TNF cDNA ORF clone, Bison bison bison

Gene
Clones
gRNAs

Summary

Gene Symbol	TNF
Entrez Gene ID	104983770
Full Name	tumor necrosis factor
Gene Type	protein-coding
Organism	Bison bison bison

mRNA and Protein(s)

mRNA	Protein	Name
XM_010833293.1	XP_010831595.1	tumor necrosis factor isoform X2
XM_010833292.1	XP_010831594.1	tumor necrosis factor isoform X1

Pathways from BioSystems
Gene Ontology

The following TNF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TNF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBi59861	XM_010833292.1 Latest version!	Bison bison bison tumor necrosis factor (TNF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
OBi59862	XM_010833293.1 Latest version!	Bison bison bison tumor necrosis factor (TNF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TNF ( XM_010833292.1 ) cDNA ORF clone, Bison bison bison -> XP_010831594.1 Bison bison bison tumor necrosis factor (TNF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBi59861
Clone ID Related Accession (Same CDS sequence)	XM_010833292.1
Accession Version	XM_010833292.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	tumor necrosis factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011493987.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACCA AAAGCATGAT CCGGGATGTG GAGCTGGCGG AGGAGGTGCT CTCCGAGAAA 
GCAGGGGGCC CCCAGGGCTC CAGAAGTTGC TTGTGCCTCA GCCTCTTCTC CTTCCTCCTG 
GTTGCAGGAG CCACCACGCT CTTCTGCCTG CTGCACTTCG GGGTAATCGG CCCCCAGAGG 
GAAGAGCAGT CCCCAGGTGG CCCCTCCATC AACAGCCCTC TGGTTCAGAC ACTCAGGTCC 
TCTTCTCAAG CCTCAAGTAA CAAGCCGGTA GCCCACGTTG TAGCCGACAT CAACTCTCCG 
GGGCAGCTCC GGTGGTGGGA TTCGTATGCC AATGCCCTCA TGGCCAACGG TGTGAAGCTG 
GAAGACAACC AGCTGGTGGT GCCTGCTGAC GGGCTTTACC TCATCTACTC ACAGGTCCTC 
TTCAGGGGCC AAGGCTGCCC TTCCACCCCC TTGTTCCTCA CCCACACCAT CAGCCGCATT 
GCAGTCTCCT ACCAGACCAA GGTCAACATC CTGTCTGCCA TCAAGAGCCC TTGCCACAGG 
GAGACCCCAG AGTGGGCTGA GGCCAAGCCC TGGTACGAAC CCATCTACCA GGGAGGAGTC 
TTCCAGCTGG AGAAGGGAGA TCGCCTCAGT GCTGAGATCA ACCTGCCGGA CTACCTGGAC 
TATGCCGAGT CTGGGCAGGT CTACTTTGGG ATCATCGCCC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010831594.1
CDS	247..951
Translation	MSTKSMIRDVELAEEVLSEKAGGPQGSRSCLCLSLFSFLLVAGATTLFCLLHFGVIGPQREEQSPGGPSINSPLVQTLRSSSQASSNKPVAHVVADINSPGQLRWWDSYANALMANGVKLEDNQLVVPADGLYLIYSQVLFRGQGCPSTPLFLTHTISRIAVSYQTKVNILSAIKSPCHRETPEWAEAKPWYEPIYQGGVFQLEKGDRLSAEINLPDYLDYAESGQVYFGIIAL

Target ORF information:

RefSeq Version	XM_010833292.1
Organism	Bison bison bison
Definition	Bison bison bison tumor necrosis factor (TNF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010833292.1

ORF Insert Sequence:

ATGAGCACCA AAAGCATGAT CCGGGATGTG GAGCTGGCGG AGGAGGTGCT CTCCGAGAAA 
GCAGGGGGCC CCCAGGGCTC CAGAAGTTGC TTGTGCCTCA GCCTCTTCTC CTTCCTCCTG 
GTTGCAGGAG CCACCACGCT CTTCTGCCTG CTGCACTTCG GGGTAATCGG CCCCCAGAGG 
GAAGAGCAGT CCCCAGGTGG CCCCTCCATC AACAGCCCTC TGGTTCAGAC ACTCAGGTCC 
TCTTCTCAAG CCTCAAGTAA CAAGCCGGTA GCCCACGTTG TAGCCGACAT CAACTCTCCG 
GGGCAGCTCC GGTGGTGGGA TTCGTATGCC AATGCCCTCA TGGCCAACGG TGTGAAGCTG 
GAAGACAACC AGCTGGTGGT GCCTGCTGAC GGGCTTTACC TCATCTACTC ACAGGTCCTC 
TTCAGGGGCC AAGGCTGCCC TTCCACCCCC TTGTTCCTCA CCCACACCAT CAGCCGCATT 
GCAGTCTCCT ACCAGACCAA GGTCAACATC CTGTCTGCCA TCAAGAGCCC TTGCCACAGG 
GAGACCCCAG AGTGGGCTGA GGCCAAGCCC TGGTACGAAC CCATCTACCA GGGAGGAGTC 
TTCCAGCTGG AGAAGGGAGA TCGCCTCAGT GCTGAGATCA ACCTGCCGGA CTACCTGGAC 
TATGCCGAGT CTGGGCAGGT CTACTTTGGG ATCATCGCCC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNF ( XM_010833293.1 ) cDNA ORF clone, Bison bison bison -> XP_010831595.1 Bison bison bison tumor necrosis factor (TNF), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBi59862
Clone ID Related Accession (Same CDS sequence)	XM_010833293.1
Accession Version	XM_010833293.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	tumor necrosis factor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011493987.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACCA AAAGCATGAT CCGGGATGTG GAGCTGGCGG AGGAGGTGCT CTCCGAGAAA 
GCAGGGGGCC CCCAGGGCTC CAGAAGTTGC TTGTGCCTCA GCCTCTTCTC CTTCCTCCTG 
GTTGCAGGAG CCACCACGCT CTTCTGCCTG CTGCACTTCG GGGTAATCGG CCCCCAGAGG 
GAAGAGTCCC CAGGTGGCCC CTCCATCAAC AGCCCTCTGG TTCAGACACT CAGGTCCTCT 
TCTCAAGCCT CAAGTAACAA GCCGGTAGCC CACGTTGTAG CCGACATCAA CTCTCCGGGG 
CAGCTCCGGT GGTGGGATTC GTATGCCAAT GCCCTCATGG CCAACGGTGT GAAGCTGGAA 
GACAACCAGC TGGTGGTGCC TGCTGACGGG CTTTACCTCA TCTACTCACA GGTCCTCTTC 
AGGGGCCAAG GCTGCCCTTC CACCCCCTTG TTCCTCACCC ACACCATCAG CCGCATTGCA 
GTCTCCTACC AGACCAAGGT CAACATCCTG TCTGCCATCA AGAGCCCTTG CCACAGGGAG 
ACCCCAGAGT GGGCTGAGGC CAAGCCCTGG TACGAACCCA TCTACCAGGG AGGAGTCTTC 
CAGCTGGAGA AGGGAGATCG CCTCAGTGCT GAGATCAACC TGCCGGACTA CCTGGACTAT 
GCCGAGTCTG GGCAGGTCTA CTTTGGGATC ATCGCCCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010831595.1
CDS	33..734
Translation	MSTKSMIRDVELAEEVLSEKAGGPQGSRSCLCLSLFSFLLVAGATTLFCLLHFGVIGPQREESPGGPSINSPLVQTLRSSSQASSNKPVAHVVADINSPGQLRWWDSYANALMANGVKLEDNQLVVPADGLYLIYSQVLFRGQGCPSTPLFLTHTISRIAVSYQTKVNILSAIKSPCHRETPEWAEAKPWYEPIYQGGVFQLEKGDRLSAEINLPDYLDYAESGQVYFGIIAL

Target ORF information:

RefSeq Version	XM_010833293.1
Organism	Bison bison bison
Definition	Bison bison bison tumor necrosis factor (TNF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010833293.1

ORF Insert Sequence:

ATGAGCACCA AAAGCATGAT CCGGGATGTG GAGCTGGCGG AGGAGGTGCT CTCCGAGAAA 
GCAGGGGGCC CCCAGGGCTC CAGAAGTTGC TTGTGCCTCA GCCTCTTCTC CTTCCTCCTG 
GTTGCAGGAG CCACCACGCT CTTCTGCCTG CTGCACTTCG GGGTAATCGG CCCCCAGAGG 
GAAGAGTCCC CAGGTGGCCC CTCCATCAAC AGCCCTCTGG TTCAGACACT CAGGTCCTCT 
TCTCAAGCCT CAAGTAACAA GCCGGTAGCC CACGTTGTAG CCGACATCAA CTCTCCGGGG 
CAGCTCCGGT GGTGGGATTC GTATGCCAAT GCCCTCATGG CCAACGGTGT GAAGCTGGAA 
GACAACCAGC TGGTGGTGCC TGCTGACGGG CTTTACCTCA TCTACTCACA GGTCCTCTTC 
AGGGGCCAAG GCTGCCCTTC CACCCCCTTG TTCCTCACCC ACACCATCAG CCGCATTGCA 
GTCTCCTACC AGACCAAGGT CAACATCCTG TCTGCCATCA AGAGCCCTTG CCACAGGGAG 
ACCCCAGAGT GGGCTGAGGC CAAGCCCTGG TACGAACCCA TCTACCAGGG AGGAGTCTTC 
CAGCTGGAGA AGGGAGATCG CCTCAGTGCT GAGATCAACC TGCCGGACTA CCTGGACTAT 
GCCGAGTCTG GGCAGGTCTA CTTTGGGATC ATCGCCCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.