RFWD3 cDNA ORF clone, Bison bison bison

Gene
Clones
gRNAs

Summary

Gene Symbol	RFWD3
Entrez Gene ID	104996471
Full Name	ring finger and WD repeat domain 3
Gene Type	protein-coding
Organism	Bison bison bison

mRNA and Protein(s)

mRNA	Protein	Name
XM_010850734.1	XP_010849036.1	E3 ubiquitin-protein ligase RFWD3 isoform X2
XM_010850732.1	XP_010849034.1	E3 ubiquitin-protein ligase RFWD3 isoform X1

Pathways from BioSystems
Gene Ontology

The following RFWD3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RFWD3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBi72807	XM_010850732.1 Latest version!	Bison bison bison ring finger and WD repeat domain 3 (RFWD3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBi72808	XM_010850734.1 Latest version!	Bison bison bison ring finger and WD repeat domain 3 (RFWD3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RFWD3 ( XM_010850732.1 ) cDNA ORF clone, Bison bison bison -> XP_010849034.1 Bison bison bison ring finger and WD repeat domain 3 (RFWD3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBi72807
Clone ID Related Accession (Same CDS sequence)	XM_010850732.1
Accession Version	XM_010850732.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	E3 ubiquitin-protein ligase RFWD3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011494867.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCATG AAGCAATGGA ATATGATCTT CAGGTGCAAT TAGATCATGG TGCTGCAGAA 
CAGCCTGCTC CTGCTGAGGT GGTCAGCAGC CAAGGGGGAC CACCCTTGCT CCAGCCTCCT 
CATACTGAGG AGGTCAGCAG CCAGGAGGGA CCACCCATGT TGCATCCTGT ACTTGCTGAG 
GTGGTCAGCA TCGAAGGAGG ACCACCTCTG CTCCAACCCA TTCCTGAGGA GCTGATCAAC 
AGCCCAGAGA GAGTGCCGCT GCTTCCGCCT ATTCCACAGA TACCTATTGA TCTAGAGGAA 
GTGGAGCTCT CAGAAACAGA GACTGTGGGG AACCTCAATC CAGGAGTTTT AGAGGAGTTT 
AGACCGAGAT CTAACCACAC TCCCCAAGTG ACTTCATTGG ATTCAATGAA CAGCTTCATC 
AGTGGGCTGC AGAGACTTCA TGGCATGCTG GAATTCCTGC GACCAACTTC AGACCGCAGA 
GTGGGGCCCG TGAGAGCGAG GAGGAGTAGG AGGAGTTCTG CTTCACGGAG GGCAAGAGCA 
GGAGGGTCCC AGAGGACAAA CAGTGCCAGG TTGAGAGCAC CGTTGGATGC TTTCTTTCAA 
GCGAGCAGGA CCCAGCCTCA TTCATCAACC ACCTCTTATG ATTCAGAGAC TAGGAATCTT 
GTCTCTGATG ACTTGCAGGT ATCAGGTAGT TCTGATTCTG ACAGTGACAG CTCCACAGAG 
TTTGAGGAGG GAGTTGCCCA GGCAGAGGAG CCTGAAGCTG TTGTTTTAGA AGAGGAACTA 
GAAGACACCT CAACAGACCA AGAAGTTATA TGTGCTGGTG GAGAAGACAC TGTCCCCAAA 
CAGCAGTCTC CCCAGAAGTC TAACCCTTCT CTAACTTCTG CTTCTGTGGA TGAGGAAGAA 
GGAGACACTT GCACAATATG TTTGGAGCAG TGGACCAATG CTGGGGATCA CCGGCTCTCT 
ACATTGCGCT GTGGGCACCT CTTTGGGTAC AAATGCATTT CGAAATGGCT CAGAGGACAG 
ACCCGAAAAT GTCCCCAGTG TAACAAGAAA GCCAAGCAAA GTGATATCGT TGTCCTTTAT 
GCCCGGACCT TGAGAGCTTT GGACACTAGT GAGCAGGAGC TCATGAAGAG TTCCTTACTA 
AAGGAACAGA AGCTAAGGAA GAAGGCTGAA TTAGACTCGG CACAGTGTCG GCTGCAGCTT 
CAAGCCCTCA CTGATAAATG CTCTAGGCTT CAAAGTCATG TTCAGGACTT GCAGAAACTT 
ATCGGTCAGG ATAAGATCTC ACAACAGCCT GGGGGTTCCC AGCCACATTT CCTAAGTGGC 
CTGCCCACCA GCCAGAGTCA ACAGAAGTAC CATTTCCAGA AGACATTCAC AGTGTCTCAA 
ACAGGAAACT GTCGAGTCAT GACATACTGT GATGCTTTGA GCTGCCTGGT GGTATCACAG 
CCTTCTCCTC AGTCCGCCTT CCTTCCAGGC TTTGGTGTTA AGATGTTGAG TACTGCCAAC 
ATGAAAAGCA GCCAGTATAT TCCTATGCAT GGCAAACAAA TACGTGGACT GGCTTTCAGC 
AGTCGATCCA AAGGCTTACT TCTCTCTGCT TCCCTGGACA GCACAATTAA ACTGACCAGC 
CTGGAAACAA ATACCGTGGT CCAGACTTAC AACGCTGGGC GGCCTGTCTG GAGCTGCTGC 
TGGTGTCTTG ATGAAAACAA TTACATCTAT GCTGGACTGG TCAACGGTTC GATTCTGTTA 
TACGACCTTC GAAATACAAG CTCTCACGTC CAGGAATTAG AACCTCAGAG AGCTAGATGC 
CCGCTGGTAT CCCTGTCGTA TGTGCCCAGA GCTGCCTCTG CTGCGTTTCC ATATGGTGGC 
GTGTTGGCTG GAGCATTGGA GAATGCCTCT TTTTGGGAGC TGAAAATGGG TTTTACTCAT 
TGGCCTCATG TGCTGCCCAT GGAGCCTGGG GGCTGCGTAG ACTTTCAGGT AGAGAGCAGC 
ACCCGCCACT GTCTCGTGAC CTATAGGCCT GATAAAAATC ACAACAACTT ACGAAGTGTA 
CTCATGGAGA TGTCCTATAA ACTGGATAAT GCTGGAGAGC CCATCTGCTC CTGCCATCCT 
GTACAAACAT TCTTTGGGGG ACCCTCCTGC AAATTGCTAA CCAAGAATGC CATTTTCCAA 
AATCCAGAGA ATAATGGCAG CATCCTGGTG TGTATTGGGG ATGAAGCATC AAAATCTGCC 
CTGCTGTGGG ATGCTGGCAG TGGCTCATTG CTGCAGGATC TGCAGGCCGA TCAGCCTGTC 
TTGGACATCT GCCCATTTGA TGTGAATCAT AACAGCTACT TGGCTACCTT AACAGAAAAG 
ACCGTCCACA TCTATAGGTG GGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010849034.1
CDS	130..2496
Translation	MAHEAMEYDLQVQLDHGAAEQPAPAEVVSSQGGPPLLQPPHTEEVSSQEGPPMLHPVLAEVVSIEGGPPLLQPIPEELINSPERVPLLPPIPQIPIDLEEVELSETETVGNLNPGVLEEFRPRSNHTPQVTSLDSMNSFISGLQRLHGMLEFLRPTSDRRVGPVRARRSRRSSASRRARAGGSQRTNSARLRAPLDAFFQASRTQPHSSTTSYDSETRNLVSDDLQVSGSSDSDSDSSTEFEEGVAQAEEPEAVVLEEELEDTSTDQEVICAGGEDTVPKQQSPQKSNPSLTSASVDEEEGDTCTICLEQWTNAGDHRLSTLRCGHLFGYKCISKWLRGQTRKCPQCNKKAKQSDIVVLYARTLRALDTSEQELMKSSLLKEQKLRKKAELDSAQCRLQLQALTDKCSRLQSHVQDLQKLIGQDKISQQPGGSQPHFLSGLPTSQSQQKYHFQKTFTVSQTGNCRVMTYCDALSCLVVSQPSPQSAFLPGFGVKMLSTANMKSSQYIPMHGKQIRGLAFSSRSKGLLLSASLDSTIKLTSLETNTVVQTYNAGRPVWSCCWCLDENNYIYAGLVNGSILLYDLRNTSSHVQELEPQRARCPLVSLSYVPRAASAAFPYGGVLAGALENASFWELKMGFTHWPHVLPMEPGGCVDFQVESSTRHCLVTYRPDKNHNNLRSVLMEMSYKLDNAGEPICSCHPVQTFFGGPSCKLLTKNAIFQNPENNGSILVCIGDEASKSALLWDAGSGSLLQDLQADQPVLDICPFDVNHNSYLATLTEKTVHIYRWE

Target ORF information:

RefSeq Version	XM_010850732.1
Organism	Bison bison bison
Definition	Bison bison bison ring finger and WD repeat domain 3 (RFWD3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010850732.1

ORF Insert Sequence:

ATGGCTCATG AAGCAATGGA ATATGATCTT CAGGTGCAAT TAGATCATGG TGCTGCAGAA 
CAGCCTGCTC CTGCTGAGGT GGTCAGCAGC CAAGGGGGAC CACCCTTGCT CCAGCCTCCT 
CATACTGAGG AGGTCAGCAG CCAGGAGGGA CCACCCATGT TGCATCCTGT ACTTGCTGAG 
GTGGTCAGCA TCGAAGGAGG ACCACCTCTG CTCCAACCCA TTCCTGAGGA GCTGATCAAC 
AGCCCAGAGA GAGTGCCGCT GCTTCCGCCT ATTCCACAGA TACCTATTGA TCTAGAGGAA 
GTGGAGCTCT CAGAAACAGA GACTGTGGGG AACCTCAATC CAGGAGTTTT AGAGGAGTTT 
AGACCGAGAT CTAACCACAC TCCCCAAGTG ACTTCATTGG ATTCAATGAA CAGCTTCATC 
AGTGGGCTGC AGAGACTTCA TGGCATGCTG GAATTCCTGC GACCAACTTC AGACCGCAGA 
GTGGGGCCCG TGAGAGCGAG GAGGAGTAGG AGGAGTTCTG CTTCACGGAG GGCAAGAGCA 
GGAGGGTCCC AGAGGACAAA CAGTGCCAGG TTGAGAGCAC CGTTGGATGC TTTCTTTCAA 
GCGAGCAGGA CCCAGCCTCA TTCATCAACC ACCTCTTATG ATTCAGAGAC TAGGAATCTT 
GTCTCTGATG ACTTGCAGGT ATCAGGTAGT TCTGATTCTG ACAGTGACAG CTCCACAGAG 
TTTGAGGAGG GAGTTGCCCA GGCAGAGGAG CCTGAAGCTG TTGTTTTAGA AGAGGAACTA 
GAAGACACCT CAACAGACCA AGAAGTTATA TGTGCTGGTG GAGAAGACAC TGTCCCCAAA 
CAGCAGTCTC CCCAGAAGTC TAACCCTTCT CTAACTTCTG CTTCTGTGGA TGAGGAAGAA 
GGAGACACTT GCACAATATG TTTGGAGCAG TGGACCAATG CTGGGGATCA CCGGCTCTCT 
ACATTGCGCT GTGGGCACCT CTTTGGGTAC AAATGCATTT CGAAATGGCT CAGAGGACAG 
ACCCGAAAAT GTCCCCAGTG TAACAAGAAA GCCAAGCAAA GTGATATCGT TGTCCTTTAT 
GCCCGGACCT TGAGAGCTTT GGACACTAGT GAGCAGGAGC TCATGAAGAG TTCCTTACTA 
AAGGAACAGA AGCTAAGGAA GAAGGCTGAA TTAGACTCGG CACAGTGTCG GCTGCAGCTT 
CAAGCCCTCA CTGATAAATG CTCTAGGCTT CAAAGTCATG TTCAGGACTT GCAGAAACTT 
ATCGGTCAGG ATAAGATCTC ACAACAGCCT GGGGGTTCCC AGCCACATTT CCTAAGTGGC 
CTGCCCACCA GCCAGAGTCA ACAGAAGTAC CATTTCCAGA AGACATTCAC AGTGTCTCAA 
ACAGGAAACT GTCGAGTCAT GACATACTGT GATGCTTTGA GCTGCCTGGT GGTATCACAG 
CCTTCTCCTC AGTCCGCCTT CCTTCCAGGC TTTGGTGTTA AGATGTTGAG TACTGCCAAC 
ATGAAAAGCA GCCAGTATAT TCCTATGCAT GGCAAACAAA TACGTGGACT GGCTTTCAGC 
AGTCGATCCA AAGGCTTACT TCTCTCTGCT TCCCTGGACA GCACAATTAA ACTGACCAGC 
CTGGAAACAA ATACCGTGGT CCAGACTTAC AACGCTGGGC GGCCTGTCTG GAGCTGCTGC 
TGGTGTCTTG ATGAAAACAA TTACATCTAT GCTGGACTGG TCAACGGTTC GATTCTGTTA 
TACGACCTTC GAAATACAAG CTCTCACGTC CAGGAATTAG AACCTCAGAG AGCTAGATGC 
CCGCTGGTAT CCCTGTCGTA TGTGCCCAGA GCTGCCTCTG CTGCGTTTCC ATATGGTGGC 
GTGTTGGCTG GAGCATTGGA GAATGCCTCT TTTTGGGAGC TGAAAATGGG TTTTACTCAT 
TGGCCTCATG TGCTGCCCAT GGAGCCTGGG GGCTGCGTAG ACTTTCAGGT AGAGAGCAGC 
ACCCGCCACT GTCTCGTGAC CTATAGGCCT GATAAAAATC ACAACAACTT ACGAAGTGTA 
CTCATGGAGA TGTCCTATAA ACTGGATAAT GCTGGAGAGC CCATCTGCTC CTGCCATCCT 
GTACAAACAT TCTTTGGGGG ACCCTCCTGC AAATTGCTAA CCAAGAATGC CATTTTCCAA 
AATCCAGAGA ATAATGGCAG CATCCTGGTG TGTATTGGGG ATGAAGCATC AAAATCTGCC 
CTGCTGTGGG ATGCTGGCAG TGGCTCATTG CTGCAGGATC TGCAGGCCGA TCAGCCTGTC 
TTGGACATCT GCCCATTTGA TGTGAATCAT AACAGCTACT TGGCTACCTT AACAGAAAAG 
ACCGTCCACA TCTATAGGTG GGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD3 ( XM_010850734.1 ) cDNA ORF clone, Bison bison bison -> XP_010849036.1 Bison bison bison ring finger and WD repeat domain 3 (RFWD3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBi72808
Clone ID Related Accession (Same CDS sequence)	XM_010850734.1
Accession Version	XM_010850734.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	E3 ubiquitin-protein ligase RFWD3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011494867.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCATG AAGCAATGGA ATATGATCTT CAGGTGCAAT TAGATCATGG TGCTGCAGAA 
CAGCCTGCTC CTGCTGAGGT GGTCAGCAGC CAAGGGGGAC CACCCTTGCT CCAGCCTCCT 
CATACTGAGG AGGTCAGCAG CCAGGAGGGA CCACCCATGT TGCATCCTGT ACTTGCTGAG 
GTGGTCAGCA TCGAAGGAGG ACCACCTCTG CTCCAACCCA TTCCTGAGGA GCTGATCAAC 
AGCCCAGAGA GAGTGCCGCT GCTTCCGCCT ATTCCACAGA TACCTATTGA TCTAGAGGAA 
GTGGAGCTCT CAGAAACAGA GACTGTGGGG AACCTCAATC CAGGAGTTTT AGAGGAGTTT 
AGACCGAGAT CTAACCACAC TCCCCAAGTG ACTTCATTGG ATTCAATGAA CAGCTTCATC 
AGTGGGCTGC AGAGACTTCA TGGCATGCTG GAATTCCTGC GACCAACTTC AGACCGCAGA 
GTGGGGCCCG TGAGAGCGAG GAGGAGTAGG AGGAGTTCTG CTTCACGGAG GGCAAGAGCA 
GGAGGGTCCC AGAGGACAAA CAGTGCCAGG TTGAGAGCAC CGTTGGATGC TTTCTTTCAA 
GCGAGCAGGA CCCAGCCTCA TTCATCAACC ACCTCTTATG ATTCAGAGAC TAGGAATCTT 
GTCTCTGATG ACTTGCAGGT ATCAGGTAGT TCTGATTCTG ACAGTGACAG CTCCACAGAG 
TTTGAGGAGG GAGTTGCCCA GGCAGAGGAG CCTGAAGCTG TTGTTTTAGA AGAGGAACTA 
GAAGACACCT CAACAGACCA AGAAGTTATA TGTGCTGGTG GAGAAGACAC TGTCCCCAAA 
CAGTCTCCCC AGAAGTCTAA CCCTTCTCTA ACTTCTGCTT CTGTGGATGA GGAAGAAGGA 
GACACTTGCA CAATATGTTT GGAGCAGTGG ACCAATGCTG GGGATCACCG GCTCTCTACA 
TTGCGCTGTG GGCACCTCTT TGGGTACAAA TGCATTTCGA AATGGCTCAG AGGACAGACC 
CGAAAATGTC CCCAGTGTAA CAAGAAAGCC AAGCAAAGTG ATATCGTTGT CCTTTATGCC 
CGGACCTTGA GAGCTTTGGA CACTAGTGAG CAGGAGCTCA TGAAGAGTTC CTTACTAAAG 
GAACAGAAGC TAAGGAAGAA GGCTGAATTA GACTCGGCAC AGTGTCGGCT GCAGCTTCAA 
GCCCTCACTG ATAAATGCTC TAGGCTTCAA AGTCATGTTC AGGACTTGCA GAAACTTATC 
GGTCAGGATA AGATCTCACA ACAGCCTGGG GGTTCCCAGC CACATTTCCT AAGTGGCCTG 
CCCACCAGCC AGAGTCAACA GAAGTACCAT TTCCAGAAGA CATTCACAGT GTCTCAAACA 
GGAAACTGTC GAGTCATGAC ATACTGTGAT GCTTTGAGCT GCCTGGTGGT ATCACAGCCT 
TCTCCTCAGT CCGCCTTCCT TCCAGGCTTT GGTGTTAAGA TGTTGAGTAC TGCCAACATG 
AAAAGCAGCC AGTATATTCC TATGCATGGC AAACAAATAC GTGGACTGGC TTTCAGCAGT 
CGATCCAAAG GCTTACTTCT CTCTGCTTCC CTGGACAGCA CAATTAAACT GACCAGCCTG 
GAAACAAATA CCGTGGTCCA GACTTACAAC GCTGGGCGGC CTGTCTGGAG CTGCTGCTGG 
TGTCTTGATG AAAACAATTA CATCTATGCT GGACTGGTCA ACGGTTCGAT TCTGTTATAC 
GACCTTCGAA ATACAAGCTC TCACGTCCAG GAATTAGAAC CTCAGAGAGC TAGATGCCCG 
CTGGTATCCC TGTCGTATGT GCCCAGAGCT GCCTCTGCTG CGTTTCCATA TGGTGGCGTG 
TTGGCTGGAG CATTGGAGAA TGCCTCTTTT TGGGAGCTGA AAATGGGTTT TACTCATTGG 
CCTCATGTGC TGCCCATGGA GCCTGGGGGC TGCGTAGACT TTCAGGTAGA GAGCAGCACC 
CGCCACTGTC TCGTGACCTA TAGGCCTGAT AAAAATCACA ACAACTTACG AAGTGTACTC 
ATGGAGATGT CCTATAAACT GGATAATGCT GGAGAGCCCA TCTGCTCCTG CCATCCTGTA 
CAAACATTCT TTGGGGGACC CTCCTGCAAA TTGCTAACCA AGAATGCCAT TTTCCAAAAT 
CCAGAGAATA ATGGCAGCAT CCTGGTGTGT ATTGGGGATG AAGCATCAAA ATCTGCCCTG 
CTGTGGGATG CTGGCAGTGG CTCATTGCTG CAGGATCTGC AGGCCGATCA GCCTGTCTTG 
GACATCTGCC CATTTGATGT GAATCATAAC AGCTACTTGG CTACCTTAAC AGAAAAGACC 
GTCCACATCT ATAGGTGGGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010849036.1
CDS	130..2493
Translation	MAHEAMEYDLQVQLDHGAAEQPAPAEVVSSQGGPPLLQPPHTEEVSSQEGPPMLHPVLAEVVSIEGGPPLLQPIPEELINSPERVPLLPPIPQIPIDLEEVELSETETVGNLNPGVLEEFRPRSNHTPQVTSLDSMNSFISGLQRLHGMLEFLRPTSDRRVGPVRARRSRRSSASRRARAGGSQRTNSARLRAPLDAFFQASRTQPHSSTTSYDSETRNLVSDDLQVSGSSDSDSDSSTEFEEGVAQAEEPEAVVLEEELEDTSTDQEVICAGGEDTVPKQSPQKSNPSLTSASVDEEEGDTCTICLEQWTNAGDHRLSTLRCGHLFGYKCISKWLRGQTRKCPQCNKKAKQSDIVVLYARTLRALDTSEQELMKSSLLKEQKLRKKAELDSAQCRLQLQALTDKCSRLQSHVQDLQKLIGQDKISQQPGGSQPHFLSGLPTSQSQQKYHFQKTFTVSQTGNCRVMTYCDALSCLVVSQPSPQSAFLPGFGVKMLSTANMKSSQYIPMHGKQIRGLAFSSRSKGLLLSASLDSTIKLTSLETNTVVQTYNAGRPVWSCCWCLDENNYIYAGLVNGSILLYDLRNTSSHVQELEPQRARCPLVSLSYVPRAASAAFPYGGVLAGALENASFWELKMGFTHWPHVLPMEPGGCVDFQVESSTRHCLVTYRPDKNHNNLRSVLMEMSYKLDNAGEPICSCHPVQTFFGGPSCKLLTKNAIFQNPENNGSILVCIGDEASKSALLWDAGSGSLLQDLQADQPVLDICPFDVNHNSYLATLTEKTVHIYRWE

Target ORF information:

RefSeq Version	XM_010850734.1
Organism	Bison bison bison
Definition	Bison bison bison ring finger and WD repeat domain 3 (RFWD3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010850734.1

ORF Insert Sequence:

ATGGCTCATG AAGCAATGGA ATATGATCTT CAGGTGCAAT TAGATCATGG TGCTGCAGAA 
CAGCCTGCTC CTGCTGAGGT GGTCAGCAGC CAAGGGGGAC CACCCTTGCT CCAGCCTCCT 
CATACTGAGG AGGTCAGCAG CCAGGAGGGA CCACCCATGT TGCATCCTGT ACTTGCTGAG 
GTGGTCAGCA TCGAAGGAGG ACCACCTCTG CTCCAACCCA TTCCTGAGGA GCTGATCAAC 
AGCCCAGAGA GAGTGCCGCT GCTTCCGCCT ATTCCACAGA TACCTATTGA TCTAGAGGAA 
GTGGAGCTCT CAGAAACAGA GACTGTGGGG AACCTCAATC CAGGAGTTTT AGAGGAGTTT 
AGACCGAGAT CTAACCACAC TCCCCAAGTG ACTTCATTGG ATTCAATGAA CAGCTTCATC 
AGTGGGCTGC AGAGACTTCA TGGCATGCTG GAATTCCTGC GACCAACTTC AGACCGCAGA 
GTGGGGCCCG TGAGAGCGAG GAGGAGTAGG AGGAGTTCTG CTTCACGGAG GGCAAGAGCA 
GGAGGGTCCC AGAGGACAAA CAGTGCCAGG TTGAGAGCAC CGTTGGATGC TTTCTTTCAA 
GCGAGCAGGA CCCAGCCTCA TTCATCAACC ACCTCTTATG ATTCAGAGAC TAGGAATCTT 
GTCTCTGATG ACTTGCAGGT ATCAGGTAGT TCTGATTCTG ACAGTGACAG CTCCACAGAG 
TTTGAGGAGG GAGTTGCCCA GGCAGAGGAG CCTGAAGCTG TTGTTTTAGA AGAGGAACTA 
GAAGACACCT CAACAGACCA AGAAGTTATA TGTGCTGGTG GAGAAGACAC TGTCCCCAAA 
CAGTCTCCCC AGAAGTCTAA CCCTTCTCTA ACTTCTGCTT CTGTGGATGA GGAAGAAGGA 
GACACTTGCA CAATATGTTT GGAGCAGTGG ACCAATGCTG GGGATCACCG GCTCTCTACA 
TTGCGCTGTG GGCACCTCTT TGGGTACAAA TGCATTTCGA AATGGCTCAG AGGACAGACC 
CGAAAATGTC CCCAGTGTAA CAAGAAAGCC AAGCAAAGTG ATATCGTTGT CCTTTATGCC 
CGGACCTTGA GAGCTTTGGA CACTAGTGAG CAGGAGCTCA TGAAGAGTTC CTTACTAAAG 
GAACAGAAGC TAAGGAAGAA GGCTGAATTA GACTCGGCAC AGTGTCGGCT GCAGCTTCAA 
GCCCTCACTG ATAAATGCTC TAGGCTTCAA AGTCATGTTC AGGACTTGCA GAAACTTATC 
GGTCAGGATA AGATCTCACA ACAGCCTGGG GGTTCCCAGC CACATTTCCT AAGTGGCCTG 
CCCACCAGCC AGAGTCAACA GAAGTACCAT TTCCAGAAGA CATTCACAGT GTCTCAAACA 
GGAAACTGTC GAGTCATGAC ATACTGTGAT GCTTTGAGCT GCCTGGTGGT ATCACAGCCT 
TCTCCTCAGT CCGCCTTCCT TCCAGGCTTT GGTGTTAAGA TGTTGAGTAC TGCCAACATG 
AAAAGCAGCC AGTATATTCC TATGCATGGC AAACAAATAC GTGGACTGGC TTTCAGCAGT 
CGATCCAAAG GCTTACTTCT CTCTGCTTCC CTGGACAGCA CAATTAAACT GACCAGCCTG 
GAAACAAATA CCGTGGTCCA GACTTACAAC GCTGGGCGGC CTGTCTGGAG CTGCTGCTGG 
TGTCTTGATG AAAACAATTA CATCTATGCT GGACTGGTCA ACGGTTCGAT TCTGTTATAC 
GACCTTCGAA ATACAAGCTC TCACGTCCAG GAATTAGAAC CTCAGAGAGC TAGATGCCCG 
CTGGTATCCC TGTCGTATGT GCCCAGAGCT GCCTCTGCTG CGTTTCCATA TGGTGGCGTG 
TTGGCTGGAG CATTGGAGAA TGCCTCTTTT TGGGAGCTGA AAATGGGTTT TACTCATTGG 
CCTCATGTGC TGCCCATGGA GCCTGGGGGC TGCGTAGACT TTCAGGTAGA GAGCAGCACC 
CGCCACTGTC TCGTGACCTA TAGGCCTGAT AAAAATCACA ACAACTTACG AAGTGTACTC 
ATGGAGATGT CCTATAAACT GGATAATGCT GGAGAGCCCA TCTGCTCCTG CCATCCTGTA 
CAAACATTCT TTGGGGGACC CTCCTGCAAA TTGCTAACCA AGAATGCCAT TTTCCAAAAT 
CCAGAGAATA ATGGCAGCAT CCTGGTGTGT ATTGGGGATG AAGCATCAAA ATCTGCCCTG 
CTGTGGGATG CTGGCAGTGG CTCATTGCTG CAGGATCTGC AGGCCGATCA GCCTGTCTTG 
GACATCTGCC CATTTGATGT GAATCATAAC AGCTACTTGG CTACCTTAAC AGAAAAGACC 
GTCCACATCT ATAGGTGGGA GTAA