LOC105686253 cDNA ORF clone, Athalia rosae(coleseed sawfly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105686253
Entrez Gene ID	105686253
Full Name	mRNA cap guanine-N7 methyltransferase
Gene Type	protein-coding
Organism	Athalia rosae(coleseed sawfly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012400905.2	XP_012256328.1	mRNA cap guanine-N7 methyltransferase isoform X2
XM_012400904.2	XP_012256327.1	mRNA cap guanine-N7 methyltransferase isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC105686253 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105686253 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAt100636	XM_012400904.2 Latest version!	Athalia rosae mRNA cap guanine-N7 methyltransferase (LOC105686253), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OAt100637	XM_012400905.2 Latest version!	Athalia rosae mRNA cap guanine-N7 methyltransferase (LOC105686253), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105686253 ( XM_012400904.2 ) cDNA ORF clone, Athalia rosae(coleseed sawfly) -> XP_012256327.1 Athalia rosae mRNA cap guanine-N7 methyltransferase (LOC105686253), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAt100636
Clone ID Related Accession (Same CDS sequence)	XM_012400904.2
Accession Version	XM_012400904.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-16
Organism	Athalia rosae(coleseed sawfly)
Product	mRNA cap guanine-N7 methyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020311966.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 20, 2017 this sequence version replaced XM_012400904.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Athalia rosae Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTCTT CGCCCAAATC GGAAACCGTC ACGAGCGAAG TGCAGGGTAG CCAGGAGTCT 
CAAGACGTGC ACCTTACCAC AATGACATAC ATACATGTTA AGGATACCAG TGCTCCGTCT 
GACCTTTCAA CAGGCAGCAA TAGGGGAGAC GAATATGTTC CCGACAGACA GAAGCCTACC 
AGCAACTCTA ATGTTGTAGC CGAGCACTAC AACATGCTCG AAGAAAAAGG TCTTGGTCAG 
AGATCTCAAA GCCGCATACT TTACATGAGA AATTTCAACA ACTGGGTTAA AAGCATGCTT 
ATTGGTGAGT ACATAGGCAA AGCGCGTCAA GGGAAAGGAC ACAGGGTTCC ATTTAAAGTC 
CTTGACATGT GTTGTGGAAA AGGCGGAGAT CTTTTGAAAT GGTCAAAGGC TAATATCTCG 
CATCTTATTT GCGCTGATAT AGCAGAAGTT TCGATCCAAC AATGCCAGAG CCGCTACAAT 
GACTTATTGA ACAGGAGTTC CAACAACAGA GGATATGCTC CAGCATTTAC TGCGGAGTTT 
ATCACGGCAG ACTGCACCAA GACAAGACTG AGAGAAAAAT ATAAGGATGC TAGCTCTCAG 
CTGGATTTGG TAAGCTGCCA ATTCGCGTTT CACTACAGTT TTGAGTCGTT GCCACAGGCG 
GAGTGCATGC TCAAAAACGC AGGGGAGAGC TTGAGACCCG GAGCGTATTT TATCGGCACC 
ATTCCTGATG CTTATGAGCT AGTGTCTCGA TGGCAGAAAA GCAACTCTGA TAAGATTGGA 
AATGACGTTT ACAACATAGA ATTTTTATGC AAAGACAAAG TAAAACCACC GTTGTTCGGG 
GCCAAATACA ATTTTTATTT GGAGGGAGTG GTCAACTGTC CGGAATATCT CGTGCATTTG 
CCAACTCTCA CAAAACTTGC GTTGAAGTAT GGACTCGAGC TGGTCATGTT TGAAAGGTTT 
GAAAATTTCT TCAAACGCGT AAAGGACGAG GGGAGATCCC TGCTCGGTAA AGTTCAGGCA 
CTCGAGACAT ATCCACCGTG TCACGAGGTG CCACTACTTG GTCAGAATCC CGATGACTAT 
CGCCACGCTT TGCAGTACAT GCAGAATTCG AGCGGACACA GAAAAATTGG TACTCTCTCA 
CTACCCGAGT GGGAGGCGAC CTCACTGTAT GCAGTATTCG CTTTTCAAAA AATGAAGACA 
ACGTGGAATG CCGAAGGAAA ACCAGAGTAT GTTAAACTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012256327.1
CDS	220..1461
Translation	MSSSPKSETVTSEVQGSQESQDVHLTTMTYIHVKDTSAPSDLSTGSNRGDEYVPDRQKPTSNSNVVAEHYNMLEEKGLGQRSQSRILYMRNFNNWVKSMLIGEYIGKARQGKGHRVPFKVLDMCCGKGGDLLKWSKANISHLICADIAEVSIQQCQSRYNDLLNRSSNNRGYAPAFTAEFITADCTKTRLREKYKDASSQLDLVSCQFAFHYSFESLPQAECMLKNAGESLRPGAYFIGTIPDAYELVSRWQKSNSDKIGNDVYNIEFLCKDKVKPPLFGAKYNFYLEGVVNCPEYLVHLPTLTKLALKYGLELVMFERFENFFKRVKDEGRSLLGKVQALETYPPCHEVPLLGQNPDDYRHALQYMQNSSGHRKIGTLSLPEWEATSLYAVFAFQKMKTTWNAEGKPEYVKL

Target ORF information:

RefSeq Version	XM_012400904.2
Organism	Athalia rosae(coleseed sawfly)
Definition	Athalia rosae mRNA cap guanine-N7 methyltransferase (LOC105686253), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012400904.2

ORF Insert Sequence:

ATGTCTTCTT CGCCCAAATC GGAAACCGTC ACGAGCGAAG TGCAGGGTAG CCAGGAGTCT 
CAAGACGTGC ACCTTACCAC AATGACATAC ATACATGTTA AGGATACCAG TGCTCCGTCT 
GACCTTTCAA CAGGCAGCAA TAGGGGAGAC GAATATGTTC CCGACAGACA GAAGCCTACC 
AGCAACTCTA ATGTTGTAGC CGAGCACTAC AACATGCTCG AAGAAAAAGG TCTTGGTCAG 
AGATCTCAAA GCCGCATACT TTACATGAGA AATTTCAACA ACTGGGTTAA AAGCATGCTT 
ATTGGTGAGT ACATAGGCAA AGCGCGTCAA GGGAAAGGAC ACAGGGTTCC ATTTAAAGTC 
CTTGACATGT GTTGTGGAAA AGGCGGAGAT CTTTTGAAAT GGTCAAAGGC TAATATCTCG 
CATCTTATTT GCGCTGATAT AGCAGAAGTT TCGATCCAAC AATGCCAGAG CCGCTACAAT 
GACTTATTGA ACAGGAGTTC CAACAACAGA GGATATGCTC CAGCATTTAC TGCGGAGTTT 
ATCACGGCAG ACTGCACCAA GACAAGACTG AGAGAAAAAT ATAAGGATGC TAGCTCTCAG 
CTGGATTTGG TAAGCTGCCA ATTCGCGTTT CACTACAGTT TTGAGTCGTT GCCACAGGCG 
GAGTGCATGC TCAAAAACGC AGGGGAGAGC TTGAGACCCG GAGCGTATTT TATCGGCACC 
ATTCCTGATG CTTATGAGCT AGTGTCTCGA TGGCAGAAAA GCAACTCTGA TAAGATTGGA 
AATGACGTTT ACAACATAGA ATTTTTATGC AAAGACAAAG TAAAACCACC GTTGTTCGGG 
GCCAAATACA ATTTTTATTT GGAGGGAGTG GTCAACTGTC CGGAATATCT CGTGCATTTG 
CCAACTCTCA CAAAACTTGC GTTGAAGTAT GGACTCGAGC TGGTCATGTT TGAAAGGTTT 
GAAAATTTCT TCAAACGCGT AAAGGACGAG GGGAGATCCC TGCTCGGTAA AGTTCAGGCA 
CTCGAGACAT ATCCACCGTG TCACGAGGTG CCACTACTTG GTCAGAATCC CGATGACTAT 
CGCCACGCTT TGCAGTACAT GCAGAATTCG AGCGGACACA GAAAAATTGG TACTCTCTCA 
CTACCCGAGT GGGAGGCGAC CTCACTGTAT GCAGTATTCG CTTTTCAAAA AATGAAGACA 
ACGTGGAATG CCGAAGGAAA ACCAGAGTAT GTTAAACTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105686253 ( XM_012400905.2 ) cDNA ORF clone, Athalia rosae(coleseed sawfly) -> XP_012256328.1 Athalia rosae mRNA cap guanine-N7 methyltransferase (LOC105686253), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAt100637
Clone ID Related Accession (Same CDS sequence)	XM_012400905.2
Accession Version	XM_012400905.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1194bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-16
Organism	Athalia rosae(coleseed sawfly)
Product	mRNA cap guanine-N7 methyltransferase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020311966.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 20, 2017 this sequence version replaced XM_012400905.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Athalia rosae Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTTC GAAAAATTTG GTTACTTCAG TATCGTACAA ATAGTGTAGT TAAGGATACC 
AGTGCTCCGT CTGACCTTTC AACAGGCAGC AATAGGGGAG ACGAATATGT TCCCGACAGA 
CAGAAGCCTA CCAGCAACTC TAATGTTGTA GCCGAGCACT ACAACATGCT CGAAGAAAAA 
GGTCTTGGTC AGAGATCTCA AAGCCGCATA CTTTACATGA GAAATTTCAA CAACTGGGTT 
AAAAGCATGC TTATTGGTGA GTACATAGGC AAAGCGCGTC AAGGGAAAGG ACACAGGGTT 
CCATTTAAAG TCCTTGACAT GTGTTGTGGA AAAGGCGGAG ATCTTTTGAA ATGGTCAAAG 
GCTAATATCT CGCATCTTAT TTGCGCTGAT ATAGCAGAAG TTTCGATCCA ACAATGCCAG 
AGCCGCTACA ATGACTTATT GAACAGGAGT TCCAACAACA GAGGATATGC TCCAGCATTT 
ACTGCGGAGT TTATCACGGC AGACTGCACC AAGACAAGAC TGAGAGAAAA ATATAAGGAT 
GCTAGCTCTC AGCTGGATTT GGTAAGCTGC CAATTCGCGT TTCACTACAG TTTTGAGTCG 
TTGCCACAGG CGGAGTGCAT GCTCAAAAAC GCAGGGGAGA GCTTGAGACC CGGAGCGTAT 
TTTATCGGCA CCATTCCTGA TGCTTATGAG CTAGTGTCTC GATGGCAGAA AAGCAACTCT 
GATAAGATTG GAAATGACGT TTACAACATA GAATTTTTAT GCAAAGACAA AGTAAAACCA 
CCGTTGTTCG GGGCCAAATA CAATTTTTAT TTGGAGGGAG TGGTCAACTG TCCGGAATAT 
CTCGTGCATT TGCCAACTCT CACAAAACTT GCGTTGAAGT ATGGACTCGA GCTGGTCATG 
TTTGAAAGGT TTGAAAATTT CTTCAAACGC GTAAAGGACG AGGGGAGATC CCTGCTCGGT 
AAAGTTCAGG CACTCGAGAC ATATCCACCG TGTCACGAGG TGCCACTACT TGGTCAGAAT 
CCCGATGACT ATCGCCACGC TTTGCAGTAC ATGCAGAATT CGAGCGGACA CAGAAAAATT 
GGTACTCTCT CACTACCCGA GTGGGAGGCG ACCTCACTGT ATGCAGTATT CGCTTTTCAA 
AAAATGAAGA CAACGTGGAA TGCCGAAGGA AAACCAGAGT ATGTTAAACT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012256328.1
CDS	75..1268
Translation	MAFRKIWLLQYRTNSVVKDTSAPSDLSTGSNRGDEYVPDRQKPTSNSNVVAEHYNMLEEKGLGQRSQSRILYMRNFNNWVKSMLIGEYIGKARQGKGHRVPFKVLDMCCGKGGDLLKWSKANISHLICADIAEVSIQQCQSRYNDLLNRSSNNRGYAPAFTAEFITADCTKTRLREKYKDASSQLDLVSCQFAFHYSFESLPQAECMLKNAGESLRPGAYFIGTIPDAYELVSRWQKSNSDKIGNDVYNIEFLCKDKVKPPLFGAKYNFYLEGVVNCPEYLVHLPTLTKLALKYGLELVMFERFENFFKRVKDEGRSLLGKVQALETYPPCHEVPLLGQNPDDYRHALQYMQNSSGHRKIGTLSLPEWEATSLYAVFAFQKMKTTWNAEGKPEYVKL

Target ORF information:

RefSeq Version	XM_012400905.2
Organism	Athalia rosae(coleseed sawfly)
Definition	Athalia rosae mRNA cap guanine-N7 methyltransferase (LOC105686253), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012400905.2

ORF Insert Sequence:

ATGGCATTTC GAAAAATTTG GTTACTTCAG TATCGTACAA ATAGTGTAGT TAAGGATACC 
AGTGCTCCGT CTGACCTTTC AACAGGCAGC AATAGGGGAG ACGAATATGT TCCCGACAGA 
CAGAAGCCTA CCAGCAACTC TAATGTTGTA GCCGAGCACT ACAACATGCT CGAAGAAAAA 
GGTCTTGGTC AGAGATCTCA AAGCCGCATA CTTTACATGA GAAATTTCAA CAACTGGGTT 
AAAAGCATGC TTATTGGTGA GTACATAGGC AAAGCGCGTC AAGGGAAAGG ACACAGGGTT 
CCATTTAAAG TCCTTGACAT GTGTTGTGGA AAAGGCGGAG ATCTTTTGAA ATGGTCAAAG 
GCTAATATCT CGCATCTTAT TTGCGCTGAT ATAGCAGAAG TTTCGATCCA ACAATGCCAG 
AGCCGCTACA ATGACTTATT GAACAGGAGT TCCAACAACA GAGGATATGC TCCAGCATTT 
ACTGCGGAGT TTATCACGGC AGACTGCACC AAGACAAGAC TGAGAGAAAA ATATAAGGAT 
GCTAGCTCTC AGCTGGATTT GGTAAGCTGC CAATTCGCGT TTCACTACAG TTTTGAGTCG 
TTGCCACAGG CGGAGTGCAT GCTCAAAAAC GCAGGGGAGA GCTTGAGACC CGGAGCGTAT 
TTTATCGGCA CCATTCCTGA TGCTTATGAG CTAGTGTCTC GATGGCAGAA AAGCAACTCT 
GATAAGATTG GAAATGACGT TTACAACATA GAATTTTTAT GCAAAGACAA AGTAAAACCA 
CCGTTGTTCG GGGCCAAATA CAATTTTTAT TTGGAGGGAG TGGTCAACTG TCCGGAATAT 
CTCGTGCATT TGCCAACTCT CACAAAACTT GCGTTGAAGT ATGGACTCGA GCTGGTCATG 
TTTGAAAGGT TTGAAAATTT CTTCAAACGC GTAAAGGACG AGGGGAGATC CCTGCTCGGT 
AAAGTTCAGG CACTCGAGAC ATATCCACCG TGTCACGAGG TGCCACTACT TGGTCAGAAT 
CCCGATGACT ATCGCCACGC TTTGCAGTAC ATGCAGAATT CGAGCGGACA CAGAAAAATT 
GGTACTCTCT CACTACCCGA GTGGGAGGCG ACCTCACTGT ATGCAGTATT CGCTTTTCAA 
AAAATGAAGA CAACGTGGAA TGCCGAAGGA AAACCAGAGT ATGTTAAACT ATAG