jak1 cDNA ORF clone, Amphiprion ocellaris(clown anemonefish)

Gene
Clones
gRNAs

Summary

Gene Symbol	jak1
Entrez Gene ID	111582047
Full Name	Janus kinase 1
Gene Type	protein-coding
Organism	Amphiprion ocellaris(clown anemonefish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023290516.1	XP_023146284.1	tyrosine-protein kinase JAK1

Pathways from BioSystems
Gene Ontology

The following jak1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the jak1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAm93778	XM_023290516.1 Latest version!	Amphiprion ocellaris Janus kinase 1 (jak1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

jak1 ( XM_023290516.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023146284.1 Amphiprion ocellaris Janus kinase 1 (jak1), mRNA.

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CloneID	OAm93778
Clone ID Related Accession (Same CDS sequence)	XM_023290516.1
Accession Version	XM_023290516.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	tyrosine-protein kinase JAK1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019373633.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCATCTC TGTGGGTGAT GGAGTTGAGC AGGCAACTCT GTTTGAAAAT GAGGAGAAAC 
AGGAGGGCCC AACTCTCCTC CTCTCCTGCT TCCTCGTGGG GCCTGGAGAT CCACTTTTAC 
ATACCGGAGA TGCACCAGCT GGAGTACTTT AAAGGCTGCT ACACTGCTGA GGAGCTCTGT 
GTTGATGCTG CCAAGAAATG CTCAATTTCT CCCTTGTGCC ATAACCTCTT TGCCCTGTAC 
AATGAGACAA CTGACACTTG GTATCCCCCC AACTATGAGT TCAAGATCAC AGAAGAAACC 
AGTACCAAGC TGCACTATCG CATGAGGTTT TATTTCAGAA ACTGGCATGG CACCACTGAA 
GGAGAGTCCC CAGTTTGGAG ACACTGCATC AGTAAACTTA GAGGAGGACA CAGCCCACAG 
AAAACACCAG AGGGAACACC CCTACTAGAT TCTGCTTCCC TAGATTACCT GTTTGCCCAG 
GGCCAGCATG ACTTTCAGAC AGGCATGGTT CCACTGAGGA TGTCCCAGTC AGAGGCAGAG 
CAACACGAGA TAGAAAATGA GTGTCTGGGC ATGGCTGTAC TTGCGATCAC TCATTTTGCC 
ATGGATGTGG AGATGCCCCT ATCCACTGCT TCACGTGAAA TCAGCTACAA GCGTTTCATC 
CCAGAGTCGC TGAACCGCAA CATTAAGCAG CGGAACATTT TGACTCGCAT CCGCATCAAC 
AATGTGTTCA AGAAATTCCT CAGTGAATTC AACAGCCGCA CAGTCCAGGA CAGCAACATA 
ACACCGTACG ACCTGAAGAT CAAGTACTTG GCCACACTGG AGGGCTTGAC CAACAGCCTG 
GGCAGCGAAA TTCTTGAGCC GGTCTCACTC AGTATAATGC AGGAGGGGGA GCTCTGCAAT 
GGAGGTTACT TTGGGTACTA CAACCAGAGC CAAGAGCAGG GCCAGACTCA GAACATGGAG 
ACAAGCCATG ACATGCAGGT CCTGGTTACT GGTACCACTG GCATTTCCTG GAGGAAGAAA 
CCCGCTATGA CACCTGTGAT CTCCAAAGAA AAGGGAAAGT CAAAGAAGAA CAAGCAGGAT 
GGAAAACTGA AAAATGACAG AAATAAAGAG GCAAACGAAG GCTGGGTGCT GCTCTGTGAC 
TTCCATGAGA TCACACACGT TGTTGTCAGA GAGGCAACAG TCACCATCCT TACACAGGAC 
AACAAGAAGA TGGAGATGCA AATGAGTTCT CGGGCTCAGG CTCTGTCTTT TGCAGCCCTT 
GTGGACGGGT ACTTCAGGCT GACAGTCGAT GCCCACCACT ACCTGTGCAA GGAGGTAGCC 
CCAGCTTCAG TGGTGCGCAA CACCAACAAC GGTTGCCACG GACCCATCTG TACGGAGTAC 
GCCATCCAGA AGCTGCGTCA GGAGGGTAAT GAGGAGGGTG CCTACATCCT ACGCTGGAGC 
TGTACTGATT ACCAGTACAT CATCATCACT GTGGTCTGCA ATGAGATCTT GACAGACTTG 
AGGGATTCTC GTCCTGTGCG CCAGTATAAG AACTTCCAGA TTGAGGTTAC CTCCGATGCG 
TTCCGTCTGC ACGGCACAGA CACTTCGCGG TCTACTCTCA GGGAGCTGCT GGGGCATCTG 
GAAGGCCAAA ATCTCCGTAC CGACAACCTC CAGTTCCAAC TGCTCCGCTG CTGCCCCCCA 
CAGCCAAGAG AAATTTCTAA CCTGCTGGTG GTCACTAAGG ATAGAGCACC AGTCCCTCAG 
ACACCTATGC AGGACAGCCA ACTTAGCTTC CACCGCATTC TCAAGGAGGA GATTGAACAG 
GAGGAGCACC TCGGCCTGGG AACTAAAACC AACATCTACA AAGGCACACT GAGGGTGAAG 
AGTGAGGAAG ACGAGGATGC AGGTTACTCG TCCTTCCAGG AAGTCAAAGT TGTCCTGAAA 
GTGCTGGGTT CTGGACACAG GGACATCTCC TTGGCCTTCT TTGAAACAGC CAGTATGATG 
CGACAAGTGT CCCATAAACA CATCGTTCTC CTCTATGGAG TGTGTGTCCG CCACCTGGAG 
AATATCATGG TGGAGGAGTT TGTCCAACCA GGACCACTGG ACTTGTTTAT GAGGAGACAG 
CAGAGTCCAC TCAGCACACC ATGGAAGTTC CAGGTGGCCA AACAGCTGGC CTCAGCTCTC 
AGCTACTTGG AGGACAAAAA GCTGATCCAT GGCTTTGTGT GTGCTAAAAA CGTCCTGCTC 
GCCAGGGATG GACTGGGTGC AGATGAAGGA GGGCCCTTCA TCAAGCTCAG CGACCGAGGA 
ATACCAATCA CAGTACTCAC CAGGGAGGAG TGTGTGCATC GTATCCCATG GATTGCTCCA 
GAATGTGTGA AGAGTGTGTC CGCTCTGAGC GTCGCAGCTG ACAAATGGGG ATTTGGTACC 
ACCCTGTGGG AGATCTGCTA TGATGGAGAG ACTCCCCTCA AAGACAAGAA ACTCACAGAG 
AAGGAGAGGT TTTATGAAAC CGAGTGCCAA CTGGTCACGC CAGACTGCAA AGAGCTTGCT 
GAACTCATGA CCCACTGCAT GAACTATGAC CCCAAGAAGA GACCTTTCTT CAGAGCTATC 
GTCAGAGACA TAGACATGCT AGAAGAAACA AACCCATCTA TCAAACCCCA GCCTACAACA 
GAGGTGGACC CAACTGTGTT TGAAAAGAGA TTCCTGAGGA AGATCAGAGA ACTGGGAGAG 
GGTCACTTTG GTAAGGTGGA GTTGTGCCGC TATGATCCAC GGGGAGACAG AACAGGCGAG 
CTGGTGGCAG TGAAGTCGCT TAAGCCTGAG AACCAGGAGG AACAGAGCAG CAACCTCTCC 
AGTGAGATTG ACATCTTGAA GGAACTCTAC CATGAGAATA TTGTCAAATA CAAGGGTATC 
TGTCAAGAGG AAGGTGGTCA GGCTATTAAG CTGATCATGG AGTACCTTCC AATGGGCAGT 
TTGAAGGAGT ATCTACCCAG AAACAAAAGC AAGACCGACC TCAGCACCCT TCTCAGCTAC 
TCTGTTCAGA TATGCAGGGG GATGGACTAT TTGGGATCTC GACATTACAT CCACCGGGAC 
CTGGCTGCCC GAAATGTGCT AGTGGAGAAT GAGAGGACAG TGAAGATTGG AGACTTCGGT 
CTCACCAAGA CCATCAAGGA TAATGAGGGA TATTACACTG TCAAGGACGA CCACGACAGC 
CCTGTTTTCT GGTATGCTCC AGAATGTCTG ACTCTGTGTA AGTTTTACCT TGCTTCAGAC 
GTTTGGTCCT TTGGGGTGAC AATGTACGAA CTCATCACCT ACTGCGACTC CTCCAAGAGC 
CCAATGGCGC GCTATCTCGA TATGATTGGT CGAACTCAAG GTCAGATGAC AGTCATCCGA 
TTGGTGAAGG TGTTGCAAGA TGGAAGGAGG TTGCCACGCC CTGAGGGCTG TTCTGAGCAT 
GTGTACGAGC TGATGCGCAG ATGCTGGGAG CAGGCGCCTG AGAGGAGAAT CACCTTCAAG 
CGTCTGATCG AGGAGCTGAC CACCATGCAG CAGCAGCTCC AATCCATAAA CAACATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023146284.1
CDS	251..3790
Translation	MPSLWVMELSRQLCLKMRRNRRAQLSSSPASSWGLEIHFYIPEMHQLEYFKGCYTAEELCVDAAKKCSISPLCHNLFALYNETTDTWYPPNYEFKITEETSTKLHYRMRFYFRNWHGTTEGESPVWRHCISKLRGGHSPQKTPEGTPLLDSASLDYLFAQGQHDFQTGMVPLRMSQSEAEQHEIENECLGMAVLAITHFAMDVEMPLSTASREISYKRFIPESLNRNIKQRNILTRIRINNVFKKFLSEFNSRTVQDSNITPYDLKIKYLATLEGLTNSLGSEILEPVSLSIMQEGELCNGGYFGYYNQSQEQGQTQNMETSHDMQVLVTGTTGISWRKKPAMTPVISKEKGKSKKNKQDGKLKNDRNKEANEGWVLLCDFHEITHVVVREATVTILTQDNKKMEMQMSSRAQALSFAALVDGYFRLTVDAHHYLCKEVAPASVVRNTNNGCHGPICTEYAIQKLRQEGNEEGAYILRWSCTDYQYIIITVVCNEILTDLRDSRPVRQYKNFQIEVTSDAFRLHGTDTSRSTLRELLGHLEGQNLRTDNLQFQLLRCCPPQPREISNLLVVTKDRAPVPQTPMQDSQLSFHRILKEEIEQEEHLGLGTKTNIYKGTLRVKSEEDEDAGYSSFQEVKVVLKVLGSGHRDISLAFFETASMMRQVSHKHIVLLYGVCVRHLENIMVEEFVQPGPLDLFMRRQQSPLSTPWKFQVAKQLASALSYLEDKKLIHGFVCAKNVLLARDGLGADEGGPFIKLSDRGIPITVLTREECVHRIPWIAPECVKSVSALSVAADKWGFGTTLWEICYDGETPLKDKKLTEKERFYETECQLVTPDCKELAELMTHCMNYDPKKRPFFRAIVRDIDMLEETNPSIKPQPTTEVDPTVFEKRFLRKIRELGEGHFGKVELCRYDPRGDRTGELVAVKSLKPENQEEQSSNLSSEIDILKELYHENIVKYKGICQEEGGQAIKLIMEYLPMGSLKEYLPRNKSKTDLSTLLSYSVQICRGMDYLGSRHYIHRDLAARNVLVENERTVKIGDFGLTKTIKDNEGYYTVKDDHDSPVFWYAPECLTLCKFYLASDVWSFGVTMYELITYCDSSKSPMARYLDMIGRTQGQMTVIRLVKVLQDGRRLPRPEGCSEHVYELMRRCWEQAPERRITFKRLIEELTTMQQQLQSINNI

Target ORF information:

RefSeq Version	XM_023290516.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris Janus kinase 1 (jak1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023290516.1

ORF Insert Sequence:

ATGCCATCTC TGTGGGTGAT GGAGTTGAGC AGGCAACTCT GTTTGAAAAT GAGGAGAAAC 
AGGAGGGCCC AACTCTCCTC CTCTCCTGCT TCCTCGTGGG GCCTGGAGAT CCACTTTTAC 
ATACCGGAGA TGCACCAGCT GGAGTACTTT AAAGGCTGCT ACACTGCTGA GGAGCTCTGT 
GTTGATGCTG CCAAGAAATG CTCAATTTCT CCCTTGTGCC ATAACCTCTT TGCCCTGTAC 
AATGAGACAA CTGACACTTG GTATCCCCCC AACTATGAGT TCAAGATCAC AGAAGAAACC 
AGTACCAAGC TGCACTATCG CATGAGGTTT TATTTCAGAA ACTGGCATGG CACCACTGAA 
GGAGAGTCCC CAGTTTGGAG ACACTGCATC AGTAAACTTA GAGGAGGACA CAGCCCACAG 
AAAACACCAG AGGGAACACC CCTACTAGAT TCTGCTTCCC TAGATTACCT GTTTGCCCAG 
GGCCAGCATG ACTTTCAGAC AGGCATGGTT CCACTGAGGA TGTCCCAGTC AGAGGCAGAG 
CAACACGAGA TAGAAAATGA GTGTCTGGGC ATGGCTGTAC TTGCGATCAC TCATTTTGCC 
ATGGATGTGG AGATGCCCCT ATCCACTGCT TCACGTGAAA TCAGCTACAA GCGTTTCATC 
CCAGAGTCGC TGAACCGCAA CATTAAGCAG CGGAACATTT TGACTCGCAT CCGCATCAAC 
AATGTGTTCA AGAAATTCCT CAGTGAATTC AACAGCCGCA CAGTCCAGGA CAGCAACATA 
ACACCGTACG ACCTGAAGAT CAAGTACTTG GCCACACTGG AGGGCTTGAC CAACAGCCTG 
GGCAGCGAAA TTCTTGAGCC GGTCTCACTC AGTATAATGC AGGAGGGGGA GCTCTGCAAT 
GGAGGTTACT TTGGGTACTA CAACCAGAGC CAAGAGCAGG GCCAGACTCA GAACATGGAG 
ACAAGCCATG ACATGCAGGT CCTGGTTACT GGTACCACTG GCATTTCCTG GAGGAAGAAA 
CCCGCTATGA CACCTGTGAT CTCCAAAGAA AAGGGAAAGT CAAAGAAGAA CAAGCAGGAT 
GGAAAACTGA AAAATGACAG AAATAAAGAG GCAAACGAAG GCTGGGTGCT GCTCTGTGAC 
TTCCATGAGA TCACACACGT TGTTGTCAGA GAGGCAACAG TCACCATCCT TACACAGGAC 
AACAAGAAGA TGGAGATGCA AATGAGTTCT CGGGCTCAGG CTCTGTCTTT TGCAGCCCTT 
GTGGACGGGT ACTTCAGGCT GACAGTCGAT GCCCACCACT ACCTGTGCAA GGAGGTAGCC 
CCAGCTTCAG TGGTGCGCAA CACCAACAAC GGTTGCCACG GACCCATCTG TACGGAGTAC 
GCCATCCAGA AGCTGCGTCA GGAGGGTAAT GAGGAGGGTG CCTACATCCT ACGCTGGAGC 
TGTACTGATT ACCAGTACAT CATCATCACT GTGGTCTGCA ATGAGATCTT GACAGACTTG 
AGGGATTCTC GTCCTGTGCG CCAGTATAAG AACTTCCAGA TTGAGGTTAC CTCCGATGCG 
TTCCGTCTGC ACGGCACAGA CACTTCGCGG TCTACTCTCA GGGAGCTGCT GGGGCATCTG 
GAAGGCCAAA ATCTCCGTAC CGACAACCTC CAGTTCCAAC TGCTCCGCTG CTGCCCCCCA 
CAGCCAAGAG AAATTTCTAA CCTGCTGGTG GTCACTAAGG ATAGAGCACC AGTCCCTCAG 
ACACCTATGC AGGACAGCCA ACTTAGCTTC CACCGCATTC TCAAGGAGGA GATTGAACAG 
GAGGAGCACC TCGGCCTGGG AACTAAAACC AACATCTACA AAGGCACACT GAGGGTGAAG 
AGTGAGGAAG ACGAGGATGC AGGTTACTCG TCCTTCCAGG AAGTCAAAGT TGTCCTGAAA 
GTGCTGGGTT CTGGACACAG GGACATCTCC TTGGCCTTCT TTGAAACAGC CAGTATGATG 
CGACAAGTGT CCCATAAACA CATCGTTCTC CTCTATGGAG TGTGTGTCCG CCACCTGGAG 
AATATCATGG TGGAGGAGTT TGTCCAACCA GGACCACTGG ACTTGTTTAT GAGGAGACAG 
CAGAGTCCAC TCAGCACACC ATGGAAGTTC CAGGTGGCCA AACAGCTGGC CTCAGCTCTC 
AGCTACTTGG AGGACAAAAA GCTGATCCAT GGCTTTGTGT GTGCTAAAAA CGTCCTGCTC 
GCCAGGGATG GACTGGGTGC AGATGAAGGA GGGCCCTTCA TCAAGCTCAG CGACCGAGGA 
ATACCAATCA CAGTACTCAC CAGGGAGGAG TGTGTGCATC GTATCCCATG GATTGCTCCA 
GAATGTGTGA AGAGTGTGTC CGCTCTGAGC GTCGCAGCTG ACAAATGGGG ATTTGGTACC 
ACCCTGTGGG AGATCTGCTA TGATGGAGAG ACTCCCCTCA AAGACAAGAA ACTCACAGAG 
AAGGAGAGGT TTTATGAAAC CGAGTGCCAA CTGGTCACGC CAGACTGCAA AGAGCTTGCT 
GAACTCATGA CCCACTGCAT GAACTATGAC CCCAAGAAGA GACCTTTCTT CAGAGCTATC 
GTCAGAGACA TAGACATGCT AGAAGAAACA AACCCATCTA TCAAACCCCA GCCTACAACA 
GAGGTGGACC CAACTGTGTT TGAAAAGAGA TTCCTGAGGA AGATCAGAGA ACTGGGAGAG 
GGTCACTTTG GTAAGGTGGA GTTGTGCCGC TATGATCCAC GGGGAGACAG AACAGGCGAG 
CTGGTGGCAG TGAAGTCGCT TAAGCCTGAG AACCAGGAGG AACAGAGCAG CAACCTCTCC 
AGTGAGATTG ACATCTTGAA GGAACTCTAC CATGAGAATA TTGTCAAATA CAAGGGTATC 
TGTCAAGAGG AAGGTGGTCA GGCTATTAAG CTGATCATGG AGTACCTTCC AATGGGCAGT 
TTGAAGGAGT ATCTACCCAG AAACAAAAGC AAGACCGACC TCAGCACCCT TCTCAGCTAC 
TCTGTTCAGA TATGCAGGGG GATGGACTAT TTGGGATCTC GACATTACAT CCACCGGGAC 
CTGGCTGCCC GAAATGTGCT AGTGGAGAAT GAGAGGACAG TGAAGATTGG AGACTTCGGT 
CTCACCAAGA CCATCAAGGA TAATGAGGGA TATTACACTG TCAAGGACGA CCACGACAGC 
CCTGTTTTCT GGTATGCTCC AGAATGTCTG ACTCTGTGTA AGTTTTACCT TGCTTCAGAC 
GTTTGGTCCT TTGGGGTGAC AATGTACGAA CTCATCACCT ACTGCGACTC CTCCAAGAGC 
CCAATGGCGC GCTATCTCGA TATGATTGGT CGAACTCAAG GTCAGATGAC AGTCATCCGA 
TTGGTGAAGG TGTTGCAAGA TGGAAGGAGG TTGCCACGCC CTGAGGGCTG TTCTGAGCAT 
GTGTACGAGC TGATGCGCAG ATGCTGGGAG CAGGCGCCTG AGAGGAGAAT CACCTTCAAG 
CGTCTGATCG AGGAGCTGAC CACCATGCAG CAGCAGCTCC AATCCATAAA CAACATTTAA