BRPF3 cDNA ORF clone, Balearica regulorum gibbericeps(East African grey crowned-crane)

Gene
Clones
gRNAs

Summary

Gene Symbol	BRPF3
Entrez Gene ID	104635688
Full Name	bromodomain and PHD finger containing 3
Gene Type	protein-coding
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010302579.1	XP_010300881.1	bromodomain and PHD finger-containing protein 3 isoform X2
XM_010302578.1	XP_010300880.1	bromodomain and PHD finger-containing protein 3 isoform X1

Pathways from BioSystems
Gene Ontology

The following BRPF3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRPF3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa186716	XM_010302578.1 Latest version!	Balearica regulorum gibbericeps bromodomain and PHD finger containing, 3 (BRPF3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa186717	XM_010302579.1 Latest version!	Balearica regulorum gibbericeps bromodomain and PHD finger containing, 3 (BRPF3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRPF3 ( XM_010302578.1 ) cDNA ORF clone, Balearica regulorum gibbericeps(East African grey crowned-crane) -> XP_010300880.1 Balearica regulorum gibbericeps bromodomain and PHD finger containing, 3 (BRPF3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa186716
Clone ID Related Accession (Same CDS sequence)	XM_010302578.1
Accession Version	XM_010302578.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-17
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)
Product	bromodomain and PHD finger-containing protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010755776.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Balearica regulorum gibbericeps Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAAAC CTCGGAGGAG GTCCCGGCAG AACTTGGAGG GGAGACGTTC TCCCTCACCC 
TACACCCTCA AGTGCTCACC GACTCGGGAG ACACTGACGT ACGCTCAGGC CCAGCGGATC 
GTGGAGGTAG ACATCGATGG GCGTCTTCAT CGCATCAGCA TCTACGATCC CTTAAAAATC 
ATCACAGAGG ATGAGCTGAC AGCCCAGGAC ATCACAGAGT GCAACAGCAA CAAGGAAAAC 
AGCGAGCAGC CCCTTTTCCC TTCCAAATCT AAGAAAACAC CTTCCAAAGG CAAGAAGAAG 
GAGTCCTGCT CCAAACATGC ACCAGGGACA TCTTTACACT TACCCCAGCC CAACTTCCGT 
GTGGTGGACT CTTTCAGTCA GCCACATGCT CCTCCTCTCC CCGCTGCCTA CTACCGGTAC 
ATTGAAAAGC CTCCCGAGGA CCTTGATGTA GAGGTGGAGT ATGACATGGA TGAGGAGGAT 
CTGGCCTGGC TGGAAATAGT CAACGAGAAG AGAAGGGATG ATGGTTATGG GACAGTTAGT 
GCTGACACTT TTGAGCTGCT GGTGGACCGG TTGGAAAAGG AGTCGTACCT CGAGAGCCGG 
AACAACGGGG CTCAGCAGTC TGTCATCGAT GAGGATGCCT TCTGCTGCGT CTGCATGGAT 
GATGAGTGTC ACAACAGCAA CGTCATCCTG TTCTGTGATA TCTGCAATCT GGCTGTGCAC 
CAGGAGTGTT ACGGCGTGCC CTATATCCCT GAGGGGCAGT GGCTTTGCCG CTGCTGCTTA 
CAGTCCCCTT CTCGTCCTGT GGATTGTGTC CTTTGCCCGA ACAAAGGTGG AGCCTTCAAG 
CAGACCAGCG ATGGCCGCTG GGCTCATGTG GTTTGTGCCA TCTGGATCCC AGAGGTCTGC 
TTTGCAAACA CGGTGTTCCT GGAGCCCATT GAAGGGATAA ATAACATCCC CCCGGCTCGG 
TGGAAGCTCA CATGCTATAT CTGCAAGCAG AAGGGCATGG GAGCTGCTAT CCAATGCCAC 
AAGGTGAACT GTTATGCTGC CTTCCATGTC ACCTGTGCCC AGCGGGCTGG CCTCTTCATG 
AAGATCGAAC CCATGAGGGA GACCAGCATC AATGGCACAA CGTTCACCGT GCGCAAGACT 
GCCTATTGTG AGAGTCATTC CCCACCTGGA ACCGTGAAAA AAGGGTCTCC AGCTGCTAGT 
GGTGAGGGAC AGGAGGACAC TGTGAAAGAG GAGGGGGAGG ACAAAGCCAA TTGCAGCCCT 
CCCAAAGGGT CTCTGAAAAA GAACCAAGTG AAATTGAAGC AGAAGATCAA AAAGGAGCCT 
GGTGCTGTGA CCAATGGGCG CTCGTCTATG CCTATGGTGA CAGTCGCACA AATCCCCTCT 
TACAGGCTGA ACAAAATCTG CCACGGCCTT TCCCTCCAGA GGAAGAACCA GTTCATGAGG 
AGGCTCCACA ACTACTGGCT GCTGAAGCGA CAGGCGAGGA ACGGGGTGCC CCTGATCCGG 
CGCCTGCACT CACATCTCCA GTCTCAGAGG AACGCGGAGC AGAAGGAGCA GGATGAGAAG 
ACCAGTGCTG TGAAGGAAGA GCTGAAGTAC TGGCAAAAGC TGCGGCATGA CTTGGAGCGG 
GCACGGCTGC TCATCGAGCT GATCCGTAAG AGGGAAAAAC TCAAACGGGA GCAGGTCAAG 
TGTCAGCAGG CTGCAATGGA GCTACAGCTG ACCCCTTTCA ATGTACTTCT ACGCACAACT 
CTGGACCTGC TGCAGGAGAA GGATGCTGCC CAGATCTTTG CAGAGCCTGT TAACCTAAAT 
GAGGTTCCAG ATTACCTGGA ATTCATTTCC AACCCAATGG ATTTTTCCAC CATGAGGCGG 
AAGCTGGAGT CCCACCTGTA CCGAACATTG GATGAGTTTG AGGAAGACTT TAACCTTATA 
GTTACCAACT GCATGAGGTA TAATGCTAAA GACACGATTT TCCACCGAGC AGCTGTCCGG 
CTCAGAGACC TTGGAGGAGC GATATTGCGT CATGCACGGC GGCAGGCTGA AAACATCGGC 
TTTGACACTG ATGTGGGGAT TCACCTGCCC GAGTCACCCA AAACCGAGGA CTTTTACCGC 
TTTTCTTGGG AGGATGTGGA TAACATCCTC CTCCCAGAGA ACCGGGCTCA TCTCTCCTTG 
GAGGCCCAGC TGAAGGAGCT GCTGGAGAAG CTAGACATGG TGAGCACCAT GCGATCCAGC 
GGTGCCCGGA CACGACGCAT CCGGCTCCTG AGACGGGAGA TCAACTCCAT TCGGCAGAAA 
CTGGCCCAGC AGCAGAACAA AACCATGCCC AACGGGGAGC CTGTGCCGTG GGAAGAGGGG 
CTGGATAAAG CATCGAGGGA AGGGGATGAG GAAGGGGATA AAGCAGATGA TGCCAAGCCC 
CCGCACCCTC CAACCCTGGA GCCCACAGGA CCTGCGCCCT CTCTCTCAGA GCTGGACTCT 
CTGCAGGACC CTCCAATGCT CAAACCCATC AGTGAAAGCA AGTCCTTGAA TCAGCTGCAG 
AAGAGGGTGA TGCCGGACAG GGAGCTCTTT GACAAGAAGG CTCTGCAGCG GGAGAGCCAA 
GCCTTCCAGC GCCTGCTCAG CGACAACGGC CTGAACGGAC TGGCCTTGCC GCCTGCAGAC 
ACCCCAGCCA GCCCCCCGTT CAGTGGTGTG GGCCGACGGA CATCTGTCCT TTTTAAAAAA 
GCTAAGAACG GGGTGAAGCT GCAAAGAGGC CTGGACTGCT CCCTGGAGAA TGGGGAGGAC 
CATGGGCAGA GTGGGCAGCT TTCACCCTCG CGCCCTGATG GGGAACGACA AACTCGGAAG 
CGGCCGCAGA GCAGGACCGG CAGCGAGAGC GATGGAGAGA AGTCGCCCAG GCAGGCAGGA 
CAGAGAGGAG TGACTAACGG CTTTGTGAAG CATGCAGAAA GCAGCTCTGA CTCTGAGTGT 
AGCACTGGCC GGGGCGGTGC GCTGGTGTTT GAAGCCTGCA GTGGTTTGAT GCCTCCCAAG 
CGAAGTCGAG GCAAGCCAGC TCTTTCTCGG ATGCCCTTCT TGGAAGGCGT GAATGGAGAC 
TCCGATTATG GCAGCTCAGG CAGGACTCTC CTGATGTCCT TTGAGAGTCA GGCTGAGCTG 
GAGCCCTTGG AGCTTGTGTG GGCCAAGTGC CGTGGCTATC CCTCCTACCC TGCCTTGATA 
ATCGATCCTA AGATGCCGCG CGAGGGTTTG CTCCACAATG GAGTCCCCAT CCCAGTCCCA 
CCCATGGATG TGTTGAAGCT GGGGGAGCAG AGGCAGACAG AGGCAGGAGA AAAGCTCTTC 
CTTGTCCTTT TCTTTGACAA CAAGAGAACC TGGCAGTGGC TTCCGCGTGA CAAAGTCTAC 
CCCCTCGGCG TGGACGACAC AGTGGACAAG TTAAAAATGA TGGAAGGCCG AAAAACCAGC 
ATCCGCAAGT CTGTGCAAGT GGCATATGAC CGAGCCATGA TCCACATGAG CCGTGTGCGG 
GGAGATCATC CCTTCGTTAC GTCCAGCTAT CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010300880.1
CDS	1..3516
Translation	MRKPRRRSRQNLEGRRSPSPYTLKCSPTRETLTYAQAQRIVEVDIDGRLHRISIYDPLKIITEDELTAQDITECNSNKENSEQPLFPSKSKKTPSKGKKKESCSKHAPGTSLHLPQPNFRVVDSFSQPHAPPLPAAYYRYIEKPPEDLDVEVEYDMDEEDLAWLEIVNEKRRDDGYGTVSADTFELLVDRLEKESYLESRNNGAQQSVIDEDAFCCVCMDDECHNSNVILFCDICNLAVHQECYGVPYIPEGQWLCRCCLQSPSRPVDCVLCPNKGGAFKQTSDGRWAHVVCAIWIPEVCFANTVFLEPIEGINNIPPARWKLTCYICKQKGMGAAIQCHKVNCYAAFHVTCAQRAGLFMKIEPMRETSINGTTFTVRKTAYCESHSPPGTVKKGSPAASGEGQEDTVKEEGEDKANCSPPKGSLKKNQVKLKQKIKKEPGAVTNGRSSMPMVTVAQIPSYRLNKICHGLSLQRKNQFMRRLHNYWLLKRQARNGVPLIRRLHSHLQSQRNAEQKEQDEKTSAVKEELKYWQKLRHDLERARLLIELIRKREKLKREQVKCQQAAMELQLTPFNVLLRTTLDLLQEKDAAQIFAEPVNLNEVPDYLEFISNPMDFSTMRRKLESHLYRTLDEFEEDFNLIVTNCMRYNAKDTIFHRAAVRLRDLGGAILRHARRQAENIGFDTDVGIHLPESPKTEDFYRFSWEDVDNILLPENRAHLSLEAQLKELLEKLDMVSTMRSSGARTRRIRLLRREINSIRQKLAQQQNKTMPNGEPVPWEEGLDKASREGDEEGDKADDAKPPHPPTLEPTGPAPSLSELDSLQDPPMLKPISESKSLNQLQKRVMPDRELFDKKALQRESQAFQRLLSDNGLNGLALPPADTPASPPFSGVGRRTSVLFKKAKNGVKLQRGLDCSLENGEDHGQSGQLSPSRPDGERQTRKRPQSRTGSESDGEKSPRQAGQRGVTNGFVKHAESSSDSECSTGRGGALVFEACSGLMPPKRSRGKPALSRMPFLEGVNGDSDYGSSGRTLLMSFESQAELEPLELVWAKCRGYPSYPALIIDPKMPREGLLHNGVPIPVPPMDVLKLGEQRQTEAGEKLFLVLFFDNKRTWQWLPRDKVYPLGVDDTVDKLKMMEGRKTSIRKSVQVAYDRAMIHMSRVRGDHPFVTSSYL

Target ORF information:

RefSeq Version	XM_010302578.1
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)
Definition	Balearica regulorum gibbericeps bromodomain and PHD finger containing, 3 (BRPF3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010302578.1

ORF Insert Sequence:

ATGAGGAAAC CTCGGAGGAG GTCCCGGCAG AACTTGGAGG GGAGACGTTC TCCCTCACCC 
TACACCCTCA AGTGCTCACC GACTCGGGAG ACACTGACGT ACGCTCAGGC CCAGCGGATC 
GTGGAGGTAG ACATCGATGG GCGTCTTCAT CGCATCAGCA TCTACGATCC CTTAAAAATC 
ATCACAGAGG ATGAGCTGAC AGCCCAGGAC ATCACAGAGT GCAACAGCAA CAAGGAAAAC 
AGCGAGCAGC CCCTTTTCCC TTCCAAATCT AAGAAAACAC CTTCCAAAGG CAAGAAGAAG 
GAGTCCTGCT CCAAACATGC ACCAGGGACA TCTTTACACT TACCCCAGCC CAACTTCCGT 
GTGGTGGACT CTTTCAGTCA GCCACATGCT CCTCCTCTCC CCGCTGCCTA CTACCGGTAC 
ATTGAAAAGC CTCCCGAGGA CCTTGATGTA GAGGTGGAGT ATGACATGGA TGAGGAGGAT 
CTGGCCTGGC TGGAAATAGT CAACGAGAAG AGAAGGGATG ATGGTTATGG GACAGTTAGT 
GCTGACACTT TTGAGCTGCT GGTGGACCGG TTGGAAAAGG AGTCGTACCT CGAGAGCCGG 
AACAACGGGG CTCAGCAGTC TGTCATCGAT GAGGATGCCT TCTGCTGCGT CTGCATGGAT 
GATGAGTGTC ACAACAGCAA CGTCATCCTG TTCTGTGATA TCTGCAATCT GGCTGTGCAC 
CAGGAGTGTT ACGGCGTGCC CTATATCCCT GAGGGGCAGT GGCTTTGCCG CTGCTGCTTA 
CAGTCCCCTT CTCGTCCTGT GGATTGTGTC CTTTGCCCGA ACAAAGGTGG AGCCTTCAAG 
CAGACCAGCG ATGGCCGCTG GGCTCATGTG GTTTGTGCCA TCTGGATCCC AGAGGTCTGC 
TTTGCAAACA CGGTGTTCCT GGAGCCCATT GAAGGGATAA ATAACATCCC CCCGGCTCGG 
TGGAAGCTCA CATGCTATAT CTGCAAGCAG AAGGGCATGG GAGCTGCTAT CCAATGCCAC 
AAGGTGAACT GTTATGCTGC CTTCCATGTC ACCTGTGCCC AGCGGGCTGG CCTCTTCATG 
AAGATCGAAC CCATGAGGGA GACCAGCATC AATGGCACAA CGTTCACCGT GCGCAAGACT 
GCCTATTGTG AGAGTCATTC CCCACCTGGA ACCGTGAAAA AAGGGTCTCC AGCTGCTAGT 
GGTGAGGGAC AGGAGGACAC TGTGAAAGAG GAGGGGGAGG ACAAAGCCAA TTGCAGCCCT 
CCCAAAGGGT CTCTGAAAAA GAACCAAGTG AAATTGAAGC AGAAGATCAA AAAGGAGCCT 
GGTGCTGTGA CCAATGGGCG CTCGTCTATG CCTATGGTGA CAGTCGCACA AATCCCCTCT 
TACAGGCTGA ACAAAATCTG CCACGGCCTT TCCCTCCAGA GGAAGAACCA GTTCATGAGG 
AGGCTCCACA ACTACTGGCT GCTGAAGCGA CAGGCGAGGA ACGGGGTGCC CCTGATCCGG 
CGCCTGCACT CACATCTCCA GTCTCAGAGG AACGCGGAGC AGAAGGAGCA GGATGAGAAG 
ACCAGTGCTG TGAAGGAAGA GCTGAAGTAC TGGCAAAAGC TGCGGCATGA CTTGGAGCGG 
GCACGGCTGC TCATCGAGCT GATCCGTAAG AGGGAAAAAC TCAAACGGGA GCAGGTCAAG 
TGTCAGCAGG CTGCAATGGA GCTACAGCTG ACCCCTTTCA ATGTACTTCT ACGCACAACT 
CTGGACCTGC TGCAGGAGAA GGATGCTGCC CAGATCTTTG CAGAGCCTGT TAACCTAAAT 
GAGGTTCCAG ATTACCTGGA ATTCATTTCC AACCCAATGG ATTTTTCCAC CATGAGGCGG 
AAGCTGGAGT CCCACCTGTA CCGAACATTG GATGAGTTTG AGGAAGACTT TAACCTTATA 
GTTACCAACT GCATGAGGTA TAATGCTAAA GACACGATTT TCCACCGAGC AGCTGTCCGG 
CTCAGAGACC TTGGAGGAGC GATATTGCGT CATGCACGGC GGCAGGCTGA AAACATCGGC 
TTTGACACTG ATGTGGGGAT TCACCTGCCC GAGTCACCCA AAACCGAGGA CTTTTACCGC 
TTTTCTTGGG AGGATGTGGA TAACATCCTC CTCCCAGAGA ACCGGGCTCA TCTCTCCTTG 
GAGGCCCAGC TGAAGGAGCT GCTGGAGAAG CTAGACATGG TGAGCACCAT GCGATCCAGC 
GGTGCCCGGA CACGACGCAT CCGGCTCCTG AGACGGGAGA TCAACTCCAT TCGGCAGAAA 
CTGGCCCAGC AGCAGAACAA AACCATGCCC AACGGGGAGC CTGTGCCGTG GGAAGAGGGG 
CTGGATAAAG CATCGAGGGA AGGGGATGAG GAAGGGGATA AAGCAGATGA TGCCAAGCCC 
CCGCACCCTC CAACCCTGGA GCCCACAGGA CCTGCGCCCT CTCTCTCAGA GCTGGACTCT 
CTGCAGGACC CTCCAATGCT CAAACCCATC AGTGAAAGCA AGTCCTTGAA TCAGCTGCAG 
AAGAGGGTGA TGCCGGACAG GGAGCTCTTT GACAAGAAGG CTCTGCAGCG GGAGAGCCAA 
GCCTTCCAGC GCCTGCTCAG CGACAACGGC CTGAACGGAC TGGCCTTGCC GCCTGCAGAC 
ACCCCAGCCA GCCCCCCGTT CAGTGGTGTG GGCCGACGGA CATCTGTCCT TTTTAAAAAA 
GCTAAGAACG GGGTGAAGCT GCAAAGAGGC CTGGACTGCT CCCTGGAGAA TGGGGAGGAC 
CATGGGCAGA GTGGGCAGCT TTCACCCTCG CGCCCTGATG GGGAACGACA AACTCGGAAG 
CGGCCGCAGA GCAGGACCGG CAGCGAGAGC GATGGAGAGA AGTCGCCCAG GCAGGCAGGA 
CAGAGAGGAG TGACTAACGG CTTTGTGAAG CATGCAGAAA GCAGCTCTGA CTCTGAGTGT 
AGCACTGGCC GGGGCGGTGC GCTGGTGTTT GAAGCCTGCA GTGGTTTGAT GCCTCCCAAG 
CGAAGTCGAG GCAAGCCAGC TCTTTCTCGG ATGCCCTTCT TGGAAGGCGT GAATGGAGAC 
TCCGATTATG GCAGCTCAGG CAGGACTCTC CTGATGTCCT TTGAGAGTCA GGCTGAGCTG 
GAGCCCTTGG AGCTTGTGTG GGCCAAGTGC CGTGGCTATC CCTCCTACCC TGCCTTGATA 
ATCGATCCTA AGATGCCGCG CGAGGGTTTG CTCCACAATG GAGTCCCCAT CCCAGTCCCA 
CCCATGGATG TGTTGAAGCT GGGGGAGCAG AGGCAGACAG AGGCAGGAGA AAAGCTCTTC 
CTTGTCCTTT TCTTTGACAA CAAGAGAACC TGGCAGTGGC TTCCGCGTGA CAAAGTCTAC 
CCCCTCGGCG TGGACGACAC AGTGGACAAG TTAAAAATGA TGGAAGGCCG AAAAACCAGC 
ATCCGCAAGT CTGTGCAAGT GGCATATGAC CGAGCCATGA TCCACATGAG CCGTGTGCGG 
GGAGATCATC CCTTCGTTAC GTCCAGCTAT CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRPF3 ( XM_010302579.1 ) cDNA ORF clone, Balearica regulorum gibbericeps(East African grey crowned-crane) -> XP_010300881.1 Balearica regulorum gibbericeps bromodomain and PHD finger containing, 3 (BRPF3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa186717
Clone ID Related Accession (Same CDS sequence)	XM_010302579.1
Accession Version	XM_010302579.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-17
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)
Product	bromodomain and PHD finger-containing protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010755776.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Balearica regulorum gibbericeps Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAAAC CTCGGAGGAG GTCCCGGCAG AACTTGGAGG GGAGACGTTC TCCCTCACCC 
TACACCCTCA AGTGCTCACC GACTCGGGAG ACACTGACGT ACGCTCAGGC CCAGCGGATC 
GTGGAGGTAG ACATCGATGG GCGTCTTCAT CGCATCAGCA TCTACGATCC CTTAAAAATC 
ATCACAGAGG ATGAGCTGAC AGCCCAGGAC ATCACAGAGT GCAACAGCAA CAAGGAAAAC 
AGCGAGCAGC CCCTTTTCCC TTCCAAATCT AAGAAAACAC CTTCCAAAGG CAAGAAGAAG 
GAGTCCTGCT CCAAACATGC ACCAGGGACA TCTTTACACT TACCCCAGCC CAACTTCCGT 
GTGGTGGACT CTTTCAGTCA GCCACATGCT CCTCCTCTCC CCGCTGCCTA CTACCGGTAC 
ATTGAAAAGC CTCCCGAGGA CCTTGATGTA GAGGTGGAGT ATGACATGGA TGAGGAGGAT 
CTGGCCTGGC TGGAAATAGT CAACGAGAAG AGAAGGGATG ATGGTTATGG GACAGTTAGT 
GCTGACACTT TTGAGCTGCT GGTGGACCGG TTGGAAAAGG AGTCGTACCT CGAGAGCCGG 
AACAACGGGG CTCAGCAGTC TGTCATCGAT GAGGATGCCT TCTGCTGCGT CTGCATGGAT 
GATGAGTGTC ACAACAGCAA CGTCATCCTG TTCTGTGATA TCTGCAATCT GGCTGTGCAC 
CAGGAGTGTT ACGGCGTGCC CTATATCCCT GAGGGGCAGT GGCTTTGCCG CTGCTGCTTA 
CAGTCCCCTT CTCGTCCTGT GGATTGTGTC CTTTGCCCGA ACAAAGGTGG AGCCTTCAAG 
CAGACCAGCG ATGGCCGCTG GGCTCATGTG GTTTGTGCCA TCTGGATCCC AGAGGTCTGC 
TTTGCAAACA CGGTGTTCCT GGAGCCCATT GAAGGGATAA ATAACATCCC CCCGGCTCGG 
TGGAAGCTCA CATGCTATAT CTGCAAGCAG AAGGGCATGG GAGCTGCTAT CCAATGCCAC 
AAGGTGAACT GTTATGCTGC CTTCCATGTC ACCTGTGCCC AGCGGGCTGG CCTCTTCATG 
AAGATCGAAC CCATGAGGGA GACCAGCATC AATGGCACAA CGTTCACCGT GCGCAAGACT 
GCCTATTGTG AGAGTCATTC CCCACCTGGA ACCGTGAAAA AAGGGTCTCC AGCTGCTAGT 
GGTGAGGGAC AGGAGGACAC TGTGAAAGAG GAGGGGGAGG ACAAAGCCAA TTGCAGCCCT 
CCCAAAGGGT CTCTGAAAAA GAACCAAGTG AAATTGAAGC AGAAGATCAA AAAGGAGCCT 
GGTGCTGTGA CCAATGGGCG CTCGTCTATG CCTATGGTGA CAGTCGCACA AATCCCCTCT 
TACAGGCTGA ACAAAATCTG CCACGGCCTT TCCCTCCAGA GGAAGAACCA GTTCATGAGG 
AGGCTCCACA ACTACTGGCT GCTGAAGCGA CAGGCGAGGA ACGGGGTGCC CCTGATCCGG 
CGCCTGCACT CACATCTCCA GTCTCAGAGG AACGCGGAGC AGAAGGAGCA GGATGAGAAG 
ACCAGTGCTG TGAAGGAAGA GCTGAAGTAC TGGCAAAAGC TGCGGCATGA CTTGGAGCGG 
GCACGGCTGC TCATCGAGCT GATCCGTAAG AGGGAAAAAC TCAAACGGGA GCAGGTCAAG 
TGTCAGCAGG CTGCAATGGA GCTACAGCTG ACCCCTTTCA ATGTACTTCT ACGCACAACT 
CTGGACCTGC TGCAGGAGAA GGATGCTGCC CAGATCTTTG CAGAGCCTGT TAACCTAAAT 
GAGGTTCCAG ATTACCTGGA ATTCATTTCC AACCCAATGG ATTTTTCCAC CATGAGGCGG 
AAGCTGGAGT CCCACCTGTA CCGAACATTG GATGAGTTTG AGGAAGACTT TAACCTTATA 
GTTACCAACT GCATGAGGTA TAATGCTAAA GACACGATTT TCCACCGAGC AGCTGTCCGG 
CTCAGAGACC TTGGAGGAGC GATATTGCGT CATGCACGGC GGCAGGCTGA AAACATCGGC 
TTTGACACTG ATGTGGGGAT TCACCTGCCC GAGTCACCCA AAACCGAGGA CTTTTACCGC 
TTTTCTTGGG AGGATGTGGA TAACATCCTC CTCCCAGAGA ACCGGGCTCA TCTCTCCTTG 
GAGGCCCAGC TGAAGGAGCT GCTGGAGAAG CTAGACATGG TGAGCACCAT GCGATCCAGC 
GGTGCCCGGA CACGACGCAT CCGGCTCCTG AGACGGGAGA TCAACTCCAT TCGGCAGAAA 
CTGGCCCAGC AGCAGAACAA AACCATGCCC AACGGGGAGC CTGTGCCGTG GGAAGAGGGG 
CTGGATAAAG CATCGAGGGA AGGGGATGAG GAAGGGGATA AAGATGATGC CAAGCCCCCG 
CACCCTCCAA CCCTGGAGCC CACAGGACCT GCGCCCTCTC TCTCAGAGCT GGACTCTCTG 
CAGGACCCTC CAATGCTCAA ACCCATCAGT GAAAGCAAGT CCTTGAATCA GCTGCAGAAG 
AGGGTGATGC CGGACAGGGA GCTCTTTGAC AAGAAGGCTC TGCAGCGGGA GAGCCAAGCC 
TTCCAGCGCC TGCTCAGCGA CAACGGCCTG AACGGACTGG CCTTGCCGCC TGCAGACACC 
CCAGCCAGCC CCCCGTTCAG TGGTGTGGGC CGACGGACAT CTGTCCTTTT TAAAAAAGCT 
AAGAACGGGG TGAAGCTGCA AAGAGGCCTG GACTGCTCCC TGGAGAATGG GGAGGACCAT 
GGGCAGAGTG GGCAGCTTTC ACCCTCGCGC CCTGATGGGG AACGACAAAC TCGGAAGCGG 
CCGCAGAGCA GGACCGGCAG CGAGAGCGAT GGAGAGAAGT CGCCCAGGCA GGCAGGACAG 
AGAGGAGTGA CTAACGGCTT TGTGAAGCAT GCAGAAAGCA GCTCTGACTC TGAGTGTAGC 
ACTGGCCGGG GCGGTGCGCT GGTGTTTGAA GCCTGCAGTG GTTTGATGCC TCCCAAGCGA 
AGTCGAGGCA AGCCAGCTCT TTCTCGGATG CCCTTCTTGG AAGGCGTGAA TGGAGACTCC 
GATTATGGCA GCTCAGGCAG GACTCTCCTG ATGTCCTTTG AGAGTCAGGC TGAGCTGGAG 
CCCTTGGAGC TTGTGTGGGC CAAGTGCCGT GGCTATCCCT CCTACCCTGC CTTGATAATC 
GATCCTAAGA TGCCGCGCGA GGGTTTGCTC CACAATGGAG TCCCCATCCC AGTCCCACCC 
ATGGATGTGT TGAAGCTGGG GGAGCAGAGG CAGACAGAGG CAGGAGAAAA GCTCTTCCTT 
GTCCTTTTCT TTGACAACAA GAGAACCTGG CAGTGGCTTC CGCGTGACAA AGTCTACCCC 
CTCGGCGTGG ACGACACAGT GGACAAGTTA AAAATGATGG AAGGCCGAAA AACCAGCATC 
CGCAAGTCTG TGCAAGTGGC ATATGACCGA GCCATGATCC ACATGAGCCG TGTGCGGGGA 
GATCATCCCT TCGTTACGTC CAGCTATCTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010300881.1
CDS	52..3564
Translation	MRKPRRRSRQNLEGRRSPSPYTLKCSPTRETLTYAQAQRIVEVDIDGRLHRISIYDPLKIITEDELTAQDITECNSNKENSEQPLFPSKSKKTPSKGKKKESCSKHAPGTSLHLPQPNFRVVDSFSQPHAPPLPAAYYRYIEKPPEDLDVEVEYDMDEEDLAWLEIVNEKRRDDGYGTVSADTFELLVDRLEKESYLESRNNGAQQSVIDEDAFCCVCMDDECHNSNVILFCDICNLAVHQECYGVPYIPEGQWLCRCCLQSPSRPVDCVLCPNKGGAFKQTSDGRWAHVVCAIWIPEVCFANTVFLEPIEGINNIPPARWKLTCYICKQKGMGAAIQCHKVNCYAAFHVTCAQRAGLFMKIEPMRETSINGTTFTVRKTAYCESHSPPGTVKKGSPAASGEGQEDTVKEEGEDKANCSPPKGSLKKNQVKLKQKIKKEPGAVTNGRSSMPMVTVAQIPSYRLNKICHGLSLQRKNQFMRRLHNYWLLKRQARNGVPLIRRLHSHLQSQRNAEQKEQDEKTSAVKEELKYWQKLRHDLERARLLIELIRKREKLKREQVKCQQAAMELQLTPFNVLLRTTLDLLQEKDAAQIFAEPVNLNEVPDYLEFISNPMDFSTMRRKLESHLYRTLDEFEEDFNLIVTNCMRYNAKDTIFHRAAVRLRDLGGAILRHARRQAENIGFDTDVGIHLPESPKTEDFYRFSWEDVDNILLPENRAHLSLEAQLKELLEKLDMVSTMRSSGARTRRIRLLRREINSIRQKLAQQQNKTMPNGEPVPWEEGLDKASREGDEEGDKDDAKPPHPPTLEPTGPAPSLSELDSLQDPPMLKPISESKSLNQLQKRVMPDRELFDKKALQRESQAFQRLLSDNGLNGLALPPADTPASPPFSGVGRRTSVLFKKAKNGVKLQRGLDCSLENGEDHGQSGQLSPSRPDGERQTRKRPQSRTGSESDGEKSPRQAGQRGVTNGFVKHAESSSDSECSTGRGGALVFEACSGLMPPKRSRGKPALSRMPFLEGVNGDSDYGSSGRTLLMSFESQAELEPLELVWAKCRGYPSYPALIIDPKMPREGLLHNGVPIPVPPMDVLKLGEQRQTEAGEKLFLVLFFDNKRTWQWLPRDKVYPLGVDDTVDKLKMMEGRKTSIRKSVQVAYDRAMIHMSRVRGDHPFVTSSYL

Target ORF information:

RefSeq Version	XM_010302579.1
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)
Definition	Balearica regulorum gibbericeps bromodomain and PHD finger containing, 3 (BRPF3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010302579.1

ORF Insert Sequence:

ATGAGGAAAC CTCGGAGGAG GTCCCGGCAG AACTTGGAGG GGAGACGTTC TCCCTCACCC 
TACACCCTCA AGTGCTCACC GACTCGGGAG ACACTGACGT ACGCTCAGGC CCAGCGGATC 
GTGGAGGTAG ACATCGATGG GCGTCTTCAT CGCATCAGCA TCTACGATCC CTTAAAAATC 
ATCACAGAGG ATGAGCTGAC AGCCCAGGAC ATCACAGAGT GCAACAGCAA CAAGGAAAAC 
AGCGAGCAGC CCCTTTTCCC TTCCAAATCT AAGAAAACAC CTTCCAAAGG CAAGAAGAAG 
GAGTCCTGCT CCAAACATGC ACCAGGGACA TCTTTACACT TACCCCAGCC CAACTTCCGT 
GTGGTGGACT CTTTCAGTCA GCCACATGCT CCTCCTCTCC CCGCTGCCTA CTACCGGTAC 
ATTGAAAAGC CTCCCGAGGA CCTTGATGTA GAGGTGGAGT ATGACATGGA TGAGGAGGAT 
CTGGCCTGGC TGGAAATAGT CAACGAGAAG AGAAGGGATG ATGGTTATGG GACAGTTAGT 
GCTGACACTT TTGAGCTGCT GGTGGACCGG TTGGAAAAGG AGTCGTACCT CGAGAGCCGG 
AACAACGGGG CTCAGCAGTC TGTCATCGAT GAGGATGCCT TCTGCTGCGT CTGCATGGAT 
GATGAGTGTC ACAACAGCAA CGTCATCCTG TTCTGTGATA TCTGCAATCT GGCTGTGCAC 
CAGGAGTGTT ACGGCGTGCC CTATATCCCT GAGGGGCAGT GGCTTTGCCG CTGCTGCTTA 
CAGTCCCCTT CTCGTCCTGT GGATTGTGTC CTTTGCCCGA ACAAAGGTGG AGCCTTCAAG 
CAGACCAGCG ATGGCCGCTG GGCTCATGTG GTTTGTGCCA TCTGGATCCC AGAGGTCTGC 
TTTGCAAACA CGGTGTTCCT GGAGCCCATT GAAGGGATAA ATAACATCCC CCCGGCTCGG 
TGGAAGCTCA CATGCTATAT CTGCAAGCAG AAGGGCATGG GAGCTGCTAT CCAATGCCAC 
AAGGTGAACT GTTATGCTGC CTTCCATGTC ACCTGTGCCC AGCGGGCTGG CCTCTTCATG 
AAGATCGAAC CCATGAGGGA GACCAGCATC AATGGCACAA CGTTCACCGT GCGCAAGACT 
GCCTATTGTG AGAGTCATTC CCCACCTGGA ACCGTGAAAA AAGGGTCTCC AGCTGCTAGT 
GGTGAGGGAC AGGAGGACAC TGTGAAAGAG GAGGGGGAGG ACAAAGCCAA TTGCAGCCCT 
CCCAAAGGGT CTCTGAAAAA GAACCAAGTG AAATTGAAGC AGAAGATCAA AAAGGAGCCT 
GGTGCTGTGA CCAATGGGCG CTCGTCTATG CCTATGGTGA CAGTCGCACA AATCCCCTCT 
TACAGGCTGA ACAAAATCTG CCACGGCCTT TCCCTCCAGA GGAAGAACCA GTTCATGAGG 
AGGCTCCACA ACTACTGGCT GCTGAAGCGA CAGGCGAGGA ACGGGGTGCC CCTGATCCGG 
CGCCTGCACT CACATCTCCA GTCTCAGAGG AACGCGGAGC AGAAGGAGCA GGATGAGAAG 
ACCAGTGCTG TGAAGGAAGA GCTGAAGTAC TGGCAAAAGC TGCGGCATGA CTTGGAGCGG 
GCACGGCTGC TCATCGAGCT GATCCGTAAG AGGGAAAAAC TCAAACGGGA GCAGGTCAAG 
TGTCAGCAGG CTGCAATGGA GCTACAGCTG ACCCCTTTCA ATGTACTTCT ACGCACAACT 
CTGGACCTGC TGCAGGAGAA GGATGCTGCC CAGATCTTTG CAGAGCCTGT TAACCTAAAT 
GAGGTTCCAG ATTACCTGGA ATTCATTTCC AACCCAATGG ATTTTTCCAC CATGAGGCGG 
AAGCTGGAGT CCCACCTGTA CCGAACATTG GATGAGTTTG AGGAAGACTT TAACCTTATA 
GTTACCAACT GCATGAGGTA TAATGCTAAA GACACGATTT TCCACCGAGC AGCTGTCCGG 
CTCAGAGACC TTGGAGGAGC GATATTGCGT CATGCACGGC GGCAGGCTGA AAACATCGGC 
TTTGACACTG ATGTGGGGAT TCACCTGCCC GAGTCACCCA AAACCGAGGA CTTTTACCGC 
TTTTCTTGGG AGGATGTGGA TAACATCCTC CTCCCAGAGA ACCGGGCTCA TCTCTCCTTG 
GAGGCCCAGC TGAAGGAGCT GCTGGAGAAG CTAGACATGG TGAGCACCAT GCGATCCAGC 
GGTGCCCGGA CACGACGCAT CCGGCTCCTG AGACGGGAGA TCAACTCCAT TCGGCAGAAA 
CTGGCCCAGC AGCAGAACAA AACCATGCCC AACGGGGAGC CTGTGCCGTG GGAAGAGGGG 
CTGGATAAAG CATCGAGGGA AGGGGATGAG GAAGGGGATA AAGATGATGC CAAGCCCCCG 
CACCCTCCAA CCCTGGAGCC CACAGGACCT GCGCCCTCTC TCTCAGAGCT GGACTCTCTG 
CAGGACCCTC CAATGCTCAA ACCCATCAGT GAAAGCAAGT CCTTGAATCA GCTGCAGAAG 
AGGGTGATGC CGGACAGGGA GCTCTTTGAC AAGAAGGCTC TGCAGCGGGA GAGCCAAGCC 
TTCCAGCGCC TGCTCAGCGA CAACGGCCTG AACGGACTGG CCTTGCCGCC TGCAGACACC 
CCAGCCAGCC CCCCGTTCAG TGGTGTGGGC CGACGGACAT CTGTCCTTTT TAAAAAAGCT 
AAGAACGGGG TGAAGCTGCA AAGAGGCCTG GACTGCTCCC TGGAGAATGG GGAGGACCAT 
GGGCAGAGTG GGCAGCTTTC ACCCTCGCGC CCTGATGGGG AACGACAAAC TCGGAAGCGG 
CCGCAGAGCA GGACCGGCAG CGAGAGCGAT GGAGAGAAGT CGCCCAGGCA GGCAGGACAG 
AGAGGAGTGA CTAACGGCTT TGTGAAGCAT GCAGAAAGCA GCTCTGACTC TGAGTGTAGC 
ACTGGCCGGG GCGGTGCGCT GGTGTTTGAA GCCTGCAGTG GTTTGATGCC TCCCAAGCGA 
AGTCGAGGCA AGCCAGCTCT TTCTCGGATG CCCTTCTTGG AAGGCGTGAA TGGAGACTCC 
GATTATGGCA GCTCAGGCAG GACTCTCCTG ATGTCCTTTG AGAGTCAGGC TGAGCTGGAG 
CCCTTGGAGC TTGTGTGGGC CAAGTGCCGT GGCTATCCCT CCTACCCTGC CTTGATAATC 
GATCCTAAGA TGCCGCGCGA GGGTTTGCTC CACAATGGAG TCCCCATCCC AGTCCCACCC 
ATGGATGTGT TGAAGCTGGG GGAGCAGAGG CAGACAGAGG CAGGAGAAAA GCTCTTCCTT 
GTCCTTTTCT TTGACAACAA GAGAACCTGG CAGTGGCTTC CGCGTGACAA AGTCTACCCC 
CTCGGCGTGG ACGACACAGT GGACAAGTTA AAAATGATGG AAGGCCGAAA AACCAGCATC 
CGCAAGTCTG TGCAAGTGGC ATATGACCGA GCCATGATCC ACATGAGCCG TGTGCGGGGA 
GATCATCCCT TCGTTACGTC CAGCTATCTG TAA