TARS cDNA ORF clone, Balearica regulorum gibbericeps(East African grey crowned-crane)

Gene
Clones
gRNAs

Summary

Gene Symbol	TARS
Entrez Gene ID	104635489
Full Name	threonyl-tRNA synthetase
Gene Type	protein-coding
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010302325.1	XP_010300627.1	threonine--tRNA ligase, cytoplasmic

Pathways from BioSystems
Gene Ontology

The following TARS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TARS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa186590	XM_010302325.1 Latest version!	Balearica regulorum gibbericeps threonyl-tRNA synthetase (TARS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TARS ( XM_010302325.1 ) cDNA ORF clone, Balearica regulorum gibbericeps(East African grey crowned-crane) -> XP_010300627.1 Balearica regulorum gibbericeps threonyl-tRNA synthetase (TARS), mRNA.

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CloneID	OBa186590
Clone ID Related Accession (Same CDS sequence)	XM_010302325.1
Accession Version	XM_010302325.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-17
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)
Product	threonine--tRNA ligase, cytoplasmic
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010755167.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Balearica regulorum gibbericeps Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAAG AGGCCGGCGA TGGAGGGCGG ACGGAGCTGA ATCCCTGGCC TGCATTTATC 
AATGAGCGTT TAGAGATGTA CAATAAACTT AAAGCTGAAC ATGATGCGCT CCTTGCAGAA 
AGAGCTGCAA ATGACAGTAA ACCCATTAAA GTTACATTAC CCGATGGCAA GCAGGTTGAT 
GCTGAATCCT GGAAGACTAC TCCTTATCAA ATCGCTTGTG GAATTAGTCA GGGCTTAGCT 
GACAACACTG TTATTGCTAA AGTGAACAAG ATGGTTTGGG ATCTAGACCG TCCTTTGGAG 
GAGGATTGTA CCCTGGAGCT GCTTAAGTTT GAAGATGAAG AGGCTCAAGC AGTATACTGG 
CACTCAAGTG CTCACATAAT GGGTGAAGCT ATGGAGCGAA TCTATGGTGG CTGTTTGTGC 
TATGGCCCAC CAATAGAAAA TGGATTTTAT TATGACATGT TCCTTGAGGA AGGGGGTGTA 
TCAAGTAATG ACTTCTCTGC TTTGGAAACA TTATGCAAAA AGATAATGAA GGAAAAACAA 
GTCTTTGAAA GACTGGAAGT TAAGAAGGAA ACGCTACTTG AAATGTTTAA GTATAATAAA 
TTCAAGTGTC GCATCCTGAA TGAGAAGGTC AATACTCCAA CTACAACAGT ATACAGGTGT 
GGTCCCTTGA TAGATTTATG CAGAGGACCT CATGTCAGAC ATACTGGCAA GATAAAGACC 
ATAAAAATTC ATAAGAATTC TTCTACCTAT TGGGAAGGCA AGGCTGATAT GGAATCCCTC 
CAGCGGATCT ATGGAATTTC ATTCCCAGAT GCAAAAATGC TGAAGGAATG GGAGAAATTC 
CAGGAGGAGG CTAAAAGCAG AGATCACAGA AAAATTGGGC GGGACCAAGA ACTATTTTTC 
TTTCATGAGC TCAGCCCTGG TAGCTGTTTT TTCCTGCCAA AAGGAGCTTA CATTTATAAC 
ACATTAATTG AATTCATACG GAGTGAGTAT CGAAAACGTG GATTCCAGGA GGTTGTCACT 
CCAAATGTTT TCAACAGCAA ACTATGGATG ACTTCAGGGC ACTGGCAGCA TTACAGCGAC 
AACATGTTTT CCTTTGAAGT GGAGAAAGAA ATCTTTGCTC TGAAGCCTAT GAACTGTCCA 
GGACACTGCC TTATGTTTGA TCATCGTCCA AGATCATGGC GTGAGCTGCC TTTACGGTTG 
GCTGATTTTG GTGTTCTGCA TCGCAATGAA CTGTCAGGAG CTCTTACAGG ACTCACTCGA 
GTACGCCGAT TCCAGCAGGA TGATGCTCAC ATATTCTGTG CTATGGAGCA GATTGAAGAG 
GAGATAAAGA GTTGTCTGCA GTTCTTGCGT TCTGTGTATG ATGTCTTTGG ATTTTCCTTT 
AAATTGAATC TCTCCACTCG TCCTGAAAAA TATCTGGGAA ATATTGAAGT GTGGAATCAA 
GCCGAAAAGC AACTAGAAAA CAGCCTCAAT GACTTTGGTG AGAAGTGGGA GTTAAACCCT 
GGTGATGGAG CTTTCTATGG ACCTAAGATT GACATTCAGA TTAAAGATGC CATTGGCCGC 
TACCACCAAT GTGCTACAAT CCAGCTTGAC TTTCAGCTGC CAGTCAGATT TAACCTCACC 
TTTGTCAGCA ATGATGGTAA TGACAAGACA AGACCAGTTA TCATTCACCG GGCTATCCTG 
GGATCTGTGG AGAGAATGAT AGCCATTCTA ACTGAAAACT ATGGAGGCAA ATGGCCACTC 
TGGCTGTCCC CACAGCAGGT AATGGTGGTA CCGGTGGGAC CAACGTGTGA TGAGTATGCT 
CAAAAGGTGA TGCAGCACTT CCACGATGCT GGATTAATGG CAGATGTTGA TGTAGATCCT 
GGGTGCACAC TGAACAAGAA GATCAGAAAT GCTCAGCTTG CACAGTATAA CTTTATTCTG 
GTTGTTGGTG AAAAGGAGAA GGCAAGTGGA ACGGTTAACA TCCGCACCCG AGATAACAAG 
GTGCACGGTG AGCGTACAAT TGCAGACACT GTGAAGAGAC TGCTGGAACT GAAATGCTCT 
CGCTCCAGAC AAGCTGAAGA GGAGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010300627.1
CDS	43..2112
Translation	MKEEAGDGGRTELNPWPAFINERLEMYNKLKAEHDALLAERAANDSKPIKVTLPDGKQVDAESWKTTPYQIACGISQGLADNTVIAKVNKMVWDLDRPLEEDCTLELLKFEDEEAQAVYWHSSAHIMGEAMERIYGGCLCYGPPIENGFYYDMFLEEGGVSSNDFSALETLCKKIMKEKQVFERLEVKKETLLEMFKYNKFKCRILNEKVNTPTTTVYRCGPLIDLCRGPHVRHTGKIKTIKIHKNSSTYWEGKADMESLQRIYGISFPDAKMLKEWEKFQEEAKSRDHRKIGRDQELFFFHELSPGSCFFLPKGAYIYNTLIEFIRSEYRKRGFQEVVTPNVFNSKLWMTSGHWQHYSDNMFSFEVEKEIFALKPMNCPGHCLMFDHRPRSWRELPLRLADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCAMEQIEEEIKSCLQFLRSVYDVFGFSFKLNLSTRPEKYLGNIEVWNQAEKQLENSLNDFGEKWELNPGDGAFYGPKIDIQIKDAIGRYHQCATIQLDFQLPVRFNLTFVSNDGNDKTRPVIIHRAILGSVERMIAILTENYGGKWPLWLSPQQVMVVPVGPTCDEYAQKVMQHFHDAGLMADVDVDPGCTLNKKIRNAQLAQYNFILVVGEKEKASGTVNIRTRDNKVHGERTIADTVKRLLELKCSRSRQAEEEF

Target ORF information:

RefSeq Version	XM_010302325.1
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)
Definition	Balearica regulorum gibbericeps threonyl-tRNA synthetase (TARS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010302325.1

ORF Insert Sequence:

ATGAAGGAAG AGGCCGGCGA TGGAGGGCGG ACGGAGCTGA ATCCCTGGCC TGCATTTATC 
AATGAGCGTT TAGAGATGTA CAATAAACTT AAAGCTGAAC ATGATGCGCT CCTTGCAGAA 
AGAGCTGCAA ATGACAGTAA ACCCATTAAA GTTACATTAC CCGATGGCAA GCAGGTTGAT 
GCTGAATCCT GGAAGACTAC TCCTTATCAA ATCGCTTGTG GAATTAGTCA GGGCTTAGCT 
GACAACACTG TTATTGCTAA AGTGAACAAG ATGGTTTGGG ATCTAGACCG TCCTTTGGAG 
GAGGATTGTA CCCTGGAGCT GCTTAAGTTT GAAGATGAAG AGGCTCAAGC AGTATACTGG 
CACTCAAGTG CTCACATAAT GGGTGAAGCT ATGGAGCGAA TCTATGGTGG CTGTTTGTGC 
TATGGCCCAC CAATAGAAAA TGGATTTTAT TATGACATGT TCCTTGAGGA AGGGGGTGTA 
TCAAGTAATG ACTTCTCTGC TTTGGAAACA TTATGCAAAA AGATAATGAA GGAAAAACAA 
GTCTTTGAAA GACTGGAAGT TAAGAAGGAA ACGCTACTTG AAATGTTTAA GTATAATAAA 
TTCAAGTGTC GCATCCTGAA TGAGAAGGTC AATACTCCAA CTACAACAGT ATACAGGTGT 
GGTCCCTTGA TAGATTTATG CAGAGGACCT CATGTCAGAC ATACTGGCAA GATAAAGACC 
ATAAAAATTC ATAAGAATTC TTCTACCTAT TGGGAAGGCA AGGCTGATAT GGAATCCCTC 
CAGCGGATCT ATGGAATTTC ATTCCCAGAT GCAAAAATGC TGAAGGAATG GGAGAAATTC 
CAGGAGGAGG CTAAAAGCAG AGATCACAGA AAAATTGGGC GGGACCAAGA ACTATTTTTC 
TTTCATGAGC TCAGCCCTGG TAGCTGTTTT TTCCTGCCAA AAGGAGCTTA CATTTATAAC 
ACATTAATTG AATTCATACG GAGTGAGTAT CGAAAACGTG GATTCCAGGA GGTTGTCACT 
CCAAATGTTT TCAACAGCAA ACTATGGATG ACTTCAGGGC ACTGGCAGCA TTACAGCGAC 
AACATGTTTT CCTTTGAAGT GGAGAAAGAA ATCTTTGCTC TGAAGCCTAT GAACTGTCCA 
GGACACTGCC TTATGTTTGA TCATCGTCCA AGATCATGGC GTGAGCTGCC TTTACGGTTG 
GCTGATTTTG GTGTTCTGCA TCGCAATGAA CTGTCAGGAG CTCTTACAGG ACTCACTCGA 
GTACGCCGAT TCCAGCAGGA TGATGCTCAC ATATTCTGTG CTATGGAGCA GATTGAAGAG 
GAGATAAAGA GTTGTCTGCA GTTCTTGCGT TCTGTGTATG ATGTCTTTGG ATTTTCCTTT 
AAATTGAATC TCTCCACTCG TCCTGAAAAA TATCTGGGAA ATATTGAAGT GTGGAATCAA 
GCCGAAAAGC AACTAGAAAA CAGCCTCAAT GACTTTGGTG AGAAGTGGGA GTTAAACCCT 
GGTGATGGAG CTTTCTATGG ACCTAAGATT GACATTCAGA TTAAAGATGC CATTGGCCGC 
TACCACCAAT GTGCTACAAT CCAGCTTGAC TTTCAGCTGC CAGTCAGATT TAACCTCACC 
TTTGTCAGCA ATGATGGTAA TGACAAGACA AGACCAGTTA TCATTCACCG GGCTATCCTG 
GGATCTGTGG AGAGAATGAT AGCCATTCTA ACTGAAAACT ATGGAGGCAA ATGGCCACTC 
TGGCTGTCCC CACAGCAGGT AATGGTGGTA CCGGTGGGAC CAACGTGTGA TGAGTATGCT 
CAAAAGGTGA TGCAGCACTT CCACGATGCT GGATTAATGG CAGATGTTGA TGTAGATCCT 
GGGTGCACAC TGAACAAGAA GATCAGAAAT GCTCAGCTTG CACAGTATAA CTTTATTCTG 
GTTGTTGGTG AAAAGGAGAA GGCAAGTGGA ACGGTTAACA TCCGCACCCG AGATAACAAG 
GTGCACGGTG AGCGTACAAT TGCAGACACT GTGAAGAGAC TGCTGGAACT GAAATGCTCT 
CGCTCCAGAC AAGCTGAAGA GGAGTTTTAA