IRS1 cDNA ORF clone, Bison bison bison

Gene
Clones
gRNAs

Summary

Gene Symbol	IRS1
Entrez Gene ID	104986454
Full Name	insulin receptor substrate 1
Gene Type	protein-coding
Organism	Bison bison bison

mRNA and Protein(s)

mRNA	Protein	Name
XM_010837051.1	XP_010835353.1	insulin receptor substrate 1

Pathways from BioSystems
Gene Ontology

The following IRS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IRS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBi62600	XM_010837051.1 Latest version!	Bison bison bison insulin receptor substrate 1 (IRS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IRS1 ( XM_010837051.1 ) cDNA ORF clone, Bison bison bison -> XP_010835353.1 Bison bison bison insulin receptor substrate 1 (IRS1), mRNA.

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CloneID	OBi62600
Clone ID Related Accession (Same CDS sequence)	XM_010837051.1
Accession Version	XM_010837051.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	insulin receptor substrate 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011494640.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCC CTCCGGAGAC TGACGGCTTC TCGGACGTGC GCAAGGTGGG CTACCTGCGC 
AAACCCAAGA GCATGCACAA ACGATTCTTC GTGCTGCGGG CGGCCAGCGA GGCTGGGGGC 
CCGGCGCGCC TCGAGTACTA CGAGAACGAG AAGAAGTGGC GGCACAAGTC GAGCGCCCCC 
AAACGCTCGA TCCCCCTGGA GAGCTGCTTC AACATCAACA AGCGGGCGGA CTCCAAGAAC 
AAGCACCTGG TGGCCCTCTA CACCCGGGAC GAGCACTTTG CCATCGCGGC AGACAGCGAG 
GCCGAGCAGG ACAGCTGGTA CCAGGCCCTC CTGCAGCTGC ACAACCGTGC CAAGGGCCAC 
CACGACGGGG CCGCGGCGCC TGGGGCGGGA GGCGGCGGGG GCAGCTGCAG TGGCAGCTCC 
GGCCTCGGAG AGGCCGGTGA GGACTTGAGC TACGGGGACG TGCCCCCAGG ACCCGCCTTC 
AAGGAGGTCT GGCAGGTGAT CCTGAAACCC AAGGGCCTNN NNNNNNNNNN NNNNNNGATT 
GGCATCTACC GCCTCTGCCT GACCAGCAAG ACCATCAGCT TCGTGAAGCT GAACTCGGAG 
GCGGCGGCCG TGGTGCTGCA GCTGATGAAC ATCAGGCGCT GCGGCCACTC AGAGAACTTC 
TTCTTCATCG AAGTGGGCCG TTCCGCAGTG ACGGGCCCCG GGGAGTTCTG GATGCAGGTG 
GACGACTCCG TGGTGGCCCA GAACATGCAC GAGACAATCC TGGAGGCCAT GCGGGCCATG 
AGCGATGAGT TCCGCCCTAG AAGCAAGAGC CAGTCCTCCT CCAACTGCTC CAACCCCATC 
AGTGTGCCCC TGCGGAGGCA CCACCTCAAC AACCCTCCAC CCAGCCAGGT GGGGCTGACC 
CGCCGCTCGC GCACCGAGAG CATCACAGCC ACCTCCCCGG CCAGCTTGGT GGGCGGGAAG 
CAGGGCTCCT TCCGCGTGCG TGCCTCCAGT GATGGCGAAG GCACCATGTC TCGCCCCGCC 
TCAGTGGACG GCAGTCCTGT GAGTCCTAGC ACCAACAGGA CCCATGCCCA CCGCCATCGA 
GGCAGCTCCC GGCTGCACCC GCCTCTCAAC CACAGCCGCT CCATCCCCAT GCCTTCTTCT 
CGCTGCTCGC CTTCCGCCAC CAGCCCCGTC AGTCTGTCGT CCAGCAGCAC GAGTGGCCAT 
GGCTCCACCT CAGACTGCCT CTTCCCGCGG CGGTCTAGTG CTTCTGTGTC TGGTTCCCCC 
AGTGATGGCG GCTTCATCTC CTCCGATGAG TATGGCTCCA GTCCCTGCGA TTTCCGAAGT 
TCCTTCCGCA GCGTCACCCC AGATTCCCTG GGCCACACCC CGCCCGCCCG GGGTGAGGAG 
GAGCTAAGCA ACTACATCTG CATGGGAGGC AAGGGGGCCT CCACCCTCAC TGCCCCCAAT 
GGTCACTACA TTTTGCCCCG GGGTGGCAAT GGTCACCGCT ACGTGCCAGC CGCCGGCTTG 
GGCACGAGCC CAGCCCTGCC AGGAGAAGAA GCAGCCGGTG CGGCAGATCT GGATAATCGG 
TTCCGAAAAC GGACTCACTC TGCGGGCACC TCGCCTACCA TTTCCCACCA GAAAACCCCG 
TCTCAGTCCT CTGTGGCTTC CATTGAGGAA TATACCGAAA TGATGCCCGC TTACCCACCA 
GGAGGTGGCA GTGGAGGCCG AGTACCCAGC TACCGGCACT CCGCCTTCGT GCCCACCCAC 
TCCTACCCAG AGGAGGGTCT GGAAATGCAC CCCTTGGAGC GGCGTGGGGG CCACCACCGC 
CCAGACACCT CCAGCCTTCA CACTGATGAT GGCTACATGC CCATGTCCCC AGGGGTGGCC 
CCAGTGCCTG GCAGCCGGAA AGGCAGTGGG GACTACATGC CCATGAGCCC CAAGAGCGTG 
TCTGCCCCGC AGCAGATCAT CAACCCCATC AGACGCCATC CCCAGAGAGT GGACCCCAAC 
GGCTACATGA TGATGTCCCC AAGCGGCAGC TGCTCTCCTG ACATTGGAGG TGGGCCCAGC 
AGCGGTGGCA GCAGCAGCGG TGCCGCCCCT TCTGGGAGCA GCTATGGCAA GCTTTGGACA 
AACGGGGTAG GGGGCCACCA CTCTCACGCC CTGCCACACC CCAAACTTCC GGTGGAGAGC 
GGTAGTGGCA AACTCTTGTC TTGTACAGGT GACTACATGA ACATGTCGCC CGTCGGGGAC 
TCCAACACCA GCAGCCCCTC CGACTGCTAT TATGGCCCCG AGGACCCCCA GCACAAGCCC 
GTCCTCTCCT ACTACTCATT GCCAAGGTCC TTTAAGCACA CCCAGCGTCC TGGGGAGCTG 
GAGGAGCCCC GGCACCACCA GCACCTCCGC CTTTCCTCAA GTTCCGGTCG ACTCCTCTAC 
ACTGCGGCCG CAGAAGATTC CTCTTCGTCC ACCAGCAGCG ACAGCCTGGG CGGGGGATAC 
TGTGGGGCTC GGCCGGAGCC CGGCCTCCCG CATCTCCATC ATCAGGTCCT GCAGGCCCAT 
CTGCCTCGAA AGGTGGACAC AGCTGCCCAG ACCAACAACC GCCTGGCTCG GCCCACGAGG 
CTGTCTCTGG GGGATCCCAA GGCCAGCACC TTACCTCGGG CCCGAGAGCA GCAGCCACCG 
CCCCCCTTGC TGCTCCCTCC GGAGCCCAAG AGCCCAGGGG AGTATGTGAA TATTGAATTT 
GGGAGCGATC AGCCAGGCTA CTTATCGGGC CCGGTGGCGT CCCACAGCTC GCCTTCCATC 
AGGTGTCCGT CCCAGCTCCA GCCAGCTCCC AGAGAGGAGG ATACAGGCGC AGAAGAGTAT 
ATGAACATGG ACCTGGGGCC GGGCCGGAGG GCCACCTGGC AGAAGAGCGT GGGGGTCCAG 
CCCGGCAGGG TGGGCCCGGC GCCCCCTGGA GCTGCTAGCG TGTGCAGGCC GACTAGGGCA 
GTGCCCAGCA GCTGCGGCGA CTACATGACC ATGCAGATGG GTGGTCCCCG GCAGAGCTAC 
GTGGACACCT CACCTGTCGC GCCCATCAGC TACGCCGACA TGCGGACGGG CGTCGTTGTG 
GAGGATGCCA GCTTGCCCGG GGCCACCGCG GCCGCTCCCT CCTCATCTTC GACAGCCTCT 
GTTTCCTCCA CTGCGCCTCC TCCGGGAACA GGGGAGCTGG CGGCCCGCTC GGCCCTCCTG 
GGGGGCCCGA GCGCCTTCAC GCGGGTGAAC CTCAGTCCCA ACCGCAACCA GAGTGCCAAA 
GTGATCCGTG CCGACCCTCA AGGGTGCAGG AGGCGGCACA GCTCCGAGAC CTTCTCCTCT 
ACGCCCAGTG CCACCCGGGT GGGCAACACG ATGCCCTTCG GAGCTGGGGC TGCCGTCGGG 
GGCAGCGGTG GCGGCAGCAG CAGCAGCGCC GAGGATGTCA AACGCCACAG CTCTGCTTCC 
TTCGAGAACG TGTGGCTGAG GCCTGGGGAG CTCGGGGGAG CCCCCAAGGA GCTGGCCCAA 
GTGTGCGGGG CCGCTGGGGG TTTGGAGAAT GGTCTTAACT ACATAGACCT GGATTTGGTC 
AAGGACTTCA AACAGCGCCC TCAAGAGTGC CCACCTCAAC CGCAGCCTCC TCCACTCCCG 
GCCCCTCATC AGCCTCTGGC CAGTAGTCAG AGCGGCCCCC CCAGCCGCTC CAGCGAGGAT 
CTAAGCGCCT ATGCCAGCAT CACTTTCCAG AAGCAGCCAG AGGACCTCCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010835353.1
CDS	192..3905
Translation	MASPPETDGFSDVRKVGYLRKPKSMHKRFFVLRAASEAGGPARLEYYENEKKWRHKSSAPKRSIPLESCFNINKRADSKNKHLVALYTRDEHFAIAADSEAEQDSWYQALLQLHNRAKGHHDGAAAPGAGGGGGSCSGSSGLGEAGEDLSYGDVPPGPAFKEVWQVILKPKGnullnullnullnullnullnullnullIGIYRLCLTSKTISFVKLNSEAAAVVLQLMNIRRCGHSENFFFIEVGRSAVTGPGEFWMQVDDSVVAQNMHETILEAMRAMSDEFRPRSKSQSSSNCSNPISVPLRRHHLNNPPPSQVGLTRRSRTESITATSPASLVGGKQGSFRVRASSDGEGTMSRPASVDGSPVSPSTNRTHAHRHRGSSRLHPPLNHSRSIPMPSSRCSPSATSPVSLSSSSTSGHGSTSDCLFPRRSSASVSGSPSDGGFISSDEYGSSPCDFRSSFRSVTPDSLGHTPPARGEEELSNYICMGGKGASTLTAPNGHYILPRGGNGHRYVPAAGLGTSPALPGEEAAGAADLDNRFRKRTHSAGTSPTISHQKTPSQSSVASIEEYTEMMPAYPPGGGSGGRVPSYRHSAFVPTHSYPEEGLEMHPLERRGGHHRPDTSSLHTDDGYMPMSPGVAPVPGSRKGSGDYMPMSPKSVSAPQQIINPIRRHPQRVDPNGYMMMSPSGSCSPDIGGGPSSGGSSSGAAPSGSSYGKLWTNGVGGHHSHALPHPKLPVESGSGKLLSCTGDYMNMSPVGDSNTSSPSDCYYGPEDPQHKPVLSYYSLPRSFKHTQRPGELEEPRHHQHLRLSSSSGRLLYTAAAEDSSSSTSSDSLGGGYCGARPEPGLPHLHHQVLQAHLPRKVDTAAQTNNRLARPTRLSLGDPKASTLPRAREQQPPPPLLLPPEPKSPGEYVNIEFGSDQPGYLSGPVASHSSPSIRCPSQLQPAPREEDTGAEEYMNMDLGPGRRATWQKSVGVQPGRVGPAPPGAASVCRPTRAVPSSCGDYMTMQMGGPRQSYVDTSPVAPISYADMRTGVVVEDASLPGATAAAPSSSSTASVSSTAPPPGTGELAARSALLGGPSAFTRVNLSPNRNQSAKVIRADPQGCRRRHSSETFSSTPSATRVGNTMPFGAGAAVGGSGGGSSSSAEDVKRHSSASFENVWLRPGELGGAPKELAQVCGAAGGLENGLNYIDLDLVKDFKQRPQECPPQPQPPPLPAPHQPLASSQSGPPSRSSEDLSAYASITFQKQPEDLQ

Target ORF information:

RefSeq Version	XM_010837051.1
Organism	Bison bison bison
Definition	Bison bison bison insulin receptor substrate 1 (IRS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010837051.1

ORF Insert Sequence:

ATGGCGAGCC CTCCGGAGAC TGACGGCTTC TCGGACGTGC GCAAGGTGGG CTACCTGCGC 
AAACCCAAGA GCATGCACAA ACGATTCTTC GTGCTGCGGG CGGCCAGCGA GGCTGGGGGC 
CCGGCGCGCC TCGAGTACTA CGAGAACGAG AAGAAGTGGC GGCACAAGTC GAGCGCCCCC 
AAACGCTCGA TCCCCCTGGA GAGCTGCTTC AACATCAACA AGCGGGCGGA CTCCAAGAAC 
AAGCACCTGG TGGCCCTCTA CACCCGGGAC GAGCACTTTG CCATCGCGGC AGACAGCGAG 
GCCGAGCAGG ACAGCTGGTA CCAGGCCCTC CTGCAGCTGC ACAACCGTGC CAAGGGCCAC 
CACGACGGGG CCGCGGCGCC TGGGGCGGGA GGCGGCGGGG GCAGCTGCAG TGGCAGCTCC 
GGCCTCGGAG AGGCCGGTGA GGACTTGAGC TACGGGGACG TGCCCCCAGG ACCCGCCTTC 
AAGGAGGTCT GGCAGGTGAT CCTGAAACCC AAGGGCCTNN NNNNNNNNNN NNNNNNGATT 
GGCATCTACC GCCTCTGCCT GACCAGCAAG ACCATCAGCT TCGTGAAGCT GAACTCGGAG 
GCGGCGGCCG TGGTGCTGCA GCTGATGAAC ATCAGGCGCT GCGGCCACTC AGAGAACTTC 
TTCTTCATCG AAGTGGGCCG TTCCGCAGTG ACGGGCCCCG GGGAGTTCTG GATGCAGGTG 
GACGACTCCG TGGTGGCCCA GAACATGCAC GAGACAATCC TGGAGGCCAT GCGGGCCATG 
AGCGATGAGT TCCGCCCTAG AAGCAAGAGC CAGTCCTCCT CCAACTGCTC CAACCCCATC 
AGTGTGCCCC TGCGGAGGCA CCACCTCAAC AACCCTCCAC CCAGCCAGGT GGGGCTGACC 
CGCCGCTCGC GCACCGAGAG CATCACAGCC ACCTCCCCGG CCAGCTTGGT GGGCGGGAAG 
CAGGGCTCCT TCCGCGTGCG TGCCTCCAGT GATGGCGAAG GCACCATGTC TCGCCCCGCC 
TCAGTGGACG GCAGTCCTGT GAGTCCTAGC ACCAACAGGA CCCATGCCCA CCGCCATCGA 
GGCAGCTCCC GGCTGCACCC GCCTCTCAAC CACAGCCGCT CCATCCCCAT GCCTTCTTCT 
CGCTGCTCGC CTTCCGCCAC CAGCCCCGTC AGTCTGTCGT CCAGCAGCAC GAGTGGCCAT 
GGCTCCACCT CAGACTGCCT CTTCCCGCGG CGGTCTAGTG CTTCTGTGTC TGGTTCCCCC 
AGTGATGGCG GCTTCATCTC CTCCGATGAG TATGGCTCCA GTCCCTGCGA TTTCCGAAGT 
TCCTTCCGCA GCGTCACCCC AGATTCCCTG GGCCACACCC CGCCCGCCCG GGGTGAGGAG 
GAGCTAAGCA ACTACATCTG CATGGGAGGC AAGGGGGCCT CCACCCTCAC TGCCCCCAAT 
GGTCACTACA TTTTGCCCCG GGGTGGCAAT GGTCACCGCT ACGTGCCAGC CGCCGGCTTG 
GGCACGAGCC CAGCCCTGCC AGGAGAAGAA GCAGCCGGTG CGGCAGATCT GGATAATCGG 
TTCCGAAAAC GGACTCACTC TGCGGGCACC TCGCCTACCA TTTCCCACCA GAAAACCCCG 
TCTCAGTCCT CTGTGGCTTC CATTGAGGAA TATACCGAAA TGATGCCCGC TTACCCACCA 
GGAGGTGGCA GTGGAGGCCG AGTACCCAGC TACCGGCACT CCGCCTTCGT GCCCACCCAC 
TCCTACCCAG AGGAGGGTCT GGAAATGCAC CCCTTGGAGC GGCGTGGGGG CCACCACCGC 
CCAGACACCT CCAGCCTTCA CACTGATGAT GGCTACATGC CCATGTCCCC AGGGGTGGCC 
CCAGTGCCTG GCAGCCGGAA AGGCAGTGGG GACTACATGC CCATGAGCCC CAAGAGCGTG 
TCTGCCCCGC AGCAGATCAT CAACCCCATC AGACGCCATC CCCAGAGAGT GGACCCCAAC 
GGCTACATGA TGATGTCCCC AAGCGGCAGC TGCTCTCCTG ACATTGGAGG TGGGCCCAGC 
AGCGGTGGCA GCAGCAGCGG TGCCGCCCCT TCTGGGAGCA GCTATGGCAA GCTTTGGACA 
AACGGGGTAG GGGGCCACCA CTCTCACGCC CTGCCACACC CCAAACTTCC GGTGGAGAGC 
GGTAGTGGCA AACTCTTGTC TTGTACAGGT GACTACATGA ACATGTCGCC CGTCGGGGAC 
TCCAACACCA GCAGCCCCTC CGACTGCTAT TATGGCCCCG AGGACCCCCA GCACAAGCCC 
GTCCTCTCCT ACTACTCATT GCCAAGGTCC TTTAAGCACA CCCAGCGTCC TGGGGAGCTG 
GAGGAGCCCC GGCACCACCA GCACCTCCGC CTTTCCTCAA GTTCCGGTCG ACTCCTCTAC 
ACTGCGGCCG CAGAAGATTC CTCTTCGTCC ACCAGCAGCG ACAGCCTGGG CGGGGGATAC 
TGTGGGGCTC GGCCGGAGCC CGGCCTCCCG CATCTCCATC ATCAGGTCCT GCAGGCCCAT 
CTGCCTCGAA AGGTGGACAC AGCTGCCCAG ACCAACAACC GCCTGGCTCG GCCCACGAGG 
CTGTCTCTGG GGGATCCCAA GGCCAGCACC TTACCTCGGG CCCGAGAGCA GCAGCCACCG 
CCCCCCTTGC TGCTCCCTCC GGAGCCCAAG AGCCCAGGGG AGTATGTGAA TATTGAATTT 
GGGAGCGATC AGCCAGGCTA CTTATCGGGC CCGGTGGCGT CCCACAGCTC GCCTTCCATC 
AGGTGTCCGT CCCAGCTCCA GCCAGCTCCC AGAGAGGAGG ATACAGGCGC AGAAGAGTAT 
ATGAACATGG ACCTGGGGCC GGGCCGGAGG GCCACCTGGC AGAAGAGCGT GGGGGTCCAG 
CCCGGCAGGG TGGGCCCGGC GCCCCCTGGA GCTGCTAGCG TGTGCAGGCC GACTAGGGCA 
GTGCCCAGCA GCTGCGGCGA CTACATGACC ATGCAGATGG GTGGTCCCCG GCAGAGCTAC 
GTGGACACCT CACCTGTCGC GCCCATCAGC TACGCCGACA TGCGGACGGG CGTCGTTGTG 
GAGGATGCCA GCTTGCCCGG GGCCACCGCG GCCGCTCCCT CCTCATCTTC GACAGCCTCT 
GTTTCCTCCA CTGCGCCTCC TCCGGGAACA GGGGAGCTGG CGGCCCGCTC GGCCCTCCTG 
GGGGGCCCGA GCGCCTTCAC GCGGGTGAAC CTCAGTCCCA ACCGCAACCA GAGTGCCAAA 
GTGATCCGTG CCGACCCTCA AGGGTGCAGG AGGCGGCACA GCTCCGAGAC CTTCTCCTCT 
ACGCCCAGTG CCACCCGGGT GGGCAACACG ATGCCCTTCG GAGCTGGGGC TGCCGTCGGG 
GGCAGCGGTG GCGGCAGCAG CAGCAGCGCC GAGGATGTCA AACGCCACAG CTCTGCTTCC 
TTCGAGAACG TGTGGCTGAG GCCTGGGGAG CTCGGGGGAG CCCCCAAGGA GCTGGCCCAA 
GTGTGCGGGG CCGCTGGGGG TTTGGAGAAT GGTCTTAACT ACATAGACCT GGATTTGGTC 
AAGGACTTCA AACAGCGCCC TCAAGAGTGC CCACCTCAAC CGCAGCCTCC TCCACTCCCG 
GCCCCTCATC AGCCTCTGGC CAGTAGTCAG AGCGGCCCCC CCAGCCGCTC CAGCGAGGAT 
CTAAGCGCCT ATGCCAGCAT CACTTTCCAG AAGCAGCCAG AGGACCTCCA GTAG