crim1 cDNA ORF clone, Austrofundulus limnaeus

Gene
Clones
gRNAs

Summary

Gene Symbol	crim1
Entrez Gene ID	106534335
Full Name	cysteine rich transmembrane BMP regulator 1
Gene Type	protein-coding
Organism	Austrofundulus limnaeus

mRNA and Protein(s)

mRNA	Protein	Name
XM_014030945.1	XP_013886399.1	cysteine-rich motor neuron 1 protein isoform X2
XM_014030944.1	XP_013886398.1	cysteine-rich motor neuron 1 protein isoform X1

Pathways from BioSystems
Gene Ontology

The following crim1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crim1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAu26876	XM_014030944.1 Latest version!	Austrofundulus limnaeus cysteine rich transmembrane BMP regulator 1 (chordin-like) (crim1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OAu26877	XM_014030945.1 Latest version!	Austrofundulus limnaeus cysteine rich transmembrane BMP regulator 1 (chordin-like) (crim1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crim1 ( XM_014030944.1 ) cDNA ORF clone, Austrofundulus limnaeus -> XP_013886398.1 Austrofundulus limnaeus cysteine rich transmembrane BMP regulator 1 (chordin-like) (crim1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAu26876
Clone ID Related Accession (Same CDS sequence)	XM_014030944.1
Accession Version	XM_014030944.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2826bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Austrofundulus limnaeus
Product	cysteine-rich motor neuron 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013953549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Austrofundulus limnaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCGGCC ACGGCTCCGA TGTTTCTCTG ATTGTCCTCT TTCTGGTGCG CGCGCTCCTG 
GCGCACGAGT GCACCCCTTG TAACCTCCGG ACTTGCCCGG TCAAAGCCCC GCGCGGTGCC 
TGCGAGGGTG GCCGGGGTTT GGCGAGGGAT CCGTGCGGAT GCTGCGATCA GTGCGCGCGG 
CTGGAGTGGG AAACGTGCGG CGGGCCGGAC TGGACGCACG GATACTGCGC CCTGGGACTG 
ACCTGCGCCT CTGTCAACAC CACAGGAGCT GCCACCATCC CGGAAACTGG AGTGTGTAAA 
GTACTTCCTG ACCATCCTGA TGCAGGCCTT GAGGATGAGC GCTGTCCGCT ATTGACAGGC 
TGCGACAGAG CTGGGGGTCA GTGTGTGTGC GATGCACGTC GTTCCTGCTT GGGAACTTTC 
GCCTATCCGA ACCAAGAAAC TTGTCTGAAG GCCAGCAAGT CAGACGGTCG GAGGCACGAG 
CACCGGGACA GACACAGAGA GAAGTACGTC GGGCCGGCCA ACCCGGCGTG CACGTTCTCC 
GGGTGCAACC TGACTGCCGA GGGCTGCGTT TGTGAGTCGC AGAGCTGCCA CCACCACTTT 
GCCTACATCA ACCGCAGCCA GTGCCAGGAA GCAGCAGAAC AGCTGAGGTG TGCTGGGGTG 
ACCTGTCCGG CTCTGTCAGT GCCCTCCTGC CCCAAAGGGT CAGTCCTGAC CAAGAGTTAC 
ACGCCCCCTG CTGGTTGCTG CCCTTCCATA CCGCCCCAGT GCACTTGTGA CTTTGGTGCC 
TGCCAGAAGC CCCACTGCCC GAGCGGACAG CACTTTGTTC CACTCAGCCA TGCCAGCGGT 
CAGCCAGGAG ACTGCTGCGA TGTCTACGAG TGCCAGAAAG TCTCTCCAAA GTGTGTCTTC 
AAGGGGAAAG AGTTCTCTGA TGGAGAGGTG TACCGCATGG ACCCCTGCTG GCTGTGTCAG 
TGCCGCGGAG GAATCTCCTT CTGCTCCAAG GCAGAATGTG CCGAGCTAGA ATGTGAAAAC 
TTCTACGTAC CCGAGGGAGA GTGCTGCCCT GTCTGCATAG ATGTAGAGCT GTTGTCATCA 
GACAGCACAA AAGCTAGCTG TTGGATGAAT AATAAGCTGC GAGTTCACGA GGAGCAGTGG 
AAAGAGGACG ACTGCACCTT CTGTCAGTGT GTAGAYGGGG ATCCTCACTG TACAGCGATG 
GCCTGCAAAC AGAGCTGCCA AAACCCTGTG AAGATCCCTG GCGAGTGCTG TCCCTTCTGC 
GAAGAACCTT CTTATGAGAC TGTTAGCCCC ATTTTGTGCC CACCTCTGGA AAACTGCTCC 
CTGTCAGTGA ACGACTGCCC TCTTGGCTTC CATCAGGACA ACAATGGGTG TCTCCTGTGC 
CAGTGCCTCA ACAATGACTC CTGTCCTGAC TTGGGAAAAT ACTGTTYTCT GCAATGTCCA 
ACGGGATACG AGAAGGATGA TTTTGGTTGT GAGGTGTGTG AGTGCAGCAT CCCCACGCCC 
AAGTGCCGAC CTCTGACCTG CACAAAGACC TGTCCCTACG GATATGTGAG GAACAAGCAC 
GGCTGCGAGA TGTGCCGCTG CGTCAAATGT CCTCCCTTCA CCTGCGACAA GCACTGCAGC 
AACGGCTATC GACAGAACCG CAAGGGCTGC TCCGTCTGCA TGTGCAACGA AAGCAGCCAG 
GTTGCCACCA CCACCACCAC CACCATCCCA GCCCCAACGC CCAGCTACTG CCTCACCTCC 
AACGGGCGCC TTTACGAGGA GGGTGACAGC TGGCACGATG GCTGCCGGGA CTGCTTCTGC 
CACTCGGGAC GGGAGATGTG CGTGCTCATA TCCTGCCCCA TGCCCAGCTG CCCTCATCCT 
GTTGTCAAGC CTGACCAGTG TTGTCCCACC TGTGAGGATG AGTCCGGCAG CGGCCAGCCA 
GAGGTGGTCT GCAGAGCACC TGGCGGGGAG TTTTACGTGG AGGGGGAGAC CTGGAACCTG 
GACGAGTGCA CACGCTGCAC CTGCAGGAAG GGCCGCGTCC TGTGCGACAC CGAAGCATGT 
CCACCGGCTC TCTGTCAGAC CCCCATCAGG AACAAGGACA CCTGCTGCCA TGTGTGCCCA 
GAGGAGACGC TGAGTCCTTT GCTGCCAGTA AACAGCAGCC AGCAGCAGTA CTGCATGACC 
AGCGAGGGGG ACGTGCTGCT AGCAGGGGAC TCCTGGAAGA CCAACGCCTG CACCAGCTGC 
ACCTGCAACA ACGGAACCAT TCAGTGTTTC TCTCAGCGCT GCCCGGCTGC CAACTGCAGA 
GTGCCGGTGC TACGCAAGGG CCAGTGTTGC CCACACTGTC TCGGTGAGTT CATAGATGTG 
ACGACGTCGT TGCCAGTGAT AGTGTCCACC AGCTCTCCGA AGACAGAGGC CACCACTGCA 
GGGGGGGACG CACCGCGGAT CACCACAGTC ACTGTCCCAC TTGTTATTGC TGACACAGAT 
GAGCCAGGTT TGGGTGAGAA CTACCCCAGT CAAACAGAGA TGGCCCTTGT TTACCAATCR 
GCTGCCTGGA TCCTGGCTGG CCTCCTCCTG GCCATCATCA TCTTCCTCAT CGTGGCRTTG 
CTCATCAACA AGAAGAAGAA GTGGGTGCAG ATGTCCTGCT ACAGCGCACC CAAGAAGGCG 
GTGATCCTGA AAAAGCACAT AAACAAGAAC TCCGTCATCT ACATGGAGCC CTCGAAGGAG 
AACAAGTTTC AGAACGTGAA GAACGACTGC GGCATCATCC ACTTCGCTCC AGACATCAGC 
TCCCCTGACA AGATGACCAT CCCCAGGGCC AAGCTGAGCC TGAACAACGA CTGGACGCCC 
CGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013886398.1
CDS	375..3200
Translation	MFGHGSDVSLIVLFLVRALLAHECTPCNLRTCPVKAPRGACEGGRGLARDPCGCCDQCARLEWETCGGPDWTHGYCALGLTCASVNTTGAATIPETGVCKVLPDHPDAGLEDERCPLLTGCDRAGGQCVCDARRSCLGTFAYPNQETCLKASKSDGRRHEHRDRHREKYVGPANPACTFSGCNLTAEGCVCESQSCHHHFAYINRSQCQEAAEQLRCAGVTCPALSVPSCPKGSVLTKSYTPPAGCCPSIPPQCTCDFGACQKPHCPSGQHFVPLSHASGQPGDCCDVYECQKVSPKCVFKGKEFSDGEVYRMDPCWLCQCRGGISFCSKAECAELECENFYVPEGECCPVCIDVELLSSDSTKASCWMNNKLRVHEEQWKEDDCTFCQCVnullGDPHCTAMACKQSCQNPVKIPGECCPFCEEPSYETVSPILCPPLENCSLSVNDCPLGFHQDNNGCLLCQCLNNDSCPDLGKYCnullLQCPTGYEKDDFGCEVCECSIPTPKCRPLTCTKTCPYGYVRNKHGCEMCRCVKCPPFTCDKHCSNGYRQNRKGCSVCMCNESSQVATTTTTTIPAPTPSYCLTSNGRLYEEGDSWHDGCRDCFCHSGREMCVLISCPMPSCPHPVVKPDQCCPTCEDESGSGQPEVVCRAPGGEFYVEGETWNLDECTRCTCRKGRVLCDTEACPPALCQTPIRNKDTCCHVCPEETLSPLLPVNSSQQQYCMTSEGDVLLAGDSWKTNACTSCTCNNGTIQCFSQRCPAANCRVPVLRKGQCCPHCLGEFIDVTTSLPVIVSTSSPKTEATTAGGDAPRITTVTVPLVIADTDEPGLGENYPSQTEMALVYQnullAAWILAGLLLAIIIFLIVnullLLINKKKKWVQMSCYSAPKKAVILKKHINKNSVIYMEPSKENKFQNVKNDCGIIHFAPDISSPDKMTIPRAKLSLNNDWTPR

Target ORF information:

RefSeq Version	XM_014030944.1
Organism	Austrofundulus limnaeus
Definition	Austrofundulus limnaeus cysteine rich transmembrane BMP regulator 1 (chordin-like) (crim1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014030944.1

ORF Insert Sequence:

ATGTTCGGCC ACGGCTCCGA TGTTTCTCTG ATTGTCCTCT TTCTGGTGCG CGCGCTCCTG 
GCGCACGAGT GCACCCCTTG TAACCTCCGG ACTTGCCCGG TCAAAGCCCC GCGCGGTGCC 
TGCGAGGGTG GCCGGGGTTT GGCGAGGGAT CCGTGCGGAT GCTGCGATCA GTGCGCGCGG 
CTGGAGTGGG AAACGTGCGG CGGGCCGGAC TGGACGCACG GATACTGCGC CCTGGGACTG 
ACCTGCGCCT CTGTCAACAC CACAGGAGCT GCCACCATCC CGGAAACTGG AGTGTGTAAA 
GTACTTCCTG ACCATCCTGA TGCAGGCCTT GAGGATGAGC GCTGTCCGCT ATTGACAGGC 
TGCGACAGAG CTGGGGGTCA GTGTGTGTGC GATGCACGTC GTTCCTGCTT GGGAACTTTC 
GCCTATCCGA ACCAAGAAAC TTGTCTGAAG GCCAGCAAGT CAGACGGTCG GAGGCACGAG 
CACCGGGACA GACACAGAGA GAAGTACGTC GGGCCGGCCA ACCCGGCGTG CACGTTCTCC 
GGGTGCAACC TGACTGCCGA GGGCTGCGTT TGTGAGTCGC AGAGCTGCCA CCACCACTTT 
GCCTACATCA ACCGCAGCCA GTGCCAGGAA GCAGCAGAAC AGCTGAGGTG TGCTGGGGTG 
ACCTGTCCGG CTCTGTCAGT GCCCTCCTGC CCCAAAGGGT CAGTCCTGAC CAAGAGTTAC 
ACGCCCCCTG CTGGTTGCTG CCCTTCCATA CCGCCCCAGT GCACTTGTGA CTTTGGTGCC 
TGCCAGAAGC CCCACTGCCC GAGCGGACAG CACTTTGTTC CACTCAGCCA TGCCAGCGGT 
CAGCCAGGAG ACTGCTGCGA TGTCTACGAG TGCCAGAAAG TCTCTCCAAA GTGTGTCTTC 
AAGGGGAAAG AGTTCTCTGA TGGAGAGGTG TACCGCATGG ACCCCTGCTG GCTGTGTCAG 
TGCCGCGGAG GAATCTCCTT CTGCTCCAAG GCAGAATGTG CCGAGCTAGA ATGTGAAAAC 
TTCTACGTAC CCGAGGGAGA GTGCTGCCCT GTCTGCATAG ATGTAGAGCT GTTGTCATCA 
GACAGCACAA AAGCTAGCTG TTGGATGAAT AATAAGCTGC GAGTTCACGA GGAGCAGTGG 
AAAGAGGACG ACTGCACCTT CTGTCAGTGT GTAGAYGGGG ATCCTCACTG TACAGCGATG 
GCCTGCAAAC AGAGCTGCCA AAACCCTGTG AAGATCCCTG GCGAGTGCTG TCCCTTCTGC 
GAAGAACCTT CTTATGAGAC TGTTAGCCCC ATTTTGTGCC CACCTCTGGA AAACTGCTCC 
CTGTCAGTGA ACGACTGCCC TCTTGGCTTC CATCAGGACA ACAATGGGTG TCTCCTGTGC 
CAGTGCCTCA ACAATGACTC CTGTCCTGAC TTGGGAAAAT ACTGTTYTCT GCAATGTCCA 
ACGGGATACG AGAAGGATGA TTTTGGTTGT GAGGTGTGTG AGTGCAGCAT CCCCACGCCC 
AAGTGCCGAC CTCTGACCTG CACAAAGACC TGTCCCTACG GATATGTGAG GAACAAGCAC 
GGCTGCGAGA TGTGCCGCTG CGTCAAATGT CCTCCCTTCA CCTGCGACAA GCACTGCAGC 
AACGGCTATC GACAGAACCG CAAGGGCTGC TCCGTCTGCA TGTGCAACGA AAGCAGCCAG 
GTTGCCACCA CCACCACCAC CACCATCCCA GCCCCAACGC CCAGCTACTG CCTCACCTCC 
AACGGGCGCC TTTACGAGGA GGGTGACAGC TGGCACGATG GCTGCCGGGA CTGCTTCTGC 
CACTCGGGAC GGGAGATGTG CGTGCTCATA TCCTGCCCCA TGCCCAGCTG CCCTCATCCT 
GTTGTCAAGC CTGACCAGTG TTGTCCCACC TGTGAGGATG AGTCCGGCAG CGGCCAGCCA 
GAGGTGGTCT GCAGAGCACC TGGCGGGGAG TTTTACGTGG AGGGGGAGAC CTGGAACCTG 
GACGAGTGCA CACGCTGCAC CTGCAGGAAG GGCCGCGTCC TGTGCGACAC CGAAGCATGT 
CCACCGGCTC TCTGTCAGAC CCCCATCAGG AACAAGGACA CCTGCTGCCA TGTGTGCCCA 
GAGGAGACGC TGAGTCCTTT GCTGCCAGTA AACAGCAGCC AGCAGCAGTA CTGCATGACC 
AGCGAGGGGG ACGTGCTGCT AGCAGGGGAC TCCTGGAAGA CCAACGCCTG CACCAGCTGC 
ACCTGCAACA ACGGAACCAT TCAGTGTTTC TCTCAGCGCT GCCCGGCTGC CAACTGCAGA 
GTGCCGGTGC TACGCAAGGG CCAGTGTTGC CCACACTGTC TCGGTGAGTT CATAGATGTG 
ACGACGTCGT TGCCAGTGAT AGTGTCCACC AGCTCTCCGA AGACAGAGGC CACCACTGCA 
GGGGGGGACG CACCGCGGAT CACCACAGTC ACTGTCCCAC TTGTTATTGC TGACACAGAT 
GAGCCAGGTT TGGGTGAGAA CTACCCCAGT CAAACAGAGA TGGCCCTTGT TTACCAATCR 
GCTGCCTGGA TCCTGGCTGG CCTCCTCCTG GCCATCATCA TCTTCCTCAT CGTGGCRTTG 
CTCATCAACA AGAAGAAGAA GTGGGTGCAG ATGTCCTGCT ACAGCGCACC CAAGAAGGCG 
GTGATCCTGA AAAAGCACAT AAACAAGAAC TCCGTCATCT ACATGGAGCC CTCGAAGGAG 
AACAAGTTTC AGAACGTGAA GAACGACTGC GGCATCATCC ACTTCGCTCC AGACATCAGC 
TCCCCTGACA AGATGACCAT CCCCAGGGCC AAGCTGAGCC TGAACAACGA CTGGACGCCC 
CGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

crim1 ( XM_014030945.1 ) cDNA ORF clone, Austrofundulus limnaeus -> XP_013886399.1 Austrofundulus limnaeus cysteine rich transmembrane BMP regulator 1 (chordin-like) (crim1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAu26877
Clone ID Related Accession (Same CDS sequence)	XM_014030945.1
Accession Version	XM_014030945.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Austrofundulus limnaeus
Product	cysteine-rich motor neuron 1 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013953549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Austrofundulus limnaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCGGCC ACGGCTCCGA TGTTTCTCTG ATTGTCCTCT TTCTGGTGCG CGCGCTCCTG 
GCGCACGAGT GCACCCCTTG TAACCTCCGG ACTTGCCCGG TCAAAGCCCC GCGCGGTGCC 
TGCGAGGGTG GCCGGGGTTT GGCGAGGGAT CCGTGCGGAT GCTGCGATCA GTGCGCGCGG 
CTGGAGTGGG AAACGTGCGG CGGGCCGGAC TGGACGCACG GATACTGCGC CCTGGGACTG 
ACCTGCGCCT CTGTCAACAC CACAGGAGCT GCCACCATCC CGGAAACTGG AGTGTGTAAA 
GTACTTCCTG ACCATCCTGA TGCAGGCCTT GAGGATGAGC GCTGTCCGCT ATTGACAGGC 
TGCGACAGAG CTGGGGGTCA GTGTGTGTGC GATGCACGTC GTTCCTGCTT GGGAACTTTC 
GCCTATCCGA ACCAAGAAAC TTGTCTGAAG GCCAGCAAGT CAGACGGTCG GAGGCACGAG 
CACCGGGACA GACACAGAGA GAAGTACGTC GGGCCGGCCA ACCCGGCGTG CACGTTCTCC 
GGGTGCAACC TGACTGCCGA GGGCTGCGTT TGTGAGTCGC AGAGCTGCCA CCACCACTTT 
GCCTACATCA ACCGCAGCCA GTGCCAGGAA GCAGCAGAAC AGCTGAGGTG TGCTGGGGTG 
ACCTGTCCGG CTCTGTCAGT GCCCTCCTGC CCCAAAGGGT CAGTCCTGAC CAAGAGTTAC 
ACGCCCCCTG CTGGTTGCTG CCCTTCCATA CCGCCCCAGT GCACTTGTGA CTTTGGTGCC 
TGCCAGAAGC CCCACTGCCC GAGCGGACAG CACTTTGTTC CACTCAGCCA TGCCAGCGGT 
CAGCCAGGAG ACTGCTGCGA TGTCTACGAG TGCCAGAAAG TCTCTCCAAA GTGTGTCTTC 
AAGGGGAAAG AGTTCTCTGA TGGAGAGGTG TACCGCATGG ACCCCTGCTG GCTGTGTCAG 
TGCCGCGGAG GAATCTCCTT CTGCTCCAAG GCAGAATGTG CCGAGCTAGA ATGTGAAAAC 
TTCTACGTAC CCGAGGGAGA GTGCTGCCCT GTCTGCATAG ATGTAGAGCT GTTGTCATCA 
GACAGCACAA AAGCTAGCTG TTGGATGAAT AATAAGCTGC GAGTTCACGA GGAGCAGTGG 
AAAGAGGACG ACTGCACCTT CTGTCAGTGT GTAGAYGGGG ATCCTCACTG TACAGCGATG 
GCCTGCAAAC AGAGCTGCCA AAACCCTGTG AAGATCCCTG GCGAGTGCTG TCCCTTCTGC 
GAAGAACCTT CTTATGAGAC TGTTAGCCCC ATTTTGTGCC CACCTCTGGA AAACTGCTCC 
CTGTCAGTGA ACGACTGCCC TCTTGGCTTC CATCAGGACA ACAATGGGTG TCTCCTGTGC 
CAGTGCCTCA ACAATGACTC CTGTCCTGAC TTGGGAAAAT ACTGTTYTCT GCAATGTCCA 
ACGGGATACG AGAAGGATGA TTTTGGTTGT GAGGTGTGTG AGTGCAGCAT CCCCACGCCC 
AAGTGCCGAC CTCTGACCTG CACAAAGACC TGTCCCTACG GATATGTGAG GAACAAGCAC 
GGCTGCGAGA TGTGCCGCTG CGTCAAATGT CCTCCCTTCA CCTGCGACAA GCACTGCAGC 
AACGGCTATC GACAGAACCG CAAGGGCTGC TCCGTCTGCA TGTGCAACGA AAGCAGCCAG 
GTTGCCACCA CCACCACCAC CACCATCCCA GCCCCAACGC CCAGCTACTG CCTCACCTCC 
AACGGGCGCC TTTACGAGGA GGGTGACAGC TGGCACGATG GCTGCCGGGA CTGCTTCTGC 
CACTCGGGAC GGGAGATGTG CGTGCTCATA TCCTGCCCCA TGCCCAGCTG CCCTCATCCT 
GTTGTCAAGC CTGACCAGTG TTGTCCCACC TGTGAGGATG AGTCCGGCAG CGGCCAGCCA 
GAGGTGGTCT GCAGAGCACC TGGCGGGGAG TTTTACGTGG AGGGGGAGAC CTGGAACCTG 
GACGAGTGCA CACGCTGCAC CTGCAGGAAG GGCCGCGTCC TGTGCGACAC CGAAGCATGT 
CCACCGGCTC TCTGTCAGAC CCCCATCAGG AACAAGGACA CCTGCTGCCA TGTGTGCCCA 
GAGGAGACGC TGAGTCCTTT GCTGCCAGTA AACAGCAGCC AGCAGCAGTA CTGCATGACC 
AGCGAGGGGG ACGTGCTGCT AGCAGGGGAC TCCTGGAAGA CCAACGCCTG CACCAGCTGC 
ACCTGCAACA ACGGAACCAT TCAGTGTTTC TCTCAGCGCT GCCCGGCTGC CAACTGCAGA 
GTGCCGGTGC TACGCAAGGG CCAGTGTTGC CCACACTGTC TCGATGTGAC GACGTCGTTG 
CCAGTGATAG TGTCCACCAG CTCTCCGAAG ACAGAGGCCA CCACTGCAGG GGGGGACGCA 
CCGCGGATCA CCACAGTCAC TGTCCCACTT GTTATTGCTG ACACAGATGA GCCAGGTTTG 
GGTGAGAACT ACCCCAGTCA AACAGAGATG GCCCTTGTTT ACCAATCRGC TGCCTGGATC 
CTGGCTGGCC TCCTCCTGGC CATCATCATC TTCCTCATCG TGGCRTTGCT CATCAACAAG 
AAGAAGAAGT GGGTGCAGAT GTCCTGCTAC AGCGCACCCA AGAAGGCGGT GATCCTGAAA 
AAGCACATAA ACAAGAACTC CGTCATCTAC ATGGAGCCCT CGAAGGAGAA CAAGTTTCAG 
AACGTGAAGA ACGACTGCGG CATCATCCAC TTCGCTCCAG ACATCAGCTC CCCTGACAAG 
ATGACCATCC CCAGGGCCAA GCTGAGCCTG AACAACGACT GGACGCCCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013886399.1
CDS	374..3187
Translation	MFGHGSDVSLIVLFLVRALLAHECTPCNLRTCPVKAPRGACEGGRGLARDPCGCCDQCARLEWETCGGPDWTHGYCALGLTCASVNTTGAATIPETGVCKVLPDHPDAGLEDERCPLLTGCDRAGGQCVCDARRSCLGTFAYPNQETCLKASKSDGRRHEHRDRHREKYVGPANPACTFSGCNLTAEGCVCESQSCHHHFAYINRSQCQEAAEQLRCAGVTCPALSVPSCPKGSVLTKSYTPPAGCCPSIPPQCTCDFGACQKPHCPSGQHFVPLSHASGQPGDCCDVYECQKVSPKCVFKGKEFSDGEVYRMDPCWLCQCRGGISFCSKAECAELECENFYVPEGECCPVCIDVELLSSDSTKASCWMNNKLRVHEEQWKEDDCTFCQCVnullGDPHCTAMACKQSCQNPVKIPGECCPFCEEPSYETVSPILCPPLENCSLSVNDCPLGFHQDNNGCLLCQCLNNDSCPDLGKYCnullLQCPTGYEKDDFGCEVCECSIPTPKCRPLTCTKTCPYGYVRNKHGCEMCRCVKCPPFTCDKHCSNGYRQNRKGCSVCMCNESSQVATTTTTTIPAPTPSYCLTSNGRLYEEGDSWHDGCRDCFCHSGREMCVLISCPMPSCPHPVVKPDQCCPTCEDESGSGQPEVVCRAPGGEFYVEGETWNLDECTRCTCRKGRVLCDTEACPPALCQTPIRNKDTCCHVCPEETLSPLLPVNSSQQQYCMTSEGDVLLAGDSWKTNACTSCTCNNGTIQCFSQRCPAANCRVPVLRKGQCCPHCLDVTTSLPVIVSTSSPKTEATTAGGDAPRITTVTVPLVIADTDEPGLGENYPSQTEMALVYQnullAAWILAGLLLAIIIFLIVnullLLINKKKKWVQMSCYSAPKKAVILKKHINKNSVIYMEPSKENKFQNVKNDCGIIHFAPDISSPDKMTIPRAKLSLNNDWTPR

Target ORF information:

RefSeq Version	XM_014030945.1
Organism	Austrofundulus limnaeus
Definition	Austrofundulus limnaeus cysteine rich transmembrane BMP regulator 1 (chordin-like) (crim1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014030945.1

ORF Insert Sequence:

ATGTTCGGCC ACGGCTCCGA TGTTTCTCTG ATTGTCCTCT TTCTGGTGCG CGCGCTCCTG 
GCGCACGAGT GCACCCCTTG TAACCTCCGG ACTTGCCCGG TCAAAGCCCC GCGCGGTGCC 
TGCGAGGGTG GCCGGGGTTT GGCGAGGGAT CCGTGCGGAT GCTGCGATCA GTGCGCGCGG 
CTGGAGTGGG AAACGTGCGG CGGGCCGGAC TGGACGCACG GATACTGCGC CCTGGGACTG 
ACCTGCGCCT CTGTCAACAC CACAGGAGCT GCCACCATCC CGGAAACTGG AGTGTGTAAA 
GTACTTCCTG ACCATCCTGA TGCAGGCCTT GAGGATGAGC GCTGTCCGCT ATTGACAGGC 
TGCGACAGAG CTGGGGGTCA GTGTGTGTGC GATGCACGTC GTTCCTGCTT GGGAACTTTC 
GCCTATCCGA ACCAAGAAAC TTGTCTGAAG GCCAGCAAGT CAGACGGTCG GAGGCACGAG 
CACCGGGACA GACACAGAGA GAAGTACGTC GGGCCGGCCA ACCCGGCGTG CACGTTCTCC 
GGGTGCAACC TGACTGCCGA GGGCTGCGTT TGTGAGTCGC AGAGCTGCCA CCACCACTTT 
GCCTACATCA ACCGCAGCCA GTGCCAGGAA GCAGCAGAAC AGCTGAGGTG TGCTGGGGTG 
ACCTGTCCGG CTCTGTCAGT GCCCTCCTGC CCCAAAGGGT CAGTCCTGAC CAAGAGTTAC 
ACGCCCCCTG CTGGTTGCTG CCCTTCCATA CCGCCCCAGT GCACTTGTGA CTTTGGTGCC 
TGCCAGAAGC CCCACTGCCC GAGCGGACAG CACTTTGTTC CACTCAGCCA TGCCAGCGGT 
CAGCCAGGAG ACTGCTGCGA TGTCTACGAG TGCCAGAAAG TCTCTCCAAA GTGTGTCTTC 
AAGGGGAAAG AGTTCTCTGA TGGAGAGGTG TACCGCATGG ACCCCTGCTG GCTGTGTCAG 
TGCCGCGGAG GAATCTCCTT CTGCTCCAAG GCAGAATGTG CCGAGCTAGA ATGTGAAAAC 
TTCTACGTAC CCGAGGGAGA GTGCTGCCCT GTCTGCATAG ATGTAGAGCT GTTGTCATCA 
GACAGCACAA AAGCTAGCTG TTGGATGAAT AATAAGCTGC GAGTTCACGA GGAGCAGTGG 
AAAGAGGACG ACTGCACCTT CTGTCAGTGT GTAGAYGGGG ATCCTCACTG TACAGCGATG 
GCCTGCAAAC AGAGCTGCCA AAACCCTGTG AAGATCCCTG GCGAGTGCTG TCCCTTCTGC 
GAAGAACCTT CTTATGAGAC TGTTAGCCCC ATTTTGTGCC CACCTCTGGA AAACTGCTCC 
CTGTCAGTGA ACGACTGCCC TCTTGGCTTC CATCAGGACA ACAATGGGTG TCTCCTGTGC 
CAGTGCCTCA ACAATGACTC CTGTCCTGAC TTGGGAAAAT ACTGTTYTCT GCAATGTCCA 
ACGGGATACG AGAAGGATGA TTTTGGTTGT GAGGTGTGTG AGTGCAGCAT CCCCACGCCC 
AAGTGCCGAC CTCTGACCTG CACAAAGACC TGTCCCTACG GATATGTGAG GAACAAGCAC 
GGCTGCGAGA TGTGCCGCTG CGTCAAATGT CCTCCCTTCA CCTGCGACAA GCACTGCAGC 
AACGGCTATC GACAGAACCG CAAGGGCTGC TCCGTCTGCA TGTGCAACGA AAGCAGCCAG 
GTTGCCACCA CCACCACCAC CACCATCCCA GCCCCAACGC CCAGCTACTG CCTCACCTCC 
AACGGGCGCC TTTACGAGGA GGGTGACAGC TGGCACGATG GCTGCCGGGA CTGCTTCTGC 
CACTCGGGAC GGGAGATGTG CGTGCTCATA TCCTGCCCCA TGCCCAGCTG CCCTCATCCT 
GTTGTCAAGC CTGACCAGTG TTGTCCCACC TGTGAGGATG AGTCCGGCAG CGGCCAGCCA 
GAGGTGGTCT GCAGAGCACC TGGCGGGGAG TTTTACGTGG AGGGGGAGAC CTGGAACCTG 
GACGAGTGCA CACGCTGCAC CTGCAGGAAG GGCCGCGTCC TGTGCGACAC CGAAGCATGT 
CCACCGGCTC TCTGTCAGAC CCCCATCAGG AACAAGGACA CCTGCTGCCA TGTGTGCCCA 
GAGGAGACGC TGAGTCCTTT GCTGCCAGTA AACAGCAGCC AGCAGCAGTA CTGCATGACC 
AGCGAGGGGG ACGTGCTGCT AGCAGGGGAC TCCTGGAAGA CCAACGCCTG CACCAGCTGC 
ACCTGCAACA ACGGAACCAT TCAGTGTTTC TCTCAGCGCT GCCCGGCTGC CAACTGCAGA 
GTGCCGGTGC TACGCAAGGG CCAGTGTTGC CCACACTGTC TCGATGTGAC GACGTCGTTG 
CCAGTGATAG TGTCCACCAG CTCTCCGAAG ACAGAGGCCA CCACTGCAGG GGGGGACGCA 
CCGCGGATCA CCACAGTCAC TGTCCCACTT GTTATTGCTG ACACAGATGA GCCAGGTTTG 
GGTGAGAACT ACCCCAGTCA AACAGAGATG GCCCTTGTTT ACCAATCRGC TGCCTGGATC 
CTGGCTGGCC TCCTCCTGGC CATCATCATC TTCCTCATCG TGGCRTTGCT CATCAACAAG 
AAGAAGAAGT GGGTGCAGAT GTCCTGCTAC AGCGCACCCA AGAAGGCGGT GATCCTGAAA 
AAGCACATAA ACAAGAACTC CGTCATCTAC ATGGAGCCCT CGAAGGAGAA CAAGTTTCAG 
AACGTGAAGA ACGACTGCGG CATCATCCAC TTCGCTCCAG ACATCAGCTC CCCTGACAAG 
ATGACCATCC CCAGGGCCAA GCTGAGCCTG AACAACGACT GGACGCCCCG TTAA