gclc cDNA ORF clone, Amphiprion ocellaris(clown anemonefish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gclc
Entrez Gene ID	111566481
Full Name	glutamate-cysteine ligase catalytic subunit
Gene Type	protein-coding
Organism	Amphiprion ocellaris(clown anemonefish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023267088.1	XP_023122856.1	glutamate--cysteine ligase catalytic subunit

Pathways from BioSystems
Gene Ontology

The following gclc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gclc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAm75391	XM_023267088.1 Latest version!	Amphiprion ocellaris glutamate-cysteine ligase catalytic subunit (gclc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gclc ( XM_023267088.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023122856.1 Amphiprion ocellaris glutamate-cysteine ligase catalytic subunit (gclc), mRNA.

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CloneID	OAm75391
Clone ID Related Accession (Same CDS sequence)	XM_023267088.1
Accession Version	XM_023267088.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	glutamate--cysteine ligase catalytic subunit
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019371829.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTTAC TGTCACAGGG CTCTCCGCTT AACTGGGAAG AAACCAAGAA GTATGCGGAC 
CACATCAGAA AGCACGGCAT CATCCAGTTC CTCAACATCT ACAACAAGGT GAAGGACCGC 
CAGAAAGATG TGCTGAAGTG GGGCGATGAG GTTGAGTACA TGTTGTTGGA GCTGGACGAC 
AAGGATGAGA AGGTTCGGAT CGTCCTGAAT GGTGGAGAAG TTCTAGAAAC TCTCCAGGAC 
CAGGGTGAAA AGATAAACCC CAATCACCCC ACGCTTTGGA GACCAGAATA TGGCAGCTAC 
ATGATTGAAG GAACTCCAGG GCAGCCGTAC GGAGGGACGA TGTCAGAGTT TAACACTGTA 
GAGGGCAACA TGGGGAAGAG ACGACGAGAG GCGTCATCTG TCCTGAATCA GAATGAAACC 
CTGTGCACCA TCACCTCATT TCCAAGGTTA GGTTGCCCAG GCTTCACCAA ACCAGAGTAC 
CGGCCGACCC CTGTTGAAAA GGGAGTGTCC AAGTCATTGT TCTTCCCAGA TGAAGCCATC 
AACAGACACC CAAGATTCAG CACCCTTACC AGAAACATAC GTCACAGAAG AGGAGAGAAA 
GTTGCAATAA ATGTGCCAAT CTTCAAAGAC AAGTGCACTC CATCTCCATT TGTGGAGGAG 
TTTCCTGAGG ACGACGGCGA AGCAGCGAGA GCGGCTCTGC CCGATCACAT CTACATGGAT 
GCTATGGGCT TTGGCATGGG CAACTGCTGC CTGCAGGTGA CATTCCAGGC TTGCAGCATT 
GATGAGGCAA GGTATCTTTA TGACCAACTA GCAACATTCT GCCCCATTGT GATGGCGCTG 
AGTGCAGCCT CACCGTTCTA CAGAGGCTAC GTGTCAGATA ACGATTGTCG CTGGGGAGTT 
ATTTCTGCCT CGGTGGATGA CAGAACACAA GAGGAACGTG GGCTGAAGCC TTTGAAAAAC 
AACAAATACA GGATCTTCAA GTCAAGATAT GACTCAATTG ACAGCTACCT GTCTAACTGC 
GGTGAGAAGT ACAATGACAT TGATTTGACA ATAGACGAGG AGATATACCA GCAGCTGCTC 
GATGCAGGCA TTGACAAGCT TCTGGCCCAA CACATAGCAC ATCTCTTCAT CCGAGACCCG 
CTCTCTGTCT TCGAGGAGAA GATACACTTG GATGATGAAA ATGAGTCGGA TCACTTTGAG 
AACCTCCAAT CGACCAACTG GCAGACAATG AGGTTTAAAC CTCCACCTCC AAACTCGGAC 
ATCGGCTGGA GAGTAGAGTT CCGACCCATG GAGGTGCAAC TAACTGACTT TGAAAACGCT 
GCGTACGTGG TCTTTGTCGT CCTGCTCACC AGAGTGATCT TGTCCTACAA GTTGGACTTC 
CTGATCCCGT TGTCAAAGGT TGATGAAAAC ATGAAGGTTG CACAAAAGCG AAATGCAGTC 
CAGGAGGGAA TGTTTTACTT CAGAAAGGAC ATCTTTAAAG GCTGCAACCC GGTCCTTGAT 
GGCACTGCTT CTGCTCAGAA CGGCTTGGAG ACTGACTGTG GCAATGAGGA GTACACACTG 
ATGAGCATCG ACACCATCAT TAATGGAAAG GAGGGAGTTT TTCAAGGGCT CATCCCGATC 
CTGAACTGCT ATTTGGAAAA CATGGAGGTT GATGTGGACA CCAGGTGCAC CATTTTGAAT 
TATCTGAAGC TCATCAAGAA ACGTGCCTCA GGCGAACTGA TGACCATGGC CAAGTGGATG 
AGGGAGTTTG TTGCAAATCA CCCACAGTAC AAGCAGGACA GCGTCATCAC TGACAAGATC 
AACTATGACC TGTTCAGAAA GTGTGACAGG GTTGCAAAAG GTGAAGAGCA GTGCCCCGAG 
CTCATTGGAA ACCCAGTGAA CAAGTTCAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023122856.1
CDS	515..2407
Translation	MGLLSQGSPLNWEETKKYADHIRKHGIIQFLNIYNKVKDRQKDVLKWGDEVEYMLLELDDKDEKVRIVLNGGEVLETLQDQGEKINPNHPTLWRPEYGSYMIEGTPGQPYGGTMSEFNTVEGNMGKRRREASSVLNQNETLCTITSFPRLGCPGFTKPEYRPTPVEKGVSKSLFFPDEAINRHPRFSTLTRNIRHRRGEKVAINVPIFKDKCTPSPFVEEFPEDDGEAARAALPDHIYMDAMGFGMGNCCLQVTFQACSIDEARYLYDQLATFCPIVMALSAASPFYRGYVSDNDCRWGVISASVDDRTQEERGLKPLKNNKYRIFKSRYDSIDSYLSNCGEKYNDIDLTIDEEIYQQLLDAGIDKLLAQHIAHLFIRDPLSVFEEKIHLDDENESDHFENLQSTNWQTMRFKPPPPNSDIGWRVEFRPMEVQLTDFENAAYVVFVVLLTRVILSYKLDFLIPLSKVDENMKVAQKRNAVQEGMFYFRKDIFKGCNPVLDGTASAQNGLETDCGNEEYTLMSIDTIINGKEGVFQGLIPILNCYLENMEVDVDTRCTILNYLKLIKKRASGELMTMAKWMREFVANHPQYKQDSVITDKINYDLFRKCDRVAKGEEQCPELIGNPVNKFK

Target ORF information:

RefSeq Version	XM_023267088.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris glutamate-cysteine ligase catalytic subunit (gclc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023267088.1

ORF Insert Sequence:

ATGGGTTTAC TGTCACAGGG CTCTCCGCTT AACTGGGAAG AAACCAAGAA GTATGCGGAC 
CACATCAGAA AGCACGGCAT CATCCAGTTC CTCAACATCT ACAACAAGGT GAAGGACCGC 
CAGAAAGATG TGCTGAAGTG GGGCGATGAG GTTGAGTACA TGTTGTTGGA GCTGGACGAC 
AAGGATGAGA AGGTTCGGAT CGTCCTGAAT GGTGGAGAAG TTCTAGAAAC TCTCCAGGAC 
CAGGGTGAAA AGATAAACCC CAATCACCCC ACGCTTTGGA GACCAGAATA TGGCAGCTAC 
ATGATTGAAG GAACTCCAGG GCAGCCGTAC GGAGGGACGA TGTCAGAGTT TAACACTGTA 
GAGGGCAACA TGGGGAAGAG ACGACGAGAG GCGTCATCTG TCCTGAATCA GAATGAAACC 
CTGTGCACCA TCACCTCATT TCCAAGGTTA GGTTGCCCAG GCTTCACCAA ACCAGAGTAC 
CGGCCGACCC CTGTTGAAAA GGGAGTGTCC AAGTCATTGT TCTTCCCAGA TGAAGCCATC 
AACAGACACC CAAGATTCAG CACCCTTACC AGAAACATAC GTCACAGAAG AGGAGAGAAA 
GTTGCAATAA ATGTGCCAAT CTTCAAAGAC AAGTGCACTC CATCTCCATT TGTGGAGGAG 
TTTCCTGAGG ACGACGGCGA AGCAGCGAGA GCGGCTCTGC CCGATCACAT CTACATGGAT 
GCTATGGGCT TTGGCATGGG CAACTGCTGC CTGCAGGTGA CATTCCAGGC TTGCAGCATT 
GATGAGGCAA GGTATCTTTA TGACCAACTA GCAACATTCT GCCCCATTGT GATGGCGCTG 
AGTGCAGCCT CACCGTTCTA CAGAGGCTAC GTGTCAGATA ACGATTGTCG CTGGGGAGTT 
ATTTCTGCCT CGGTGGATGA CAGAACACAA GAGGAACGTG GGCTGAAGCC TTTGAAAAAC 
AACAAATACA GGATCTTCAA GTCAAGATAT GACTCAATTG ACAGCTACCT GTCTAACTGC 
GGTGAGAAGT ACAATGACAT TGATTTGACA ATAGACGAGG AGATATACCA GCAGCTGCTC 
GATGCAGGCA TTGACAAGCT TCTGGCCCAA CACATAGCAC ATCTCTTCAT CCGAGACCCG 
CTCTCTGTCT TCGAGGAGAA GATACACTTG GATGATGAAA ATGAGTCGGA TCACTTTGAG 
AACCTCCAAT CGACCAACTG GCAGACAATG AGGTTTAAAC CTCCACCTCC AAACTCGGAC 
ATCGGCTGGA GAGTAGAGTT CCGACCCATG GAGGTGCAAC TAACTGACTT TGAAAACGCT 
GCGTACGTGG TCTTTGTCGT CCTGCTCACC AGAGTGATCT TGTCCTACAA GTTGGACTTC 
CTGATCCCGT TGTCAAAGGT TGATGAAAAC ATGAAGGTTG CACAAAAGCG AAATGCAGTC 
CAGGAGGGAA TGTTTTACTT CAGAAAGGAC ATCTTTAAAG GCTGCAACCC GGTCCTTGAT 
GGCACTGCTT CTGCTCAGAA CGGCTTGGAG ACTGACTGTG GCAATGAGGA GTACACACTG 
ATGAGCATCG ACACCATCAT TAATGGAAAG GAGGGAGTTT TTCAAGGGCT CATCCCGATC 
CTGAACTGCT ATTTGGAAAA CATGGAGGTT GATGTGGACA CCAGGTGCAC CATTTTGAAT 
TATCTGAAGC TCATCAAGAA ACGTGCCTCA GGCGAACTGA TGACCATGGC CAAGTGGATG 
AGGGAGTTTG TTGCAAATCA CCCACAGTAC AAGCAGGACA GCGTCATCAC TGACAAGATC 
AACTATGACC TGTTCAGAAA GTGTGACAGG GTTGCAAAAG GTGAAGAGCA GTGCCCCGAG 
CTCATTGGAA ACCCAGTGAA CAAGTTCAAA TGA