LOC109728590 cDNA ORF clone, Ananas comosus(pineapple)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC109728590
Entrez Gene ID	109728590
Full Name	tRNA (cytosine(34)-C(5))-methyltransferase-like
Gene Type	protein-coding
Organism	Ananas comosus(pineapple)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020259038.1	XP_020114627.1	tRNA (cytosine(34)-C(5))-methyltransferase-like isoform X1
XM_020259043.1	XP_020114632.1	tRNA (cytosine(34)-C(5))-methyltransferase-like isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC109728590 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC109728590 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAn60543	XM_020259038.1 Latest version!	Ananas comosus tRNA (cytosine(34)-C(5))-methyltransferase-like (LOC109728590), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OAn60544	XM_020259043.1 Latest version!	Ananas comosus tRNA (cytosine(34)-C(5))-methyltransferase-like (LOC109728590), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC109728590 ( XM_020259038.1 ) cDNA ORF clone, Ananas comosus(pineapple) -> XP_020114627.1 Ananas comosus tRNA (cytosine(34)-C(5))-methyltransferase-like (LOC109728590), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAn60543
Clone ID Related Accession (Same CDS sequence)	XM_020259038.1
Accession Version	XM_020259038.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-14
Organism	Ananas comosus(pineapple)
Product	tRNA (cytosine(34)-C(5))-methyltransferase-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033622.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ananas comosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGTG GGCAGAAGCG CGGCCGCACG CAGCGGCGGC ACTTCAAGCA GAGCCGCGAG 
AACGTGTGGA AGCTCAACCC CAAGAAGACC CGCCCCGACG CCGACGAACC CGACGCCGAC 
GCCAAAGCCA ACGCCAACCC CAAGTGGGAG CCCTTCGCCA CCCAAAACCC CGGCTTCGAC 
GAGTACTACA AGGAGCAAGG CATTGTTCCT GAAGATGAAT GGGATGATTT CATTTCTTTG 
CTCCGAAAGC CTCTGCCTGC AGCATTTAGA ATCAATGCAA GTGGCCAATT TTTTCAGGAC 
ATTCGATCAC AGTTGGAAAA TGACTTCATG AAATCACTGC AGGCTGAGGT AACAGATGAT 
TATGAAGCGG AAGCTATTAG ACCATTGTCA TGGTATCCAG GGAACATTGC ATGGCATTTG 
AACTTTTCTC GTATGCAACT TAGGAAGAAC CAGGCACTTG AGAGGTTCCA TGAGTTTTTA 
AAGCAAGAGA ATGAGATTGG GAACATAACA AGGCAAGAGG CTGTCAGCAT GGTTCCTCCA 
CTCTTCCTTG ATGTACTACC GGATCACCAC ATTCTTGACA TGTGTGCTGC TCCAGGATCT 
AAAACTTTCC AGTTGCTTGA AATTATGCAC CAATCAATAG GACCCGGAGT ACTTCCCAAT 
GGGCTGGTGA TAGCTAATGA CGTCGATGTT CAAAGATGTA ATCTCCTCAT CCATCAAACA 
AAGAGGATGT CCACTGCCAA CTTGATAGTA ACAAATCATG AAGCCCAACA TTTTCCCAGT 
TGTTCTTTGA AAAAGGGTCA AGGAGTTGCT TCTGAAGGGC AGGAGGAGCA TGGAATACAT 
ACATTAGAGT TTGACCGTGT TTTATGTGAT GTTCCTTGTA GTGGTGATGG CACCCTGCGC 
AAGGCTCCTG ATATCTGGAG AAAATGGAAT GCAGGCATGG GAAATGGTCT TCATCGTCTC 
CAAGTGGAGA TTGCTATGCG TGGTTTAGCT TTGCTTAAAG TGGGTGGAAG GATGGTCTAC 
TCTACCTGTT CACTGAACCC AGTTGAAAAT GAAGCTGTTG TTGCTGAGGT TCTTCGAAGA 
AGTAGGGGAT CAGTTGAGCT TCTTGATGTC TCCAATGAGC TACCTGAATT GGTGCGCAGG 
CCCGGTCTTA ATACATGGAA GGTACGTGAT AGAGGAGTTT GGCTAACCTC TTATAAGGAT 
GTTCCCCTCT ATAGGCGAAG TGTAATATTA CCAAGCATGT TTCCTTCTGG TCAAAGCTGC 
AAAGAAAACC ATACACTATG CAATGATGTC CAAAAGGATG TAAAAATAGT TGGTAATAAC 
TTAGGGGAGG AAACTGTAGG AGTAGTAGTT ATAGAACAGG ATCAGACAAA TACGGAGCAT 
CAAAATTCTT CTGATGATTT AAGCAGCAAT ACCATTCCAT TGGACACTGT CAAAGCTGCA 
GCTATGGCCA ATGAAGTCTC CAAAAAAAGT ATACCTCCTG AGGAATTGGA TAAGAATTCC 
ATTGCAATGG AAGTTTCAGG TTTTCCACTC CATTATTGCA TGAGAATAGT TCCTCATGAT 
CAAAACAGCG GAGCATTTTT TATTGCTGTT CTTCAAAAGA TGGCCCCTTT GCATGTAGTT 
CCGGAGAATC ATGCAAGTGA GCCAGCTAAA TTTAGTCAGG TATCAGAAAA TACCAGTGAG 
AACCCCGAGG ATGAAAATGA AGCAGAAGAA AGGATATCTG GACAAGTCTC AATTCAAGCA 
GAAGAAAATA CTGACACGAA TGTTCTAGAG GAGCAACTGA AATCAAATTT AAGTACTAAA 
AATGCTTGCT CTACAGCTAA TAACAAAGAG GAAGTGGTTA AGTGTGACGG TGGGAAGAGT 
GGTCACATGG GGATTAAGGA TGTAGAGAGG AAAGGCAAGT GGCGAGGGGT TGATCCTGTT 
GTTTTTTTCA CAGATGAAAA AACCATTAAC AGCATACTTT CTTTCTATGG TATCAATGAT 
GCATTTCCAC TCTATGGTCA CCTTGTGACT CGAAATAGTG ATGCTAATCA TGTGAAGAGA 
ATATACTACA TATCAAATTC GGTTCAGGAA GTCCTACAGC TGAATGATCA AGTTGGTCAG 
CGGCTTAAGA TTACTTCCCT TGGTTTGAAG ATTTTTGAGA GACAAACATC AAAGGATGGA 
TCAACATGTG CATTCCGGTT ATCATCAGAA GGATTACCGT TGCTACTTCC ATATATCAGC 
AAGCAGATTT TGTTTGCATC TTTAGCGGAC TTTCAGCACC TTTTACAGTA CAGGACGATC 
AAATTTCCAG ATTTTGTTGA TTCTAAATTT GGCGAAGAAG CGTCAGACTT AGTTCCTGGT 
TGCTGTGTTG TAGTGCTACG TGAAGGAAAT CGGAGTACAG ATTCTACTGC AGTGGATGCC 
TCTACAGTCG CCATTGTTTG CTGGAAAGGC AAGACAAATA TTAGTATCAT GGTTTCCCCC 
TTGGACGGGA AGGAACTTCT CGAAAGACTC GCGTTTCGGT TCGGAATTAA GAGCATCTCA 
CTAGAAGAGA ATAAAAAATC AAACATGGAA ATTGAGGGGG TAAGCAACTT GGAGGATGTT 
GAACCTATTG CGACCATCGA GACAGATAAC CAAGAAATCG AGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020114627.1
CDS	198..2825
Translation	MGGGQKRGRTQRRHFKQSRENVWKLNPKKTRPDADEPDADAKANANPKWEPFATQNPGFDEYYKEQGIVPEDEWDDFISLLRKPLPAAFRINASGQFFQDIRSQLENDFMKSLQAEVTDDYEAEAIRPLSWYPGNIAWHLNFSRMQLRKNQALERFHEFLKQENEIGNITRQEAVSMVPPLFLDVLPDHHILDMCAAPGSKTFQLLEIMHQSIGPGVLPNGLVIANDVDVQRCNLLIHQTKRMSTANLIVTNHEAQHFPSCSLKKGQGVASEGQEEHGIHTLEFDRVLCDVPCSGDGTLRKAPDIWRKWNAGMGNGLHRLQVEIAMRGLALLKVGGRMVYSTCSLNPVENEAVVAEVLRRSRGSVELLDVSNELPELVRRPGLNTWKVRDRGVWLTSYKDVPLYRRSVILPSMFPSGQSCKENHTLCNDVQKDVKIVGNNLGEETVGVVVIEQDQTNTEHQNSSDDLSSNTIPLDTVKAAAMANEVSKKSIPPEELDKNSIAMEVSGFPLHYCMRIVPHDQNSGAFFIAVLQKMAPLHVVPENHASEPAKFSQVSENTSENPEDENEAEERISGQVSIQAEENTDTNVLEEQLKSNLSTKNACSTANNKEEVVKCDGGKSGHMGIKDVERKGKWRGVDPVVFFTDEKTINSILSFYGINDAFPLYGHLVTRNSDANHVKRIYYISNSVQEVLQLNDQVGQRLKITSLGLKIFERQTSKDGSTCAFRLSSEGLPLLLPYISKQILFASLADFQHLLQYRTIKFPDFVDSKFGEEASDLVPGCCVVVLREGNRSTDSTAVDASTVAIVCWKGKTNISIMVSPLDGKELLERLAFRFGIKSISLEENKKSNMEIEGVSNLEDVEPIATIETDNQEIEH

Target ORF information:

RefSeq Version	XM_020259038.1
Organism	Ananas comosus(pineapple)
Definition	Ananas comosus tRNA (cytosine(34)-C(5))-methyltransferase-like (LOC109728590), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020259038.1

ORF Insert Sequence:

ATGGGCGGTG GGCAGAAGCG CGGCCGCACG CAGCGGCGGC ACTTCAAGCA GAGCCGCGAG 
AACGTGTGGA AGCTCAACCC CAAGAAGACC CGCCCCGACG CCGACGAACC CGACGCCGAC 
GCCAAAGCCA ACGCCAACCC CAAGTGGGAG CCCTTCGCCA CCCAAAACCC CGGCTTCGAC 
GAGTACTACA AGGAGCAAGG CATTGTTCCT GAAGATGAAT GGGATGATTT CATTTCTTTG 
CTCCGAAAGC CTCTGCCTGC AGCATTTAGA ATCAATGCAA GTGGCCAATT TTTTCAGGAC 
ATTCGATCAC AGTTGGAAAA TGACTTCATG AAATCACTGC AGGCTGAGGT AACAGATGAT 
TATGAAGCGG AAGCTATTAG ACCATTGTCA TGGTATCCAG GGAACATTGC ATGGCATTTG 
AACTTTTCTC GTATGCAACT TAGGAAGAAC CAGGCACTTG AGAGGTTCCA TGAGTTTTTA 
AAGCAAGAGA ATGAGATTGG GAACATAACA AGGCAAGAGG CTGTCAGCAT GGTTCCTCCA 
CTCTTCCTTG ATGTACTACC GGATCACCAC ATTCTTGACA TGTGTGCTGC TCCAGGATCT 
AAAACTTTCC AGTTGCTTGA AATTATGCAC CAATCAATAG GACCCGGAGT ACTTCCCAAT 
GGGCTGGTGA TAGCTAATGA CGTCGATGTT CAAAGATGTA ATCTCCTCAT CCATCAAACA 
AAGAGGATGT CCACTGCCAA CTTGATAGTA ACAAATCATG AAGCCCAACA TTTTCCCAGT 
TGTTCTTTGA AAAAGGGTCA AGGAGTTGCT TCTGAAGGGC AGGAGGAGCA TGGAATACAT 
ACATTAGAGT TTGACCGTGT TTTATGTGAT GTTCCTTGTA GTGGTGATGG CACCCTGCGC 
AAGGCTCCTG ATATCTGGAG AAAATGGAAT GCAGGCATGG GAAATGGTCT TCATCGTCTC 
CAAGTGGAGA TTGCTATGCG TGGTTTAGCT TTGCTTAAAG TGGGTGGAAG GATGGTCTAC 
TCTACCTGTT CACTGAACCC AGTTGAAAAT GAAGCTGTTG TTGCTGAGGT TCTTCGAAGA 
AGTAGGGGAT CAGTTGAGCT TCTTGATGTC TCCAATGAGC TACCTGAATT GGTGCGCAGG 
CCCGGTCTTA ATACATGGAA GGTACGTGAT AGAGGAGTTT GGCTAACCTC TTATAAGGAT 
GTTCCCCTCT ATAGGCGAAG TGTAATATTA CCAAGCATGT TTCCTTCTGG TCAAAGCTGC 
AAAGAAAACC ATACACTATG CAATGATGTC CAAAAGGATG TAAAAATAGT TGGTAATAAC 
TTAGGGGAGG AAACTGTAGG AGTAGTAGTT ATAGAACAGG ATCAGACAAA TACGGAGCAT 
CAAAATTCTT CTGATGATTT AAGCAGCAAT ACCATTCCAT TGGACACTGT CAAAGCTGCA 
GCTATGGCCA ATGAAGTCTC CAAAAAAAGT ATACCTCCTG AGGAATTGGA TAAGAATTCC 
ATTGCAATGG AAGTTTCAGG TTTTCCACTC CATTATTGCA TGAGAATAGT TCCTCATGAT 
CAAAACAGCG GAGCATTTTT TATTGCTGTT CTTCAAAAGA TGGCCCCTTT GCATGTAGTT 
CCGGAGAATC ATGCAAGTGA GCCAGCTAAA TTTAGTCAGG TATCAGAAAA TACCAGTGAG 
AACCCCGAGG ATGAAAATGA AGCAGAAGAA AGGATATCTG GACAAGTCTC AATTCAAGCA 
GAAGAAAATA CTGACACGAA TGTTCTAGAG GAGCAACTGA AATCAAATTT AAGTACTAAA 
AATGCTTGCT CTACAGCTAA TAACAAAGAG GAAGTGGTTA AGTGTGACGG TGGGAAGAGT 
GGTCACATGG GGATTAAGGA TGTAGAGAGG AAAGGCAAGT GGCGAGGGGT TGATCCTGTT 
GTTTTTTTCA CAGATGAAAA AACCATTAAC AGCATACTTT CTTTCTATGG TATCAATGAT 
GCATTTCCAC TCTATGGTCA CCTTGTGACT CGAAATAGTG ATGCTAATCA TGTGAAGAGA 
ATATACTACA TATCAAATTC GGTTCAGGAA GTCCTACAGC TGAATGATCA AGTTGGTCAG 
CGGCTTAAGA TTACTTCCCT TGGTTTGAAG ATTTTTGAGA GACAAACATC AAAGGATGGA 
TCAACATGTG CATTCCGGTT ATCATCAGAA GGATTACCGT TGCTACTTCC ATATATCAGC 
AAGCAGATTT TGTTTGCATC TTTAGCGGAC TTTCAGCACC TTTTACAGTA CAGGACGATC 
AAATTTCCAG ATTTTGTTGA TTCTAAATTT GGCGAAGAAG CGTCAGACTT AGTTCCTGGT 
TGCTGTGTTG TAGTGCTACG TGAAGGAAAT CGGAGTACAG ATTCTACTGC AGTGGATGCC 
TCTACAGTCG CCATTGTTTG CTGGAAAGGC AAGACAAATA TTAGTATCAT GGTTTCCCCC 
TTGGACGGGA AGGAACTTCT CGAAAGACTC GCGTTTCGGT TCGGAATTAA GAGCATCTCA 
CTAGAAGAGA ATAAAAAATC AAACATGGAA ATTGAGGGGG TAAGCAACTT GGAGGATGTT 
GAACCTATTG CGACCATCGA GACAGATAAC CAAGAAATCG AGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC109728590 ( XM_020259043.1 ) cDNA ORF clone, Ananas comosus(pineapple) -> XP_020114632.1 Ananas comosus tRNA (cytosine(34)-C(5))-methyltransferase-like (LOC109728590), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAn60544
Clone ID Related Accession (Same CDS sequence)	XM_020259043.1
Accession Version	XM_020259043.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2625bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-14
Organism	Ananas comosus(pineapple)
Product	tRNA (cytosine(34)-C(5))-methyltransferase-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033622.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ananas comosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGTG GGCAGAAGCG CGGCCGCACG CAGCGGCGGC ACTTCAAGCA GAGCCGCGAG 
AACGTGTGGA AGCTCAACCC CAAGAAGACC CGCCCCGACG CCGACGAACC CGACGCCGAC 
GCCAAAGCCA ACGCCAACCC CAAGTGGGAG CCCTTCGCCA CCCAAAACCC CGGCTTCGAC 
GAGTACTACA AGGAGCAAGG CATTGTTCCT GAAGATGAAT GGGATGATTT CATTTCTTTG 
CTCCGAAAGC CTCTGCCTGC AGCATTTAGA ATCAATGCAA GTGGCCAATT TTTTCAGGAC 
ATTCGATCAC AGTTGGAAAA TGACTTCATG AAATCACTGC AGGCTGAGGT AACAGATGAT 
TATGAAGCGG AAGCTATTAG ACCATTGTCA TGGTATCCAG GGAACATTGC ATGGCATTTG 
AACTTTTCTC GTATGCAACT TAGGAAGAAC CAGGCACTTG AGAGGTTCCA TGAGTTTTTA 
AAGCAAGAGA ATGAGATTGG GAACATAACA AGGCAAGAGG CTGTCAGCAT GGTTCCTCCA 
CTCTTCCTTG ATGTACTACC GGATCACCAC ATTCTTGACA TGTGTGCTGC TCCAGGATCT 
AAAACTTTCC AGTTGCTTGA AATTATGCAC CAATCAATAG GACCCGGAGT ACTTCCCAAT 
GGGCTGGTGA TAGCTAATGA CGTCGATGTT CAAAGATGTA ATCTCCTCAT CCATCAAACA 
AAGAGGATGT CCACTGCCAA CTTGATAGTA ACAAATCATG AAGCCCAACA TTTTCCCAGT 
TGTTCTTTGA AAAAGGGTCA AGGAGTTGCT TCTGAAGGGC AGGAGGAGCA TGGAATACAT 
ACATTAGAGT TTGACCGTGT TTTATGTGAT GTTCCTTGTA GTGGTGATGG CACCCTGCGC 
AAGGCTCCTG ATATCTGGAG AAAATGGAAT GCAGGCATGG GAAATGGTCT TCATCGTCTC 
CAAGTGGAGA TTGCTATGCG TGGTTTAGCT TTGCTTAAAG TGGGTGGAAG GATGGTCTAC 
TCTACCTGTT CACTGAACCC AGTTGAAAAT GAAGCTGTTG TTGCTGAGGT TCTTCGAAGA 
AGTAGGGGAT CAGTTGAGCT TCTTGATGTC TCCAATGAGC TACCTGAATT GGTGCGCAGG 
CCCGGTCTTA ATACATGGAA GGTACGTGAT AGAGGAGTTT GGCTAACCTC TTATAAGGAT 
GTTCCCCTCT ATAGGCGAAG TGTAATATTA CCAAGCATGT TTCCTTCTGG TCAAAGCTGC 
AAAGAAAACC ATACACTATG CAATGATGTC CAAAAGGATG TAAAAATAGT TGGTAATAAC 
TTAGGGGAGG AAACTGTAGG AGTAGTAGTT ATAGAACAGG ATCAGACAAA TACGGAGCAT 
CAAAATTCTT CTGATGATTT AAGCAGCAAT ACCATTCCAT TGGACACTGT CAAAGCTGCA 
GCTATGGCCA ATGAAGTCTC CAAAAAAAGT ATACCTCCTG AGGAATTGGA TAAGAATTCC 
ATTGCAATGG AAGTTTCAGG TTTTCCACTC CATTATTGCA TGAGAATAGT TCCTCATGAT 
CAAAACAGCG GAGCATTTTT TATTGCTGTT CTTCAAAAGA TGGCCCCTTT GCATGTTCCG 
GAGAATCATG CAAGTGAGCC AGCTAAATTT AGTCAGGTAT CAGAAAATAC CAGTGAGAAC 
CCCGAGGATG AAAATGAAGC AGAAGAAAGG ATATCTGGAC AAGTCTCAAT TCAAGCAGAA 
GAAAATACTG ACACGAATGT TCTAGAGGAG CAACTGAAAT CAAATTTAAG TACTAAAAAT 
GCTTGCTCTA CAGCTAATAA CAAAGAGGAA GTGGTTAAGT GTGACGGTGG GAAGAGTGGT 
CACATGGGGA TTAAGGATGT AGAGAGGAAA GGCAAGTGGC GAGGGGTTGA TCCTGTTGTT 
TTTTTCACAG ATGAAAAAAC CATTAACAGC ATACTTTCTT TCTATGGTAT CAATGATGCA 
TTTCCACTCT ATGGTCACCT TGTGACTCGA AATAGTGATG CTAATCATGT GAAGAGAATA 
TACTACATAT CAAATTCGGT TCAGGAAGTC CTACAGCTGA ATGATCAAGT TGGTCAGCGG 
CTTAAGATTA CTTCCCTTGG TTTGAAGATT TTTGAGAGAC AAACATCAAA GGATGGATCA 
ACATGTGCAT TCCGGTTATC ATCAGAAGGA TTACCGTTGC TACTTCCATA TATCAGCAAG 
CAGATTTTGT TTGCATCTTT AGCGGACTTT CAGCACCTTT TACAGTACAG GACGATCAAA 
TTTCCAGATT TTGTTGATTC TAAATTTGGC GAAGAAGCGT CAGACTTAGT TCCTGGTTGC 
TGTGTTGTAG TGCTACGTGA AGGAAATCGG AGTACAGATT CTACTGCAGT GGATGCCTCT 
ACAGTCGCCA TTGTTTGCTG GAAAGGCAAG ACAAATATTA GTATCATGGT TTCCCCCTTG 
GACGGGAAGG AACTTCTCGA AAGACTCGCG TTTCGGTTCG GAATTAAGAG CATCTCACTA 
GAAGAGAATA AAAAATCAAA CATGGAAATT GAGGGGGTAA GCAACTTGGA GGATGTTGAA 
CCTATTGCGA CCATCGAGAC AGATAACCAA GAAATCGAGC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020114632.1
CDS	199..2823
Translation	MGGGQKRGRTQRRHFKQSRENVWKLNPKKTRPDADEPDADAKANANPKWEPFATQNPGFDEYYKEQGIVPEDEWDDFISLLRKPLPAAFRINASGQFFQDIRSQLENDFMKSLQAEVTDDYEAEAIRPLSWYPGNIAWHLNFSRMQLRKNQALERFHEFLKQENEIGNITRQEAVSMVPPLFLDVLPDHHILDMCAAPGSKTFQLLEIMHQSIGPGVLPNGLVIANDVDVQRCNLLIHQTKRMSTANLIVTNHEAQHFPSCSLKKGQGVASEGQEEHGIHTLEFDRVLCDVPCSGDGTLRKAPDIWRKWNAGMGNGLHRLQVEIAMRGLALLKVGGRMVYSTCSLNPVENEAVVAEVLRRSRGSVELLDVSNELPELVRRPGLNTWKVRDRGVWLTSYKDVPLYRRSVILPSMFPSGQSCKENHTLCNDVQKDVKIVGNNLGEETVGVVVIEQDQTNTEHQNSSDDLSSNTIPLDTVKAAAMANEVSKKSIPPEELDKNSIAMEVSGFPLHYCMRIVPHDQNSGAFFIAVLQKMAPLHVPENHASEPAKFSQVSENTSENPEDENEAEERISGQVSIQAEENTDTNVLEEQLKSNLSTKNACSTANNKEEVVKCDGGKSGHMGIKDVERKGKWRGVDPVVFFTDEKTINSILSFYGINDAFPLYGHLVTRNSDANHVKRIYYISNSVQEVLQLNDQVGQRLKITSLGLKIFERQTSKDGSTCAFRLSSEGLPLLLPYISKQILFASLADFQHLLQYRTIKFPDFVDSKFGEEASDLVPGCCVVVLREGNRSTDSTAVDASTVAIVCWKGKTNISIMVSPLDGKELLERLAFRFGIKSISLEENKKSNMEIEGVSNLEDVEPIATIETDNQEIEH

Target ORF information:

RefSeq Version	XM_020259043.1
Organism	Ananas comosus(pineapple)
Definition	Ananas comosus tRNA (cytosine(34)-C(5))-methyltransferase-like (LOC109728590), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020259043.1

ORF Insert Sequence:

ATGGGCGGTG GGCAGAAGCG CGGCCGCACG CAGCGGCGGC ACTTCAAGCA GAGCCGCGAG 
AACGTGTGGA AGCTCAACCC CAAGAAGACC CGCCCCGACG CCGACGAACC CGACGCCGAC 
GCCAAAGCCA ACGCCAACCC CAAGTGGGAG CCCTTCGCCA CCCAAAACCC CGGCTTCGAC 
GAGTACTACA AGGAGCAAGG CATTGTTCCT GAAGATGAAT GGGATGATTT CATTTCTTTG 
CTCCGAAAGC CTCTGCCTGC AGCATTTAGA ATCAATGCAA GTGGCCAATT TTTTCAGGAC 
ATTCGATCAC AGTTGGAAAA TGACTTCATG AAATCACTGC AGGCTGAGGT AACAGATGAT 
TATGAAGCGG AAGCTATTAG ACCATTGTCA TGGTATCCAG GGAACATTGC ATGGCATTTG 
AACTTTTCTC GTATGCAACT TAGGAAGAAC CAGGCACTTG AGAGGTTCCA TGAGTTTTTA 
AAGCAAGAGA ATGAGATTGG GAACATAACA AGGCAAGAGG CTGTCAGCAT GGTTCCTCCA 
CTCTTCCTTG ATGTACTACC GGATCACCAC ATTCTTGACA TGTGTGCTGC TCCAGGATCT 
AAAACTTTCC AGTTGCTTGA AATTATGCAC CAATCAATAG GACCCGGAGT ACTTCCCAAT 
GGGCTGGTGA TAGCTAATGA CGTCGATGTT CAAAGATGTA ATCTCCTCAT CCATCAAACA 
AAGAGGATGT CCACTGCCAA CTTGATAGTA ACAAATCATG AAGCCCAACA TTTTCCCAGT 
TGTTCTTTGA AAAAGGGTCA AGGAGTTGCT TCTGAAGGGC AGGAGGAGCA TGGAATACAT 
ACATTAGAGT TTGACCGTGT TTTATGTGAT GTTCCTTGTA GTGGTGATGG CACCCTGCGC 
AAGGCTCCTG ATATCTGGAG AAAATGGAAT GCAGGCATGG GAAATGGTCT TCATCGTCTC 
CAAGTGGAGA TTGCTATGCG TGGTTTAGCT TTGCTTAAAG TGGGTGGAAG GATGGTCTAC 
TCTACCTGTT CACTGAACCC AGTTGAAAAT GAAGCTGTTG TTGCTGAGGT TCTTCGAAGA 
AGTAGGGGAT CAGTTGAGCT TCTTGATGTC TCCAATGAGC TACCTGAATT GGTGCGCAGG 
CCCGGTCTTA ATACATGGAA GGTACGTGAT AGAGGAGTTT GGCTAACCTC TTATAAGGAT 
GTTCCCCTCT ATAGGCGAAG TGTAATATTA CCAAGCATGT TTCCTTCTGG TCAAAGCTGC 
AAAGAAAACC ATACACTATG CAATGATGTC CAAAAGGATG TAAAAATAGT TGGTAATAAC 
TTAGGGGAGG AAACTGTAGG AGTAGTAGTT ATAGAACAGG ATCAGACAAA TACGGAGCAT 
CAAAATTCTT CTGATGATTT AAGCAGCAAT ACCATTCCAT TGGACACTGT CAAAGCTGCA 
GCTATGGCCA ATGAAGTCTC CAAAAAAAGT ATACCTCCTG AGGAATTGGA TAAGAATTCC 
ATTGCAATGG AAGTTTCAGG TTTTCCACTC CATTATTGCA TGAGAATAGT TCCTCATGAT 
CAAAACAGCG GAGCATTTTT TATTGCTGTT CTTCAAAAGA TGGCCCCTTT GCATGTTCCG 
GAGAATCATG CAAGTGAGCC AGCTAAATTT AGTCAGGTAT CAGAAAATAC CAGTGAGAAC 
CCCGAGGATG AAAATGAAGC AGAAGAAAGG ATATCTGGAC AAGTCTCAAT TCAAGCAGAA 
GAAAATACTG ACACGAATGT TCTAGAGGAG CAACTGAAAT CAAATTTAAG TACTAAAAAT 
GCTTGCTCTA CAGCTAATAA CAAAGAGGAA GTGGTTAAGT GTGACGGTGG GAAGAGTGGT 
CACATGGGGA TTAAGGATGT AGAGAGGAAA GGCAAGTGGC GAGGGGTTGA TCCTGTTGTT 
TTTTTCACAG ATGAAAAAAC CATTAACAGC ATACTTTCTT TCTATGGTAT CAATGATGCA 
TTTCCACTCT ATGGTCACCT TGTGACTCGA AATAGTGATG CTAATCATGT GAAGAGAATA 
TACTACATAT CAAATTCGGT TCAGGAAGTC CTACAGCTGA ATGATCAAGT TGGTCAGCGG 
CTTAAGATTA CTTCCCTTGG TTTGAAGATT TTTGAGAGAC AAACATCAAA GGATGGATCA 
ACATGTGCAT TCCGGTTATC ATCAGAAGGA TTACCGTTGC TACTTCCATA TATCAGCAAG 
CAGATTTTGT TTGCATCTTT AGCGGACTTT CAGCACCTTT TACAGTACAG GACGATCAAA 
TTTCCAGATT TTGTTGATTC TAAATTTGGC GAAGAAGCGT CAGACTTAGT TCCTGGTTGC 
TGTGTTGTAG TGCTACGTGA AGGAAATCGG AGTACAGATT CTACTGCAGT GGATGCCTCT 
ACAGTCGCCA TTGTTTGCTG GAAAGGCAAG ACAAATATTA GTATCATGGT TTCCCCCTTG 
GACGGGAAGG AACTTCTCGA AAGACTCGCG TTTCGGTTCG GAATTAAGAG CATCTCACTA 
GAAGAGAATA AAAAATCAAA CATGGAAATT GAGGGGGTAA GCAACTTGGA GGATGTTGAA 
CCTATTGCGA CCATCGAGAC AGATAACCAA GAAATCGAGC ATTAA