RASGRF1 cDNA ORF clone, Aquila chrysaetos canadensis

Gene
Clones
gRNAs

Summary

Gene Symbol	RASGRF1
Entrez Gene ID	105412609
Full Name	Ras protein specific guanine nucleotide releasing factor 1
Gene Type	protein-coding
Organism	Aquila chrysaetos canadensis

mRNA and Protein(s)

mRNA	Protein	Name
XM_011596521.1	XP_011594823.1	ras-specific guanine nucleotide-releasing factor 1 isoform X2
XM_011596514.1	XP_011594816.1	ras-specific guanine nucleotide-releasing factor 1 isoform X1

Pathways from BioSystems
Gene Ontology

The following RASGRF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RASGRF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAq10960	XM_011596514.1 Latest version!	Aquila chrysaetos canadensis Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OAq10961	XM_011596521.1 Latest version!	Aquila chrysaetos canadensis Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RASGRF1 ( XM_011596514.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011594816.1 Aquila chrysaetos canadensis Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAq10960
Clone ID Related Accession (Same CDS sequence)	XM_011596514.1
Accession Version	XM_011596514.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	ras-specific guanine nucleotide-releasing factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011950872.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGG GGATGCGGCT CAATGACGGG CACGTCACCT ACCTGGGGCT ACTGGCGAAG 
AAGGACGGCG CCAGGAAGGG CTACCTGAGC AAGCGGAGCT CCGACAACAC AAAATGGCAC 
ACCAAGTGGT TCGCGCTGCT GCAGAACATG CTCTTCTACT TCGAGACCGA CTCCAGCTCC 
CGGCCCTCGG GGCTCTACCT GCTCGAGGGC TGCGTCTGCG ACCGGGCGCC CTCCCCCAAG 
CCTTCCCTCT CGGCCAAGGA CTCCCTGGAG AAGCAGCATT ACTTTACCGT TAATTTCAAC 
CACGAGAACC AGAAGACCCT GGAGCTGAGG ACCGAGGATG CCAAGGACTG CGACGAGTGG 
GTGGCAGCCA TAGCTCATGC CAGCTACAGG AACCTGGCGA CGGAGCACGA GGCACTGATG 
CAGAAGTACC TCCATCTCCT GCAGATTGTG GAAACGGAGA AGACTGTTGC CAAGCAACTC 
CGCCAGCAGA TCGAGGACGG GGAGATCGAG ATCGAGCGCT TGAAGGCCGA GATCGCCTCC 
CTACTCAAGG ACCACGAGCG CATCCAGGCC GGCCAGACCA GCGCGCCCAG CGATGATGAC 
AGTGACATCA AGAAAATCAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGAGG 
AAGTGGAAGA CGATCATCCA GGACTACATC AGGTCCCCCC ACGCTGACAG CATGCGCAAG 
AGGAACCAGG TCGTCTTCAG CATGCTGGAG GCAGAGGCCG AGTACGTCCA GCAGCTCCAC 
ATCCTCGTCA ACAACTTCCT GCGGCCGCTG CGGATGGCAG CCAGCTCCAA GAAGCCTCCC 
ATCACGCACG ACGATGTCAG CAGCATATTC CTCAACAGCG AAACAATCAT GTTTTTGCAC 
CAAATCTTTT ACCAAGGCCT GAAGGCAAGG ATATCCAGCT GGCCGACGCT CGTGCTGGCT 
GACTTGTTCG ACATCCTGCT GCCCATGCTG AACATCTACC AGGAGTTTGT GAGGAACCAC 
CAGTACAGCC TGCAGATCCT GGCACACTGC AAGCAGAACC GGGACTTTGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACCC TGGAGACCTT CCTGACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACACTGCACG AACTGCTGGC TCACACCCCC 
CACGAGCATG TGGAGAGGAA CAGCCTGGAT TACGCCAAGT CCAAGCTGGA AGAGCTGTCC 
AGGATAATGC ATGATGAAGT GAGTGAGACC GAAAATATTC GAAAAAATCT GGCGATAGAG 
AGGATGATTA TCGAAGGATG TGAAATTCTG CTGGACACCA GCCAGACTTT TGTAAGACAA 
GGGTCCCTCA TCCAAGTGCC GATGTCGGAG AAAGGGAAGA TCACGCGGGG GCGGCTGGGC 
TCCCTCTCGC TGAAGAAGGA GGGCGAGCGG CAGTGCTTCC TCTTCTCCAA GCACTTGATC 
ATCTGCACCA GGGGCTCCGG AGGCAAGCTG CATCTGACCA AGAACGGCGT GATCTCCCTC 
ATTGACTGCA CGCTGGTAGA GGAGCAGGAG AGCACCGACG AGGACGCAAA AGCATCAGGG 
CAAGACATTG ACCATCTTGA CTTCAAAATT GTGGTGGAGC CAAAAGATTC CTCATCTTTT 
ACGGTTATCC TCGTGGCCTC GTCCAGGCAG GAGAAGGCTG CGTGGACCAG TGACATCAGC 
CAGTGCGTGG ACAACATCCG CTGCAACGGC CTTATGATGA ACGCCTTCGA GGAGAACTCC 
AAGGTCACCG TCCCCCAGAT GATCAAGTCT GATGCCAGCT TGTATTGTGA TGATGTTGAC 
ATTAGGTTCA GTAAAACGAT GAATTCCTGC AAGGTCCTGC AGATCCGCTA TGCAAGCGTG 
GAACGGCTCC TGGAGAGGCT GACGGACCTG CGGTTCCTGA GCATTGACTT TCTGAACACG 
TTCCTGCATT CGTACCGCGT CTTCACCACT GCCCTCGTCG TCCTCGACAA GCTCATCACG 
ATCTACAAGA AGCCGATCAG TGCCATCCCT GCACGGTCCC TGGAGCTCCT GTTTGCAAAC 
AGCCAGAACA ACAAGCTGCT GTACGGCGAG CCCCCCAAGT CCCCCAGAGC CAACCGCAAG 
TTCTCCTCGC CTCCCCCTCT CTCCATCACC AAGTCCTCGT CCCCCAGCCG GCGAAGGAAG 
CTCTCCCTCA ACATCCCCAT CATCACAGGA GGGAAGGCAC TGGACCTGGC TGCTCTCAGC 
TGCTCCTCCA ACGGCTATGC AAGCATGTAC TCCTCCATGG CTCCCTTCAG TAAGACCACC 
CTGGACATAA ACAAACTGTA CGTGTCCAGT AACTATCCCA ATAAAATCCC TGATGAAGGA 
GAAGCAGCCT CAGAAAAGCA AGAGGAGTCA CTGCCAAGCA AGCAAAGCTC AGAGGTATCT 
GTAAGGGAAG AGTCGGACAC AGATCCCAAC CAGAGCGATG AAGCAGAAGC TGAAACTTCC 
CCAACAAAAT CTCCGACAAC TCCTAAGTCC ATCAAGTGCA AAAATTCATC AGATTTCTCA 
TTGTTTTCTT ACAACAATGG CGTGGTCATG ACATCCTGCC GGGAGCTCGA CAGCACCCGC 
AGCGCCCTGT CTGCTGCCTC TGCGTTTGCC ATTGCCACGG CGGGAGCCAA CGAGGGGACC 
CCCACCAAGG AGAAGTACCG CAGGATGTCC CTCGCCAGTG CAGTTTCAGG CTTTCCGACG 
GACCAGCGGA ATGGAGACAA GGAGTTCGTG ATCCGGAGAG CGGCCACCAA CCGGGTCCTC 
AATGTGCTGC GGCACTGGGT CTCCAAACAC TCGCAGGACT TTGAAACCAA TGAGGAGCTG 
AAGTTCAGAG TGATTGCCTT TCTGGAGGAG GTTATTCATG ATCCCGAGCT CCTGACCCAG 
GAGAGGAAAG CAGCCGCCAA CATCATAAGG ACCTTGACGC AGGAGGATCC AGGAGACAAT 
CAGATAACCC TGGAGGAGGT TGTACAGATG GCTGAGGGAG TCAAAGCAGA ACCTTTTGAA 
AACCACTCGG CCCTGGAAAT AGCAGAACAG CTGACGCTGC TGGATCACCT GGTCTTCAAG 
AAGATCCCGT ATGAAGAGTT CTTTGGGCAA GGTTGGATGA AGCTTGAGAA GAACGAAAGG 
ACTCCTTACA TCATGAAGAA CACAAAACAC TTTAATGATG TCAGTAACTT GATAGCGTCG 
GAGATCCTCC GGAACGAGGA GATCAACGCA CGGGTGAGTG CCATCGAGAA GTGGGTGGCG 
GTGGCGGACA TCTGCCGGTG CCTGCACAAC TACAACGCGG TGCTGGAGAT CACCTCCTCC 
CTCAACCGCA GTGCCATCTT CCGTCTGAAG AAAACCTGGC TCAAGGTCTC CAAGCAGACA 
AAGGCTCTGA TCGATAAGCT ACAAAAGCTG GTGTCGTCGG AGGGCAGGTT CAAGAACCTG 
AGGGAGGCAC TGAAAAATTG TGACCCTCCG TGTGTCCCCT ACCTGGGAAT GTACCTGACC 
GACCTGGCAT TCATCGAGGA GGGGACGCCA AATTACACTG AGGATGGCCT GGTGAACTTC 
TCCAAAATGA GGATGATTTC ACACATCATA AGAGAGATCC GCCAGTTCCA GCAAACCTCC 
TACAAGATTG AGCACCAGCC TAAGGTGACG CAGTACCTGC TGGACCAGTC GTTTGTGATG 
GATGAAGAAA CCCTTTACGA AGCTTCTCTG CGAATAGAGC CCAAACTGCC TTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011594816.1
CDS	180..3956
Translation	MQKGMRLNDGHVTYLGLLAKKDGARKGYLSKRSSDNTKWHTKWFALLQNMLFYFETDSSSRPSGLYLLEGCVCDRAPSPKPSLSAKDSLEKQHYFTVNFNHENQKTLELRTEDAKDCDEWVAAIAHASYRNLATEHEALMQKYLHLLQIVETEKTVAKQLRQQIEDGEIEIERLKAEIASLLKDHERIQAGQTSAPSDDDSDIKKIKKVQSFLRGWLCRRKWKTIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKHYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMIIEGCEILLDTSQTFVRQGSLIQVPMSEKGKITRGRLGSLSLKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLVEEQESTDEDAKASGQDIDHLDFKIVVEPKDSSSFTVILVASSRQEKAAWTSDISQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTALVVLDKLITIYKKPISAIPARSLELLFANSQNNKLLYGEPPKSPRANRKFSSPPPLSITKSSSPSRRRKLSLNIPIITGGKALDLAALSCSSNGYASMYSSMAPFSKTTLDINKLYVSSNYPNKIPDEGEAASEKQEESLPSKQSSEVSVREESDTDPNQSDEAEAETSPTKSPTTPKSIKCKNSSDFSLFSYNNGVVMTSCRELDSTRSALSAASAFAIATAGANEGTPTKEKYRRMSLASAVSGFPTDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNEELKFRVIAFLEEVIHDPELLTQERKAAANIIRTLTQEDPGDNQITLEEVVQMAEGVKAEPFENHSALEIAEQLTLLDHLVFKKIPYEEFFGQGWMKLEKNERTPYIMKNTKHFNDVSNLIASEILRNEEINARVSAIEKWVAVADICRCLHNYNAVLEITSSLNRSAIFRLKKTWLKVSKQTKALIDKLQKLVSSEGRFKNLREALKNCDPPCVPYLGMYLTDLAFIEEGTPNYTEDGLVNFSKMRMISHIIREIRQFQQTSYKIEHQPKVTQYLLDQSFVMDEETLYEASLRIEPKLPS

Target ORF information:

RefSeq Version	XM_011596514.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011596514.1

ORF Insert Sequence:

ATGCAGAAGG GGATGCGGCT CAATGACGGG CACGTCACCT ACCTGGGGCT ACTGGCGAAG 
AAGGACGGCG CCAGGAAGGG CTACCTGAGC AAGCGGAGCT CCGACAACAC AAAATGGCAC 
ACCAAGTGGT TCGCGCTGCT GCAGAACATG CTCTTCTACT TCGAGACCGA CTCCAGCTCC 
CGGCCCTCGG GGCTCTACCT GCTCGAGGGC TGCGTCTGCG ACCGGGCGCC CTCCCCCAAG 
CCTTCCCTCT CGGCCAAGGA CTCCCTGGAG AAGCAGCATT ACTTTACCGT TAATTTCAAC 
CACGAGAACC AGAAGACCCT GGAGCTGAGG ACCGAGGATG CCAAGGACTG CGACGAGTGG 
GTGGCAGCCA TAGCTCATGC CAGCTACAGG AACCTGGCGA CGGAGCACGA GGCACTGATG 
CAGAAGTACC TCCATCTCCT GCAGATTGTG GAAACGGAGA AGACTGTTGC CAAGCAACTC 
CGCCAGCAGA TCGAGGACGG GGAGATCGAG ATCGAGCGCT TGAAGGCCGA GATCGCCTCC 
CTACTCAAGG ACCACGAGCG CATCCAGGCC GGCCAGACCA GCGCGCCCAG CGATGATGAC 
AGTGACATCA AGAAAATCAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGAGG 
AAGTGGAAGA CGATCATCCA GGACTACATC AGGTCCCCCC ACGCTGACAG CATGCGCAAG 
AGGAACCAGG TCGTCTTCAG CATGCTGGAG GCAGAGGCCG AGTACGTCCA GCAGCTCCAC 
ATCCTCGTCA ACAACTTCCT GCGGCCGCTG CGGATGGCAG CCAGCTCCAA GAAGCCTCCC 
ATCACGCACG ACGATGTCAG CAGCATATTC CTCAACAGCG AAACAATCAT GTTTTTGCAC 
CAAATCTTTT ACCAAGGCCT GAAGGCAAGG ATATCCAGCT GGCCGACGCT CGTGCTGGCT 
GACTTGTTCG ACATCCTGCT GCCCATGCTG AACATCTACC AGGAGTTTGT GAGGAACCAC 
CAGTACAGCC TGCAGATCCT GGCACACTGC AAGCAGAACC GGGACTTTGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACCC TGGAGACCTT CCTGACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACACTGCACG AACTGCTGGC TCACACCCCC 
CACGAGCATG TGGAGAGGAA CAGCCTGGAT TACGCCAAGT CCAAGCTGGA AGAGCTGTCC 
AGGATAATGC ATGATGAAGT GAGTGAGACC GAAAATATTC GAAAAAATCT GGCGATAGAG 
AGGATGATTA TCGAAGGATG TGAAATTCTG CTGGACACCA GCCAGACTTT TGTAAGACAA 
GGGTCCCTCA TCCAAGTGCC GATGTCGGAG AAAGGGAAGA TCACGCGGGG GCGGCTGGGC 
TCCCTCTCGC TGAAGAAGGA GGGCGAGCGG CAGTGCTTCC TCTTCTCCAA GCACTTGATC 
ATCTGCACCA GGGGCTCCGG AGGCAAGCTG CATCTGACCA AGAACGGCGT GATCTCCCTC 
ATTGACTGCA CGCTGGTAGA GGAGCAGGAG AGCACCGACG AGGACGCAAA AGCATCAGGG 
CAAGACATTG ACCATCTTGA CTTCAAAATT GTGGTGGAGC CAAAAGATTC CTCATCTTTT 
ACGGTTATCC TCGTGGCCTC GTCCAGGCAG GAGAAGGCTG CGTGGACCAG TGACATCAGC 
CAGTGCGTGG ACAACATCCG CTGCAACGGC CTTATGATGA ACGCCTTCGA GGAGAACTCC 
AAGGTCACCG TCCCCCAGAT GATCAAGTCT GATGCCAGCT TGTATTGTGA TGATGTTGAC 
ATTAGGTTCA GTAAAACGAT GAATTCCTGC AAGGTCCTGC AGATCCGCTA TGCAAGCGTG 
GAACGGCTCC TGGAGAGGCT GACGGACCTG CGGTTCCTGA GCATTGACTT TCTGAACACG 
TTCCTGCATT CGTACCGCGT CTTCACCACT GCCCTCGTCG TCCTCGACAA GCTCATCACG 
ATCTACAAGA AGCCGATCAG TGCCATCCCT GCACGGTCCC TGGAGCTCCT GTTTGCAAAC 
AGCCAGAACA ACAAGCTGCT GTACGGCGAG CCCCCCAAGT CCCCCAGAGC CAACCGCAAG 
TTCTCCTCGC CTCCCCCTCT CTCCATCACC AAGTCCTCGT CCCCCAGCCG GCGAAGGAAG 
CTCTCCCTCA ACATCCCCAT CATCACAGGA GGGAAGGCAC TGGACCTGGC TGCTCTCAGC 
TGCTCCTCCA ACGGCTATGC AAGCATGTAC TCCTCCATGG CTCCCTTCAG TAAGACCACC 
CTGGACATAA ACAAACTGTA CGTGTCCAGT AACTATCCCA ATAAAATCCC TGATGAAGGA 
GAAGCAGCCT CAGAAAAGCA AGAGGAGTCA CTGCCAAGCA AGCAAAGCTC AGAGGTATCT 
GTAAGGGAAG AGTCGGACAC AGATCCCAAC CAGAGCGATG AAGCAGAAGC TGAAACTTCC 
CCAACAAAAT CTCCGACAAC TCCTAAGTCC ATCAAGTGCA AAAATTCATC AGATTTCTCA 
TTGTTTTCTT ACAACAATGG CGTGGTCATG ACATCCTGCC GGGAGCTCGA CAGCACCCGC 
AGCGCCCTGT CTGCTGCCTC TGCGTTTGCC ATTGCCACGG CGGGAGCCAA CGAGGGGACC 
CCCACCAAGG AGAAGTACCG CAGGATGTCC CTCGCCAGTG CAGTTTCAGG CTTTCCGACG 
GACCAGCGGA ATGGAGACAA GGAGTTCGTG ATCCGGAGAG CGGCCACCAA CCGGGTCCTC 
AATGTGCTGC GGCACTGGGT CTCCAAACAC TCGCAGGACT TTGAAACCAA TGAGGAGCTG 
AAGTTCAGAG TGATTGCCTT TCTGGAGGAG GTTATTCATG ATCCCGAGCT CCTGACCCAG 
GAGAGGAAAG CAGCCGCCAA CATCATAAGG ACCTTGACGC AGGAGGATCC AGGAGACAAT 
CAGATAACCC TGGAGGAGGT TGTACAGATG GCTGAGGGAG TCAAAGCAGA ACCTTTTGAA 
AACCACTCGG CCCTGGAAAT AGCAGAACAG CTGACGCTGC TGGATCACCT GGTCTTCAAG 
AAGATCCCGT ATGAAGAGTT CTTTGGGCAA GGTTGGATGA AGCTTGAGAA GAACGAAAGG 
ACTCCTTACA TCATGAAGAA CACAAAACAC TTTAATGATG TCAGTAACTT GATAGCGTCG 
GAGATCCTCC GGAACGAGGA GATCAACGCA CGGGTGAGTG CCATCGAGAA GTGGGTGGCG 
GTGGCGGACA TCTGCCGGTG CCTGCACAAC TACAACGCGG TGCTGGAGAT CACCTCCTCC 
CTCAACCGCA GTGCCATCTT CCGTCTGAAG AAAACCTGGC TCAAGGTCTC CAAGCAGACA 
AAGGCTCTGA TCGATAAGCT ACAAAAGCTG GTGTCGTCGG AGGGCAGGTT CAAGAACCTG 
AGGGAGGCAC TGAAAAATTG TGACCCTCCG TGTGTCCCCT ACCTGGGAAT GTACCTGACC 
GACCTGGCAT TCATCGAGGA GGGGACGCCA AATTACACTG AGGATGGCCT GGTGAACTTC 
TCCAAAATGA GGATGATTTC ACACATCATA AGAGAGATCC GCCAGTTCCA GCAAACCTCC 
TACAAGATTG AGCACCAGCC TAAGGTGACG CAGTACCTGC TGGACCAGTC GTTTGTGATG 
GATGAAGAAA CCCTTTACGA AGCTTCTCTG CGAATAGAGC CCAAACTGCC TTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRF1 ( XM_011596521.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011594823.1 Aquila chrysaetos canadensis Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAq10961
Clone ID Related Accession (Same CDS sequence)	XM_011596521.1
Accession Version	XM_011596521.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3738bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	ras-specific guanine nucleotide-releasing factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011950872.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGG GGATGCGGCT CAATGACGGG CACGTCACCT ACCTGGGGCT ACTGGCGAAG 
AAGGACGGCG CCAGGAAGGG CTACCTGAGC AAGCGGAGCT CCGACAACAC AAAATGGCAC 
ACCAAGTGGT TCGCGCTGCT GCAGAACATG CTCTTCTACT TCGAGACCGA CTCCAGCTCC 
CGGCCCTCGG GGCTCTACCT GCTCGAGGGC TGCGTCTGCG ACCGGGCGCC CTCCCCCAAG 
CCTTCCCTCT CGGCCAAGGA CTCCCTGGAG AAGCAGCATT ACTTTACCGT TAATTTCAAC 
CACGAGAACC AGAAGACCCT GGAGCTGAGG ACCGAGGATG CCAAGGACTG CGACGAGTGG 
GTGGCAGCCA TAGCTCATGC CAGCTACAGG AACCTGGCGA CGGAGCACGA GGCACTGATG 
CAGAAGTACC TCCATCTCCT GCAGATTGTG GAAACGGAGA AGACTGTTGC CAAGCAACTC 
CGCCAGCAGA TCGAGGACGG GGAGATCGAG ATCGAGCGCT TGAAGGCCGA GATCGCCTCC 
CTACTCAAGG ACCACGAGCG CATCCAGGCC GGCCAGACCA GCGCGCCCAG CGATGATGAC 
AGTGACATCA AGAAAATCAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGAGG 
AAGTGGAAGA CGATCATCCA GGACTACATC AGGTCCCCCC ACGCTGACAG CATGCGCAAG 
AGGAACCAGG TCGTCTTCAG CATGCTGGAG GCAGAGGCCG AGTACGTCCA GCAGCTCCAC 
ATCCTCGTCA ACAACTTCCT GCGGCCGCTG CGGATGGCAG CCAGCTCCAA GAAGCCTCCC 
ATCACGCACG ACGATGTCAG CAGCATATTC CTCAACAGCG AAACAATCAT GTTTTTGCAC 
CAAATCTTTT ACCAAGGCCT GAAGGCAAGG ATATCCAGCT GGCCGACGCT CGTGCTGGCT 
GACTTGTTCG ACATCCTGCT GCCCATGCTG AACATCTACC AGGAGTTTGT GAGGAACCAC 
CAGTACAGCC TGCAGATCCT GGCACACTGC AAGCAGAACC GGGACTTTGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACCC TGGAGACCTT CCTGACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACACTGCACG AACTGCTGGC TCACACCCCC 
CACGAGCATG TGGAGAGGAA CAGCCTGGAT TACGCCAAGT CCAAGCTGGA AGAGCTGTCC 
AGGATAATGC ATGATGAAGT GAGTGAGACC GAAAATATTC GAAAAAATCT GGCGATAGAG 
AGGATGATTA TCGAAGGATG TGAAATTCTG CTGGACACCA GCCAGACTTT TGTAAGACAA 
GGGTCCCTCA TCCAAGTGCC GATGTCGGAG AAAGGGAAGA TCACGCGGGG GCGGCTGGGC 
TCCCTCTCGC TGAAGAAGGA GGGCGAGCGG CAGTGCTTCC TCTTCTCCAA GCACTTGATC 
ATCTGCACCA GGGGCTCCGG AGGCAAGCTG CATCTGACCA AGAACGGCGT GATCTCCCTC 
ATTGACTGCA CGCTGGTAGA GGAGCAGGAG AGCACCGACG AGGACGCAAA AGCATCAGGG 
CAAGACATTG ACCATCTTGA CTTCAAAATT GTGGTGGAGC CAAAAGATTC CTCATCTTTT 
ACGGTTATCC TCGTGGCCTC GTCCAGGCAG GAGAAGGCTG CGTGGACCAG TGACATCAGC 
CAGTGCGTGG ACAACATCCG CTGCAACGGC CTTATGATGA ACGCCTTCGA GGAGAACTCC 
AAGGTCACCG TCCCCCAGAT GATCAAGTTC AGTAAAACGA TGAATTCCTG CAAGGTCCTG 
CAGATCCGCT ATGCAAGCGT GGAACGGCTC CTGGAGAGGC TGACGGACCT GCGGTTCCTG 
AGCATTGACT TTCTGAACAC GTTCCTGCAT TCGTACCGCG TCTTCACCAC TGCCCTCGTC 
GTCCTCGACA AGCTCATCAC GATCTACAAG AAGCCGATCA GTGCCATCCC TGCACGGTCC 
CTGGAGCTCC TGTTTGCAAA CAGCCAGAAC AACAAGCTGC TGTACGGCGA GCCCCCCAAG 
TCCCCCAGAG CCAACCGCAA GTTCTCCTCG CCTCCCCCTC TCTCCATCAC CAAGTCCTCG 
TCCCCCAGCC GGCGAAGGAA GCTCTCCCTC AACATCCCCA TCATCACAGG AGGGAAGGCA 
CTGGACCTGG CTGCTCTCAG CTGCTCCTCC AACGGCTATG CAAGCATGTA CTCCTCCATG 
GCTCCCTTCA GTAAGACCAC CCTGGACATA AACAAACTGT ACGTGTCCAG TAACTATCCC 
AATAAAATCC CTGATGAAGG AGAAGCAGCC TCAGAAAAGC AAGAGGAGTC ACTGCCAAGC 
AAGCAAAGCT CAGAGGTATC TGTAAGGGAA GAGTCGGACA CAGATCCCAA CCAGAGCGAT 
GAAGCAGAAG CTGAAACTTC CCCAACAAAA TCTCCGACAA CTCCTAAGTC CATCAAGTGC 
AAAAATTCAT CAGATTTCTC ATTGTTTTCT TACAACAATG GCGTGGTCAT GACATCCTGC 
CGGGAGCTCG ACAGCACCCG CAGCGCCCTG TCTGCTGCCT CTGCGTTTGC CATTGCCACG 
GCGGGAGCCA ACGAGGGGAC CCCCACCAAG GAGAAGTACC GCAGGATGTC CCTCGCCAGT 
GCAGTTTCAG GCTTTCCGAC GGACCAGCGG AATGGAGACA AGGAGTTCGT GATCCGGAGA 
GCGGCCACCA ACCGGGTCCT CAATGTGCTG CGGCACTGGG TCTCCAAACA CTCGCAGGAC 
TTTGAAACCA ATGAGGAGCT GAAGTTCAGA GTGATTGCCT TTCTGGAGGA GGTTATTCAT 
GATCCCGAGC TCCTGACCCA GGAGAGGAAA GCAGCCGCCA ACATCATAAG GACCTTGACG 
CAGGAGGATC CAGGAGACAA TCAGATAACC CTGGAGGAGG TTGTACAGAT GGCTGAGGGA 
GTCAAAGCAG AACCTTTTGA AAACCACTCG GCCCTGGAAA TAGCAGAACA GCTGACGCTG 
CTGGATCACC TGGTCTTCAA GAAGATCCCG TATGAAGAGT TCTTTGGGCA AGGTTGGATG 
AAGCTTGAGA AGAACGAAAG GACTCCTTAC ATCATGAAGA ACACAAAACA CTTTAATGAT 
GTCAGTAACT TGATAGCGTC GGAGATCCTC CGGAACGAGG AGATCAACGC ACGGGTGAGT 
GCCATCGAGA AGTGGGTGGC GGTGGCGGAC ATCTGCCGGT GCCTGCACAA CTACAACGCG 
GTGCTGGAGA TCACCTCCTC CCTCAACCGC AGTGCCATCT TCCGTCTGAA GAAAACCTGG 
CTCAAGGTCT CCAAGCAGAC AAAGGCTCTG ATCGATAAGC TACAAAAGCT GGTGTCGTCG 
GAGGGCAGGT TCAAGAACCT GAGGGAGGCA CTGAAAAATT GTGACCCTCC GTGTGTCCCC 
TACCTGGGAA TGTACCTGAC CGACCTGGCA TTCATCGAGG AGGGGACGCC AAATTACACT 
GAGGATGGCC TGGTGAACTT CTCCAAAATG AGGATGATTT CACACATCAT AAGAGAGATC 
CGCCAGTTCC AGCAAACCTC CTACAAGATT GAGCACCAGC CTAAGGTGAC GCAGTACCTG 
CTGGACCAGT CGTTTGTGAT GGATGAAGAA ACCCTTTACG AAGCTTCTCT GCGAATAGAG 
CCCAAACTGC CTTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011594823.1
CDS	180..3917
Translation	MQKGMRLNDGHVTYLGLLAKKDGARKGYLSKRSSDNTKWHTKWFALLQNMLFYFETDSSSRPSGLYLLEGCVCDRAPSPKPSLSAKDSLEKQHYFTVNFNHENQKTLELRTEDAKDCDEWVAAIAHASYRNLATEHEALMQKYLHLLQIVETEKTVAKQLRQQIEDGEIEIERLKAEIASLLKDHERIQAGQTSAPSDDDSDIKKIKKVQSFLRGWLCRRKWKTIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKHYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMIIEGCEILLDTSQTFVRQGSLIQVPMSEKGKITRGRLGSLSLKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLVEEQESTDEDAKASGQDIDHLDFKIVVEPKDSSSFTVILVASSRQEKAAWTSDISQCVDNIRCNGLMMNAFEENSKVTVPQMIKFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTALVVLDKLITIYKKPISAIPARSLELLFANSQNNKLLYGEPPKSPRANRKFSSPPPLSITKSSSPSRRRKLSLNIPIITGGKALDLAALSCSSNGYASMYSSMAPFSKTTLDINKLYVSSNYPNKIPDEGEAASEKQEESLPSKQSSEVSVREESDTDPNQSDEAEAETSPTKSPTTPKSIKCKNSSDFSLFSYNNGVVMTSCRELDSTRSALSAASAFAIATAGANEGTPTKEKYRRMSLASAVSGFPTDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNEELKFRVIAFLEEVIHDPELLTQERKAAANIIRTLTQEDPGDNQITLEEVVQMAEGVKAEPFENHSALEIAEQLTLLDHLVFKKIPYEEFFGQGWMKLEKNERTPYIMKNTKHFNDVSNLIASEILRNEEINARVSAIEKWVAVADICRCLHNYNAVLEITSSLNRSAIFRLKKTWLKVSKQTKALIDKLQKLVSSEGRFKNLREALKNCDPPCVPYLGMYLTDLAFIEEGTPNYTEDGLVNFSKMRMISHIIREIRQFQQTSYKIEHQPKVTQYLLDQSFVMDEETLYEASLRIEPKLPS

Target ORF information:

RefSeq Version	XM_011596521.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011596521.1

ORF Insert Sequence:

ATGCAGAAGG GGATGCGGCT CAATGACGGG CACGTCACCT ACCTGGGGCT ACTGGCGAAG 
AAGGACGGCG CCAGGAAGGG CTACCTGAGC AAGCGGAGCT CCGACAACAC AAAATGGCAC 
ACCAAGTGGT TCGCGCTGCT GCAGAACATG CTCTTCTACT TCGAGACCGA CTCCAGCTCC 
CGGCCCTCGG GGCTCTACCT GCTCGAGGGC TGCGTCTGCG ACCGGGCGCC CTCCCCCAAG 
CCTTCCCTCT CGGCCAAGGA CTCCCTGGAG AAGCAGCATT ACTTTACCGT TAATTTCAAC 
CACGAGAACC AGAAGACCCT GGAGCTGAGG ACCGAGGATG CCAAGGACTG CGACGAGTGG 
GTGGCAGCCA TAGCTCATGC CAGCTACAGG AACCTGGCGA CGGAGCACGA GGCACTGATG 
CAGAAGTACC TCCATCTCCT GCAGATTGTG GAAACGGAGA AGACTGTTGC CAAGCAACTC 
CGCCAGCAGA TCGAGGACGG GGAGATCGAG ATCGAGCGCT TGAAGGCCGA GATCGCCTCC 
CTACTCAAGG ACCACGAGCG CATCCAGGCC GGCCAGACCA GCGCGCCCAG CGATGATGAC 
AGTGACATCA AGAAAATCAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGAGG 
AAGTGGAAGA CGATCATCCA GGACTACATC AGGTCCCCCC ACGCTGACAG CATGCGCAAG 
AGGAACCAGG TCGTCTTCAG CATGCTGGAG GCAGAGGCCG AGTACGTCCA GCAGCTCCAC 
ATCCTCGTCA ACAACTTCCT GCGGCCGCTG CGGATGGCAG CCAGCTCCAA GAAGCCTCCC 
ATCACGCACG ACGATGTCAG CAGCATATTC CTCAACAGCG AAACAATCAT GTTTTTGCAC 
CAAATCTTTT ACCAAGGCCT GAAGGCAAGG ATATCCAGCT GGCCGACGCT CGTGCTGGCT 
GACTTGTTCG ACATCCTGCT GCCCATGCTG AACATCTACC AGGAGTTTGT GAGGAACCAC 
CAGTACAGCC TGCAGATCCT GGCACACTGC AAGCAGAACC GGGACTTTGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC CGACTGCGAG GAGAGGACCC TGGAGACCTT CCTGACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACACTGCACG AACTGCTGGC TCACACCCCC 
CACGAGCATG TGGAGAGGAA CAGCCTGGAT TACGCCAAGT CCAAGCTGGA AGAGCTGTCC 
AGGATAATGC ATGATGAAGT GAGTGAGACC GAAAATATTC GAAAAAATCT GGCGATAGAG 
AGGATGATTA TCGAAGGATG TGAAATTCTG CTGGACACCA GCCAGACTTT TGTAAGACAA 
GGGTCCCTCA TCCAAGTGCC GATGTCGGAG AAAGGGAAGA TCACGCGGGG GCGGCTGGGC 
TCCCTCTCGC TGAAGAAGGA GGGCGAGCGG CAGTGCTTCC TCTTCTCCAA GCACTTGATC 
ATCTGCACCA GGGGCTCCGG AGGCAAGCTG CATCTGACCA AGAACGGCGT GATCTCCCTC 
ATTGACTGCA CGCTGGTAGA GGAGCAGGAG AGCACCGACG AGGACGCAAA AGCATCAGGG 
CAAGACATTG ACCATCTTGA CTTCAAAATT GTGGTGGAGC CAAAAGATTC CTCATCTTTT 
ACGGTTATCC TCGTGGCCTC GTCCAGGCAG GAGAAGGCTG CGTGGACCAG TGACATCAGC 
CAGTGCGTGG ACAACATCCG CTGCAACGGC CTTATGATGA ACGCCTTCGA GGAGAACTCC 
AAGGTCACCG TCCCCCAGAT GATCAAGTTC AGTAAAACGA TGAATTCCTG CAAGGTCCTG 
CAGATCCGCT ATGCAAGCGT GGAACGGCTC CTGGAGAGGC TGACGGACCT GCGGTTCCTG 
AGCATTGACT TTCTGAACAC GTTCCTGCAT TCGTACCGCG TCTTCACCAC TGCCCTCGTC 
GTCCTCGACA AGCTCATCAC GATCTACAAG AAGCCGATCA GTGCCATCCC TGCACGGTCC 
CTGGAGCTCC TGTTTGCAAA CAGCCAGAAC AACAAGCTGC TGTACGGCGA GCCCCCCAAG 
TCCCCCAGAG CCAACCGCAA GTTCTCCTCG CCTCCCCCTC TCTCCATCAC CAAGTCCTCG 
TCCCCCAGCC GGCGAAGGAA GCTCTCCCTC AACATCCCCA TCATCACAGG AGGGAAGGCA 
CTGGACCTGG CTGCTCTCAG CTGCTCCTCC AACGGCTATG CAAGCATGTA CTCCTCCATG 
GCTCCCTTCA GTAAGACCAC CCTGGACATA AACAAACTGT ACGTGTCCAG TAACTATCCC 
AATAAAATCC CTGATGAAGG AGAAGCAGCC TCAGAAAAGC AAGAGGAGTC ACTGCCAAGC 
AAGCAAAGCT CAGAGGTATC TGTAAGGGAA GAGTCGGACA CAGATCCCAA CCAGAGCGAT 
GAAGCAGAAG CTGAAACTTC CCCAACAAAA TCTCCGACAA CTCCTAAGTC CATCAAGTGC 
AAAAATTCAT CAGATTTCTC ATTGTTTTCT TACAACAATG GCGTGGTCAT GACATCCTGC 
CGGGAGCTCG ACAGCACCCG CAGCGCCCTG TCTGCTGCCT CTGCGTTTGC CATTGCCACG 
GCGGGAGCCA ACGAGGGGAC CCCCACCAAG GAGAAGTACC GCAGGATGTC CCTCGCCAGT 
GCAGTTTCAG GCTTTCCGAC GGACCAGCGG AATGGAGACA AGGAGTTCGT GATCCGGAGA 
GCGGCCACCA ACCGGGTCCT CAATGTGCTG CGGCACTGGG TCTCCAAACA CTCGCAGGAC 
TTTGAAACCA ATGAGGAGCT GAAGTTCAGA GTGATTGCCT TTCTGGAGGA GGTTATTCAT 
GATCCCGAGC TCCTGACCCA GGAGAGGAAA GCAGCCGCCA ACATCATAAG GACCTTGACG 
CAGGAGGATC CAGGAGACAA TCAGATAACC CTGGAGGAGG TTGTACAGAT GGCTGAGGGA 
GTCAAAGCAG AACCTTTTGA AAACCACTCG GCCCTGGAAA TAGCAGAACA GCTGACGCTG 
CTGGATCACC TGGTCTTCAA GAAGATCCCG TATGAAGAGT TCTTTGGGCA AGGTTGGATG 
AAGCTTGAGA AGAACGAAAG GACTCCTTAC ATCATGAAGA ACACAAAACA CTTTAATGAT 
GTCAGTAACT TGATAGCGTC GGAGATCCTC CGGAACGAGG AGATCAACGC ACGGGTGAGT 
GCCATCGAGA AGTGGGTGGC GGTGGCGGAC ATCTGCCGGT GCCTGCACAA CTACAACGCG 
GTGCTGGAGA TCACCTCCTC CCTCAACCGC AGTGCCATCT TCCGTCTGAA GAAAACCTGG 
CTCAAGGTCT CCAAGCAGAC AAAGGCTCTG ATCGATAAGC TACAAAAGCT GGTGTCGTCG 
GAGGGCAGGT TCAAGAACCT GAGGGAGGCA CTGAAAAATT GTGACCCTCC GTGTGTCCCC 
TACCTGGGAA TGTACCTGAC CGACCTGGCA TTCATCGAGG AGGGGACGCC AAATTACACT 
GAGGATGGCC TGGTGAACTT CTCCAAAATG AGGATGATTT CACACATCAT AAGAGAGATC 
CGCCAGTTCC AGCAAACCTC CTACAAGATT GAGCACCAGC CTAAGGTGAC GCAGTACCTG 
CTGGACCAGT CGTTTGTGAT GGATGAAGAA ACCCTTTACG AAGCTTCTCT GCGAATAGAG 
CCCAAACTGC CTTCTTGA