UPF1 cDNA ORF clone, Alligator sinensis(Chinese alligator)

Gene
Clones
gRNAs

Summary

Gene Symbol	UPF1
Entrez Gene ID	102384608
Full Name	UPF1, RNA helicase and ATPase
Gene Type	protein-coding
Organism	Alligator sinensis(Chinese alligator)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014522341.1	XP_014377827.1	LOW QUALITY PROTEIN: regulator of nonsense transcripts 1
XM_025210325.1	XP_025066110.1	LOW QUALITY PROTEIN: regulator of nonsense transcripts 1

Pathways from BioSystems
Gene Ontology

The following UPF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UPF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAl23070	XM_014522341.1 Latest version!	Alligator sinensis UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OAl91043	XM_025210325.1 Latest version!	Alligator sinensis UPF1, RNA helicase and ATPase (UPF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UPF1 ( XM_014522341.1 ) cDNA ORF clone, Alligator sinensis(Chinese alligator) -> XP_014377827.1 Alligator sinensis UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), mRNA.

Price & Availability↑

CloneID	OAl23070
Clone ID Related Accession (Same CDS sequence)	XM_014522341.1
Accession Version	XM_014522341.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-10-29
Organism	Alligator sinensis(Chinese alligator)
Product	LOW QUALITY PROTEIN: regulator of nonsense transcripts 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005842344.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator sinensis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 4% of CDS bases frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGTTTGCAC CCAATGCAGT GGAAGGTTTT ACTCTCTATC ATCCCCCCTA CAGAGGTGCA 
GGTGCCCATC AAGATATGAC ATTGGTCAAA AGAGAGGGAG AATTCAAGAA AAAAGGCTTG 
CACCTGATGG TCAACCAGAA GGTTAATGGA CCTGATGGCA TCCTACAGAA CGGTGCCGTG 
GATGATAATG TGGCTAAAAC CAGCCAACTT CTGGCAGAGT TGAACTTTGA AGAAGATGAA 
GAAGACACCT ATTATACCAA GGATCTTCCT GTGCATGCTT GCAGTTACTG TGGCATTCAT 
GACCCAGCCT GCGTGGTTTA CTGCAACACC AGCAAAAAGT GGTTCTGCAA TGGGCGTGGC 
AATACATCCG GCAGCCACAT TGTAAACCAT CTGGTGAGAG CCAAATGCAA GGAGGTAACT 
CTCCACAAAG ACGGGCCTCT TGGAGAGACT GTCTTGGAGT GCTACAATTG TGGATGCCGG 
AACGTGTTCC TCCTTGGCTT CATTCCTGCA AAGGCTGACT CGGTAGTAGT CTTGCTGTGC 
AGGCAACCAT GTGCCAGTCA GAGCAGTTTA AAGGATATTA ATTGGGACAG TTCACAGTGG 
CAGCCTCTTA TCCAAGACCG CTGCTTCCTT TCCTGGCTGG TAAAGATTCC CTCTGAACAG 
GAGCAGCTGC GAGCCCGTCA GATCACAGCT CAGCAAATTA ACAAACTGGA GGAACTCTGG 
AAGGAAAACC CCTCTGCAAC CCTTGAAGAC TTGGAAAAAC CTGGTGTTGA TGAAGAACCG 
CAGCATGTCT TACTTCGATA TGAGGATGCT TATCAGTACC AAAATATCTT TGGACCTTTA 
GTTAAGCTGG AGGCAGACTA TGACAAGAAA CTCAAGGAAT CCCAGACCCA GGATAACATC 
ACAGTGAGAT GGGACCTAGG CTTAAACAAG AAGAGAATTG CTTACTTCAC TTTGCCAAAG 
ACTGACTCAG ATATGCGTCT TATGCAAGGA GATGAGATCT GCCTGAGGTA TAAAGGAGAC 
CTGGCCCCTC TGTGGAAAGG AATTGGTCAT GTTATCAAAG TCCCGGATAA TTATGGTGAT 
GAGATAGCCA TTGAGCTGAG AAGCAGCGTT GGAGCACCTG TGGAGGTTAC TCATAACTTC 
CAGGTGGATT TTGTTTGGAA GTCTACTTCA TTTGACAGAA TGCAAAGTGC TTTAAAAACA 
TTTGCAGTGG ATGAAACTTC TGTGTCTGGC TACATCTACC ACAAGCTGTT AGGTCATGAG 
GTAGAAGATG TGATTATTAA ATGCCAGCTG CCAAAGCGTT TTACAGCGCA AGGACTGCCT 
GACCTCAACC ATTCTCAGGT TTATGCTGTG AAGACTGTCT TGCAGCGTCC TCTGAGCCTT 
ATCCAAGGTC CCCCCGGCAC AGGAAAAACA GTGACATCAG CAACGATTGT CTACCATCTA 
GCTAGGCAGG GCAATGGGCC CGTGTTAGTT TGTGCCCCAA GTAACATAGC TGTGGATCAG 
CTGACCGAGA AGATTCATCA GACGGGACTG AAAGTGGTTC GCCTGTGTGC TAAAAGTCGT 
GAGGCAATCG ATTCTCCTGT ATCTTTCTTG GCATTGCACA ACCAGATCAG AAACATGGAC 
AGTATGCCTG AGCTTCAGAA ACTGCAGCAG CTGAAAGATG AAACTGGAGA ATTGTCATCT 
GCTGATGAGA AGCGTTATCG TGCACTGAAG CGAACAGCAG AACGTGAATT ACTTATGAAT 
GCAGATGTTA TCTGCTGCAC GTGTGTGGGA GCAGGTGACC CGAGACTTGC GAAAATGCAG 
TTCCGCTCCA TTTTAATTGA TGAAAGCACG CAGGCTACTG AACCGGAGTG TATGGTGCCC 
GTGGTTCTTG GAGCAAAGCA GCTGATCCTC GTCGGTGACC ACTGCCAGCT GGGTCCTGTT 
GTGATGTGTA AGAAAGCTGC AAAGGCAGGC CTGTCTCAGT CTCTCTTTGA GAGGCTTGTG 
GTGTTGGGGA TCCGTCCAAT CCGCCTGCAA GTGCAGTATC GGATGCACCC TGCACTTAGT 
GCTTTTCCAT CCAACATCTT CTACGAGGGC TCTCTCCAGA ACGGCGTCAC TGCAGCGGAC 
CGTGTGAAAA AGGGATTTGA TTTCCAGTGG CCCCAGCCAG ATAAGCCGAT GTTCTTCTAT 
GTGACCCAGG GGCAGGAAGA AATTGCCAGT TCAGGCACCT CTTACTTGAA CAGGACGGAA 
GCTGCTAATG TGGAGAAGAT AACTACAAAA CTGTTGAAAG CTGGTGCCAA ACCAGATCAG 
ATTGGCATTA TTACACCCTA CGAGGGCCAG CGATCTTACC TGGTGCAGTA CATGCAGTTC 
AGCGGGTCCT TGCATACAAA GCTCTACCAG GAAGTGGAAA TCGCAAGTGT CGATGCTTTC 
CAGGGACGGG AGAAGGATTT TATTATCCTT TCTTGCGTAA GAGCCAATGA GCACCAAGGA 
ATTGGATTTT TAAATGATCC CAGACGTCTT AACGTGGCTC TTACCAGAGC AAGATATGGA 
GTCATTATTG TTGGGAACCC AAAGGCTCTG NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG 
CTGAACTACT ACAAAGAGCA GAAGGTGCTG GTAGAGGGAC CACTGAACAA CCTCCGGGAA 
AGCCTCATGC AGTTCAGCAA GCCTCGTAAA CTCGTCAATA CCATCAACCC TGGTGCCCGT 
TTCATGACGA CTGCCATGTA TGACGCACGT GAAGCTATTA TCCCAGGATC TGTCTATGAC 
AGGAGCAGTC AAGGGCGCCC ATCCAACATG TACTTCCAGA CTCACGACCA GATCGGGATG 
ATTAGTGCTG GGCCCAGCCA CGTCCCTGCG ATGAACATTC CTATCCCTTT CAATCTCGTC 
ATGCCACCCA TGCCGCCCCC AGGATACTTC GGGCAGGCCA ATGGCCCGGC GGCAGGACGC 
GGCACACCCA AAGGGAAGAC TGGCCGTGGC GGGCGCCAGA AAAACCGTTT TGGGATTCCT 
GGAACTAGCC AGTCAAACCT TCCCAACAGT CAAGCGAGCC AAGATGTGGT GTCCCAGCCT 
TTCTCCCAGG GTCCACTGAC TCAGGGCTAT ATCTCCATGA GTCAGCCGTC GCAGATGAGC 
CAGCCAGGAC TCTCCCAGCC AGAATTATCA CAGGACAGTT ACCTTGGTGA TGAGTTTAAA 
TCTCAGATTG ATGTGGCGCT GTCACAGGAT TCTACCTACC AGGGTGAACG TGCATATCAA 
CACGGTGGAG TGACGGGATT GTCACAGTAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014377827.1
CDS	1..3273
Translation	MFAPNAVEGFTLYHPPYRGAGAHQDMTLVKREGEFKKKGLHLMVNQKVNGPDGILQNGAVDDNVAKTSQLLAELNFEEDEEDTYYTKDLPVHACSYCGIHDPACVVYCNTSKKWFCNGRGNTSGSHIVNHLVRAKCKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCRQPCASQSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQITAQQINKLEELWKENPSATLEDLEKPGVDEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAYFTLPKTDSDMRLMQGDEICLRYKGDLAPLWKGIGHVIKVPDNYGDEIAIELRSSVGAPVEVTHNFQVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVIIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQRPLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDSPVSFLALHNQIRNMDSMPELQKLQQLKDETGELSSADEKRYRALKRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRPIRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRVKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTKLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALnullnullnullnullnullnullnullnullnullnullLNYYKEQKVLVEGPLNNLRESLMQFSKPRKLVNTINPGARFMTTAMYDAREAIIPGSVYDRSSQGRPSNMYFQTHDQIGMISAGPSHVPAMNIPIPFNLVMPPMPPPGYFGQANGPAAGRGTPKGKTGRGGRQKNRFGIPGTSQSNLPNSQASQDVVSQPFSQGPLTQGYISMSQPSQMSQPGLSQPELSQDSYLGDEFKSQIDVALSQDSTYQGERAYQHGGVTGLSQY

Target ORF information:

RefSeq Version	XM_014522341.1
Organism	Alligator sinensis(Chinese alligator)
Definition	Alligator sinensis UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014522341.1

ORF Insert Sequence:

ATGTTTGCAC CCAATGCAGT GGAAGGTTTT ACTCTCTATC ATCCCCCCTA CAGAGGTGCA 
GGTGCCCATC AAGATATGAC ATTGGTCAAA AGAGAGGGAG AATTCAAGAA AAAAGGCTTG 
CACCTGATGG TCAACCAGAA GGTTAATGGA CCTGATGGCA TCCTACAGAA CGGTGCCGTG 
GATGATAATG TGGCTAAAAC CAGCCAACTT CTGGCAGAGT TGAACTTTGA AGAAGATGAA 
GAAGACACCT ATTATACCAA GGATCTTCCT GTGCATGCTT GCAGTTACTG TGGCATTCAT 
GACCCAGCCT GCGTGGTTTA CTGCAACACC AGCAAAAAGT GGTTCTGCAA TGGGCGTGGC 
AATACATCCG GCAGCCACAT TGTAAACCAT CTGGTGAGAG CCAAATGCAA GGAGGTAACT 
CTCCACAAAG ACGGGCCTCT TGGAGAGACT GTCTTGGAGT GCTACAATTG TGGATGCCGG 
AACGTGTTCC TCCTTGGCTT CATTCCTGCA AAGGCTGACT CGGTAGTAGT CTTGCTGTGC 
AGGCAACCAT GTGCCAGTCA GAGCAGTTTA AAGGATATTA ATTGGGACAG TTCACAGTGG 
CAGCCTCTTA TCCAAGACCG CTGCTTCCTT TCCTGGCTGG TAAAGATTCC CTCTGAACAG 
GAGCAGCTGC GAGCCCGTCA GATCACAGCT CAGCAAATTA ACAAACTGGA GGAACTCTGG 
AAGGAAAACC CCTCTGCAAC CCTTGAAGAC TTGGAAAAAC CTGGTGTTGA TGAAGAACCG 
CAGCATGTCT TACTTCGATA TGAGGATGCT TATCAGTACC AAAATATCTT TGGACCTTTA 
GTTAAGCTGG AGGCAGACTA TGACAAGAAA CTCAAGGAAT CCCAGACCCA GGATAACATC 
ACAGTGAGAT GGGACCTAGG CTTAAACAAG AAGAGAATTG CTTACTTCAC TTTGCCAAAG 
ACTGACTCAG ATATGCGTCT TATGCAAGGA GATGAGATCT GCCTGAGGTA TAAAGGAGAC 
CTGGCCCCTC TGTGGAAAGG AATTGGTCAT GTTATCAAAG TCCCGGATAA TTATGGTGAT 
GAGATAGCCA TTGAGCTGAG AAGCAGCGTT GGAGCACCTG TGGAGGTTAC TCATAACTTC 
CAGGTGGATT TTGTTTGGAA GTCTACTTCA TTTGACAGAA TGCAAAGTGC TTTAAAAACA 
TTTGCAGTGG ATGAAACTTC TGTGTCTGGC TACATCTACC ACAAGCTGTT AGGTCATGAG 
GTAGAAGATG TGATTATTAA ATGCCAGCTG CCAAAGCGTT TTACAGCGCA AGGACTGCCT 
GACCTCAACC ATTCTCAGGT TTATGCTGTG AAGACTGTCT TGCAGCGTCC TCTGAGCCTT 
ATCCAAGGTC CCCCCGGCAC AGGAAAAACA GTGACATCAG CAACGATTGT CTACCATCTA 
GCTAGGCAGG GCAATGGGCC CGTGTTAGTT TGTGCCCCAA GTAACATAGC TGTGGATCAG 
CTGACCGAGA AGATTCATCA GACGGGACTG AAAGTGGTTC GCCTGTGTGC TAAAAGTCGT 
GAGGCAATCG ATTCTCCTGT ATCTTTCTTG GCATTGCACA ACCAGATCAG AAACATGGAC 
AGTATGCCTG AGCTTCAGAA ACTGCAGCAG CTGAAAGATG AAACTGGAGA ATTGTCATCT 
GCTGATGAGA AGCGTTATCG TGCACTGAAG CGAACAGCAG AACGTGAATT ACTTATGAAT 
GCAGATGTTA TCTGCTGCAC GTGTGTGGGA GCAGGTGACC CGAGACTTGC GAAAATGCAG 
TTCCGCTCCA TTTTAATTGA TGAAAGCACG CAGGCTACTG AACCGGAGTG TATGGTGCCC 
GTGGTTCTTG GAGCAAAGCA GCTGATCCTC GTCGGTGACC ACTGCCAGCT GGGTCCTGTT 
GTGATGTGTA AGAAAGCTGC AAAGGCAGGC CTGTCTCAGT CTCTCTTTGA GAGGCTTGTG 
GTGTTGGGGA TCCGTCCAAT CCGCCTGCAA GTGCAGTATC GGATGCACCC TGCACTTAGT 
GCTTTTCCAT CCAACATCTT CTACGAGGGC TCTCTCCAGA ACGGCGTCAC TGCAGCGGAC 
CGTGTGAAAA AGGGATTTGA TTTCCAGTGG CCCCAGCCAG ATAAGCCGAT GTTCTTCTAT 
GTGACCCAGG GGCAGGAAGA AATTGCCAGT TCAGGCACCT CTTACTTGAA CAGGACGGAA 
GCTGCTAATG TGGAGAAGAT AACTACAAAA CTGTTGAAAG CTGGTGCCAA ACCAGATCAG 
ATTGGCATTA TTACACCCTA CGAGGGCCAG CGATCTTACC TGGTGCAGTA CATGCAGTTC 
AGCGGGTCCT TGCATACAAA GCTCTACCAG GAAGTGGAAA TCGCAAGTGT CGATGCTTTC 
CAGGGACGGG AGAAGGATTT TATTATCCTT TCTTGCGTAA GAGCCAATGA GCACCAAGGA 
ATTGGATTTT TAAATGATCC CAGACGTCTT AACGTGGCTC TTACCAGAGC AAGATATGGA 
GTCATTATTG TTGGGAACCC AAAGGCTCTG NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG 
CTGAACTACT ACAAAGAGCA GAAGGTGCTG GTAGAGGGAC CACTGAACAA CCTCCGGGAA 
AGCCTCATGC AGTTCAGCAA GCCTCGTAAA CTCGTCAATA CCATCAACCC TGGTGCCCGT 
TTCATGACGA CTGCCATGTA TGACGCACGT GAAGCTATTA TCCCAGGATC TGTCTATGAC 
AGGAGCAGTC AAGGGCGCCC ATCCAACATG TACTTCCAGA CTCACGACCA GATCGGGATG 
ATTAGTGCTG GGCCCAGCCA CGTCCCTGCG ATGAACATTC CTATCCCTTT CAATCTCGTC 
ATGCCACCCA TGCCGCCCCC AGGATACTTC GGGCAGGCCA ATGGCCCGGC GGCAGGACGC 
GGCACACCCA AAGGGAAGAC TGGCCGTGGC GGGCGCCAGA AAAACCGTTT TGGGATTCCT 
GGAACTAGCC AGTCAAACCT TCCCAACAGT CAAGCGAGCC AAGATGTGGT GTCCCAGCCT 
TTCTCCCAGG GTCCACTGAC TCAGGGCTAT ATCTCCATGA GTCAGCCGTC GCAGATGAGC 
CAGCCAGGAC TCTCCCAGCC AGAATTATCA CAGGACAGTT ACCTTGGTGA TGAGTTTAAA 
TCTCAGATTG ATGTGGCGCT GTCACAGGAT TCTACCTACC AGGGTGAACG TGCATATCAA 
CACGGTGGAG TGACGGGATT GTCACAGTAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UPF1 ( XM_025210325.1 ) cDNA ORF clone, Alligator sinensis(Chinese alligator) -> XP_025066110.1 Alligator sinensis UPF1, RNA helicase and ATPase (UPF1), mRNA.

Price & Availability↑

CloneID	OAl91043
Clone ID Related Accession (Same CDS sequence)	XM_025210325.1
Accession Version	XM_025210325.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Alligator sinensis(Chinese alligator)
Product	LOW QUALITY PROTEIN: regulator of nonsense transcripts 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005842344.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Alligator sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGAATACA GTTTTGTGGT GCAGGGTGTT AAAGTTAATG GACCTGATGG CATCCTACAG 
AACGGTGCCG TGGATGATAA TGTGGCTAAA ACCAGCCAAC TTCTGGCAGA GTTGAACTTT 
GAAGAAGATG AAGAAGACAC CTATTATACC AAGGATCTTC CTGTGCATGC TTGCAGTTAC 
TGTGGCATTC ATGACCCAGC CTGCGTGGTT TACTGCAACA CCAGCAAAAA GTGGTTCTGC 
AATGGGCGTG GCAATACATC CGGCAGCCAC ATTGTAAACC ATCTGGTGAG AGCCAAATGC 
AAGGAGGTAA CTCTCCACAA AGACGGGCCT CTTGGAGAGA CTGTCTTGGA GTGCTACAAT 
TGTGGATGCC GGAACGTGTT CCTCCTTGGC TTCATTCCTG CAAAGGCTGA CTCGGTAGTA 
GTCTTGCTGT GCAGGCAACC ATGTGCCAGT CAGAGCAGTT TAAAGGATAT TAATTGGGAC 
AGTTCACAGT GGCAGCCTCT TATCCAAGAC CGCTGCTTCC TTTCCTGGCT GGTAAAGATT 
CCCTCTGAAC AGGAGCAGCT GCGAGCCCGT CAGATCACAG CTCAGCAAAT TAACAAACTG 
GAGGAACTCT GGAAGGAAAA CCCCTCTGCA ACCCTTGAAG ACTTGGAAAA ACCTGGTGTT 
GATGAAGAAC CGCAGCATGT CTTACTTCGA TATGAGGATG CTTATCAGTA CCAAAATATC 
TTTGGACCTT TAGTTAAGCT GGAGGCAGAC TATGACAAGA AACTCAAGGA ATCCCAGACC 
CAGGATAACA TCACAGTGAG ATGGGACCTA GGCTTAAACA AGAAGAGAAT TGCTTACTTC 
ACTTTGCCAA AGACTGACTC AGATATGCGT CTTATGCAAG GAGATGAGAT CTGCCTGAGG 
TATAAAGGAG ACCTGGCCCC TCTGTGGAAA GGAATTGGTC ATGTTATCAA AGTCCCGGAT 
AATTATGGTG ATGAGATAGC CATTGAGCTG AGAAGCAGCG TTGGAGCACC TGTGGAGGTT 
ACTCATAACT TCCAGGTGGA TTTTGTTTGG AAGTCTACTT CATTTGACAG AATGCAAAGT 
GCTTTAAAAA CATTTGCAGT GGATGAAACT TCTGTGTCTG GCTACATCTA CCACAAGCTG 
TTAGGTCATG AGGTAGAAGA TGTGATTATT AAATGCCAGC TGCCAAAGCG TTTTACAGCG 
CAAGGACTGC CTGACCTCAA CCATTCTCAG GTTTATGCTG TGAAGACTGT CTTGCAGCGT 
CCTCTGAGCC TTATCCAAGG TCCCCCCGGC ACAGGAAAAA CAGTGACATC AGCAACGATT 
GTCTACCATC TAGCTAGGCA GGGCAATGGG CCCGTGTTAG TTTGTGCCCC AAGTAACATA 
GCTGTGGATC AGCTGACCGA GAAGATTCAT CAGACGGGAC TGAAAGTGGT TCGCCTGTGT 
GCTAAAAGTC GTGAGGCAAT CGATTCTCCT GTATCTTTCT TGGCATTGCA CAACCAGATC 
AGAAACATGG ACAGTATGCC TGAGCTTCAG AAACTGCAGC AGCTGAAAGA TGAAACTGGA 
GAATTGTCAT CTGCTGATGA GAAGCGTTAT CGTGCACTGA AGCGAACAGC AGAACGTGAA 
TTACTTATGA ATGCAGATGT TATCTGCTGC ACGTGTGTGG GAGCAGGTGA CCCGAGACTT 
GCGAAAATGC AGTTCCGCTC CATTTTAATT GATGAAAGCA CGCAGGCTAC TGAACCGGAG 
TGTATGGTGC CCGTGGTTCT TGGAGCAAAG CAGCTGATCC TCGTCGGTGA CCACTGCCAG 
CTGGGTCCTG TTGTGATGTG TAAGAAAGCT GCAAAGGCAG GCCTGTCTCA GTCTCTCTTT 
GAGAGGCTTG TGGTGTTGGG GATCCGTCCA ATCCGCCTGC AAGTGCAGTA TCGGATGCAC 
CCTGCACTTA GTGCTTTTCC ATCCAACATC TTCTACGAGG GCTCTCTCCA GAACGGCGTC 
ACTGCAGCGG ACCGTGTGAA AAAGGGATTT GATTTCCAGT GGCCCCAGCC AGATAAGCCG 
ATGTTCTTCT ATGTGACCCA GGGGCAGGAA GAAATTGCCA GTTCAGGCAC CTCTTACTTG 
AACAGGACGG AAGCTGCTAA TGTGGAGAAG ATAACTACAA AACTGTTGAA AGCTGGTGCC 
AAACCAGATC AGATTGGCAT TATTACACCC TACGAGGGCC AGCGATCTTA CCTGGTGCAG 
TACATGCAGT TCAGCGGGTC CTTGCATACA AAGCTCTACC AGGAAGTGGA AATCGCAAGT 
GTCGATGCTT TCCAGGGACG GGAGAAGGAT TTTATTATCC TTTCTTGCGT AAGAGCCAAT 
GAGCACCAAG GAATTGGATT TTTAAATGAT CCCAGACGTC TTAACGTGGC TCTTACCAGA 
GCAAGATATG GAGTCATTAT TGTTGGGAAC CCAAAGGCTC TGNNNNNNNN NNNNNNNNNN 
NNNNNNNNNN NGCTGAACTA CTACAAAGAG CAGAAGGTGC TGGTAGAGGG ACCACTGAAC 
AACCTCCGGG AAAGCCTCAT GCAGTTCAGC AAGCCTCGTA AACTCGTCAA TACCATCAAC 
CCTGGTGCCC GTTTCATGAC GACTGCCATG TATGACGCAC GTGAAGCTAT TATCCCAGGA 
TCTGTCTATG ACAGGAGCAG TCAAGGGCGC CCATCCAACA TGTACTTCCA GACTCACGAC 
CAGATCGGGA TGATTAGTGC TGGGCCCAGC CACGTCCCTG CGATGAACAT TCCTATCCCT 
TTCAATCTCG TCATGCCACC CATGCCGCCC CCAGGATACT TCGGGCAGGC CAATGGCCCG 
GCGGCAGGAC GCGGCACACC CAAAGGGAAG ACTGGCCGTG GCGGGCGCCA GAAAAACCGT 
TTTGGGATTC CTGGAACTAG CCAGTCAAAC CTTCCCAACA GTCAAGCGAG CCAAGATGTG 
GTGTCCCAGC CTTTCTCCCA GGGTCCACTG ACTCAGGGCT ATATCTCCAT GAGTCAGCCG 
TCGCAGATGA GCCAGCCAGG ACTCTCCCAG CCAGAATTAT CACAGGACAG TTACCTTGGT 
GATGAGTTTA AATCTCAGAT TGATGTGGCG CTGTCACAGG ATTCTACCTA CCAGGGTGAA 
CGTGCATATC AACACGGTGG AGTGACGGGA TTGTCACAGT ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025066110.1
CDS	1..3165
Translation	MEYSFVVQGVKVNGPDGILQNGAVDDNVAKTSQLLAELNFEEDEEDTYYTKDLPVHACSYCGIHDPACVVYCNTSKKWFCNGRGNTSGSHIVNHLVRAKCKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCRQPCASQSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQITAQQINKLEELWKENPSATLEDLEKPGVDEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAYFTLPKTDSDMRLMQGDEICLRYKGDLAPLWKGIGHVIKVPDNYGDEIAIELRSSVGAPVEVTHNFQVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVIIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQRPLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDSPVSFLALHNQIRNMDSMPELQKLQQLKDETGELSSADEKRYRALKRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRPIRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRVKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTKLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALnullnullnullnullnullnullnullnullnullnullLNYYKEQKVLVEGPLNNLRESLMQFSKPRKLVNTINPGARFMTTAMYDAREAIIPGSVYDRSSQGRPSNMYFQTHDQIGMISAGPSHVPAMNIPIPFNLVMPPMPPPGYFGQANGPAAGRGTPKGKTGRGGRQKNRFGIPGTSQSNLPNSQASQDVVSQPFSQGPLTQGYISMSQPSQMSQPGLSQPELSQDSYLGDEFKSQIDVALSQDSTYQGERAYQHGGVTGLSQY

Target ORF information:

RefSeq Version	XM_025210325.1
Organism	Alligator sinensis(Chinese alligator)
Definition	Alligator sinensis UPF1, RNA helicase and ATPase (UPF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025210325.1

ORF Insert Sequence:

ATGGAATACA GTTTTGTGGT GCAGGGTGTT AAAGTTAATG GACCTGATGG CATCCTACAG 
AACGGTGCCG TGGATGATAA TGTGGCTAAA ACCAGCCAAC TTCTGGCAGA GTTGAACTTT 
GAAGAAGATG AAGAAGACAC CTATTATACC AAGGATCTTC CTGTGCATGC TTGCAGTTAC 
TGTGGCATTC ATGACCCAGC CTGCGTGGTT TACTGCAACA CCAGCAAAAA GTGGTTCTGC 
AATGGGCGTG GCAATACATC CGGCAGCCAC ATTGTAAACC ATCTGGTGAG AGCCAAATGC 
AAGGAGGTAA CTCTCCACAA AGACGGGCCT CTTGGAGAGA CTGTCTTGGA GTGCTACAAT 
TGTGGATGCC GGAACGTGTT CCTCCTTGGC TTCATTCCTG CAAAGGCTGA CTCGGTAGTA 
GTCTTGCTGT GCAGGCAACC ATGTGCCAGT CAGAGCAGTT TAAAGGATAT TAATTGGGAC 
AGTTCACAGT GGCAGCCTCT TATCCAAGAC CGCTGCTTCC TTTCCTGGCT GGTAAAGATT 
CCCTCTGAAC AGGAGCAGCT GCGAGCCCGT CAGATCACAG CTCAGCAAAT TAACAAACTG 
GAGGAACTCT GGAAGGAAAA CCCCTCTGCA ACCCTTGAAG ACTTGGAAAA ACCTGGTGTT 
GATGAAGAAC CGCAGCATGT CTTACTTCGA TATGAGGATG CTTATCAGTA CCAAAATATC 
TTTGGACCTT TAGTTAAGCT GGAGGCAGAC TATGACAAGA AACTCAAGGA ATCCCAGACC 
CAGGATAACA TCACAGTGAG ATGGGACCTA GGCTTAAACA AGAAGAGAAT TGCTTACTTC 
ACTTTGCCAA AGACTGACTC AGATATGCGT CTTATGCAAG GAGATGAGAT CTGCCTGAGG 
TATAAAGGAG ACCTGGCCCC TCTGTGGAAA GGAATTGGTC ATGTTATCAA AGTCCCGGAT 
AATTATGGTG ATGAGATAGC CATTGAGCTG AGAAGCAGCG TTGGAGCACC TGTGGAGGTT 
ACTCATAACT TCCAGGTGGA TTTTGTTTGG AAGTCTACTT CATTTGACAG AATGCAAAGT 
GCTTTAAAAA CATTTGCAGT GGATGAAACT TCTGTGTCTG GCTACATCTA CCACAAGCTG 
TTAGGTCATG AGGTAGAAGA TGTGATTATT AAATGCCAGC TGCCAAAGCG TTTTACAGCG 
CAAGGACTGC CTGACCTCAA CCATTCTCAG GTTTATGCTG TGAAGACTGT CTTGCAGCGT 
CCTCTGAGCC TTATCCAAGG TCCCCCCGGC ACAGGAAAAA CAGTGACATC AGCAACGATT 
GTCTACCATC TAGCTAGGCA GGGCAATGGG CCCGTGTTAG TTTGTGCCCC AAGTAACATA 
GCTGTGGATC AGCTGACCGA GAAGATTCAT CAGACGGGAC TGAAAGTGGT TCGCCTGTGT 
GCTAAAAGTC GTGAGGCAAT CGATTCTCCT GTATCTTTCT TGGCATTGCA CAACCAGATC 
AGAAACATGG ACAGTATGCC TGAGCTTCAG AAACTGCAGC AGCTGAAAGA TGAAACTGGA 
GAATTGTCAT CTGCTGATGA GAAGCGTTAT CGTGCACTGA AGCGAACAGC AGAACGTGAA 
TTACTTATGA ATGCAGATGT TATCTGCTGC ACGTGTGTGG GAGCAGGTGA CCCGAGACTT 
GCGAAAATGC AGTTCCGCTC CATTTTAATT GATGAAAGCA CGCAGGCTAC TGAACCGGAG 
TGTATGGTGC CCGTGGTTCT TGGAGCAAAG CAGCTGATCC TCGTCGGTGA CCACTGCCAG 
CTGGGTCCTG TTGTGATGTG TAAGAAAGCT GCAAAGGCAG GCCTGTCTCA GTCTCTCTTT 
GAGAGGCTTG TGGTGTTGGG GATCCGTCCA ATCCGCCTGC AAGTGCAGTA TCGGATGCAC 
CCTGCACTTA GTGCTTTTCC ATCCAACATC TTCTACGAGG GCTCTCTCCA GAACGGCGTC 
ACTGCAGCGG ACCGTGTGAA AAAGGGATTT GATTTCCAGT GGCCCCAGCC AGATAAGCCG 
ATGTTCTTCT ATGTGACCCA GGGGCAGGAA GAAATTGCCA GTTCAGGCAC CTCTTACTTG 
AACAGGACGG AAGCTGCTAA TGTGGAGAAG ATAACTACAA AACTGTTGAA AGCTGGTGCC 
AAACCAGATC AGATTGGCAT TATTACACCC TACGAGGGCC AGCGATCTTA CCTGGTGCAG 
TACATGCAGT TCAGCGGGTC CTTGCATACA AAGCTCTACC AGGAAGTGGA AATCGCAAGT 
GTCGATGCTT TCCAGGGACG GGAGAAGGAT TTTATTATCC TTTCTTGCGT AAGAGCCAAT 
GAGCACCAAG GAATTGGATT TTTAAATGAT CCCAGACGTC TTAACGTGGC TCTTACCAGA 
GCAAGATATG GAGTCATTAT TGTTGGGAAC CCAAAGGCTC TGNNNNNNNN NNNNNNNNNN 
NNNNNNNNNN NGCTGAACTA CTACAAAGAG CAGAAGGTGC TGGTAGAGGG ACCACTGAAC 
AACCTCCGGG AAAGCCTCAT GCAGTTCAGC AAGCCTCGTA AACTCGTCAA TACCATCAAC 
CCTGGTGCCC GTTTCATGAC GACTGCCATG TATGACGCAC GTGAAGCTAT TATCCCAGGA 
TCTGTCTATG ACAGGAGCAG TCAAGGGCGC CCATCCAACA TGTACTTCCA GACTCACGAC 
CAGATCGGGA TGATTAGTGC TGGGCCCAGC CACGTCCCTG CGATGAACAT TCCTATCCCT 
TTCAATCTCG TCATGCCACC CATGCCGCCC CCAGGATACT TCGGGCAGGC CAATGGCCCG 
GCGGCAGGAC GCGGCACACC CAAAGGGAAG ACTGGCCGTG GCGGGCGCCA GAAAAACCGT 
TTTGGGATTC CTGGAACTAG CCAGTCAAAC CTTCCCAACA GTCAAGCGAG CCAAGATGTG 
GTGTCCCAGC CTTTCTCCCA GGGTCCACTG ACTCAGGGCT ATATCTCCAT GAGTCAGCCG 
TCGCAGATGA GCCAGCCAGG ACTCTCCCAG CCAGAATTAT CACAGGACAG TTACCTTGGT 
GATGAGTTTA AATCTCAGAT TGATGTGGCG CTGTCACAGG ATTCTACCTA CCAGGGTGAA 
CGTGCATATC AACACGGTGG AGTGACGGGA TTGTCACAGT ATTAG