IDE cDNA ORF clone, Bison bison bison

Gene
Clones
gRNAs

Summary

Gene Symbol	IDE
Entrez Gene ID	104990205
Full Name	insulin degrading enzyme
Gene Type	protein-coding
Organism	Bison bison bison

mRNA and Protein(s)

mRNA	Protein	Name
XM_010842200.1	XP_010840502.1	LOW QUALITY PROTEIN: insulin-degrading enzyme

Pathways from BioSystems
Gene Ontology

The following IDE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IDE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBi66415	XM_010842200.1 Latest version!	Bison bison bison insulin-degrading enzyme (IDE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IDE ( XM_010842200.1 ) cDNA ORF clone, Bison bison bison -> XP_010840502.1 Bison bison bison insulin-degrading enzyme (IDE), mRNA.

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CloneID	OBi66415
Clone ID Related Accession (Same CDS sequence)	XM_010842200.1
Accession Version	XM_010842200.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	LOW QUALITY PROTEIN: insulin-degrading enzyme
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011494744.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGCGGTACC GGCTAGCGTG GCTGCTGCAC CCCGCGCTGC CCAGCACCTT CCGCTCGGTC 
CTCGGAGCCC GCCTGCCGCC CTCGGAGCGC CTCTGTGGTT TCCAAAAAAA GACTTACAGC 
AAAATGAATA ATCCAGCAAT CAAGAGAATA GGACATCATA TCATCAAGTC TCATGAAGAC 
AAGCGAGAAT ACCGCGGACT TGAGCTGGCC AATGGGATCA AAGTACTTCT TGTCAGTGAT 
CCAACAACAG ATAAATCATC AGCAGCACTT GATGTACACA TAGGTTCATT GTCAGATCCT 
CCAAATATTG CTGGCTTAAG TCATTTTTGT GAACATATGT TATTTTTGGG AACAAAGAAA 
TACCCTAAAG AAAATGAATA CAGCCAGTTT CTCAGTGAGC ATGCAGGAAG TTCAAACGCC 
TTCACTAGTG GAGAGCATAC CAATTACTAT TTTGATGTTT CTCATGAACA CCTAGAAGGT 
GCGCTAGACA GATTTGCACA GTTTTTCCTG TGCCCCTTGT TTGATGAGAG CTGCAAAGAC 
AGAGAGGTGA ATGCAGTTGA TTCAGAACAT GAGAAGAATG TGATGAACGA TGCCTGGAGA 
CTCTTTCAGC TGGAAAAAGC TACAGGGAAT CCCAAACATC CCTTCAGTAA ATTTGGAACA 
GGTAACAAAT ATACTCTAGA GACTAGACCG AACCAAGAAG GCATTGATGT AAGACAAGAG 
CTCCTGAAAT TCCATTCTAC TTATTATTCA TCGAACTTAA TGGCTATTTG TGTTTTAGGT 
CGAGAATCTT TAGATGATTT GACTAATCTG GTGGTAAAGT TATTTTCTGA AGTAGAGAAC 
AAAAATGTCC CGTTGCCAGA ATTTCCTGAA CATCCTTTCC AAGAAGAACA TCTTAAACAA 
CTTTACAAAA TAGTACCCAT TAAGGATATT AGGAATCTTT ATGTGACATT TCCCATACCT 
GACCTTCAGA AATACTACAA ATCAAATCCC GGTCATTATC TCGGTCATCT CATTGGGCAT 
GAAGGTCCTG GAAGTCTGTT ATCAGAACTT AAATCAAAGG GCTGGGTAAA TACTCTTGTT 
GGTGGGCAAA AGGAAGGAGC CCGAGGTTTT ATGTTTTTTA TCATTAATGT GGACTTGACT 
GAGGAAGGAT TATTACATGT TGAGGATATA ATTTTGCACA TGTTTCAATA CATTCAGAAG 
TTACGAGCAG AAGGACCTCA AGAATGGGTT TTCCAAGAGT GCAAGGACTT GAATGCTGTT 
GCTTTTAGGT TTAAAGATAA AGAGAGACCA CGGGGTTATA CCTCTAAGAT TGCAGGAATA 
TTACATTATT ATCCCCTAGA AGAAGTGCTC ACAGCTGAAT ATTTACTGGA AGAATTTAGA 
CCAGATTTAA TAGAGATGGT TCTCGATAAA CTCAGACCAG AAAATGTCCG GGTTGCAATA 
GTTTCCAAAT CTTTTGAAGG AAAAACTGAT CGCACAGAAG AGTGGTACGG AACCCAGTAC 
AAACAAGAAG CTATACCAGA CGAAGTCATT AAGAAATGGC AAAATGCTGA TCTGAATGGG 
AAATTTAAAC TTCCAATGAA GAATGAATTT ATTCCTACAA ATTTTGAAAT TTTATCATTA 
GAAAAAGAAG CAACGCCATA CCCTTCTCTT ATTAAGGATA CAGCTATGAG CAAACTCTGG 
TTCAAACAAG ATGATAAGTT TTTCTTGCCC AAGGCCTGTC TCAACTTTGA ATTTTTCAGC 
CCATTTGCTT ATGTGGACCC CTTGCACTGT AACATGGCCT ATTTGTACCT TGAGCTCCTC 
AAAGACTCAC TCAACGAGTA TGCATATGCA GCAGAGCTAG CAGGCTTGAG CTATGACCTC 
CAAAATACCA TCTATGGGAT GTATCTCTCG GTGAAAGGTT ACAATGACAA GCAGCCAATT 
TTGCTAAAGA AGATCATTGA GAAAATGGCT ACCTTTGAGA TTGATGAAAA AAGATTTGAA 
ATTATCAAAG AGGCATATAT GCGATCTCTT AACAATTTCC GAGCTGAACA GCCTCATCAA 
CACGCCATGT ACTACCTCCG CTTGCTGATG ACTGAAGTGG CCTGGACTAA AGATGAATTA 
AAAGAAGCCT TGGATGATGT CACCCTTCCT CGCCTTAAGG CCTTCATACC TCAACTCCTG 
TCACGGCTGC ATATTGAAGC CCTTCTCCAT GGAAATATAA CAAAGCAGGC TGCGTTAGGA 
ATCATGCAGA TGGTTGAAGA CACCCTTATT GAACATGCTC ATACGAAACC ACTTCTTCCA 
AGTCAGCTCG TTCGGTACAG AGAAGTTCAG CTCCCGGACA GGGGATGGTT TGTGTATCAG 
CAGAGGAATG AAGTCCACAA TAACTGTGGC ATTGAGATAT ACTACCAAAC CGACATGCAG 
AGCACGTCAG AGAACATGTT TCTGGAGCTC TTCTGTCAGA TCATCTCCGA GCCTTGCTTC 
AACACCCTGC GCACCAAGGA GCAACTGGGC TATATCGTCT TCAGCGGGCC ACGCCGAGCC 
AATGGCATTC AGGGCTTACG GTTTATCATC CAGTCAGAAA AGCCACCTCA CTACCTAGAA 
AGCAGAGTGG AAGCTTTCTT AATTACCATG GAAAAGTCCA TAGAGGACAT GACAGAAGAG 
GCCTTCCAAA AACACATCCA AGCATTAGCA ATCCGTCGAC TAGACAAACC AAAGAAGCTA 
TCTGCAGAGT GTGCTAAATA TTGGGGGGAG ATCATCTCTC AGCAGTACAA TTTTGACAGA 
GATAATATAG AAGTTGCCTA TTTGAAGACA CTTACCAAGG AAGATATTAT CAAATTCTAT 
AAGGAAATGT TGGCGGTAGA TGCCCCGAGG AGACACAAGG TATCCGTCCA TGTTCTTGCC 
AGAGAAATGG ATTCTTGTCC TGTGGTTGGA GAGTTCCCAT GTCAAAATGA TATAAATTTG 
TCACAAGCAC CAGCCTTGCC ACAACCTGAA GTGATTCAGA ACATGACTGA GTTCAAGCGT 
GGTTTGCCAC TGTTCCCCCT TGTGAAACCG CATATTAACT TCATGGCTGC AAAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010840502.1
CDS	91..3150
Translation	MRYRLAWLLHPALPSTFRSVLGARLPPSERLCGFQKKTYSKMNNPAIKRIGHHIIKSHEDKREYRGLELANGIKVLLVSDPTTDKSSAALDVHIGSLSDPPNIAGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVRQELLKFHSTYYSSNLMAICVLGRESLDDLTNLVVKLFSEVENKNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFRPDLIEMVLDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAIPDEVIKKWQNADLNGKFKLPMKNEFIPTNFEILSLEKEATPYPSLIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILLKKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQQRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKSIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNIEVAYLKTLTKEDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQPEVIQNMTEFKRGLPLFPLVKPHINFMAAKL

Target ORF information:

RefSeq Version	XM_010842200.1
Organism	Bison bison bison
Definition	Bison bison bison insulin-degrading enzyme (IDE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010842200.1

ORF Insert Sequence:

ATGCGGTACC GGCTAGCGTG GCTGCTGCAC CCCGCGCTGC CCAGCACCTT CCGCTCGGTC 
CTCGGAGCCC GCCTGCCGCC CTCGGAGCGC CTCTGTGGTT TCCAAAAAAA GACTTACAGC 
AAAATGAATA ATCCAGCAAT CAAGAGAATA GGACATCATA TCATCAAGTC TCATGAAGAC 
AAGCGAGAAT ACCGCGGACT TGAGCTGGCC AATGGGATCA AAGTACTTCT TGTCAGTGAT 
CCAACAACAG ATAAATCATC AGCAGCACTT GATGTACACA TAGGTTCATT GTCAGATCCT 
CCAAATATTG CTGGCTTAAG TCATTTTTGT GAACATATGT TATTTTTGGG AACAAAGAAA 
TACCCTAAAG AAAATGAATA CAGCCAGTTT CTCAGTGAGC ATGCAGGAAG TTCAAACGCC 
TTCACTAGTG GAGAGCATAC CAATTACTAT TTTGATGTTT CTCATGAACA CCTAGAAGGT 
GCGCTAGACA GATTTGCACA GTTTTTCCTG TGCCCCTTGT TTGATGAGAG CTGCAAAGAC 
AGAGAGGTGA ATGCAGTTGA TTCAGAACAT GAGAAGAATG TGATGAACGA TGCCTGGAGA 
CTCTTTCAGC TGGAAAAAGC TACAGGGAAT CCCAAACATC CCTTCAGTAA ATTTGGAACA 
GGTAACAAAT ATACTCTAGA GACTAGACCG AACCAAGAAG GCATTGATGT AAGACAAGAG 
CTCCTGAAAT TCCATTCTAC TTATTATTCA TCGAACTTAA TGGCTATTTG TGTTTTAGGT 
CGAGAATCTT TAGATGATTT GACTAATCTG GTGGTAAAGT TATTTTCTGA AGTAGAGAAC 
AAAAATGTCC CGTTGCCAGA ATTTCCTGAA CATCCTTTCC AAGAAGAACA TCTTAAACAA 
CTTTACAAAA TAGTACCCAT TAAGGATATT AGGAATCTTT ATGTGACATT TCCCATACCT 
GACCTTCAGA AATACTACAA ATCAAATCCC GGTCATTATC TCGGTCATCT CATTGGGCAT 
GAAGGTCCTG GAAGTCTGTT ATCAGAACTT AAATCAAAGG GCTGGGTAAA TACTCTTGTT 
GGTGGGCAAA AGGAAGGAGC CCGAGGTTTT ATGTTTTTTA TCATTAATGT GGACTTGACT 
GAGGAAGGAT TATTACATGT TGAGGATATA ATTTTGCACA TGTTTCAATA CATTCAGAAG 
TTACGAGCAG AAGGACCTCA AGAATGGGTT TTCCAAGAGT GCAAGGACTT GAATGCTGTT 
GCTTTTAGGT TTAAAGATAA AGAGAGACCA CGGGGTTATA CCTCTAAGAT TGCAGGAATA 
TTACATTATT ATCCCCTAGA AGAAGTGCTC ACAGCTGAAT ATTTACTGGA AGAATTTAGA 
CCAGATTTAA TAGAGATGGT TCTCGATAAA CTCAGACCAG AAAATGTCCG GGTTGCAATA 
GTTTCCAAAT CTTTTGAAGG AAAAACTGAT CGCACAGAAG AGTGGTACGG AACCCAGTAC 
AAACAAGAAG CTATACCAGA CGAAGTCATT AAGAAATGGC AAAATGCTGA TCTGAATGGG 
AAATTTAAAC TTCCAATGAA GAATGAATTT ATTCCTACAA ATTTTGAAAT TTTATCATTA 
GAAAAAGAAG CAACGCCATA CCCTTCTCTT ATTAAGGATA CAGCTATGAG CAAACTCTGG 
TTCAAACAAG ATGATAAGTT TTTCTTGCCC AAGGCCTGTC TCAACTTTGA ATTTTTCAGC 
CCATTTGCTT ATGTGGACCC CTTGCACTGT AACATGGCCT ATTTGTACCT TGAGCTCCTC 
AAAGACTCAC TCAACGAGTA TGCATATGCA GCAGAGCTAG CAGGCTTGAG CTATGACCTC 
CAAAATACCA TCTATGGGAT GTATCTCTCG GTGAAAGGTT ACAATGACAA GCAGCCAATT 
TTGCTAAAGA AGATCATTGA GAAAATGGCT ACCTTTGAGA TTGATGAAAA AAGATTTGAA 
ATTATCAAAG AGGCATATAT GCGATCTCTT AACAATTTCC GAGCTGAACA GCCTCATCAA 
CACGCCATGT ACTACCTCCG CTTGCTGATG ACTGAAGTGG CCTGGACTAA AGATGAATTA 
AAAGAAGCCT TGGATGATGT CACCCTTCCT CGCCTTAAGG CCTTCATACC TCAACTCCTG 
TCACGGCTGC ATATTGAAGC CCTTCTCCAT GGAAATATAA CAAAGCAGGC TGCGTTAGGA 
ATCATGCAGA TGGTTGAAGA CACCCTTATT GAACATGCTC ATACGAAACC ACTTCTTCCA 
AGTCAGCTCG TTCGGTACAG AGAAGTTCAG CTCCCGGACA GGGGATGGTT TGTGTATCAG 
CAGAGGAATG AAGTCCACAA TAACTGTGGC ATTGAGATAT ACTACCAAAC CGACATGCAG 
AGCACGTCAG AGAACATGTT TCTGGAGCTC TTCTGTCAGA TCATCTCCGA GCCTTGCTTC 
AACACCCTGC GCACCAAGGA GCAACTGGGC TATATCGTCT TCAGCGGGCC ACGCCGAGCC 
AATGGCATTC AGGGCTTACG GTTTATCATC CAGTCAGAAA AGCCACCTCA CTACCTAGAA 
AGCAGAGTGG AAGCTTTCTT AATTACCATG GAAAAGTCCA TAGAGGACAT GACAGAAGAG 
GCCTTCCAAA AACACATCCA AGCATTAGCA ATCCGTCGAC TAGACAAACC AAAGAAGCTA 
TCTGCAGAGT GTGCTAAATA TTGGGGGGAG ATCATCTCTC AGCAGTACAA TTTTGACAGA 
GATAATATAG AAGTTGCCTA TTTGAAGACA CTTACCAAGG AAGATATTAT CAAATTCTAT 
AAGGAAATGT TGGCGGTAGA TGCCCCGAGG AGACACAAGG TATCCGTCCA TGTTCTTGCC 
AGAGAAATGG ATTCTTGTCC TGTGGTTGGA GAGTTCCCAT GTCAAAATGA TATAAATTTG 
TCACAAGCAC CAGCCTTGCC ACAACCTGAA GTGATTCAGA ACATGACTGA GTTCAAGCGT 
GGTTTGCCAC TGTTCCCCCT TGTGAAACCG CATATTAACT TCATGGCTGC AAAACTCTGA