CHKB cDNA ORF clone, Bison bison bison

Gene
Clones
gRNAs

Summary

Gene Symbol	CHKB
Entrez Gene ID	104989760
Full Name	choline kinase beta
Gene Type	protein-coding
Organism	Bison bison bison

mRNA and Protein(s)

mRNA	Protein	Name
XM_010841552.1	XP_010839854.1	choline/ethanolamine kinase

Pathways from BioSystems
Gene Ontology

The following CHKB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHKB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBi57752	XM_010841552.1 Latest version!	Bison bison bison choline kinase beta (CHKB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHKB ( XM_010841552.1 ) cDNA ORF clone, Bison bison bison -> XP_010839854.1 Bison bison bison choline kinase beta (CHKB), mRNA.

Price & Availability↑

CloneID	OBi57752
Clone ID Related Accession (Same CDS sequence)	XM_010841552.1
Accession Version	XM_010841552.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	choline/ethanolamine kinase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011477639.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases ##RefSeq-Attributes-END##

ATGGTTGACA CTCAGCTCAA GAGCGTTGGC GAGGAGCCCC GGGAGGTGCT GCTGCGGCTG 
TACGGGGCCA TCCTGCAGGG CGTGGACTCC TTGGTCCTTG AAAGTGTGAT GTTCGCCATA 
CTTGCAGAGC GGTCGCTGGG GCCCCAGCTC TACGGAGTCT TTCCAGAGGG CCGGCTGGAA 
CAGTACATCC CAAGCCGGCC ACTGAAGACA CATGAGCTTC GAGATCCAGT GTTGTCTGCA 
GCCATTGCCA CGAAGATGGC CAAGTTCCAC GGCATGGAGA TGCCTTTCAC TAAGGAGCCC 
CACTGGCTAT TCGGGACCAT GGAGCGGTAC TTAAAGCAGA TCCAGGACCT GCCCCCTACC 
AGCCTTCCCC AGATGAACCT GCTGGAGATG TACAGCTTGA AGGATGAGAT GGGCAACCTC 
AGGAAGCTGC TAGACACTAC CCCATCACCA GTGGTCTTTT GCCACAATGA TATCCAAGAA 
GGGAACATCT TACTGCTCTC AGAGCCTAAA AACACCGACA GCCTCATGCT GGTGGACTTC 
GAGTACAGCA GTTATAACTA CAGGGGCTTT GACATCGGGA ACCATTTTTG TGAGTGGGTT 
TACGATTATA CTCACGAGGA GTGGCCTTTC TACAAAGCGC AGCCTGCAAA CTACCCCACT 
GAGGGACAGC AGCTCCATTT TATTCGCCAC TACCTGGCAG AGGTAAAGAA AGATGAGACC 
ATCTCCCAAG AGGAGCAGAG GAAACTGGAA GCAGATTTGC TGGTGGAAGC TAATCGGTAT 
GCCCTGGCAT CTCATTTCTT TTGGGGTCTC TGGTCCATCC TTCAGGCATC CATGTCCACT 
ATAGAATTTG GTTACTTGGA GTATGCGCAG TCTCGGTTCC AGTTCTACTT CCAGCAGAAG 
GGCCAGATGA CCAGCTTCCA CCCCTCGTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010839854.1
CDS	1..933
Translation	MVDTQLKSVGEEPREVLLRLYGAILQGVDSLVLESVMFAILAERSLGPQLYGVFPEGRLEQYIPSRPLKTHELRDPVLSAAIATKMAKFHGMEMPFTKEPHWLFGTMERYLKQIQDLPPTSLPQMNLLEMYSLKDEMGNLRKLLDTTPSPVVFCHNDIQEGNILLLSEPKNTDSLMLVDFEYSSYNYRGFDIGNHFCEWVYDYTHEEWPFYKAQPANYPTEGQQLHFIRHYLAEVKKDETISQEEQRKLEADLLVEANRYALASHFFWGLWSILQASMSTIEFGYLEYAQSRFQFYFQQKGQMTSFHPSS

Target ORF information:

RefSeq Version	XM_010841552.1
Organism	Bison bison bison
Definition	Bison bison bison choline kinase beta (CHKB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010841552.1

ORF Insert Sequence:

ATGGTTGACA CTCAGCTCAA GAGCGTTGGC GAGGAGCCCC GGGAGGTGCT GCTGCGGCTG 
TACGGGGCCA TCCTGCAGGG CGTGGACTCC TTGGTCCTTG AAAGTGTGAT GTTCGCCATA 
CTTGCAGAGC GGTCGCTGGG GCCCCAGCTC TACGGAGTCT TTCCAGAGGG CCGGCTGGAA 
CAGTACATCC CAAGCCGGCC ACTGAAGACA CATGAGCTTC GAGATCCAGT GTTGTCTGCA 
GCCATTGCCA CGAAGATGGC CAAGTTCCAC GGCATGGAGA TGCCTTTCAC TAAGGAGCCC 
CACTGGCTAT TCGGGACCAT GGAGCGGTAC TTAAAGCAGA TCCAGGACCT GCCCCCTACC 
AGCCTTCCCC AGATGAACCT GCTGGAGATG TACAGCTTGA AGGATGAGAT GGGCAACCTC 
AGGAAGCTGC TAGACACTAC CCCATCACCA GTGGTCTTTT GCCACAATGA TATCCAAGAA 
GGGAACATCT TACTGCTCTC AGAGCCTAAA AACACCGACA GCCTCATGCT GGTGGACTTC 
GAGTACAGCA GTTATAACTA CAGGGGCTTT GACATCGGGA ACCATTTTTG TGAGTGGGTT 
TACGATTATA CTCACGAGGA GTGGCCTTTC TACAAAGCGC AGCCTGCAAA CTACCCCACT 
GAGGGACAGC AGCTCCATTT TATTCGCCAC TACCTGGCAG AGGTAAAGAA AGATGAGACC 
ATCTCCCAAG AGGAGCAGAG GAAACTGGAA GCAGATTTGC TGGTGGAAGC TAATCGGTAT 
GCCCTGGCAT CTCATTTCTT TTGGGGTCTC TGGTCCATCC TTCAGGCATC CATGTCCACT 
ATAGAATTTG GTTACTTGGA GTATGCGCAG TCTCGGTTCC AGTTCTACTT CCAGCAGAAG 
GGCCAGATGA CCAGCTTCCA CCCCTCGTCC TGA