DDX58 cDNA ORF clone, Balaenoptera acutorostrata scammoni

Gene
Clones
gRNAs

Summary

Gene Symbol	DDX58
Entrez Gene ID	103020032
Full Name	DExD/H-box helicase 58
Gene Type	protein-coding
Organism	Balaenoptera acutorostrata scammoni

mRNA and Protein(s)

mRNA	Protein	Name
XM_007176846.1	XP_007176908.1	LOW QUALITY PROTEIN: probable ATP-dependent RNA helicase DDX58
XM_028165158.1	XP_028020959.1	LOW QUALITY PROTEIN: probable ATP-dependent RNA helicase DDX58

Pathways from BioSystems
Gene Ontology

The following DDX58 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDX58 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa157304	XM_007176846.1 Latest version!	Balaenoptera acutorostrata scammoni DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 (DDX58), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBa241851	XM_028165158.1 Latest version!	Balaenoptera acutorostrata scammoni DExD/H-box helicase 58 (DDX58), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DDX58 ( XM_007176846.1 ) cDNA ORF clone, Balaenoptera acutorostrata scammoni -> XP_007176908.1 Balaenoptera acutorostrata scammoni DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 (DDX58), mRNA.

Price & Availability↑

CloneID	OBa157304
Clone ID Related Accession (Same CDS sequence)	XM_007176846.1
Accession Version	XM_007176846.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-16
Organism	Balaenoptera acutorostrata scammoni
Product	LOW QUALITY PROTEIN: probable ATP-dependent RNA helicase DDX58
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006726353.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Balaenoptera acutorostrata scammoni Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGCCG AGCAGCTGCA GAGTCTGCAT GCCTTCCGGG ACCATGTCAG GAACTTCGTG 
GACTCTACCT ACATCCTGAG CTACGTGACG CCCTGGTTTA GGGACGATGA GGTGCAGCAT 
ATTCAGGCTG AGAAAAACAA CAAGGGCCCA ATGGAGGCTG CCTCACTTTT TCTCCAGTTC 
CTGTTGGAGC TCCAGGAGGA AAGCTGGTTC TGCAGCTTTT TGGATGCCCT TGACCAAGCA 
GGTTATTCTG GACTTGATGA TGCCATTGAA AGTTGGGATT TCCAAAAAAT TGAAAGGTTG 
GAGGAGTATA GATTACTTTT AAAGCATTTA CAACCAGAAT TTAAATCCAC GATCAATCCA 
AAAGGTATCC TTCCTGAAAT ATCTGAACGT TTAATTAATC AGGAATGTGA AGAAATTATT 
CAGATTTGTT CCATCAAGGG GCTGATGGCA AGTGCAGAGA AAATGGTTGA ATGCCTTCTC 
AGATCAGACA AGGAGAACTG GCCCAAAACT TTGAAACTTG CATTGGAGAA AGAAGAGAGC 
AAGTTCAGTG AACTGTGGAT GGTAGAGAAA GGTGCAAAAG ATGTCAAAAT GAAAGATCTT 
GAGGATGATG AAATGAAAAC TTTGGATGTA CAGATTTTCT GCAGGGAAGA ACCAGAAAAC 
CAGAATCTTA GTCAGAATTC ATGCCCTTCT TCAGAAGTGC CTCATACTTG CAGCCCATTG 
AAGCCAAGAA ATTACCAACT AGAGCTTGCT TTACCTGCTC AGAAAGGAAA AAACACAATA 
ATATGTGCTC CTACTGGTTG TGGAAAAACC TTTGTTTCAC TTCTTATATG TGAACATCAT 
CTTAAAAAAT TTCCACAAGG ACAAAAGGGG AAAGTTGTCT TTTTTGCTAT TCAACTCCCA 
GTGTATGAGC AGCAGAAATC TGTGTTCTCA AAGTATTTTG AAAGACTTGG GTACAAAGTT 
GGAGGCATTT CTGGAGTAAC ATCTGACAGT ATCTCAGTGG AACAGACTGT TGAGAACAAT 
GACATCATCA TTTTAACTCC ACAGATTCTT GTGAACAGCC TTAAAAATGG GACGATTCCA 
TCACTCTCTG TCTTTACTTT GATGATATTT GATGAATGCC ACAACACTAG TAAACATCAT 
CCTTACAATA TGATCATGTT TAATTATCTA GATCAGAAAC TTGGAGGATC TTCAGACTCA 
TTGCCCCAGG TCATGGGGCT GACTGCCTCG GTTGGCACTG GGGATGCCAA AAATACAGCT 
GAAGCCACAG AATATTTCTG CAAATTGTGT GCTTCTCTTG ACACATCAGT GGTAGCAACA 
GTCAAAGACA ACTTGGAGGA ACTGGAGGAG ATTGTTTATA AGCCCCAGAA GTTTTTCAGG 
AAAGTGGAAT CACGGACCAC CGACAGATTT AAATGCAACA TATCTCAGTT GATGGTAGAG 
NNACAGAGTC TGGTAAAGAG TATCTTTGAA GAACTTGGTA CCATAACTCT CGAAAACTTA 
TCTCAAATTC AAAATAGGAA TTTTGGAACA CAGAGATATG AACAGTGGAT TATCGCAGCT 
CAGAAAGTGC GCATGGTGTT TCAGATGCCA NAAGATGAAG AGAGAAGGAT TTGTAAAGCA 
CAGTTTTTCT ACACGTCGCG TTTCAACAGA TACAATGATG CCCTCATTAT CAATGAGCGT 
GCTCGAATGA AAAATGCTCT GGATTACTTA AAAGACTTCT TCAGAAATGT CCGAGCAGCA 
GGATTTGATG AGATTGAGCA AGCTCTCACT CAGAGATTTG AAGAAAAGCT GCAGGAACTA 
GAAAGTATTT CCATGGATCC CAGCAATGAG AACCCTAAAC TCAAAGATCT CTGCTTCATC 
TTGCAAGAAG AGTACCACTT AAGCCCAGAG ACCAGAATCA TCCTCTTTGG GAAAAACAAA 
GCACTCGTGG ATGCTTTAAA GAAATGGATT GAAGAAAATC CTAAGCTCAG CTTTCTAAAA 
CCTGGCATAT TGACTGGACG TGGCAGAACA AATCAGAAAA TAGGAATGAC CCTCCCAGTA 
CAGAAGTGTG TATTGGATGC ATTCAGAACC GACGGAGATA ACAAGATTCT GATTGCCACC 
TCGGTTGCTG ATGAAGGTAT TGACATTGCT CAGTGCAATC TGGTCCTCCT ATATGAGTAT 
GTGGGCAATG TCATCAAAAT GATCCAAACC AGAGGCAGAG GAAGAGCAAG AGGGAGCAAG 
TGCTTCCTTC TGACTAGTAA TATGAATGAC TCCATTTTAA GCCTGCAGAC GTGAGATGAA 
GCAGTATTTA AAGAAAAGGT TTGCCAGATA CAGATTCATG AAAAATTCAT CAGGGATAAT 
CAAGGAAAAG CAAAACCTGG GCTTGATAAG GAAAATAAAA AACTGCTCTG CAGAAAGTGC 
AAAACCTTTG CATGTTATAC AGTTGATATA AGAGTGATGG AGGAATGCCA TTTCATTGTG 
GTTGGAGTTG CTTTCAGGGA GCACTTTGTG AGTAAGTTAC ACCCCAGACC AAAGAGCTTT 
GGGAGTTTTG AGAAGAGAGC AAAGATCTTC TGTGCCAGGG AGGACTGCAG CTATGACTGG 
GGAATCCATG GGAAGTATAA GGTATTTGAG ATTCCAGTTA TAAAGATTCA AAGTTTTGTG 
GTGGAAGATG TTGCAACTGG AGCTCAGACA CTGTATGCAA AGTGGAAGGA CTTTCATTTT 
GAGACCATCC CATTTGATGC TGCAGAAATG TCCACTTGAG CTCAGGACCT CAGTCTGCAG 
GGAATGGGTG ACCTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007176908.1
CDS	155..2935
Translation	MTAEQLQSLHAFRDHVRNFVDSTYILSYVTPWFRDDEVQHIQAEKNNKGPMEAASLFLQFLLELQEESWFCSFLDALDQAGYSGLDDAIESWDFQKIERLEEYRLLLKHLQPEFKSTINPKGILPEISERLINQECEEIIQICSIKGLMASAEKMVECLLRSDKENWPKTLKLALEKEESKFSELWMVEKGAKDVKMKDLEDDEMKTLDVQIFCREEPENQNLSQNSCPSSEVPHTCSPLKPRNYQLELALPAQKGKNTIICAPTGCGKTFVSLLICEHHLKKFPQGQKGKVVFFAIQLPVYEQQKSVFSKYFERLGYKVGGISGVTSDSISVEQTVENNDIIILTPQILVNSLKNGTIPSLSVFTLMIFDECHNTSKHHPYNMIMFNYLDQKLGGSSDSLPQVMGLTASVGTGDAKNTAEATEYFCKLCASLDTSVVATVKDNLEELEEIVYKPQKFFRKVESRTTDRFKCNISQLMVEnullQSLVKSIFEELGTITLENLSQIQNRNFGTQRYEQWIIAAQKVRMVFQMPnullDEERRICKAQFFYTSRFNRYNDALIINERARMKNALDYLKDFFRNVRAAGFDEIEQALTQRFEEKLQELESISMDPSNENPKLKDLCFILQEEYHLSPETRIILFGKNKALVDALKKWIEENPKLSFLKPGILTGRGRTNQKIGMTLPVQKCVLDAFRTDGDNKILIATSVADEGIDIAQCNLVLLYEYVGNVIKMIQTRGRGRARGSKCFLLTSNMNDSILSLQTDEAVFKEKVCQIQIHEKFIRDNQGKAKPGLDKENKKLLCRKCKTFACYTVDIRVMEECHFIVVGVAFREHFVSKLHPRPKSFGSFEKRAKIFCAREDCSYDWGIHGKYKVFEIPVIKIQSFVVEDVATGAQTLYAKWKDFHFETIPFDAAEMSTAQDLSLQGMGDLE

Target ORF information:

RefSeq Version	XM_007176846.1
Organism	Balaenoptera acutorostrata scammoni
Definition	Balaenoptera acutorostrata scammoni DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 (DDX58), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007176846.1

ORF Insert Sequence:

ATGACCGCCG AGCAGCTGCA GAGTCTGCAT GCCTTCCGGG ACCATGTCAG GAACTTCGTG 
GACTCTACCT ACATCCTGAG CTACGTGACG CCCTGGTTTA GGGACGATGA GGTGCAGCAT 
ATTCAGGCTG AGAAAAACAA CAAGGGCCCA ATGGAGGCTG CCTCACTTTT TCTCCAGTTC 
CTGTTGGAGC TCCAGGAGGA AAGCTGGTTC TGCAGCTTTT TGGATGCCCT TGACCAAGCA 
GGTTATTCTG GACTTGATGA TGCCATTGAA AGTTGGGATT TCCAAAAAAT TGAAAGGTTG 
GAGGAGTATA GATTACTTTT AAAGCATTTA CAACCAGAAT TTAAATCCAC GATCAATCCA 
AAAGGTATCC TTCCTGAAAT ATCTGAACGT TTAATTAATC AGGAATGTGA AGAAATTATT 
CAGATTTGTT CCATCAAGGG GCTGATGGCA AGTGCAGAGA AAATGGTTGA ATGCCTTCTC 
AGATCAGACA AGGAGAACTG GCCCAAAACT TTGAAACTTG CATTGGAGAA AGAAGAGAGC 
AAGTTCAGTG AACTGTGGAT GGTAGAGAAA GGTGCAAAAG ATGTCAAAAT GAAAGATCTT 
GAGGATGATG AAATGAAAAC TTTGGATGTA CAGATTTTCT GCAGGGAAGA ACCAGAAAAC 
CAGAATCTTA GTCAGAATTC ATGCCCTTCT TCAGAAGTGC CTCATACTTG CAGCCCATTG 
AAGCCAAGAA ATTACCAACT AGAGCTTGCT TTACCTGCTC AGAAAGGAAA AAACACAATA 
ATATGTGCTC CTACTGGTTG TGGAAAAACC TTTGTTTCAC TTCTTATATG TGAACATCAT 
CTTAAAAAAT TTCCACAAGG ACAAAAGGGG AAAGTTGTCT TTTTTGCTAT TCAACTCCCA 
GTGTATGAGC AGCAGAAATC TGTGTTCTCA AAGTATTTTG AAAGACTTGG GTACAAAGTT 
GGAGGCATTT CTGGAGTAAC ATCTGACAGT ATCTCAGTGG AACAGACTGT TGAGAACAAT 
GACATCATCA TTTTAACTCC ACAGATTCTT GTGAACAGCC TTAAAAATGG GACGATTCCA 
TCACTCTCTG TCTTTACTTT GATGATATTT GATGAATGCC ACAACACTAG TAAACATCAT 
CCTTACAATA TGATCATGTT TAATTATCTA GATCAGAAAC TTGGAGGATC TTCAGACTCA 
TTGCCCCAGG TCATGGGGCT GACTGCCTCG GTTGGCACTG GGGATGCCAA AAATACAGCT 
GAAGCCACAG AATATTTCTG CAAATTGTGT GCTTCTCTTG ACACATCAGT GGTAGCAACA 
GTCAAAGACA ACTTGGAGGA ACTGGAGGAG ATTGTTTATA AGCCCCAGAA GTTTTTCAGG 
AAAGTGGAAT CACGGACCAC CGACAGATTT AAATGCAACA TATCTCAGTT GATGGTAGAG 
NNACAGAGTC TGGTAAAGAG TATCTTTGAA GAACTTGGTA CCATAACTCT CGAAAACTTA 
TCTCAAATTC AAAATAGGAA TTTTGGAACA CAGAGATATG AACAGTGGAT TATCGCAGCT 
CAGAAAGTGC GCATGGTGTT TCAGATGCCA NAAGATGAAG AGAGAAGGAT TTGTAAAGCA 
CAGTTTTTCT ACACGTCGCG TTTCAACAGA TACAATGATG CCCTCATTAT CAATGAGCGT 
GCTCGAATGA AAAATGCTCT GGATTACTTA AAAGACTTCT TCAGAAATGT CCGAGCAGCA 
GGATTTGATG AGATTGAGCA AGCTCTCACT CAGAGATTTG AAGAAAAGCT GCAGGAACTA 
GAAAGTATTT CCATGGATCC CAGCAATGAG AACCCTAAAC TCAAAGATCT CTGCTTCATC 
TTGCAAGAAG AGTACCACTT AAGCCCAGAG ACCAGAATCA TCCTCTTTGG GAAAAACAAA 
GCACTCGTGG ATGCTTTAAA GAAATGGATT GAAGAAAATC CTAAGCTCAG CTTTCTAAAA 
CCTGGCATAT TGACTGGACG TGGCAGAACA AATCAGAAAA TAGGAATGAC CCTCCCAGTA 
CAGAAGTGTG TATTGGATGC ATTCAGAACC GACGGAGATA ACAAGATTCT GATTGCCACC 
TCGGTTGCTG ATGAAGGTAT TGACATTGCT CAGTGCAATC TGGTCCTCCT ATATGAGTAT 
GTGGGCAATG TCATCAAAAT GATCCAAACC AGAGGCAGAG GAAGAGCAAG AGGGAGCAAG 
TGCTTCCTTC TGACTAGTAA TATGAATGAC TCCATTTTAA GCCTGCAGAC GTGAGATGAA 
GCAGTATTTA AAGAAAAGGT TTGCCAGATA CAGATTCATG AAAAATTCAT CAGGGATAAT 
CAAGGAAAAG CAAAACCTGG GCTTGATAAG GAAAATAAAA AACTGCTCTG CAGAAAGTGC 
AAAACCTTTG CATGTTATAC AGTTGATATA AGAGTGATGG AGGAATGCCA TTTCATTGTG 
GTTGGAGTTG CTTTCAGGGA GCACTTTGTG AGTAAGTTAC ACCCCAGACC AAAGAGCTTT 
GGGAGTTTTG AGAAGAGAGC AAAGATCTTC TGTGCCAGGG AGGACTGCAG CTATGACTGG 
GGAATCCATG GGAAGTATAA GGTATTTGAG ATTCCAGTTA TAAAGATTCA AAGTTTTGTG 
GTGGAAGATG TTGCAACTGG AGCTCAGACA CTGTATGCAA AGTGGAAGGA CTTTCATTTT 
GAGACCATCC CATTTGATGC TGCAGAAATG TCCACTTGAG CTCAGGACCT CAGTCTGCAG 
GGAATGGGTG ACCTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX58 ( XM_028165158.1 ) cDNA ORF clone, Balaenoptera acutorostrata scammoni -> XP_028020959.1 Balaenoptera acutorostrata scammoni DExD/H-box helicase 58 (DDX58), mRNA.

Price & Availability↑

CloneID	OBa241851
Clone ID Related Accession (Same CDS sequence)	XM_028165158.1
Accession Version	XM_028165158.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-11
Organism	Balaenoptera acutorostrata scammoni
Product	LOW QUALITY PROTEIN: probable ATP-dependent RNA helicase DDX58
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006726353.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Balaenoptera acutorostrata scammoni Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 2 indels internal stop codons :: corrected 2 genomic stop codons ##RefSeq-Attributes-END##

ATGACCGCCG AGCAGCTGCA GAGTCTGCAT GCCTTCCGGG ACCATGTCAG GAACTTCGTG 
GACTCTACCT ACATCCTGAG CTACGTGACG CCCTGGTTTA GGGACGATGA GGTGCAGCAT 
ATTCAGGCTG AGAAAAACAA CAAGGGCCCA ATGGAGGCTG CCTCACTTTT TCTCCAGTTC 
CTGTTGGAGC TCCAGGAGGA AAGCTGGTTC TGCAGCTTTT TGGATGCCCT TGACCAAGCA 
GGTTATTCTG GACTTGATGA TGCCATTGAA AGTTGGGATT TCCAAAAAAT TGAAAGGTTG 
GAGGAGTATA GATTACTTTT AAAGCATTTA CAACCAGAAT TTAAATCCAC GATCAATCCA 
AAAGGTATCC TTCCTGAAAT ATCTGAACGT TTAATTAATC AGGAATGTGA AGAAATTATT 
CAGATTTGTT CCATCAAGGG GCTGATGGCA AGTGCAGAGA AAATGGTTGA ATGCCTTCTC 
AGATCAGACA AGGAGAACTG GCCCAAAACT TTGAAACTTG CATTGGAGAA AGAAGAGAGC 
AAGTTCAGTG AACTGTGGAT GGTAGAGAAA GGTGCAAAAG ATGTCAAAAT GAAAGATCTT 
GAGGATGATG AAATGAAAAC TTTGGATGTA CAGATTTTCT GCAGGGAAGA ACCAGAAAAC 
CAGAATCTTA GTCAGAATTC ATGCCCTTCT TCAGAAGTGC CTCATACTTG CAGCCCATTG 
AAGCCAAGAA ATTACCAACT AGAGCTTGCT TTACCTGCTC AGAAAGGAAA AAACACAATA 
ATATGTGCTC CTACTGGTTG TGGAAAAACC TTTGTTTCAC TTCTTATATG TGAACATCAT 
CTTAAAAAAT TTCCACAAGG ACAAAAGGGG AAAGTTGTCT TTTTTGCTAT TCAACTCCCA 
GTGTATGAGC AGCAGAAATC TGTGTTCTCA AAGTATTTTG AAAGACTTGG GTACAAAGTT 
GGAGGCATTT CTGGAGTAAC ATCTGACAGT ATCTCAGTGG AACAGACTGT TGAGAACAAT 
GACATCATCA TTTTAACTCC ACAGATTCTT GTGAACAGCC TTAAAAATGG GACGATTCCA 
TCACTCTCTG TCTTTACTTT GATGATATTT GATGAATGCC ACAACACTAG TAAACATCAT 
CCTTACAATA TGATCATGTT TAATTATCTA GATCAGAAAC TTGGAGGATC TTCAGACTCA 
TTGCCCCAGG TCATGGGGCT GACTGCCTCG GTTGGCACTG GGGATGCCAA AAATACAGCT 
GAAGCCACAG AATATTTCTG CAAATTGTGT GCTTCTCTTG ACACATCAGT GGTAGCAACA 
GTCAAAGACA ACTTGGAGGA ACTGGAGGAG ATTGTTTATA AGCCCCAGAA GTTTTTCAGG 
AAAGTGGAAT CACGGACCAC CGACAGATTT AAATGCAACA TATCTCAGTT GATGGTAGAG 
ACAGAATGAC TGGCCAGATG GCACAATGAA AAACTTTTTT TTTTTTCAGA AAACTTATCT 
CAAATTCAAA ATAGGAATTT TGGAACACAG AGATATGAAC AGTGGATTAT CGCAGCTCAG 
AAAGTGCGCA TGGTGTTTCA GATGCCANAA GATGAAGAGA GAAGGATTTG TAAAGCACAG 
TTTTTCTACA CGTCGCGTTT CAACAGATAC AATGATGCCC TCATTATCAA TGAGCGTGCT 
CGAATGAAAA ATGCTCTGGA TTACTTAAAA GACTTCTTCA GAAATGTCCG AGCAGCAGGA 
TTTGATGAGA TTGAGCAAGC TCTCACTCAG AGATTTGAAG AAAAGCTGCA GGAACTAGAA 
AGTATTTCCA TGGATCCCAG CAATGAGAAC CCTAAACTCA AAGATCTCTG CTTCATCTTG 
CAAGAAGAGT ACCACTTAAG CCCAGAGACC AGAATCATCC TCTTTGGGAA AAACAAAGCA 
CTCGTGGATG CTTTAAAGAA ATGGATTGAA GAAAATCCTA AGCTCAGCTT TCTAAAACCT 
GGCATATTGA CTGGACGTGG CAGAACAAAT CAGAAAATAG GAATGACCCT CCCAGTACAG 
AAGTGTGTAT TGGATGCATT CAGAACCGAC GGAGATAACA AGATTCTGAT TGCCACCTCG 
GTTGCTGATG AAGGTATTGA CATTGCTCAG TGCAATCTGG TCCTCCTATA TGAGTATGTG 
GGCAATGTCA TCAAAATGAT CCAAACCAGA GGCAGAGGAA GAGCAAGAGG GAGCAAGTGC 
TTCCTTCTGA CTAGTAATNN GATGAATGAC TCCATTTTAA GCCTGCAGAC GTGAGATGAA 
GCAGTATTTA AAGAAAAGGT TTGCCAGATA CAGATTCATG AAAAATTCAT CAGGGATAAT 
CAAGGAAAAG CAAAACCTGG GCTTGATAAG GAAAATAAAA AACTGCTCTG CAGAAAGTGC 
AAAACCTTTG CATGTTATAC AGTTGATATA AGAGTGATGG AGGAATGCCA TTTCATTGTG 
GTTGGAGTTG CTTTCAGGGA GCACTTTGTG AGTAAGTTAC ACCCCAGACC AAAGAGCTTT 
GGGAGTTTTG AGAAGAGAGC AAAGATCTTC TGTGCCAGGG AGGACTGCAG CTATGACTGG 
GGAATCCATG GGAAGTATAA GGTATTTGAG ATTCCAGTTA TAAAGATTCA AAGTTTTGTG 
GTGGAAGATG TTGCAACTGG AGCTCAGACA CTGTATGCAA AGTGGAAGGA CTTTCATTTT 
GAGACCATCC CATTTGATGC TGCAGAAATG TCCACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028020959.1
CDS	155..2893
Translation	MTAEQLQSLHAFRDHVRNFVDSTYILSYVTPWFRDDEVQHIQAEKNNKGPMEAASLFLQFLLELQEESWFCSFLDALDQAGYSGLDDAIESWDFQKIERLEEYRLLLKHLQPEFKSTINPKGILPEISERLINQECEEIIQICSIKGLMASAEKMVECLLRSDKENWPKTLKLALEKEESKFSELWMVEKGAKDVKMKDLEDDEMKTLDVQIFCREEPENQNLSQNSCPSSEVPHTCSPLKPRNYQLELALPAQKGKNTIICAPTGCGKTFVSLLICEHHLKKFPQGQKGKVVFFAIQLPVYEQQKSVFSKYFERLGYKVGGISGVTSDSISVEQTVENNDIIILTPQILVNSLKNGTIPSLSVFTLMIFDECHNTSKHHPYNMIMFNYLDQKLGGSSDSLPQVMGLTASVGTGDAKNTAEATEYFCKLCASLDTSVVATVKDNLEELEEIVYKPQKFFRKVESRTTDRFKCNISQLMVETELARWHNEKLFFFSENLSQIQNRNFGTQRYEQWIIAAQKVRMVFQMPnullDEERRICKAQFFYTSRFNRYNDALIINERARMKNALDYLKDFFRNVRAAGFDEIEQALTQRFEEKLQELESISMDPSNENPKLKDLCFILQEEYHLSPETRIILFGKNKALVDALKKWIEENPKLSFLKPGILTGRGRTNQKIGMTLPVQKCVLDAFRTDGDNKILIATSVADEGIDIAQCNLVLLYEYVGNVIKMIQTRGRGRARGSKCFLLTSNnullMNDSILSLQTDEAVFKEKVCQIQIHEKFIRDNQGKAKPGLDKENKKLLCRKCKTFACYTVDIRVMEECHFIVVGVAFREHFVSKLHPRPKSFGSFEKRAKIFCAREDCSYDWGIHGKYKVFEIPVIKIQSFVVEDVATGAQTLYAKWKDFHFETIPFDAAEMST

Target ORF information:

RefSeq Version	XM_028165158.1
Organism	Balaenoptera acutorostrata scammoni
Definition	Balaenoptera acutorostrata scammoni DExD/H-box helicase 58 (DDX58), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028165158.1

ORF Insert Sequence:

ATGACCGCCG AGCAGCTGCA GAGTCTGCAT GCCTTCCGGG ACCATGTCAG GAACTTCGTG 
GACTCTACCT ACATCCTGAG CTACGTGACG CCCTGGTTTA GGGACGATGA GGTGCAGCAT 
ATTCAGGCTG AGAAAAACAA CAAGGGCCCA ATGGAGGCTG CCTCACTTTT TCTCCAGTTC 
CTGTTGGAGC TCCAGGAGGA AAGCTGGTTC TGCAGCTTTT TGGATGCCCT TGACCAAGCA 
GGTTATTCTG GACTTGATGA TGCCATTGAA AGTTGGGATT TCCAAAAAAT TGAAAGGTTG 
GAGGAGTATA GATTACTTTT AAAGCATTTA CAACCAGAAT TTAAATCCAC GATCAATCCA 
AAAGGTATCC TTCCTGAAAT ATCTGAACGT TTAATTAATC AGGAATGTGA AGAAATTATT 
CAGATTTGTT CCATCAAGGG GCTGATGGCA AGTGCAGAGA AAATGGTTGA ATGCCTTCTC 
AGATCAGACA AGGAGAACTG GCCCAAAACT TTGAAACTTG CATTGGAGAA AGAAGAGAGC 
AAGTTCAGTG AACTGTGGAT GGTAGAGAAA GGTGCAAAAG ATGTCAAAAT GAAAGATCTT 
GAGGATGATG AAATGAAAAC TTTGGATGTA CAGATTTTCT GCAGGGAAGA ACCAGAAAAC 
CAGAATCTTA GTCAGAATTC ATGCCCTTCT TCAGAAGTGC CTCATACTTG CAGCCCATTG 
AAGCCAAGAA ATTACCAACT AGAGCTTGCT TTACCTGCTC AGAAAGGAAA AAACACAATA 
ATATGTGCTC CTACTGGTTG TGGAAAAACC TTTGTTTCAC TTCTTATATG TGAACATCAT 
CTTAAAAAAT TTCCACAAGG ACAAAAGGGG AAAGTTGTCT TTTTTGCTAT TCAACTCCCA 
GTGTATGAGC AGCAGAAATC TGTGTTCTCA AAGTATTTTG AAAGACTTGG GTACAAAGTT 
GGAGGCATTT CTGGAGTAAC ATCTGACAGT ATCTCAGTGG AACAGACTGT TGAGAACAAT 
GACATCATCA TTTTAACTCC ACAGATTCTT GTGAACAGCC TTAAAAATGG GACGATTCCA 
TCACTCTCTG TCTTTACTTT GATGATATTT GATGAATGCC ACAACACTAG TAAACATCAT 
CCTTACAATA TGATCATGTT TAATTATCTA GATCAGAAAC TTGGAGGATC TTCAGACTCA 
TTGCCCCAGG TCATGGGGCT GACTGCCTCG GTTGGCACTG GGGATGCCAA AAATACAGCT 
GAAGCCACAG AATATTTCTG CAAATTGTGT GCTTCTCTTG ACACATCAGT GGTAGCAACA 
GTCAAAGACA ACTTGGAGGA ACTGGAGGAG ATTGTTTATA AGCCCCAGAA GTTTTTCAGG 
AAAGTGGAAT CACGGACCAC CGACAGATTT AAATGCAACA TATCTCAGTT GATGGTAGAG 
ACAGAATGAC TGGCCAGATG GCACAATGAA AAACTTTTTT TTTTTTCAGA AAACTTATCT 
CAAATTCAAA ATAGGAATTT TGGAACACAG AGATATGAAC AGTGGATTAT CGCAGCTCAG 
AAAGTGCGCA TGGTGTTTCA GATGCCANAA GATGAAGAGA GAAGGATTTG TAAAGCACAG 
TTTTTCTACA CGTCGCGTTT CAACAGATAC AATGATGCCC TCATTATCAA TGAGCGTGCT 
CGAATGAAAA ATGCTCTGGA TTACTTAAAA GACTTCTTCA GAAATGTCCG AGCAGCAGGA 
TTTGATGAGA TTGAGCAAGC TCTCACTCAG AGATTTGAAG AAAAGCTGCA GGAACTAGAA 
AGTATTTCCA TGGATCCCAG CAATGAGAAC CCTAAACTCA AAGATCTCTG CTTCATCTTG 
CAAGAAGAGT ACCACTTAAG CCCAGAGACC AGAATCATCC TCTTTGGGAA AAACAAAGCA 
CTCGTGGATG CTTTAAAGAA ATGGATTGAA GAAAATCCTA AGCTCAGCTT TCTAAAACCT 
GGCATATTGA CTGGACGTGG CAGAACAAAT CAGAAAATAG GAATGACCCT CCCAGTACAG 
AAGTGTGTAT TGGATGCATT CAGAACCGAC GGAGATAACA AGATTCTGAT TGCCACCTCG 
GTTGCTGATG AAGGTATTGA CATTGCTCAG TGCAATCTGG TCCTCCTATA TGAGTATGTG 
GGCAATGTCA TCAAAATGAT CCAAACCAGA GGCAGAGGAA GAGCAAGAGG GAGCAAGTGC 
TTCCTTCTGA CTAGTAATNN GATGAATGAC TCCATTTTAA GCCTGCAGAC GTGAGATGAA 
GCAGTATTTA AAGAAAAGGT TTGCCAGATA CAGATTCATG AAAAATTCAT CAGGGATAAT 
CAAGGAAAAG CAAAACCTGG GCTTGATAAG GAAAATAAAA AACTGCTCTG CAGAAAGTGC 
AAAACCTTTG CATGTTATAC AGTTGATATA AGAGTGATGG AGGAATGCCA TTTCATTGTG 
GTTGGAGTTG CTTTCAGGGA GCACTTTGTG AGTAAGTTAC ACCCCAGACC AAAGAGCTTT 
GGGAGTTTTG AGAAGAGAGC AAAGATCTTC TGTGCCAGGG AGGACTGCAG CTATGACTGG 
GGAATCCATG GGAAGTATAA GGTATTTGAG ATTCCAGTTA TAAAGATTCA AAGTTTTGTG 
GTGGAAGATG TTGCAACTGG AGCTCAGACA CTGTATGCAA AGTGGAAGGA CTTTCATTTT 
GAGACCATCC CATTTGATGC TGCAGAAATG TCCACTTGA