EDEM3 cDNA ORF clone, Aquila chrysaetos canadensis

Gene
Clones
gRNAs

Summary

Gene Symbol	EDEM3
Entrez Gene ID	105408521
Full Name	ER degradation enhancing alpha-mannosidase like protein 3
Gene Type	protein-coding
Organism	Aquila chrysaetos canadensis

mRNA and Protein(s)

mRNA	Protein	Name
XM_011588765.1	XP_011587067.1	ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X1
XM_011588766.1	XP_011587068.1	ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X2

Pathways from BioSystems
Gene Ontology

The following EDEM3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EDEM3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAq24867	XM_011588765.1 Latest version!	Aquila chrysaetos canadensis ER degradation enhancer, mannosidase alpha-like 3 (EDEM3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OAq24868	XM_011588766.1 Latest version!	Aquila chrysaetos canadensis ER degradation enhancer, mannosidase alpha-like 3 (EDEM3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EDEM3 ( XM_011588765.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011587067.1 Aquila chrysaetos canadensis ER degradation enhancer, mannosidase alpha-like 3 (EDEM3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAq24867
Clone ID Related Accession (Same CDS sequence)	XM_011588765.1
Accession Version	XM_011588765.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011950937.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGAG CTGCGGGCTG CCAGGGGGGC GGTGGCGGCG GCGAGCGGGG GCACCGCTGG 
AGGAGGCCGT GGAAGCTCCT GGCCCTTGGC CTGCTCTCCG CCTCCTCCGT GCTGGCGGCC 
GCCCCGGGCG CCGGGGCCAT GAGCAAGGAG GAGAAGCGCC GGTTGGGAAA CCAAGTGCTC 
GAAATGTTTG ATCATGCATA TAGCAATTAT ATGGAGCATG CGTATCCAGC TGATGAACTC 
ATGCCTTTAA CTTGTCGTGG ACGAGTACGG GGTCAGGAAC CAAGTCGAGG GGATGTGGAT 
GATGCCTTGG GAAAATTTTC ACTGACCTTG ATTGATACCT TGGACACTCT TGTGGTGTTA 
AATAAAACCA AAGAGTTTGA AGAGGCTGTA AAAAAAGTAA TAAAGGATGT TAATTTAGAT 
AACGACATAG TTGTATCTGT CTTTGAAACC AACATAAGAG TCCTTGGAGG TCTCCTAGGT 
GGGCATTCAG TAGCAATTAT GCTGAAAGAA AAAGGTGAAT ATATGCAGTG GTACAATGGT 
GAACTTCTGC ACATGGCAAA GGAACTAGGC TACAAGCTTT TACCTGCTTT TAACACCACT 
AGCGGTCTCC CTTATCCAAG AGTTAACTTA AAATTTGGTG TAAGACATCC AGAAGCACGA 
ACAGGAACAG AAACTGATAC CTGTACAGCT TGTGCGGGTA CCTTGATCCT TGAGTTTGCA 
GCTTTAAGCC GATTCACGGG AACATCTATA TTTGAGGAGT ATGCACGAAA AGCGCTTGAT 
TTTATTTGGG AAAAGAGACA ACGCAGCAGC AATTTAGTGG GCGTTACTAT AAACATTCAT 
ACTGGAGACT GGGTCCGCAA AGATAGTGGA GTTGGAGCAG GAATAGATTC ATATTATGAA 
TATTTGTTAA AAGCCTATGT CTTGCTGGGA GATGACAGTT TCCTGGAAAG ATTTAACACG 
CACTACGATG CCATAATGAG ATACATTAGC CAACCACCTC TTCTTCTTGA TGTTCATATT 
CATAAACCAA TGCTAAATGC TAGGACCTGG ATGGATTCTT TGCTAGCTTT CTTCCCTGGA 
TTGCAGGTGC TGAAGGGTGA TATTAGACCT GCTATTGAAA CTCATGAGAT GCTGTATCAG 
GTTATAAAAA AGCATAACTT TCTTCCAGAG GCATTCACAA CAGACTTTAG AGTTCACTGG 
GCTCAGCATC CTTTAAGGCC TGAATTTGCT GAAAGCACTT ACTTCTTGTA TAAAGCTACT 
GGTGACCCTT ACTATCTAGA AGTAGGAAAA ACACTCATTG AAAACTTGAA CAAGTATGCA 
AGAGTACCTT GTGGGTTTGC TGCAATGAAG GATGTTCGTA CAGGAAGTCA TGAAGACAGA 
ATGGACTCTT TCTTTCTGGC TGAGATGTTT AAATATCTTT ATCTGCTGTT TGCTGATAAG 
GAAGACATGA TTTTTGACAT TGAAGATTAT ATCTTTACTA CAGAAGCTCA TCTATTACCA 
CTTTGGCTTT CCACCACAAA CCAAACTATC TCTAAAAAAA ATACAACTAC AGAATACACA 
GAGCTGGATG ACAGCAACTT TGACTGGACC TGTCCAAACA CCCAGATTCT TTTCCCAAAT 
GACCCTATGT TTGCTCAAAG TATTCGTGAA CCTTTGAAAA ATGTGGTGGA TAAGAGCTGT 
CCTAGGGGTA TTTCCAGAGC AGAAGAGAGT TTGGGAAGTG GACCAAAACC ACCACTGAGA 
GCCAGAGATT TCATGGCCAG TAATCCTGAA CACTTGGAGA TACTGAAGAA GATGGGAGTC 
AGTTTGATTC ATCTGAAGGA TGGAAGAGTA CAATTAGTAC AGCATGCAGT GCAAGCAGCA 
AGTTCACTTG ATGCTGAAGA TGGCTTACGG TTCATGCAAG AAATGATTGA ACTCTCCAGT 
CAGCAACAGA AAGAGCAACA GCTACCTCCT CGTGCTGTTC AAATTGTTTC CCATCCATTC 
TTTGGCAGGG TCGTCTTGAC TGCTGGACCA GCACAATTTG GGATGGACTT ATCCAAACAC 
AAAGCAGGGA CAAGAGGATT TGTTGCAACC ATTAAGCCAT ATAATGGATG CTCAGAGATC 
ACTAATCCTG AGGCAGTGAA AGAGAAAATT GCTCTGATGC AAAGAGGCCA GTGTATGTTT 
GCTGAAAAGG CACGAAACAT TCAAAAAGCT GGAGCAATAG GAGGCATTGT TATTGATGAC 
AATGAGGGAA GCAGTAGTGA CACAGCTCCT CTCTTCCAAA TGGCCGGTGA TGGAAAAAAC 
ACAGATGATA TCACAATTCC CATGCTCTTC CTCTTCAACA AAGAAGGAAA CATTATACTG 
GATGCAATCC GGGAGTATGA GGCTGTGGAG GTGCTCCTTT CTGATAAAGC AAAAGACAGA 
GCAACAATCT TTAAAGGTAA AATGATTCCA AACTACATTA TTGATAGCAA CTTAGAAGTG 
GAGAATATGG ACCAGAAATT GTCTGAAAAT GATTCACATA AACAGAATGC AGAAGAAGCT 
ACTTCAGCAT CTCAGGATGT TGGTGCAGTC AGTGAGGAGC CTGAAGAAGG AGAAAGCTCT 
GATGTTACTG ACCCTGATTC TTTATCTCCT GCTAATGCAG ACAGTGACAG CGTTTCCATT 
TCAAATCAGG ATTCCTATGT CCCTGGAACC GATGAGGCTG GTGCTCCGGA GCCAGCATGT 
ACGCGAGGGG ATAACCAGCC TCAGGAACAA AAGACTGAGG CAGAGTCAAA TTCCAAAGTT 
AACTGGGATA ATAAAGTCCA ACCTATGGAA TCCATATTAG CAGACTGGAA TGAAGATATT 
GAAGCATTTG AAATGATGGA AAAGGATGAG CTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011587067.1
CDS	35..2890
Translation	MSGAAGCQGGGGGGERGHRWRRPWKLLALGLLSASSVLAAAPGAGAMSKEEKRRLGNQVLEMFDHAYSNYMEHAYPADELMPLTCRGRVRGQEPSRGDVDDALGKFSLTLIDTLDTLVVLNKTKEFEEAVKKVIKDVNLDNDIVVSVFETNIRVLGGLLGGHSVAIMLKEKGEYMQWYNGELLHMAKELGYKLLPAFNTTSGLPYPRVNLKFGVRHPEARTGTETDTCTACAGTLILEFAALSRFTGTSIFEEYARKALDFIWEKRQRSSNLVGVTINIHTGDWVRKDSGVGAGIDSYYEYLLKAYVLLGDDSFLERFNTHYDAIMRYISQPPLLLDVHIHKPMLNARTWMDSLLAFFPGLQVLKGDIRPAIETHEMLYQVIKKHNFLPEAFTTDFRVHWAQHPLRPEFAESTYFLYKATGDPYYLEVGKTLIENLNKYARVPCGFAAMKDVRTGSHEDRMDSFFLAEMFKYLYLLFADKEDMIFDIEDYIFTTEAHLLPLWLSTTNQTISKKNTTTEYTELDDSNFDWTCPNTQILFPNDPMFAQSIREPLKNVVDKSCPRGISRAEESLGSGPKPPLRARDFMASNPEHLEILKKMGVSLIHLKDGRVQLVQHAVQAASSLDAEDGLRFMQEMIELSSQQQKEQQLPPRAVQIVSHPFFGRVVLTAGPAQFGMDLSKHKAGTRGFVATIKPYNGCSEITNPEAVKEKIALMQRGQCMFAEKARNIQKAGAIGGIVIDDNEGSSSDTAPLFQMAGDGKNTDDITIPMLFLFNKEGNIILDAIREYEAVEVLLSDKAKDRATIFKGKMIPNYIIDSNLEVENMDQKLSENDSHKQNAEEATSASQDVGAVSEEPEEGESSDVTDPDSLSPANADSDSVSISNQDSYVPGTDEAGAPEPACTRGDNQPQEQKTEAESNSKVNWDNKVQPMESILADWNEDIEAFEMMEKDEL

Target ORF information:

RefSeq Version	XM_011588765.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis ER degradation enhancer, mannosidase alpha-like 3 (EDEM3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011588765.1

ORF Insert Sequence:

ATGAGCGGAG CTGCGGGCTG CCAGGGGGGC GGTGGCGGCG GCGAGCGGGG GCACCGCTGG 
AGGAGGCCGT GGAAGCTCCT GGCCCTTGGC CTGCTCTCCG CCTCCTCCGT GCTGGCGGCC 
GCCCCGGGCG CCGGGGCCAT GAGCAAGGAG GAGAAGCGCC GGTTGGGAAA CCAAGTGCTC 
GAAATGTTTG ATCATGCATA TAGCAATTAT ATGGAGCATG CGTATCCAGC TGATGAACTC 
ATGCCTTTAA CTTGTCGTGG ACGAGTACGG GGTCAGGAAC CAAGTCGAGG GGATGTGGAT 
GATGCCTTGG GAAAATTTTC ACTGACCTTG ATTGATACCT TGGACACTCT TGTGGTGTTA 
AATAAAACCA AAGAGTTTGA AGAGGCTGTA AAAAAAGTAA TAAAGGATGT TAATTTAGAT 
AACGACATAG TTGTATCTGT CTTTGAAACC AACATAAGAG TCCTTGGAGG TCTCCTAGGT 
GGGCATTCAG TAGCAATTAT GCTGAAAGAA AAAGGTGAAT ATATGCAGTG GTACAATGGT 
GAACTTCTGC ACATGGCAAA GGAACTAGGC TACAAGCTTT TACCTGCTTT TAACACCACT 
AGCGGTCTCC CTTATCCAAG AGTTAACTTA AAATTTGGTG TAAGACATCC AGAAGCACGA 
ACAGGAACAG AAACTGATAC CTGTACAGCT TGTGCGGGTA CCTTGATCCT TGAGTTTGCA 
GCTTTAAGCC GATTCACGGG AACATCTATA TTTGAGGAGT ATGCACGAAA AGCGCTTGAT 
TTTATTTGGG AAAAGAGACA ACGCAGCAGC AATTTAGTGG GCGTTACTAT AAACATTCAT 
ACTGGAGACT GGGTCCGCAA AGATAGTGGA GTTGGAGCAG GAATAGATTC ATATTATGAA 
TATTTGTTAA AAGCCTATGT CTTGCTGGGA GATGACAGTT TCCTGGAAAG ATTTAACACG 
CACTACGATG CCATAATGAG ATACATTAGC CAACCACCTC TTCTTCTTGA TGTTCATATT 
CATAAACCAA TGCTAAATGC TAGGACCTGG ATGGATTCTT TGCTAGCTTT CTTCCCTGGA 
TTGCAGGTGC TGAAGGGTGA TATTAGACCT GCTATTGAAA CTCATGAGAT GCTGTATCAG 
GTTATAAAAA AGCATAACTT TCTTCCAGAG GCATTCACAA CAGACTTTAG AGTTCACTGG 
GCTCAGCATC CTTTAAGGCC TGAATTTGCT GAAAGCACTT ACTTCTTGTA TAAAGCTACT 
GGTGACCCTT ACTATCTAGA AGTAGGAAAA ACACTCATTG AAAACTTGAA CAAGTATGCA 
AGAGTACCTT GTGGGTTTGC TGCAATGAAG GATGTTCGTA CAGGAAGTCA TGAAGACAGA 
ATGGACTCTT TCTTTCTGGC TGAGATGTTT AAATATCTTT ATCTGCTGTT TGCTGATAAG 
GAAGACATGA TTTTTGACAT TGAAGATTAT ATCTTTACTA CAGAAGCTCA TCTATTACCA 
CTTTGGCTTT CCACCACAAA CCAAACTATC TCTAAAAAAA ATACAACTAC AGAATACACA 
GAGCTGGATG ACAGCAACTT TGACTGGACC TGTCCAAACA CCCAGATTCT TTTCCCAAAT 
GACCCTATGT TTGCTCAAAG TATTCGTGAA CCTTTGAAAA ATGTGGTGGA TAAGAGCTGT 
CCTAGGGGTA TTTCCAGAGC AGAAGAGAGT TTGGGAAGTG GACCAAAACC ACCACTGAGA 
GCCAGAGATT TCATGGCCAG TAATCCTGAA CACTTGGAGA TACTGAAGAA GATGGGAGTC 
AGTTTGATTC ATCTGAAGGA TGGAAGAGTA CAATTAGTAC AGCATGCAGT GCAAGCAGCA 
AGTTCACTTG ATGCTGAAGA TGGCTTACGG TTCATGCAAG AAATGATTGA ACTCTCCAGT 
CAGCAACAGA AAGAGCAACA GCTACCTCCT CGTGCTGTTC AAATTGTTTC CCATCCATTC 
TTTGGCAGGG TCGTCTTGAC TGCTGGACCA GCACAATTTG GGATGGACTT ATCCAAACAC 
AAAGCAGGGA CAAGAGGATT TGTTGCAACC ATTAAGCCAT ATAATGGATG CTCAGAGATC 
ACTAATCCTG AGGCAGTGAA AGAGAAAATT GCTCTGATGC AAAGAGGCCA GTGTATGTTT 
GCTGAAAAGG CACGAAACAT TCAAAAAGCT GGAGCAATAG GAGGCATTGT TATTGATGAC 
AATGAGGGAA GCAGTAGTGA CACAGCTCCT CTCTTCCAAA TGGCCGGTGA TGGAAAAAAC 
ACAGATGATA TCACAATTCC CATGCTCTTC CTCTTCAACA AAGAAGGAAA CATTATACTG 
GATGCAATCC GGGAGTATGA GGCTGTGGAG GTGCTCCTTT CTGATAAAGC AAAAGACAGA 
GCAACAATCT TTAAAGGTAA AATGATTCCA AACTACATTA TTGATAGCAA CTTAGAAGTG 
GAGAATATGG ACCAGAAATT GTCTGAAAAT GATTCACATA AACAGAATGC AGAAGAAGCT 
ACTTCAGCAT CTCAGGATGT TGGTGCAGTC AGTGAGGAGC CTGAAGAAGG AGAAAGCTCT 
GATGTTACTG ACCCTGATTC TTTATCTCCT GCTAATGCAG ACAGTGACAG CGTTTCCATT 
TCAAATCAGG ATTCCTATGT CCCTGGAACC GATGAGGCTG GTGCTCCGGA GCCAGCATGT 
ACGCGAGGGG ATAACCAGCC TCAGGAACAA AAGACTGAGG CAGAGTCAAA TTCCAAAGTT 
AACTGGGATA ATAAAGTCCA ACCTATGGAA TCCATATTAG CAGACTGGAA TGAAGATATT 
GAAGCATTTG AAATGATGGA AAAGGATGAG CTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EDEM3 ( XM_011588766.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011587068.1 Aquila chrysaetos canadensis ER degradation enhancer, mannosidase alpha-like 3 (EDEM3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAq24868
Clone ID Related Accession (Same CDS sequence)	XM_011588766.1
Accession Version	XM_011588766.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011950937.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGAG CTGCGGGCTG CCAGGGGGGC GGTGGCGGCG GCGAGCGGGG GCACCGCTGG 
AGGAGGCCGT GGAAGCTCCT GGCCCTTGGC CTGCTCTCCG CCTCCTCCGT GCTGGCGGCC 
GCCCCGGGCG CCGGGGCCAT GAGCAAGGAG GAGAAGCGCC GGTTGGGAAA CCAAGTGCTC 
GAAATGTTTG ATCATGCATA TAGCAATTAT ATGGAGCATG CGTATCCAGC TGATGAACTC 
ATGCCTTTAA CTTGTCGTGG ACGAGTACGG GGTCAGGAAC CAAGTCGAGG GGATGTGGAT 
GATGCCTTGG GAAAATTTTC ACTGACCTTG ATTGATACCT TGGACACTCT TGTGGTGTTA 
AATAAAACCA AAGAGTTTGA AGAGGCTGTA AAAAAAGTAA TAAAGGATGT TAATTTAGAT 
AACGACATAG TTGTATCTGT CTTTGAAACC AACATAAGAG TCCTTGGAGG TCTCCTAGGT 
GGGCATTCAG TAGCAATTAT GCTGAAAGAA AAAGGTGAAT ATATGCAGTG GTACAATGGT 
GAACTTCTGC ACATGGCAAA GGAACTAGGC TACAAGCTTT TACCTGCTTT TAACACCACT 
AGCGGTCTCC CTTATCCAAG AGTTAACTTA AAATTTGGTG TAAGACATCC AGAAGCACGA 
ACAGGAACAG AAACTGATAC CTGTACAGCT TGTGCGGGTA CCTTGATCCT TGAGTTTGCA 
GCTTTAAGCC GATTCACGGG AACATCTATA TTTGAGGAGT ATGCACGAAA AGCGCTTGAT 
TTTATTTGGG AAAAGAGACA ACGCAGCAGC AATTTAGTGG GCGTTACTAT AAACATTCAT 
ACTGGAGACT GGGTCCGCAA AGATAGTGGA GTTGGAGCAG GAATAGATTC ATATTATGAA 
TATTTGTTAA AAGCCTATGT CTTGCTGGGA GATGACAGTT TCCTGGAAAG ATTTAACACG 
CACTACGATG CCATAATGAG ATACATTAGC CAACCACCTC TTCTTCTTGA TGTTCATATT 
CATAAACCAA TGCTAAATGC TAGGACCTGG ATGGATTCTT TGCTAGCTTT CTTCCCTGGA 
TTGCAGGTGC TGAAGGGTGA TATTAGACCT GCTATTGAAA CTCATGAGAT GCTGTATCAG 
GTTATAAAAA AGCATAACTT TCTTCCAGAG GCATTCACAA CAGACTTTAG AGTTCACTGG 
GCTCAGCATC CTTTAAGGCC TGAATTTGCT GAAAGCACTT ACTTCTTGTA TAAAGCTACT 
GGTGACCCTT ACTATCTAGA AGTAGGAAAA ACACTCATTG AAAACTTGAA CAAGTATGCA 
AGAGTACCTT GTGGGTTTGC TGCAATGAAG GATGTTCGTA CAGGAAGTCA TGAAGACAGA 
ATGGACTCTT TCTTTCTGGC TGAGATGTTT AAATATCTTT ATCTGCTGTT TGCTGATAAG 
GAAGACATGA TTTTTGACAT TGAAGATTAT ATCTTTACTA CAGAAGCTCA TCTATTACCA 
CTTTGGCTTT CCACCACAAA CCAAACTATC TCTAAAAAAA ATACAACTAC AGAATACACA 
GAGCTGGATG ACAGCAACTT TGACTGGACC TGTCCAAACA CCCAGATTCT TTTCCCAAAT 
GACCCTATGT TTGCTCAAAG TATTCGTGAA CCTTTGAAAA ATGTGGTGGA TAAGAGCTGT 
CCTAGGGGTA TTTCCAGAGC AGAAGAGAGT TTGGGAAGTG GACCAAAACC ACCACTGAGA 
GCCAGAGATT TCATGGCCAG TAATCCTGAA CACTTGGAGA TACTGAAGAA GATGGGAGTC 
AGTTTGATTC ATCTGAAGGA TGGAAGAGTA CAATTAGTAC AGCATGCAGT GCAAGCAGCA 
AGTTCACTTG ATGCTGAAGA TGGCTTACGG TTCATGCAAG AAATGATTGA ACTCTCCAGT 
CAGCAACAGA AAGAGCAACA GCTACCTCCT CGTGCTGTTC AAATTGTTTC CCATCCATTC 
TTTGGCAGGG TCGTCTTGAC TGCTGGACCA GCACAATTTG GGATGGACTT ATCCAAACAC 
AAAGCAGGGA CAAGAGGATT TGTTGCAACC ATTAAGCCAT ATAATGGATG CTCAGAGATC 
ACTAATCCTG AGGCAGTGAA AGAGAAAATT GCTCTGATGC AAAGAGGCCA GTGTATGTTT 
GCTGAAAAGG CACGAAACAT TCAAAAAGCT GGAGCAATAG GAGGCATTGT TATTGATGAC 
AATGAGGGAA GCAGTAGTGA CACAGCTCCT CTCTTCCAAA TGGCCGGTGA TGGAAAAAAC 
ACAGATGATA TCACAATTCC CATGCTCTTC CTCTTCAACA AAGAAGGAAA CATTATACTG 
GATGCAATCC GGGAGTATGA GGCTGTGGAG GTGCTCCTTT CTGATAAAGC AAAAGACAGA 
GACTTAGAAG TGGAGAATAT GGACCAGAAA TTGTCTGAAA ATGATTCACA TAAACAGAAT 
GCAGAAGAAG CTACTTCAGC ATCTCAGGAT GTTGGTGCAG TCAGTGAGGA GCCTGAAGAA 
GGAGAAAGCT CTGATGTTAC TGACCCTGAT TCTTTATCTC CTGCTAATGC AGACAGTGAC 
AGCGTTTCCA TTTCAAATCA GGATTCCTAT GTCCCTGGAA CCGATGAGGC TGGTGCTCCG 
GAGCCAGCAT GTACGCGAGG GGATAACCAG CCTCAGGAAC AAAAGACTGA GGCAGAGTCA 
AATTCCAAAG TTAACTGGGA TAATAAAGTC CAACCTATGG AATCCATATT AGCAGACTGG 
AATGAAGATA TTGAAGCATT TGAAATGATG GAAAAGGATG AGCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011587068.1
CDS	35..2842
Translation	MSGAAGCQGGGGGGERGHRWRRPWKLLALGLLSASSVLAAAPGAGAMSKEEKRRLGNQVLEMFDHAYSNYMEHAYPADELMPLTCRGRVRGQEPSRGDVDDALGKFSLTLIDTLDTLVVLNKTKEFEEAVKKVIKDVNLDNDIVVSVFETNIRVLGGLLGGHSVAIMLKEKGEYMQWYNGELLHMAKELGYKLLPAFNTTSGLPYPRVNLKFGVRHPEARTGTETDTCTACAGTLILEFAALSRFTGTSIFEEYARKALDFIWEKRQRSSNLVGVTINIHTGDWVRKDSGVGAGIDSYYEYLLKAYVLLGDDSFLERFNTHYDAIMRYISQPPLLLDVHIHKPMLNARTWMDSLLAFFPGLQVLKGDIRPAIETHEMLYQVIKKHNFLPEAFTTDFRVHWAQHPLRPEFAESTYFLYKATGDPYYLEVGKTLIENLNKYARVPCGFAAMKDVRTGSHEDRMDSFFLAEMFKYLYLLFADKEDMIFDIEDYIFTTEAHLLPLWLSTTNQTISKKNTTTEYTELDDSNFDWTCPNTQILFPNDPMFAQSIREPLKNVVDKSCPRGISRAEESLGSGPKPPLRARDFMASNPEHLEILKKMGVSLIHLKDGRVQLVQHAVQAASSLDAEDGLRFMQEMIELSSQQQKEQQLPPRAVQIVSHPFFGRVVLTAGPAQFGMDLSKHKAGTRGFVATIKPYNGCSEITNPEAVKEKIALMQRGQCMFAEKARNIQKAGAIGGIVIDDNEGSSSDTAPLFQMAGDGKNTDDITIPMLFLFNKEGNIILDAIREYEAVEVLLSDKAKDRDLEVENMDQKLSENDSHKQNAEEATSASQDVGAVSEEPEEGESSDVTDPDSLSPANADSDSVSISNQDSYVPGTDEAGAPEPACTRGDNQPQEQKTEAESNSKVNWDNKVQPMESILADWNEDIEAFEMMEKDEL

Target ORF information:

RefSeq Version	XM_011588766.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis ER degradation enhancer, mannosidase alpha-like 3 (EDEM3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011588766.1

ORF Insert Sequence:

ATGAGCGGAG CTGCGGGCTG CCAGGGGGGC GGTGGCGGCG GCGAGCGGGG GCACCGCTGG 
AGGAGGCCGT GGAAGCTCCT GGCCCTTGGC CTGCTCTCCG CCTCCTCCGT GCTGGCGGCC 
GCCCCGGGCG CCGGGGCCAT GAGCAAGGAG GAGAAGCGCC GGTTGGGAAA CCAAGTGCTC 
GAAATGTTTG ATCATGCATA TAGCAATTAT ATGGAGCATG CGTATCCAGC TGATGAACTC 
ATGCCTTTAA CTTGTCGTGG ACGAGTACGG GGTCAGGAAC CAAGTCGAGG GGATGTGGAT 
GATGCCTTGG GAAAATTTTC ACTGACCTTG ATTGATACCT TGGACACTCT TGTGGTGTTA 
AATAAAACCA AAGAGTTTGA AGAGGCTGTA AAAAAAGTAA TAAAGGATGT TAATTTAGAT 
AACGACATAG TTGTATCTGT CTTTGAAACC AACATAAGAG TCCTTGGAGG TCTCCTAGGT 
GGGCATTCAG TAGCAATTAT GCTGAAAGAA AAAGGTGAAT ATATGCAGTG GTACAATGGT 
GAACTTCTGC ACATGGCAAA GGAACTAGGC TACAAGCTTT TACCTGCTTT TAACACCACT 
AGCGGTCTCC CTTATCCAAG AGTTAACTTA AAATTTGGTG TAAGACATCC AGAAGCACGA 
ACAGGAACAG AAACTGATAC CTGTACAGCT TGTGCGGGTA CCTTGATCCT TGAGTTTGCA 
GCTTTAAGCC GATTCACGGG AACATCTATA TTTGAGGAGT ATGCACGAAA AGCGCTTGAT 
TTTATTTGGG AAAAGAGACA ACGCAGCAGC AATTTAGTGG GCGTTACTAT AAACATTCAT 
ACTGGAGACT GGGTCCGCAA AGATAGTGGA GTTGGAGCAG GAATAGATTC ATATTATGAA 
TATTTGTTAA AAGCCTATGT CTTGCTGGGA GATGACAGTT TCCTGGAAAG ATTTAACACG 
CACTACGATG CCATAATGAG ATACATTAGC CAACCACCTC TTCTTCTTGA TGTTCATATT 
CATAAACCAA TGCTAAATGC TAGGACCTGG ATGGATTCTT TGCTAGCTTT CTTCCCTGGA 
TTGCAGGTGC TGAAGGGTGA TATTAGACCT GCTATTGAAA CTCATGAGAT GCTGTATCAG 
GTTATAAAAA AGCATAACTT TCTTCCAGAG GCATTCACAA CAGACTTTAG AGTTCACTGG 
GCTCAGCATC CTTTAAGGCC TGAATTTGCT GAAAGCACTT ACTTCTTGTA TAAAGCTACT 
GGTGACCCTT ACTATCTAGA AGTAGGAAAA ACACTCATTG AAAACTTGAA CAAGTATGCA 
AGAGTACCTT GTGGGTTTGC TGCAATGAAG GATGTTCGTA CAGGAAGTCA TGAAGACAGA 
ATGGACTCTT TCTTTCTGGC TGAGATGTTT AAATATCTTT ATCTGCTGTT TGCTGATAAG 
GAAGACATGA TTTTTGACAT TGAAGATTAT ATCTTTACTA CAGAAGCTCA TCTATTACCA 
CTTTGGCTTT CCACCACAAA CCAAACTATC TCTAAAAAAA ATACAACTAC AGAATACACA 
GAGCTGGATG ACAGCAACTT TGACTGGACC TGTCCAAACA CCCAGATTCT TTTCCCAAAT 
GACCCTATGT TTGCTCAAAG TATTCGTGAA CCTTTGAAAA ATGTGGTGGA TAAGAGCTGT 
CCTAGGGGTA TTTCCAGAGC AGAAGAGAGT TTGGGAAGTG GACCAAAACC ACCACTGAGA 
GCCAGAGATT TCATGGCCAG TAATCCTGAA CACTTGGAGA TACTGAAGAA GATGGGAGTC 
AGTTTGATTC ATCTGAAGGA TGGAAGAGTA CAATTAGTAC AGCATGCAGT GCAAGCAGCA 
AGTTCACTTG ATGCTGAAGA TGGCTTACGG TTCATGCAAG AAATGATTGA ACTCTCCAGT 
CAGCAACAGA AAGAGCAACA GCTACCTCCT CGTGCTGTTC AAATTGTTTC CCATCCATTC 
TTTGGCAGGG TCGTCTTGAC TGCTGGACCA GCACAATTTG GGATGGACTT ATCCAAACAC 
AAAGCAGGGA CAAGAGGATT TGTTGCAACC ATTAAGCCAT ATAATGGATG CTCAGAGATC 
ACTAATCCTG AGGCAGTGAA AGAGAAAATT GCTCTGATGC AAAGAGGCCA GTGTATGTTT 
GCTGAAAAGG CACGAAACAT TCAAAAAGCT GGAGCAATAG GAGGCATTGT TATTGATGAC 
AATGAGGGAA GCAGTAGTGA CACAGCTCCT CTCTTCCAAA TGGCCGGTGA TGGAAAAAAC 
ACAGATGATA TCACAATTCC CATGCTCTTC CTCTTCAACA AAGAAGGAAA CATTATACTG 
GATGCAATCC GGGAGTATGA GGCTGTGGAG GTGCTCCTTT CTGATAAAGC AAAAGACAGA 
GACTTAGAAG TGGAGAATAT GGACCAGAAA TTGTCTGAAA ATGATTCACA TAAACAGAAT 
GCAGAAGAAG CTACTTCAGC ATCTCAGGAT GTTGGTGCAG TCAGTGAGGA GCCTGAAGAA 
GGAGAAAGCT CTGATGTTAC TGACCCTGAT TCTTTATCTC CTGCTAATGC AGACAGTGAC 
AGCGTTTCCA TTTCAAATCA GGATTCCTAT GTCCCTGGAA CCGATGAGGC TGGTGCTCCG 
GAGCCAGCAT GTACGCGAGG GGATAACCAG CCTCAGGAAC AAAAGACTGA GGCAGAGTCA 
AATTCCAAAG TTAACTGGGA TAATAAAGTC CAACCTATGG AATCCATATT AGCAGACTGG 
AATGAAGATA TTGAAGCATT TGAAATGATG GAAAAGGATG AGCTATGA