EED cDNA ORF clone, Bison bison bison

Gene
Clones
gRNAs

Summary

Gene Symbol	EED
Entrez Gene ID	104998185
Full Name	embryonic ectoderm development
Gene Type	protein-coding
Organism	Bison bison bison

mRNA and Protein(s)

mRNA	Protein	Name
XM_010853252.1	XP_010851554.1	polycomb protein EED

Pathways from BioSystems
Gene Ontology

The following EED gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EED cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBi74573	XM_010853252.1 Latest version!	Bison bison bison embryonic ectoderm development (EED), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EED ( XM_010853252.1 ) cDNA ORF clone, Bison bison bison -> XP_010851554.1 Bison bison bison embryonic ectoderm development (EED), mRNA.

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CloneID	OBi74573
Clone ID Related Accession (Same CDS sequence)	XM_010853252.1
Accession Version	XM_010853252.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	polycomb protein EED
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011494896.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 39% of CDS bases ##RefSeq-Attributes-END##

ATGCCCACTC TTTATGACCA GTTCATGCTG GTGCCAAATC CTATCAAGCC TCTGCCTCAG 
CAGCGGGCCG GGACAATGTG GACTCCCCCA GAAATTTTTG CCCTTCTGGG ACCAGACACT 
CATCACAAGC AAAGAAGAGG GAAACCGTAC CAAATCCTAC TTAACTGTGT GTCCACAGAA 
CCTTCTGTAG GGCTGCTGTG TACACTCGTG CAGGTTATTC ATCTAAAGAA CGGTTCCCAG 
CTGAGTTGTA GGACTATATT TCACAAACAT CCTTCTCAAC AGAATAAATA TTCATCAAGA 
AATCAGGACG CACTCAGGGG ACTACAATCG GGTGGCGCCT TGAGACCCAT CCTTTGCCAC 
GGAAACCCGG TGGAAGACTA CCTGGTTGGA AAACAAGATT TCCAAGAGGA GGGGCCTGGA 
GGAGGCGGGC CCGGACAACC AAGCGCGAGC ACACGCCCCG GCGCATGCGC ACCTGTTAGC 
GGGCACCTTC CATTAGCTAG GCAGGGGAGG CAGGCGCGAG GCTCGCTAGT TTTTGAATTT 
CTGTTCTCAA CTGCAAGTGA GGTGGCGACT AGTTCCGCGC ATAACGTGAC ACCGTTACTC 
CTCGAGTTCA AGATGAAGAA CACTCGGCAC AGACGCCCGA ACCGTGAGGC GACTTCTCGT 
CTGGTTCCCC CCGTCCCCCG GGACCCGAGT CGGCCCGTCC ATTCCACAGA CTTTCGAGTC 
CTCGCAGCGG GGTCGGGGGA TCGAAGGATT GGGCCAATTG CGGCTGAACC GTCTCTCGAA 
GGAGGAAGGG GGGCGGTCGC GGGGAAGGGG GACGTTGGAA ATAACTGCCC TCGGAAGCCG 
ACGGATCCCA CACCCGCTCC GTGCAAACTG CCGGGAGGGC GGCGGGAAGA GGCCAAGAAG 
CAGAAGCTGA GCAGCGACGA GAATAGCAAC CCGGACCTCT CTGGAGACGA GAATGATGAT 
GCTGTCAGTA TAGAAAGCGG TACCAACACA GAACGCCCTG ATACACCTAC AAATACGCCA 
AATGCACCTG GAAGGAAGAG TTGGGGGAAG GGAAAATGGA AATCAAAGAA ATGCAAATAT 
TCTTTCAAAT GTGTAAATAG TCTCAAGGAA GATCATAATC AACCATTGTT TGGAGTTCAG 
TTTAACTGGC ACAGTAAAGA AGGAGATCCA TTAGTGTTTG CAACTGTAGG AAGCAACAGA 
GTTACCTTAT ATGAATGTCA TTCACAAGGA GAAATCCGGT TGTTGCAGTC TTATGTGGAT 
GCTGATGCTG ATGAAAACTT TTACACTTGT GCGTGGACAT ATGATAGCAA TACAAGCCAT 
CCCCTGCTGG CTGTTGCTGG ATCTAGAGGC ATAATTAGGA TAATTAATCC CATAACAATG 
CAGTGCATAA AGCACTATGT TGGCCATGGA AATGCTATCA ATGAGCTGAA ATTCCACCCA 
AGAGATCCAA ATCTTCTCCT GTCAGTAAGT AAAGATCATG CTTTACGATT ATGGAATATC 
CAGACGGACA CTCTGGTGGC AATATTTGGA GGTGTAGAAG GGCACAGAGA TGAAGTTCTA 
AGTGCTGATT ATGATCTTTT GGGTGAAAAA ATAATGTCTT GTGGTATGGA TCACTCTCTT 
AAACTTTGGA GGATCAATTC AAAGAGAATG ATGAATGCGA TTAAGGAATC TTATGATTAT 
AACCCAAATA AAACTAACAG GCCATTTATT TCTCAGAAAA TTCATTTTCC TGACTTTTCT 
ACCAGAGACA TACATAGAAA TTATGTCGAT TGTGTACGAT GGTTAGGTGA TTTGATACTT 
TCCAAGAGTG GCCGTGCCAT TTTACATTCC CACCAGCAAT GTATGAAAGA TGCAGTGTCT 
TCGAATCTTC GCCAGCATTT GTCTTGTGAA AATGCCATTG TATGCTGGAA ACCTGGCAAA 
ATGGAAGATG ATATAGATAA AATTAAACCC AGTGAGTCTA ATGTGACTAT TCTTGGGCGA 
TTTGATTACA GCCAGTGTGA CATTTGGTAC ATGAGGTTTT CTATGGATTT CTGGCAAAAG 
ATGCTCGCAT TGGGCAATCA GGTTGGCAAA CTTTATGTTT GGGATTTAGA AGTAGAAGAT 
CCTCATAAAG CCAAATGCAC AACGCTGACT CATCACAAAT GCGGGGCTGC TATTCGACAA 
ACCAGTTTCA GCAGGGACAG CAGCATTCTT ATAGCTGTTT GTGATGATGC CAGCATTTGG 
CGCTGGGACC GACTGCGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010851554.1
CDS	1..2241
Translation	MPTLYDQFMLVPNPIKPLPQQRAGTMWTPPEIFALLGPDTHHKQRRGKPYQILLNCVSTEPSVGLLCTLVQVIHLKNGSQLSCRTIFHKHPSQQNKYSSRNQDALRGLQSGGALRPILCHGNPVEDYLVGKQDFQEEGPGGGGPGQPSASTRPGACAPVSGHLPLARQGRQARGSLVFEFLFSTASEVATSSAHNVTPLLLEFKMKNTRHRRPNREATSRLVPPVPRDPSRPVHSTDFRVLAAGSGDRRIGPIAAEPSLEGGRGAVAGKGDVGNNCPRKPTDPTPAPCKLPGGRREEAKKQKLSSDENSNPDLSGDENDDAVSIESGTNTERPDTPTNTPNAPGRKSWGKGKWKSKKCKYSFKCVNSLKEDHNQPLFGVQFNWHSKEGDPLVFATVGSNRVTLYECHSQGEIRLLQSYVDADADENFYTCAWTYDSNTSHPLLAVAGSRGIIRIINPITMQCIKHYVGHGNAINELKFHPRDPNLLLSVSKDHALRLWNIQTDTLVAIFGGVEGHRDEVLSADYDLLGEKIMSCGMDHSLKLWRINSKRMMNAIKESYDYNPNKTNRPFISQKIHFPDFSTRDIHRNYVDCVRWLGDLILSKSGRAILHSHQQCMKDAVSSNLRQHLSCENAIVCWKPGKMEDDIDKIKPSESNVTILGRFDYSQCDIWYMRFSMDFWQKMLALGNQVGKLYVWDLEVEDPHKAKCTTLTHHKCGAAIRQTSFSRDSSILIAVCDDASIWRWDRLR

Target ORF information:

RefSeq Version	XM_010853252.1
Organism	Bison bison bison
Definition	Bison bison bison embryonic ectoderm development (EED), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010853252.1

ORF Insert Sequence:

ATGCCCACTC TTTATGACCA GTTCATGCTG GTGCCAAATC CTATCAAGCC TCTGCCTCAG 
CAGCGGGCCG GGACAATGTG GACTCCCCCA GAAATTTTTG CCCTTCTGGG ACCAGACACT 
CATCACAAGC AAAGAAGAGG GAAACCGTAC CAAATCCTAC TTAACTGTGT GTCCACAGAA 
CCTTCTGTAG GGCTGCTGTG TACACTCGTG CAGGTTATTC ATCTAAAGAA CGGTTCCCAG 
CTGAGTTGTA GGACTATATT TCACAAACAT CCTTCTCAAC AGAATAAATA TTCATCAAGA 
AATCAGGACG CACTCAGGGG ACTACAATCG GGTGGCGCCT TGAGACCCAT CCTTTGCCAC 
GGAAACCCGG TGGAAGACTA CCTGGTTGGA AAACAAGATT TCCAAGAGGA GGGGCCTGGA 
GGAGGCGGGC CCGGACAACC AAGCGCGAGC ACACGCCCCG GCGCATGCGC ACCTGTTAGC 
GGGCACCTTC CATTAGCTAG GCAGGGGAGG CAGGCGCGAG GCTCGCTAGT TTTTGAATTT 
CTGTTCTCAA CTGCAAGTGA GGTGGCGACT AGTTCCGCGC ATAACGTGAC ACCGTTACTC 
CTCGAGTTCA AGATGAAGAA CACTCGGCAC AGACGCCCGA ACCGTGAGGC GACTTCTCGT 
CTGGTTCCCC CCGTCCCCCG GGACCCGAGT CGGCCCGTCC ATTCCACAGA CTTTCGAGTC 
CTCGCAGCGG GGTCGGGGGA TCGAAGGATT GGGCCAATTG CGGCTGAACC GTCTCTCGAA 
GGAGGAAGGG GGGCGGTCGC GGGGAAGGGG GACGTTGGAA ATAACTGCCC TCGGAAGCCG 
ACGGATCCCA CACCCGCTCC GTGCAAACTG CCGGGAGGGC GGCGGGAAGA GGCCAAGAAG 
CAGAAGCTGA GCAGCGACGA GAATAGCAAC CCGGACCTCT CTGGAGACGA GAATGATGAT 
GCTGTCAGTA TAGAAAGCGG TACCAACACA GAACGCCCTG ATACACCTAC AAATACGCCA 
AATGCACCTG GAAGGAAGAG TTGGGGGAAG GGAAAATGGA AATCAAAGAA ATGCAAATAT 
TCTTTCAAAT GTGTAAATAG TCTCAAGGAA GATCATAATC AACCATTGTT TGGAGTTCAG 
TTTAACTGGC ACAGTAAAGA AGGAGATCCA TTAGTGTTTG CAACTGTAGG AAGCAACAGA 
GTTACCTTAT ATGAATGTCA TTCACAAGGA GAAATCCGGT TGTTGCAGTC TTATGTGGAT 
GCTGATGCTG ATGAAAACTT TTACACTTGT GCGTGGACAT ATGATAGCAA TACAAGCCAT 
CCCCTGCTGG CTGTTGCTGG ATCTAGAGGC ATAATTAGGA TAATTAATCC CATAACAATG 
CAGTGCATAA AGCACTATGT TGGCCATGGA AATGCTATCA ATGAGCTGAA ATTCCACCCA 
AGAGATCCAA ATCTTCTCCT GTCAGTAAGT AAAGATCATG CTTTACGATT ATGGAATATC 
CAGACGGACA CTCTGGTGGC AATATTTGGA GGTGTAGAAG GGCACAGAGA TGAAGTTCTA 
AGTGCTGATT ATGATCTTTT GGGTGAAAAA ATAATGTCTT GTGGTATGGA TCACTCTCTT 
AAACTTTGGA GGATCAATTC AAAGAGAATG ATGAATGCGA TTAAGGAATC TTATGATTAT 
AACCCAAATA AAACTAACAG GCCATTTATT TCTCAGAAAA TTCATTTTCC TGACTTTTCT 
ACCAGAGACA TACATAGAAA TTATGTCGAT TGTGTACGAT GGTTAGGTGA TTTGATACTT 
TCCAAGAGTG GCCGTGCCAT TTTACATTCC CACCAGCAAT GTATGAAAGA TGCAGTGTCT 
TCGAATCTTC GCCAGCATTT GTCTTGTGAA AATGCCATTG TATGCTGGAA ACCTGGCAAA 
ATGGAAGATG ATATAGATAA AATTAAACCC AGTGAGTCTA ATGTGACTAT TCTTGGGCGA 
TTTGATTACA GCCAGTGTGA CATTTGGTAC ATGAGGTTTT CTATGGATTT CTGGCAAAAG 
ATGCTCGCAT TGGGCAATCA GGTTGGCAAA CTTTATGTTT GGGATTTAGA AGTAGAAGAT 
CCTCATAAAG CCAAATGCAC AACGCTGACT CATCACAAAT GCGGGGCTGC TATTCGACAA 
ACCAGTTTCA GCAGGGACAG CAGCATTCTT ATAGCTGTTT GTGATGATGC CAGCATTTGG 
CGCTGGGACC GACTGCGATA A