATACG GGAAATGGGT GATTGATCCC 
TCAGAGCTCA CTTTTGTGCA GGAGATTGGC AGTGGGCAGT TTGGGTTGGT GCACCTTGGC 
TACTGGCTCA ACAAGGACAA GGTGGCCATC AAAACCATTC AAGAAGGGGC TATGTCAGAA 
GAGGACTTCA TTGAGGAGGC TGAAGTCATG ATGAAACTCT CTCACCCCAA ACTAGTCCAG 
CTCTATGGAG TATGCCTGGA ACAGGCCCCC ATCTGCCTGG TGTTTGAATT CATGGAACAC 
GGCTGCCTGT CAGATTACCT GCGTAGCCAG AGGGGGCTCT TTGCCGCGGA GACCCTGCTG 
GGCATGTGTC TGGACGTATG TGAGGGCATG GCCTACCTGG AAGAGGCGTG TGTCATCCAC 
AGGGACTTGG CTGCCCGGAA TTGTTTAGTT GGGGAAAACC AAGTTATCAA GGTGTCCGAC 
TTTGGGATGA CAAGGTTCGT CCTTGATGAC CAATACACCA GTTCCACAGG CACCAAATTC 
CCAGTGAAGT GGGCATCTCC GGAAGTCTTC TCCTTCAGTC GCTACAGCAG CAAGTCAGAT 
GTGTGGTCAT TTGGTGTGCT GATGTGGGAA GTCTTCAGTG AAGGCAAAAT CCCATACGAA 
AACCGAAGCA ACTCAGAGGT GGTAGAAGAC ATCACCACTG GATTTCGGTT ATACAAGCCC 
CGACTGGCCT CCCAGCATAT CTACCAGATC ATGAATCATT GCTGGAAAGA GAAACCAGAA 
GACCGGCCAC CTTTCTCCAG ACTCTTGAGT CAACTGGCTG AAATTGCAGA ATTGGGGCTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010853583.1
CDS	174..2036
Translation	MNNFILLEEQLIKKSQQKRRTSPSNFKVRFFVLTKTSLAYFEDRHGKKRTLKGSIELSRIKCVEIVKSDIIIPCHYKYPFQVVHDNYLLYVFAPDRESRQRWVLALKEETRNNNSLVPKYHPNFWLDGRWRCCAQMEKLAVGCAQYDPTKNASKKPLPPTPEDNRRSLRELEETVVIALYDYQTNDPQELMLQRNEEYYLLDSSEIHWWRVQDRNGHEGYVPSSYLVEKSPNNLETYEWYNKNISRDKAEKLLLDTGKEGAFMVRDSRTPGTYTVSVFTKAIVSENNPCIKHYHIKETNDNPKRYYVAEKYVFDSIPLLINYHQHNGGGLVTRLRYPVCSWRQKAPVTAGLRYGKWVIDPSELTFVQEIGSGQFGLVHLGYWLNKDKVAIKTIQEGAMSEEDFIEEAEVMMKLSHPKLVQLYGVCLEQAPICLVFEFMEHGCLSDYLRSQRGLFAAETLLGMCLDVCEGMAYLEEACVIHRDLAARNCLVGENQVIKVSDFGMTRFVLDDQYTSSTGTKFPVKWASPEVFSFSRYSSKSDVWSFGVLMWEVFSEGKIPYENRSNSEVVEDITTGFRLYKPRLASQHIYQIMNHCWKEKPEDRPPFSRLLSQLAEIAELGL

Target ORF information:

RefSeq Version	XM_010855281.1
Organism	Bison bison bison
Definition	Bison bison bison IL2-inducible T-cell kinase (ITK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010855281.1

ORF Insert Sequence:

ATGAACAACT TTATCCTCCT GGAAGAGCAG CTCATCAAGA AATCCCAACA AAAGAGAAGA 
ACTTCTCCTT CAAACTTTAA AGTCCGATTC TTTGTTTTAA CCAAAACCAG CCTGGCATAC 
TTTGAAGATC GTCATGGGAA AAAGCGCACT TTGAAGGGCT CCATCGAACT CTCCCGAATC 
AAATGTGTCG AGATTGTGAA AAGTGACATT ATCATCCCAT GTCACTATAA ATATCCATTT 
CAGGTTGTTC ACGACAACTA CCTCCTGTAT GTGTTTGCTC CAGACCGGGA GAGCCGGCAG 
CGCTGGGTGC TGGCCCTCAA AGAAGAAACC AGGAATAACA ACAGTTTGGT GCCCAAGTAT 
CACCCTAATT TCTGGTTGGA TGGGAGATGG CGGTGCTGTG CTCAGATGGA GAAGCTTGCA 
GTGGGCTGTG CCCAGTACGA TCCCACCAAA AATGCTTCAA AGAAGCCTCT TCCTCCTACT 
CCTGAAGACA ATAGGCGGTC ACTTCGGGAA CTTGAAGAAA CTGTGGTCAT TGCCCTGTAT 
GACTACCAAA CAAATGATCC ACAGGAACTC ATGCTACAGC GCAATGAGGA GTACTACCTG 
CTCGACAGCT CTGAGATTCA CTGGTGGAGG GTTCAGGACA GGAATGGGCA TGAAGGATAT 
GTTCCAAGCA GTTATCTGGT GGAAAAGTCT CCAAATAACC TGGAAACCTA TGAGTGGTAC 
AATAAGAACA TCAGCCGAGA CAAAGCTGAA AAACTTCTTT TGGACACAGG CAAAGAAGGA 
GCTTTTATGG TGCGAGATTC CAGGACTCCA GGGACATACA CCGTGTCCGT TTTCACCAAG 
GCCATCGTAA GTGAGAACAA CCCCTGTATA AAGCATTATC ACATCAAAGA AACAAATGAC 
AACCCTAAGC GATATTACGT GGCTGAAAAG TATGTGTTTG ATTCCATCCC TCTCCTCATC 
AATTATCACC AGCACAACGG AGGAGGCCTG GTCACTAGAC TCCGGTATCC AGTTTGCTCC 
TGGAGGCAGA AAGCCCCCGT CACCGCAGGG CTGAGATACG GGAAATGGGT GATTGATCCC 
TCAGAGCTCA CTTTTGTGCA GGAGATTGGC AGTGGGCAGT TTGGGTTGGT GCACCTTGGC 
TACTGGCTCA ACAAGGACAA GGTGGCCATC AAAACCATTC AAGAAGGGGC TATGTCAGAA 
GAGGACTTCA TTGAGGAGGC TGAAGTCATG ATGAAACTCT CTCACCCCAA ACTAGTCCAG 
CTCTATGGAG TATGCCTGGA ACAGGCCCCC ATCTGCCTGG TGTTTGAATT CATGGAACAC 
GGCTGCCTGT CAGATTACCT GCGTAGCCAG AGGGGGCTCT TTGCCGCGGA GACCCTGCTG 
GGCATGTGTC TGGACGTATG TGAGGGCATG GCCTACCTGG AAGAGGCGTG TGTCATCCAC 
AGGGACTTGG CTGCCCGGAA TTGTTTAGTT GGGGAAAACC AAGTTATCAA GGTGTCCGAC 
TTTGGGATGA CAAGGTTCGT CCTTGATGAC CAATACACCA GTTCCACAGG CACCAAATTC 
CCAGTGAAGT GGGCATCTCC GGAAGTCTTC TCCTTCAGTC GCTACAGCAG CAAGTCAGAT 
GTGTGGTCAT TTGGTGTGCT GATGTGGGAA GTCTTCAGTG AAGGCAAAAT CCCATACGAA 
AACCGAAGCA ACTCAGAGGT GGTAGAAGAC ATCACCACTG GATTTCGGTT ATACAAGCCC 
CGACTGGCCT CCCAGCATAT CTACCAGATC ATGAATCATT GCTGGAAAGA GAAACCAGAA 
GACCGGCCAC CTTTCTCCAG ACTCTTGAGT CAACTGGCTG AAATTGCAGA ATTGGGGCTT 
TAA