LOC109710930 cDNA ORF clone, Ananas comosus(pineapple)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC109710930
Entrez Gene ID	109710930
Full Name	fruit bromelain-like
Synonyms	bromelain
Gene Type	protein-coding
Organism	Ananas comosus(pineapple)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020233743.1	XP_020089332.1	fruit bromelain-like

Pathways from BioSystems
Gene Ontology

The following LOC109710930 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC109710930 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAn65694	XM_020233743.1 Latest version!	Ananas comosus fruit bromelain-like (LOC109710930), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC109710930 ( XM_020233743.1 ) cDNA ORF clone, Ananas comosus(pineapple) -> XP_020089332.1 Ananas comosus fruit bromelain-like (LOC109710930), mRNA.

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CloneID	OAn65694
Clone ID Related Accession (Same CDS sequence)	XM_020233743.1
Accession Version	XM_020233743.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-14
Organism	Ananas comosus(pineapple)
Product	fruit bromelain-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033625.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ananas comosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCCA AAGTTCAACT CGTGTTTCTT TTCTTGTTTC TCTGTGTGAT GTGGGCTTCG 
CCATCGGCAG CTTCTCGTGA CGAACCCAGT GATCCCATGA TGAAGCGGTT TGAAGAATGG 
ATGGCAGAGT ACGGCCGAGT GTACAAGGAC AACGACGAGA AGATGCGCCG GTTTCAGATA 
TTCAAGAACA ACGTGAATCA TATCGAAACC TTTAACAATC GCAACGGAAA TTCATACACT 
CTCGGTATCA ATAAGTTTAC CGATATGACA AATAACGAAT TTGTTACTCA ATATACTGGT 
GTATCTCTCC CACTAAATTT CAAGAGAGAG CCAGTGGTGT CATTTGATGA CGTAAACATC 
TCTGCGGTGG GTCAAAGTAT TGATTGGAGA GACTATGGTG CCGTAACAGA GGTCAAAGAC 
CAAAACCCCT GTGGTTCTTG CTGGGCATTC AGTGCAATTG CGACGGTGGA AGGAATCTAC 
AAGATCGTAA CAGGGTACTT AGTATCTCTA TCGGAGCAAG AAGTTCTCGA TTGTGCTGTT 
AGCAATGGGT GCGACGGCGG CTTTGTGGAC AATGCCTACG ATTTCATCAT ATCTAACAAC 
GGTGTGGCCT CCGAAGCTGA CTATCCTTAT CAAGCATACG AAGGCGATTG CACCGCCAAT 
AGCTGGCCCA ATTCAGCTTA CATTACTGGT TATTCATATG TGCGAAGCAA CGACGAAAGC 
AGCATGAAGT ACGCTGTGTG GAATCAACCA ATAGCTGCTG CTATCGATGC CAGTGGAGAC 
AACTTTCAAT ATTACAATGG CGGTGTGTTT AGTGGACCTT GTGGAACTAG TCTCAATCAT 
GCCATCACCA TTATAGGTTA CGGGCAGGAT AGCAGTGGAA CACAATATTG GATTGTAAAG 
AACTCATGGG GTAGCTCATG GGGTGAACGT GGATACGTCC GTATGGCGAG AGGTGTGTCT 
TCGTCTGGAT TATGTGGAAT CGCCATGGAT CCTCTCTATC CCACTCTACA ATCAGGGGCT 
AATGTCGCAG TTATTAAGAT GGTTTCTGAA ACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020089332.1
CDS	71..1126
Translation	MASKVQLVFLFLFLCVMWASPSAASRDEPSDPMMKRFEEWMAEYGRVYKDNDEKMRRFQIFKNNVNHIETFNNRNGNSYTLGINKFTDMTNNEFVTQYTGVSLPLNFKREPVVSFDDVNISAVGQSIDWRDYGAVTEVKDQNPCGSCWAFSAIATVEGIYKIVTGYLVSLSEQEVLDCAVSNGCDGGFVDNAYDFIISNNGVASEADYPYQAYEGDCTANSWPNSAYITGYSYVRSNDESSMKYAVWNQPIAAAIDASGDNFQYYNGGVFSGPCGTSLNHAITIIGYGQDSSGTQYWIVKNSWGSSWGERGYVRMARGVSSSGLCGIAMDPLYPTLQSGANVAVIKMVSET

Target ORF information:

RefSeq Version	XM_020233743.1
Organism	Ananas comosus(pineapple)
Definition	Ananas comosus fruit bromelain-like (LOC109710930), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020233743.1

ORF Insert Sequence:

ATGGCTTCCA AAGTTCAACT CGTGTTTCTT TTCTTGTTTC TCTGTGTGAT GTGGGCTTCG 
CCATCGGCAG CTTCTCGTGA CGAACCCAGT GATCCCATGA TGAAGCGGTT TGAAGAATGG 
ATGGCAGAGT ACGGCCGAGT GTACAAGGAC AACGACGAGA AGATGCGCCG GTTTCAGATA 
TTCAAGAACA ACGTGAATCA TATCGAAACC TTTAACAATC GCAACGGAAA TTCATACACT 
CTCGGTATCA ATAAGTTTAC CGATATGACA AATAACGAAT TTGTTACTCA ATATACTGGT 
GTATCTCTCC CACTAAATTT CAAGAGAGAG CCAGTGGTGT CATTTGATGA CGTAAACATC 
TCTGCGGTGG GTCAAAGTAT TGATTGGAGA GACTATGGTG CCGTAACAGA GGTCAAAGAC 
CAAAACCCCT GTGGTTCTTG CTGGGCATTC AGTGCAATTG CGACGGTGGA AGGAATCTAC 
AAGATCGTAA CAGGGTACTT AGTATCTCTA TCGGAGCAAG AAGTTCTCGA TTGTGCTGTT 
AGCAATGGGT GCGACGGCGG CTTTGTGGAC AATGCCTACG ATTTCATCAT ATCTAACAAC 
GGTGTGGCCT CCGAAGCTGA CTATCCTTAT CAAGCATACG AAGGCGATTG CACCGCCAAT 
AGCTGGCCCA ATTCAGCTTA CATTACTGGT TATTCATATG TGCGAAGCAA CGACGAAAGC 
AGCATGAAGT ACGCTGTGTG GAATCAACCA ATAGCTGCTG CTATCGATGC CAGTGGAGAC 
AACTTTCAAT ATTACAATGG CGGTGTGTTT AGTGGACCTT GTGGAACTAG TCTCAATCAT 
GCCATCACCA TTATAGGTTA CGGGCAGGAT AGCAGTGGAA CACAATATTG GATTGTAAAG 
AACTCATGGG GTAGCTCATG GGGTGAACGT GGATACGTCC GTATGGCGAG AGGTGTGTCT 
TCGTCTGGAT TATGTGGAAT CGCCATGGAT CCTCTCTATC CCACTCTACA ATCAGGGGCT 
AATGTCGCAG TTATTAAGAT GGTTTCTGAA ACTTGA